Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| reverse transcription-polymerase chain reaction amplification of respiratory syncytial virus genome from neonatal nasal swab samples. | in order to make a rapid and accurate diagnosis of respiratory syncytial virus (rsv) infection, nasal swabs obtained from 14 neonates suspected of having this disease were examined for the presence of rsv genome by reverse transcription (rt) and nested polymerase chain reaction (pcr) amplification, along with enzyme immunoassay (eia), serum neutralization testing and virus isolation. the rt-pcr method was sensitive enough to detect a 0.1 50% tissue culture infectious dose (tcid50) per milliliter ... | 1996 | 8941998 |
| solution structure of the immunodominant region of protein g of bovine respiratory syncytial virus. | the three-dimensional solution structure of the immunodominant central conserved region of the attachment protein g (brsv-g) of bovine respiratory syncytial virus has been determined by nuclear magnetic resonance (nmr) spectroscopy. in the 32-residue peptide studied, 19 residues form a small rigid core composed of two short helices, connected by a type i' turn, and linked by two disulfide bridges. this unique fold is among the smallest stable tertiary structures known and could therefore serve a ... | 1996 | 8942628 |
| respiratory viruses in a hospitalized paediatric population in edinburgh 1985-1994. | we describe the incidence of respiratory viruses identified in children admitted to an edinburgh hospital between october 1985 and july 1994. respiratory syncytial (rs) virus, influenza viruses and parainfluenza viruses showed seasonal activity whereas adenoviruses and rhinoviruses did not. parainfluenza viruses were the most changeable in their epidemiological behaviour and rs virus the least. | 1996 | 8945711 |
| a 6-week-old girl with persistent apnea and worsening pulmonary disease. | 1996 | 8946125 | |
| [value of surfactant in the treatment of acute respiratory distress syndrome in a child]. | adult respiratory distress syndrome has a high mortality rate, despite treatment including mechanical ventilation with positive end-expiratory pressure, increase of the inspiratory time and high inspiratory oxygen concentration. exogenous surfactant, a well established treatment in premature newborns with neonatal respiratory distress syndrome has only been occasionally evaluated in adult respiratory distress syndrome. | 1996 | 8949353 |
| serological study of responses to selected pathogens causing respiratory tract infection in the institutionalized elderly. | in a prospective 2-year study, serological responses to selected pathogens were analyzed in 224 episodes of fever attributable to respiratory tract infection (51.8%) or of unknown source (48.2%) in 131 residents of two long-term-care facilities. a serological response was identified in 45 episodes (20.1%): chlamydia pneumoniae (14 episodes), haemophilus influenzae type b (1), influenza virus type a (14), respiratory syncytial virus (rsv;2), parainfluenza virus type 3 (7), c. pneumoniae and h. in ... | 1996 | 8953065 |
| developmental and environmental factors that enhance binding of bordetella pertussis to human epithelial cells in relation to sudden infant death syndrome (sids). | asymptomatic infection due to bordetella pertussis has been suggested to be one cause of sudden infant death syndrome (sids). we examined developmental and environmental factors previously found to affect binding of another toxigenic species, staphylococcus aureus, to human epithelial cells: expression of the lewis(a) antigen; infection with respiratory syncytial virus (rsv); exposure to cigarette smoke; and the inhibitory effect of breast milk on bacterial binding. binding of two strains of b. ... | 1996 | 8954353 |
| morphological studies of the respiratory syncytial virus induced bronchiolitis in experimentally infected calves. | rsv-infections of the lower respiratory tract in infancy and early childhood are the most frequent causes of a hyperreactive bronchial system and obstructive lung disease. studies concerning the morphological alterations of the bronchial mucosa during an rsv-infection are dependent on an experimental animal model. in this study the alterations of the lower respiratory tract from five infected colostrum-fed calves during the initial stage of the infection are described. brsv strain 375 was applie ... | 1996 | 8958555 |
| safety and immunogenicity of a respiratory syncytial virus subunit vaccine (pfp-2) in ambulatory adults over age 60. | the safety and immunogenicity of purified fusion protein (pfp-2) respiratory syncytial virus (rsv) vaccine was evaluated in a randomized placebo-controlled, double-blind study of 64 healthy adults over age 60. vaccination was well tolerated with no significant acute side-effects. twenty-nine of 33 vaccinees (87%) showed a greater than or equal to fourfold rise in serum igg to the f protein of rsv at 8 weeks post vaccination. twenty of 33 vaccine recipients (61%) had a greater than or equal to fo ... | 1996 | 8961507 |
| [characteristics of anti-respiratory syncytial humoral immunity during persistence of respiratory syncytial virus antigens in adult patients with chronic obstructive bronchitis]. | specific humoral immune response has been studied in 8 patients with chronic obstructive bronchitis, in whom the persistence of rsv was detected, and in a group of patients without rsv persistence. humoral immunity in patients with rsv persistence vs. those with acute and chronic bronchitis without viral persistence was characterized by the following features: a) earlier and more active production of igg and, even more so, igm and iga; b) long persistence of high titers of anti-rsv igg, igm, and ... | 1996 | 8967073 |
| ribavirin for rsv lower respiratory tract infection. | 1996 | 8969696 | |
| prevention of respiratory syncytial virus infection in high risk infants: consensus opinion on the role of immunoprophylaxis with respiratory syncytial virus hyperimmune globulin. | 1996 | 8970212 | |
| frequency of respiratory syncytial virus in hospitalized infants with lower acute respiratory tract infection in colombia. | in colombia, health workers obtained a nasopharyngeal wash from 103 infants aged less than 12 months hospitalized for acute lower respiratory infection (alri) at the general hospital of medellin during april 1994 to april 1995 so researchers could determine the frequency of alri caused by respiratory syncytial virus (rsv) in hospitalized children. immunofluorescence detected rsv infection in 43 (41.7%) patients. the presence of the following signs and symptoms allowed a clinical diagnosis of a ... | 1996 | 8970224 |
| transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the rela transcription factor as a mechanism producing airway mucosal inflammation. | the most common cause of epidemic pediatric respiratory disease, respiratory syncytial virus (rsv), stimulates interleukin-8 (il-8) synthesis upon infecting airway epithelium, an event necessary for the development of mucosal inflammation. we investigated the mechanism for enhanced il-8 production in human a549 type ii pulmonary epithelial cells. infection with sucrose-purified rsv (prsv) produced a time-dependent increase in the transcriptional initiation rate of the il-8 gene. transient transf ... | 1996 | 8971006 |
| inhibition of viral replication reverses respiratory syncytial virus-induced nf-kappab activation and interleukin-8 gene expression in a549 cells. | previous studies have demonstrated that respiratory syncytial virus (rsv) infection of airway epithelial cells results in the expression of a number of cytokines, such as interleukin-8 (il-8), that are transcriptionally regulated by nuclear factor kappab (nf-kappab). in the studies reported here, we demonstrate that treatment of rsv-infected a549 cells with 100 microg of ribavirin (a viral replication inhibitor) per ml results in reversal of rsv-induced nf-kappab activation, il-8 mrna expression ... | 1996 | 8971048 |
| influenza surveillance in england and wales: october 1995 to june 1996. | this report summarises information collected for the surveillance of influenza virus infection in england and wales from october 1995 to june 1996 (weeks 40/95 to 25/96). total respiratory disease' activity, as reported by the birmingham research unit of the royal college of general practitioners, rose to peaks in weeks 48/95, 51/95, and 01/96. the first peak coincided with a peak in "influenza and flu-like illness'. the subsequent peaks were accounted for by an increase in reports of acute bron ... | 1996 | 8972979 |
| respiratory syncytial virus-induced cytokine production by neonatal macrophages. | the induction of immunoregulatory cytokines il-1 beta, il-6, il-12, tumour necrosis factor-alpha (tnf-alpha) and interferon-gamma (ifn-gamma) was studied with neonatal (cord blood) monocyte-derived macrophages (mdm) after in vitro infection with respiratory syncytial virus (rsv). the expression of mrnas for these cytokines in rsv-infected mdm was examined by reverse transcriptase-polymerase chain reaction (rt-pcr). the activities of these cytokines were assayed by elisa. significant increase of ... | 1996 | 8973610 |
| update: respiratory syncytial virus activity--united states, 1996-97 season. | respiratory syncytial virus (rsv), a common cause of winter outbreaks of acute respiratory disease, results in an estimated 90,000 hospitalizations and 4500 deaths each year from lower respiratory tract disease in both infants and young children in the united states. outbreaks occur annually throughout the country. rsv activity in the united states is monitored by the national respiratory and enteric virus surveillance system (nrevss), a voluntary, laboratory-based system. this report summarizes ... | 1996 | 8975116 |
| icam-1 expression and low-molecular-weight g-protein activation of human bronchial epithelial cells (a549) infected with rsv. | the airway epithelial cell of the lower respiratory tract is the primary host target cell for respiratory syncytial virus (rsv) infection. to estimate whether infected epithelial cells contribute to the inflammatory host response observed during the acute infection phase we analyzed the cell surface expression of intercellular adhesion molecule-1 (icam-1, cd54) on human bronchial epithelial cells (a549) following rsv infection and cytokine priming. the epithelial cells constitutively expressed i ... | 1996 | 8975880 |
| [epidemiology of acute bronchopulmonary infections in children]. | in infants and young children acute lower respiratory infection is the most common cause of morbidity and death especially in developing countries. factors that contribute to the increased susceptibility to respiratory pathogens include young age, season, sex, indoor pollution, large family size, malnutrition, low immunocompetence, socioeconomic disadvantage. the epidemiology of acute respiratory infections in childhood seems similar worldwide. in all countries, respiratory syncytial virus, para ... | 1996 | 8978159 |
| from the centers for disease control and prevention. update: respiratory syncytial virus activity--united states, 1996-97 season. | 1997 | 8980197 | |
| increase in cd23+ b cells in infants with bronchiolitis is accompanied by appearance of ige and igg4 antibodies specific for respiratory syncytial virus. | infection with respiratory syncytial virus (rsv) may induce asthma-like symptoms and rsv-specific ige in infected infants as a result of th2-like response to rsv. the effect of rsv infection on the expression of b cell antigens cd21 and cd23, putative participants in th2 responses, was investigated. samples from bronchiolitic infants (n = 19) were tested by three-color immunofluorescence flow cytometry during the acute phase of infection and 4-6 weeks later. in 6 of 10 rsv-positive infants, the ... | 1997 | 8985193 |
| virus-specific memory and effector t lymphocytes exhibit different cytokine responses to antigens during experimental murine respiratory syncytial virus infection. | mice sensitized to the g (attachment) or f (fusion) glycoproteins of respiratory syncytial virus (rsv) expressed different patterns of cytokine production and lung pathology when challenged by intranasal infection with rsv. five days after challenge, mice sensitized to g glycoprotein produced high levels of interleukin-4 (il-4) and il-5 in the lungs and spleens and developed extensive pulmonary eosinophilia, while mice sensitized to f glycoprotein produced il-2 and developed a mononuclear cell i ... | 1997 | 8985399 |
| identification of potential ctl epitopes of bovine rsv using allele-specific peptide motifs from bovine mhc class i molecules. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in young infants and housed calves. depletion of cd8+ lymphocytes from calves inhibited their ability to clear the virus from the nasopharynx and lungs. to study these cells further, a cytotoxic t lymphocyte (ctl) assay was established. ctl could be demonstrated in the peripheral blood of gnotobiotic calves 7-10 days post infection (p.i.) with rsv and in lungs 10 days p.i. this response was both mhc-restricte ... | 1996 | 8988867 |
| reduction of respiratory syncytial virus hospitalization among premature infants and infants with bronchopulmonary dysplasia using respiratory syncytial virus immune globulin prophylaxis. the prevent study group. | to determine the safety and efficacy of monthly prophylaxis with respiratory syncytial virus immune globulin, intravenous (rsv-igiv) for reduction of the incidence of rsv-associated hospitalization. | 1997 | 8989345 |
| outbreaks of influenza and a rise in respiratory syncytial virus: winter has arrived. | 1996 | 8990578 | |
| stabilization of respiratory syncytial virus (rsv) against thermal inactivation and freeze-thaw cycles for development and control of rsv vaccines and immune globulin. | a high-titered and stable respiratory syncytial virus (rsv) is essential for the development of rsv vaccines and quality control of vaccines and rsv immune globulin. we increased the virus titer of rsv seed stock, and virus preparations made from this seed stock, 100 times by removing defective interfering particles using limiting dilution procedure. rsv preparations made from the new seed stock had infectivity titers ranging from 10(7.6) to 10(8.2) tcid50 per ml for five lots made over a period ... | 1996 | 8994316 |
| mechanism of rsv-induced il-8 gene expression in a549 cells before viral replication. | previous studies demonstrated that respiratory syncytial virus (rsv) infection of a549 cells induced interleukin (il)-8 gene expression and protein release from the cells as early as 2 h after treatment [m. a. fiedler, k. wernke-dollries, and j. m. stark. am. j. physiol. 269 (lung cell. mol. physiol. 13): l865-l872, 1995; j. g. mastronarde, m. m. monick, and g. w. hunninghake. am. j. respir. cell mol. biol. 13: 237-244, 1995]. furthermore, the effects of rsv at the 2-h time point were not depend ... | 1996 | 8997267 |
| respiratory syncytial virus immune globulin intravenous. | to review the clinical data detailing the use of respiratory syncytial virus immune globulin intravenous (rsv-igiv) in infants and children. data sources; a medline search (1990-1996) was used to identify all publications that dealt with rsv-igiv clinical trials, pharmacology, and pharmacokinetics in infants and children. bibliographies of articles were also used. | 1997 | 8997472 |
| rhinovirus infection associated with serious lower respiratory illness in patients with bronchopulmonary dysplasia. | to determine the characteristics of rhinovirus infection in patients with bronchopulmonary dysplasia. | 1997 | 9002100 |
| vitamin a therapy for respiratory syncytial virus infection. | 1997 | 9002114 | |
| white blood cell count, c-reactive protein and erythrocyte sedimentation rate in respiratory syncytial virus infection of the lower respiratory tract. | laboratory findings such as white blood cell (wbc) count, c-reactive protein (crp) concentration and erythrocyte sedimentation rate (esr) level in patients with bronchiolitis, bronchopneumonia and lobar pneumonia caused by respiratory syncytial virus (rsv) were studied. the diagnosis of having rsv infection of the lower respiratory tract was made on the presence of rsv antigen in nasopharyngeal specimens by means of enzyme immunoassay, on chest x-ray appearances and clinical manifestations. the ... | 1996 | 9002292 |
| detection of antibodies to respiratory syncytial virus attachment and nucleocapsid proteins with recombinant baculovirus-expressed antigens. | the ability to measure antibodies against individual respiratory syncytial virus (rsv) proteins is important in the analysis of immune responses to rsv. we expressed the nucleocapsid (n) protein and the group a and b rsv attachment (g) proteins from recombinant baculoviruses. the three recombinant rsv proteins were used individually in an enzyme-linked immunosorbent assay (elisa; bac-elisa for results from assays of all three proteins). the bac-elisa results were compared to the results obtained ... | 1997 | 9003595 |
| prevalence of antibodies against hep-2 cell antigen in infants and children hospitalized with respiratory syncytial virus infection. | infants with respiratory syncytial virus (rsv) infection were shown to have antibodies against hep-2 cell antigen present in rsv-antigen preparation used for immunoblot analysis. the prevalence of anti hep-2 cell antibodies was examined in infants hospitalized for rsv infection (n = 49, median age 121 days) compared to rotavirus infected children (n = 30, median age 114 days) and to healthy controls (n = 20, median age 150 days). the immunoblot analysis with rsv-infected and non-infected hep-2 c ... | 1996 | 9007586 |
| subgrouping of bovine respiratory syncytial virus strains detected in lung tissue. | bovine respiratory syncytial virus is an important respiratory pathogen in cattle. recently, subgroups of brsv have been identified, based on antigenic differences. however, little is known about subgroups of brsv that circulate in the cattle population. therefore, we determined the reactivity of monoclonal antibodies (mabs), directed against the g, f, or p protein of brsv, with lung tissue from 47 calves, that suffered from severe respiratory disease. fourteen animals (30%) proved to be infecte ... | 1996 | 9008336 |
| functional analysis of antibody responses of feedlot cattle to bovine respiratory syncytial virus following vaccination with mixed vaccines. | the antibody response of cattle to bovine respiratory syncytial virus (brsv) immunization was investigated using 4 different commercially available mixed vaccines. forty, 5-6 month old, beef calves, randomly assigned to groups of 10, were vaccinated on day 0 and 21 with 1 of 3 inactivated vaccines, (3 groups), or a modified live virus (mlv) vaccine. brsv-specific antibody responses were measured prior to vaccination and on day 35 by using an enzyme linked immunosorbent assay (elisa), virus neutr ... | 1997 | 9008797 |
| [infectious complications of lung and heart-lung transplantation]. | thanks to a simplification of surgical techniques, single or double lung transplants have expanded significantly in latter years. infection remains an important cause for morbidity and mortality, more so in early rather than late stages. bacterial infections cause approximately fifty per cent of all infections. they can be prevented in part by prophylaxis. infections to cmv have become less frequent thanks to adequate prophylaxis with ganciclovir. herpetic infections are prevented by acyclovir o ... | 1996 | 9011910 |
| [respiratory syncytial virus: an important cause of influenza-like disese in the elderly]. | 1996 | 9012077 | |
| maturation of respiratory syncytial virus within hep-2 cell cytoplasm. | electron microscopy of hep-2 cells infected with respiratory syncytial virus (rsv) strain long revealed the maturation of rsv on an ultrastructural level. the results showed that the virus maturated by two different pathways. in one of them, the virus assembled and matured before reaching the plasma membrane on the internal vesicle membrane within cytoplasm. the mature virus was delivered to the plasma membrane and to the extracellular space most likely by the transport vesicles and exocytosis. ... | 1996 | 9014011 |
| treatment of respiratory failure due to respiratory syncytial virus pneumonia with natural surfactant. | we describe two infants suffering from severe pneumonia caused by respiratory syncytial virus (rsv) infection and needing mechanical ventilation with both high ventilator settings and a high fraction of inspired oxygen. the severity of the respiratory failure and the possibility of decreased and/or altered surfactant production led us to treat these infants with intratracheal instillation of natural surfactant. this resulted in an improvement of lung compliance and a decrease in the amount of ox ... | 1996 | 9016476 |
| antigenic and molecular analyses of the variability of bovine respiratory syncytial virus g glycoprotein. | antigenic variation among eight bovine respiratory syncytial virus (brsv) isolates was determined using monoclonal antibodies (mabs) specific for the attachment (g) protein. two major (and one intermediate) subgroups were identified, as well as one strain that did not fit any pattern. the subgroups could also be differentiated on the basis of the mr of the f protein cleavage product, f2. the nucleotide sequence of the g gene of seven of the brsv strains was determined and compared with published ... | 1997 | 9018058 |
| serological studies of bovine respiratory syncytial virus in feedlot cattle in south africa. | serum antibody titres to bovine respiratory syncytial virus were determined in cattle (n = 282). samples were obtained at various feedlots and were collected in the 1989 and 1990 winter seasons, during the course of investigations into outbreaks of respiratory tract infections in feedlot cattle. results of the survey revealed an antibody prevalence of 43% to bovine respiratory syncytial virus. titres ranged from 1:10 to 1:1280 as determined by the indirect fluorescent antibody test. the results ... | 1990 | 9022846 |
| a multivalent minigene vaccine, containing b-cell, cytotoxic t-lymphocyte, and th epitopes from several microbes, induces appropriate responses in vivo and confers protection against more than one pathogen. | the development of safe and effective vaccines remains a major goal in the prevention, and perhaps treatment, of infectious diseases. ideally, a single vaccine would confer protection against several pathogens and would induce both cellular and humoral arms of the immune response. we originally demonstrated that two virus-specific cytotoxic t-lymphocyte (ctl) epitopes, from the same virus but presented by different major histocompatibility complex alleles, when linked in tandem as minigenes in a ... | 1997 | 9032365 |
| persistence of respiratory syncytial virus (rsv) infection and development of rsv-specific igg1 response in a guinea-pig model of acute bronchiolitis. | acute respiratory syncytial virus (rsv) bronchiolitis in children can result in sequelae of recurrent wheezing and asthma and production of rsv-specific immunoglobulin e (ige), but the pathogenesis of these sequeleae is poorly understood. guinea-pigs experimentally inoculated with human rsv show histological evidence of acute bronchiolitis and chronic persistence of viral antigens and genome in the lungs; whether this persistence is due to infectious replicating virus, and whether infected anima ... | 1997 | 9032486 |
| identification of mutations contributing to the reduced virulence of a modified strain of respiratory syncytial virus. | the nucleotide sequences of the genome of the rss-2 wild type strain of respiratory syncytial (rs) virus, which is known to induce upper respiratory tract infection in adults, and that of the attenuated ts1c candidate vaccine derived from it by three cycles of mutagenesis and selection of temperature-sensitive (ts) mutants, have been determined. comparison of the sequences has located the genetic changes which contribute to the reduced pathogenicity in adults of the candidate vaccine. thirty-sev ... | 1996 | 9032893 |
| acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (l) gene. | a cold-passaged (cp) temperature-sensitive (ts) mutant of human respiratory syncytial virus designated rsv cpts-248 was previously derived by random chemical mutagenesis of the non-ts mutant cp-rsv that possesses one or more host range mutations. we previously demonstrated in rodents and seronegative chimpanzees that the cpts-248 virus is more attenuated than cp-rsv and is more stable genetically than previously isolated rsv ts mutants. in the present study, we determined that the acquisition of ... | 1996 | 9035372 |
| [etiological and clinical evaluation of low acute respiratory infections in children]. | viral laboratory diagnosis was correlated with clinical and epidemiological data from 80 hospitalized children with acute lower respiratory infection (alri). they all were less than 5 years-old and were studied from may to september 1993. fifteen percent of them were malnourished and 75% had some unsatisfied basic necessity. nasopharingeal aspirates were obtained the first day of hospitalization, and diagnosis for respiratory viruses was performed by the immunofluorescence test with monoclonal a ... | 1996 | 9035475 |
| guidelines for prevention of nosocomial pneumonia. centers for disease control and prevention. | this document updates and replaces cdc's previously published "guideline for prevention of nosocomial pneumonia" (infect control 1982;3:327-33, respir care 1983;28:221-32, and am j infect control 1983;11:230-44). this revised guideline is designed to reduce the incidence of nosocomial pneumonia and is intended for use by personnel who are responsible for surveillance and control of infections in acute-care hospitals; the information may not be applicable in long-term-care facilities because of t ... | 1997 | 9036304 |
| inhibition of respiratory syncytial virus replication by antisense oligodeoxyribonucleotides. | oligodeoxyribonucleotides targeted against respiratory syncytial virus (rsv) genomic rna inhibited rsv replication in cell culture by an apparent antisense mechanism. hep-2 cells were infected with rsv strain a2 and incubated in the presence of oligonucleotides. virus replication was measured by enzyme-linked immunosorbent assay (elisa), virus yield assay, or production of specific rsv mrnas. using elisa, 50% effective concentration (ec50) values were about 0.5-1 microm for an antisense oligonuc ... | 1997 | 9037376 |
| respiratory syncytial virus (rsv) immune globulin intravenous therapy for rsv lower respiratory tract infection in infants and young children at high risk for severe rsv infections: respiratory syncytial virus immune globulin study group. | to evaluate the efficacy of high-titer intravenous respiratory syncytial virus immune globulin (rsvig) in the treatment of children at high risk for severe rsv infection who were hospitalized with proven rsv. | 1997 | 9041304 |
| reducing the morbidity of lower respiratory tract infections caused by respiratory syncytial virus: still no answer. | 1997 | 9041306 | |
| respiratory syncytial virus induces selective production of the chemokine rantes by upper airway epithelial cells. | the presence of histamine and eosinophil cationic protein in nasopharyngeal secretions of infants with respiratory syncytial virus (rsv)-induced bronchiolitis implies the activation of basophil and eosinophil leukocytes, but the specific mechanism of their recruitment has not been elucidated. chemokines are potent and selective leukocyte chemotactic molecules that are also expressed by airway epithelial cells. therefore, the pattern of chemokines produced in response to rsv infection was investi ... | 1997 | 9041319 |
| a common ailment. | 1997 | 9043336 | |
| extracorporeal life support for the treatment of viral pneumonia: collective experience from the elso registry. extracorporeal life support organization. | viral pneumonia is the most common indication for pediatric extracorporeal life support (ecls). despite this fact, no previous studies have directly stratified patient outcome according to viral etiology. | 1997 | 9044128 |
| targeting rna decay with 2',5' oligoadenylate-antisense in respiratory syncytial virus-infected cells. | treatment of human cells with 2',5' oligoadenylate covalently linked to antisense (2-5a-antisense) results in the selective cleavage of targeted rna species by 2-5a-dependent rnase l. here we show that 2-5a-antisense containing stabilizing modifications at both termini are effective in suppressing the replication of respiratory syncytial virus (rsv) in human tracheal epithelial cells. the affinity of 2-5a-antisense for different regions in the rsv m2 and l mrnas was predicted from a computer-gen ... | 1997 | 9050883 |
| expression in escherichia coli and purification of soluble forms of the f protein of bovine respiratory syncytial virus. | six fragments of the f gene from bovine respiratory syncytial virus (brsv) were engineered into the pmal-c2 escherichia coli expression vector and expressed as c-terminal maltose-binding protein (mbp) fusion products. the resulting polypeptides were partially soluble and single-step purified by affinity chromatography. these fusion proteins were recognized in western blots by several mabs directed against human respiratory syncytial virus f protein. in addition, rabbit polyclonal antisera raised ... | 1997 | 9056494 |
| respiratory syncytial virus infection among hospitalized young children with acute lower respiratory illnesses in al ain, uae. | to establish the frequency and clinical pattern of respiratory syncytial virus (rsv) infection in the region, children under 3 years of age admitted for acute lower respiratory illness during two winter seasons of the years 1993-94 and 1994-95 were studied prospectively. seventy two cases were diagnosed to have rsv infection among the 252 studied, representing 28.57% of these patients. the overall infection rate was 32.1% and 36.5% respectively for the two studied winter seasons. among these chi ... | 1996 | 9057448 |
| improved oxygenation after discontinuing neuromuscular blockade. | to evaluate the effects of prolonged neuromuscular blockade (nmb) on oxygenation and duration of mechanical ventilation in children with respiratory failure. | 1997 | 9069009 |
| rapid detection of respiratory syncytial virus by indirect immunofluorescence in infants hospitalized with acute respiratory disease. | 1996 | 9070408 | |
| respiratory syncytial virus infection in the elderly. | respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract infections in infants and children throughout the world. respiratory syncytial virus infections in the elderly represent reinfections in the hosts who have had many prior episodes. thus, rsv infections are usually not considered serious in adults, since reinfections are generally known to result in mild disease. nevertheless, in adults, as in children, the infection has been reported to cause altered airway res ... | 1996 | 9072495 |
| persistence of airway hyperresponsiveness and viral antigen following respiratory syncytial virus bronchiolitis in young guinea-pigs. | respiratory syncytial virus (rsv) bronchiolitis in infancy is known to be followed by chronic respiratory symptoms and airway hyperresponsiveness in a subgroup of patients. to further investigate the pathogenesis of rsv-induced chronic airway pathology, we infected young guinea-pigs at 4 weeks of age with rsv applied as an aerosol (n=30), and control guinea-pigs with virus-free culture medium (n=24). infection was confirmed by positive antibody titre to rsv after 6 weeks, and by typical patholog ... | 1997 | 9072998 |
| respiratory syncytial virus triggers synthesis of il-6 in balb/c mouse alveolar macrophages in the absence of virus replication. | cytokines produced by alveolar macrophages are likely involved in the regulation of the immune response arising from respiratory syncytial virus (rsv) infection. both infectious and uv-inactivated rsv were effective in inducing balb/c mouse alveolar macrophages to synthesize increased levels of il-6 mrna and secreted il-6 protein. no increase in il-1beta (either mrna or secreted protein) was observed. the augmented production of il-6 was activated by purified virus and was reduced by pretreating ... | 1997 | 9073384 |
| assault against respiratory syncytial virus: beginning of an era. | 1997 | 9074568 | |
| follow-up of children with respiratory syncytial virus bronchiolitis in 1986 and 1987: potential effect of ribavirin on long term pulmonary function. the bronchiolitis study group. | hospitalization for respiratory syncytial virus (rsv) bronchiolitis is considered a risk factor for reactive airway disease and/or abnormal pulmonary function tests (pfts) later in childhood. this study attempted to assess this observation with newer pfts and to determine whether ribavirin therapy of initial rsv infection may affect the long term outcome. | 1997 | 9076814 |
| [radiological aspects of acute respiratory disorders of childhood caused by influenzae, parainfluenzae and respiratory syncytial viruses]. | 1970 | 9082353 | |
| severity of respiratory syncytial virus infection is related to virus strain. | the relationship between respiratory syncytial virus (rsv) strain and disease severity was assessed in 265 hospitalized infants over a 3-year period (1988-1991). a severity index of clinical and physiologic parameters was used to grade illness severity. multivariate analysis of 134 infants infected with group a rsv strains and 131 infants infected with group b strains indicated that prematurity, underlying medical conditions, group a rsv infection, and age < or =3 months were independently assoc ... | 1997 | 9086135 |
| immunoprophylaxis of group b respiratory syncytial virus infection in cotton rats. | two antigenic groups of respiratory syncytial virus (rsv) have been identified: a (rsv/a) and b (rsv/b). topical administration of human igg screened for high titers of antibody to rsv/a (rsvig) is protective against rsv/a infection in the cotton rat model. the study attempted to determine if topical rsvig would also be protective against rsv/b. cotton rats were pretreated intranasally with rsvig or with monospecific antiserum obtained from animals previously infected with rsv/a or rsv/b (day 0) ... | 1997 | 9086152 |
| the guinea pig as a model for the study of maternal immunization against respiratory syncytial virus infections in infancy. | maternal immunization might protect infants from severe disease due to respiratory syncytial virus (rsv). guinea pigs are susceptible to infections with rsv and transfer antibodies to their offspring prenatally. pregnant guinea pigs were immunized by infection with rsv and their offspring were challenged intranasally with rsv. pulmonary viral replication was compared among the pups born to immunized mothers (group a) and the pups from nonimmune mothers (group b) in two studies. mean (+/-sd) log1 ... | 1997 | 9086153 |
| respigam: an rsv immune globulin. | respiratory syncytial virus immune globulin has been shown to prevent or attenuate rsv lower respiratory tract infection in infants under 24 months of age with bpd or who were born prematurely (< or = 35 weeks gestation). the use of rsv-ig is expensive. the estimated average cost of prophylaxis for a season is $4,000 to $5,000 per infant. because of the cost of rsv-ig, and because viral transmission is possible when any blood product is administered, careful consideration of which children shoul ... | 1996 | 9087091 |
| standardization of enzyme-linked immunosorbent assays (elisas) for quantitative estimation of antibodies specific for infectious bovine rhinotracheitis virus, respiratory syncytial virus, parainfluenza-3 virus, and bovine viral diarrhea virus. | commercial enzyme-linked immunosorbent assays (elisas) for detection of serum antibodies to bovine viral diarrhea virus (bvdv), parainfluenza-3 virus (pi3v), respiratory syncytial virus (rsv), and infectious bovine rhinotracheitis virus (ibrv) were standardized to give a quantitative result when testing was performed at a single optimum dilution. for each test, serum samples were titrated and their end point titers calculated by an algebraic method directly from a plot of each titration series a ... | 1997 | 9087921 |
| advances in antiviral therapy. | for many years, acyclovir has been used to treat herpes simplex and varicella zoster infections in adults and children, although new drugs with improved bioavailability and dosage regimens (ie, famciclovir, valaciclovir) are replacing it for the outpatient management of these conditions in adults. acyclovir remains the treatment of choice for severe herpes infections in both immunocompetent and immunosuppressed patients. data on the newer antiherpes drugs in children are not available. treatment ... | 1997 | 9088751 |
| respiratory syncytial virus bronchiolitis. | respiratory syncytial virus (rsv) bronchiolitis is associated with the clinical signs and symptoms of small airway obstruction. a major public health problem throughout the world, this condition is responsible for significant morbidity and mortality. management is primarily preventive, through strict hand washing, avoidance of exposure during the respiratory illness season and intravenously administered prophylactic anti-rsv immune globulin, especially in selected small infants with underlying c ... | 1997 | 9092277 |
| respiratory syncytial virus immune globulin intravenous: indications for use. american academy of pediatrics committee on infectious diseases, committee on fetus and newborn. | respiratory syncytial virus immune globulin intravenous (rsv-igiv) has been approved by the food and drug administration for use in the prevention of severe rsv infections in infants and children younger than 24 months with bronchopulmonary dysplasia or a history of premature birth (< or = 35 weeks of gestation). rsv-igiv administered monthly during the rsv season resulted in a 41% to 65% reduction in hospitalization rates in two clinical trials; however, rsv-igiv is costly, and intravenous admi ... | 1997 | 9093323 |
| analysis of linear epitopes recognised by the primary human antibody response to a variable region of the attachment (g) protein of respiratory syncytial virus. | the sites of linear epitopes in a variable region of the attachment (g) glycoprotein of respiratory syncytial virus (rsv) that are recognised by the human antibody response were examined. two sets of overlapping 12mer peptides each representing the carboxy-terminal 84 or 85 amino acids of the g protein of two group a isolates of human rsv were synthesised. these peptides were analysed using enzyme-linked immunosorbant assays (elisa) for their reactions with sera obtained from infants with primar ... | 1997 | 9093944 |
| antigenic structure of the central conserved region of protein g of bovine respiratory syncytial virus. | epitopes were resolved at the amino acid level for nine monoclonal antibodies (mabs) directed against the central conserved region of protein g of bovine respiratory syncytial virus (brsv-g). peptide binding studies showed which amino acids in the epitope contributed to antibody binding. the details of the epitopes were compared with the high-resolution structure of a synthetic peptide corresponding to the central conserved region of brsv-g, and this indicated which face of the central conserved ... | 1997 | 9094683 |
| acute respiratory distress syndrome caused by respiratory syncytial virus. | acute respiratory distress syndrome (ards) complicating severe respiratory syncytial virus (rsv) infection has been described in only a few infants. in contrast to the low mortality rates usually associated with rsv infections (< 5%), mortality rates in the range of 40-70% have been reported in pediatric patients with ards. however, studies on patients with ards are usually lumped with respect to causation, and the disease course of rsv-induced ards has not been previously studied. we examined t ... | 1997 | 9094725 |
| risk factors for bronchiolitis: presentation of an on-going prospective clinical study. | 1997 | 9098778 | |
| effect of human milk prostaglandins and lactoferrin on respiratory syncytial virus and rotavirus. | the effect of lactoferrin and prostaglandins e and f2 alpha on the growth of rotavirus and respiratory syncytial virus in cell culture was investigated. lactoferrin inhibited the growth of respiratory syncytial virus at a concentration tenfold lower than that normally present in human milk. the prostaglandins had no effect on either virus growth, even at a concentration of 100-fold more than that found in human milk. lactoferrin may have some antiviral properties in human milk in addition to its ... | 1997 | 9099323 |
| other viral agents of perinatal importance. varicella, parvovirus, respiratory syncytial virus, and enterovirus. | this article summarizes new information on the natural history of maternal varicella and maternal parvovirus b19. controversies in the therapy of respiratory syncytial virus are presented. prophylactic and therapeutic immunotherapy of neonatal enteroviral disease are reviewed. | 1997 | 9099510 |
| twenty years of outpatient respiratory syncytial virus infection: a framework for vaccine efficacy trials. | respiratory syncytial virus (rsv) is the most important viral respiratory pathogen of infancy and childhood. much has been written about inpatients with severe disease. inpatients, however, represent only a minority of rsv-infected children. we studied the characteristics of symptomatic outpatient rsv infection in healthy children to gain a better understanding of rsv disease and to provide a background for the testing of intervention strategies in children without high-risk conditions. | 1997 | 9099764 |
| does ribavirin impact on the hospital course of children with respiratory syncytial virus (rsv) infection? an analysis using the pediatric investigators collaborative network on infections in canada (picnic) rsv database. | to determine the relationship between receipt of aerosolized ribavirin and the hospital course of high-risk infants and children with respiratory syncytial virus (rsv) lower respiratory infection (lri). | 1997 | 9099772 |
| picnic (pediatric investigators collaborative network on infections in canada) study of the role of age and respiratory syncytial virus neutralizing antibody on respiratory syncytial virus illness in patients with underlying heart or lung disease. | to determine the effects of age and respiratory syncytial virus (rsv) antibody status on frequency and severity of rsv infections in children with underlying heart or lung disease. | 1997 | 9099774 |
| sequence conservation in the attachment glycoprotein and antigenic diversity among bovine respiratory syncytial virus isolates. | partial nucleotide sequences were determined from the coding regions of the attachment glycoprotein (g) mrnas of eight isolates of bovine respiratory syncytial virus (brsv). the antigenic characteristics of 18 field and reference isolates were analyzed using the reactivity patterns of monoclonal antibodies (mabs) directed against the human respiratory syncytial virus (hrsv) and brsv g. fusion protein (f), nucleoprotein (n), and phosphoprotein (p), by radioimmunoprecipitation and immunofluorescen ... | 1997 | 9100323 |
| infant respiratory function after rsv-proven bronchiolitis. | the mechanisms underlying the increased risk of wheezing in early childhood following acute bronchiolitis in infancy remain unclear. previous studies have reported significant abnormalities in infant respiratory function after clinical recovery from bronchiolitis, but are difficult to interpret because of the frequent omission of a concurrent comparison group. respiratory function was compared within pairs of previously healthy full-term caucasian infants admitted with a first episode of acute b ... | 1997 | 9105078 |
| experimental infections with some pneumotropic viruses in the mouse. note i. virological and pathomorphological aspects of the associated infections with influenza virus, adenovirus and respiratory syncytial virus. | in white mice, the associated infections with the influenza virus type a(h3n2), the adenovirus type 3 and the long strain of the respiratory syncytial virus, detected by the appearance of homologous serum antibodies and by positive i.f. reactions in pulmonary tissue, induce histological, histochemical and histo-enzymatic lesions, the severity of which exceeds that of the lesions in animals infected with only one of the mentioned viruses. in comparison with the morphological picture in animals in ... | 1995 | 9106398 |
| potent inhibition of respiratory syncytial virus by polyoxometalates of several structural classes. | a series of polyoxometalates (pom) were synthesized and evaluated for anti-respiratory syncytial virus (rsv) activity. pom containing zirconium, tungsten, silicon, platinum, niobium or germanium of a variety of structural types have been evaluated. sixteen of the compounds had very striking anti-rsv activity against a clinical isolate, utah 89, with median effective concentration (ec50) values < or = 3 microm and selective indices > 80 as determined by viral cytopathic inhibition effect, neutral ... | 1997 | 9107383 |
| rsv pneumonia, a community-acquired infection in adults. | 1997 | 9111535 | |
| [a potential rapid immunoperoxidase method of diagnosis in infections due to the respiratory syncytial virus, parainfluenza viruses and adenoviruses]. | 1996 | 9116403 | |
| rsv infection of human airway epithelial cells causes production of the beta-chemokine rantes. | infection of airway epithelial cells with respiratory syncytial virus (rsv) results in the production of a restricted number of cytokines, which may modulate the inflammatory response to infection. to get a better understanding of epithelial cell-mediated inflammatory processes in rsv disease, the aim of the present study was to identify the production of mononuclear cell/eosinophil/mast cell inflammatory chemokines [monocyte chemotactic protein (mcp)-1, mcp-3, macrophage inflammatory protein-1b ... | 1997 | 9124609 |
| expression of mxa protein in blood lymphocytes discriminates between viral and bacterial infections in febrile children. | interferons (ifns), which are induced by viruses, form an essential part of host's defense systems against viral infections. the antiviral actions of ifns are mediated by several ifn-inducible gene products, one of which is mx protein. to evaluate whether mxa protein expression in lymphocytes could function as an indicator of endogenous ifn production in children with acute febrile illness, we analyzed mxa protein levels in peripheral blood lymphocytes by flow cytometry in the acute phase of the ... | 1997 | 9128286 |
| enhanced cytokine production by milk macrophages following infection with respiratory syncytial virus. | paired samples of milk and serum collected 3 days postpartum from 20 women were tested for the presence and level of interleukin (il)-1, il-6, il-12, tumor necrosis factor alpha (tnf-alpha), and interferon-gamma (ifn-gamma) by enzyme immunoassay. the expression of these cytokine mrnas in milk macrophages from eight donors were semiquantitatively analyzed by reverse transcriptase-polymerase chain reaction. the effects of respiratory syncytial virus (rsv) infection on cytokine production were dete ... | 1997 | 9129213 |
| respiratory syncytial virus specific serum antibodies in infants under six months of age: limited serological response upon infection. | the decline of maternal respiratory syncytial virus (rsv) specific serum antibodies was studied in 45 children during the first 6 months of life, using a virus neutralization assay and competition elisas measuring fusion protein and glycoprotein specific antibodies. in all children rsv neutralizing antibodies were demonstrated at birth, with titers ranging from 33 to 1382. the calculated mean half life of these antibodies was 26 days. furthermore, in a group of 38 children with suspected rsv inf ... | 1997 | 9131465 |
| serologic survey for infectious pathogens in free-ranging american bison. | from november 1991 through march 1992, we evaluated 101 free-ranging american bison (bison bison) from yellowstone national park, wyoming (usa) for exposure to infectious organisms that commonly infect cattle. no titers were detected for bluetongue virus, bovine leukemia virus, or campylobacter fetus in these 101 bison. detectable antibodies occurred against anaplasma marginale (eight of 76, 11%), bovine respiratory syncytial virus (31 of 101, 31%), bovine viral diarrhea (31 of 101, 31%), bovine ... | 1997 | 9131564 |
| immunological responses to respiratory syncytial virus infection in infancy. | to determine whether there is evidence of immunological responses in infants with respiratory syncytial virus (rsv) bronchiolitis by measuring inflammatory mediators in peripheral blood and, if found, whether these related to the severity of illness. | 1997 | 9135260 |
| detection of respiratory syncytial virus from clinical specimens: comparison between reverse transcription polymerase chain reaction and tissue culture. | rt-pcr was compared with tissue culture to detect rsv from nasopharyngeal aspirates. rt-pcr was more sensitive and specific than tissue culture method. rt-pcr has sensitivity and specificity of 100% and 97%, respectively. the results indicate that rt-pcr can be used for detection of rsv in clinical specimens. | 1995 | 9139376 |
| nucleotide sequence of the genes encoding the matrix and small hydrophobic proteins of pneumonia virus of mice. | the nucleotide sequences of the genes encoding the matrix (m) protein and the small hydrophobic (sh) protein of pneumonia virus of mice (pvm) are described. the matrix protein gene contains a large open reading frame encoding polypeptide of 257 residues which shows considerable (38.6-42.1%) amino acid identity with the matrix proteins of other pneumoviruses. the matrix gene also contains a second, smaller, open reading frame (orf) as seen with the equivalent genes of other pneumoviruses. the pvm ... | 1997 | 9140191 |
| phosphoprotein profile analysis of ruminant respiratory syncytial virus isolates. | to determine the apparent molecular weight for 24 ruminant respiratory syncytial viruses (rsv) on the basis of differences in the electrophoretic mobility of the phosphoprotein (p protein). | 1997 | 9140554 |
| subgroup specific protection of mice from respiratory syncytial virus infection with peptides encompassing the amino acid region 174-187 from the g glycoprotein: the role of cysteinyl residues in protection. | we identified subgroup specific protective epitopes represented by the amino acid regions 174-187 and 171-187 of the g glycoproteins from respiratory syncytial virus (rsv), subgroups a and b. mice immunized with coupled synthetic peptides corresponding to either the region 174-187 containing a cys186-->ser substitution or to the native region 171-187 were completely resistant to rsv infection but only to the respective virus. the protective activities of the peptides 174-187 were dependent on th ... | 1997 | 9141214 |
| induction of protective immunity in rodents by vaccination with a prokaryotically expressed recombinant fusion protein containing a respiratory syncytial virus g protein fragment. | a subunit approach to the development of a respiratory syncytial virus (rsv) vaccine was investigated. it involved the production, in escherichia coli, of an rsv (long) g protein fragment (g2na) as a c-terminal fusion partner to an albumin binding region (bb) of streptococcal protein g. g2na incorporated amino acid residues 130-230 and was specifically recognized by murine anti-rsv-a polyclonal serum. in mice, intraperitoneal immunization with bbg2na induced high anti-rsv-a serum elisa titers an ... | 1997 | 9143271 |
| antigenically distinct g glycoproteins of brsv strains share a high degree of genetic homogeneity. | bovine respiratory syncytial (brs) virus can be divided into antigenic subgroups based on the reactivity of monoclonal antibodies (mabs) to the attachment glycoprotein, g. further, the polyclonal antibody response of calves vaccinated with recombinant vaccinia viruses expressing the g protein of a particular subgroup is also subgroup-specific. to investigate the genetic basis for the antigenic heterogeneity of the brs virus g protein, the genes for the g protein from 6 brs virus strains represen ... | 1997 | 9143302 |