Publications

addition of right lateral decubitus positioning improves vertebral visualization with vfa in selected patients.	densitometric vertebral fracture assessment (vfa) allows detection of clinically unappreciated vertebral fracture. however, vertebral visualization using vfa can be suboptimal. in such individuals, alternative spine positioning may enhance visualization. consistent with this, we observed that reversal of positioning (right lateral decubitus rather than standard left lateral decubitus, subsequently referred to as "reverse" positioning) improved visualization in patients with suboptimal vertebral ...	2006	16931359
the prognostic value of severe malnutrition in the development of nonthyroidal illness in head and neck cancer patients.	thyroid hormone metabolism is modulated by starvation and overfeeding but also by dietary composition. unfortunately, little is known about the effect of malnutrition on disease-induced nonthyroidal illness (nti). in this study, we investigated whether the degree of nti after surgery differed between severely malnourished and well-fed patients with head and neck cancer.	2006	16931610
clinical utility of measurements of insulin-like growth factor 1.	plasma insulin-like growth factor 1 (igf-i) concentrations are regulated by genetic factors, nutrient intake, growth hormone (gh) and other hormones such as t4, cortisol and sex steroids. the accuracy of igf-i measurement in diagnosing gh deficiency or excess depends, in part, on the relative contributions of each of these variables. since their respective influence may vary widely between individuals, the establishment of well-defined normal ranges is necessary, which requires adequate numbers ...	2006	16932333
oxidative stress during 1-lung ventilation.	resuming 2-lung ventilation from 1-lung ventilation might induce a re-expansion and reoxygenation effect. the oxidative stress during 1-lung ventilation/2-lung ventilation has not been studied, although severe complications, such as re-expansion pulmonary edema, were reported. reactive oxygen species production and total antioxidant status assay levels were measured in this study during 1-lung ventilation/2-lung ventilation. the effects on extravascular lung water, cardiac output, and intrathora ...	2006	16935103
effect of l-thyroxine treatment on left ventricular function in subclinical hypothyroidism.	aim of this study was to investigate the effects of thyroxine treatment on myocardial regional left ventricular (lv) systolic and diastolic function in patients with subclinical hypothyroidism (sh) by tissue doppler imaging (tdi). forty-two patients (29 women and 13 men; mean age 52.2+/-15.1 years) with sh, as judged by elevated serum thyroid-stimulating hormone (tsh) levels (>3.6 miu/l; range, 3.8-12.0) and free thyroid hormones (ft4 and ft3) within the normal range, and 30 euthyroid volunteers ...	2006	16935462
regulation of type iii iodothyronine deiodinase expression in human cell lines.	type i iodothyronine deiodinase (d1) and type ii iodothyronine deiodinase (d2) catalyze the activation of the prohormone t4 to the active hormone t3; type iii iodothyronine deiodinase (d3) catalyzes the inactivation of t4 and t3. d3 is highly expressed in brain, placenta, pregnant uterus, and fetal tissues and plays an important role in regulating thyroid hormone bioavailability during fetal development. we examined the activity of the different deiodinases in human cell lines and investigated t ...	2006	16935842
[clinical and muscular evaluation in patients with subclinical hypothyroidism].	some symptoms and signs of hypothyroidism, as well as some laboratory abnormalities, may be present in subclinical hypothyroidism (sh). this study evaluates the prevalence of signs and symptoms of hypothyroidism and skeletal muscle dysfunction in 57 patients with sh compared to 37 euthyroid controls. the participants received a clinical score based on signs and symptoms of hypothyroidism. the muscle strength was estimated by manual testing and chair dynamometer and inspiratory force by manuvacuo ...	2006	16936994
relations among mothers' expressivity, children's effortful control, and their problem behaviors: a four-year longitudinal study.	longitudinal relations between mothers' expressivity, children's effortful control, and their problem behaviors were examined when children (n = 181) were 6.5-10 years old (t2) and again 2 (t3) and 4 (t4) years later. mothers reported on their expression of positive and negative dominant emotion. mothers and teachers reported on children's effortful control and externalizing and internalizing problem behaviors. in structural equation models, variables exhibited consistency over time. further, th ...	2006	16938087
rna chaperone activity of translation initiation factor if1.	translation initiation factor if1 is an indispensable protein for translation in prokaryotes. no clear function has been assigned to this factor so far. in this study we demonstrate an rna chaperone activity of this protein both in vivo and in vitro. the chaperone assays are based on in vivo or in vitro splicing of the group i intron in the thymidylate synthase gene (td) from phage t4 and an in vitro rna annealing assay. if1 wild-type and mutant variants with single amino acid substitutions have ...	2006	16938378
leptin acute modulation of the 5'-deiodinase activities in hypothalamus, pituitary and brown adipose tissue of fed rats.	leptin and thyroid hormones (th) have the ability to increase energy expenditure. biological effects of th are dependent on thyroxine (t4) to triiodothyronine (t3) conversion by deiodinase type 1 (d1) and type 2 (d2). leptin has been shown to stimulate the hypothalamus-pituitary-thyroid axis and, also, to modulate 5'-deiodinases in different tissues, depending on energetic status of animals. here, we examined the acute effects of leptin on hypothalamic, pituitary and bat d2 and pituitary d1 acti ...	2006	16941271
response of four types of coliphages to high hydrostatic pressure.	pressure inactivation of four types of coliphages, varphix 174 (ssdna virus), ms2 (ssrna virus), lambda imm434 (dsdna virus) and t4 (dsdna virus), was studied to evaluate their potential as human enteric viral surrogates for use in validation of commercial pressure processing treatments. phage varphix 174 demonstrated an unexpected high resistance to pressure with no more than 1-log(10) reduction observed following exposures to 350-600 mpa. there was no greater than 1-log(10) reduction below 500 ...	2006	16943050
[tc]-99m thyroid scintigraphy in congenital hypothyroidism screening program.	the aetiology of congenital hypothyroidism (ch) may be important in determining disease severity, outcome and treatment schedules because athyroid patients need higher treatment doses and close monitoring particularly early in life. the aim of this study was to evaluate thyroid scintigraphy (ts) findings in infants with ch and to determine the relationship of serum tsh and t4 values with thyroid agenesia, in an attempt to identify factors that may detect thyroid agenesia before treatment. since ...	2006	16943215
[anterolateral thigh perforator flaps for facial reconstruction after tumour surgery].	individual flap design and minor donor site morbidity are main criteria in the treatment of facial defects after tumour surgery. microvascular perforator flaps seem to follow these criteria well. in the following study our experiences with microvascular anterolateral thigh perforator flaps (altpf) in reconstruction of the face following ablative tumour surgery are described and discussed in comparison to the present literature.	2006	16944084
a biomechanical analysis of sublaminar and subtransverse process fixation using metal wires and polyethylene cables.	an in vitro biomechanical calf thoracic spine study.	2006	16946654
neuromodulatory role of 5-hydroxytryptamine type 1a receptors on the excitability of renshaw cells in the rat spinal cord.	serotonin modulates neurotransmission at synapses between various spinal neurons and, thus, may either facilitate or depress the information processed in the circuits and ultimately modulate the final motor output. a possible neuromodulatory role of 5-hydroxytryptamine type 1a (5-ht1a) receptors on the excitability of lumbar spinal renshaw cells was explored in anesthetized rats spinalized at t4 level. intravenous administration of the specific 5-ht1a agonist (2r)-(+)-8-hydroxy-2-(di-n-propylami ...	2006	16951575
early activation of an altered thyroid hormone profile in asymptomatic or mildly symptomatic idiopathic left ventricular dysfunction.	although an altered thyroid metabolism has been documented in patients with overt heart failure, no evaluation has been made of a heart-thyroid interaction in mildly symptomatic patients with idiopathic left ventricular dysfunction (ilvd). we wanted to assess the thyroid state in patients with ilvd.	2006	16952785
ectopic fascioliasis in the dorsal spine: case report.	parasitic myelopathy is rare. we report ectopic infestation of the spine by fasciola hepatica.	2006	16955027
degradability of dimethyl terephthalate by variovorax paradoxus t4 and sphingomonas yanoikuyae dos01 isolated from deep-ocean sediments.	two strains of bacteria were isolated from deep-ocean sediments of the south china sea using enrichment culturing technique and they were identified as sphingomonas yanoikuyae dos01 (ay878409) and variovorax paradoxus t4 (ay878410) based on 16s rrna gene sequences. s. yanoikuyae dos01 was only capable of transforming dimethyl terephthalate (dmtp) to monomethyl terephthalate (mmtp) without further degradation while v. paradoxus t4 exhibited ability in mineralizing dmtp as the sole source of carbo ...	2006	16955363
[endemic goiter and iodine deficiency: are they still a reality in spain].	to estimate the prevalence of goiter and iodine deficiency in a health district in the autonomous community of valencia, given the absence of data in this region of spain.	2006	16956503
association and dissociation of the cell puncturing complex of bacteriophage t4 is controlled by both ph and temperature.	the tail lysozyme, gp5, of bacteriophage t4 is a trimeric protein and all the subunits are nicked between ser351 and ala352 during assembly through processing. when subsequently heated, the resulting (gp5)(3) (gp5c)(3) (the asterisk "" denotes that the intact pre-gp5 trimer has been nicked) dissociates into three gp5* (three independent n-terminal monomeric peptides, that carry lysozyme moieties at the c-termini of gp5*), and a c-terminal trimeric beta-helical structure (gp5c)(3). the interact ...	2006	16956798
thyroid function and morphology after a successful kidney transplantation.	although thyroid disorders related to the end-stage renal disease (esrd) are well known, there are discordant data on the function and morphology of the thyroid gland after renal transplantation (rt). the objective of this cross-sectional, case-control study was to investigate the prevalence and risk factors for disorders in the thyroid function and morphology after a successful rt. fifty consecutive patients (25 females, 25 males) with fully functioning allograft were enrolled. their age at tra ...	2006	16957411
rifampin-induced hypothyroidism.	three euthyroid patients with hashimoto's thyroiditis developed hypothyroidism after the administration of rifampin. we studied 67 patients with tuberculosis. all of them were treated with rifampin. of the 67 patients, 42 had negative tests for anti-thyroid antibodies (ata) and 25 had positive tests for ata. the diagnosis of hashimoto's thyroiditis was made on the basis of positive tests for ata. after the administration of rifampin, tsh levels were not significantly altered in all of the former ...	2006	16957414
mechanisms of disease: psychomotor retardation and high t3 levels caused by mutations in monocarboxylate transporter 8.	the actions and the metabolism of thyroid hormone are intracellular events that require the transport of iodothyronines across the plasma membrane. it is increasingly clear that this process does not occur by simple diffusion, but is facilitated by transport proteins. only recently have iodothyronine transporters been identified at the molecular level, of which organic anion transporting polypeptide 1c1 and monocarboxylate transporter 8 (mct8) deserve special mention, because of their high activ ...	2006	16957765
tsh-induced hyperthyroidism caused by a pituitary tumor.	a 45-year-old man presented with frontal headache and visual disturbances to our clinic. for the previous 5 years, he had been receiving treatment for long-lasting mild hyperthyroidism with antithyroid therapy, but therapy had not been carefully followed. during the last 2 years he had also complained of erectile dysfunction and loss of libido. on physical examination, he had a small goiter, normal skin, no graves' ophthalmopathy, normal bmi, and reduced testis volume and pubic hair.	2006	16957766
molecular interactions involving escherichia coli nucleoside diphosphate kinase.	nucleoside diphosphate kinase plays a distinctive metabolic role as the enzyme poised between the last reaction of deoxyribonucleoside triphosphate (dntp) biosynthesis and the dna polymerization apparatus. in bacteriophage t4 infection, ndp kinase is one of very few enzymes of host cell origin to participate in either dntp synthesis or dna replication. yet ndp kinase forms specific contacts with phage-coded proteins of dntp and dna synthesis. this article summarizes work from our laboratory that ...	2006	16957984
[investigation of relationship between prehospital care for traumatic shock and postoperative acute lung injury/acute respiratory distress syndrome].	to investigate and analyze the correlation and clinical significance of prehospital treatment for traumatic shock and postoperative acute lung injury (ali)/acute respiratory distress syndrome (ards).	2006	16959149
esophageal cancer: determination of internal target volume for conformal radiotherapy.	to evaluate esophageal tumor and oar movement during the respiratory cycle in order to obtain optimal values for itv and prv. to correlate tumor motion with chest wall displacement - information of value in the free-breathing gating system.	2006	16959345
developmental control of iodothyronine deiodinases by cortisol in the ovine fetus and placenta near term.	preterm infants have low serum t4 and t3 levels, which may partly explain the immaturity of their tissues. deiodinase enzymes are important in determining the bioavailability of thyroid hormones: deiodinases d1 and d2 convert t4 to t3, whereas deiodinase d3 inactivates t3 and produces rt3 from t4. in human and ovine fetuses, plasma t3 rises near term in association with the prepartum cortisol surge. this study investigated the developmental effects of cortisol and t3 on tissue deiodinases and pl ...	2006	16959839
in vitro culture, serologic and molecular analysis of acanthamoeba isolated from the liver of a keel-billed toucan (ramphastos sulfuratus).	members of the genus acanthamoeba are usually free-living amebae and are found in a variety of ecological niches including soil, fresh and brackish water, dust in air, filters of heating, ventilating, and air conditioning units, swimming pools and hot tubs, etc. occasionally, they are also known to cause central nervous system infections in humans and other animals. we isolated into culture an amoeba from the liver tissue of a keel-billed toucan and identified it as acanthamoeba sp. based on cul ...	2007	16962705
[inhibition of integrin beta1 expression in human keratinocyte stem cells by vector-1 based interfering rna strategy].	to investigate the influence of integrin beta1 on the proliferation and differentiation of human keratinocyte stem cells (kscs).	2006	16964653
analysis of physical and mental development of children with aplasia, hypoplasia and ectopy of the thyroid gland.	evaluation of clinical and biochemical differences between various forms of thyroid dysgenesia in children.	2006	16964962
safety, tolerance, and metabolism of broccoli sprout glucosinolates and isothiocyanates: a clinical phase i study.	broccoli sprouts are widely consumed in many parts of the world. there have been no reported concerns with respect to their tolerance and safety in humans. a formal phase i study of safety, tolerance, and pharmacokinetics appeared justified because these sprouts are being used as vehicles for the delivery of the glucosinolate glucoraphanin and its cognate isothiocyanate sulforaphane [1-isothiocyanato-(4r)-(methylsulfinyl)butane] in clinical trials. such trials have been designed to evaluate prot ...	2006	16965241
treatment results of 165 pediatric patients with non-metastatic nasopharyngeal carcinoma: a rare cancer network study.	this rare cancer network (rcn) study was performed in pediatric nasopharyngeal carcinoma (pnpc) patients to evaluate the optimal dose of radiotherapy and to determine prognostic factors.	2006	16965827
long-term outcome of patients treated by radiation therapy alone for salivary gland carcinomas.	to review a single-institution experience with the management of salivary gland cancers treated by radiation alone.	2006	16965870
[interhemisphere differences of extrastriate cortical activation during attention and selection of lateralized visual stimuli in humans].	the cortical activation was estimated by event-related potentials (erps) methods during selection tasks of lateralized visual stimuli in right and left hemi-fields requiring different forms of attention: 1. attention of a stimuli form, 2. attention of a stimuli position, 3. combined attention of form and position. erps were recorded in 15 young healthy adults in 6 leads: p3, p4, t3, t4, t5, t6 and endogenous erps components: cnv (contingent negative variation), n1, p3 and complex [n1--p3]. the d ...	2006	16967868
transfer of surface polymerase reaction products to a secondary platform with conservation of spatial registration.	here, we describe a method for directly transferring very small amounts of reaction products from one surface to another. the approach is illustrated using a t4 dna polymerase reaction to extend primers hybridized to a surface-confined dna template. following the extension reaction, the resulting oligonucleotide is transferred to a product surface. the important results are that (1) the spatial registration of the product is preserved after transfer; (2) the same reactant surface can be used to ...	2006	16967951
atomically detailed simulations of concentrated protein solutions: the effects of salt, ph, point mutations, and protein concentration in simulations of 1000-molecule systems.	an ability to accurately simulate the dynamic behavior of concentrated macromolecular solutions would be of considerable utility in studies of a wide range of biological systems. with this goal in mind, a brownian dynamics (bd) simulation method is reported here that allows systems to be modeled that comprise in excess of 1000 protein molecules, all of which are treated in atomic detail. intermolecular forces are described in the method using an energy function that incorporates electrostatic an ...	2006	16967959
far field thermal radiation through nanoholes and apertures.	thermal radiation through a nanohole or a nanoaperture is calculated in this paper. results show that for aperture size << dominant wavelength of thermally excited photons, heat flux deviates significantly from the well-known planck blackbody expression of sigmat4, where sigma is the stefan-boltzmann constant and t is the temperature. the heat flux through a nanohole is proportional to t8 and a4, where a is the radius of the hole. finally it is shown that for at < 10(6), where a is expressed in ...	2006	16968039
hormonal change and cytokine mrna expression in peripheral blood mononuclear cells during the development of canine autoimmune thyroiditis.	to elucidate the hormonal change and alteration in cytokine expression in peripheral blood mononuclear cells (pbmc) during the early stage of autoimmune thyroiditis, we have developed a canine model of this disease, in which normal dogs were immunized with bovine thyroglobulin (tg) and/or canine thyroid extract. serum samples were collected weekly, anti-canine tg antibody was measured by enzyme-linked immunosorbent assay (elisa) and thyroid stimulating hormone (tsh) and total t4 levels by radioi ...	2006	16968404
influences of age, gender, smoking, and family history on autoimmune thyroid disease phenotype.	both genetic and environmental factors contribute to susceptibility to graves' disease (gd) and hashimoto's thyroiditis (ht), as well as disease manifestations.	2006	16968788
positron emission tomography reveals correlations between brain metabolism and mood changes in hyperthyroidism.	hyperthyroidism is frequently associated with emotional distress. the underlying cerebral processes of the endocrine-induced mood changes are unclear.	2006	16968792
[long-term results of chemoradiation therapy for the patients with locally advanced (t4) esophageal cancer].	the prognosis of patients with advanced esophageal cancer is still poor. recently, concurrent chemoradiation therapy for esophageal cancer is being utilized with increasing frequency. in this study, we reported concurrent chemoradiation for patients with t4 esophageal cancer. from july 2000, we treated 21 consecutive patients with radiation and concurrent chemotherapy using intermittent low-dose fp chemoradiation (40 gy radiation, 2 gy/day, for 4 weeks 280/m(2) 5-fu intermittent 24 continuous, c ...	2006	16969020
spreeta-based biosensor assays for endocrine disruptors.	the construction and performance of an automated low-cost spreeta-based prototype biosensor system for the detection of endocrine disrupting chemicals (edcs) is described. the system consists primarily of a spreeta miniature liquid sensor incorporated into an aluminum flow cell holder, dedicated to support a biacore chip frame, in combination with a simple pressurized air-driven fluid system. during the optimization, a monoclonal antibody (mab)-based immunoassay for the estrogenic compound bisph ...	2007	16971108
induction chemotherapy, concurrent chemoradiation and surgery for pancoast tumour.	the traditional treatment of pancoast tumour with local approaches (surgery, radiotherapy or a combination of both) leads to a poor outcome due to the high rate of incomplete resection and the lack of systemic control. the aim of the present prospective feasibility study was to determine whether a trimodality approach improves local control and survival. patients with stage iib-iiib pancoast tumour received induction chemotherapy (three courses of split-dose cisplatin and etoposide or paclitaxel ...	2007	16971407
biochemical analysis of the substrate specificity and sequence preference of endonuclease iv from bacteriophage t4, a dc-specific endonuclease implicated in restriction of dc-substituted t4 dna synthesis.	endonuclease iv encoded by denb of bacteriophage t4 is implicated in restriction of deoxycytidine (dc)-containing dna in the host escherichia coli. the enzyme was synthesized with the use of a wheat germ cell-free protein synthesis system, given a lethal effect of its expression in e.coli cells, and was purified to homogeneity. the purified enzyme showed high activity with single-stranded (ss) dna and denatured dc-substituted t4 genomic double-stranded (ds) dna but exhibited no activity with dsd ...	2006	16971463
chemotherapy with preoperative radiotherapy in rectal cancer.	preoperative radiotherapy is recommended for selected patients with rectal cancer. we evaluated the addition of chemotherapy to preoperative radiotherapy and the use of postoperative chemotherapy in the treatment of rectal cancer.	2006	16971718
serotonin potentiates sympathetic responses evoked by spinal nmda.	in urethane-chloralose anaesthetized, neuromuscularly blocked, ventilated rats, we examined the effects on sympathetic outflow to brown adipose tissue (bat) of separate and simultaneous spinal microinjections of nmda and serotonin. microinjection of nmda (12 pmol) into the right t4 spinal intermediolateral nucleus (iml) immediately increased ipsilateral brown adipose tissue (bat) sympathetic nerve activity (sna; peak: +546% of control), bat thermogenesis (+0.8 degrees c) and heart rate (+53 beat ...	2006	16973701
cardiovascular and temperature changes in spinal cord injured rats at rest and during autonomic dysreflexia.	in patients with high spinal cord injuries autonomic dysfunction can be dangerous, leading to medical complications such as postural hypotension, autonomic dysreflexia and temperature disturbance. while animal models have been developed to study autonomic dysreflexia, associated temperature changes have not been documented. our aim here was to use radiotelemetry and infrared thermography in rodents to record the development of cardiovascular and skin temperature changes following complete t4 tra ...	2006	16973703
unexpected peripheral markers of thyroid function in a patient with a novel mutation of the mct8 thyroid hormone transporter gene.	the specific thyroid hormone transporter, mct8, located on the x chromosome, has led to the identification a novel syndrome. the objective is to relate phenotype with several tissue-specific thyroid functions. a 1-year-old boy, who had severe psychological damage and low serum t4, had received l-t4 for 3 months. at admission, body length was normal but weight was low. off therapy, serum tsh was mildly elevated, serum t4 and free t4 were low, and serum t3 and free t3 were high. direct sequencing ...	2007	16974106
increasing expression of fascin in renal cell carcinoma associated with clinicopathological parameters of aggressiveness.	to determine whether higher expression of fascin, an actin-bundling protein associated with motility, in conventional renal cell carcinoma (rcc) is associated with more advanced stages of the disease.	2006	16977579
responses of thyroid hormones of market-size broilers to thermoneutral constant and warm cyclic temperatures.	this study characterizes the responses, particularly diurnal variations, of thyroid hormones (th) [3,5,3'-triiodothyronine (t3), thyroxine (t4), and t3:t4 ratio (t3/t4)] of arbor acres broilers to constant thermoneutral (tn) or warm cyclic (wc) temperatures. there existed distinct circadian variations--2 peaks in th under the tn and wc conditions. the 2 peaks of t3, t4, and t3/t4 of the broilers occurred, respectively, at 0 and 16 h, 8 and 16 h, and 0 and 12 h under the tn condition; but at 0 an ...	2006	16977836
characterization and gene expression profiling of five new human embryonic stem cell lines derived in taiwan.	many human embryonic stem cell (hesc) lines have been reported, but only a few of them have been fully characterized. in this report, five new hesc lines were derived from 32 discarded blastocysts in taiwan, and these lines were continuously cultured on mitotically inactivated mouse embryonic fibroblast (mef) feeder layer in the hesc medium for more than 44 passages and underwent freezing/thawing processes. all five hesc lines expressed characteristic undifferentiated hesc markers, such as ssea- ...	2006	16978057
increasing emmprin and matriptase expression in hepatocellular carcinoma: tissue microarray analysis of immunohistochemical scores with clinicopathological parameters.	to examine the expression of extracellular matrix metalloprotease inducer (emmprin) and matriptase in hepatocellular carcinoma (hcc) and to correlate this with tumour progression.	2006	16978202
pyrolysis-field ionization mass spectrometry of rhizodeposits - a new approach to identify potential effects of genetically modified plants on soil organisms.	the objectives of the present study were (1) to investigate the qualitative composition of rhizodeposits leached from soils cropped with non-transgenic and genetically modified (gm) potatoes, and disclose if there were gm-specific modifications in potato rhizodeposition, and (2) to compare these results with conventional bulk parameters of microbial activity in soil. we have raised potatoes from a non-transgenic line (solanum tuberosum l. cv. désirée) and three gm lines, which expressed a gene f ...	2006	16978573
genomic-independent action of thyroid hormones on ntpdase activities in sertoli cell cultures from congenital hypothyroid rats.	the sertoli cells play an essential role in the maintenance and control of spermatogenesis. the ecto-nucleoside triphosphate diphosphohydrolase (e-ntpdase) and 5'-nucleotidase activities can modulate the extracellular adenine nucleotide levels, controlling nucleotide-mediated signaling events in sertoli cells. since thyroid hormones (th) and adenine nucleotides and nucleosides play important modulatory roles in sertoli cell proliferation and differentiation, the aim of our study was to investiga ...	2006	16978660
phase i trial of cetuximab in combination with capecitabine, weekly irinotecan, and radiotherapy as neoadjuvant therapy for rectal cancer.	to establish the feasibility and efficacy of chemotherapy with capecitabine, weekly irinotecan, cetuximab, and pelvic radiotherapy for patients with locally advanced rectal cancer.	2006	16979839
population-based analyses of lymph node metastases in colorectal cancer.	the potential presence of lymph node metastases in patients with colorectal cancer (crc) limits the application of minimally invasive techniques of resection. we used data from the national cancer institute's surveillance, epidemiology, and end results registry to better understand the underlying risk of lymph node metastases and factors that influence this risk in a population-based fashion.	2006	16979956
unfavorable pathological characteristics in familial colorectal cancer with low-level microsatellite instability.	a high degree of microsatellite instability (msi) in colorectal cancer (crc) is a hallmark of hereditary non-polyposis colorectal cancer (hnpcc), caused by germline defects in the mismatch repair (mmr) genes. a low degree of instability (less than 30% of the microsatellites) is seen in a subset of tumors. to clarify the significance of this low degree of msi phenotype, we studied the differences between patients with colorectal tumors with high-level, low-level and no msi. colorectal tumors with ...	2006	16980941
prolactin, cortisol secretion and thyroid function in patients with stroke of mild severity.	different attempts were made to identify the variables that may be involved in the clinical course of cerebrovascular ischemia. in the case of stroke with mild severity (sms), the clinical significance of neuroendocrine changes as well as of post-stroke depression (psd) remains unknown. we therefore evaluated the presence of neuroendocrine changes in the acute and post-acute phase of sms, and their potential role during convalescence. serum cortisol, t4, t3, ft4, ft3, tsh and prl levels were mea ...	2006	16981141
importance of individual side chains for the stability of a protein fold: computational alanine scanning of the insulin monomer.	a new computational approach is proposed to probe the importance of residue side chains for the stability of a protein fold. computational mutations to estimate protein stability (cmeps) is based on the notion that the binding free energy corresponding to the complexation of a given side chain, considered as a "pseudo-ligand" of the wild type protein, reflects the importance of this side chain to the thermodynamic stability of the protein. the contribution of a particular side chain to the foldi ...	2006	16981237
bacteriophage t4 capsid: a unique platform for efficient surface assembly of macromolecular complexes.	we report the first description of a macromolecular complex display system using bacteriophage t4. decorated with two dispensable outer capsid proteins, hoc (155 copies) and soc (810 copies), the 120 nm x 86 nm t4 capsid particle offers a unique binding site-rich platform for surface assembly of hetero-oligomeric complexes. to display the 710 kda anthrax toxin complex, two bipartite functional fusion proteins, lf-hoc and lfn-soc, were constructed. using a defined in vitro binding system, sequent ...	2006	16982068
effects of perinatal exposure to low doses of cadmium or methylmercury on thyroid hormone metabolism in metallothionein-deficient mouse neonates.	perinatal exposure to cadmium (cd) or methylmercury (mehg) results in impaired neurodevelopment. thyroid hormone is essential for normal brain development. however, the issue whether cd or mehg, especially at low doses, interrupts thyroid hormone action remains to be investigated. in the present study, effects of perinatal exposure to low levels of cd or mehg on thyroid hormone metabolism were examined using metallothionein i and ii (mt-i/ii) null or wild-type neonatal mice. dams were exposed to ...	2006	16982123
the serum triiodothyronine to thyroxine (t3/t4) ratio in various thyroid disorders and after levothyroxine replacement therapy.	in order to examine the significance of differences in the triiodothyronine/thyroxine (t3/t4) ratio in the achievement of euthyroidism and in different thyroidal diseases, we studied 1050 subjects: 233 were euthyroid (eu), 239 hypothyroid (hypo) with initial tsh levels >15 mu/l, 273 hypothyroid on substitution therapy with l-thyroxine alone and tsh values of 0.35-3.5 mu/l, (hyporx), 236 hyperthyroid (hyper) and 69 in the acute phase of subacute thyroiditis de quervain's (dq). the ratio of t3/t4 ...	2004	16982586
prospective study of [18f]fluorodeoxyglucose positron emission tomography and computed tomography and magnetic resonance imaging in oral cavity squamous cell carcinoma with palpably negative neck.	to assess the clinical usefulness of [(18)f]fluorodeoxyglucose positron emission tomography ([18f]fdg pet) as well as computed tomography (ct) or magnetic resonance imaging (mri) in oral squamous cell carcinoma (scc) patients with palpably negative neck.	2006	16983105
an rna ligase-mediated method for the efficient creation of large, synthetic rnas.	rna ligation has been a powerful tool for incorporation of cross-linkers and nonnatural nucleotides into internal positions of rna molecules. the most widely used method for template-directed rna ligation uses dna ligase and a dna splint. while this method has been used successfully for many years, it suffers from a number of drawbacks, principally, slow and inefficient product formation and slow product release, resulting in a requirement for large quantities of enzyme. we describe an alternati ...	2006	16983143
effect of methimazole treatment for 2 years on circulating il-4, ige, tbii, and tsab in patients with hyperthyroid graves' disease.	in this study we confirmed our previous findings on the importance of ige in graves' disease and further investigated the relationships existing among graves' disease, ige, and interleukin-4. two hundred and thirty-two newly diagnosed graves' disease patients were treated with methimazole for 2 years, and were classified into 3 groups according to their response to the therapy. incidence of ige elevation (ige> or =170 iu/ml) before treatment was lowest, 23.8%, in the group who achieved remission ...	2006	16983180
long-term results of a randomized trial comparing preoperative short-course radiotherapy with preoperative conventionally fractionated chemoradiation for rectal cancer.	neoadjuvant chemoradiotherapy does not alter anal sphincter preservation or postoperative complications compared with short-course radiotherapy alone in patients with clinical stage t3 or t4 resectable rectal cancer. the aim of this study was to compare survival, local control and late toxicity in the two treatment groups.	2006	16983741
management of amiodarone-induced thyrotoxicosis in latin america: an electronic survey.	to assess diagnostic and therapeutic approaches to amiodarone-induced thyrotoxicosis (ait) among members of the latin american thyroid society (lats).	2006	16984234
searching for interpretable rules for disease mutations: a simulated annealing bump hunting strategy.	understanding how amino acid substitutions affect protein functions is critical for the study of proteins and their implications in diseases. although methods have been developed for predicting potential effects of amino acid substitutions using sequence, three-dimensional structural, and evolutionary properties of proteins, the applications are limited by the complication of the features and the availability of protein structural information. another limitation is that the prediction results ar ...	2006	16984653
dna unwinding by escherichia coli dna helicase i (trai) provides evidence for a processive monomeric molecular motor.	the f plasmid trai protein (dna helicase i) plays an essential role in conjugative dna transfer as both a transesterase and a helicase. previous work has shown that the 192-kda trai protein is a highly processive helicase, catalytically separating >850 bp under steady-state conditions. in this report, we examine the kinetic mechanism describing dna unwinding of trai. the kinetic step size of trai was measured under both single turnover and pre-steady-state conditions. the resulting kinetic step- ...	2006	16984922
a 28-day oral dose toxicity study enhanced to detect endocrine effects of hexabromocyclododecane in wistar rats.	a 28-day repeated dose study in rats (oecd407) enhanced for endocrine and immune parameters was performed with hexabromocyclododecane (hbcd). rats were exposed by daily gavage to hbcd dissolved in corn oil in 8 dose groups with doses ranging between 0 and 200 mg/kg bw per day (mkd). evaluation consisted of dose-response analysis with calculation of a benchmark dose at the lower 95% one-sided confidence bound (bmdl) at predefined critical effect sizes (cess) of 10-20%. the most remarkable finding ...	2006	16984958
analysis of preexistent vertebral rotation in the normal quadruped spine.	in this ct study, vertebral rotation was analyzed in the transverse plane of the normal, nonscoliotic canine spine with a computer-based measurement method.	2006	16985444
lymph node dissection in patients with kidney cancer: when is it indicated?	it is necessary to consider the potential risks and benefits of performing a lymph node dissection (lnd) at the time of radical nephrectomy. lnd may lead to more accurate staging, a decrease in local recurrence, and an increase in survival for patients with metastatic disease limited to the resected lymph nodes. however, there are risks associated with the treatment that must be considered. the advantages of lnd vary among patients, depending on the location and extent of disease progression. fo ...	2003	16985641
chemo-radiation in advanced nasopharyngeal carcinoma, disease free after 6 years--a case report.	this is a case report of a patient with advanced nasopharyngeal carcinoma, (t4 n2 mo) who had chemo-radiation with cisplatin based chemotherapy and total midplane dose of 60 gray external beam radiation. six years after treatment patient has remained disease free and the primary site histologically confirmed disease free with no clinical evidence of regional or distance metastases	2006	16986298
agreement between picco pulse-contour analysis, pulmonal artery thermodilution and transthoracic thermodilution during off-pump coronary artery by-pass surgery.	haemodynamic instability during off-pump coronary artery bypass surgery (opcab) may appear rapidly, and continuous monitoring of the cardiac index (ci) during the procedure is advisable. with the picco monitor, ci can be measured continuously and almost real time with pulse-contour analysis and intermittently with transthoracic thermodilution. the agreement between pulmonal artery thermodilution ci (tpa), transthoracic thermodilution ci (tpc) and pulse-contour ci (pcci) during opcab surgery has ...	2006	16987335
the dna translocating atpase of bacteriophage t4 packaging motor.	in double-stranded dna bacteriophages the viral dna is translocated into an empty prohead shell by a powerful atp-driven motor assembled at the unique portal vertex. terminases consisting of two to three packaging-related atpase sites are central to the packaging mechanism. but the nature of the key translocating atpase, stoichiometry of packaging motor, and basic mechanism of dna encapsidation are poorly understood. a defined phage t4 packaging system consisting of only two components, proheads ...	2006	16987527
sunflower (helianthus annuus l.).	sunflower (helianthus annuus l.) is considered one of the recalcitrant species in terms of transformation and regeneration. a routine transformation system of this crop requires competent cell cultures for efficient plant regeneration as well as an effective method for gene delivery. a transformation system was developed by an agrobacterium tumefaciens-mediated method using split mature embryonic axis explants from the ha89 genotype. mean transformation efficiency obtained (measured as pcr+ plan ...	2006	16988353
[the histo-clinical analysis of 95 malignant neoplasms of parotid gland].	malignant tumors constituted 25% of all gland tumors, the most frequently in parotid gland.	2006	16989441
mutational analysis of sigma70 region 4 needed for appropriation by the bacteriophage t4 transcription factors asia and mota.	transcriptional activation of bacteriophage t4 middle promoters requires sigma70-containing escherichia coli rna polymerase, the t4 activator mota, and the t4 co-activator asia. t4 middle promoters contain the sigma70 -10 dna element. however, these promoters lack the sigma70 -35 element, having instead a mota box centered at -30, which is bound by mota. previous work has indicated that asia and mota interact with region 4 of sigma70, the c-terminal portion that normally contacts -35 dna and the ...	2006	16996538
epidural spinal cavernous hemangioma.	a 28-year-old woman presented with a rare primary epidural hemangioma without adjacent vertebral involvement manifesting as progressive paraparesis. magnetic resonance imaging of the thoracic spine showed an epidural lesion at t4-5 space appearing as isointense on t1-weighted images with enhancement by contrast medium and hyperintense on t2-weighted images. the lesion was totally removed microsurgically. histological examination revealed cavernous hemangioma. she made a complete recovery from he ...	2006	16998281
long term excessive zn-supplementation promotes metabolic syndrome-x in wistar rats fed sucrose and fat rich semisynthetic diet.	during the last two decades zinc (zn) as a micronutrient is being used indiscriminately in agricultural and husbandry practices and also in baby foods and multivitamin supplements with a view that zn is non-toxic and promotes linear growth and body weight in the consumers. the long-term effect of increasing zn load in the body has not been worked out so far. in this study, three groups of rats were fed on a semi-synthetic diet containing 20 mg (control, group-i), 40 mg (group-ii) and 80 mg zn /k ...	2006	16999025
[multimodal treatment in locally advanced esophageal cancer: a multicenter phase ii study with neoadjuvant oxaliplatin, 5-fluorouracil, leucovorin and neoadjuvant radiotherapy: preliminary results].	the aim of the study was to evaluate the feasibility and efficacy of treatment with oxaliplatin, 5-fluorouracil and leucovorin concomitant with radiation therapy in locally advanced oesophageal cancer. fifty-eight patients with previously untreated, histologically proven oesophageal cancer were enrolled. downstaged patients underwent either surgery or received brachytherapy. we report the results obtained in the first 54 patients. the median age was 58 years. the diagnosis was squamous cell carc ...	2006	16999147
long-term follow-up of orthodontically treated deep bite patients.	the aim of this study was to evaluate the long-term stability of corrected deep bite and mandibular anterior crowding in a sample of 62 subjects (30 patients and 32 controls). the patients began treatment at a mean age of 12.2 years (sd 1.56). the treatment consisted of non-extraction and fixed appliances in 23 subjects and functional appliances in seven. the treatment group was compared with the control group with normal molar occlusion, normal overjet and overbite, no crowding, and without an ...	2006	17000717
effect of levothyroxine on total lipid profiles as assessed by analytical capillary isotachophoresis in a patient with hypothyroidism.	the patient was a 51-year-old japanese female who had been diagnosed with hyperlipidemia. at the first medical examination, her serum levels of total cholesterol (tc) and triglyceride (tg) were 482 and 205 mg/dl, respectively. since hyperlipidemia was not improved by pravastatin, atorvastatin or niceritrol, and since the levels of thyroid-stimulating hormone (tsh) and free t4 were 730 iu/ml and 0.3 ng/dl, respectively, the patient was diagnosed as secondary hyperlipidemia with hypothyroidism. a ...	2006	17001111
transient congenital hypothyroidism due to maternal autoimmune thyroid disease.	the neonatal screening program for congenital hypothyroidism (ch) in greece shows an overall incidence of the disease of 1:2321. the cases with permanent ch have an incidence of 1:2542, whereas the transient forms of ch account for 8.7% of all cases diagnosed as ch. transplacental passage of maternal thyrotropin receptor-blocking antibodies is a rare cause of transient ch. in our program, a retrospective analysis of 508,358 screened newborns revealed 6 infants with transient ch caused by materna ...	2003	17003010
effect of restoration of euthyroidism on peripheral blood cells and erythropoietin in women with subclinical hypothyroidism.	in overt hypothyroidism (oh) anemia is common, while less frequently basophilia has been described. in subclinical hypothyroidism (sch), however, data on the distribution of peripheral blood cells are lacking. therefore, we evaluated the effects of l-t4 replacement therapy on peripheral blood elements in female patients with sch before and after restoration of euthyroidism in a randomized, double-blind and placebo-controlled study.	2003	17003028
prevalence of subclinical thyroid dysfunction and its relation to socioeconomic deprivation in the elderly: a community-based cross-sectional survey.	population-based screening has been advocated for subclinical thyroid dysfunction in the elderly because the disorder is perceived to be common, and health benefits may be accrued by detection and treatment.	2006	17003083
circulating hormones and hypothalamic energy balance: regulatory gene expression in the lou/c and wistar rats.	to ascertain the mechanisms underlying low caloric intake and low body weight in the lou/c rat, the circulating hormone levels and gene expression of hypothalamic peptides and receptors important in energy balance and the induction of suppressor of cytokine signalling 3 (socs3) gene expression in response to leptin challenge were compared with wistar rats. plasma leptin levels were lower in the lou/c rat, as were levels of rat corticosterone, tsh and t4 but not t3. ghrelin levels were higher in ...	2006	17003258
neglected squamous cell carcinoma of the frontal area: a clinical report.	neglected skin carcinomas are not very often in our days. it is known that skin is the most common site of malignancy squamous cell carcinoma (scc) is the second most common malignancy of the skin. we report a combined surgical treatment in an unusual case of a squamous cell carcinoma (t4 n0 m0) localized on scalp, presented primarily as a frontal ulcerative bloody mass, with no palpable lymph nodes and a whole body negative computed tomography. three months after the first surgical procedure, t ...	2006	17003637
subtotal laryngectomy with tracheohyoidopexy: a possible alternative to total laryngectomy.	the objective of this study was to describe a new subtotal laryngectomy technique that foresees two variations: 1) tracheohyoidopexy (thip + a or a-a)-subtotal removal of the larynx maintaining one or two cricoarytenoid units and subsequent tracheohyoidopexy; and 2) tracheohyoidoepiglottopexy (thiep + a or a-a)-resection guarantees preservation of the suprahyoid epiglottis maintaining one or two cricoarytenoid units and further suspension of the tracheal stump at the epiglottis and hyoid bone.	2006	17003702
professional delay in head and neck cancer patients: analysis of the diagnostic pathway.	the aim of this study was to identify which factors are related to specialist delay and to determine the length of the diagnostic pathway in head and neck cancer patients. three hundred and six patients with a carcinoma of the larynx, pharynx or oral cavity were included in the study. logistic regression analysis was used to identify risk factors for specialist delay. large (t3-t4) tumors showed significantly less specialist delay than small (t1-t2) tumors (p=0.045, odds ratio [or]=0.6). pharyng ...	2007	17005438
stability of benefits of mime therapy in sequelae of facial nerve paresis during a 1-year period.	to assess the stability of benefits of mime therapy, a modality of physiotherapy for patients with facial nerve paresis, during a period of 1 year.	2006	17006356
expressions of polypeptide: n-acetylgalactosaminyltransferase in leukemia cell lines during 1,25-dihydroxyvitamin d3 induced differentiation.	the effect of 1,25-dihydroxyvitamin d3 [1,25(oh)(2)d3] on two leukemia cell lines, k562 and shi-1, and its relation to the expression of different subtypes of polypeptide: n-acetylgalactosaminyltransferase (pp-galnac-t) was studied. with morphological and cell flow-cytometric method, it was found that 1,25(oh)(2)d3 induced the differentiation of both leukemia cell lines toward monocytic lineage, but not affected the cell growth and apoptosis. the expressions of different subtypes of pp-galnac-t, ...	2006	17006648
[the effects of amiodaron on the thyroid function].	the use of several groups of medications may result in thyroid dysfunction including thyrotoxicosis or hypothyroidism of various degree (from subclinical to full-clinical syndrome). the mentioned disturbances may develop either on the basis of normal euthyroid gland or may overlap the previously-existing oceult changes (first of all different forms of autoimmune thyroiditis). amiodarone is a widely used anti-arrythmic drug with considerable potential to cause thyroid dysfunction because of its 3 ...	2006	17007301
thyroid function in pulmonary tuberculosis.	to evaluate the thyroid hormone economy in pulmonary tuberculosis (ptb).	2006	17007749
hormonal, auxological and clinical follow-up in children with connatal hiv infection. personal records.	hiv infection and antiretroviral drugs have relevant endocrine implications, affecting growth and pubertal development. moreover stature impairment cannot depend only on decreased hormonal secretion.	2006	17008857
treatment for acute lymphoblastic leukemia in children is associated with papillary carcinoma of thyroid, but not with thyroid disfunction.	the treatment of acute lymphoblastic leukemia (all) in children may cause sequelae, some appearing only at long-term follow-up. we investigated the thyroid gland morphology and the function of the pituitary-thyroid axis in a group of patients treated for all in childhood.	2006	17008858
reg i expression predicts long-term survival among locally advanced thoracic squamous cell esophageal cancer patients treated with neoadjuvant chemoradiotherapy followed by esophagectomy.	the prognosis for patients with locally advanced thoracic esophageal cancer is extremely unfavorable. we have been administering neoadjuvant chemoradiotherapy (crt) followed by esophagectomy to these patients and studying whether reg i expression in untreated endoscopic biopsy specimens is predictive of patient responsiveness to crt and/or survival after treatment.	2006	17009160
synthesis of bisphosphonate derivatives of atp by t4 rna ligase.	t4 rna ligase catalyzes the synthesis of atp beta,gamma-bisphosphonate analogues, using the following substrates with the relative velocity rates indicated between brackets: methylenebisphosphonate (pch(2)p) (100), clodronate (pccl(2)p) (52), and etidronate (pc(oh)(ch(3))p) (4). the presence of pyrophosphatase about doubled the rate of these syntheses. pamidronate (pc(oh)(ch(2)-ch(2)-nh(2))p), and alendronate (pc(oh)(ch(2)-ch(2)-ch(2)-nh(2))p) were not substrates of the reaction. clodronate disp ...	2006	17010342
tumor characteristics and prognostic factors in two subsequent screening rounds with four-year interval within prostate cancer screening trial, erspc rotterdam.	to evaluate the tumor characteristics and prognostic factors in screen-detected prostate cancers in two successive screening rounds with a 4-year screening interval in the european randomized study of screening for prostate cancer, section rotterdam.	2006	17010732