Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| human papillomaviruses in amerindian women from brazilian amazonia. | we evaluated the prevalence of human papillomavirus (hpv) infection in amerindian women from a tribe in brazilian amazonia. demographic data, pap smears and cervical samples for hpv dna detection by polymerase chain reaction (pcr) were obtained for women aged above 10 years old. in total, 79 (85.9%) out of 92 eligible women who lived there were interviewed; all women already had engaged in sexual activity. seventy-eight and 49 women allowed collection of pap smears and pcr samples, respectively. ... | 2002 | 12113494 |
| igfbp-3 mediates p53-induced apoptosis during serum starvation. | insulin-like growth factor binding protein (igfbp)-3, a p53-response gene, can induce apoptosis in an igf-independent manner. here we demonstrate that igfbp-3 mediates p53-induced apoptosis during serum starvation using two foil neoplastic cell models: one which introduces p53 activity and one which eliminates it. we created a doxycycline-inducible p53 model from the p53-negative pc-3 prostate cancer cell line. doxycycline treatment increased both p53 and igfbp-3 levels. it also augmented apopto ... | 2002 | 12118329 |
| human papillomavirus: a review. | human papillomavirus infection remains a great source of morbidity and mortality. progress in understanding the structure of hpv and its pathogenesis has led to a wide variety of possible new treatment modalities to combat hpv-related disease. most hpv infections (whether high risk or low risk) resolve without any medical intervention. persistent or progressive disease, however, remains difficult to treat. although currently available therapies have proved efficacious and tolerable in the treatm ... | 2002 | 12120445 |
| adenovirus e1-transformed cells grow despite the continuous presence of transcriptionally active p53. | the e1 region of adenovirus (ad) type 5 is capable of transforming cells. according to current concepts, the ad e1b 55 kda (e1b 55k) protein enables transformed cells to grow by constantly binding and inactivating the p53 tumour suppressor protein. to test this model, the transcriptional activity of p53 was determined in ad e1-transformed cells. surprisingly, it was found that a p53-responsive promoter is highly active in ad e1-transformed cells and further activated only 3- to 4-fold (compared ... | 2002 | 12124469 |
| oncogenic human papillomavirus e6 proteins target the magi-2 and magi-3 proteins for degradation. | the e6 proteins from the high-risk human papillomavirus (hpv) types have previously been shown to target a number of pdz domain-containing proteins for proteasome-mediated degradation. these include the hdlg tumour suppressor and the magi-1 protein. in this study we show that high-risk hpv e6 proteins also target the related magi-2 and magi-3 proteins for degradation. moreover, we show that the interaction is specific to one pdz domain, and that co-expression of this domain can protect each of t ... | 2002 | 12140759 |
| clinical and economic implications of adding hpv tests to the routine cytology follow-up and management of patients with histologically defined cervical intraepithelial neoplasia grade 1. | the objective of this study was to evaluate the clinical and economic implications of adding human papillomavirus (hpv) testing to the follow-up and management protocol of women with a histological diagnosis of low-grade cervical intraepithelial neoplasia (cin1). | 2002 | 12144817 |
| therapeutic vaccination against chronic viral infections. | chronic viral infections such as those caused by hepatitis b virus, human papilloma virus, herpes simplex virus, and hiv, in theory, present logical targets of active specific immunotherapy. indeed, immunological mechanisms are involved in several aspects of their pathogenesis and natural course, such as virus persistence, destruction of infected cells and control of viral replication. therapeutic vaccination could therefore be an adequate replacement for, or adjunct to, existing therapies. almo ... | 2002 | 12144898 |
| imiquimod 5% cream in the treatment of human papillomavirus-16-positive erythroplasia of queyrat. | erythroplasia of queyrat is a human-papillomavirus-associated carcinoma in situ of the penis. imiquimod, a new topically applied immunomodulatory agent, has been successfully used in the treatment of anogenital warts. we report the complete clearance of hpv-16-positive erythroplasia of queyrat with 5% imiquimod cream. | 2002 | 12145439 |
| colourimetric signal amplification of in situ hybridization assay for human papillomavirus dna detection in cytological samples. | signal amplification of in situ hybridization (ish) can be obtained by catalyzed reporter deposition (card), the catalyzed deposition of biotinylated tyramide at the location of the labelled probe. we analyzed 156 cervico/vaginal samples in order to evaluate whether ish-card improves conventional ish technique in detecting human papillomavirus (hpv) on cytological smears and can be used in clinical practice. 126 patients were clinically suspected to have a squamous intraepithelial lesions (sil) ... | 2002 | 12148585 |
| [human papilloma virus in quechua women from jujuy with high frequency of cervical cancer: viral types and hpv-16 variants]. | human papillomaviruses (hpvs) are etiologically associated to cervical carcinoma. in order to evaluate hpv infection and its relationship with the high frequency of this neoplasia in quechua women from jujuy (argentina), 271 cervical samples from preneoplastic and neoplastic lesions (biopsies) and normal controls (cytologies) were studied. detection and typing were performed using pcr-rflp or pcr-hybridization and the hpv-16 variability in l1 and e6 genes (by pcr-hybridization) was analysed. hpv ... | 2002 | 12150002 |
| human papillomavirus: emerging trends in detection and management. | cervical cancer continues to be a major epidemiologic problem in developed and underdeveloped nations alike. current screening practices rely on cervical cytology for the early detection of cervical neoplasms. it has now been demonstrated that over 99% of cervical carcinomas are positive for human papillomavirus (hpv) dna. this article reviews the currently available methods of hpv detection as well as emerging strategies for the implementation of hpv testing into clinical practice. | 2002 | 12150752 |
| cervicovaginal coinfections with human papillomavirus and chlamydia trachomatis. | human papillomavirus (hpv) and chlamydia trachomatis (ct) are the two most common sexually transmitted pathogens, and infection with either reportedly was associated with cervical intraepithelial neoplasia (cin) in women. in view of their similar mode of transmission, ct infection was examined as a possible hpv cofactor in the etiology of cin disease. in total, 129 women were included in the study, of whom 80 were negative (mean age 34.17 +/- 6.9) and 49 were positive (mean age 33.16 +/- 6.8) fo ... | 2002 | 12151187 |
| the other epidemics. sexually transmitted diseases. | around 70% of female infertility in developing countries is caused by sexually transmitted diseases (stds) that can be traced back to husbands or partners. stds and reproductive tract infections cause 750,000 deaths and 75 million illnesses among women each year worldwide, and these deaths may more than double by the year 2000. death rates are rising fastest in africa, followed by asia and latin america. about 450,000 cases of potentially fatal reproductive tract cancers are diagnosed annuall ... | 1993 | 12159277 |
| insulin-like growth factor-binding protein 3 expression increases during immortalization of cervical keratinocytes by human papillomavirus type 16 e6 and e7 proteins. | human papillomaviruses (hpvs) infect cervical epithelial cells and induce both benign and precancerous lesions. high-risk hpvs promote the development of cervical cancer in vivo and can immortalize cervical epithelial cells in vitro, whereas low-risk hpvs cannot. we used cdna microarrays and quantitative polymerase chain reaction to compare cellular gene expression in primary cervical epithelial cells during a time course after retroviral transduction with either low-risk or high-risk e6/e7 gene ... | 2002 | 12163384 |
| down regulation of the interleukin-8 promoter by human papillomavirus type 16 e6 and e7 through effects on creb binding protein/p300 and p/caf. | previously, we reported that human papillomavirus (hpv) type 16 e6 binds to c/h1, c/h3, and the c-terminal domains of coactivators p300 and cbp, causing the modulation of the transcription of certain genes controlled by nf-kappab (p65 or rela) and p53. to establish the biological significance of these observations, we have focused on the transcriptional regulation of interleukin-8 (il-8), a potent chemoattractant for t lymphocytes and neutrophils, which is also essential for the initiation of th ... | 2002 | 12163591 |
| viral interferon regulatory factor 1 of kaposi's sarcoma-associated herpesvirus interacts with a cell death regulator, grim19, and inhibits interferon/retinoic acid-induced cell death. | kaposi's sarcoma-associated herpesvirus (kshv) plays a significant role in the development of kaposi's sarcoma, primary effusion lymphoma, and some forms of multicentric castleman's disease. the kshv open reading frame k9 encodes the viral interferon (ifn) factor 1 (virf1), which downregulates ifn- and irf-mediated transcriptional activation, and leads to cellular transformation in rodent fibroblasts and induction of tumors in nude mice. using the yeast two-hybrid assay, we identified genes asso ... | 2002 | 12163600 |
| intranasal vaccination with a recombinant vesicular stomatitis virus expressing cottontail rabbit papillomavirus l1 protein provides complete protection against papillomavirus-induced disease. | immunizations with live recombinant vesicular stomatitis viruses (rvsv) expressing foreign viral proteins have successfully protected animals from challenges with several heterologous viruses. we developed an rvsv expressing the major capsid protein (l1) of cottontail rabbit papillomavirus (crpv) and tested the efficacy of protection following crpv challenge. an rvsv expressing l1 of crpv (vsv-l1) was characterized for the protective ability afforded by intranasal, intradermal, or intramuscular ... | 2002 | 12163609 |
| nutritional and genetic inefficiencies in one-carbon metabolism and cervical cancer risk. | folate deficiency has long been postulated to play a role in the etiology of cervical cancer, the third most frequent cancer among women worldwide. in a large, multiethnic community-based case-control study of invasive cervical cancer in five u.s. areas, we assessed accepted and postulated risk factors with an in-home interview and successfully obtained blood samples, at least 6 mo after completion of cancer treatment, from 51 and 68%, respectively, of interviewed cases and controls. cases with ... | 2002 | 12163690 |
| expression of p21/waf-1, status of apoptosis and p53 mutation in esophageal squamous cell carcinoma with hpv infection. | human papilloma virus (hpv) is regarded as a causative carcinogenic agent in anogenital squamous cell carcinoma (scc), but there is controversy about its etiologic role in esophageal scc (escc). in this study, we attempted to clarify whether hpv infection plays a crucial role in the development of escc by analysis of multiple factors. these included: detection of hpv dna; evaluation of immunohistochemical assays for hpv-related cell cycle regulators and apoptosis by the terminal deoxynucleotidyl ... | 2002 | 12167102 |
| human papillomavirus type 38 infection in oral squamous cell carcinomas. | in this study, 53 paraffin-embedded oral squamous cell carcinoma (oscc) biopsy specimens were used. human papillomavirus type 38 (hpv-38) infection was demonstrated in osccs using the pcr technique, dna sequencing analysis, in situ hybridization, and immunohistochemical techniques. additionally, the correlation between hpv-38 infection and expressions of proliferating cell nuclear antigens (pcna) or p53 protein was analyzed immunohistochemically. using consensus primers for the l1 region (l1-pcr ... | 2002 | 12167437 |
| recurrent respiratory papillomatosis. | recurrent respiratory papillomatosis is a relatively rare disease caused by members of the human papilloma virus family. lesions may occur anywhere throughout the respiratory tract but show a predilection for squamo-columnar epithelial junctions, frequently leading to hoarseness and upper airway obstruction. rarely, it can progress to squamous cell carcinoma. the impact of recurrent respiratory papillomatosis on patients, their families, and the health care system is considerable. unfortunately, ... | 2002 | 12169123 |
| identification of a novel activity of human papillomavirus type 16 e6 protein in deregulating the g1/s transition. | in this study we show that e6 of human papillomavirus has the ability to deregulate the cell cycle g1/s transition. in rodent immortalized fibroblasts (nih3t3) serum deprivation or over-expression of the cyclin-dependent kinase inhibitors, p16(ink4a) or p27(kip1), leads to g1 cell cycle arrest. hpv16 e6 overcomes the antiproliferative signals, gaining the ability to drive serum-deprived and p16(ink4a) or p27(kip1) over-expressing cells into s phase. e6 protein from the benign hpv type 1 displays ... | 2002 | 12173036 |
| common skin disorders of the penis. | diseases of the male genitalia range from infectious lesions to inflammatory and neoplastic conditions, including many genital manifestations of more general skin diseases. this review highlights the clinical features, diagnosis and treatment of the most common dermatoses of the male genitalia. herpes genitalis and infections caused by human papillomavirus (hpv) are increasing, particularly in young sexually active people. herpes simplex virus infection is the commonest infectious cause of genit ... | 2002 | 12175386 |
| [human papilloma virus (hpv) infection in the pathology of the endometrium. role of immunological activation in antiviral and antineoplastic defense]. | endometrial cancer is one of the most prevalent neoplastic diseases in women, in the developed countries. in spite of hormonal dysfunction, no pivotal mechanism leading to this pathology is known. the study design was to evaluate the presence of human papilloma virus within the endometrium by in situ hybridization and its potential role in the occurrence of dysplastic and neoplastic endometrial lesions. immunological antiviral and antitumour defense was investigated by cytometric measurement of ... | 2002 | 12177512 |
| fluctuations in prevalence of cervical human papillomavirus in women frequently sampled during a single menstrual cycle. | in the last few years much attention has been focused on the implementation of human papillomavirus detection in population based screening programmes to identify women at risk for cervical cancer. short-term fluctuations in prevalence of human papillomavirus were investigated within a single menstrual cycle. the highest prevalence was found at the follicular phase (55%), whereas the cumulative prevalence was 75%. | 2002 | 12177771 |
| reduced apoptotic levels in squamous but not basal cell carcinomas correlates with detection of cutaneous human papillomavirus. | we have investigated the apoptotic levels and expression of the apoptotic inducer bak in non-melanoma skin cancers. squamous cell carcinomas of known human papillomavirus status from immunocompetent patients were analysed for the expression of the bak protein, and the expression profile was compared both to the presence of apoptotic cells and the proliferation marker ki-67. we demonstrate an inverse correlation between human papillomavirus positivity and bak expression in squamous cell carcinoma ... | 2002 | 12177802 |
| updates and controversies in the diagnosis and treatment of cin. | this paper presents the discourse of dr. genera a. manuel-limson entitled "updates and controversies in the diagnosis and treatment of cervical intraepithelial neoplasia (cin)." manuel-limson's discussion on diagnostic procedures for cin includes pap smear, colposcopy, cervicography, human papilloma virus testing and endocervical curettage (ecc). it is noted that pap smear is the most cost-effective cancer screening at present. however, there are cases such as the screening for high-grade squ ... | 1999 | 12179671 |
| sexually transmitted diseases treatment guidelines 2002. centers for disease control and prevention. | these guidelines for the treatment of patients who have sexually transmitted diseases (stds) were developed by the centers for disease control and prevention (cdc) after consultation with a group of professionals knowledgeable in the field of stds who met in atlanta on september 26-28, 2000. the information in this report updates the 1998 guidelines for treatment of sexually transmitted diseases (mmwr 1998;47 [no. rr-1]). included in these updated guidelines are new alternative regimens for scab ... | 2002 | 12184549 |
| p53 immunohistochemical expression: messages in cervical carcinogenesis. | the pattern of p53 expression was studied in pre-invasive and invasive cervical carcinoma in an attempt to clarify its role in cervical carcinogenesis. | 2002 | 12190289 |
| present standards for cervical cancer screening. | survival rates for cancer of the uterine cervix have improved over the last 40 years largely because of the impact of screening measures such as the pap smear. the ability to screen and treat women for preinvasive disease, cervical dysplasia, is the key factor leading to the reduction in the incidence of invasive cervical cancer. more recently, the ability to test women for the causative agent, the human papilloma virus, has emerged as a potential screening tool. new research has focused on new ... | 2002 | 12192269 |
| frequency of herpes simplex virus, cytomegalovirus and human papillomavirus dna in semen. | herpes simplex virus (hsv-2) and cytomegalovirus (cmv) infections produce brain damage in the newborn, and human papillomavirus (hpv) plays a role in cervical carcinogenesis. to assess the frequency of herpes virus and hpv in semen and its role in transmission, semen from 111 male partners of women with histologically-detected genital hpv infection was analysed for hsv, cmv and hpv infection. we used cell culture to detect hsv and cmv, and polymerase chain reaction (pcr) for hpv. virological fin ... | 2002 | 12194737 |
| comprehensive analysis of human leukocyte antigen class i alleles and cervical neoplasia in 3 epidemiologic studies. | to comprehensively explore the relationship between human leukocyte antigen (hla) class i alleles and cervical neoplasia, a subset of participants from 3 large us and costa rican cervix studies were typed for hla class i alleles. study subjects were women with cervical cancer or high-grade squamous epithelial lesions (hsils; n=365) or low-grade squamous epithelial lesions (lsils; n=275) or who were cytologically normal (control subjects; n=681). allele-disease associations were assessed by logis ... | 2002 | 12195346 |
| incidence, prevalence, and clearance of type-specific human papillomavirus infections: the young women's health study. | the natural history of type-specific human papillomavirus (hpv) infections was examined in a cohort of 331 women aged 18-35 years who self-referred for routine gynecological care. participants underwent a gynecological examination at baseline and at approximately 4 and approximately 10 months after baseline. cervical samples were collected for hpv testing and genotyping at each visit, as was information on reproductive, sexual, and medical histories. the rate of new hpv infections was 2.9% per m ... | 2002 | 12195372 |
| human papillomavirus molecular biology and pathogenesis. | human papillomavirus (hpv) infection is the most common sexually transmitted disease in the world and accounts for an estimated 11% of the global cancer incidence in women. hpv-16 is the most prevalent type detected in cervical cancer and along with types 18, 31, 33 and 45 has been classified as a class i carcinogen. in addition to cervical cancer, hpvs are also associated with the malignant transformation of other mucosal and skin cancers. thus, the combination of the malignant potential of hpv ... | 2002 | 12195562 |
| serum selenium and the risk of cervical cancer among women in the united states. | to explore the relationship between serum selenium and cervical cancer. | 2002 | 12195641 |
| penile lesions and human papillomavirus in male sexual partners of women with cervical intraepithelial neoplasia. | genital human papillomavirus infection (hpv) is causally associated with cervical carcinomas and premalignant lesions. limited information is available about the prevalence of hpv and penile lesions in male sexual partners of women with cervical intraepithelial neoplasia (cin). | 2002 | 12196743 |
| variable expression of some "housekeeping" genes during human keratinocyte differentiation. | we investigated the expression levels of four cellular "housekeeping" genes during epithelial differentiation. differentiation is a dynamic process and various cellular rnas have been targeted for use as internal controls during differentiation of human keratinocytes, but the consistent expression of such standards has not been previously validated. we used the organotypic (raft) culture system to grow stratified and differentiated epithelium in vitro. we compared cellular rnas from epithelial t ... | 2002 | 12202253 |
| selective silencing of viral gene expression in hpv-positive human cervical carcinoma cells treated with sirna, a primer of rna interference. | selective silencing of mammalian gene expression has recently been achieved using short interfering rna (sirna). synthetic sirna targets homologous mrna for degradation and the process is highly efficient. here we demonstrate sirna silencing of pathogenic viral gene expression. as a well characterized model we chose cervical carcinoma cells positive for human papillomavirus type 16. over 90% of human cervical cancers are positive for papillomavirus and abnormal cell proliferation is driven by co ... | 2002 | 12203116 |
| long-term follow-up of women with atypical squamous cells of undetermined significance (ascus). | ascus is the most common epithelial abnormality diagnosed in cytology laboratories in the us. recently, the clinical importance of this diagnosis has been seriously questioned, with some investigators advocating elimination of this diagnostic category. this might be inappropriate if the ascus designation does define a population that is at significant risk for the development of dysplasia. cytology and surgical pathology reports for all patients diagnosed as ascus in our laboratory during 1990 w ... | 2002 | 12203862 |
| reply to commentary by drs majewski & jablonska, "human papillomavirus and oncogenesis: critical evaluation of recent findings". | 2002 | 12207775 | |
| the colon anion transporter, down-regulated in adenoma, induces growth suppression that is abrogated by e1a. | the down-regulated in adenoma (dra) gene is significantly down-regulated in adenomas and adenocarcinomas of the colon as well as in colon cancer cell lines. it is also mutated in the disease congenital chloride diarrhea, which is characterized by loss of chloride transport and diarrhea. we now show a second function for dra relevant to colon tumorigenesis, i.e., growth suppression. transfection of full-length dra into various cell lines (dld-1, ht-29, hct-15, sw837, sw480, mcf-7, nih3t3, caski, ... | 2002 | 12208765 |
| the upstream regulatory region of human papillomavirus type 31 is insensitive to glucocorticoid induction. | the upstream regulatory region (urr) of various types of human papillomaviruses (hpvs) has been shown to contain functional glucocorticoid response elements (gres), including hpv type 11 (hpv11), hpv16, and hpv18. glucocorticoids have been demonstrated to induce the transcriptional activity of the early promoters of these hpv types. although it has been assumed that the urr of hpv31 contains at least one gre, no functionality has been demonstrated. we attempt to show here inducibility of the urr ... | 2002 | 12208949 |
| human telomerase and its regulation. | the telomere is a special functional complex at the end of linear eukaryotic chromosomes, consisting of tandem repeat dna sequences and associated proteins. it is essential for maintaining the integrity and stability of linear eukaryotic genomes. telomere length regulation and maintenance contribute to normal human cellular aging and human diseases. the synthesis of telomeres is mainly achieved by the cellular reverse transcriptase telomerase, an rna-dependent dna polymerase that adds telomeric ... | 2002 | 12208997 |
| human papillomavirus genotype prevalence in high-grade squamous intraepithelial lesions and colposcopically normal women from zimbabwe. | 2002 | 12209615 | |
| escape of p53 protein from e6-mediated degradation in hela cells after cisplatin therapy. | we previously reported that therapy of human cervical carcinoma hela cells with cp induced segregation of nucleoli and changes of nuclei characteristic of apoptosis. we raised the question of whether p53 can be reactivated by chemotherapy in hela cells despite the presence of hpv-encoded e6 activity. cellular levels of p53 protein increased after cp treatment, reaching a maximum after 6 hr. p53 protein accumulated preferentially in the nucleoli, with a peak after 15 hr. cp-induced nucleolar targ ... | 2002 | 12209989 |
| content of folic acid and free homocysteine in blood serum of human papillomavirus-infected women with cervical dysplasia. | the authors estimated the concentrations of folic acid and free homocysteine in the blood serum of women with cin iii (cervical intraepithelial neoplasia-burghard's classification) infected with dna hpv (human papillomaviruses) of type 16 and/or 18. the control group consisted of 49 patients with normal cytological smears without hpv infection. types 16 and/or 18 dna hpv were found in 50 patients. this women qualified for the studied group. the sequence of dna hpv type 16 and/or 18 was identifie ... | 2002 | 12214730 |
| validation of flt uptake as a measure of thymidine kinase-1 activity in a549 carcinoma cells. | the thymidine analog (18)f-3'-deoxy-3' -fluorothymidine (flt) is being used clinically for pet imaging of tumor proliferation. appropriate use of this tracer requires validating the mechanisms by which it accumulates in dividing cells. we tested the accuracy with which flt uptake predicted the activity of cytosolic thymidine kinase-1 (tk(1)), an enzyme that is upregulated before and during dna synthesis. | 2002 | 12215561 |
| genetic alterations in cervical carcinomas: frequent low-level amplifications of oncogenes are associated with human papillomavirus infection. | the development of cervical carcinoma is closely associated with hpv infection. however, other genetic alterations also play an important role. in this study, we analyzed copy number alterations of several oncogene loci in a panel of 84 cervical tumors. sixty-five (77%) tumors were hpv dna-positive, and most were infected with type 16 or type 18 or both. the oncogenes studied include pik3ca at 3q26.3, tert at 5p15.33, c-myc at 8q24, ccnd1 at 11q13.3, erbb2 at 17q21.2 and locus region 20q13.2. am ... | 2002 | 12216070 |
| papillomaviruses: death-defying acts in skin cancer. | ultraviolet (uv) radiation is an environmental agent that has a major impact on humans, and cumulative exposure poses a serious risk in terms of developing skin cancer. acute doses of uv induce apoptotic cell death in the skin via signalling pathways that are, in part, dependent on the p53 tumour suppressor protein. however, p53-independent mechanisms have also been described. recent findings show that a high proportion of non-melanoma skin cancers contain human papillomavirus. the viral e6 prot ... | 2002 | 12223312 |
| vitamin a, carotenoids, and risk of persistent oncogenic human papillomavirus infection. | oncogenic human papillomavirus (hpv) infection is the main etiologic factor for cervical neoplasia, although infection alone is insufficient to produce disease. cofactors such as nutritional factors may be necessary for viral progression to neoplasia. results from previous studies have suggested that higher dietary consumption and circulating levels of certain micronutrients may be protective against cervical neoplasia. this study evaluated the role of vitamin a and carotenoids on hpv persistenc ... | 2002 | 12223432 |
| differences in factors associated with oncogenic and nononcogenic human papillomavirus infection at the united states-mexico border. | previous studies have shown that risk factors for oncogenic (high-risk) and nononcogenic (low-risk) human papillomavirus (hpv) infection differ. to determine the risk factors for oncogenic and nononcogenic hpv infection in women residing along the united states-mexico border, a cross-sectional study of type-specific hpv prevalence was conducted. women (n=2246) ages 15-79 years, living in communities on both sides of the united states-mexico border and attending family planning clinics were recru ... | 2002 | 12223441 |
| detection of human papillomavirus in esophageal carcinoma. | the aim of the study was to assess the prevalence of human papillomavirus (hpv) in the esophagus in the coastal region of eastern guangdong, southern china, an area with a high incidence of esophageal carcinoma. fresh surgical resection esophageal specimens were obtained from 176 esophageal carcinoma patients admitted to the tumor hospital of shantou university medical college. the samples were subjected to polymerase chain reaction (pcr) to detect hpv infection using consensus and type-specific ... | 2002 | 12226830 |
| comparisons of hpv dna detection by my09/11 pcr methods. | two modifications to the original l1 consensus primer human papillomavirus (hpv) pcr method, my09-my011, using amplitaq dna polymerase (my-taq), were evaluated for hpv dna detection on clinical specimens from a cohort study of cervical cancer in costa rica. first, hpv dna testing of 2978 clinical specimens by my09-my011 primer set, using amplitaq gold dna polymerase (my-gold) were compared with my-taq testing. there was 86.8% total agreement (kappa = 0.72, 95%ci = 0.70-75) and 69.6% agreement am ... | 2002 | 12226831 |
| type specific persistence of high risk human papillomavirus (hpv) as indicator of high grade cervical squamous intraepithelial lesions in young women: population based prospective follow up study. | to investigate the role of human papillomavirus (hpv) in the development of cervical neoplasia in women with no previous cervical cytological abnormalities; whether the presence of virus dna predicts development of squamous intraepithelial lesion; and whether the risk of incident squamous intraepithelial lesions differs with repeated detection of the same hpv type versus repeated detection of different types. | 2002 | 12228133 |
| testing for human papillomavirus in women with abnormal pap smear results. | 2002 | 12234220 | |
| prevalence and risk factors of sexually transmitted infections and cervical neoplasia in women's health clinics in nicaragua. | to determine prevalence and risk factors of sexually transmitted infections (stis), hiv, and cervical neoplasia in women attending women's health clinics in nicaragua, and to assess the potential impact of screening for these diseases. | 2002 | 12238655 |
| risk factors for genital hpv dna in men resemble those found in women: a study of male attendees at a danish std clinic. | genital infection with certain types of human papillomavirus (hpv) is the most important risk factor for cervical cancer. the male sexual partner is supposed to be the vector of the infection. however, the knowledge of risk factors for genital hpv dna in men is limited. the objective of this paper is to study the risk factors for hpv infection in men and to compare them with those found in women, including the study of whether there are different risk profiles for oncogenic and non-oncogenic hpv ... | 2002 | 12238658 |
| transcriptional activity among high and low risk human papillomavirus e2 proteins correlates with e2 dna binding. | the full-length e2 protein, encoded by human papillomaviruses (hpvs), is a sequence-specific transcription factor found in all hpvs, including cancer-causing high risk hpv types 16 and 18 and wart-inducing low risk hpv types 6 and 11. to investigate whether e2 proteins encoded by high risk hpvs may function differentially from e2 proteins encoded by low risk hpvs and animal papillomaviruses, we conducted comparative dna-binding and transcription studies using electrophoretic mobility shift assay ... | 2002 | 12239214 |
| e7 abolishes raf-induced arrest via mislocalization of p21(cip1). | the cellular response to oncogenic ras depends upon the presence or absence of cooperating mutations. in the absence of immortalizing oncogenes or genetic lesions, activation of the ras/raf pathway results in a p21(cip1)-dependent cellular arrest. the human papillomavirus oncoprotein e7 transforms primary cells in cooperation with ras and abolishes p21(cip1)-mediated growth arrest in the presence of various antimitogenic signals. here we have utilized a conditional raf molecule to investigate th ... | 2002 | 12242284 |
| [condyloma and contraception: the influence of contraception on the evolution of cervical lesions caused by papillomavirus]. | the course of human papilloma virus infections is unpredictable. some cervical lesions regress, some persist, and some evolve toward cancer in situ. the factors governing their course remain unknown. 61 women with cervical lesions caused by human papilloma virus infections were monitored to assess the possible role of oral contraceptive use in the progression or regression of their lesions. 30 of the women used combined ocs, and 31 used another contraceptive method or no method. the women w ... | 1988 | 12283008 |
| 1998 management recommendations for sexually transmitted diseases. | this article, intended for use by practitioners in india, summarizes the us centers for disease control's recommendations for the management of sexually transmitted diseases (stds). the stds presented are chancroid, genital herpes, granuloma inguinale, lymphogranuloma venereum, syphilis, urethritis, chlamydia, gonococcal infection, bacterial vaginosis, trichomoniasis, vulvovaginal candidiasis, pelvic inflammatory disease, epididymitis, human papillomavirus infection, warts, hepatitis b, and ect ... | 1999 | 12295052 |
| immunohistochemical localization of cdc6 in squamous and glandular neoplasia of the uterine cervix. | cdc6 has been extensively studied as a marker for cellular proliferation that is expressed during the normal cell cycle. recent studies indicate that cdc6 may be a marker for cervical intraepithelial neoplasia (cin) and carcinoma; however, the histologic distribution of cdc6 has not been explicitly defined. expression of cdc6 in the endocervical mucosa also remains unexplored. | 2002 | 12296751 |
| number of levels needed for diagnosis of cervical biopsies. | three levels of histologic sections are routinely prepared for small biopsies in many surgical pathology laboratories. the first level is superficial and may not be representative of the entire biopsy, and may therefore represent wasted resources and time for technologists and pathologists alike. | 2002 | 12296760 |
| microtiter format for simultaneous multianalyte detection and development of a pcr-chemiluminescent enzyme immunoassay for typing human papillomavirus dnas. | to allow multianalyte binding assays, we have developed a novel polystyrene microtiter plate containing 24 main wells, each divided into 7 subwells. we explored its clinical potential by developing a pcr-chemiluminescent immunoassay (pcr-cleia) for simultaneous detection and typing of seven high oncogenic risk human papillomavirus (hpv) dnas in one well. | 2002 | 12324480 |
| sexually transmitted diseases in africa: time for action. | sexually transmitted diseases (stds) are major public health problems which often lead to serious complications and sequelae, including infertility. infection with stds also facilitates the transmission of hiv, making the early diagnosis and care of stds integrated into other services one of the most cost-effective strategies to prevent the spread of hiv. the direct and indirect costs of stds worldwide are considerable. sub-saharan africa ranks first in std yearly incidence compared to other ... | 1998 | 12348788 |
| hpv infections and oesophageal cancer. | the first reports suggesting an involvement of human papillomavirus (hpv) in the development of both benign and malignant squamous cell tumours of the oesophagus date back to 1982. since then, a substantial amount of literature has accumulated on this subject, summarised in this review. to date, 239 oesophageal squamous cell papillomas have been analysed in 29 separate studies using different hpv detection methods, with hpv being detected in 51 (21.3%) cases. many more squamous cell carcinomas h ... | 2002 | 12354793 |
| hpv episomal copy number closely correlates with cell size in keratinocyte monolayer cultures. | w12e keratinocytes maintaining episomal copies of hpv dna were separated according to size by centrifugal elutriation. hpv dna copy number was greatly increased in the largest, most differentiated cells of the population. the large cells with the highest hpv copy number also showed evidence of endoreduplication of host cell dna. other cell lines maintaining episomal copies of hpv18 and hpv31 were also tested with all lines showing similar results. the results demonstrate that increase in hpv dna ... | 2002 | 12359435 |
| human papillomavirus type 32 does not display in vitro transforming properties. | human papillomavirus type 32 (hpv32) is one of the etiological agents of a benign oral condition, focal epithelial hyperplasia. however, the previously characterized properties of its e7 oncoprotein suggest a possible malignant nature for this virus. in this study we characterized the properties of hpv32 e6 and e7. our data show that hpv32 e7, despite its high affinity for prb, does not promote degradation of the cellular protein. in addition, hpv32 e6 does not prevent p53-mediated apoptosis and ... | 2002 | 12359456 |
| temperature variable and the efficiency of sperm mediated transfection of hpv16 dna into cells. | to pretreat sperm at various temperatures before exposure to human papillomavirus (hpv) 16 dna fragments and to assess the efficiency of hpv carrier sperm to transfect cumulus cells. | 2002 | 12364970 |
| the relevance of oncogenes as prognostic markers in cervical cancer. | to study the prevalence of the oncogenes c-myc, ifn-alpha; c-erbb2; h-ras codon 12, 13, and 61; c-fos; and e6/e7 oncogenes of human papillomavirus (hpv) 16 in patients with invasive carcinoma of the cervix and their prognostic significance, genomic dna and rna were isolated from tissues of 275 patients in singapore with nonmetastatic cervical cancer and 32 patients with normal cervix. the levels of expression of the various oncogenes were quantified by pcr using the respective primers. when the ... | 2002 | 12366664 |
| simian virus 40 small tumor antigen activates akt and telomerase and induces anchorage-independent growth of human epithelial cells. | human keratinocytes immortalized by full-length or early-region simian virus 40 (sv40) dna grow in agarose and form tumors in nude mice, in contrast to keratinocytes immortalized by the e6/e7 genes of human papillomaviruses. to determine the molecular basis for this biological difference in growth, we have used the individual sv40 oncogenes (large t antigen [lt] and small t antigen [st]) and human papillomavirus oncogenes (e6/e7) to study the progression of human epithelial cells from the nonimm ... | 2002 | 12368310 |
| cooperative activation of human papillomavirus type 8 gene expression by the e2 protein and the cellular coactivator p300. | the e2 proteins of papillomaviruses (pv) bind to the coactivator cbp/p300 as do many other transcription factors, but the precise role of cbp/p300 in e2-specific functions is not yet understood. we show that the e2 protein of human pv type 8 (hpv8) directly binds to p300. activation of hpv8 gene expression by low amounts of hpv8 e2 was stimulated up to sevenfold by coexpression of p300. the interaction between e2 and p300 may play a role in differentiation-dependent activation of pv gene express ... | 2002 | 12368347 |
| [evaluation of frequency human papillomavirus infections during pregnancy]. | the frequency of human papillomavirus (hpv) infection was estimated among pregnant women. | 2002 | 12369291 |
| [the clinical value of laboratory tests for detection of hpv infection in prevention and treatment of cervical cancer]. | the retrospective and prospective epidemiological studies were conducted in centres all over the world and showed that the main etiological factor in cervical cancer is human papillomavirus infection. the problem of detection of hpv infection is being currently under discussion. the selection of method of hpv detection in clinical samples and standardisation of laboratory tests allows for determination of diagnostic method in case of hpv infection. there are not clear guidelines for the pattern ... | 2002 | 12369300 |
| identification of two homologous antigenic peptides derived from l1 hpv-16 and 18 proteins specific for the hla-b*3901 allele. | in this work we present evidence that the homologous peptides ihsmnstil and ihsmnssil derived from l1 hpv-16 and 18 proteins respectively, and with high specificity for the allele hla-b*3901, according with an algorithm prediction program, induced t cell stimulation in patients with advanced cervical cancer positive for hpv-16 or 18 infection and for the hla-b*3901 allele. interestingly, t lymphocytes derived from a patient with hpv-18 infection and stimulated with the peptide ihsmnstil were cap ... | 2002 | 12376754 |
| establishment of an hla-a*0201 human papillomavirus type 16 tumor model to determine the efficacy of vaccination strategies in hla-a*0201 transgenic mice. | with the increasing generation of new cancer vaccine strategies, there is also an increasing demand for preclinical models that can carefully predict the efficacy of these vaccines in humans. however, the only tumor models available to study vaccines against human papillomavirus (hpv) 16 have been developed in c57bl/6 mice. to test the hla-restricted capabilities of vaccination strategies, it is important to establish a tumor model in hla-a*0201 transgenic mice. by transfecting heart lung fibrob ... | 2002 | 12384540 |
| the role of genotype-specific human papillomavirus detection in diagnosing residual cervical intraepithelial neoplasia. | we assessed prospectively whether residual cervical intraepithelial neoplasia (cin) after treatment for high-grade cin can be predicted by genotype-specific high-risk hpv (hr-hpv) detection in follow-up cervical scrapes. a broad spectrum, highly sensitive spf(10)-lipa-pcr hpv detection technique was used on cervical scrapes before large loop excision of the transformation zone (lletz), on the lletz biopsy and on follow-up scrapes of 90 patients treated for high-grade cin. hr-hpv was detected in ... | 2002 | 12385010 |
| the innate and early immune response to pathogen challenge in the female genital tract and the pivotal role of epithelial cells. | the female reproductive tract is immunologically unique in its requirement for tolerance to allogeneic sperm and, in the upper tract, to the conceptus. however, it must also be appropriately protected from, and respond to, a diverse array of sexually transmitted pathogens. some of these infections can be lethal (e.g. human immunodeficiency virus (hiv), human papilloma virus (hpv)), and others (e.g. chlamydia trachomatis and neisseria gonorrhoeae) can have potentially devastating reproductive seq ... | 2002 | 12385834 |
| human papillomavirus type 31b infection of human keratinocytes and the onset of early transcription. | human papillomaviruses (hpvs) cause a number of human tumors and malignancies, including cervical cancers. epithelial differentiation is required for the complete hpv life cycle and can be achieved using the organotypic (raft) culture system. the cin-612 9e cell line maintains episomal copies of hpv type 31b (hpv31b), an hpv type associated with cervical cancers. when grown in the raft system, cin-612 9e cells form a differentiated epithelium such that infectious virions can be synthesized. many ... | 2002 | 12388689 |
| genetic analysis of high-risk e6 in episomal maintenance of human papillomavirus genomes in primary human keratinocytes. | papillomaviruses possess small dna genomes that encode five early (e) proteins. transient dna replication requires activities of the e1 and e2 proteins and a dna segment containing their binding sites. the e6 and e7 proteins of cancer-associated human papillomavirus (hpv) transform cells in culture. recent reports have shown that e6 and e7 are necessary for episomal maintenance of hpv in primary keratinocytes. the functions of e6 necessary for viral replication have not been determined, and to a ... | 2002 | 12388696 |
| the significance of hyperkeratosis/parakeratosis on otherwise normal papanicolaou smears. | the purpose of this study was to determine whether hyperkeratosis/parakeratosis found on an otherwise normal papanicolaou smear was associated with abnormalities after comprehensive evaluation. | 2002 | 12388995 |
| oncogenic human papillomavirus (hpv) infection and uterine cervical cancer: a screening strategy in the perspective of rural india. | the predominance of cervical cancer in india can mostly be attributed to the lack of early screening. the objective of the present study has been, therefore, to determine a cost-effective oncogenic human papillomavirus (hpv)-based cervical cancer screening plan for rural indian women. the results showed that in normal women, highest prevalence of hpv 16/18 infection was in the age group < or =23 years and lowest in > or =44 years with an insignificant change in between. hpv 16/18 infection was s ... | 2002 | 12394242 |
| p16ink4a immunohistochemistry improves interobserver agreement in the diagnosis of cervical intraepithelial neoplasia. | it has been repeatedly shown that there is a substantial lack of interobserver reproducibility in the histologic diagnosis of cervical intraepithelial neoplasia (cin), which might be improved by a more specific diagnostic biomarker. cervical cancer and cin, but not other cervical epithelia, express high levels of the cyclin-dependent kinase inhibitor p16, suggesting that staining for this marker could help to more precisely identify cin in tissue sections and therefore reduce variation in interp ... | 2002 | 12409715 |
| obtaining sensitive data through the web: an example of design and methods. | several studies have suggested that the quality of coital data from diaries is superior to that collected by retrospective questionnaires. by collecting data over short intervals of time, diaries can present a more comprehensive picture of exposure, while minimizing the potential for recall bias. despite these advantages, paper diaries have limited use because of their expense and difficulty of implementation. web-based data collection offers the opportunity to make improvements to the quality o ... | 2002 | 12410004 |
| absolute risk of a subsequent abnormal pap among oncogenic human papillomavirus dna-positive, cytologically negative women. | the addition of human papillomavirus (hpv) dna testing to cytologic screening for cervical carcinoma is now being considered. the majority of women in screening cohorts who test positive for oncogenic types of hpv dna have concurrent negative pap tests. the absolute risk of a subsequent abnormal pap test for these women is uncertain. therefore, the proper counseling and clinical management of these women is also uncertain. | 2002 | 12412168 |
| amino acid residues in the carboxy-terminal region of cottontail rabbit papillomavirus e6 influence spontaneous regression of cutaneous papillomas. | previous studies have identified two different strains of cottontail rabbit papillomavirus (crpv) that differ by approximately 5% in base pair sequence and that perform quite differently when used to challenge new zealand white (nzw) rabbit skin. one strain caused persistent lesions (progressor strain), and the other induced papillomas that spontaneously regressed (regressor strain) at high frequencies (j. salmon, m. nonnenmacher, s. caze, p. flamant, o. croissant, g. orth, and f. breitburd, j. ... | 2002 | 12414922 |
| the human papillomavirus type 16 e5 protein impairs trail- and fasl-mediated apoptosis in hacat cells by different mechanisms. | the effect of the human papillomavirus type 16 (hpv-16) e5 protein on apoptosis was investigated by using the polyclonal hacat-cell lines stably transfected either with e5 (hacat/e5) or the empty vector (hacat/pmsg) as reference. apoptosis was triggered either by fas ligand (fasl) or by tumor necrosis factor-related apoptosis-inducing ligand (trail) and was monitored by detection of cleavage of procaspase-8 and procaspase-3, as well as their substrate poly(adp-ribose) polymerase (parp). in contr ... | 2002 | 12414956 |
| pathological features of betel quid-related oral epithelial lesions in taiwan with special emphasis on the tumor progression and human papillomavirus association. | betel quid (bq) chewing has been a well-documented cause of oral epithelial lesions (oel). evolution from early hyperplastic lesions to the late or carcinomatous stage has been recognized. the pathobiological and molecular mechanism, however, remains to be elucidated. in this study, a total of 232 samples obtained from 153 cases of bq-related oel were retrospectively evaluated for the expression of p53 and bcl-2 in comparison with 26 cases of bq-unrelated lesions (n = 29). the possible role of h ... | 2002 | 12417791 |
| male circumcision, penile human papillomavirus infection, and cervical cancer. | 2002 | 12418039 | |
| carcinoma cuniculatum of the jaw: a rare variant of oral carcinoma. | carcinoma cuniculatum is a rare variant of carcinoma usually involving the foot. only 13 cases have been described in the oral cavity, none of which was in the english-language literature. we describe a 56-year-old man who presented with a soft exophytic mass in the maxillary gingiva, alveolar bone destruction, and loosening of the teeth. histopathologic study revealed thin papillary projections covered by a thick keratin layer in the superficial areas and multiple, branching keratin-filled cryp ... | 2002 | 12424455 |
| cysteine-modifying agents: a possible approach for effective anticancer and antiviral drugs. | modification of cysteine residues in proteins, due to a) the participation of the thiol moiety of this amino acid in oxido-reduction reactions, b) its ability to strongly coordinate transition metal ions, or c) its nucleophilic nature and facile reaction with electrophiles, may be critically important for the design of novel types of pharmacological agents. application of such procedures recently led to the design of novel antivirals, mainly based on the reaction of zinc finger proteins with dis ... | 2002 | 12426135 |
| use of mature dendritic cells for anti-human papillomavirus peptide-based vaccination in transplant patients. | 2002 | 12431627 | |
| repeated dna vaccinations elicited qualitatively different cytotoxic t lymphocytes and improved protective antitumor effects. | the use of dna vaccines for generating antigen-specific cd8+ t cell responses has been well established. however, little is known about the quantitative and qualitative aspects of cd8+ t cell responses and protective immunity generated after repeated dna vaccinations. we used human papillomavirus (hpv) type-16 e7 as a model tumor antigen in an e7-expressing tumor model, tc-1, to assess the influence of the frequency of dna vaccinations on e7-specific immunological and antitumor responses. mice w ... | 2002 | 12432234 |
| expression of the the cyclin-kinase inhibitors p21(waf1) and p27(kip1) and the p53 tumor suppressor genes in adult-onset laryngeal papillomas. | different types of human papilloma virus are known to be closely associated with laryngeal papillomas. on the other hand, the proliferation of epithelial cells is associated with various abnormalities in the mechanisms of cellular regulation. in this study, we detected the expressions of p53, p21 and p27 proteins in adult-onset laryngeal papillomas by immunohistochemical techniques. the objective of this study is to evaluate the expression of these factors in adult-onset laryngeal papillomas and ... | 2002 | 12434183 |
| identification of a novel promoter in the e2 open reading frame of the human papillomavirus type 18 genome. | northern blot and rt-pcr analyses indicated that the human papillomavirus e4 open reading frame is expressed in hela cells. because integration at the e1 or e2 open reading frame would place the viral upstream regulatory region downstream of the viral late genes, the expression of e4 in hela cells is most likely regulated by host cellular promoter(s) or unidentified viral promoter(s) in the e2 region. primer extension analysis and transient transfection experiments with luciferase reporter const ... | 2003 | 12436488 |
| trachea, lung, and tracheobronchial lymph nodes are the major sites where antigen-presenting cells are detected after nasal vaccination of mice with human papillomavirus type 16 virus-like particles. | vaccination by the nasal route has been successfully used for the induction of immune responses. either the nasal-associated lymphoid tissue (nalt), the bronchus-associated lymphoid tissue, or lung dendritic cells have been mainly involved. following nasal vaccination of mice with human papillomavirus type 16 (hpv16) virus-like-particles (vlps), we have previously shown that interaction of the antigen with the lower respiratory tract was necessary to induce high titers of neutralizing antibodies ... | 2002 | 12438585 |
| do condoms prevent genital hpv infection, external genital warts, or cervical neoplasia? a meta-analysis. | although condoms most likely prevent hiv infection, evidence of their effectiveness against other sexually transmitted diseases is mixed. | 2002 | 12438912 |
| prevalence of genital human papillomavirus among lebanese women. | the purpose of this study was to determine the prevalence of human papillomavirus (hpv), and more specifically of hpv 16, in a group of lebanese women. | 2002 | 12440818 |
| cervical biopsy specimens and human papilloma virus positivity in patients with external genital warts. | cervical biopsy specimens were taken from 16 women with external genital warts in order to diagnose concomitant cervical human papillomavirus (hpv) infection and cervical intraepithelial neoplasia (cin) according to pathology. positive hpv infection was detected 56% (9/16) of the patients. among those patients 66% (6/9) had concurrent subclinical low-grade cin lesions. one of those patients had a high-grade cin lesion. it is concluded that cervical hpv infection was rather common in women with e ... | 2002 | 12440827 |
| [incidence and management of condylomata acuminata by french general physicians]. | condylomata acuminata (or genital warts) are sexually transmitted diseases caused by human papillomavirus. until now, there has been no available epidemiologic data about this disease in france. we conducted a prospective study among french general practitioners (gps) to estimate the incidence of consultations for external condylomata acuminata in general practice. we also assessed the management of patients with external condylomata acuminata by french gp's. | 2002 | 12442095 |