Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| screening of commercial hydrolases for the degradation of ochratoxin a. | ochratoxin a (ota) is a nephrotoxic and carcinogenic mycotoxin. the toxin is a common contaminant of various foods and feeds and poses a serious threat to the health of both humans and animals. a number of commercial hydrolases were screened for the ability to degrade ota to nontoxic compounds. a crude lipase from aspergillus niger (amano a) proved to substantially hydrolyze ota to the nontoxic otalpha and phenylalanine, as confirmed by hplc with fluorescence detection. the enzyme was purified b ... | 2000 | 11087547 |
| a versatile periodate-coupled fluorogenic assay for hydrolytic enzymes we would like to thank prof. r. furstoss and dr. a. archelas at the faculté des sciences de luminy, marseille (france) for providing a sample of aspergillus niger eh and dr. c. weijers at the department of food technology and nutrition sciences, wageningen university (the netherlands) for providing a sample of rhodotorula glutinis eh. this work was supported by the university of bern, the european cost program (action d12), the swiss office fédéral de l'education et de la science, the wander stiftung, the ministero della università e della ricerca scientifica e tecnologica (murst), and consiglio nazionale delle ricerche (cnr) (roma). p.c. gratefully acknowledges merck for generous financial support from the 1999 adp chemistry award. | 2000 | 11093207 | |
| occurrence of itraconazole-tolerant micromycetes in the soil and food products. | unexpected pathogens from the environment represent considerable risk for humans with impaired health. we examined the occurrence of itraconazole tolerant micromycetes in soil and in maize products. five concentrations of itraconazole (2.5-12.5 micrograms/ml) selected according to known treatment schedules for human patients were incorporated into sabouraud agar with chloramphenicol and rose bengal and diluted samples were inoculated onto the agar surface. after 7-d growth at 22 degrees c coloni ... | 1999 | 11097027 |
| copper accumulation and phosphatase activities of aspergillus and rhizopus. | copper accumulation and phosphatase activities of three aspergillus species resistant to copper were compared to three copper-sensitive rhizopus species. high level of acid phosphatases and decreased cu2+-uptake were found with resistant in contrast to sensitive strains. the presence of copper(ii) ions in the medium increased the production of acid phosphatases in the resistant a. niger and decreased their activity in the sensitive r. delemar. copper ions inhibited the activity of a. niger cellu ... | 2000 | 11098820 |
| molecular characterization and influence on fungal development of alp2, a novel serine proteinase from aspergillus fumigatus. | a novel subtilisin-related serine proteinase (alp2) [ec 3.4.21.48] with a broad range of activity between ph 4.5 and 11.0 was released from a cell wall fraction of aspergillus fumigatus by an alkaline ph shift. the enzyme which was not detected in the culture supernatant was partially purified by phenylbutylamine agarose chromatography. the n-terminal sequence revealed that alp2 is the same protein identified as the major allergen of a. fumigatus in patients suffering from extrinsic bronchial as ... | 2000 | 11100830 |
| antimicrobial activity and chemical composition of the bark oil of croton stellulifer, an endemic species from s. tomé e príncipe. | the composition and the antimicrobial activity of the bark oil of croton stellulifer, an endemic and rare species of these islands (s. tomé and príncipe) are reported. analysis was carried out by gc, gc/ms and 13c-nmr. the major constituents were alpha-phellandrene (15.4-18.6%), p-cymene (14.4-17.7%), linalool (12.0-12.6%) and alpha-pinene (8.1-9.1%). kessane, a sesquiterpenoid oxide, not yet reported in the genus croton, was identified by nmr. the essential oil of c. stellulifer was active agai ... | 2000 | 11105572 |
| exchanging the active site between phytases for altering the functional properties of the enzyme. | by using a novel consensus approach, we have previously managed to generate a fully synthetic phytase, consensus phytase-1, that was 15-26 degrees c more thermostable than the parent fungal phytases used in its design (lehmann et al., 2000). we now sought to use the backbone of consensus phytase-1 and to modify its catalytic properties. this was done by replacing a considerable part of the active site (i.e., all the divergent residues) with the corresponding residues of aspergillus niger nrrl 31 ... | 2000 | 11106158 |
| species-specific profiles of mycotoxins produced in cultures and associated with conidia of airborne fungi derived from biowaste. | the potential to produce mycotoxins and non-volatile secondary metabolites was investigated for approximately 250 freshly isolated fungal strains. among the eleven most relevant species, viz. aspergillus flavus, a. fumigatus, a. niger, a. parasiticus, a. versicolor, emericella nidulans, paecilomyces variotii, penicillium brevicompactum, p. clavigerum, p. crustosum, and p. polonicum, a wide range of metabolites partly of toxicological relevance was identified. several unknown metabolites were fou ... | 2000 | 11109562 |
| characterization of aspergillus niger pectate lyase a. | the aspergillus niger plya gene encoding pectate lyase a (ec 4.2.99. 3) was cloned from a chromosomal lambda(embl4) library using the aspergillus nidulans pectate lyase encoding gene [dean, r. a., and timberlake, w. e. (1989) plant cell 1, 275-284] as a probe. the plya gene was overexpressed using a promoter fusion with the a. niger pyruvate kinase promoter. purification of the recombinant pectate lyase a resulted in the identification of two enzyme forms of which one appeared to be n-glycosylat ... | 2000 | 11112543 |
| hepcidin, a urinary antimicrobial peptide synthesized in the liver. | cysteine-rich antimicrobial peptides are abundant in animal and plant tissues involved in host defense. in insects, most are synthesized in the fat body, an organ analogous to the liver of vertebrates. from human urine, we characterized a cysteine-rich peptide with three forms differing by amino-terminal truncation, and we named it hepcidin (hepc) because of its origin in the liver and its antimicrobial properties. two predominant forms, hepc20 and hepc25, contained 20 and 25 amino acid residues ... | 2001 | 11113131 |
| biotransformation of geraniol, nerol and citral by sporulated surface cultures of aspergillus niger and penicillium sp. | the biotransformation of geraniol, nerol and citral by aspergillus niger was studied. a comparison was made between submerged liquid, sporulated surface cultures and spore suspensions. this bioconversion was also carried out with surface cultures of penicillium sp. the main bioconversion products obtained from geraniol and nerol by liquid cultures of a. niger were linalool and alpha-terpineol. linalool, alpha-terpineol and limonene were the main products obtained from nerol and citral by sporula ... | 2000 | 11117886 |
| aspergillus niger i-1472 and pycnoporus cinnabarinus mucl39533, selected for the biotransformation of ferulic acid to vanillin, are also able to produce cell wall polysaccharide-degrading enzymes and feruloyl esterases. | the filamentous fungal strains aspergillus niger i-1472 and pycnoporus cinnabarinus mucl39533, previously selected for the bioconversion of ferulic acid to vanillic acid and vanillin respectively, were grown on sugar beet pulp. a large spectrum of polysaccharide-degrading enzymes was produced by a. niger and very few levels of feruloyl esterases were found. in contrast, p. cinnabarinus culture filtrate contained low amount of polysaccharide-degrading enzymes and no feruloyl esterases. in order t ... | 2001 | 11118600 |
| culture supernatants of patient-derived aspergillus isolates have toxic and lytic activity towards neurons and glial cells. | infection of the central nervous system by the ubiquitous fungi aspergillus spp. is a life-threatening disease. therefore we investigated the mechanism of brain damage by fungal infection. to examine whether secretory factors of aspergillus isolates derived from patients can induce death of different brain cells, culture supernatants of aspergillus fumigatus, aspergillus flavus, aspergillus terreus and aspergillus niger were added to different astrocytes as well as to the neuroblastoma cell line ... | 2000 | 11118912 |
| an anthelmintic compound, nafuredin, shows selective inhibition of complex i in helminth mitochondria. | infections with parasitic helminths are important causes of morbidity and mortality worldwide. new drugs that are parasite specific and minimally toxic to the host are needed to counter these infections effectively. here we report the finding of a previously unidentified compound, nafuredin, from aspergillus niger. nafuredin inhibits nadh-fumarate reductase (complexes i + ii) activity, a unique anaerobic electron transport system in helminth mitochondria, at nm order. it competes for the quinone ... | 2001 | 11120889 |
| studies of in vitro activities of voriconazole and itraconazole against aspergillus hyphae using viability staining. | the minimal fungicidal concentrations (mfcs) of voriconazole and itraconazole for five clinical isolates each of aspergillus terreus, aspergillus fumigatus, aspergillus flavus, and aspergillus niger were determined by a broth macrodilution method. conidial suspensions as inocula were compared to hyphae as inocula since the invasive form of aspergillosis is manifested by the appearance of hyphal structures. in addition, cell viability staining with the dye fun-1 was performed to assess time-depen ... | 2001 | 11120954 |
| on the galactosyl distribution of commercial galactomannans. | a simple method was developed that enabled the enzymatic determination of the galactose distribution in galactomannans. endo-mannanase of aspergillus niger was used to degrade the galactomannan polymers and the degradation products were determined with high-performance anion-exchange chromatography. a whole range of commercial high-to-low substituted galactomannans was analyzed in this way. it was found that differences in the anion-exchange chromatograms reflected dissimilarities in the distrib ... | 2000 | 11128589 |
| contribution of cyanide-insensitive respiratory pathway, catalyzed by the alternative oxidase, to citric acid production in aspergillus niger. | in aspergillus niger, a cyanide (cn)- and antimycin a-insensitive and salicylhydroxamic acid (sham)-sensitive respiratory pathway exists besides the cytochrome pathway and is catalyzed by the alternative oxidase (aox). in this study, a. niger wu-2223l, a citric acid-producing strain, was cultivated in a medium containing 120 g/l of glucose, which is the concentration usually needed for citric acid production, and the effects of 2% (v/v) methanol, an inducer of citric acid, 2 microm antimycin a, ... | 2000 | 11129572 |
| gene cloning and functional characterization by heterologous expression of the fructosyltransferase of aspergillus sydowi iam 2544. | we have purified a fructosyltransferase from conidia of the inulin-producing fungus aspergillus sydowi iam 2544 and obtained peptide sequences from proteolytic fragments of the protein. with degenerated primers, we amplified a pcr fragment that was used to screen a cdna library. the fructosyltransferase gene from aspergillus sydowi (embl accession no. aj289046) is expressed in conidia, while no expression could be detected in mycelia by northern blot analysis of mycelial rna. the gene encodes a ... | 2001 | 11133467 |
| triticum aestivum l. endoxylanase inhibitor (taxi) consists of two inhibitors, taxi i and taxi ii, with different specificities. | the triticum aestivum l. endoxylanase inhibitor (taxi) discovered by debyser and delcour [(1997) eur. pat. filed april 1997, published as wo 98/49278] and debyser, derdelinckx and delcour [(1997) j. am. soc. brew. chem. 55, 153-156] seems to be a mixture of two different endoxylanase inhibitors, called taxi i and taxi ii. by using aspergillus niger as well as bacillus subtilis endoxylanases for assaying inhibition activity, both inhibitors could be purified to homogeneity from wheat (triticum ae ... | 2001 | 11139386 |
| sesquiterpene lactones from centaurea thessala and centaurea attica. antifungal activity. | the aerial parts of centaurea thessala ssp. drakiensis and c. attica ssp. attica afforded, in addition to several known sesquiterpene lactones, two new eudesmanolides, 4-epi-sonchucarpolide and its 8-(3-hydroxy-4-acetoxy-2-methylene-butanoyloxy) derivative and one new eudesmane derivative, named atticin. the in vitro antifungal activity of most compounds was tested against nine fungal species, using the micro-dilution method. all the compounds tested showed great antifungal activity. | 2000 | 11140522 |
| [influence of amaranth on the production of alpha-amylase using aspergillus niger nrrl 3112]. | in this paper the influence of the amaranth seed meal and the aeration conditions on the alpha-amylase production by aspergillus niger nrrl 3112 were studied. the assays of selection of culture medium were carried out in a rotary shaker at 250 rpm and 2.5 cm stroke. the aeration conditions were studied in a mechanically stirred fermentor new brunswick type. a concentration of alpha-amylase of 2750 u.dun/ml was achieved at 120 h with a dry weight of 8.0 g/l, using a base medium with 5.0 g/l amara ... | 2000 | 11149149 |
| glucoamylase: structure/function relationships, and protein engineering. | glucoamylases are inverting exo-acting starch hydrolases releasing beta-glucose from the non-reducing ends of starch and related substrates. the majority of glucoamylases are multidomain enzymes consisting of a catalytic domain connected to a starch-binding domain by an o-glycosylated linker region. three-dimensional structures have been determined of free and inhibitor complexed glucoamylases from aspergillus awamori var. x100, aspergillus niger, and saccharomycopsis fibuligera. the catalytic d ... | 2000 | 11150611 |
| phase transfer and biocatalyst behaviour during biotransformation of beta-ionone in a two-phase liquid system by immobilised aspergillus niger. | the biotransformation of beta-ionone by aspergillus niger ifo 8541 entrapped in ca-alginate beads was investigated in a two-phase liquid system, due to the low aqueous solubility of the precursor. modelling of phase transfer processes of the substrate demonstrated that the solute was transferred from the organic droplets to the gas, giving a loss by stripping, and then from the gas to the aqueous solution where a chemical degradation occurred. the biological reaction took place after direct prec ... | 2001 | 11150793 |
| combined molecular and biochemical approach identifies aspergillus japonicus and aspergillus aculeatus as two species. | we examined nine aspergillus japonicus isolates and 10 aspergillus aculeatus isolates by using molecular and biochemical markers, including dna sequences of the its1-5.8s rrna gene-its2 region, restriction fragment length polymorphisms (rflp), and secondary-metabolite profiles. the dna sequence of the internal transcribed spacers (its1 and its2) and the 5.8s rrna gene could not be used to distinguish between a. japonicus and a. aculeatus but did show that these two taxa are more closely related ... | 2001 | 11157212 |
| combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in fusarium venenatum. | fusarium venenatum jers 325, a strain which produces recombinant glucoamylase under control of a growth rate independent promoter was transformed with a plasmid carrying the aspergillus niger glucoamylase gene under control of its own growth rate correlated promoter. some disruption of the original recombinant genes occurred and at ph 5.8 the double transformant did not produce as much glucoamylase as jers 325 in batch culture. however, the double transformant still produced as much glucoamylase ... | 2001 | 11164313 |
| structure-based chimeric enzymes as an alternative to directed enzyme evolution: phytase as a test case. | thermostability is a key feature for commercially attractive variants of the fungal enzyme phytase. in an initial set of experiments, we restored ionic interactions and hydrogen bonds on the surface of aspergillus terreus phytase, which are present in the homologous but more thermostable enzyme from a. niger. since these mutations turned out to be neutral, we replaced-in the same region and based on the crystal structure of a. niger phytase-entire secondary structure elements. the replacement of ... | 2001 | 11164958 |
| diagnosis at first glance: hairy black colonies in resected prosthetic aortic valve. | 2000 | 11168066 | |
| effects of metal ions on simultaneous production of glucose oxidase and catalase by aspergillus niger. | the effects of various metal ions on the simultaneous production of glucose oxidase and catalase by aspergillus niger were investigated. calcium carbonate induced synthesis of both enzymes. the induction of calcium carbonate was accompanied by a metabolic shift from the glycolytic pathway (emp, embden-meyerhof-parnas) to direct oxidation of glucose by glucose oxidase. the time course of the biosynthesis of both enzymes is reported. the logistic model was in good agreement with the experimental g ... | 2001 | 11169035 |
| the influence of ph upon the kinetic parameters of the enzymatic hydrolysis of cellobiose with novozym 188. | we have studied experimentally within the ph range of 3.65-5.5 at 50 degrees c the hydrolysis of cellobiose with novozym 188, a commercial product with high beta-1,4-glucosidase activity derived from aspergillus niger. we used wide variations in the conversion to be able to apply the integral method and thus determine that there is substrate and mixed product inhibition. whether the ses triple compound contributes to the formation of glucose does not influence the fitting of the experimental res ... | 2001 | 11170487 |
| the effect of organic nitrogen sources on recombinant glucoamylase production by aspergillus niger in chemostat culture. | aspergillus niger b1, a recombinant strain carrying 20 extra copies of the native glucoamylase gene, was grown in glucose-limited chemostat cultures supplemented with various organic nitrogen sources (dilution rate 0.12 +/- 0.01 h(-1), ph 5.4). in cultures supplemented with l-alanine, l-methionine, casamino acids, or peptone, specific glucoamylase (gam) production rapidly decreased to less than 20% of the initial level. reducing the ph of the culture to 4.0 resulted in stable gam production for ... | 2000 | 11170742 |
| phytochemical and antimicrobial properties of constituents of "ogwu odenigbo", a popular nigerian herbal medicine for typhoid fever. | the ethylacetate-insoluble fraction of the methanol extract of "ogwu odenigbo" a popular nigerian traditional herbal medicine for typhoid fever prepared from the stem bark of cleistropholis patens benth, (annonaceae), was separated into 13 semi-characterized constituents by preparatory tlc. the in vitro antimicrobial activities of the 13 fractions were quantitatively and/or qualitatively assessed by the agar well diffusion method using staphylococcus aureus, bacillus subtilis, escherichia coli, ... | 2001 | 11180528 |
| bioactivity studies of extracts from tridax procumbens. | an updated review on the biological activity of tridax procumbens is presented. a detailed biological screening comprised of gram-positive and gram-negative bacteria, yeasts and fungi using crude extracts of this plant was undertaken. the n-hexane extract of the flowers showed activity against escherichia coli. the same extract of the whole aerial parts was active against mycobacterium smegmatis, escherichia coli, salmonella group c and salmonella paratyphi. the ethyl-acetate extract of the flow ... | 2000 | 11185735 |
| [studies on the regulation of glucoamylase gene (glaa) expression in a. niger i. the overall analysis and comparison of the expression of glucoamylase in the overproducing strain a. niger t21 and its original strain a. niger 3.795]. | expression of the a. niger t21 and 3.795 glaa gene was investigated with respect to the growth of mycelia, producing of glucoamylase, copy number of glaa gene as well as glucoamylase mrna level and its stability. both a. niger t21 and 3.795 produced substantial glucoamylase at the stable phase when their biomass were the same. after incubating for 72 hours, while the biomass remained the same, the glucoamylase produced by t21 was 10-17 times higher than that by 3.795. northern analysis showed th ... | 1997 | 11189359 |
| [purification and properties of inulinase from aspergillus niger]. | the main component of inulinase was purified from fermentation broth of aspergillus niger 319 to homogeneity by using ammonium sulfate fraction, ion-exchange chromatography on deae-cellulose column and sephadex g-100 gel filtration. the specific activity was as 67 folds at the fermentation broth, and the yield was 25.5%. the inulinase, containing 13.92% of carbohydrate, was a monomer protein with a molecular weight of 28,000 dalton; and its isoelectric point was ph 5.4. the optimal ph and temper ... | 1997 | 11189361 |
| [study on gallic acid preparation by using immobilized tannase from aspergillus niger]. | immobilized tannase which had higher ability of transforming tannin from chinese gallotannin into ga was prepared by embedding tannase with calcium alginate carrier. the immobilization conditions and some properties of the immobilized tannase were studied. the results showed that: the optimal immobilized parametres were 90 mg sodium alginate, 546 u(182 u/ml) tannase and 1%-2% cacl2. the optimum temperature and ph value of immobilized tannase were 45 degrees c and 6.5, respectively. it was stable ... | 2000 | 11191769 |
| molecular cloning and sequence analysis of an endoinulinase gene from penicillium sp. strain tn-88. | a genomic dna segment and cdnas encoding an extracellular endoinulinase of penicillium sp. strain tn-88 were cloned and sequenced. southern blot analysis indicated that the endoinulinase gene (inuc) was present as a single copy in the genome. an open reading frame, consisting of 1,545 bp, was not interrupted by introns, and it encoded a 25 amino acid signal peptide and a 490 amino acid mature protein. the mature protein contained three cys residues and ten potential n-linked glycosylation sites. ... | 2000 | 11193399 |
| microbiological survey of retail herbs and spices from mexican markets. | in the present study, 304 samples of herbs and spices (garlic powder, cumin seeds, black pepper, oregano, and bay leaves) widely used in mexico were analyzed for the presence of bacillus cereus, salmonella typhi, shigella dysenteriae, escherichia coli, total and fecal coliforms, total mesophilic aerobic organisms, and fungi. samples were nonpackaged or packaged in polyethylene bags or glass containers. high levels (10(5) to 10(7) cfu/g) of mesophilic aerobic microorganisms were found in most of ... | 2001 | 11198448 |
| identification of medically important aspergillus species by single strand conformational polymorphism (sscp) of the pcr-amplified intergenic spacer region. | the amplified 5.8s rna coding dna with the neighbouring internal transcribed spacers its i and its ii (its i--5.8s rdna--its ii) of 27 culture collection strains of aspergillus fumigatus, aspergillus flavus, aspergillus nidulans, aspergillus niger, and aspergillus terreus were investigated by single strand conformational polymorphism (sscp) analysis. all strains showed a polymerase gel electrophoresis (pcr) product of 0.6 kb. separation of dna single strands of the pcr product in an acrylamide-b ... | 2000 | 11204354 |
| aspergillus niger absorbs copper and zinc from swine wastewater. | wastewater from swine confined-housing operations contains elevated levels of copper and zinc due to their abundance in feed. these metals may accumulate to phytotoxic levels in some agricultural soils of north carolina due to land application of treated swine effluent. we evaluated fungi for their ability to remove these metals from wastewater and found aspergillus niger best suited for this purpose. a. niger was able to grow on plates amended with copper at a level five times that inhibitory t ... | 2001 | 11211074 |
| needle-type glucose microbiosensor based on glucose oxidase immobilised in an overoxidised polypyrrole film (an in-vitro study). | a fast response, needle-type glucose microbiosensor has been fabricated by a one-step electrochemical immobilisation of glucose oxidase in a polypyrrole film. the sensor shows a remarkable rejection of electroactive interferences, especially paracetamol. the maximum bias observed in the worst situation never exceeded the value of 6%. the fabrication procedure delivered very reproducible devices and the sensitivity of a newly prepared biosensor was typically 650 na mm(-1) cm(-2). the kinetic para ... | 2000 | 11220601 |
| new antifungal constituents from terminalia alata. | the roots of terminalia alata yielded three new glycosides: 3,3'-di-o-methylellagic acid 4-o-beta-d-glucopyranosyl-(1-->4)-beta-d-glucopyranosyl-(1-->2)-alpha-l-arabinopyranoside (1), 5,7,2'-tri-o-methylflavanone 4'-o-alpha-l-rhamnopyranosyl-(1-->4)-beta-d-glucopyranoside (2), and 2alpha,3beta, 19beta,23-tetrahydroxyolean-12-en-28-oic acid 3-o-beta-d-galactopyranosyl-(1-->3)-beta-d-glucopyranoside-28-o-beta-d-glucopyranoside (3). compounds 2 and 3 displayed antifungal activity. | 2001 | 11223218 |
| crystallization and preliminary x-ray analysis of a novel pectolytic enzyme, polymethoxygalacturonase sx1 from trichosporon penicillatum. | a novel pectolytic enzyme, polymethoxygalacturonase sx1 from trichosporon penicillatum, with a molecular weight of 36 kda was crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 1000 as a precipitant. the crystals belonged to the monoclinic space group c2, with unit-cell parameters a = 165.6, b = 61.0, c = 48.7 a, beta = 93.1 degrees. the calculated v(m) based on one molecule per asymmetric unit was 3.40 a(3) da(-1). a native data set was collected to 2.08 a resolu ... | 2001 | 11223531 |
| incidence of allergenically significant fungal aerosol in a rural bakery of west bengal, india. | the frequency of fungal spores in the air of three different sections of a rural bakery was analyzed using a burkard personal slide sampler and andersen two stage viable sampler. in average concentration of spores (no./m3) was 228-26770/m3 and concentration of viable colony forming units (cfu/m3) was 65-2061 cfu/m3. dominant fungus species both culturable and nonculturable, were species of aspergillus and penicillium, cladosporium sp., aspergillus niger, aspergillus flavus, aspergillus fumigatus ... | 2000 | 11227852 |
| mycoflora and mycotoxins in brazilian black pepper, white pepper and brazil nuts. | a wide range of field and storage fungi were isolated from black pepper, white pepper and brazil nut kernels from amazonia. a total of 42 species were isolated from both peppers. aspergillus flavus and a. niger were isolated more frequently from black than from white pepper. other potential mycotoxigenic species isolated included: a. ochraceus, a. tamarii, a. versicolor, emericella nidulans and chaetomium globosum, penicillium brevicompactum, p. citrinum, p. islandicum and p. glabrum. species is ... | 2000 | 11229375 |
| fungal metabolism of toluene: monitoring of fluorinated analogs by (19)f nuclear magnetic resonance spectroscopy. | we used isomeric fluorotoluenes as model substrates to study the catabolism of toluene by five deuteromycete fungi and one ascomycete fungus capable of growth on toluene as the sole carbon and energy source, as well as by two fungi (cunninghamella echinulata and aspergillus niger) that cometabolize toluene. whole cells were incubated with 2-, 3-, and 4-fluorotoluene, and metabolites were characterized by (19)f nuclear magnetic resonance. oxidation of fluorotoluene by c. echinulata was initiated ... | 2001 | 11229888 |
| fungal metabolites from aspergillus niger an27 related to plant growth promotion. | two metabolites have been isolated from aspergillus niger an27, a biocontrol agent, and identified as 2-carboxymethyl 3-n-hexyl maleic acid (compound 1) and 2-methylene-3-hexylbutanedioic acid (compound 2). their biological activities related to crop growth promotion have been assayed. both the compounds increased germination and improved crop vigour. compound 1 was more effective for increase in germination and shoot length, whereas compound 2 had relatively greater role in increasing the root ... | 2000 | 11233092 |
| ultrasonic depolymerization of the chitin-glucan complex from aspergillus niger and antimutagenic activity of its product. | sonication was effective for the depolymerization of carboxymethylated chitin-glucan complex (cm-cg) isolated from the cell wall of aspergillus niger. after 10 min of sonication and subsequent gel filtration, two samples (cm-cg(i) and cm cg(ii)) with significantly distinct molecular weights (660 kda and 19 kda, respectively) and different nitrogen contents (3.02 and 1.69%) were obtained. cm-cg(ii) with lower mw was also effective against cyclophosphamide mutagenicity by oral administration in mi ... | 1999 | 11233930 |
| [the use of micromycetes for cleaning parts of aircraft]. | the mycelial fungi penicillium funiculosum, p. citrinum, p. expansum, p. chrysogenum, aspergillus ochraceus, a. alliaceus, a. luchaensis, a. flavus, and a. niger were isolated from enrichment cultures. these fungi actively destruct carbon deposits formed during exploitation of aircraft. a biotechnological method for removing fouling from parts of aircraft engines (pae) was developed. this method is less laborious, more rapid and ecologically clean than contemporary chemical methods. scanning mic ... | 2001 | 11234409 |
| effect of phospholipids transesterified enzymatically with polyunsaturated fatty acids on gelatinization and retrogradation of starch. | the effects of phospholipids (pls) transesterified with polyunsaturated fatty acids (pufas) with lipase (aspergillus niger) on gelatinization and retrogradation of starch during storage were studied by differential scanning calorimetry (dsc). the resulting transesterified pls were rich in pufas and linoleic acid, while the total percentage of pufas incorporated was 20.2%. the addition of pls or pls enzymatically transesterified with pufas (pufa-pls) to the starch sample decreased the gelatinizat ... | 2000 | 11234919 |
| a novel chitosan derivative to immobilize alpha-l-rhamnopyranosidase from aspergillus niger for application in beverage technologies. | alpha-l-rhamnopyranosidase (rha, ec 3.2.1.40) is an enzyme of considerable importance to food technology in increasing the aroma of wines, musts, fruit juices and other beverages. the aim of this research is the immobilization of the rha contained in a commercial preparation already used in the winemaking industry and purified in the manner described in a previous study [1]. the immobilization supports tested were chitin, chitosan and derivatized chitosan, diethylaminoethyl chitosan (de-chitosan ... | 2001 | 11240202 |
| the antioxidant system beta-d(+) glucose-glucose oxidase-catalase: tests for pyrogenicity and antigenicity. | beta-d(+) glucose-glucose oxidase (e.c.# 1.1.3.4)-catalase (e.c.# 1.11.1.6) (go-cat) is being investigated as a new antioxidant system for use in pharmaceutical solutions. this study reports the results of tests for pyrogenicity and antigenicity of go-cat derived from aspergillus niger when used parenterally in autoclaved preparations. | 2001 | 11247273 |
| [screening of alpha-n-acetylgalactosaminidase producers among microorganisms of different taxonomic groups]. | screening of producers of alpha-n-acetylgalactosaminidase among 854 strains of micromycetes, 171 yeast and 357 bacterial cultures has been carried out. a capacity to synthesize the enzyme was revealed in 11% of cultures. representatives of aspergillus genus (activity--0.11-0.142 un./ml) were most active in producing the enzyme. it has been established that glucosidases spectrum in the culture liquid of 18 most active strains was characterized by complete homogeneity and by the presence of a rath ... | 2000 | 11247342 |
| evolution of a recombinant (gucoamylase-producing) strain of fusarium venenatum a3/5 in chemostat culture. | fusarium venenatum jers 325 is a transformant of strain a3/5 which produces aspergillus niger glucoamylase (gam) under the control of a fusarium oxysporum trypsin-like protease promoter. the evolution of jers 325 was studied in glucose-limited chemostat cultures grown on nano3 or (nh4)2so4 as the nitrogen source. thirteen mutants which were more highly branched and four mutants which were more sparsely branched than the parental strain were isolated from the nano3 chemostat. the highly branched ... | 2001 | 11255162 |
| gpda-promoter-controlled production of glucose oxidase by recombinant aspergillus niger using nonglucose carbon sources. | the gpda-promoter-controlled exocellular production of glucose oxidase (god) by recombinant aspergillus niger nrrl-3 (god3-18) during growth on glucose and nonglucose carbon sources was investigated. screening of various carbon substrates in shake-flask cultures revealed that exocellular god activities were not only obtained on glucose but also during growth on mannose, fructose, and xylose. the performance of a. niger nrrl-3 (god3-18) using glucose, fructose, or xylose as carbon substrate was c ... | 2001 | 11257807 |
| a new bio-active flavonol glycoside from the stems of butea superba roxb. | a new bio-active flavonol glycoside was isolated from the stems of butea superba roxb, and its structure was determined by spectral analysis and chemical degradations as 3,5,7,3',4'-pentahydroxy-8-methoxy-flavonol-3-o-beta-d-xylopyranosyl(1-->2) -alpha-l-rhamnopyranoside. the compound 1 showed antimicrobial activity against plant pathogenic fungi trich viride, asprgillus fumigatus, a. niger, a. terreus, penicillium expansum, helmitnhosporium oryzae, botxitis cinerea, rhizopus oligosporus, r. chi ... | 1998 | 11261215 |
| cloning and expression of glucose 3-dehydrogenase from halomonas sp. alpha-15 in escherichia coli. | the gene encoding glucose 3-dehydrogenase (g3dh) from halomonas sp. alpha-15 was cloned and expressed in escherichia coli. an open reading frame of 1686 nucleotides was shown to encode g3dh. the flavine adenine dinucleotide binding motif was found in the n-terminal region of g3dh. the deduced primary structure of g3dh showed about 30% identity to sorbitol dehydrogenase from gluconobacter oxydans and 2-keto-d-gluconate dehydrogenases from erwinia herbicola and pantoea citrea. the folding predicti ... | 2001 | 11263965 |
| new cyclic depsipeptide antibiotics, clavariopsins a and b, produced by an aquatic hyphomycetes, clavariopsis aquatica. 1. taxonomy, fermentation, isolation, and biological properties. | clavariopsins were isolated from the fermentation broth of clavariopsis aquatica aj 117363. clavariopsins are cyclic depsipeptide antibiotics with the molecular weight of 1,153 and 1,139. clavariopsins showed in vitro antifungal activity against not only aspergillus fumigatus but also, although to a lesser extent, a. niger and candida albicans. | 2001 | 11269710 |
| effect of ultrasonic treatment on the molecular weight of carboxymethylated chitin-glucan complex from aspergillus niger. | different factors affecting the efficiency of the ultrasonic depolymerization of the high-molecular-weight carboxymethylated chitin-glucan prepared from the fungal mycelium of aspergillus niger have been investigated. the influence of the following parameters was examined: concentration of the chitin-glucan complex, duration of ultrasonic irradiation, reaction temperature and volume of the ultrasonicated solution. the optimized conditions for the efficient ultrasonic depolymerization include: po ... | 1999 | 11269957 |
| synergistic interaction of terbinafine with triazoles or amphotericin b against aspergillus species. | the in vitro activity of terbinafine alone and in combination with other antifungal agents was tested against isolates of aspergillus fumigatus, a. flavus and a. niger. testing was performed in a modified national committee for clinical laboratory standards (nccls) macrodilution broth assay, and interactions were examined using a checkerboard design. terbinafine was highly active against aspergillus isolates (minimum inhibitory concentration [mic] 0.01 to 2 microg ml(-1)) with a primary fungicid ... | 2001 | 11270414 |
| regulation of chitinase 33 (chit33) gene expression in trichoderma harzianum. | we investigated the regulation of chit33 expression in trichoderma harzianum cect 2413. this gene encodes the chit33 endochitinase, which is a major component of the fungus' chitinolytic enzyme system and is important for biocontrol. to this end, both northern analysis and reporter gene fusions of a 1.4-kb fragment of the 5'-upstream sequences of chit33 to the aspergillus niger goxa gene (encoding glucose oxidase) and the aquorea victoria green fluorescent protein were used. northern analysis an ... | 2001 | 11270576 |
| formation of myo-inositol phosphates by aspergillus niger 3-phytase. | kinetics of phytate hydrolysis by aspergillus niger phytase and correlation between the amount of released phosphate and creation of lower myo-inositol phosphates were investigated. phytase was able to hydrolyze myo-inositol hexakis-, pentakis-, tetrakis-, and trisphosphates. finally, about 56% of total phosphate were released and myo-inositol bisphosphate was detected as the end-product. | 2000 | 11271819 |
| production, purification and properties of microbial phytases. | phytases (myo-inositol hexakisphosphate phosphohydrolase, ec 3.1.3.8) catalyse the release of phosphate from phytate (mycoinositol hexakiphosphate). several cereal grains, legumes and oilseeds, etc., store phosphorus as phytate. environmental pollution due to the high-phosphate manure, resulting in the accumulation of p at various locations has raised serious concerns. phytases appear of significant value in effectively controlling p pollution. they can be produced from a host of sources includi ... | 2001 | 11272007 |
| antifungal activity of bicyclic heterocyclic-1,2-diazole. | a novel series of heterocyclic-1,2 diazole namely n1-iso nicotinoyl-5,5'-dimethyl cyclohexane-4-(sulpha/substituted phenylazo)-1,2-diazoles have been synthesized. the compounds were screened for the anti-fungal properties against building fungi. the fungal species used for this purpose were aspergillus niger and pencillium frequentans. it was found that out of a series of 25 compounds, fourteen have shown significant fungicidal properties against both the above species. minimum inhibition concen ... | 2000 | 11272421 |
| sequence analysis and overexpression of a pectin lyase gene (pel1) from aspergillus oryzae kbn616. | a gene (pel1) encoding pectin lyase (pel1) was isolated from a shoyu koji mold, aspergillus oryzae kbn616, and characterized. the structural gene comprised 1,196 bp with a single intron. the orf encoded 381 amino acids with a signal peptide of 20 amino acids. the deduced amino acid sequence showed high similarity to those of aspergillus niger pectin lyases and glomerella cingulata pnla. the pel1 gene was successfully overexpressed under the promoter of the a. oryzae tef1 gene. the molecular mass ... | 2001 | 11272833 |
| synthesis and in-vitro antimicrobial activity of new 1,2,4-triazoles. | we have described the synthesis of new 1,2,4-triazoles and have evaluated their antimicrobial profile. antitubercular activity was determined in triplicate using the lowenstein-jensen medium. a loopful of mycobacterium tuberculosis suspension was inoculated on the surface of each lowenstein-jensen media containing the test compounds (100, 10 or 1 microg ml(-1)). to evaluate in-vitro antibacterial activity, compounds (50, 5 or 0.5 microg) were evaluated against b. subtilis, escherichia coli, pseu ... | 2001 | 11273026 |
| a refined solution structure of hen lysozyme determined using residual dipolar coupling data. | a high resolution nmr structure of hen lysozyme has been determined using 209 residual 1h-15n dipolar coupling restraints from measurements made in two different dilute liquid crystalline phases (bicelles) in conjunction with a data set of 1632 noe distance restraints, 110 torsion angle restraints, and 60 hydrogen bond restraints. the ensemble of 50 low-energy calculated structures has an average backbone rmsd of 0.50+/-0.13a to the mean structure and of 1.49+/-0.10a to the crystal structure of ... | 2001 | 11274458 |
| a study of the protein secretory pathway of aspergillus niger using a glucoamylase-gfp fusion protein. | the effect of various treatments that block protein secretion was visualized in aspergillus niger using a strain expressing a glucoamylase-gfp fusion protein. cold shock caused the retention of the fusion protein in a reticulate network (er) with brighter nodes that may represent golgi bodies. treatment of germlings with brefeldin a (bfa) also initially caused accumulation within the er but prolonged exposure led to the formation and targeting of the fusion protein to vacuoles from the er. disru ... | 2001 | 11277626 |
| expression of the caldariomyces fumago chloroperoxidase in aspergillus niger and characterization of the recombinant enzyme. | the caldariomyces fumago chloroperoxidase was successfully expressed in aspergillus niger. the recombinant enzyme was produced in the culture medium as an active protein and could be purified by a three-step purification procedure. the catalytic behavior of recombinant chloroperoxidase (rcpo) was studied and compared with that of native cpo. the specific chlorination activity (47 units/nmol) of rcpo and its ph optimum (ph 2.75) were very similar to those of native cpo. rcpo catalyzes the oxidati ... | 2001 | 11278701 |
| deoxysugars via microbial reduction of 5-acyl-isoxazolines: application to the synthesis of 3-deoxy-d-fructose and derivatives. | 5-acylisoxazolines 3a-d were obtained by 1,3-dipolar cycloaddition from acetoxymethyl vinyl ketone and nitro precursors. compounds 3a-d were biotransformed by aspergillus niger into a 1:1 mixture of stereomers of 5-dihydroxyethyl isoxazolines (+)-4a-d (anti) and (-)-5a-d (syn). both stereomers were obtained in good yields and with high optical purities. carbonyl reduction by aspergillus niger produces alcohols of r-configuration thus giving an access to d-sugar analogues: compound (+)-4d was con ... | 2001 | 11281769 |
| synthetic applications of epoxide hydrolases. | there have been several recent advances in the area of biocatalysed hydrolytic kinetic resolution of epoxides using 'newly discovered' enzymes (i.e. epoxide hydrolases). these biocatalysts, two of which will become commercially available in the near future, appear to be highly promising tools for fine organic synthesis, as they enable the preparation of various epoxides and/or their corresponding diols in enantiopure form. | 2001 | 11282336 |
| osteomyelitis of the temporomandibular joint. | osteomyelitis of the temporomandibular joint is very uncommon, and osteomyelitis as a result of aspergillus niger infection has not previously been reported. a case report of skull base and condylar osteomyelitis is presented. previously reported cases of temporomandibular joint osteomyelitis are reviewed, and management is discussed. because of the significant morbidity possible with infections in this region, otolaryngologists should be familiar with the anatomy, diagnostic modalities, and the ... | 2001 | 11283831 |
| removal of herbicides from liquid media by fungi isolated from a contaminated soil. | fungi were isolated from soil samples corresponding to pesticide-contaminated soil (cs) and noncontaminated soil (ncs) in the annaba vicinity (algeria) and identified. the number of isolates obtained from cs and ncs were 263 and 288, respectively. the most frequent species (aspergillus fumigatus, a. niger, a. terreus, absidia corymbifera, and rhizopus microsporus var microsporus) were not sensitive to the pesticides. the growth of the genus trichoderma was inhibited by the pesticides, while gene ... | 2001 | 11285902 |
| [results of the first dermatomycoses quality control study in germany]. | for the first time in the history of german mycology, a multicenter study for identification of dermatophytes, yeasts and molds was conducted under the auspices of the german speaking mycological society and the german dermatological society. a total of 1,008 dermatological offices and dermatology hospitals as well as 106 institutes of microbiology participated in this voluntary quality control trial. they were requested to identify four pathogens: trichophyton tonsurans, trichophyton mentagroph ... | 2000 | 11291580 |
| aceii, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of trichoderma reesei. | a novel yeast-based method to isolate transcriptional activators was applied to clone regulators binding to the cellulase promoter cbh1 of the filamentous fungus trichoderma reesei (hypocrea jecorina). this led to the isolation of the cellulase activator ace2 encoding for a protein belonging to the class of zinc binuclear cluster proteins found exclusively in fungi. the dna-binding domain of aceii was expressed as a glutathione s-transferase fusion protein in escherichia coli, and aceii was show ... | 2001 | 11304525 |
| extracellular secretion of aspergillus phytase from arabidopsis roots enables plants to obtain phosphorus from phytate. | phosphorus (p) deficiency in soil is a major constraint for agricultural production worldwide. despite this, most soils contain significant amounts of total soil p that occurs in inorganic and organic fractions and accumulates with phosphorus fertilization. a major component of soil organic phosphorus occurs as phytate. we show that when grown in agar under sterile conditions, arabidopsis thaliana plants are able to obtain phosphorus from a range of organic phosphorus substrates that would be ex ... | 2001 | 11319031 |
| citric and gluconic acid production from fig by aspergillus niger using solid-state fermentation. | the production of citric and gluconic acids from fig by aspergillus niger atcc 10577 in solid-state fermentation was investigated. the maximal citric and gluconic acids concentration (64 and 490 g/kg dry figs, respectively), citric acid yield (8%), and gluconic acid yield (63%) were obtained at a moisture level of 75%, initial ph 7.0, temperature 30 degrees c, and fermentation time in 15 days. however, the highest biomass dry weight (40 g/kg wet substrate) and sugar utilization (90%) were obtain ... | 2000 | 11320416 |
| bioactive constituents of the roots of licania intrapetiolaris. | fractionation of a methanol extract of the roots of licania intrapetiolaris, as directed by activity against the kb assay, has led to the isolation of two novel clerodane diterpenoids, intrapetacins a (1) and b (2), and the known triterpenoid cucurbitacin b (3). the structures of 1 and 2 were deduced from one- and two-dimensional nmr experiments, including relative stereochemical assignments based on noesy correlations and cosy coupling constants. compound 3 was the most potent against the kb as ... | 2001 | 11325234 |
| monovalent cation-induced conformational change in glucose oxidase leading to stabilization of the enzyme. | glucose oxidase (god) from aspergillus niger is an acidic dimeric enzyme having a high degree of localization of negative charges on the enzyme surface and dimer interface. we have studied the effect of monovalent cations on the structure and stability of god using various optical spectroscopic techniques, limited proteolysis, size exclusion chromatography, differential scanning calorimetry, and enzymic activity measurements. the monovalent cations were found to influence the enzymic activity an ... | 2001 | 11329261 |
| synthesis, structural characterization and antimicrobial activities of 4- and 6-coordinate nickel(ii) complexes with three thiosemicarbazones and semicarbazone ligands. | to investigate the relationship between antimicrobial activities and the molecular structures of nickel(ii) complexes with thiosemicarbazone and semicarbazone ligands, nickel(ii) complexes with ligands hmtsc, hatsc, hasc and h2dmtsc, were prepared and characterized by elemental analysis, ft-ir, 1h and 13c nmr spectroscopies, magnetic susceptibility measurements, uv-vis absorption spectra, tg/dta and single-crystal x-ray analysis. their antimicrobial activities were evaluated by the mic against f ... | 2001 | 11330482 |
| spectrometric evidence for the flavin-1-phenylcyclopropylamine inactivator adduct with monoamine oxidase n. | 1-phenylcyclopropylamine (1-pcpa) is shown to be an inactivator of the fungal flavoenzyme monoamine oxidase (mao) n. inactivation results in an increase in absorbance at 410 nm and is accompanied by the concomitant loss of the flavin absorption band at 458 nm. the spectral properties of the covalent adduct formed between the flavin cofactor of mao n and 1-pcpa are similar to those reported for the irreversible inactivation product formed with 1-pcpa and mammalian mitochondrial monoamine oxidase ... | 2001 | 11331009 |
| production of glucose oxidase using aspergillus niger and corn steep liquor. | glucose oxidase production was optimized using an isolated strain of aspergillus niger and an economical nutrient source, corn steep liquor (csl). the culture produced 580 +/- 30 units/ml of the enzyme using 70 g/l sucrose as the carbon source. using csl as the sole nutrient source enzyme synthesis was increased to 640 +/- 36 units/ml. none of the nitrogen sources (nitrates of calcium, sodium, ammonium, potassium and yeast extract, malt extract, and peptone) was beneficial to the enzyme synthesi ... | 2001 | 11333029 |
| bioadsorption of lead(ii) from aqueous solution by fungal biomass of aspergillus niger. | the removal of lead by waste fungal biomass of aspergillus niger, originated from citric acid fermentation industry, was investigated. the experimental results indicated that the bioadsorption achieved equilibrium within 4 h. the kinetic analysis of lead adsorption onto the fungal biomass revealed that the bioadsorption process followed the first-order reaction kinetics. the adsorption isotherm can be simulated by freundlich model, which gave a correlation coefficient equal to 0.93. the lead-loa ... | 2001 | 11334669 |
| hydrolysis of rice hull by crosslinked aspergillus niger cellulase. | a. niger cellulase was crosslinked by glutaraldehyde to obtain a heat-stable enzyme preparation for rice hull cellulose hydrolysis. under optimized crosslinking conditions of 0.12 m glutaraldehyde, ph 7.0, temperature 40 degrees c and at 45 min of crosslinking, a preparation having 15% more activity than free enzyme was obtained which also had considerable improvement in heat stability at 65 degrees c and 70 degrees c. whereas the free enzyme lost 80% of its activity in 4 h at 65 degrees c, the ... | 2001 | 11341689 |
| an extracellular beta-galactofuranosidase from aspergillus niger and its use as a tool for glycoconjugate analysis. | aspergillus niger produces an extracellular beta-galactofuranosidase, which can specifically hydrolyse beta-d-galactofuranose (galf) from glycoconjugates. the production of this enzyme can be induced by the addition of a galf-containing a. niger mycelial wall extract. however, on other carbon sources accumulation occurred only during the starvation conditions of the late stationary phase. extracellular glucoamylases from this stage of cultivation possessed significantly lower levels of galf than ... | 2001 | 11342249 |
| airborne fungal ecological niche determination as one of the possibilities for indirect mycotoxin risk assessment in indoor air. | based on the microbiological analysis of air samples from occupied spaces, some possibilities for indirect risk assessment of mycotoxin-related health problems are proposed. airborne fungi could be classified on the basis of the relationship between the two environmental factors and their combinations, i.e., temperature and water requirements (water activity aw). one type involves three different groups of molds, selected on the basis of the quantitative and qualitative information about the abi ... | 2001 | 11345539 |
| effect of cadmium and copper on the production of citric acid by aspergillus niger. | copper and cadmium inhibited the growth as well as citric acid production (depending on the heavy metal concentrations) by citric-acid-producing aspergillus niger. activity of citrate synthase was connected with citrate synthesis in the absence as well as in the presence of heavy metals. the activity of aconitase, and both nad- and nadp-isocitrate dehydrogenases was strongly inhibited by copper. the contents of dna and proteins in the cells decreased but the contents of lipids and polysaccharide ... | 2000 | 11347255 |
| mycotoxin-producing potential of fungi associated with qat (catha edulis) leaves in yemen. | forty-four fungal species belonging to 20 genera were isolated from 30 samples of qat leaves. the most frequent genera were aspergillus, alternaria, penicillium, and cladosporium followed by fusarium, drechslera, chaetomium, and mucor. the most prevalent species in above genera were aspergillus niger, a. flavus, a fumigatus, alternaria alternata, penicillium chrysogenum, p. citrinum, cladosporium cladosporioides, and fusarium verticillioides. from these fungi, 17 species (39%) related to 7 gener ... | 2000 | 11347273 |
| mycoflora in commercial pet foods. | this article reports on the identification of mycoflora of 21 dry pet foods (12 belonging to dogs and 9 to cats) that corresponded to 8 commercial brands made in argentina and imported. the isolation frequency and relative density of the prevalent fungal genera are compared too. ten genera and fungi classified as mycelia sterilia were identified. the predominant genera were aspergillus (62%), rhizopus (48%), and mucor (38%). the most prevalent among aspergillus was aspergillus flavus followed by ... | 2001 | 11348013 |
| estimation of triterpenoids from heliotropium marifolium koen. ex retz. in vivo and in vitro. i. antimicrobial screening. | the hexane extract of heliotropium marifolium yielded a mixture of triterpenoids: beta-sitosterol, stigmasterol, beta-amyrin, friedelan-3beta-ol (epifriedelenol), cycloartenone, beta-amyrin acetate, friedelin and epifriedenyl acetate. isolated triterpenoid and reference antibiotics (gentamycin/mycostatin) were tested against selected pathogenic bacteria and fungi, e.g. escherichia coli, staphylococcus aureus, aspergillus niger and penicillium chrysogenum. the inhibition zone (iz) and the activit ... | 2001 | 11351358 |
| in vitro activities of terbinafine against aspergillus species in comparison with those of itraconazole and amphotericin b. | compared with the in vitro activities of itraconazole (geometric mean mic [gm], 0.56 microg/ml) and amphotericin b (gm, 0.66 microg/ml), the in vitro activity of terbinafine was inferior against aspergillus fumigatus (gm, 19.03 microg/ml) (p < 0.05) and superior against a. flavus (gm, 0.10 microg/ml), a. terreus (gm, 0.16 microg/ml), and a. niger (gm, 0.19 microg/ml). clinical correlation is required, as trailing endpoints are problematic. | 2001 | 11353643 |
| cloning of the aspergillus niger pmra gene, a homologue of yeast pmr1, and characterization of a pmra null mutant. | the pmra gene, a yeast pmr1 homologue, was isolated from aspergillus niger. sequence analysis of the pmra cdna and the genomic dna revealed that two introns exist in the coding region, and that an open reading frame in the cdna encodes a polypeptide of 1056 amino acids containing all the conserved regions present in p-type ca2+-atpases. the predicted pmra protein exhibited a high degree of sequence similarity to the pmr1 proteins from yeasts and mammalians (50-59% identity). the expression of th ... | 2001 | 11356574 |
| cloning and characterization of aspergillus niger genes encoding an alpha-galactosidase and a beta-mannosidase involved in galactomannan degradation. | alpha-galactosidase (ec 3.2.1.22) and beta-mannosidase (ec 3.2.1.25) participate in the hydrolysis of complex plant saccharides such as galacto(gluco)mannans. here we report on the cloning and characterization of genes encoding an alpha-galactosidase (aglc) and a beta-mannosidase (mnda) from aspergillus niger. the aglc and mnda genes code for 747 and 931 amino acids, respectively, including the eukaryotic signal sequences. the predicted isoelectric points of aglc and mnda are 4.56 and 5.17, and ... | 2001 | 11358516 |
| molecular cloning of cdna encoding alpha-n-acetylgalactosaminidase from acremonium sp. and its expression in yeast. | alpha-n-acetylgalactosaminidase (alpha-galnac-ase; ec 3.2.1.49) is an exoglycosidase specific for the hydrolysis of terminal alpha-linked n-acetylgalactosamine in various sugar chains. the cdna, naga, encoding alpha-galnac-ase from acremonium sp. was cloned, sequenced, and expressed in yeast saccharomyces cerevisiae. the naga contains an open reading frame which encodes for 547 amino acid residues including 21 residues of a signal peptide in its n-terminal. the calculated molecular mass of matur ... | 2000 | 11368317 |
| improvement in protein utilization in nursing-home patients on tube feeding supplemented with an enzyme product derived from aspergillus niger and bromelain. | 2001 | 11369177 | |
| microbial hydroxylation and reduction of the diterpene psiadin. | microbial bioconversion studies conducted on the diterpene psiadin have revealed that it was metabolized by aspergillus niger (nrrl 2295) to give 2alpha-hydroxydeoxopsiadin, cunninghamella blakesleeana (atcc 8688a) to give 11beta-hydroxypsiadin, and cylindrocephalum aureum (atcc 12720), gongronella butleri (atcc 22822), kloeckera africana (atcc 20111), and kluyveromyces marxianus var. lactis (atcc 2628) to yield 7alpha-hydroxypsiadin. their structures have been established on the basis of spectr ... | 2001 | 11371011 |
| nafuredin, a novel inhibitor of nadh-fumarate reductase, produced by aspergillus niger ft-0554. | a novel compound, nafuredin, was isolated as an inhibitor of anaerobic electron transport (nadh-fumarate reductase). it was obtained from culture broth of aspergillus niger ft-0554 isolated from a marine sponge. the structure was elucidated as an epoxy-delta-lactone with an attached methylated olefinic side chain on the basis of spectral analysis. | 2001 | 11372780 |
| identification of aspergillus fumigatus and related species by nested pcr targeting ribosomal dna internal transcribed spacer regions. | aspergillus fumigatus is the most common species that causes invasive aspergillosis. in order to identify a. fumigatus, partial ribosomal dna (rdna) from two to six strains of five different aspergillus species was sequenced. by comparing sequence data from genbank, we designed specific primer pairs targeting rdna internal transcribed spacer (its) regions of a. fumigatus. a nested pcr method for identification of other a. fumigatus-related species was established by using the primers. to evaluat ... | 2001 | 11376067 |
| concurrent production of chitin from shrimp shells and fungi. | crustacean shells constitute the traditional and current commercial source of chitin. conversely, the control of fungal fermentation processes to produce quality chitin makes fungal mycelia an attractive alternative source. therefore, the exploitation of both of these sources to produce chitin in a concurrent process should be advantageous and is reported here. three proteolytic aspergillus niger (strains 0576, 0307 and 0474) were selected from a screening for protease activity from among 34 zyg ... | 2001 | 11376610 |
| inhibition of polygalacturonase from verticillium dahliae by a polygalacturonase inhibiting protein from cotton. | an extracellular endo-polygalacturonase (pgase) [e.c. 3.2.1.15] was isolated from 18-day-old culture filtrates of verticillium dahliae and partially purified using gel permeation chromatography. the band responsible for pgase activity was electrophoretically characterized as having a molecular mass of approximately 29 500 and an isoelectric point of 5.4. kinetic studies indicate a km of 3.3 mg ml(-1) and vmax of 0.85 micromol reducing units min(-1) ml(-1) with polygalacturonic acid as substrate. ... | 2001 | 11382229 |
| purification and characterisation of a galactoglucomannan from kiwifruit (actinidia deliciosa). | a galactoglucomannan (ggm) has been purified from the primary cell walls of ripe kiwifruit. a combination of barium hydroxide precipitation, anion exchange- and gel-permeation chromatography gave a chemically homogeneous polymer with a 1:2:2 galactose-glucose-mannose ratio and a molecular weight range of 16-42 kda. complete hydrolysis of the polymer with endo-1,4-beta-mannanase (ec 3.2.1.78) from aspergillus niger gave a mixture of oligosaccharides, three of which (ii, iii, iv) accounted for mor ... | 2001 | 11383899 |