Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| evidence for deficiencies in intracerebral cytokine production, adhesion molecule induction, and t cell recruitment in herpes simplex virus type-2 infected mice. | we examined the intracerebral t cell response in mice infected with neurovirulent hsv-2 strains and an avirulent hsv-1. in hsv-2-infected brains, (i) il-1beta, tnf-alpha and ifn-gamma mrna expression was low, (ii) icam-1 and vcam-1 were not induced, (iii) few cd4+ or cd8+ cells were detected. by contrast, in hsv-1-infected brains, (i) cytokine mrna expression was high, (ii) adhesion molecules were strongly expressed, (iii) many t cells were detected. we suggest that deficient t cell extravasatio ... | 1998 | 9521606 |
| evidence that deficient ifn-gamma production is a biological basis of herpes simplex virus type-2 neurovirulence. | although immune response control of herpes simplex virus (hsv) has been well demonstrated, numerous hsv-2 strains are neurovirulent in immunocompetent mice. using an rnase protection assay and an elisa, we found that hsv-2-infected mice exhibited a deficient ifn-gamma response, an inability to clear virus, and eventual death. an hsv-based amplicon vector expressing mouse ifn-gamma was constructed and packaged into hsv-1-helper virus (hsv(pifn-gamma)). in mice treated with hsv(pifn-gamma), (i) th ... | 1998 | 9521607 |
| neonatal herpes simplex virus infections. | neonatal herpes simplex virus (hsv) infection is one of the life-threatening infections of newborns. it affects approximately 1,500 to 2,200 infants per year in the united states. changes in the presentation of neonatal hsv infection over the past two decades include an increase in the frequency of skin, eye, and mouth (sem) disease with a relatively unchanged rate of central nervous system (cns) disease, but a relative decline in disseminated infection. although the mortality of neonatal hsv in ... | 1998 | 9523400 |
| perspectives on switching oral acyclovir from prescription to over-the-counter status: report of a consensus panel. | the proposed switching of oral acyclovir from prescription to over-the-counter (otc) status for the 5-day episodic treatment of genital herpes was considered by a consensus panel. it was concluded that self-diagnosis/misdiagnosis, misuse, and adverse drug effects were potential problems with the otc use of acyclovir. while acyclovir reduces asymptomatic shedding of herpes simplex virus type 2, the reduction in transmission of virus potentially resulting from increased acyclovir use was felt to b ... | 1998 | 9524840 |
| mucosal immunity to herpes simplex virus type 2 infection in the mouse vagina is impaired by in vivo depletion of t lymphocytes. | intravaginal (ivag) inoculation of wild-type herpes simplex virus type 2 (hsv-2) in mice causes epithelial infection followed by lethal neurological illness, while ivag inoculation of attenuated hsv-2 causes epithelial infection followed by development of protective immunity against subsequent ivag challenge with wild-type virus. the role of t cells in this immunity was studied by in vivo depletion of these cells with monoclonal antibodies. three groups of mice were used for each experiment: non ... | 1998 | 9525585 |
| the quantity of latent viral dna correlates with the relative rates at which herpes simplex virus types 1 and 2 cause recurrent genital herpes outbreaks. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) have evolved specific anatomic tropisms and site-dependent rates of reactivation. to determine whether reactivation rates depend on distinct abilities of hsv-1 and -2 to establish latency and to express latency-associated transcripts (lats), virulent strains of each virus were studied in the guinea pig genital model. following infection with equivalent titers of virus, the quantities of latent hsv-2 genomes and lats were higher in lumbosacral ... | 1998 | 9525595 |
| clearance of hsv-2 from recurrent genital lesions correlates with infiltration of hsv-specific cytotoxic t lymphocytes. | the mechanisms involved in host clearance of symptomatic mucocutaneous herpes simplex virus (hsv) infection are unclear. we studied the functional properties of bulk cultures of skin-infiltrating lymphocytes from normal skin and serial biopsies of recurrent genital hsv-2 lesions, and compared hsv-specific and nk responses with viral clearance. hsv-specific cd4+ or cd8+ t cells were rarely detected in lymphocytes cultured from normal skin. the total lymphocyte count and hsv-specific and nk-like e ... | 1998 | 9525993 |
| the novel immunosuppressive agent mycophenolate mofetil markedly potentiates the antiherpesvirus activities of acyclovir, ganciclovir, and penciclovir in vitro and in vivo. | the immunosuppressive agent mycophenolate mofetil (mmf) has been approved for use in kidney transplant recipients and may thus be used concomitantly for the treatment of intercurrent herpesvirus infections with drugs such as acyclovir (acv), ganciclovir (gcv), and penciclovir (pcv). we found that mmf and its parent compound mycophenolic acid (at concentrations that are attainable in plasma) strongly potentiate the antiherpesvirus (herpes simplex virus [hsv] type 1 [hsv-1], hsv-2, thymidine kinas ... | 1998 | 9527762 |
| influence of mucosal and parenteral immunization with a replication-defective mutant of hsv-2 on immune responses and protection from genital challenge. | herpes simplex virus (hsv) most frequently initiates infection at a mucosal surface; thus mucosal immune responses are likely to be important in defense against hsv infection. we have examined the effects of eliciting mucosal as well as systemic immune responses on protection against genital challenge infection with virulent hsv-2 in mice immunized with a replication-defective mutant of hsv-2. in addition, we have examined the types of immune responses elicited by immunization by the different r ... | 1998 | 9527927 |
| the comparative biology of latent herpes simplex virus type 1 and type 2 infections: latency-associated transcript promoter activity and expression in vitro and in infected mice. | hsv-1 and hsv-2 express abundant latency-associated transcripts (lats) without which these viruses reactivate in animals inefficiently. to further characterize the importance of lats to the comparative biology of latent hsv-1 and -2 infections, we assessed the relative activities of the viral lat promoters in vitro using transient transfection assays, and the accumulation of lats in vivo using a mouse ocular infection model. in vitro, the hsv-2 lat promoter proved to be six to tenfold more poten ... | 1998 | 9531009 |
| increase in vdelta1+ gammadelta t cells in the peripheral blood and bone marrow as a selective feature of hiv-1 but not other virus infections. | dysregulation of t-cell receptor (tcr) alphabeta bearing lymphocytes and an increase in vdelta1+ gammadelta t cells are typical features of hiv-1 infection. however, the role of gammadelta t cells remains unclear. therefore, peripheral blood mononuclear cells (pbmc) of 103 hiv-1-infected patients were investigated with respect to expression of vdelta1. these results were compared to the vdelta1 expression of bone marrow mononuclear cells (bmmc). in contrast to healthy controls, vdelta1+ cells do ... | 1998 | 9531341 |
| [genital herpes with special emphasis on perinatal herpes simplex virus infection]. | the incidence of genital herpes is increasing worldwide and at present herpes simplex virus type 2 is the most common cause of genital ulceration all over the world. classification: the international herpes management forum (ihmf) was established in 1993, suggesting a new classification of genital herpes: primary genital herpes, non-primary genital herpes, recurrent genital herpes, first episode genital herpes, atypical genital herpes and asymptomatic hsv (herpes simplex virus) infection. | 1998 | 9531774 |
| the management of herpes simplex virus infection in pregnancy. | 1998 | 9532983 | |
| evidence of latency and reactivation of both herpes simplex virus (hsv)-1 and hsv-2 in the genital region. | while superinfection with different herpes simplex virus (hsv) types has been demonstrated in animals, the ability of the two hsv types to colonize and reactivate in the same anatomic region in humans has not been well demonstrated. in 6 patients, both hsv-1 and hsv-2 was recovered from genital lesions. in 4 of them, who initially acquired genital hsv-1 infection, subsequent hsv-2 infection presented as a prolonged episode of genital lesions and a marked increase in the frequency of genital recu ... | 1998 | 9534984 |
| antibodies to varicella zoster virus in the cerebrospinal fluid of neonates with seizures. | four neonates with convulsions had igg antibodies in their cerebrospinal fluid (csf) to varicella zoster virus (vzv). these antibodies were found in the sera of two of these patients after the age of 6 months. antibodies to 16 different microbes were studied from the serum and csf of 201 neonates with neurological problems. the presence of dna specific to hsv-1, hsv-2, and vzv in the csf was also investigated using the polymerase chain reaction (pcr). antibodies to vzv were detected in the csf o ... | 1998 | 9536843 |
| genital herpes: epidemiology and pathophysiology. update and new perspectives. | genital herpes simplex virus (hsv) is a sexually transmitted disease (std) which affects millions of people worldwide and is mainly due to hsv type 2. seroprevalence rates as high as 60-90% have been reported in developing countries. in developed countries, 20% of the general population is hsv2 seropositive. recent epidemiological surveys employing type-specific antibody assays show that the prevalence of hsv-2 infections is rising at an alarming rate. also, the epidemiology is changing with an ... | 1997 | 9538399 |
| a study of the viral etiology of histiocytic necrotizing lymphadenitis (kikuchi-fujimoto disease). | histiocytic necrotizing lymphadenitis (hnl) or kikuchi's disease is a distinctive, self-limited disorder characterized by necrotizing cervical lymphadenopathy in young individuals. hnl is more prevalent among asians and is a relatively common disorder among koreans. a preceding fever, lymphopenia, and occasional skin rashes suggest a viral etiology and there have been sporadic reports of viral association. however, so far, no infectious agent has been proven to be etiologically related. in the p ... | 1998 | 9539315 |
| metal complexes of bovine lactoferrin inhibit in vitro replication of herpes simplex virus type 1 and 2. | the inhibitory effect of bovine lactoferrin (blf) saturated with ferric, manganese or zinc ions, on the infection of vero cells by human herpes simplex virus type 1 (hsv1) and 2 (hsv2) was investigated. viral infectivity determined by intracellular antigen synthesis and plaque formation was efficiently inhibited by metal saturated lactoferrins in a dose-dependent manner. effective blf concentrations which reduced the infection by 50% ranged from 5.2 to 31 micrograms ml-1 and were far below the c ... | 1998 | 9542061 |
| tipping the scales of herpes simplex virus reactivation: the important responses are local. | 1998 | 9546773 | |
| an in vitro assessment of the antineoplastic potential of 2h-1,3-oxazine-2,6(3h)-dione (3-oxauracil), a novel pyrimidine. | the pyrimidine (uracil) analogue 3-oxauracil (ou) previously had been shown to completely inhibit the growth of e. coli b and decrease by 96% the replication of herpes simplex virus type 2 when present in the culture fluid at a concentration of 10(2) microm. limited in vivo studies in mice demonstrated antiviral effects without significant toxicity when given i.p. daily for two weeks at a concentration of 3.23 mg/kg. however, the antineoplastic properties of ou were unknown. we assessed the abil ... | 1997 | 9547671 |
| herpes simplex virus and human papillomavirus in a population-based case-control study of cervical intraepithelial neoplasia grade ii-iii. | in order to evaluate the association between seropositivity for herpes simplex virus (type 1 and type 2) and cervical intraepithelial neoplacia (cin), we analysed data from a population-based case-control study of cin grade ii-iii which included norwegian women aged 20 to 44 years, 94 cases and 228 controls. our objectives were to determine if hsv-1 and/or hsv-2 seropositivity were independent risk factors for cin, taking human papillomavirus exposure into account, and to elucidate the combined ... | 1998 | 9548432 |
| fulminant hepatitis due to herpes simplex virus-type 2 in early phase of bone marrow transplantation. | hepatitis due to herpes simplex virus (hsv) is a rare and severe infection in patients with impaired immunity, as bone marrow transplanted. the antemortem diagnosis is often difficult to establish because the clinical features are nonspecific. we report an uncommon cause of fulminant hepatic failure in a neutropenic patient, 14 days after bone marrow transplantation. hsv-2 fulminant hepatitis occurred during acyclovir prophylactic treatment. no observation of hsv hepatitis in this context has be ... | 1998 | 9556188 |
| transport of viruses through fetal membranes: an in vitro model of perinatal transmission. | a model system for perinatal transmission of viral infections was developed and transport of infectious virus particles through fetal membranes was investigated. viruses of different families known to cause serious intrauterine infections were selected, including relevant and model viruses: the dna-viruses hsv-1 and -2 as well as the animal herpes viruses bhv-1 and shv-1, the rna-virus bvdv as a model for hepatitis c virus, hiv-1 and -2, and ppv as a model for parvovirus b19. migration of infect ... | 1998 | 9557298 |
| t-lymphocytopaenia, opportunistic infections and pathological findings in ghanaian aids patients and their sexual partners. | ninety-nine patients at center for disease control (cdc) clinical stage iv were studied. twelve (12.12%) of these patients turned out to be hiv seronegative. ten out of the 12 hiv negative patients were immunocompetent whereas the other two had proportional decreases in both cd4+ and cd8+ t-lymphocytes. hiv-1, hiv-2, and dual infection, were detected in 51.5%, 2%, and 22.2% respectively of clinical aids patients. the other 12.12% of clinical aids patients were indeterminate for hiv antibodies. a ... | 1997 | 9557423 |
| herpes simplex virus 2 ul45 is a type ii membrane protein. | in addition to eleven glycoproteins, the herpes simplex virus type 2 (hsv-2) genome encodes several proteins with potential membrane-spanning segments but no asparagine-linked carbohydrates. one of these is ul45. fractionation of infected cells showed that hsv-2 ul45 is an integral membrane protein, and analysis of ul45 mutants with potential glycosylation sites showed that it has a type ii membrane orientation, the first hsv protein known to have this orientation. furthermore, it is detectable ... | 1998 | 9557736 |
| infrequent detection of cytomegalovirus and epstein-barr virus dna in synovial membrane of patients with rheumatoid arthritis. | to study the role of the cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus types 1 and 2 (hsv-1 and 2), varicella zoster virus (vzv), and human herpes virus 6 (hhv-6) in the etiology of rheumatoid arthritis (ra). | 1998 | 9558160 |
| site-directed mutagenesis probing the catalytic role of arginines 165 and 166 of human cytomegalovirus protease. | human cytomegalovirus (cmv) is a member of the herpesviridae family of viruses that also includes herpes simplex viruses (hsv-1 and hsv-2), varicella-zoster virus (vzv), human herpes virus-6, 7, and 8 (hhv-6, hhv-7, and hhv-8), and epstein-barr virus (ebv). each member of this family encodes a serine protease that is a potential target for antiviral therapeutic intervention. we recently reported the crystal structure of cmv proteases [qiu, x., culp, j. s., dilella, a. g., hellmig, b., hoog, s. s ... | 1998 | 9558326 |
| thymoma and cellular immune deficiency in an adolescent. | thymoma has been associated with both humoral immunodeficiency and cellular immunodeficiency, but the latter association has never been described in the pediatric age group. we report a 15-year-old female with thymoma, recalcitrant oropharyngeal candidiasis, recurrent generalized cutaneous herpes simplex virus type 2 infection, recurrent pneumonia and myasthenia gravis. pathology of the thymic lesion showed a 10x5x6 cm extensively hyalinized mass with residual regions of spindle cell predominant ... | 1998 | 9560844 |
| prevalence and correlates of herpes simplex virus type 2 infection: evaluation of behavioural risk factors. | to examine the prevalence and correlates of infection with herpes simplex virus type 2 (hsv-2) among sexually transmitted disease (std) clinic attenders, we studied the prevalence of antibodies to hsv-2 and their association with risk behaviour. | 1998 | 9563706 |
| the high-risk sexual practices of crack-smoking sex workers recruited from the streets of three american cities. the multicenter crack cocaine and hiv infection study team. | small ethnographic and clinic-based studies indicate that crack-smoking sex workers are at high risk for human immunodeficiency virus (hiv) and sexually transmitted diseases (std). study goals: to examine the prevalence of risky sexual behaviors and hiv and std in a large sample of street-recruited crack-smoking sex workers. | 1998 | 9564720 |
| characterization of the protein encoded by gene ul49a of herpes simplex virus type 1. | herpes simplex virus type 1 (hsv-1) gene ul49a potentially encodes a primary translation product of 91 residues with a signal sequence at the n terminus and a membrane anchor domain near the c terminus. mutants were generated in this gene and utilized to characterize the encoded protein on sds-page as a 6.7 kda species which fractionated with infected cell membranes, was a relatively abundant virion component, and was not detectably o-glycosylated. the protein was identified by microsequencing a ... | 1998 | 9568977 |
| migration of lymphoid cells from vaginal epithelium to iliac lymph nodes in relation to vaginal infection by herpes simplex virus type 2. | to determine whether lymphocytes and langerhans cells in vaginal epithelium are migratory, we stained mouse vaginal epithelium, including its lymphoid cells, by intraluminal administration of h33342, a fluorescent, vital dye. stromal staining was superficial, and no free dye reached the iliac lymph nodes. the numbers and phenotypes of h33342-stained cells that migrated from the vagina to the iliac lymph nodes during the next 48 h were determined in four groups: normal mice, mice infected intrava ... | 1998 | 9570531 |
| genital recurrent infection occurring 6 months after meningitis due to the same herpes simplex virus type 2 (hsv-2) strain evidence by restriction endonuclease analysis. | herpes simplex virus type 2 (hsv-2) is more often sexually transmitted and associated with genital recurrent infection. however, hsv-2 neurological manifestations such as meningitis were already reported. we describe a case of meningitis due to hsv-2, preceded by signs suggesting a common cystitis, in a woman with no history of primary or recurrent genital infection. six months later genital herpetic lesions occurred. one hsv-2 strain was obtained from cerebrospinal fluid (csf) and another from ... | 1998 | 9570665 |
| subcellular localization of the us3 protein kinase of herpes simplex virus type 2. | one supposes that herpes simplex virus us3 gene product possessing serine/threonine protein kinase activity is a cytoplasmic enzyme. to determine its subcellular localization during viral replication we prepared an antiserum to a synthetic oligopeptide corresponding to the n-terminal region of the us3 protein of hsv type 2 strain 186. the us3 protein first appeared in the cytoplasm of infected cell at 4 h postinfection but strong fluorescence was detected in the nuclei at 8 h postinfection. at 1 ... | 1998 | 9572561 |
| inhibition of major histocompatibility complex class i antigen presentation in pig and primate cells by herpes simplex virus type 1 and 2 icp47. | herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) express an immediate-early protein, icp47, that effectively inhibits the human transporter associated with antigen presentation (tap), blocking major histocompatibility complex (mhc) class i antigen presentation to cd8+ t cells. previous work indicated that the mouse tap is relatively resistant to inhibition by the hsv-1 and hsv-2 icp47 proteins (icp47-1 and icp47-2) and that mouse cells infected with hsv-1 are lysed by anti-hsv cd8+ cytotoxic ... | 1998 | 9573278 |
| immunoglobulin g, plasma cells, and lymphocytes in the murine vagina after vaginal or parenteral immunization with attenuated herpes simplex virus type 2. | this investigation evaluated immunity to vaginal herpes simplex virus type 2 (hsv-2) infection after local or parenteral immunization with attenuated hsv-2. vaginal immunization induced sterilizing immunity against challenge with a high dose of wild-type virus, whereas parenteral immunizations protected against neurologic disease but did not entirely prevent infection of the vagina. vaginal immunization caused 86- and 31-fold increases in the numbers of immunoglobulin g (igg) plasma cells in the ... | 1998 | 9573285 |
| [hsv-1 is also involved]. | 1998 | 9577069 | |
| meningoencephalitis: an atypical presentation of herpes simplex type 2 central nervous system infection. | encephalitis is the best recognized form of central nervous system (cns) infection with the herpes simplex virus. we present a case of meningoencephalitis with a benign course caused by herpes simplex virus type 2. the patient had no focal abnormalities on either brain magnetic resonance imaging scan or electroencephalogram. the cerebrospinal fluid profile was that of aseptic meningitis, with a lymphocytic pleocytosis. the clinical spectrum of herpes simplex infections in the cns is broad. speci ... | 1998 | 9584606 |
| [impact of focal allergic test with herpetic vaccine in diagnosis of ophthalmic herpes]. | clinical and immunological studies were carried out in 697 patients (913 eyes) for a more accurate diagnosis of herpetic involvement of the posterior segment of the eye by the focal allergic test (fat) based on the delayed type hypersensitivity phenomenon. fat consists in detection of the focal reaction in the eye, regularly developing after 2-3 intracutaneous injections of herpetic vaccine to types i and ii herpes simplex virus in a dose of 0.2 ml every other day. the reaction is assessed by cl ... | 1998 | 9584632 |
| synthesis of nucleosides having unusual branched sugars as potential antiviral agents. | enantiomerically pure novel nucleosides having unusual branched sugars were synthesized in a stereospecific manner from a common chiral pool of (s, s)-1,4-bis(benzyloxy)-2,3-epoxybutane and evaluated for antiviral activity. | 1997 | 9586000 |
| a serosurvey of haemophilus ducreyi, syphilis, and herpes simplex virus type 2 and their association with human immunodeficiency virus among female sex workers in lagos, nigeria. | to determine the prevalence rates of serological reactivity of haemophilus (h.) ducreyi, treponema pallidum, and herpes simplex virus type 2 (hsv-2) antibodies among female sex workers (fsws) and their association with human immunodeficiency virus (hiv) antibody status. | 1998 | 9587174 |
| evaluation of a live attenuated recombinant virus rav 9395 as a herpes simplex virus type 2 vaccine in guinea pigs. | recombinant virus rav 9395 was constructed by deleting both copies of the gamma(1)34.5 gene, and the ul55 and ul56 open reading frames from herpes simplex virus type 2 (hsv-2) strain g. the potential use of rav 9395 as an hsv-2 vaccine was investigated by evaluating the ability of rav 9395 to protect guinea pigs from severe disease by hsv-2(g) challenge. rav 9395 administered intramuscularly reduced both lesion development and severity in a dose-dependent manner in guinea pigs challenged with hs ... | 1998 | 9592996 |
| long-term immunity and protection against herpes simplex virus type 2 in the murine female genital tract after mucosal but not systemic immunization. | the degree and duration of immunity against herpes simplex virus type 2 (hsv-2) infection of the female genital tract were assessed after intranasal (i.nl.) or intraperitoneal (i.p.) immunization with a recombinant adenovirus vector expressing hsv glycoprotein b (adgb8). after intravaginal hsv-2 challenge, control mice rapidly developed disease and displayed high virus titers in vaginal washes. in contrast, virus titers decreased significantly and at similar rates in i.nl. and i.p. immunized mic ... | 1998 | 9592997 |
| foscarnet decreases hiv-1 plasma load. | to evaluate the effect of foscarnet on hiv-1 replication in vivo. | 1998 | 9593457 |
| an epidemiologic study of herpes simplex virus type 1 and 2 infection in japan based on type-specific serological assays. | a seroepidemiologic study of herpes simplex virus type 1 (hsv-1) and 2 (hsv-2) was performed on japanese adults. serum samples collected between 1985-9 from a total of 536 healthy adults, female prostitutes, males with sexually transmitted diseases (std), homosexual men, and pregnant women were studied by immunodot assays using hsv type-specific antigens, glycoproteins g (gg1 and gg2). hsv-1 infections correlated mostly with age and was widely prevalent among subjects < 40 years. hsv-2 prevalenc ... | 1998 | 9593488 |
| [molecular cloning and sequence analysis of glycoprotein d gene of herpes simplex virus 2 strain sav and wild strain isolated]. | to study the difference of structure and function of hsv-2 gd gene among the wild strain, g and sav strain. so as to provide theoretical basis for future production of hsv-2 vaccine. | 1997 | 9596937 |
| a comparison of pcr with virus isolation and direct antigen detection for diagnosis and typing of genital herpes. | patients attending the genitourinary medicine clinic at watford general hospital, uk, were examined for clinical signs of genital herpes infection. genital swabs were taken from 194 patients (126 female, 68 male) who presented with genital ulceration or symptoms which were suggestive of genital herpes infection. swabs from these patients were tested by three methods: (i) detection of herpes simplex virus (hsv) antigen by direct hsv enzyme immunoassay (eia), (ii) hsv isolation in vero cell cultur ... | 1998 | 9598940 |
| an indolent penile herpetic ulcer in a patient with previously undiagnosed human immunodeficiency virus infection. | we report a case of an atypical penile herpetic ulcer in a man with previously undiagnosed human immunodeficiency virus (hiv) infection. swabs of the lesion were negative for herpes simplex virus (hsv) by culture and electron microscopy. however, histopathology of biopsy material showed characteristic herpetic multinucleate giant cells. immunochemistry using polyclonal antibodies was positive for hsv1 and hsv2. the lesion completely resolved after treatment with aciclovir and the patient subsequ ... | 1998 | 9602886 |
| localization of type-specific epitopes of herpes simplex virus type 2 glycoprotein g recognized by human and mouse antibodies. | glycoprotein g is a major target for the humoral immune response against herpes simplex virus (hsv) and a prototype antigen for type-specific serodiagnosis discriminating hsv-1 and hsv-2 infections. the mature part of hsv-2 glycoprotein g-2 (gg-2) contains a unique stretch suspected to mediate type specificity, and in addition a region homologous to hsv-1 glycoprotein g-1 (gg-1). antigenic determinants of the mature gg-2 were mapped by testing the reactivity of mouse anti-gg-2 monoclonal antibod ... | 1998 | 9603337 |
| herpes simplex virus myelitis: clinical manifestations and diagnosis by the polymerase chain reaction method. | herpes simplex virus (hsv) myelitis has previously been reported to be a form of acute ascending necrotizing myelitis caused by hsv type 2 (hsv-2). we studied neurological symptoms, clinical course, magnetic resonance imaging (mri) findings, and diagnosis by polymerase chain reaction (pcr) methods in 9 patients with hsv myelitis. in 6 cases, disease onset was marked by sensorimotor disturbances of lower extremities and urinary disturbances, with the transverse myelopathy gradually ascending to t ... | 1998 | 9605393 |
| does the latency associated transcript (lat) of herpes simplex virus (hsv) function as a ribozyme during viral reactivation? | the latency associated transcript (lat) of herpes simplex virus (hsv) appears to exist as an rna molecule only. this phenomenon is consistent with the concept of functioning at an rna level, and several lines of evidence suggest that the lat may be a ribozyme. this provides an insight into understanding the role of lat during hsv reactivation. | 1998 | 9608658 |
| serologic profile of some sexually transmitted diseases in women with squamous intraepithelial lesions. | the purpose of this study consisted of the evaluation of some sexually transmitted diseases in patients with cervical pathology, namely squamous intraepithelial lesions. | 1998 | 9611052 |
| activity of n-chlorotaurine against herpes simplex- and adenoviruses. | n-chlorotaurine, an essential weak oxidant produced by stimulated human leukocytes, is known to have bactericidal, fungicidal and vermicidal properties. this study for the first time demonstrates its virucidal activity. by viral suspension tests at incubation times between 5 and 60 min, virus titers of both herpes simplex virus type 1 and 2 were reduced about 1.3-2.9 log10 and 2.8-4.2 log10 by 0.1 and 1%, (5.5 and 55 mm) n-chlorotaurine, respectively. virus titer reduction of adenovirus type 5 b ... | 1998 | 9614001 |
| superior cytostatic activity of the ganciclovir elaidic acid ester due to the prolonged intracellular retention of ganciclovir anabolites in herpes simplex virus type 1 thymidine kinase gene-transfected tumor cells. | ganciclovir (gcv) and its lipophilic elaidic acid ester prodrug e-gcv were evaluated for their antiherpetic, cytostatic and metabolic properties, e-gcv proved exquisitely inhibitory to the replication of herpes simplex virus type 1 (hsv-1) and hsv-2 in cell cultures (50% effective concentration (ec50): 0.002 microm). it was five- to 10-fold more effective than its parent drug gcv. e-gcv was at least 2000-fold more cytostatic to hsv-1 or hsv-2 thymidine kinase (tk) gene-transfected mammary carcin ... | 1998 | 9614564 |
| entry of alphaherpesviruses mediated by poliovirus receptor-related protein 1 and poliovirus receptor. | a human member of the immunoglobulin superfamily was shown to mediate entry of several alphaherpesviruses, including herpes simplex viruses (hsv) 1 and 2, porcine pseudorabies virus (prv), and bovine herpesvirus 1 (bhv-1). this membrane glycoprotein is poliovirus receptor-related protein 1 (prr1), designated here as hvec. incubation of hsv-1 with a secreted form of hvec inhibited subsequent infection of a variety of cell lines, suggesting that hvec interacts directly with the virus. poliovirus r ... | 1998 | 9616127 |
| immunity to vaginal infection by herpes simplex virus type 2 in adult mice: characterization of the immunoglobulins in vaginal mucus. | progestin-treated female mice are susceptible to vaginal infection by two sexually transmitted disease organisms: herpes simplex virus type 2 (hsv-2) and chlamydia trachomatis. vaccination of mice with hsv-2 or chlamydial antigens elicits immunity to vaginal infection that may be due in part to secreted antibodies in the vaginal lumen. analysis of the role of these antibodies in immunity would be aided by information about the vaginal secretion in progestin-treated mice and the antibodies it con ... | 1998 | 9616875 |
| a therapeutic vaccine that reduces recurrent herpes simplex virus type 1 corneal disease. | to investigate the therapeutic efficacy of periocular vaccination with herpes simplex virus (hsv) recombinant glycoprotein d from hsv-1 (gd1) or hsv-2 (gd2) in decreasing hsv-induced recurrent dendritic keratitis and hsv-induced recurrent ocular shedding in rabbits latently infected with hsv-1. | 1998 | 9620075 |
| ul27.5 is a novel gamma2 gene antisense to the herpes simplex virus 1 gene encoding glycoprotein b. | an antibody made against the herpes simplex virus 1 us5 gene predicted to encode glycoprotein j was found to react strongly with two proteins, one with an apparent mr of 23,000 and mapping in the s component and one with a herpes simplex virus protein with an apparent mr of 43,000. the antibody also reacted with herpes simplex virus type 2 proteins forming several bands with apparent mrs ranging from 43,000 to 50,000. mapping studies based on intertypic recombinants, analyses of deletion mutants ... | 1998 | 9621069 |
| prevalence of herpes simplex virus infection in patients suspected of genital herpes; and virus typing by type specific fluorescent monoclonal antibodies. | during the period between april 1994 and february 1996, a total of 154 female patients who attended the clinic of female sexually transmitted diseases, siriraj hospital with clinical symptoms suspected of genital herpes were investigated for herpes simplex virus (hsv) infection by the virus isolation method in vero cell cultures. swabs from external genital lesions and the cervix from each patient were collected separately and used as the clinical specimens for isolation of hsv. the virus isolat ... | 1998 | 9623019 |
| to treat or not to treat? | 1998 | 9623969 | |
| predicting and preventing the emergence of antiviral drug resistance in hsv-2. | genital herpes, caused by herpes simplex virus, is the most prevalent sexually transmitted disease worldwide. in many developing countries genital herpes is untreated, and in the united states only 10% of cases are treated. we present a mathematical model that we use as a health policy tool to predict the levels of antiviral drug resistance that would emerge, if treatment rates were increased, and to identify the key factors in determining the emergence of drug resistance. we use our results to ... | 1998 | 9623975 |
| relationship of stable integration of herpes simplex virus-2 bg/ii n subfragment xho2 to malignant transformation of human papillomavirus-immortalized cervical keratinocytes. | transfection of the right end xho2 subfragment of bg/ii n of herpes simplex virus-2 (hsv-2) into human genital keratinocytes immortalized by human papillomavirus (hpv) type 16 or 18 resulted in invasive and noninvasive indolent cystic squamous carcinomas when cells were injected into immunocompromised mice. retention and expression of the right end portion of the bg/ii n fragment correlated with malignancy, as the corresponding hsv-2 sequences were integrated and transcribed in the tumorigenic c ... | 1998 | 9626354 |
| a nested-pcr assay for the simultaneous amplification of hsv-1, hsv-2, and hcmv genomes in patients with presumed herpetic cns infections. | to facilitate early diagnosis of herpes virus infection of the central nervous system (cns), a nested polymerase chain reaction (npcr) assay was developed to test simultaneously for the presence of hsv-1, hsv-2, and hcmv dna in the cerebrospinal fluid (csf) of patients with herpetic cns disease suspected on clinical grounds. the virus type-specific pcr products were differentiated either by agarose gel electrophoresis or by dna enzyme immunoassay. using titrated viral stocks as standards, a sens ... | 1998 | 9628226 |
| slow release devices: std protection at last? | 1996 | 9630916 | |
| controlled release of antibodies for long-term topical passive immunoprotection of female mice against genital herpes. | current methods for sexually transmitted diseases (std) prophylaxis, which can be disruptive and inconvenient, must be used before each act of sexual intercourse, so a method that provides protection over the course of many acts is desirable. we used a mouse model of vaginally-transmitted herpes simplex virus 2 (hsv-2) infection to test polymeric controlled-release devices for sustained passive immunoprotection. vaginal disks were prepared by dispersing a monoclonal antibody to hsv-2 (iii-174) w ... | 1996 | 9630921 |
| improved adenovirus vector provides herpes simplex virus ribonucleotide reductase r1 and r2 subunits very efficiently. | we have constructed a new adenovirus (ad) expression vector, padbm5, that allows for the production of unprecedented levels of recombinant protein in the human 293 cell line using the ad expression system. the main feature of this vector is a combination of enhancer sequences that increases the activity of the ectopic major late promoter (mlp) in recombinant ad. in 293 cells infected with helper-free ad recombinants generated with the padbm5 transfer vector, both herpes simplex virus (hsv) ribon ... | 1995 | 9634800 |
| rapid assay of phage-derived recombinant human fabs as bispecific antibodies. | specific anti-tumor and anti-viral activities can be conferred on lymphocytic and myeloid effector cells by retargeting them with bispecific antibodies. these are antibodies which possess an anti-target binding region and a region capable of binding specific effector cell surface markers. for the rapid evaluation of recombinant human fabs as bispecific antibodies, we have constructed a vector that allows for the conversion of fabs into protein a fusion proteins. these can be used to generate bis ... | 1995 | 9636296 |
| t lymphocytes are required for protection of the vaginal mucosae and sensory ganglia of immune mice against reinfection with herpes simplex virus type 2. | intravaginal inoculation of mice with an attenuated strain of herpes simplex virus type 2 (hsv-2) resulted in vigorous hsv-specific immune responses that protected against subsequent challenge with fully virulent hsv-2 strains. even in the presence of high titers of hsv-specific ab, t cell-dependent mechanisms were required for protection of the vaginal mucosae of hsv-immune mice and could be detected by 24 h after intravaginal reinoculation. depletion of specific t cell subsets from hsv-immune ... | 1998 | 9637526 |
| dendritic cells internalize vaccine adjuvant after intramuscular injection. | vaccine adjuvants help antigens elicit rapid, potent, and long-lasting immune responses. the lack of understanding of the immunological mechanism of action of adjuvants has limited the rational development of vaccines for human use. in particular, little is known about how the immune system processes adjuvants. the goal of the present study was to determine the fate of the vaccine adjuvant mf59, labeled with the fluorescent dye dil, after injection with fluorescein-labeled gd2 antigen from type ... | 1998 | 9637761 |
| isolation of a herpes simplex virus type 1-like agent from the brain of a mountain agile gibbon (hylobates agilis agilis) with encephalitis. | a captive-born 2-yr-old male mountain agile gibbon (hylobates agilis agilis) that died of encephalitis harbored a herpes simplex virus type 1 (hsv1)-like agent in the brain. a complete necropsy revealed intensive meningeal congestion with nonsuppurative encephalitis. the virus was recovered from frozen brain tissue in vero cells. the isolate was very similar but not identical to human hsv1. both western blot assay and virus neutralization tests were done with sera from 15 gibbons. antibodies aga ... | 1998 | 9638628 |
| [detecting pathogens of fetal defects in paraffin embedded tissues by polymerase chain reaction]. | to examine 3 types of pathogens in dead fetus with congenital defects for exploring the pathogenesis. | 1997 | 9639727 |
| [significance of antibodies to herpesviridae viruses detectable in rheumatic diseases]. | to assay antibodies to cytomegalovirus (cmv), herpes simplex virus type 1 and 2 (hsv-1, hsv-2) and epstein-barr virus (ebv) in rheumatic patients and to clarify clinical correlations. | 1998 | 9644741 |
| characterization of the ul16 gene product of herpes simplex virus type 2. | we have raised rabbit polyclonal antisera against a his-tagged herpes simplex virus type 1 (hsv-1) ul16 fusion protein, one of which very specifically reacted with 40 kda and 41 kda proteins in the lysates of hsv-1 and hsv-2-infected cells, respectively. since its reactivity to the 41 kda protein was clearly eliminated by pre-adsorption with e. coli lysates expressing the ul16 fusion protein, the antiserum was used to characterize the ul16 products of hsv-2. the hsv-2 ul16 protein was produced a ... | 1998 | 9645194 |
| t cell recognition motifs of an immunodominant peptide of myelin basic protein in patients with multiple sclerosis: structural requirements and clinical implications. | myelin basic protein (mbp)-reactive t cells may play an important role in the pathogenesis of multiple sclerosis (ms). the t cell response to the 83-99 region of mbp represents a dominant autoreactive response to mbp in ms patients of dr2 haplotype. in this study, a large panel of dr2- and dr4-restricted t cell clones specific for the mbp83-99 peptide were examined for the recognition motifs and structural requirements for antigen recognition using alanine-substituted peptides. our study reveale ... | 1998 | 9645371 |
| rapid detection, culture-amplification and typing of herpes simplex viruses by enzyme immunoassay in clinical samples. | the laboratory diagnosis of herpes simplex infection may require rapid (direct) tests, as well as cell cultures, for detection of the virus in clinical samples. the quantity of virus present in clinical samples is variable and this may depend on the period from onset of rash. in addition, not all patients may show obvious symptoms with this infection. the successful culture of herpes simplex virus requires prompt transportation after collection of the specimen as the virus is easily inactivated. ... | 1998 | 9646003 |
| cervical antibody responses to a herpes simplex virus type 2 glycoprotein subunit vaccine. | effective vaccines against genital herpes simplex virus type 2 (hsv-2) may need to induce genital tract immune responses. to determine local antibody responses to hsv-2 glycoproteins gb2 and gd2 in an intramuscular subunit vaccine, cervical secretions from hsv-seronegative women and hsv-1-seropositive women were tested for igg and iga to gb2 and gd2 by enhanced chemiluminescence western blot. most (94%) of the seronegative subjects developed cervical igg to gb2, igg to gd2, and iga to gb2; 72% d ... | 1998 | 9652416 |
| limited variability of glycoprotein gene sequences and neutralizing targets in herpes simplex virus type 2 isolates and stability on passage in cell culture. | nucleotide sequence analyses of polymerase chain reaction-amplified genes were performed to determine whether adaptation of herpes simplex virus type 2 to replication in cultured cells or in internal organs during neonatal disseminated disease results in selection of variants with altered forms of three glycoproteins (gb, gc, or gd) that influence virus entry into cells. no variations in sequence were noted as a consequence of in vitro passage or replication in different organs. five viruses fro ... | 1998 | 9652417 |
| prevalence of transfusion associated infections in multitransfused children in relation to mandatory screening of hiv in donated blood. | any change in risk behavior related to acquisition of human immunodeficiency virus (hiv) infection is likely to reduce simultaneously the risk for other agents transmitted through identical routes. a study carried out in the city of delhi, india on the load of transfusion associated infections among multitransfused (mt) children in relation to mandatory screening of hiv infection in donated blood indicated unchanged prevalence of hepatitis b virus (hbv), hepatitis c virus (hcv) and hepatitis d v ... | 1997 | 9656388 |
| a cell surface protein with herpesvirus entry activity (hveb) confers susceptibility to infection by mutants of herpes simplex virus type 1, herpes simplex virus type 2, and pseudorabies virus. | certain mutant strains of herpes simplex virus type 1 (hsv-1) are unable to infect cells in which entry is dependent on hvem, the previously described herpesvirus entry mediator designated here as herpesvirus entry protein a (hvea). these mutant viruses can infect other cells where entry is apparently dependent on other co-receptors. the mutant virus hsv-1(kos)rid1 was used to screen a human cdna expression library for ability of transfected plasmids to convert resistant chinese hamster ovary ce ... | 1998 | 9657005 |
| intranasal immunization of mice with herpes simplex virus type 2 recombinant gd2: the effect of adjuvants on mucosal and serum antibody responses. | mucosal immunization offers the potential for inducing iga antibody responses in the vagina, the site of infection for many viruses, including herpes simplex type 2 (hsv-2). to investigate this possibility, mice were immunized intranasally with 10 micrograms glycoprotein d2 (gd2) from hsv combined with a series of adjuvants of proven efficacy; the oil in water emulsion mf59, poly(d,l-lactide-co-glycolide) microparticles (plg) (encapsulated or co-administered), immune-stimulating complexes (iscom ... | 1998 | 9659230 |
| frequent recovery of hiv-1 from genital herpes simplex virus lesions in hiv-1-infected men. | genital ulcer disease has been epidemiologically linked as a risk factor in the transmission of the human immunodeficiency virus 1 (hiv-1). while herpes simplex virus 2 (hsv-2) is the most common cause of genital ulcers, no study has systematically evaluated the frequency or titer of hiv-1 virus in hsv-2 lesions. | 1998 | 9660365 |
| antiherpesvirus activities of (1's,2'r)-9-[[1',2'-bis(hydroxymethyl)cycloprop-1'-yl]methyl]guanine (a-5021) in cell culture. | antiherpetic activity of (1's,2'r)-9-([1',2'-bis(hydroxymethyl)cycloprop-1'yl]methyl)guanine (a-5021) was compared with those of acyclovir (acv) and penciclovir (pcv) in cell cultures. in a plaque reduction assay using a selection of human cells, a-5021 showed the most potent activity in all cells. against clinical isolates of herpes simplex virus type 1 (hsv-1, n = 5) and type 2 (hsv-2, n = 6), mean 50% inhibitory concentrations (ic50s) for a-5021 were 0.013 and 0.15 microgram/ml, respectively, ... | 1998 | 9661001 |
| prevalence of papillomavirus, epstein-barr virus, cytomegalovirus, and herpes simplex virus type 2 in urinary bladder cancer. | recent epidemiological studies suggest that the risk for urological malignancies may be related to the exposure to infectious agents. human papillomaviruses type 16 and 18 (hpv 16, hpv 18), epstein-barr virus (ebv), cytomegalovirus (cmv) and herpes simplex virus type 2 (hsv-2) have been suggested previously as cofactors in the pathogenesis of some malignancies in humans. the present paper, the presence of hpv 16, hpv 18, ebv, cmv and hsv-2 genomes was investigated in a panel of 35 biopsies from ... | 1998 | 9661833 |
| mapping of linear antigenic determinants on glycoprotein c of herpes simplex virus type 1 and type 2 recognized by human serum immunoglobulin g antibodies. | using membrane-based dekapeptides, the reactivity of human serum antibodies with linear antigenic determinants of herpes simplex virus (hsv) type 1 and type 2 glycoprotein c (gc-1, gc-2) was studied by pep scan and immunodot assay. the entire coding sequences of gc-1 and gc-2 were screened for the presence of linear epitopes by pep scan. peptides recognized in an hsv-1 type-specific manner were mainly identified within the n-terminal third and at the c-terminus of gc-1, whereas most type-common ... | 1998 | 9661836 |
| surveys of hiv-1, htlv-i, and other sexually transmitted diseases in female sex workers in taipei city, taiwan, from 1993 to 1996. | between 1993 and 1996 in taipei, taiwan, we studied the prevalences of hiv-1, htlv-i, hsv-2, chlamydia trachomatis, and syphilis infection in female commercial sex workers (csw) and the pathogens' associated risk factors. female csw from nine massage parlors, two karaoke bars (kb), and 22 brothels in taipei city participated in this study. two of 557 (0.4%) kb csws from those who participated during the period from 1993 to 1994 had hiv-1 infection, whereas no kb csws in the periods 1994 to 1995 ... | 1998 | 9665510 |
| rapid screening tests for determining in vitro susceptibility of herpes simplex virus clinical isolates. | the susceptibility of human herpes simplex virus (hsv) to acyclovir (acv) was determined with the use of a single dose of the drug (1 and 2 micrograms of acv per ml for hsv-1 and hsv-2, respectively) in two rapid assays: a rapid cytopathic effect inhibitory assay (rapid cia) and a rapid dye uptake assay (rapid dua). these tests allow the simultaneous determination of virus titer and susceptibility to acv at a determined viral concentration (100 50% tissue culture infective doses and 100 50% dye ... | 1998 | 9666034 |
| dna increases the potency of vaccination against infectious diseases. | in the past couple of years, the idea that naked dna can be used to vaccinate against infections has been rapidly developing. in contrast to traditional protein or live attenuated vaccines, there is no risk of disease caused by dna vaccines as only selected proteins are encoded. the ease with which dna may be manipulated means that vaccines can be custom designed to meet many needs. in animal model systems, dna vaccines have proved to be as effective as traditional vaccines. additionally, this t ... | 1997 | 9667856 |
| low-productive alpha-herpesviridae infection in chronic lichenoid dermatoses. | herpes simplex virus (hsv) and varicella-zoster virus (vzv) are responsible for various atypical mucocutaneous manifestations in the immunosuppressed population. one of the causative pathomechanisms suggests an altered virus-host cell relationship. | 1998 | 9669126 |
| the effect of gramicidin, a topical contraceptive and antimicrobial agent with anti-hiv activity, against herpes simplex viruses type 1 and 2 in vitro. | the effect of an anti-hiv compound, gramicidin, previously used as a topical antibiotic and vaginal contraceptive, on the replication of herpes simplex viruses (hsv) type 1 and 2 has been examined. human wi-38 fibroblasts were inoculated with either hsv type in the presence of serial dilutions of gramicidin and reduction in viral yield was measured by elisa. the 50% inhibitory dose (ic50) of gramicidin against 3 hsv-1 and 4 hsv-2 isolates was equal to 0.3 microgram/ml and was comparable to the e ... | 1997 | 9672588 |
| characterization of t cell responses to herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) using a tnf-beta elispot cytokine assay. | we examined the suitability of a tnf-beta cytokine elispot assay for assessing various aspects of the t cell response to herpes simplex viruses. the number of t cells responding to hsv-1 or hsv-2 was measured by tnf-beta elispot assay. the number of t cells producing tnf-beta in response to hsv-1 was high, ranging from 76 to 222 per 10(5). hsv-1-specific tnf-beta-secreting responder cell frequencies fluctuated over time in individual donors. comparable fluctuations were not observed in the t cel ... | 1997 | 9672626 |
| nonoxynol-9 enhances rectal infection by herpes simplex virus in mice. | pretreating the rectum of mice with nonoxynol-9 (n9) or spermicides containing n9 before infecting via the rectal route with herpes simplex virus 2 (hsv-2) increased the likelihood of infection and shortened the time until infection. microscopic analysis indicated that n9 rapidly caused exfoliation of the rectal epithelium, exposing the underlying connective tissue. these findings suggest that use of n9-containing products during rectal intercourse may increase the risk of infection with hsv-2 o ... | 1998 | 9673842 |
| a review of famciclovir in the management of genital herpes. | the frequent occurrence of genital herpes continues to be a serious clinical problem. although not life threatening, the physical symptoms of the disease, and the ensuing psychosocial complications, can be overwhelming to patients. the life cycle of the herpes simplex virus is complex, comprising multiple stages. following infection, the virus establishes life-long latency in its host and can reactivate at any time as a recurrent infection. successful management of genital herpes simplex infecti ... | 1998 | 9678146 |
| functional analysis of the herpes simplex virus type 2 strain hg52 rl1 gene: the intron plays no role in virulence. | sequence analysis predicts that herpes simplex virus type 2 (hsv-2) strain hg52 contains an open reading frame, rl1, encoding a polypeptide equivalent to icp34.5 of hsv-1. similarly to hsv-1, deletion of the region spanning rl1 abolishes the virulence of hsv-2 strain hg52 and its ability to grow in stationary 3t6 cells. in contrast to hsv-1, the hsv-2 strain hg52 rl1 gene is predicted to contain a 154 bp intron. previously, we have demonstrated that this intron is spliced from rl1 poly(a)+ mrna ... | 1998 | 9680122 |
| interferon alpha (ifn alpha 2a) therapy for herpes virus-associated inflammatory bowel disease (ulcerative colitis and crohn's disease). | the etiology and pathogenesis of ulcerative colitis and crohn's disease remain unclear, so that exact causal therapy is not yet possible. in our uc and cd patients, viral infections, particularly of the upper respiratory tract, aggravated the underlying disease. this had led us to use in-situ hybridization to investigate intestinal mucosa for viral agents such as hsv i + ii- and epstein-barr virus dna. we found these dna in the cell nuclei in the surface and glandular epithelia of the affected m ... | 1998 | 9684118 |
| antiviral activities of methylated nordihydroguaiaretic acids. 2. targeting herpes simplex virus replication by the mutation insensitive transcription inhibitor tetra-o-methyl-ndga. | we had previously reported that tetramethyl-o-ngda (m4n), a synthetic derivative of the naturally occurring nordihydroguaiaretic acid (ndga), is able to inhibit hiv tat transactivation by blocking host sp1 protein at the sp1 cognate binding site on the hiv ltr promoter. the present studies were undertaken to examine whether m4n is able to inhibit the replication of herpes simplex virus (hsv), another sp1-regulated virus. the results showed that in vero cells, m4n inhibits at micromolar levels (i ... | 1998 | 9685239 |
| [monoclonal antibodies which recognize herpes simplex virus and their possible diagnostic application]. | it is reported the obtention of monoclonal antibodies, which are capable of recognizing hsv-1 and hsv-2 viruses, by the conventional technology of cellular fusion. the monoclonal antibodies of highest positivity according to the indirect immunofluorescence technique were partially characterized. it was determined the neutralizing capacity of 2 of them (278-f7 and 70-g10), which were used to identify isolations of clinical specimens by indirect immunofluorescence. these monoclonal antibodies reco ... | 1997 | 9685985 |
| mode of action of (1's,2'r)-9-[[1',2'-bis(hydroxymethyl) cycloprop-1'-yl]methyl]guanine (a-5021) against herpes simplex virus type 1 and type 2 and varicella-zoster virus. | the mode of action of (1's,2'r)-9-([1', 2'-bis(hydroxymethyl)cycloprop-1'-yl]methyl)guanine (a-5021) against herpes simplex virus type 1 (hsv-1), hsv-2, and varicella-zoster virus (vzv) was studied. a-5021 was monophosphorylated at the 2' site by viral thymidine kinases (tks). the 50% inhibitory values for thymidine phosphorylation of a-5021 by hsv-1 tk and hsv-2 tk were comparable to those for penciclovir (pcv) and lower than those for acyclovir (acv). of these three agents, a-5021 inhibited vz ... | 1998 | 9687413 |
| characterization of the ul4 gene product of herpes simplex virus type 2. | we have identified the herpes simplex virus type 2 (hsv-2) ul4 gene product using a rabbit polyclonal antiserum raised against a recombinant 6xhis-ul4 fusion protein expressed in escherichia coli. the antiserum reacted specifically with a 27-kda protein in hsv-2 186-infected cell lysates. the protein was not detectable in the presence of the viral dna synthesis inhibitor, suggesting that the ul4 gene was expressed as a gamma 2 gene. indirect immunofluorescence studies localized the ul4 protein w ... | 1998 | 9687876 |
| inhibition of herpes simplex virus replication by a 2-amino thiazole via interactions with the helicase component of the ul5-ul8-ul52 complex. | with the use of a high-throughput biochemical dna helicase assay as a screen, t157602, a 2-amino thiazole compound, was identified as a specific inhibitor of herpes simplex virus (hsv) dna replication. t157602 inhibited reversibly the helicase activity of the hsv ul5-ul8-ul52 (ul5/8/52) helicase-primase complex with an ic50 (concentration of compound that yields 50% inhibition) of 5 microm. t157602 inhibited specifically the ul5/8/52 helicase and not several other helicases. the primase activity ... | 1998 | 9696789 |
| recognition of herpes simplex virus type 2 tegument proteins by cd4 t cells infiltrating human genital herpes lesions. | the local cellular immune response to herpes simplex virus (hsv) is important in the control of recurrent hsv infection. the antiviral functions of infiltrating cd4-bearing t cells may include cytotoxicity, inhibition of viral growth, lymphokine secretion, and support of humoral and cd8 responses. the antigens recognized by many hsv-specific cd4 t cells localizing to genital hsv-2 lesions are unknown. t cells recognizing antigens encoded within map units 0. 67 to 0.73 of hsv dna are frequently r ... | 1998 | 9696844 |