Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| production of an emetic toxin, cereulide, is associated with a specific class of bacillus cereus. | the emetic toxin (cereulide) of bacillus cereus was quantified in several isolates of b. cereus and in various food sources. when the emetic toxin was produced, vomiting-type food poisoning was observed in humans. we also found that the h-1 serovar phenotype was strongly associated with the production of cereulide and that none of the isolates that hydrolyzed starch or expressed diarrheal enterotoxin activity produced cereulide. | 1996 | 8661692 |
| phosphatidylinositol phospholipase c is activated allosterically by the aminoglycoside g418. 2-deoxy-2-fluoro-scyllo-inositol-1-o-dodecylphosphonate and its analogs inhibit glycosylphosphatidylinositol phospholipase c. | phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus is inhibited by myo-inositol-1-o-dodecylphosphonate (ins-1-o-dodecylphosphonate) (morris, j. c., ping-sheng, l., shen, t. y., and mensa-wilmot, k.(1995) j. biol. chem. 270, 2517-2524). a set of novel fluorinated 2-deoxy-ins-1-o-dodecylphosphonates were tested against pi-plc, with potent competitive inhibition by 2-deoxy-2-fluoro-scyllo-ins-1-o-dodecylphosphonate (vp-616l) (xi(50) = 0.09). 2-deoxy-2-fluoro-myo-ins-1-o-dod ... | 1996 | 8663028 |
| [germicidal activity of the detergent virkon]. | 1995 | 8663963 | |
| [sporicidal activity of virkon]. | 1995 | 8663964 | |
| glycosylphosphatidylinositol-alkaline phosphatase from calf intestine as substrate for glycosylphosphatidylinositol-specific phospholipases--microassay using hydrophobic chromatography in pipet tips. | an electrophoretically homogeneous glycosylphosphatidylinositol- alkaline phosphatase fraction from calf intestine, obtained by hydrophobic chromatography, was used as "enzyme-labeled" substrate for testing phospholipase activity. the reaction products were separated by (i) hydrophobic chromatography in pipet tips and (ii) triton x-114 phase partitioning. the chromatographic method presented permits high test frequencies, does not need temperature-controlled sample handling, and is only slightly ... | 1995 | 8678326 |
| use of cluster and discriminant analyses to compare rhizosphere bacterial communities following biological perturbation | we present an approach to comparing the diversity and composition of bacterial communities from different habitats and for identifying which members of a community are most affected by an introduced bacterium. we use this method to explore both previously published and new data from field and growth chamber experiments in which we isolated heterotrophic bacteria from samples of root-free soil, roots of nontreated soybean seedlings, and from the roots of soybean seedlings grown from bacillus cere ... | 1996 | 8688005 |
| food-borne disease outbreaks in taiwan, 1994. | in 1994, 102 outbreaks of food-borne disease involving 4,726 cases were reported to the taiwan department of health. this is the highest number of outbreaks and cases in recent years in taiwan. of these outbreaks, 72.5% (74/102) were caused by bacterial pathogens, with vibrio parahaemolyticus responsible for 56.7% (42/74), staphylococcus aureus 20.3% (15/74), bacillus cereus 14.9% (11/74) and salmonella spp other than s. typhi and s. paratyphi 8.1% (6/74). v. parahaemolyticus has been a leading ... | 1996 | 8688712 |
| microbial counts and characteristics of streptoverticillium strains isolated from soils in the jordan valley. | a microbial survey of total bacterial count, agrobacteria, streptomycetes, and fungi was carried out in six locations in the jordan valley. the highest microbial counts for the four populations were in spring, the lowest mostly in autumn. no significant differences at p < 0.05 were observed either in the counts between the six locations, or in the seasonal counts of agrobacteria and streptomycetes between the six locations as well as within each location. the total bacterial counts (at p < 0.01) ... | 1995 | 8689089 |
| increased accommodation of nascent rna in a product site on rna polymerase ii during arrest. | rna polymerases encounter specific dna sites at which rna chain elongation takes place in the absence of enzyme translocation in a process called discontinuous elongation. for rna polymerase ii, at least some of these sequences also provoke transcriptional arrest where renewed rna polymerization requires elongation factor sii. recent elongation models suggest the occupancy of a site within rna polymerase that accommodates nascent rna during discontinuous elongation. here we have probed the exten ... | 1996 | 8692922 |
| seasonal variations in the isolation of salmonella typhimurium, salmonella enteritidis, bacillus cereus and clostridium perfringens from environmental samples. | calf carcasses contaminated with s. typhimurium, b. cereus and cl. perfringens were placed in either a decomposition pit or in a deep burial pit. salmonella was isolated from the soil around the decomposition pit for 27 weeks and for 15 weeks around the burial site. salmonella re-appeared in soil samples during cold winter weather after an apparent 9-week absence from the decomposition pit and after 68 weeks in the burial site (a total of 88 weeks after the start of the experiment). there was al ... | 1996 | 8693843 |
| use of site-directed mutagenesis to probe structure-function relationships of alpha-toxin from clostridium perfringens. | the nh2-terminal domain of the alpha-toxin of clostridium perfringens is highly homologous to the complete phospholipase c from bacillus cereus (pc-plc), for which a high-resolution crystal structure is available. this structural information was used as the basis of a site-directed mutagenesis strategy in which critical amino acid residues of alpha-toxin involved in zinc binding, interaction with substrate, or catalysis were replaced. biochemical studies with the corresponding toxin variants ind ... | 1996 | 8698464 |
| (all trans)-2,4,6,8,10,12-tetradecahexene-1,14-dial, a new pigment from conidiobolus paulus. | a study of the coloring matter produced by conidiobolus paulus drechsler nrrl 2648 on potato-dextrose medium led to the isolation of a new dialdehyde unsaturated metabolite, (all trans)-2,4,6,8,10,12-tetradeca-1,14-hexenedial. the structure was characterized by ms and by 1h- and 13c-nmr. the compound inhibited the growth of the gram-positive bacterium, bacillus cereus, and the gram-negative bacterium, escherichia coli (mic values of 10 micrograms and 50 micrograms, respectively). | 1996 | 8699189 |
| production of kanosamine by bacillus cereus uw85. | bacillus cereus uw85 produces two antibiotics that contribute to its ability to suppress certain plant diseases (l. silo-suh, b. lethbridge, s. j. raffel, h. he, j. clardy, and j. handelsman, appl. environ. microbiol. 60:2023-2030, 1994). to enhance the understanding of disease suppression by uw85, we determined the chemical structure, regulation, and the target range of one of the antibiotics. the antibiotic was identified as 3-amino-3-deoxy-d-glucose, also known as kanosamine. kanosamine was h ... | 1996 | 8702302 |
| inhibition by gangliosides of bacillus cereus phospholipase c activity against monolayers, micelles and bilayer vesicles. | the effect of complex glycosphingolipids (gangliosides) on the activity of phospholipase c from bacillus cereus was studied using lipid monolayers, mixed micelles and small unilamellar vesicles containing phosphatidylcholine as substrate. in all artificial membrane systems assayed, gangliosides exhibit qualitatively similar inhibitory properties. gangliosides decrease the enzyme activity irrespective of the aggregation structure in which the substrate is offered to b. cereus phospholipase c, and ... | 1996 | 8706693 |
| n-(2-ferrocene-ethyl)maleimide: a new electroactive sulphydryl-specific reagent for cysteine-containing peptides and proteins. | we report the synthesis and application of a specific electroactive label, n-(2-ferrocene-ethyl)maleimide, which provides new redox properties to organic compounds and proteins possessing sulphydryl groups. its reaction conditions with the cysteine-containing peptide, glutathione, and a terminal monooxygenase enzyme, cytochrome p450cam are presented. the labelled peptide and enzyme acquired reversible electrochemical properties due to the attached ferrocene moiety. | 1996 | 8706845 |
| microbiological quality & incidence of organisms of public health importance in food & water in ludhiana. | bacteriological analysis of 713 samples of various types of foods and related articles and potable water samples from different places in ludhiana, punjab was carried out. the highest counts ranging from 2.5 x 10(6)-7.5 x 10(8) organisms/g were observed in raw vegetables and fruits, followed by 3 x 10(6)-9.8 x 10(7)/ml, 8.3 x 10(4)-8.9 x 10(7)/g and 1 x 10(3)-6.7 x 10(7)/g in fruit juice, milk and its products, and salty/non milk snacks respectively. fresh chapati, dal, rice, cooked vegetables a ... | 1996 | 8707360 |
| [bacillus cereus chitinases: isolation and characteristics]. | three chitinases (m(r) = 68, 52 and 38 kda) have been isolated from the cultural filtrate of bacillus cereus strain vkpm b-6838 by stepwise hydrophobic chromatography on butyl-toyopearl and gel filtration on superdex 75 (fplc). the chitinases are stable in the ph range 4-10 and have the same ph optimum of activity. the 68 and 38 kda enzymes display the highest activity at 60 degrees c. while the 52 kda chitinase-at 50 degrees c. in contrast with the 68- and 52 kda enzymes, the 38 kda chitinase h ... | 1996 | 8717500 |
| the role of sh and s-s groups in bacillus cereus beta-amylase. | the properties of sulfhydryl (sh) and disulfide (s-s) groups in bacillus cereus bq10-s1 spo iii beta-amylase have been investigated to clarify their roles in the enzyme action. two out of three cysteine residues in b. cereus beta-amylase were found to form an s-s bond, which was found to be located between cys91 and cys99 by the analysis of an s-s containing peptide. the replacement of the soybean beta-amylase model around l3 loop 1 revealed that the s-s bond is located at the root of this flexi ... | 1995 | 8720125 |
| the antimicrobial properties of chile peppers (capsicum species) and their uses in mayan medicine. | a survey of the mayan pharmacopoeia revealed that tissues of capsicum species (solanaceae) are included in a number of herbal remedies for a variety of ailments of probable microbial origin. using a filter disk assay, plain and heated aqueous extracts from fresh capsicum annuum, capsicum baccatum, capsicum chinese, capsicum frutescens, and capsicum pubescens varieties were tested for their antimicrobial effects with fifteen bacterial species and one yeast species. two pungent compounds found in ... | 1996 | 8735449 |
| [bacteremia associated with implantable device for central venous access caused by bacillus cereus]. | 1996 | 8744378 | |
| diarrhoeal enterotoxin production by strains of bacillus thuringiensis isolated from commercial bacillus thuringiensis-based insecticides. | strains of bacillus cereus and b. thuringiensis were tested by the tecra via kit for the ability to produce a diarrhoeal enterotoxin. the strains of b. thuringiensis were isolated from commercial b. thuringiensis-based insecticides (bactimos, dipel, florbac, fc, foray 48b, novodor fc, turex, vectobac, xentari). the production of diarrhoeal enterotoxin varied by a factor of more than 100 among the different strains tested. b. cereus (f4433/73) produced the highest amount of enterotoxin and the b. ... | 1995 | 8745010 |
| what problems does the food industry have with the spore-forming pathogens bacillus cereus and clostridium perfringens? | spore-forming bacteria are special problems for the food industry. it is not always possible to apply enough heat during food processing to kill spores, thus we have to take advantage of knowledge of the spore-formers to control them. for the meat industry clostridium perfringens might become a special problem, although this bacterium mainly causes food poisoning through food served in restaurants, hospitals or homes for elderly people (cliver, 1987; reynolds, 1987; gondrosen et al., 1990). the ... | 1995 | 8750663 |
| probes and polymerase chain reaction for detection of food-borne bacterial pathogens. | dna-hybridization and the polymerase chain reaction (pcr) are techniques commonly used to detect pathogenic bacteria. in this paper, the use of these techniques for detection of salmonella, e. coli, v. cholerae, non-o1 vibrio, yersinia enterocolitica, campylobacter, listeria monocytogenes, staphylococcus aureus, bacillus cereus, clostridium perfringens, and c. botulinum is reviewed with emphasis on application in food microbiology. in food control, dna-techniques have most often been used in a ' ... | 1995 | 8751091 |
| a germination-specific spore cortex-lytic enzyme from bacillus cereus spores: cloning and sequencing of the gene and molecular characterization of the enzyme. | a gene (sleb) encoding a 24-kda germination-specific spore cortex-lytic enzyme, probably an n-acetylmuramyl-l-alanine amidase, was cloned from bacillus cereus, and its nucleotide sequence was determined. it was indicated that the enzyme is produced as a 259-residue protein with a signal sequence of 32 residues and is present in dormant spores in its active form. sulfhydryl reagents inactivated the enzyme, but mutation of a single cysteine of the protein, cys-258, to gly did not cause complete in ... | 1996 | 8752358 |
| crystal structure of phosphatidylinositol-specific phospholipase c from bacillus cereus in complex with glucosaminyl(alpha 1-->6)-d-myo-inositol, an essential fragment of gpi anchors. | numerous proteins on the external surface of the plasma membrane are anchored by glycosylated derivatives of phosphatidylinositol (gpi), rather than by hydrophobic amino acids embedded in the phospholipid bilayer. these gpi anchors are cleaved by phosphatidylinositol-specific phospholipases c (pi-plcs) to release a water-soluble protein with an exposed glycosylinositol moiety and diacylglycerol, which remains in the membrane. we have previously determined the crystal structure of bacillus cereus ... | 1996 | 8755729 |
| selective killing of human monocytes and cytokine release provoked by sphingomyelinase (beta-toxin) of staphylococcus aureus. | the best-known activity of staphylococcus aureus sphingomyelinase c, alias beta-toxin, is as a hemolysin that provokes hot-cold lysis of erythrocytes which contain substantial amounts of sphingomyelin in the plasma membrane. sheep erythrocytes are most susceptible, and we found that one hemolytic unit, representing the toxin concentration that elicits 50% hemolysis of 2.5 x 10(8) erythrocytes per ml, corresponds to 0.05 enzyme units or to approximately 0.25 microg of sphingomyelinase per ml. the ... | 1996 | 8757823 |
| [construction of shuttle vector containing delta-endotoxin gene of bacillus thuringiensis]. | the bacillus thuringiensis toxin gene cryia(c) was inserted into the shuttle vector pbe-2 to construct pamy for expressing the b.t. gene in both gram-negative and -positive bacterial systems. pamy was introduced into wild type bacillus cereus, b.brevis and b.subtilis by electroporation. transformants containing delta-endotoxin gene produced proteins reacted with b.t. crystal protein antibody. upon biological toxicity tests, the transformants gave a mortality of 100% against ostrinia furnacilis, ... | 1996 | 8762474 |
| zwittermicin a resistance gene from bacillus cereus. | zwittermicin a is a novel aminopolyol antibiotic produced by bacillus cereus that is active against diverse bacteria and lower eukaryotes (l.a. silo-suh, b.j. lethbridge, s.j. raffel, h. he, j. clardy, and j. handelsman, appl. environ. microbiol. 60:2023-2030, 1994). to identify a determinant for resistance to zwittermicin a, we constructed a genomic library from b. cereus uw85, which produces zwittermicin a, and screened transformants of escherichia coli dh5alpha, which is sensitive to zwitterm ... | 1996 | 8763956 |
| evidence for a further enterotoxin complex produced by bacillus cereus. | out of 321 strains of bacillus cereus from several sources and isolated in four different countries, 239 (74%) produced cytotoxins. only 127 (53%) of the cytotoxic strains were positive for the b-component gene of the haemolysin bl (enterotoxin) by polymerase chain reaction (pcr). western blots using antiserum produced against enterotoxin(s) gave positive results for 199 (83%) of the cytotoxic b. cereus strains. on closer examination of seven of the strains, involved in food poisoning, we found ... | 1996 | 8768515 |
| characterisation of a non-haemolytic enterotoxin complex from bacillus cereus isolated after a foodborne outbreak. | three enterotoxic components have been isolated from a strain of bacillus cereus which was involved in a large food poisoning outbreak in norway in 1995. the components were purified by chromatography on three different columns. three proteins of 39, 45 and 105 kda, respectively, were found to be necessary for maximum cytotoxicity. the amino acid n-terminal sequences of the 39 and 45 kda proteins were determined. the 45 kda component was the same protein as the main antigen detected in the bacil ... | 1996 | 8768516 |
| the chromosome map of bacillus thuringiensis subsp. canadensis hd224 is highly similar to that of the bacillus cereus type strain atcc 14579. | a physical map of the bacillus thuringiensis subsp. canadensis hd224 chromosome based on asci, noti, and sfii restriction sites has been established. the chromosome map of 4.3 mb was similar to a revised map of the chromosome of the b. cereus type strain atcc 14579, except that the b. thuringiensis subsp. canadensis hd224 chromosome lacked a noti site and had two additional asci sites. the positions of 27 probes were identical in the common macromap. a probe for the insecticidal toxin gene, cryi ... | 1996 | 8768518 |
| [the anticomplement activity of the commercial strain of bacillus cereus ip 5832 and of enterobacteria when cocultured]. | bacillus cereus production strain ip 5832 of the preparation "bactisubtil" has been characterized with respect to its anticomplementary activity (aca) and the parameters of its heterogeneity in the process of adaptation to trypticase-soybean medium and during cultivation in anaerobic conditions have been determined. the cultivation of b.cereus strain ip 5832 jointly with escherichia coli, enterobacter cloacae and klebsiella pneumoniae strains, isolated in enteric disbiosis, affects aca in differ ... | 1996 | 8771743 |
| [bacillus cereus meningitis in a patient with human immunodeficiency virus infection]. | 1995 | 8787298 | |
| analysis of the critical sites for protein thermostabilization by proline substitution in oligo-1,6-glucosidase from bacillus coagulans atcc 7050 and the evolutionary consideration of proline residues. | to identify the critical sites for protein thermostabilization by proline substitution, the gene for oligo-1,6- glucosidase from a thermophilic bacillus coagulans strain, atcc 7050, was cloned as a 2.4-kb dna fragment and sequenced. in spite of a big difference in their thermostabilities, b. coagulans oligo-1,6-glucosidase had a large number of points in its primary structure identical to respective points in the same enzymes from a mesophilic bacillus cereus strain, atcc 7064 (57%), and an obli ... | 1996 | 8787404 |
| antifungal azoxybacilin exhibits activity by inhibiting gene expression of sulfite reductase. | azoxybacilin, produced by bacillus cereus, has a broad spectrum of antifungal activity in methionine-free medium and has been suggested to inhibit sulfite fixation. we have further investigated the mode of action by which azoxybacilin kills fungi. the compound inhibited the incorporation of [35s] sulfate into acid-insoluble fractions of saccharomyces cerevisiae under conditions in which virtually no inhibition was observed for dna, rna, or protein synthesis. it did not interfere with the activit ... | 1996 | 8787893 |
| crystal structure of the wide-spectrum binuclear zinc beta-lactamase from bacteroides fragilis. | the metallo-beta-lactamase from bacteroides fragilis hydrolyzes a wide range of beta-lactam antibiotics, and is not clinically susceptible to any known beta-lactamase inhibitors. b. fragilis is associated with post-surgery hospital infections, and there has been a recent report of plasmid-mediated dissemination of the enzyme. effective inhibitors are therefore urgently needed. knowledge of the three-dimensional structure will aid in the drug design effort. | 1996 | 8805566 |
| characteristics of streptomyces strains isolated from soils in two landfill areas in north jordan. | a total of 114 streptomyces strains were isolated and purified from soils collected from al akider (53) and from al kafeer (61) landfill areas in north jordan. the al akider strains were classified into 5 colour series gray (27), white (18), yellow (3), violet (3) and green (2); while the al kafeer strains were classified into 6 colour series white (26), gray (16), yellow (9), red (2), green (7) and blue (1). melanin pigment was produced by 21 and 25% of the al akider and al kafeer strains, resp ... | 1996 | 8806943 |
| infection with bacillus cereus after close-range gunshot injuries. | three patients were admitted with close-range gunshot wounds of the knee and lower leg, inflicted in all three cases through clothing. at admission, all of the patients were given antibiotics (cefuroxime and metronidazole) to prevent streptococcal and anaerobic infection. all of the patients developed severe tissue infection with bacillus cereus within days of admission. in one case, the organism was also recovered from the blood. b. cereus is capable of causing severe infection after trauma and ... | 1996 | 8810981 |
| the aggregation-mediated conjugation system of bacillus thuringiensis subsp. israelensis: host range and kinetics of transfer. | the aggregation-mediated conjugation system in bacillus thuringiensis subsp. israelensis encoded on the plasmid pxo16 is characterized by the formation of aggregates when agr+ and agr- cells are socialized in exponential growth. using the aggregation phenotypes, we have identified potential recipients of the aggregation-plasmid pxo16 among bacillus cereus, bacillus subtilis, bacillus megaterium, bacillus sphaericus, and 24 subspecies of b. thuringiensis. we found 14 agr- strains, i.e., potential ... | 1996 | 8824168 |
| a gene (sleb) encoding a spore cortex-lytic enzyme from bacillus subtilis and response of the enzyme to l-alanine-mediated germination. | the bacillus subtilis sleb gene, which codes for the enzyme homologous to the germination-specific amidase from bacillus cereus, was cloned and its nucleotide sequence was determined. sequence analysis showed that it had an open reading frame of 918 bp, coding for a polypeptide of 305 amino acids with a putative signal sequence of 29 residues. enzyme activity was not found in germination exudate of b. subtilis spores, which differs from the case of b. cereus enzyme. a b. subtilis mutant with an ... | 1996 | 8830707 |
| frequency of antimicrobial resistance and plasmid profiles of bacillus cereus strains isolated from milk. | plasmid profile analysis and susceptibility to eight commonly used antimicrobial agents was done on sixty bacillus cereus (b. cereus) strains isolated from milk. about seventy two percent (71.7%) of the isolates contained plasmids. the number of plasmids per isolate ranged between 1 and 5, while plasmid sizes ranged between 60 and 0.1 megadaltons (mda). all isolates were resistant to ampicillin with a relatively high rate of resistance to cotrimoxazole and sulphamethoxazole. the isolates showed ... | 1996 | 8840598 |
| expression and site-directed mutagenesis of the phosphatidylcholine-preferring phospholipase c of bacillus cereus: probing the role of the active site glu146. | a series of site-specific mutants of the phosphatidylcholine-preferring phospholipase c from bacillus cereus (plcbc) was prepared in which the glutamic acid residue at position 146 was replaced with glutamine, aspartic acid, histidine, and leucine to elucidate what role glu146 might play in catalysis. an expression system for the native enzyme in escherichia coli was first developed to provide plcbc that was fused via an intervening factor xa protease recognition sequence at its n-terminus to ma ... | 1996 | 8841144 |
| reduction of risks associated with fumonisin contamination in corn. | fumonisins, produced by fusarium moniliforme, have been recognized as an important group of chemicals which cause health risks in domestic animals and humans. decontamination procedures for fumonisin b1 (fb1) were evaluated to determine chemical modification and reduction in toxic/carcinogenic potentials. ammoniation, a procedure used for decontamination of aflatoxins, yielded a 79% reduction in fb1 levels in naturally contaminated corn. authentic fb1 and fb1-contaminated corn were exposed to al ... | 1996 | 8850629 |
| antibacterial activity of lactobacillus plantarum ug1 isolated from dry sausage: characterization, production and bactericidal action of plantaricin ug1. | lactobacillus plantarum ug1 isolated from dry sausage produced an antimicrobial substance that inhibited other strains of the genera lactobacillus and lactococcus, and some foodborne pathogens including listeria monocytogenes, bacillus cereus, clostridium perfringens and clostridium sporogenes. this antibacterial substance was inactivated by proteolytic enzymes and showed a bactericidal mode of action. consequently, it was characterized as a bacteriocin, and was designated plantaricin ug1. this ... | 1996 | 8854175 |
| production of diarrheal toxin by selected strains of bacillus cereus. | the production of diarrheal toxin by six selected strains of bacillus cereus was monitored during growth at 32 degrees c, a temperature described as near-optimal for growth and toxin production. toxic activity was measured in culture filtrates and cellular extracts sampled at three different times during growth. two alternative methods, a cytotoxicity test on chinese hamster ovary (cho) cells and a commercial immunological test (bcet-rpla, oxoid) were used. toxin titres were in agreement with ep ... | 1996 | 8854186 |
| preparation and validation of a growth model for bacillus cereus: the effects of temperature, ph, sodium chloride and carbon dioxide. | the growth responses of a vegetative inoculum of bacillus cereus as influenced by varying conditions of temperature, ph value and sodium chloride concentration (% w/v) and carbondioxide concentration (% v/v) were determined in laboratory medium. growth curves in concentrations of nacl in the range 0.5-10.5% (w/v), ph values in the range 4.5-7.0, co2 concentrations in the range 10-80% (v/v) and storage temperatures from 10 degrees c to 30 degrees c were fitted using the regime of baranyi et al. ( ... | 1996 | 8854187 |
| application of predictive microbiology to estimate the number of bacillus cereus in pasteurised milk at the point of consumption. | a procedure is presented to quantitatively estimate the growth of a particular organism in a food product during chilled storage using predictive microbiology. this results in a quantification of the contribution of every individual process step to the total number of organisms, which may be a useful tool to support decisions on existing process lines as well as in process and product design. it is demonstrated that predictive microbiology will only estimate to within orders of magnitude of bact ... | 1996 | 8856374 |
| experimental posttraumatic bacillus cereus endophthalmitis in a swine model. efficacy of intravitreal ciprofloxacin, vancomycin, and imipenem. | the authors compare the intravitreal efficacy of ciprofloxacin, vancomycin and imipenem, in treating experimental bacillus cereus endophthalmitis. | 1996 | 8865392 |
| variable numbers of rrna gene operons in bacillus cereus strains. | ribosomal rna operon organisation was analysed in two bacillus cereus strains of different chromosome size, atcc 10987 (5.4 mb) and f0837/76 (2.4 mb). we estimated that there were twelve and nine copies of the rrna operons in these two strains, respectively. in b. cereus atcc 10987 six rrna operons were less than 10 kb apart, while in b. cereus f0837/76 four rrna operons were similarly clustered. the origin of replication was located in the vicinity of a rrna operon in both strains. | 1996 | 8867386 |
| mechanism and structure based inhibitors of phospholipase c enzymes. | pi-specific plc enzymes are a key component of phosphatidylinositol-mediated signaling pathways since the hydrophobic product, diacylglycerol, activates protein kinase c and the water-soluble product, inositol trisphosphate, is involved in ca2+ mobilization. nonspecific, or pc-plc, enzymes can generate diacylglycerol without ca2+ mobilization. a series of inhibitors, both lipophilic and water-soluble, have been synthesized to target each of these two classes of plc enzymes. design of the inhibit ... | 1996 | 8869740 |
| methods for increasing survivability during storage of exponentially growing bacteria. | a protocol has been developed for storing gram (-) bacterial cells at 0 degrees c, which allows greater than 90% of stored cells to retain colony-forming ability for up to 60 days. the protocol, which yields essentially identical results when used with escherichia coli or pseudomonas aeruginosa, does not enhance survivability of bacillus cereus. the greatest and longest survival is enjoyed when exponentially growing cells in minimal-glucose medium are deprived of carbon for about 9 h, supplement ... | 1996 | 8875912 |
| in-vitro activity of ramoplanin (a novel lipoglycopeptide), vancomycin, and teicoplanin against gram-positive clinical isolates from cancer patients. | the in-vitro activities of ramoplanin, vancomycin, and teicoplanin against gram-positive organisms isolated from cancer patients were determined. ramoplanin was the most active agent tested inhibiting all isolates at a concentration of < or = 0.5 mg/l. although all isolates were also susceptible to vancomycin and teicoplanin, their activities were surpassed by that of ramoplanin. the activity of teicoplanin was moderately better than that of vancomycin against bacillus cereus, enterococcus faeci ... | 1996 | 8877541 |
| inhibition of phosphatidylinositol-specific phospholipase c: studies on synthetic substrates, inhibitors and a synthetic enzyme. | enzyme inhibition studies on phosphatidylinositol-specific phospholipase c (pi-plc) from b. cereus were performed in order to gain an understanding of the mechanism of the pi-plc family of enzymes and to aid inhibitor design. inhibition studies on two synthetic cyclic phosphonate analogues (1,2) of inositol cyclic-1:2-monophosphate (cip), glycerol-2-phosphate and vanadate were performed using natural phosphatidylinositol (pi) substrate in triton x100 co-micelles and an nmr assay. further inhibit ... | 1996 | 8877813 |
| evaluation of serotyping, biotyping, plasmid banding pattern analysis, and hep-2 vacuolation factor assay in the epidemiological investigation of bacillus cereus emetic-syndrome food poisoning. | to assess the value of the plasmid banding patterns, the vacuolation factor (vf) assay, biotyping, and serological typing as epidemiological markers for strains of bacillus cereus causing emetic-syndrome illness, 43 isolates from five outbreaks and an additional 76 strains isolated in food-poisoning outbreaks caused by other enteric pathogens were examined by these techniques, and the results were compared. thirty-eight (88%) of the 43 outbreak strains produced vacuolation responses in hep-2 cel ... | 1996 | 8880304 |
| randomly amplified polymorphic dna (rapd) assay for genomic fingerprinting of bacillus cereus isolates. | randomly amplified polymorphic dna (rapd) assay was used for epidemiological subtyping of b. cereus and b. lentus. within 25 isolates of b. cereus up to 22 strain types could be determined when five primers were used. rapd patterns, which were found in three b. lentus strains, clearly differed form those of b. cereus. the rapd technique proved to be an effective tool for the characterization of b. cereus strains. | 1996 | 8880317 |
| effect of ph of the recovery medium on the apparent heat resistance of three strains of bacillus cereus. | the influence of ph of the recovery medium, in the range 7.6-5.4, on the apparent heat resistance of three strains of bacillus cereus (atcc 4342, 7004 and 9818) has been investigated. the highest counts of heat-injured spores were obtained at ph near neutral, decreasing markedly as ph was reduced, especially with longer heating times. when the media were acidified, the apparent d-values tended to decrease, although some exceptions related to the strain and the nature of the medium were observed. ... | 1996 | 8880321 |
| investigation of bacterial resistance to hatchery disinfectants. | three commercial chicken hatcheries were sampled for environmental bacteria. isolated bacteria were tested for resistance to commercial preparations of quaternary ammonia, phenolic, and glutaraldehyde liquid disinfectants. bacterial isolates were exposed to several disinfectant dilutions bracketing the dilutions recommended by the manufacturer for 5-, 10-, and 15-min exposure periods before subculturing to broth medium. approximately 8% of the isolates from two of three hatcheries were resistant ... | 1996 | 8883778 |
| subtyping of bacillus cereus by total cell protein patterns and arbitrary primer polymerase chain reaction. | bacillus cereus is a ubiquitous sporeforming gram-positive rod that is associated with foodborne outbreaks as well as several opportunistic infections. inspite of the prevalence of b. cereus associated foodborne outbreaks, subtyping of the species using molecular typing assays was not attempted. in this study we have recovered 58 b. cereus isolates from natural and clinical sources and initially characterized them, along with a b. cereus strain (atcc 14579) and b. thuringiensis natural isolate, ... | 1996 | 8884200 |
| synthesis, structure-activity relationships, and the effect of polyethylene glycol on inhibitors of phosphatidylinositol-specific phospholipase c from bacillus cereus. | substrate analog inhibitors of bacillus cereus phosphatidylinositol-specific phospholipase c (pi-plc) were synthesized and screened for their suitability to map the active site region of the enzyme by protein crystallography. analogs of the natural substrate phosphatidylinositol (pi) were designed to examine the importance of the lipid portion and the inositol phosphate head group for binding to the enzyme. the synthetic compounds contained pentyl, hexyl, or hexanoyl and octyl lipid chains at th ... | 1996 | 8893831 |
| structure-activity relationships in a series of substituted indolocarbazoles: topoisomerase i and protein kinase c inhibition and antitumoral and antimicrobial properties. | a series of compounds structurally related to staurosporine, rebeccamycin, and corresponding aglycones was synthesized, and their activities toward protein kinase c and topoisomerases i and ii were tested together with their in vitro antitumor efficiency against murine b16 melanoma and p388 leukemia cells. their antimicrobial activities were also examined against a gram-negative bacterium (escherichia coli), a yeast (candida albicans), and three gram-positive bacteria (bacillus cereus, streptomy ... | 1996 | 8893841 |
| epidemiological typing of bacillus spp. isolated from food. | biotypes, fatty acid profiles, and restriction fragment length polymorphisms of a pcr product (pcr-rflp of the cereolysin ab gene) were compared for 62 isolates of the bacillus cereus group. eleven isolates originated from various foods, and 51 isolates were obtained from pasteurized milk which had been processed by two different dairies. the isolates were clustered into 6 biotypes, 10 fatty acid groups, or 7 pcr-rflp clusters. isolates with mesophilic or psychrotrophic characteristics were pref ... | 1996 | 8900016 |
| inhibition of microbial growth by ajoene, a sulfur-containing compound derived from garlic. | ajoene, a garlic-derived sulfur-containing compound that prevents platelet aggregation, exhibited broad-spectrum antimicrobial activity. growth of gram-positive bacteria, such as bacillus cereus, bacillus subtilis, mycobacterium smegmatis, and streptomyces griseus, was inhibited at 5 micrograms of ajoene per ml. staphylococcus aureus and lactobacillus plantarum also were inhibited below 20 micrograms of ajoene per ml. for gram-negative bacteria, such as escherichia coli, klebsiella pneumoniae, a ... | 1996 | 8900018 |
| synthesis and reactions of some new 4h-pyrano[3,2-c]benzopyran-5-one derivatives and their potential biological activities. | several new 4h-pyrano[3,2-c]benzopyran-5-ones (6a-g and 10) which exhibited antibacterial and fungicidal activity, have been synthesized via a one flask multicomponent condensation of aldehydes with malononitrile and 4-hydroxycoumarin. the reactions of 6a with several nucleophilic reagents are reported. | 1996 | 8900865 |
| characterization of pcr products from bacilli using electrospray ionization fticr mass spectrometry. | a procedure for rapid purification of polymerase chain reaction (pcr) products allowing precise molecular weight determination using electrospray ionization-fourier transform ion cyclotron resonance (esi-fticr) mass spectrometry is described. pcr amplification utilized the dna polymerase from pyrococcus furiosus (pfu) which, unlike taq, does not incorporate a nontemplated terminal deoxyadenosine phosphate. an 89-base pair nucleotide portion of the spacer region between the 16s and 23s ribosomal ... | 1996 | 8914480 |
| comparison of the beta-toxins from staphylococcus aureus and staphylococcus intermedius. | the beta-toxins produced by staphylococcus aureus and staphylococcus intermedius were purified to homogeneity from culture supernatants. although the toxin from s. aureus has been throughly studied, less is known about its unique counterpart from s. intermedius. this is the first reported purification and analysis of the s. intermedius beta-toxin. both toxins have similar enzymatic properties, belong to the class of neutral sphingomyelinases c, and have a high specificity for sphingomyelin. they ... | 1996 | 8914839 |
| mouse lethal activity of a hep-2 vacuolation factor, cereulide, produced by bacillus cereus isolated from vomiting-type food poisoning. | the hep-2 vacuolation factor (or cereulide) produced by bacillus cereus isolated from vomiting-type food poisoning, which is supposed to induce emesis, was found to give mouse and suncus lethality after intravenous and intraperitoneal administration. the emetic activity of the factor was also found to be resistant to heating at 121 degrees c for 15 min, exposure to ph 2 and 11, and to digestion with proteolytic enzymes such as pepsin and trypsin. these findings suggest that the cereulide produce ... | 1996 | 8916007 |
| killing of microorganisms by pulsed electric fields. | lethal effects of pulsed electric fields (pef) on suspensions of various bacteria, yeast, and spores in buffer solutions and liquid foodstuffs were examined. living-cell counts of vegetative cell types were reduced by pef treatment by up to more than four orders of magnitude (> 99.99%). on the other hand, endo- and ascospores were not inactivated or killed to any great extent. the killing of vegetative cell types depends on the electrical field strength of the pulses and on the treatment time (t ... | 1996 | 8920190 |
| evaluation of swirling, ph, and glucose tests for the detection of bacterial contamination in platelet concentrates. | although infrequent, episodes of transfusion-associated bacterial sepsis may lead to serious outcomes or death and therefore are of concern. this study evaluates the sensitivity of three surrogate tests for the presence of bacteria in platelet concentrates: cessation of swirling, low extracellular ph, and low plasma glucose levels. | 1996 | 8937410 |
| is the exploding powder gas of the propellant from blank cartridges sterile? | shots from blank weapons loaded with blank cartridges, when fired from close range or as a contact shot, almost always cause the skin to burst open and lead to injuries to structures below the surface. subsequently, wound infections are often observed. in addition to the introduction of skin germs, the possibility exists that contaminated propellants may enter into consideration as a source of infection. using step-by-step experimental procedures we were able to demonstrate that: 1. blank cartri ... | 1996 | 8939008 |
| an alkaline d-stereospecific endopeptidase with beta-lactamase activity from bacillus cereus. | we purified a novel extracellular d-stereospecific endopeptidase, alkaline d-peptidase (d-stereospecific peptide hydrolase, ec 3.4.11.-), to homogeneity from the culture broth of the soil bacterium bacillus cereus strain df4-b. the mr of the enzyme was 37,952, and it was composed of a single polypeptide chain. the optimal ph for activity was approximately 10.3. the enzyme was strictly d-stereospecific toward oligopeptides composed of dphenylalanine such as (d-phe)3 and (d-phe)4. the enzyme also ... | 1996 | 8939979 |
| vanadate is a potent competitive inhibitor of phospholipase c from bacillus cereus. | monomeric vanadate is a potent competitive inhibitor of phospholipase c from bacillus cereus, much better than other oxyanions (e.g., phosphate or iodate). the apparent efficiency of inhibition depends on the substrate aggregate structure. the measured inhibition constant with respect to monomeric phosphatidylcholine substrate is 0.21 mm under conditions where the k(m) is 0.12 mm; for micellar substrate the apparent ki appears much lower and in fact tracks the apparent k(m) which decreases 10-fo ... | 1996 | 8948489 |
| experimental bacillus cereus post-traumatic endophthalmitis and treatment with ciprofloxacin. | bacillus species remain an important cause of post-traumatic endophthalmitis, often causing permanent visual loss. | 1996 | 8949723 |
| origin of the lag period in the phospholipase c cleavage of phospholipids in membranes. concomitant vesicle aggregation and enzyme activation. | when phospholipase c is added to a suspension of large unilamellar vesicles of egg phosphatidylcholine, maximal rates of hydrolysis occur only after a latency period. no lag period is seen when the substrate is in the form of small (sonicated) vesicles, or of short-chain phosphatidylcholine monomers. for a given vesicle concentration, the lag time may vary as a function of ca2+, enzyme concentration, or temperature, but activation occurs at a fixed molar fraction of diacylglycerol produced. lag ... | 1996 | 8952465 |
| mobilization of "nonmobilizable" plasmids by the aggregation-mediated conjugation system of bacillus thuringiensis. | the aggregation-mediated conjugation system of bacillus thuringiensis subsp, israelensis (bti), encoded by the 200-kb plasmid pxo16, is highly potent in transferring itself and efficient in mobilizing other nonconjugative plasmids. in the present study we have analyzed the native bacillus cereus plasmid pbc16. this plasmid has previously been shown to harbor a mob gene (orf beta) and a locus functioning as an orit site in plasmid pls20-mediated conjugation in bacillus subtilis. however, in the c ... | 1996 | 8954879 |
| polyclonal catalytic anti-idiotypic antibodies with a beta-lactamase activity. | 1996 | 8958088 | |
| mechanism of action of hemolysin iii from bacillus cereus. | bacillus cereus hemolysin iii activity was tested in crude extracts, from escherichia coli carrying the hly-iii gene. it was concluded that hemolysin iii is a pore-forming hemolysin with functional pore diameter of about 3-3.5 nm. hemolysis occurs in at least three steps: (i) the temperature-dependent binding of the hly-iii monomers to the erythrocyte membrane; (ii) the temperature-dependent formation of the transmembrane oligomeric pore; (iii) the temperature-independent erythrocyte lysis. | 1996 | 8962879 |
| cloning of the glutamine synthetase gene from group b streptococci. | the glna gene from the human pathogen streptococcus agalactiae was cloned from a genomic library prepared with the lambda phage vector lambdadashii. a 4.6-kb dna fragment of one of the recombinant phages was subcloned in puc18. this escherichia coli clone expressed a 52-kda protein encoded by a 1,341-bp open reading frame. the nucleotide sequence of the open reading frame and the deduced amino acid sequence shared a significant degree of homology with the sequences of other glutamine synthetases ... | 1997 | 8975911 |
| a distant evolutionary relationship between bacterial sphingomyelinase and mammalian dnase i. | the three-dimensional structure of bacterial sphingomyelinase (smase) was predicted using a protein fold recognition method; the search of a library of known structures showed that the smase sequence is highly compatible with the mammalian dnase i structure, which suggested that smase adopts a structure similar to that of dnase i. the amino acid sequence alignment based on the prediction revealed that, despite the lack of overall sequence similarity (less than 10% identity), those residues of dn ... | 1996 | 8976554 |
| inhibition of metallo-beta-lactamases by a series of mercaptoacetic acid thiol ester derivatives. | a series of mercaptoacetic acid thiol esters have been identified as metallo-beta-lactamase inhibitors. electrospray mass spectrometry (esms) has shown that irreversible inhibition of the bacillus cereus ii metallo-beta-lactamase by sb214751, sb214752, and sb213079 was concomitant with a 90-da increase in mass of the enzyme. tryptic digestion of the b. cereus ii inhibited with sb214751 illustrated that the peptide fragment, containing the only cysteine of the enzyme, had undergone a mass increme ... | 1997 | 8980769 |
| effects of inactivation or overexpression of the sspf gene on properties of bacillus subtilis spores. | inactivation of the bacillus subtilis sspf gene had no effect on sporulation, spore resistance, or germination in a wild-type strain or one lacking dna protective alpha/beta-type small, acid-soluble proteins (sasp). overexpression of sspf in wild-type spores or in spores lacking major alpha/beta-type sasp (alpha- beta- spores) had no effect on sporulation but slowed spore outgrowth and restored a small amount of uv and heat resistance to alpha- beta- spores. in vitro analyses showed that sspf is ... | 1997 | 8982008 |
| cloning, sequencing, and expression of a beta-amylase gene from bacillus cereus var. mycoides and characterization of its products. | the cloned gene was composed of 1638 bp for coding plus promoter like and sd-like sequences ahead of it. the deduced amino acid sequence had high similarity with known beta-amylases. the n-terminal sequence of the cloned beta-amylase seemed to be a signal peptide. the gene was introduced into bacillus subtilis 1a289 using phy300plk as a vector and the expressed protein was recovered from the culture media. the enzyme fraction produced was divided into two components upon the deae column chromato ... | 1996 | 8987540 |
| accumulation of benzoic acid in suspension cultured cells of pinus thunbergii parl. in response to phenylacetic acid administration. | the generation and accumulation of both benzoic acid (ba) and its conjugates were induced in suspension cultured cells of pinus thunbergii by administering either phenylacetic acid (pa), a toxic metabolite of bacillus cereus (strain hy-3) accompanying the pine wood nematode, or a lyophilized culture supernatant of this bacterium. ba conjugates reached their maximal levels in quantity two days after the administration and then decreased gradually until the 14th day, while ba increased significant ... | 1996 | 8987587 |
| two investigational glycylcyclines, dmg-dmdot and dmg-mino. antimicrobial activity studies against gram-positive species. | dmg-dmdot (cl-331,002 or cl-331,928) and dmg-mino (cl-329,998 or cl-344,677) are two new semisynthetic tetracyclines called glycylcyclines, with a broad spectrum of activity and includes enterobacteriaceae, gram-positive cocci, jk diphtheroids, and bacillus cereus. potent activity was demonstrated against all streptococcus spp. strains [minimum inhibitory concentrations] (mic90s) 0.06-0.25 micrograms/ml) and staphylococci (oxacillin susceptible ans resistant; mic90s 0.12-2 micrograms/ml). both g ... | 1996 | 8988765 |
| risk factors for outbreaks of infectious intestinal disease linked to domestic catering. | the epidemiology of general outbreaks of infectious intestinal disease associated with domestic catering for large numbers is described and compared with foodborne outbreaks in other settings. from 1 january 1992 to 31 december 1994, the phls communicable disease surveillance centre identified 101 foodborne general outbreaks of infectious intestinal disease associated with domestic catering in england and wales (16% of all foodborne outbreaks). salmonella species were associated with 77 of the 1 ... | 1996 | 8990573 |
| [site-specific bcuai endonuclease from bacillus cereus a]. | a new restriction endonuclease was isolated from the bacillus cereus bkm b-814 by means of the cell disruption with ultrasonication, ammonium sulfate fractionation of the cell-free extract, and chromatography on deae-sepharose to give about 1400 u of the enzyme per gram of cells. the enzyme revealed the maximum activity at 30-37 degrees c, ph 7.6-8.2, and 5-10 mm mgcl2 under a high ionic strength (50 mm tris-hcl, 100 mm nacl). the site-specific endonuclease bcuai was found to recognize the 5' g ... | 1996 | 8992958 |
| [exotoxins of bacillus cereus associated with food poisoning]. | 1996 | 8994344 | |
| tripartite hemolysin bl from bacillus cereus. hemolytic analysis of component interactions and a model for its characteristic paradoxical zone phenomenon. | hemolysin bl (hbl) is a unique membrane-lytic toxin from bacillus cereus composed of three distinct proteins, designated b, l1, and l2. hbl produces a paradoxical zone phenomenon in gel diffusion assays in sheep blood agar. lysis does not begin immediately adjacent to the source of diffusion; rather, it begins several millimeters away. cells near the source and at intersections of lysis zones remain intact longer. here, we developed a spectrophotometric hemolysis assay system that measures the a ... | 1997 | 8995253 |
| incidence of diarrhea with antibiotics and the increase of clostridia in rabbits. | rabbits were treated with a single intravenous injection of various antibiotics. more than 40 per cent of the animals showed diarrhea after being treated with sulbactam/cefoperazone, cefmetazole, clindamycin, piperacillin or aspoxicillin. clostridium difficile was isolated from sulbactam/cefoperazone-treated diarrheic rabbits, with their cecal contents showing positive reaction in a latex agglutination test for c. difficile enterotoxin. however, 27 cefmetazole-induced diarrheic cases were not as ... | 1996 | 8996700 |
| discrimination between bacillus cereus and bacillus thuringiensis using specific dna probes based on variable regions of 16s rrna. | identification of bacillus cereus and differentiation between b. cereus and closely related species are currently based on biochemical tests. the main problem is to discriminate between b. cereus and b. thuringiensis. sequencing part of the 16s rrna showed that several b. cereus isolates present in food and involved in food poisoning, confirmed according to the classical biochemical methods, were in fact b. thuringiensis. as this organism is the most commonly used microbial insecticide worldwide ... | 1997 | 8997705 |
| altering substrate specificity of bacillus sp. sam1606 alpha-glucosidase by comparative site-specific mutagenesis. | the bacillus sp. sam1606 alpha-glucosidase with a broad substrate specificity is the only known alpha-glucosidase that can hydrolyze alpha,alpha'-trehalose efficiently. the enzyme exhibits a very high sequence similarity to the oligo-1,6-glucosidases (o16g) of bacillus thermoglucosidasius and bacillus cereus which cannot act on trehalose. these three enzymes share 80% identical residues within the conserved regions (cr), which have been suggested to be located near or at the active site of the a ... | 1997 | 8999834 |
| haem o and a putative cytochrome bo in a mutant of bacillus cereus impaired in the synthesis of haem a. | in a spontaneous mutant (pym1) of bacillus cereus impaired in the synthesis of haem a, no haem-a-containing cytochromes were detected spectroscopically. the haem a deficiency was compensated by high levels of haem o and a co-reactive cytochrome o in membranes; no other oxidases were detected. in contrast, the wild-type strain had considerable amounts of haem a and negligible levels of haem o. the mutant pym1 exhibited normal colony morphology, growth, and sporulation in nonfermentable media, whe ... | 1997 | 9000338 |
| site-specific introduction of an electroactive label into a non-electroactive enzyme (beta-lactamase i). | a cysteine residue was introduced close to the active site of beta-lactamase i by site-directed mutagenesis to replace tyrosine-105 and was subsequently modified with an electroactive sh-specific reagent, n-(2-ferrocene-ethyl)maleimide. the resulting modified enzyme became electroactive, showing good quasireversible electrochemistry which was characteristic of the attached ferrocene moiety while retaining its specific enzymatic activity. in the presence of a suicide substrate, 6beta-iodopenicill ... | 1997 | 9001388 |
| fulminating bacteremia and pneumonia due to bacillus cereus. | we present two cases of rapidly progressing, fatal pneumonia caused by bacillus cereus. these cases are interesting in that b. cereus, even from blood or sputum specimens, may often be considered a contaminant and receive inadequate attention. also of interest was the fact that the two patients resided in the same area of the state, were welders by trade, and became ill within a few days of each other, yet there was no epidemiologic link between them. | 1997 | 9003628 |
| genotypic and phenotypic analysis of zwittermicin a-producing strains of bacillus cereus. | many strains of bacillus cereus produce zwittermicin a, a novel antibiotic that contributes to the ability of b. cereus to suppress certain plant diseases. the purpose of this study was to identify molecular indicators of zwittermicin a production in b, cereus strains, contribute to an understanding of the ecology and evolution of this group of bacteria, and identify potential agents for control of plant disease. the fatty acid composition of 20 strains known to be zwittermicin a producers and 2 ... | 1996 | 9004505 |
| response of three microbial test systems to pesticides. | 1997 | 9008061 | |
| [the detection of toxinogenic bacillus cereus strains]. | investigations into optimal culture conditions for bacillus cereus in order to detect bacterial toxins in a cell culture system showed that dulbecco's modified eagle's medium supplemented with 5% fetal calf serum is the medium best suited for this purpose. the highest toxicity levels were seen when bacteria were cultured at 21 degrees c. in 42 of 43 bacillus cereus-strains isolated from milk and milk products, toxin formation was detected using the mtt-test. in 11 strains, toxin formation was ev ... | 1996 | 9012242 |
| rhabdomyolysis associated with septicemia after autologous bone marrow transplantation. | 1997 | 9012940 | |
| kinetics of phosphatidylinositol-specific phospholipase c with vesicles of a thiophosphate analogue of phosphatidylinositol. | 1,2-dimyristoyloxypropane-3-thiophospho(1d-1-myo-inositol) (d-thio-dmpi) was synthesized as a substrate for the continuous spectrophotometric assay of phosphatidylinositol-specific phospholipase c (pi-plc) from bacillus cereus. release of thio-diglyceride is followed by a coupled reaction with 4,4'-dithiopyridine to produce a chromophore, 4-thiopyridine, measured by its absorption at 324 nm. sonicated vesicles of d-thio-dmpi gave sigmoidal michaelis-menten kinetics with pi-plc as a function of b ... | 1996 | 9022217 |
| monoclonal antibodies for use in detection of bacillus and clostridium spores. | five monoclonal antibodies against bacterial spores of bacillus cereus t and clostridium sporogenes pa3679 were developed. two antibodies (b48 and b183) were selected for their reactivity with b. cereus t spores, two (c33 and c225) were selected for their reactivity with c. sporogenes spores, and one (d89) was selected for its reactivity with both b. cereus and c sporogenes spores. the isotypes of the antibodies were determined to be immunoglobulin g2a (igg2a) (b48), igg1 (b183), and igm (c33, c ... | 1997 | 9023926 |
| a bacillus cereus member of the snf2 family. | the complete sequence of a bacillus cereus member of the snf2 family of putative helicases showed conservation of all seven motifs typical of this family. bcsnf predicted a protein of 1064 aa where the conserved snf2 domain was located at the carboxy terminus, whereas the 633 amino-terminal aa showed no homology to any protein in the databases. a putative transcriptional start was identified by primer extension, indicating that bcsnf is not a part of a larger operon. no phenotypical changes were ... | 1997 | 9025290 |