Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| characterization and expression of a novel frizzled 9 gene in schistosoma japonicum. | wnt signaling regulates a diverse array of eukaryotic development processes, which are mediated by the frizzled family receptors. however, the role of this signaling pathway in the development of schistosoma japonicum remains poorly understood. we isolated a novel s. japonicum frizzled member (sjfz9), which encodes a 923 amino acid protein, sharing the general feature of frizzled proteins. we investigated its mrna and protein expression patterns during different life stages in definitive hosts. ... | 2011 | 21272670 |
| the ribosomal intergenic spacer (igs) region in schistosoma japonicum: structure and comparisons with related species. | the intergenic spacer (igs) between the 28s and 18s ribosomal rna genes was pcr-amplified, sequenced and characterized for schistosoma japonicum from mainland china, and compared with those of other schistosoma species. excluding flanking portions of the 28s and 18s genes, the igs in the longest sequenced amplicon from s. japonicum igs was 1457bp in length. however, intra-specific and intra-individual variation was noted. the igs region of s. japonicum is strikingly different in structure from t ... | 2011 | 21277395 |
| field detection of schistosoma japonicum cercariae in environmental water samples by quantitative pcr. | a species-specific quantitative pcr (qpcr) assay was combined with two novel water-sampling methods and compared with the mouse bioassay for the quantitative detection of s. japonicum in surface waters. the novel methods were capable of capturing cercariae and, with subsequent analysis through qpcr, detecting the presence of a minimum of 1 cercaria. | 2011 | 21278276 |
| oriental schistosomiasis with neurological complications: case report. | we describe a case of cerebral schistosomiasis, caused by asian (oriental) schistosoma japonicum trematode blood flukes, in a young chinese patient and its management. we also provide a brief update for physicians on the clinical features, diagnosis and treatment of schistosomiasis, with particular emphasis on neuroschistosomiasis, the most severe clinical outcome associated with this parasitic infection. | 2011 | 21294922 |
| variability in intron sequences of housekeeping and antigen-coding genes among schistosoma japonicum isolates in mainland china. | the accurate characterization of schistosoma japonicum has important implications for analyzing genetic variation and would provide basic data for disease control. previous studies using proteins, coding sequences, and especially antigen-coding genes showed lower genetic variation among s. japonicum isolates from mainland china. therefore, the present study focused on variations in intron sequences of housekeeping and antigen-coding genes, which may be more informative for genetic analysis. we c ... | 2011 | 21296680 |
| polymorphic microsatellites in the human bloodfluke, schistosoma japonicum, identified using a genomic resource. | re-emergence of schistosomiasis in regions of china where control programs have ceased requires development of molecular-genetic tools to track gene flow and assess genetic diversity of schistosoma populations. we identified many microsatellite loci in the draft genome of schistosoma japonicum using defined search criteria and selected a subset for further analysis. from an initial panel of 50 loci, 20 new microsatellites were selected for eventual optimization and application to a panel of worm ... | 2011 | 21299863 |
| [construction and expression of recombinant plasmid pet28alpha-sj26gst of schistosoma japonicum in escherichia coli bl21]. | to construct and express recombinant plasmid pet28a-sj26gst of schistosoma japonicum (sj) in escherichia coli bl21 (de3). | 2010 | 21302435 |
| artificial preparation, indoor passage, and nature breed of oncomelania hupensis infected with schistosoma japonicum. | to prepare the infected oncomelania hupensis by artificial method for the research on the activity, vaccine, and genetic variation of schistosoma japonicum (s. japonicum). | 2011 | 21311132 |
| a survey of schistosome protein domain types: insights into unique biological properties. | using the prosite database and search tools, we conducted a comprehensive bioinformatic analysis of the predicted protein sequences of the flatworm parasites schistosoma mansoni and schistosoma japonicum and seven other animal genomes in order to identify novel schistosome-specific features. our analyses revealed a relative paucity of proline-rich domains in schistosomes in comparison with their human host and a corresponding enrichment in schistosomes of asparagine-rich, serine-rich, and threon ... | 2011 | 21315771 |
| transdermal evaporation delivery system of praziquantel for schistosomiasis japonicum chemotherapy. | a transdermal evaporation delivery system (teds) of praziquantel (pzq) was developed by selecting ethylene glycol monophenyl ether as a nonvolatile component solvent and ethanol as a volatile component solvent to control efficiently the transmission and morbidity of the global schistosomiasis, providing a convenient administration system of pzq for both humans and domestic animals. the solubility of pzq in teds was more than 400 mg/ml when the ethanol concentration was 50% (w/w) in the solvent m ... | 2011 | 21319162 |
| molecular cloning, characterization, and immunolocalization of two lactate dehydrogenase homologous genes from taenia solium. | two novel genes encoding lactate dehydrogenase a (ldha) and b (ldhb) homologues, respectively, were identified from the cdna libraries of adult taenia solium (t. solium). the two deduced amino acid sequences both show more than 50% identity to the homologues for danio rerio, xenopus laevis, schistosoma japonicum, sus scrofa, homo sapiens, et al. the identity of the amino acid sequence between tsldha and tsldhb is 57.4%, and that of the nucleotide sequence is 61.5%. recombinant tsldha homologue ( ... | 2011 | 21331787 |
| a coproantigen diagnostic test for strongyloides infection. | accurate diagnosis of infection with the parasite strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. in this manuscript we describe the development of a coproantigen elisa for diagnosis of infection. polyclonal rabbit antiserum was raised against strongyloides ratti excretory/secreto ... | 2011 | 21347447 |
| spatially explicit agent-based modelling for schistosomiasis transmission: human-environment interaction simulation and control strategy assessment. | as the transmission of many other parasitic diseases, the transmission of schistosomiasis is a complex process governed by natural, socio-economic factors and human life style. based on the life cycle of schistosoma japonicum, some models have been developed. however, the human-environment interaction, especially through agricultural activities, has not been explicitly modeled in previous efforts. | 2010 | 21352776 |
| quantifying quality of life and disability of patients with advanced schistosomiasis japonica. | the chinese government lists advanced schistosomiasis as a leading healthcare priority due to its serious health and economic impacts, yet it has not been included in the estimates of schistosomiasis burden in the global burden of disease (gbd) study. therefore, the quality of life and disability weight (dw) for the advanced cases of schistosomiasis japonica have to be taken into account in the re-estimation of burden of disease due to schistosomiasis. | 2011 | 21358814 |
| expression, characterization and therapeutic efficacy of chimeric fab of anti-idiotypic antibody np30 against schistosoma japonicum. | the murine monoclonal anti-idiotypic antibody np30 is a promising therapeutic antibody against schistosoma japonicum. however, the immunogenicity of murine np30 limits its further study and application in humans. here the chimeric fab of np30 (chfab-np30) comprising the variable regions of murine np30 and constant regions of human antibody was assembled. chfab-np30 was expressed and purified as a soluble and functional protein. administration of chfab-np30 in vivo increased the survival rate, re ... | 2011 | 21376700 |
| toll-like receptor (tlr) 2 and tlr4 deficiencies exert differential in vivo effects against schistosoma japonicum. | little is known about the functions of toll-like receptor 2 (tlr2) and tlr4 in innate/acquired responses to schistosoma japonicum. through in vivo study, the contributions of tlr2 and tlr4 to host immune responses during s. japonicum infection were investigated. early infection experiments showed higher protein and mrna levels of il-12, ifn-+¦ and il-4 in dermal tissues and retroauricular draining lymph nodes respectively in tlr2(-/-) mice on day four post-infection and opposite changes in tlr4( ... | 2011 | 21392041 |
| in-vivo activity of dihydroartemisinin against schistosoma japonicum. | 2011 | 21396254 | |
| evaluation of mammalian and intermediate host surveillance methods for detecting schistosomiasis reemergence in southwest china. | schistosomiasis has reemerged in china, threatening schistosomiasis elimination efforts. surveillance methods that can identify locations where schistosomiasis has reemerged are needed to prevent the further spread of infections. | 2011 | 21408127 |
| a 5-year longitudinal study of schistosomiasis transmission in shian village, the anning river valley, sichuan province, the peoples' republic of china. | schistosoma japonicum is a major public health concern in the peoples' republic of china (prc), with over one million people infected and another 50 million living in areas at risk of infection. based on ecological, environmental, population genetic and molecular factors, schistosomiasis transmission in prc can be categorised into four discrete ecosystems or transmission modes. it is predicted that the three gorges dam (tgd) will impact upon the transmission of schistosomiasis in the prc, with v ... | 2011 | 21429229 |
| vaccine potential of hemocyanin from oncomelania hupensis against schistosoma japonicum. | hemocyanin, a giant oxygen transport protein which is usually found in many arthropods and mollusks was isolated and purified from oncomelania hupensis. in this study, we showed that oncomelania hupensis hemocyanin (ohh) shared carbohydrate epitopes with different developmental stages of schistosoma japonicum (cercaria, schistosomulum, adult worm and egg) and exhibited serological cross-reaction with these stages of s. japonicum immune sera, which had a potential for use in diagnostic and therap ... | 2011 | 21440665 |
| establishment of a cerebral schistosomiasis experimental model in rabbits. | the present study aimed to establish a cerebral schistosomiasis model in rabbits, to provide a valuable tool for morphological analysis, clinical manifestation observation, as well as investigations into immunological reactions and pathogenesis of focal inflammatory reaction in neuroschistosomiasis (ns). | 2011 | 21441970 |
| discovery and molecular characterization of a bcl-2-regulated cell death pathway in schistosomes. | schistosomiasis is an infectious disease caused by parasites of the phylum platyhelminthe. here, we describe the identification and characterization of a bcl-2-regulated apoptosis pathway in schistosoma japonicum and s. mansoni. genomic, biochemical, and cell-based mechanistic studies provide evidence for a tripartite pathway, similar to that in humans including bh3-only proteins that are inhibited by prosurvival bcl-2-like molecules, and bax/bak-like proteins that facilitate mitochondrial outer ... | 2011 | 21444803 |
| in silico prediction of binding of promiscuous peptides to multiple mhc class-ii molecules identifies the th1 cell epitopes from secreted and transmembrane proteins of schistosoma japonicum in balb/c mice. | it has not so far been possible to identify rapidly and effectively the anti-schistosomiasis th cell epitopes that are capable of simulating ifn-? (interferon-gamma)-mediated th1-type protective immunity in response to radiation-attenuated schistosome cercaria. with the advance of the omics studies of schistosomes, an approach that used reverse vaccinology probably resolved the above problems. in this "proof-of-principle" study, first, we selected 31 secreted or transmembrane protein sequences s ... | 2011 | 21458582 |
| assessing the impact of misclassification error on an epidemiological association between two helminthic infections. | polyparasitism can lead to severe disability in endemic populations. yet, the association between soil-transmitted helminth (sth) and the cumulative incidence of schistosoma japonicum infection has not been described. the aim of this work was to quantify the effect of misclassification error, which occurs when less than 100% accurate tests are used, in sth and s. japonicum infection status on the estimation of this association. | 2011 | 21468317 |
| mammalian apoptosis in a parasitic worm. | 2011 | 21493866 | |
| global expression analysis revealed novel gender-specific gene expression features in the blood fluke parasite schistosoma japonicum. | schistosoma japonicum is one of the remarkable platyhelminths that are endemic in china and southeast asian countries. the parasite is dioecious and can reside inside the host for many years. rapid reproduction by producing large number of eggs and count-react host anti-parasite responses are the strategies that benefit long term survival of the parasite. praziquantel is currently the only drug that is effective against the worms. development of novel antiparasite reagents and immune-prevention ... | 2011 | 21494327 |
| [comparative evaluation of five test kits for antibody detection in schistosoma japonicum endemic areas of poyang lake region]. | to evaluate the efficacy of five diagnostic test kits for the detection of antibodies to schistosoma japonicum in endemic areas of poyang lake. | 2010 | 21500532 |
| development of a double antibody sandwich elisa assay for the diagnosis of angiostrongyliasis. | abstract abstractusing two monoclonal antibodies (mabs) against excretory/secretory (es) antigens of angiostrongylus cantonensis adult worms, a method of double antibody sandwich elisa for the detection of circulating antigens (cag) was established with the best experimental conditions regulated for performance. to evaluate the sensitivity, the cag in sera of rats (80) and mice (15) infected with a. cantonensis as well as cag in sera of clinical confirmed angiostrongyliasis patients (70) were de ... | 2011 | 21506824 |
| mapping the binding between the tetraspanin molecule (sjc23) of schistosoma japonicum and human non-immune igg. | schistosomal parasites can establish parasitization in a human host for decades; evasion of host immunorecognition including surface masking by acquisition of host serum components is one of the strategies explored by the parasites. parasite molecules anchored on the membrane are the main elements in the interaction. sjc23, a member of the tetraspanin (tsp) family of schistosoma japonicum, was previously found to be highly immunogenic and regarded as a vaccine candidate against schistosomiasis. ... | 2011 | 21533061 |
| electrophoretic detection of genetic variability among schistosoma japonicum isolates by sequence-related amplified polymorphism. | in the present study, sequence-related amplification polymorphism (srap) was utilized to study the genetic variability among schistosoma japonicum isolates from different provinces in china, using schistosoma mansoni from puerto rico for comparison. five out of ten tested srap primer combinations displayed significant polymorphisms among s. japonicum isolates from china, namely me2/em1, me4/em1, me4/em6, me5/em4 and me5/em5. analysis of the 61 s. japonicum samples from china with five srap prime ... | 2011 | 21538983 |
| [the protective effect of chronic schistosoma japonica infestation against sepsis in mice.] | objective:to preliminarily study the protective effect of chronic schistosoma japonica (sj) infestation against sepsis in mice and its mechanism. methods:balb/c male mice were used, and the experiment was divided into three parts. experiment 1: chronic sj infestation model was reproduced by sj cercaria inoculation through abdominal skin for 8 weeks. twenty mice were randomly grouped into normal group (n=10) and sj group (n=10). the levels of interleukins (il-4, il-10),tumor necrosis factor-α (tn ... | 2011 | 21549067 |
| cloning and characterization of a novel enzyme: tyrosine hydroxylase from schistosoma japonicum. | catecholamines, such as dopamine and noradrenaline, play important roles as neuromuscular transmitters and modulators in all parasitic helminthes, including schistosoma japonicum. s. japonicum tyrosine hydroxylase (sjth) was amplified by rapid amplification of cdna ends polymerase chain reaction that shows strong homology to schistosoma mansoni tyrosine hydroxylase, the enzyme that catalyzes the first and rate-limiting step in the biosynthesis of catecholamines. the sjth transcripts encoded the ... | 2011 | 21556690 |
| isolation and characterization of the first polymorphic microsatellite markers for schistosoma haematobium and their application in multiplex reactions of larval stages. | the ability of microsatellite loci to reveal genetic diversity within the trematode schistosoma haematobium is demonstrated for the first time. nine novel polymorphic microsatellite markers were isolated and their viability assessed on 36 s. haematobium adult worm individuals from three geographical populations. allelic diversity and gene diversity ranged from two to seven and from 0.29 to 0.76, respectively, suggesting high variability between individuals and between unrelated populations. thre ... | 2008 | 21585859 |
| proteomic analysis of schistosoma japonicum schistosomulum proteins that are differentially expressed among hosts differing in their susceptibility to the infection. | schistosomiasis is a tropical, parasitic disease affecting humans and several animal species. the aim of this study was to identify proteins involved in the growth and survival of the parasitic forms inside a host. schistosomula of schistosoma japonicum were isolated from three different hosts: the susceptible balb/c mice; the wistar rats, which have a considerably lower susceptibility; and the resistant reed vole, microtus fortis. soluble proteins of the schistosomula collected from the above t ... | 2011 | 21593212 |
| performance of a dipstick dye immunoassay for rapid screening of schistosoma japonicum infection in areas of low endemicity. | abstract: background: the dipstick dye immunoassay (ddia), recently commercially available in the people's republic of china (p.r. china), is a rapid and simple test to detect human antibodies against schistosoma japonicum. its performance and utility for screening schistosome infection in low endemic areas is little known. we therefore carried out a cross-sectional survey in seven villages with low endemicity of schistosomiasis in p.r. china and assessed the performance and utility of ddia for ... | 2011 | 21599944 |
| characterization and expression of a novel cystatin gene from schistosoma japonicum. | cystatins are a family of cysteine protease inhibitors that play a crucial role in the immune evasion from their host and in the adaptation to host defence. here, we isolated a full-length cdna sequence inferred to encode a novel cystatin gene from a blood fluke, schistosoma japonicum. the cdna, designated sjcystatin, comprised an open reading frame (orf) of 306 bp, and encoded 101 amino acids with a predicted molecular weight of 11.3 kda. this predicted protein shared a significant degree of se ... | 2011 | 21601634 |
| a novel liquid-phase piezoelectric immunosensor for detecting schistosoma japonicum circulating antigen. | aims: a new liquid-phase piezoelectric immunosensor (lp-peis), which can detect schistosoma japonicum (sj) circulating antigens (sjcag) quantificationally, was developed. methods: the igg antibodies were purified from the sera of rabbits which had been infected or immunized by sj and were immobilized on the surface of piezoelectric quartz crystal in lp-peis by staphylococcal protein a (spa). it was used to detect sjcag in sera of rabbits which had been infected by sj in order to acquire some opt ... | 2011 | 21609782 |
| detection of the circulating antigen 14-3-3 protein of schistosoma japonicum by time-resolved fluoroimmunoassay in rabbits. | abstract: background: schistosomiasis remains a major public health concern that afflicts millions of people worldwide. low levels of schistosoma infection require more sensitive diagnostic methods. in this study, a time-resolved fluoroimmunoassay (trfia) was developed for detecting the signal transduction protein 14-3-3, a circulating antigen of schistosoma japonicum. results: the detection limit of 14-3-3-trfia was 0.78 ng/ml, with a linear measurement range from 0.78 to 800 ng/ml. the average ... | 2011 | 21619661 |
| cimetidine enhances the protective effect of gst dna vaccine against schistosoma japonicum. | cimetidine (cim), a histamine-2-receptor antagonist, has a long history of safe use in gastric acid-mediated gastrointestinal disorders. in this study, we used cim, as an adjuvant, with pegfp-sj26 gst (the recombinant plasmid containing enhanced green fluorescent protein gene and the gene encoding 26kda glutathione s-transferase of schistosoma japonicum) dna vaccine to immunized mice and attempted to enhance the protective effect against s. japonicum. the results showed that the reduction rate o ... | 2011 | 21624364 |
| effects of artemether, artesunate and dihydroartemisinin administered orally at multiple doses or combination in treatment of mice infected with schistosoma japonicum. | artemether and artesunate, derivatives of the antimalarial artemisinin, as well as their main metabolite, dihydroartemisinin, all exhibit antischistosomal activities. the purpose of the current study was to compare the effects of artemether, artesunate and dihydroartemisinin administered orally at multiple doses or combination in treatment of mice infected with schistosoma japonicum. we carried out experiments with mice, infected with 40 cercariae of s. japonicum, and treated with artemether, ar ... | 2011 | 21626153 |
| the secretions products from invading cercariae of s. japonicum (0-3hrp) restrain mouse dendritic cells to mature. | schistosomiasis is a water-borne infection caused mainly by human schistosomes including schistosoma mansoni (s. mansoni) and schistosoma japonicum (s. japonicum). in the infected host, immune events in the skin appear to have an important initiating role in determining the type, and guiding the magnitude, of the ensuing acquired immune response. the previous studies of s. mansoni showed that dendritic cells (dcs) prime th2 response after activating with products released by schistosome larvae ( ... | 2011 | 21626155 |
| irap: an efficient retrotransposon-based electrophoretic technique for studying genetic variability among geographical isolates of schistosoma japonicum. | in the present study, a inter-retrotransposon-amplified polymorphism (irap) technique, based on retrotransposons, was used to examine genetic variability among schistosoma japonicum isolates from different provinces in mainland china. of the 15 primers screened, 5 produced highly reproducible irap patterns. using these primers, 54 discernible dna fragments were generated with 40 (74.07%) being polymorphic, indicating considerable genetic variation among the examined s. japonicum isolates. the pr ... | 2011 | 21626522 |
| new insight into the antifibrotic effects of praziquantel on mice in infection with schistosoma japonicum. | schistosomiasis is a parasitic disease infecting more than 200 million people in the world. although chemotherapy targeting on killing schistosomes is one of the main strategies in the disease control, there are few effective ways of dealing with liver fibrosis caused by the parasite infection in the chronic and advanced stages of schistosomiasis. for this reason, new strategies and prospective drugs, which exert antifibrotic effects, are urgently required. | 2011 | 21629648 |
| inconsistent protective efficacy and marked polymorphism limits the value of schistosoma japonicum tetraspanin-2 as a vaccine target. | schistosoma mansoni tetraspanin 2 (sm-tsp-2) has been shown to be strongly recognized by igg1 and igg3 antibodies from individuals putatively resistant to schistosome infection, but not chronically infected people, and to induce high levels of protection against challenge infection in the murine model of schistosomiasis. amplification by pcr of homologous sequences from male and female s. japonicum worms showed the presence of 7 different clusters or subclasses of s. japonicum tsp-2. we determin ... | 2011 | 21655308 |
| karyopherin alpha 2 (kpna2) is associated with the natural resistance to schistosoma japanicum infection in microtus fortis. | microtus fortis is a naturally vertebrate host resistant to schistosoma japonicum infection. in order to understand the molecular mechanism and identify the molecules related to the natural resistance to s. japanicum infection of m. fortis, we screened a gene pool named ge76 by expression cloning and proved it to have high anti-schistosomula effects in our previous work. in this study we identified a clone named ge76.44. we found that the conditioned medium of pcdna1.1-ge76.44 caused 14.0% schis ... | 2011 | 21658898 |
| multiple vaccinations with uv- attenuated cercariae in pig enhance protective immunity against schistosoma japonicum infection as compared to single vaccination. | abstract: | 2011 | 21663666 |
| association of hla and post-schistosomal hepatic disorder: a systematic review and meta-analysis. | several human genetic variants, hla antigens and alleles are reportedly linked to post-schistosomal hepatic disorder (pshd), but the results from these reports are highly inconclusive. in order to estimate overall associations between human genetic variants, hla antigens, hla alleles and pshd, we systematically reviewed and performed a meta-analysis of relevant studies in both post-schistosomal hepatic disorder and post-schistosomal non-hepatic disorder patients. pubmed, scopus, google scholar, ... | 2011 | 21664486 |
| differential expression of chemokine and matrix re-modelling genes is associated with contrasting schistosome-induced hepatopathology in murine models. | the pathological outcomes of schistosomiasis are largely dependent on the molecular and cellular mechanisms of the host immune response. in this study, we investigated the contribution of variations in host gene expression to the contrasting hepatic pathology observed between two inbred mouse strains following schistosoma japonicum infection. whole genome microarray analysis was employed in conjunction with histological and immunohistochemical analysis to define and compare the hepatic gene expr ... | 2011 | 21666794 |
| schistosome serine protease inhibitors: parasite defense or homeostasis? | serpins are a structurally conserved family of macromolecular inhibitors found in numerous biological systems. the completion and annotation of the genomes of schistosoma mansoni and schistosoma japonicum has enabled the identification by phylogenetic analysis of two major serpin clades. s. mansoni shows a greater multiplicity of serpin genes, perhaps reflecting adaptation to infection of a human host. putative targets of schistosome serpins can be predicted from the sequence of the reactive cen ... | 2011 | 21670886 |
| non-coding rnas in schistosomes: an unexplored world. | non-coding rnas (ncrnas) were recently given much higher attention due to technical advances in sequencing which expanded the characterization of transcriptomes in different organisms. ncrnas have different lengths (22 nt to >1,000 nt) and mechanisms of action that essentially comprise a sophisticated gene expression regulation network. recent publication of schistosome genomes and transcriptomes has increased the description and characterization of a large number of parasite genes. here we revi ... | 2011 | 21670887 |
| dynamics of sept4 expression in fibrotic livers of mice infected with schistosoma japonicum. | n order to investigate the dynamics of septin4 (sept4) expression and its function in the formation of fibrotic livers in mice infected with schistosoma japonicum, we constructed the mouse model of s. japonicum egg-induced liver fibrosis for 24 weeks. immunohistochemical staining, qrt-pcr and western blot were used to detect the expression of sept4 and +¦-smooth muscle actin (+¦-sma). we found sept4 localized in the perisinusoidal space where hepatic stellate cells (hscs) distribute in the perip ... | 2011 | 21679490 |
| effect of praziquantel prolonged administration on granuloma formation around schistosoma japonicum eggs in lung of sensitized mice. | schistosomiasis remains a major public health problem and it is an immune disease. the schistosome egg is the primary parasite factor responsible for the overt disease. the eggs release the soluble antigen, which induces intensive tissue reaction, a granulomatous reaction to the eggs. if granuloma formation could be suppressed, overt disease might not develop. praziquantel is an effective antischistosomal drug especially for adult worms. however, whether praziquantel has a suppressing effect on ... | 2011 | 21695570 |
| the effects of interleukin (il)-12 and il-4 deficiency on worm development and granuloma formation in schistosoma japonicum-infected mice. | cd4(+) t-helper (th) cell is widely recognized to be capable of influencing worm development and egg granuloma formation after schistosome infection. interleukin (il)-12 and il-4 play key roles in regulation of th cell differentiation. in the present study, we subcutaneously inoculated mice with hybridoma cells secreting monoclonal antibodies to neutralize il-12 and il-4 and explored the effects of il-12 and il-4 deficiency on the worm development and granuloma formation in mice infected with ce ... | 2011 | 21710350 |
| adoptive transfer of dcs isolated from helminth-infected mice enhanced t regulatory cell responses in airway allergic inflammation. | our and others' previous studies have shown that schistosoma japonicum (sj) infection can inhibit allergic reactions. moreover, we found that adoptive transfer of dendritic cells (dcs) from inhibited mice showed a similar inhibitory effect on allergy, suggesting a critical role of dcs in sj infected mediated inhibition of allergy. in the present study, we further examined the mechanism by which dcs contribute to inhibition of allergy. our results showed that dcs from sj-infected mice (sjdcs) pro ... | 2011 | 21711363 |
| [expression of vwf73 and vwf114 fragments of von willebrand factor a2 domain and their utilization in detecting adamts13 activity]. | to construct the expression vectors of vwf73 and vwf114 fragments of von willebrand factor (vwf) a2 domain, and to express glutathione s-transferase (gst) fusion proteins in e. coli, and to explore their values in measuring adamts13 activity as substrates. | 2011 | 21729605 |
| apoptosis governs the elimination of schistosoma japonicum from the non-permissive host microtus fortis. | the reed vole, microtus fortis, is the only known mammalian host in which schistosomes of schistosoma japonicum are unable to mature and cause significant pathogenesis. however, little is known about how schistosoma japonicum maturation (and, therefore, the development of schistosomiasis) is prevented in m. fortis. in the present study, the ultrastructure of 10 days post infection schistosomula from balb/c mice and m. fortis were first compared using scanning electron microscopy and transmission ... | 2011 | 21731652 |
| human schistosomiasis resistance to praziquantel in china: should we be worried? | the efficacy of praziquantel for the treatment of schistosoma japonicum in humans is reported from a cross-sectional survey conducted in 33 villages in sichuan province. infection prevalence was found to be 5.7% (185 infected of 3,269 tested) in a region where 44-73% prevalence was found 9 years before. collected miracidia were subjected to an in vitro test of praziquantel susceptibility. an effective concentration of praziquantel associated with 50% of miracidia changing shape was found between ... | 2011 | 21734129 |
| an integrated strategy for transmission control of schistosoma japonicum in a marshland area of china: findings from a five-year longitudinal survey and mathematical modeling. | in 2004 an aggressive plan was instituted aiming to achieve nationwide transmission control of schistosomiasis by 2015. here, we report a longitudinal study on the control of schistosomiasis in anhui province, china. using a mathematical model, we compared the effects of different control strategies implemented in the study area. during the 5-year study period, a 60.8% reduction in human prevalence was observed from 2005 (7.95%) to 2009 (3.1%), and snail infection decreased from 0.063% in 2005 t ... | 2011 | 21734130 |
| characterization and vaccination of two novel schistosoma japonicum genes screened from a cercaria cdna library. | two novel genes, sjcwl05 and sjcwl06, were harvested from screening of schistosoma japonicum (s. japonicum) cercaria cdna library by using pig sera vaccinated (vps) with s. japonicum immature egg ws-vaccine (s. japonicum iew). prokaryotic recombinant plasmids pgex-4t-1/sjcwl05 and pgex-4t-1/sjcwl06 were constructed to analyze their immunogenicity, which was confirmed by sds-page and western blotting. two eukaryotic recombinant plasmids, pcdna3/sjcwl05 and pcdna3/sjcwl06, were constructed, and th ... | 2011 | 21739313 |
| ultrastructural alterations of juvenile schistosoma japonicum harbored in mice following mefloquine administration. | the aim of the present study was to assess the ultrastructural alterations of juvenile schistosoma japonicum induced by mefloquine. mice infected with 14-day-old s. japonicum were treated orally with mefloquine at a single dose of 400 mg/kg. between 8 h and 7 days after treatment, groups of two mice were sacrificed, and schistosomula were recovered for transmission electron microscopic observations. ultrastructural damage was seen in the tegument, subtegumental musculature, parenchymal tissues, ... | 2011 | 21750873 |
| compensatory density feedback of oncomelania hupensis populations in two different environmental settings in china. | abstract: background: the most recent strategy for schistosomiasis control in the people's republic of china aims to reduce the likelihood of environmental contamination of schistosome eggs. despite considerable progress, it is believed that achievements would be further consolidated with additional intermediate host snail control measures. we provide an empirical framework for discerning the relative contribution of intrinsic effects (density feedback) from other extrinsic drivers of snail popu ... | 2011 | 21752244 |
| internal versus external determinants of schistosoma japonicum transmission in irrigated agricultural villages. | currently schistosomiasis transmission has been suppressed to low levels in many historically endemic areas of china by widespread use of praziquantel in human and bovine populations and application of niclosamide for snail control. however, re-emergent transmission has signalled the need for sustainable interventions beyond these repeated chemical interventions. to take advantage of ongoing investment in rural infrastructure, an index of schistosomiasis transmission potential is needed to ident ... | 2011 | 21752808 |
| curupira-1 and curupira-2, two novel mutator-like dna transposons from the genomes of human parasites schistosoma mansoni and schistosoma japonicum. | summarytransposons of the mutator superfamily have been widely described in plants, but only recently have metazoan organisms been shown to harbour them. in this work we describe novel mutator superfamily transposons from the genomes of the human parasites schistosoma mansoni and s. japonicum, which we name curupira-1 and curupira-2. curupira elements do not have terminal inverted repeats (tirs) at their extremities and generate target site duplications (tsds) of 9 base pairs. curupira-2 transpo ... | 2011 | 21756422 |
| three goose-type lysozymes in the gastropod oncomelania hupensis: cdna sequences and lytic activity of recombinant proteins. | three goose-type (g-type) lysozymes, designated as ohlysg1, ohlysg2 and ohlysg3 were identified from expressed sequence tags (ests) of a gastropod oncomelania hupensis, the intermediate host of schistosoma japonicum. the full cdna sequences of ohlysg1, ohlysg2 and ohlysg3 consisted of 735, 909 and 808 nucleotides, with an open reading frame of 198, 214 and 249 codons containing a 21, 7 and 8 amino acid (aa) signal peptide at the n-terminus, respectively. the three g-type lysozymes shared conserv ... | 2011 | 21756934 |
| a highly sensitive taqman real-time pcr assay for early detection of schistosoma species. | schistosomiasis is a major infectious disease and a public health concern in many areas in china and other countries. sensitive method for detection of the parasite is critical for early diagnosis and for monitoring of effective treatment of the disease. in this study, we developed a highly sensitive taqman real-time pcr assay for the detection of schistosoma japonicum dna in mouse feces and serum samples. this assay was based on the dna sequence of the s. japonicum 18s rrna gene and was able to ... | 2011 | 21763257 |
| cloning, expression, and characterization of schistosoma japonicum tegument protein phosphodiesterase-5. | the tegument proteins of schistosomes are regarded as potential vaccine candidates and drug targets to control schistosomiasis. nucleotide pyrophosphatase/phosphodiesterase-5 (npp-5), which belongs to a multigene family of nucleotide pyrophosphatase/phosphodiesterases (npps), is important in the hydrolysis of pyrophosphate or phosphodiester bonds in nucleotides and their derivatives. in the present study, sjnpp-5, identified as one of the tegument proteins of schistosoma japonicum in our previou ... | 2011 | 21779860 |
| molecular characterization of cathepsin b from clonorchis sinensis excretory/secretory products and assessment of its potential for serodiagnosis of clonorchiasis. | abstract: background: cathepsin cysteine proteases play multiple roles in the life cycle of parasites such as food uptake, immune invasion and pathogenesis, making them valuable targets for diagnostic assays, vaccines and drugs. the purpose of this study was to identify a cathepsin b of clonorchis sinensis (cscb) and to investigate its diagnostic value for human helminthiasis. results: the predicted amino acid sequence of the cathepsin b of c. sinensis shared 63%, 52%, 50% identity with that of ... | 2011 | 21794140 |
| effects of protein extract from head-foot tissue of oncomelania hupensis on the growth and gene expression of mother sporocysts of schistosoma japonicum. | oncomelania hupensis is the intermediate host of schistosoma japonicum. in the present study, we investigated the effects of protein extracts from head-foot or gland tissue of o. hupensis on mother sporocysts of s. japonicum cultured in vitro. in the presence of head-foot protein extract of snails from the native province hunan, in-vitro-transformed mother sporocysts presented not only a longer survival time and stronger motility, but also a bigger size than parasites cultured with protein extra ... | 2011 | 21800125 |
| [study on diagnosis of chronic schistosomiasis japonicum with rsj26-sj32 fusion protein]. | to study whether rsj26-sj32 fusion protein can be used for diagnosis of chronic schistosomiasis japonicum. | 2011 | 21806877 |
| worm morphology of schistosoma japonicum using confocal laser scanning microscopy. | male and female schistosoma japonicum worms have dissimilar appearances in their final host. in this study, a morphometric and morphological assessment of whole worms derived from unisexual and mixed infections in mice was conducted using confocal laser scanning microscopy. worms from mixed infections showed significant morphological changes between 15 and 25 days post-infection (pi). on the fifteenth day pi, 33% of males had formed the conspicuous gynecophoric canal, but only 8% of them had tes ... | 2011 | 21810283 |
| paeoniflorin attenuates schistosomiasis japonica-associated liver fibrosis through inhibiting alternative activation of macrophages. | summaryinterleukin (il)-13 and alternatively activated macrophages (aams) play an important role in liver granuloma and fibrosis of schistosomiasis. paeoniflorin (pae, c23h28o11) has been reported to have an anti-hepatic fibrosis effect in schistosomiasis; however, the mechanism has not been fully elucidated. in this study, we measured serum hyaluronic acid (ha) concentrations, liver granuloma diameter and volume density, fibrosis degree and expressions of il-13, arginase-1 (arg-1), nitric oxide ... | 2011 | 21810309 |
| differential gene expression in schistosoma japonicum schistosomula from wistar rats and balb/c mice. | abstract: | 2011 | 21819550 |
| [recombinant plasmid zlw/pegfp-c2 transfection into schistosomula of schistosoma japonicum]. | to study the efficiency of zlw/pegfp-c2 plasmid transfection into schistosoma japonicum schistosomula and observe its in vitro effect of anti-schistosomula. | 2011 | 21823317 |
| [detection of specific igg in the sera of patients with chronic schistosomiasis japonica by dot-elisa with the recombinant sj26-sj32 fusion protein]. | to study the diagnostic value of the dot elisa with rsj26-sj32 fusion protein for chronic schistosomiasis japonica. | 2011 | 21823318 |
| development of a rapid dipstick with latex immunochromatographic assay (dlia) for diagnosis of schistosomiasis japonica. | abstract: background: schistosomiasis japonica (schistosomiasis) is a zoonosis that can seriously affect human health. at present, the immunodiagnostic assays for schistosomiasis detection are time-consuming and require well-trained personnel and special instruments, which can limit their use in the field. thus, there is a pressing need for a simple and rapid immunoassay to screen patients on a large scale. in this study, we developed a novel rapid dipstick with latex immunochromatographic assay ... | 2011 | 21824443 |
| [cloning and expression of metalloprotease gene from schistosoma japoncum and its immunoprotective efficiency]. | to clone and express a metalloprotease gene of schistosoma japonicum, purify the expressed protein, and investigate the induced immune response in mice and its localization in the parasite. | 2011 | 21826888 |
| [preliminary function study on mago nashi gene of schistosoma japonicum]. | to study the function of mago nashi gene in reproductive system of schistosoma japonicumn. | 2011 | 21826889 |
| [ageing down-modulates the immune responses to schistosoma japonicum infection in mice]. | to investigate the effect of ageing on the immune responses against schistosoma japonicum infection in mice. | 2011 | 21826892 |
| [effect of ginsenoside rg3 on hepatic fibrosis in murine schistosomiasis japonica]. | to investigate the therapeutic efficacy of ginsenoside rg3 on hepatic fibrosis in murine schistosomiasis japonica. | 2011 | 21826894 |
| [diagnosis of chronic schistosomiasis japonicum with the recombinant sj26gst-sj32 fusion protein by elisa]. | to study the feasibility of rsj26gst-sj32-igg-elisa for diagnosis of chronic schistosomiasis japonicum. | 2011 | 21826993 |
| profiles of small non-coding rnas in schistosoma japonicum during development. | the gene regulation mechanism along the life cycle of the genus schistosoma is complex. small non-coding rnas (sncrnas) are essential post transcriptional gene regulation elements that affect gene expression and mrna stability. preliminary studies indicated that sncrnas in schistosomal parasites are generated through different pathways, which are developmentally regulated. however, the data of sncrnas of schistosomal parasites are still fragmental and a complete expression profile of sncrnas dur ... | 2011 | 21829742 |
| neuroschistosomiasis: clinical symptoms and pathogenesis. | neuroschistosomiasis, referring to schistosomal involvement of the cns, when symptomatic, is a severe disorder in which prognosis depends largely on early diagnosis and treatment. it is an underdiagnosed disorder, but has been increasingly reported in populations in endemic areas and in tourists. cns involvement can occur at any time during schistosomal infection. both the brain and the spinal cord can be affected. schistosoma mansoni and schistosoma haematobium usually cause myelopathy, whereas ... | 2011 | 21849166 |
| Further studies on mefloquine and praziquantel alone or interaction of both drugs against Schistosoma japonicum in vitro. | The aim of the present study is to further understand and analyze the interaction of mefloquine with praziquantel against adult Schistosoma japonicum in vitro. Mice infected with S. japonicum cercariae for 35-37 days were sacrificed, and adult schistosomes were collected by perfusion. Schistosomes were placed to each of 12 wells of a Falcon plate and maintained in RPMI 1640 supplemented by 10% calf serum. For determination of 50% and 95% lethal concentration (LC50 and LC95) of the two drugs in v ... | 2011 | 21853223 |
| genetic manipulation of schistosomes - progress with integration competent vectors. | summarydraft genome sequences for schistosoma japonicum and s. mansoni are now available. the schistosome genome encodes ∼13 000 protein-encoding genes for which the functions of few are well understood. nonetheless, the new genes represent potential intervention targets, and molecular tools are being developed to determine their importance. over the past 15 years, noteworthy progress has been achieved towards development of tools for gene manipulation and transgenesis of schistosomes. a brief h ... | 2011 | 21854676 |
| risk factors for helminth infections in a rural and a peri-urban setting of the dongting lake area, people's republic of china. | schistosomiasis japonica and soil-transmitted helminthiasis are endemic parasitic diseases in the people's republic of china (pr china). as very few studies have reported on the distribution and interaction of multiple species helminth infections, we carried out a comparative study of households in a rural village and a peri-urban setting in the dongting lake area of hunan province in november and december 2006 to determine the extent of single and multiple species infections, the underlying ris ... | 2011 | 21854780 |
| Schistosoma japonicum: treatment of different developmental stages in mice with long-acting praziquantel implants. | This paper reports the effective treatment of Schistosoma japonicum in a mouse model with long-acting praziquantel (PZQ)-loaded poly(e-caprolactone) implants. The implants yielded stable, high plasma PZQ concentrations ranging 100-1600 ng/mL during the 40-day investigation period. For assessment of efficacy, the implants were implanted into mice immediately after infection and at 1, 2, 3 and 4 weeks after infection to treat the schistosomes at different developmental stages. All the mice were sa ... | 2011 | 21856298 |
| impact of schistosoma japonicum infection on collagen-induced arthritis in dba/1 mice: a murine model of human rheumatoid arthritis. | the hygiene hypothesis suggests that helminth infections prevent a range of autoimmune diseases. | 2011 | 21858123 |
| Application of DNA-based diagnostics in detection of schistosomal DNA in early infection and after drug treatment. | Research is now focused on identification of sensitive and specific diagnostic tests for early identification of schistosomal infection and evaluation of chemotherapy in field situations in China. | 2011 | 21864384 |
| dihydroartemisinin-praziquantel combinations and multiple doses of dihydroartemisinin in the treatment of schistosoma japonicum in experimentally infected mice. | 2011 | 21871170 | |
| Molecular cloning and tissue distribution of a Schistosoma japonicum gene encoding AMY-1. | It is well known that the mammalian associate of Myc-1 (AMY-1) plays a significant role in spermatogenesis or cellular differentiation. A full-length complementary DNA (cDNA) encoding AMY-1 of Schistosoma japonicum (SjAMY-1) was identified and isolated from a cDNA library. The gene contained an open reading frame of 315 nucleotides, encoding 105 amino acids. Sequence analysis showed that SjAMY-1 shares 65.7% homology with Homo sapiens AMY-1 amino acids and contains a conserved domain from the AM ... | 2011 | 21874240 |
| identifying schistosoma japonicum excretory/secretory proteins and their interactions with host immune system. | schistosoma japonicum is a major infectious agent of schistosomiasis. it has been reported that large number of proteins excreted and secreted by s. japonicum during its life cycle are important for its infection and survival in definitive hosts. these proteins can be used as ideal candidates for vaccines or drug targets. in this work, we analyzed the protein sequences of s. japonicum and found that compared with other proteins in s. japonicum, excretory/secretory (es) proteins are generally lon ... | 2011 | 21887319 |
| Polymorphism associated with the Schistosoma mansoni tetraspanin-2 gene. | A vaccine against schistosomiasis would contribute significantly to reducing the 3-70 million disability-adjusted life years lost annually to the disease. Towards this end, inoculation with the large extracellular loop (EC-2) of Schistosoma mansoni tetraspanin-2 protein (Sm-TSP-2) has proved effective in reducing worm and egg burdens in S. mansoni-infected mice. The EC-2 loop of Schistosoma japonicum TSP-2, however, has been found to be highly polymorphic, perhaps diminishing the likelihood that ... | 2011 | 21889508 |
| monitoring specific antibody responses against the hydrophilic domain of the 23 kda membrane protein of schistosoma japonicum for early detection of infection in sentinel mice. | schistosomiasis remains an important public health problem throughout tropical and subtropical countries. humans are infected through contact with water contaminated with schistosome cercariae. therefore, issuing early warnings on the risk of infection is an important preventive measure against schistosomiasis. sentinel mice are used to monitor water body infestations, and identifying appropriate antibody responses to schistosome antigens for early detection of infection would help to improve th ... | 2011 | 21906319 |
| oral delivery of the sj23lhd-gst antigen by salmonella typhimurium type iii secretion system protects against schistosoma japonicum infection in mice. | schistosomiasis japonica is a zoonotic parasitic disease and oral vaccine delivery system would be benefit for prevention of this disease. although attenuated salmonella has been used as an antigen expression vector for oral vaccine development, the membrane-bound vacuoles in which bacteria reside hinders the presentation of expressed heterologous antigens to the major histocompatibility complex (mhc) molecules. the present work used an attenuated salmonella typhimurium strain vnp20009 to secret ... | 2011 | 21909450 |
| schistosomiasis research in the dongting lake region and its impact on local and national treatment and control in china. | schistosomiasis is a chronic and debilitating parasitic disease that has often been neglected because it is a disease of poverty, affecting poor rural communities in the developing world. this is not the case in the people's republic of china (prc), where the disease, caused by schistosoma japonicum, has long captured the attention of the chinese authorities who have, over the past 50-60 years, undertaken remarkably successful control programs that have substantially reduced the schistosomiasis ... | 2011 | 21912706 |
| establishing transgenic schistosomes. | 2011 | 21912709 | |
| the sensitivity of artesunate against schistosoma japonicum decreased after 10 years of use in china? | artesuante (as) is a good chemoprophylactic drug for preventing against schistosome infection, but hua et al. recently reported that the sensitivity of as against schistosoma japonicum decreased after 10 years of use in china. there are at least three problems, to our knowledge, making the finding suspicious or inconclusive in that report. in consideration of it as the first report about the emergence of potential artemisinin derivative-resistant s. japonicum to date and the possible severe infl ... | 2011 | 21912960 |
| Migrating Schistosoma japonicum schistosomula induce an innate immune response and wound healing in the murine lung. | The migrating schistosomulum is an important stage of the schistosome lifecycle and represents a key target for elimination of infection by natural and vaccine-induced host immune responses. To gain a better understanding of how schistosomes initiate a primary host immune response we have characterised the host lung response to migrating Schistosoma japonicum schistosomula using a combination of histopathology, microarray analysis and real-time PCR. Our findings indicate that the early pulmonary ... | 2011 | 21917316 |
| the challenge of effective surveillance in moving from low transmission to elimination of schistosomiasis in china. | until recently, intensified efforts in china to suppress the transmission of schistosoma japonicum relied principally on routine praziquantel treatment, extensive use of molluscicides and health education programs. these efforts, now supplemented by a broader range of control measures, have been quite successful in reducing the prevalence and intensity of human infection to very low levels. however, re-emergent transmission has occurred in formerly endemic areas of several provinces, signalling ... | 2011 | 21920366 |
| identification of phage display peptides with affinity for the tegument of schistosoma japonicum schistosomula. | peptides, bound to the tegument of live schistosoma japonicum schistosomula, were differentially screened by phage display in vitro using three rounds of reverse absorption and bio-panning. three m13 phage peptides were isolated and identified by determination of their recovery rate, immunohistochemical localization, immunoblot analysis, and their anti-schistosomal effects in vivo and in vitro. of the three, m13 phage peptide zl4 (mppzl4, ysglqdsslrlr, 1.4kda, pi 8.8) bound to the tegument of me ... | 2011 | 21930161 |