Publications

[study on the infections of u. urealyticum, m. hominis, and c. trachomatis in patients with venereal diseases and healthy controls in three areas of china].	two hundred and thiry-nine patients with venereal diseases and 264 healthy controls, from yichang, liuzhou, and beijing, were detected for the antibodies to ureaplasma urealyticum (uu). mycoplasma hominis (mh), and chlamydia trachomatis (ct) by indirect hemagglutination (iha) in 1990. the results showed that: 1) in patients with venereal diseases, the positive rates was the highest in ct (27.62%), higher in uu (17.57%), and lower in mh (4.18%); 2) the levels of antibody to ct and uu were signifi ...	1992	1301266
[long lasting conjunctivitis: research of etiological factors].	forty children with long-lasting or recurrent conjunctivitis were included in this etiological study. it is well known that purulent conjunctivitis is mainly bacterial, with a major source of infection from chlamydia; recently, however, a greater percentage of viral forms, with the exception of conjunctivitis without secretion, has been reported. the authors focused their attention on the clinical symptoms and on bacteriological studies of the forms of chlamydia and mycoplasma conjunctivitis, hi ...	1992	1301484
[prevalence in tucumán of chlamydia trachomatis and ureaplasma urealyticum in sexually active women].	a group of 298 sexually active women who consulted for cervicitis, sterility or infertility were studied to detect the prevalence of chlamydia trachomatis and ureaplasma urealyticum and also to correlate the presence of these pathogens with tube damage. chlamydial antigens were detected with a fluorescent commercial reagent with specific monoclonal antibodies. the isolation of u. urealyticum was made following the shepard and lunceford (19) techniques. in all samples of endocervical canal, we ob ...	1992	1302867
high incidence of asymptomatic urogenital infection in patients with uveitis anterior.	acute anterior uveitis (aau) may be associated with systemic infectious or inflammatory disease. we examined 92 patients with the first attack of acute anterior uveitis; all patients were free of any extraocular symptoms. a thorough clinical examination did not reveal any systemic underlying disease. in the course of microbiological examination, however, a high incidence of asymptomatic infection of the urethra and/or cervix with ureaplasma urealyticum, chlamydia trachomatis and mycoplasma homin ...	1992	1303857
[threats of infectious factors in the uterine neck regarding chlamydia trachomatis in imminent premature deliveries].	the research concerned threats of infections factors to a endocervix in imminent premature deliveries. it was ascertained that chlamydia (c) trachomatis is the most frequent pathogenic factor, which occurred in 41.8% of cases. the premature delivery occurred in 16.3% of women with positive c. trachomatis test results.	1992	1303914
antiphospholipid antibodies and shrinking lungs in sle.		1997	1304411
in situ hybridisation: principles and applications.	in situ hybridisation (ish) is based on the complementary pairing of labelled dna or rna probes with normal or abnormal nucleic acid sequences in intact chromosomes, cells or tissue sections. compared with other molecular biology techniques applicable to anatomical pathology, ish enjoys better rapport with histopathologists because of its similarity to immunohistochemistry. it has the unique advantage over other molecular biology techniques--largely based on probe hybridisation with nucleic acid ...	1992	1304627
[infection with chlamydia trachomatis among women with infertility treatment].	68 women with sterility treatment were tested. among 13 of them (20%) presence of chlamydial antibodies was found. hormonal profile was estimated. in a group of women with positive serologic reaction increased concentration of prolactin was found.	1992	1305128
pericytes in human breast stroma and the cells to which they relate.	the function of pericytes, the cells nearest the microvascular endothelium, has long been debated. on the basis of ultrastructural studies it is pointed out that they have specialised features in common with endothelial cells of the lymphatic labyrinth of the human breast. the latter are in continuity with the initial lymphatics. these features, which include points of stromal attachment, allow contraction or relaxation of the cell-processes. the cytoplasmic processes of the pericyte, when relax ...	1992	1305829
the efficacy of clarythromycin (a-56268, te-031) in the treatment of genital chlamydial infection.	the authors report the preliminary results of an experience of treatment with clarythromycin in chlamydia trachomatis endocervicitis/endourethritis; 100% of 51 outpatients treated had negative elisa results 7-10 days after the end of treatment with clarythromycin 500 mg. b.i.d. for 7 days. among 64 female outpatients with chlamydial cervicitis treated with erythromycin 1 g b.i.d. for 7 days, 88% were negative at elisa at the same intervals after therapy. the authors conclude that the efficacy of ...	1992	1306155
[home oxygen therapy using liquid oxygen system].	home oxygen therapy (hot) for patients with chronic respiratory failure has been believed to increase the quality of life and to improve the prognosis of such patients. in japan, hot for chronic respiratory failure has been supported by health insurance since march 1985. since then, patients with hot have increased progressively, and it has been reported that there are now over 20,000 patients with hot. the oxygen concentrator which extracts oxygen from atmospheric air has been commonly used as ...	1985	1306220
expression and release of neuroregulators during development: monoamines and neuropeptides.	the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ...	1992	1306802
biology and pharmacology of hematopoietic growth factors.	several glycoproteins that control blood formation have recently been characterized. through their overlapping, synergizing, and antagonistic effects, they regulate hematopoiesis in a highly differentiated network. large scale production of these colony stimulating factors (csfs) has been made available by recombinant dna technology, and a series of clinical studies in a variety of indications has been finished. in general, the subcutaneous application seems to be superior to the intravenous inj ...	1986	1306882
[preoperative evaluation of respiratory function].		1986	1306934
[tubo-ovarian transplantation in the treatment of sterility. experimental research].	microsurgical transposition of fallopian tube and ovary has the potential of being an efficient therapeutic treatment in patients with tubal sterility. the authors present their experience of microsurgical adnexal transplantation in rabbit by two different techniques: the first procedure by microvascular anastomosis of the ovarian vessels, the second one without vascular pedicle. function is evaluated at various time after grafting by: exploratory laparotomy on day 30 to establish whether circul ...	1992	1307024
[hepatic hydatidosis with glomerular involvement: report of a case].	a patient with hydatid cyst of the presented stunted growth, normal renal function and no significant proteinuria. the radiological abdominal investigation disclosed, besides the hydatid cyst, horseshoe kidneys. renal biopsy was performed during surgery. immunofluorescence (direct and indirect) and immunoperoxidase studies revealed glomerular deposits of hydatid antigen and its corresponding antibody. a grade i immune complex type of membranous glomerulonephritis, without significant clinical si ...	2005	1307057
[epidemiology of bacteremias at a university hospital].	the epidemiological aspects of bacteremias were determined in a university hospital, according to service, age, sex and place of acquisition (nosocomial or community-acquired). from august 1985 through july 1986 the incidence rate of bacteremias at the sao paulo hospital was 21.7/1000 admissions. such a high rate is probably related to the characteristics of the patients admitted to the hospital. the incidence was higher in the extremes of age and was similar in either sex. approximately 60% of ...	2005	1307068
diagnosis of the sinus venosus defect by echocardiography and angiocardiography.	a retrospective analysis of forty five cases of sinus venosus defect was done to compare the ability of transthoracic echocardiography, transesophageal echocardiography and cineangiography in diagnosing this lesion. left atrial and superior vena cava angiography delineated sinus venosus defect accurately in all the 45 cases (100%). transthoracic cross-sectional echocardiography combined with colour flow mapping could visualize this defect in 28 out of 45 cases (62.2%) and proved inferior to tran ...	2005	1307089
the effects of 21 or 30% o2 plus umbilical cord occlusion on fetal breathing and behavior.	we have shown previously that continuous fetal breathing can be induced by 100% o2 alone or combined with umbilical cord occlusion (baier, hasan, cates, hooper, nowaczyk & rigatto, 1990). to know whether it could also be induced by lower o2 concentrations plus cord occlusion, we studied 9 chronically instrumented fetal sheep (16 experiments) using our window model. after a baseline cycle [1 low voltage + 1 high voltage electrocortical activity (ecog) epoch] the fetal lung was distended via an en ...	1992	1307099
inhibitory role of gamma-amino-butyric acid in the rabbit urinary bladder.	gamma-amino-butyric acid (gaba) is an established inhibitory neurotransmitter in the central nervous system (cns) and it has also been identified in the bladder. we have investigated in the rabbit the effect of gaba on detrusor activity. rabbit detrusor muscle strips were made to contract by electrical stimulation of their autonomic nerves or by the addition of carbachol. the addition of gaba caused substantial inhibition of muscle contraction. gaba acts on 2 classes of receptors-gabaa and gabab ...	1992	1310630
genital papillomavirus infection in women treated for chlamydial infection.	samples for chlamydia testing were taken from 298 and pap smear from 284 non-pregnant sexually active young women in a midwife-run family planning dervice. chlamydia was found in 36 (12.1%). three women (1.1%) had cytological atypia corresponding to cin i or ii. koilocytosis was seen in 9 smears (2.8%). thirty-two of the chlamydia-positive women were followed for a mean of 15 months by a gynaecologist with chlamydia tests, colposcopy, pap-smears and in some cases biopsies. there were 7 reinfecti ...	1992	1311961
the 'other' sexually transmitted diseases. chlamydial, herpes simplex virus, and human papillomavirus infections.	the incidence of sexually transmitted diseases continues to rise across the country. because many cases are subclinical and asymptomatic, chlamydial, herpes simplex virus, and human papillomavirus (hpv) infections continue to spread at an alarming rate. these infections can lead to serious sequelae, such as pelvic inflammatory disease, infertility, and cervical dysplasia or carcinoma, so improved disease-control strategies are needed. preventive efforts should include use of the latest diagnosti ...	1992	1312236
clarithromycin and azithromycin: new macrolide antibiotics.	the chemistry, mechanism of action, antimicrobial spectrum, pharmacokinetics, clinical efficacy, adverse effects, drug interactions, and dosage and administration of clarithromycin and azithromycin are described. clarithromycin and azithromycin are new macrolide antibiotics that are similar in structure to erythromycin. compared with erythromycin, clarithromycin demonstrates increased activity against staphylococcus aureus, streptococci, legionella pneumophila, moraxella catarrhalis, and chlamyd ...	1992	1312921
isoform-specific regulation of platelet-derived growth factor activity and binding in osteoblast-enriched cultures from fetal rat bone.	in osteoblast-enriched cultures from fetal rat bone, the a-chain homodimer of platelet-derived growth factor (pdgf-aa) is less potent than the pdgf isoforms containing b chain subunits (pdgf-ab and pdgf-bb), but normal osteoblasts appear to synthesize only pdgf-a subunit mrna and polypeptide. however, other agents may regulate pdgf-aa activity in skeletal tissue. pretreatment of osteoblast-enriched cultures with interleukin 1 alpha (il-1 alpha) or tumor necrosis factor-alpha (tnf-alpha) synergis ...	1992	1313443
inhibition of ca2+ and k+ currents by "antifreeze" proteins.	for the last two decades, the research on fish "antifreeze" proteins has focused exclusively on their ability to depress noncolligatively blood plasma freezing points, presumably by binding to ice crystals. we report evidence that antifreeze polypeptides from the winter flounder (pseudopleuronectes americanus) have another special property, the ability to block ion channels. in experiments with porcine granulosa cells we show, using the patch-clamp technique in the whole cell configuration, that ...	1992	1313654
monoclonal antibodies for the identification of herpesvirus simiae (b virus).	to differentiate between b virus and hsv isolates from monkeys and man monoclonal antibodies (mabs) were produced to herpesvirus simiae (b virus) and herpes simplex type 1 and 2 (hsv-1 and hsv-2). mabs were tested by indirect immunofluorescence (ifat) for reactivity against herpesviruses from asiatic monkeys (b virus), african monkeys (sa 8 virus), and man (hsv-1, hsv-2, varicella-zoster virus, cytomegalovirus, and epstein-barr virus). mabs could be divided into groups a-e displaying specific re ...	1992	1314049
hepatocellular carcinoma in africans.	hepatocellular carcinoma is endemic in africa, where in the incidence of the disease in males ranges from 20-100,000 per annum. the tumor tends to occur at a younger age compared to the age of presentation in europeans or chinese. the majority of african patients with hcc are hbsag positive, but hbsag is more commonly detected in younger vs older patients. approximately 30% of patients are anti-hcv positive. both these chronic virus infections may induce disease via the development of cirrhosis. ...	1992	1314116
bronchioalveolar carcinoma in a 15-year-old girl.	this report describes a 15-year-old female from venezuela who has bronchioalveolar carcinoma. she is one of the youngest patients with this particular diagnosis reported in the literature. the postulated age of onset in this case was 13 years, when the first lesion was noted. bronchioalveolar carcinoma is an extremely rare diagnosis in the pediatric population. the paucity of symptoms in this disease can lead to a delay in diagnosis. malignancy should be considered in all cases of pediatric pulm ...	2001	1314155
[regressive diffuse leukoencephalitis under foscarnet in an aids infected patient].		1992	1314379
the gamma 1(34.5) gene of herpes simplex virus 1 precludes neuroblastoma cells from triggering total shutoff of protein synthesis characteristic of programed cell death in neuronal cells.	the gamma 1(34.5) gene of herpes simplex virus 1 was previously shown to play a role in viral virulence since deletion of the gene reduced by a factor of approximately 100,000 the capacity of the virus to replicate in the central nervous system and cause mortality in the mouse. here we show that in the human neuroblastoma cell line sk-n-sh of neuronal origin gamma 1(34.5) null mutants expressed early proteins, viral dna, and mrna of late genes. however, the onset of viral dna synthesis triggered ...	1992	1314384
gamma-aminobutyric acid a or c receptor? gamma-aminobutyric acid rho 1 receptor rna induces bicuculline-, barbiturate-, and benzodiazepine-insensitive gamma-aminobutyric acid responses in xenopus oocytes.	xenopus oocyte expression of the recently cloned gamma-aminobutyric acid (gaba) rho 1 receptor subunit cdna yields a pharmacologic profile characteristic of the gabac responses described by johnston [benzodiazepine/gaba receptors and chloride channels. receptor biochemistry and methodology (r. w. olsen and j. c. venter, eds), vol. 5. alan r. liss, new york, 57-71 (1986)] and the responses to retinal mrna recently reported in the xenopus expression system [proc. natl. acad. sci. usa 88:4318-4322 ...	2005	1314944
characteristics of neisseria gonorrhoeae isolated in australia showing decreased sensitivity to quinolone antibiotics.	forty three strains of neisseria gonorrhoeae with decreased sensitivity to quinolone antibiotics were detected amongst 2141 australian isolates of gonococci examined in the years 1984 to 1990. the strains examined belonged to 23 different auxotype/serovar classes, were generally more resistant to other antibiotics and, in the majority of cases, were isolated from travellers entering or returning to australia from se asia. quinolone-sensitive wild-type gonococci became less sensitive to these age ...	1992	1315946
cis-regulation of the l-type pyruvate kinase gene promoter by glucose, insulin and cyclic amp.	the glucose/insulin response element of the l-pyruvate kinase gene is a perfect palindrome located from nt -168 to -144 with respect to the cap site. this element (l4) is partially homologous to mltf binding sites. its full efficiency requires cooperation with a contiguous binding site for hnf4, termed l3 and located from nt -145 to -125. in the presence of the l4 element contiguous to l3, cyclic amp inhibits activity of the l-pk promoter while in its absence, or when the normal l4-l3 contiguity ...	1992	1315961
comparative evaluation of clindamycin/gentamicin and cefoxitin/doxycycline for treatment of pelvic inflammatory disease: a multi-center trial. the european study group.	the clinical efficacy and safety of clindamycin-gentamicin versus doxycycline-cefoxitin in the treatment of acute pelvic inflammatory disease was evaluated in a comparative, randomized, prospective, multicenter study. ten investigators enrolled 170 patients. those judged to be eligible for efficacy were 60/88 (68%) who received the clindamycin-gentamicin regimen and 55/82 (67%) of those treated with cefoxitin-doxycycline. a successful clinical outcome was attained for 52/60 (87%) and 46/55 (84%) ...	1992	1316041
presence of the diffuse early antigen of epstein-barr virus in lymphomas and lymphoproliferative disorders.	the authors recently demonstrated that 40% of epstein-barr virus (ebv) associated lymphoproliferative lesions contained lytic as well as latent ebv dna. to examine more fully the replicative state of ebv in these disorders, the authors studied protein extracts of ebv-associated lymphoid lesions from 13 patients, most of whom were immunosuppressed, for expression of the diffuse early antigen (ea-d) of ebv, by immunoblotting techniques. the reagent used was a mouse monoclonal antibody. seven of th ...	1992	1316087
ultrasound findings in perihepatitis associated with pelvic inflammatory disease.	the width of the right anterior extrarenal tissue was increased on ultrasound examination in 9 patients with perihepatitis (fitz-hugh-curtis syndrome). normal values were obtained in 72 women without detectable or known disease and were found to be between 1 mm and 5 mm (mean 1.8 mm). the 9 patients with laparoscopically verified perihepatitis who demonstrated this thickening had values ranging from 6 mm to 12 mm, (mean 7.7 mm). although the incidence of this inflammatory thickening in perihepat ...	1992	1316376
leukotriene b4 omega-oxidation by human polymorphonuclear leukocytes is inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa.	human polymorphonuclear leukocytes (pmnl) metabolize the potent chemotaxin leukotriene b4 (ltb4) by omega-oxidation to 20-hydroxyl-ltb4 and 20-carboxy-ltb4. the ability of unstimulated human pmnl to metabolize exogenous ltb4 was found to be inhibited by pyocyanin, a phenazine derivative produced by pseudomonas aeruginosa, in a dose-dependent manner. 1-hydroxyphenazine (1-ohp), a metabolite of pyocyanin, was not inhibitory under identical conditions. the initial enzymic step in the conversion of ...	1992	1316878
updates on chlamydia.		1992	1317033
in vitro and in vivo activities of sparfloxacin, other quinolones, and tetracyclines against chlamydia trachomatis.	sparfloxacin was more potent than other quinolones (tosufloxacin, lomefloxacin, ciprofloxacin, ofloxacin, fleroxacin, enoxacin, and norfloxacin) and as potent as minocycline and doxycycline in activity against chlamydia trachomatis in vitro and in vivo. sparfloxacin was more bactericidal than minocycline against c. trachomatis d/uw-3/cx.	1992	1317145
ligand-induced homo- and hetero-dimerization of platelet-derived growth factor alpha- and beta-receptors in intact cells.	porcine aortic endothelial cells expressing platelet-derived growth factor (pdgf) alpha- or beta-receptors after transfection of the corresponding cdnas, were used to investigate whether pdgf receptor dimerization occurs in intact cells after ligand binding. using three different methods--covalent cross-linking of 125i-labeled ligand, cross-linking of metabolically labeled cells after ligand-binding followed by immunoprecipitation, and immunoblotting of cells after ligand binding and cross-linki ...	1992	1317195
effect of captopril on the release of the components of the renin-angiotensin system into plasma and lymph.	the effects of captopril on the intrarenal renin-angiotensin system were assessed from measurements in arterial plasma, renal venous plasma, and renal lymph from salt-depleted dogs. in the basal state, immunoreactive angiotensin ii (ang ii) in renal venous plasma averaged only 60 +/- 12% (p less than 0.01) of arterial plasma, although the concentration of ang ii in renal lymph was 2.0 +/- 0.4-fold (p less than 0.05) greater. the ang ii concentration of renal lymph incubated ex vivo at 37 degrees ...	1992	1317225
regulation of pregnenolone synthesis in c6-2b glioma cells by 4'-chlorodiazepam.	an experimental model to study synthesis of cholesterol and pregnenolone from the precursor mevalonolactone (mva) was developed in c6-2b glioma cells. the steroidogenic capability of this cell line and the regulation of pregnenolone production by 4'-chlorodiazepam (4'cd), a specific ligand for the mitochondrial diazepam binding inhibitor (dbi) receptor (mdr), were investigated. cells maintained in serum-free media were incubated with lovastatin (20 microm) and two inhibitors of pregnenolone meta ...	1992	1317582
solvent dielectric effects on protein dynamics.	electron paramagnetic resonance (epr) spectroscopy and molecular dynamics (md) simulations were used to investigate the dynamics of alpha-chymotrypsin in solvents ranging in dielectric constant from 72 to 1.9. epr measurements showed that motions in the vicinity of two spin-labeled amino acids (met-192 and ser-195) decreased dramatically with decreasing solvent dielectric constant, a trend consistent with changes in the electrostatic force between charged residues of the protein. epr results and ...	2006	1317584
development of a monoclonal antibody-based p24 capsid antigen detection assay for htlv-i, htlv-ii, and stlv-i infection.	a monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay (elisa) was developed and employed to detect p24 capsid antigen from human t-cell lymphotropic viruses type i and ii (htlv-i, htlv-ii), simian t-cell lymphotropic virus type i (stlv-i)-infected cell lines, and from mononuclear cell cocultures of htlv-infected humans and stlv-i infected monkeys. a monoclonal antibody specific for htlv p24 and p53 capsid antigens was coated onto 96-well microtiter plates to capture htlv/ ...	1992	1318063
sequential infection of glial cells by the murine hepatitis virus jhm strain (mhv-4) leads to a characteristic distribution of demyelination.	an antigenic variant of the neurotropic murine coronavirus jhmv, designated 2.2-v-1, causes marked demyelination in the relative absence of encephalitis. it is thus useful for the study of the pathogenesis of demyelinating lesions. to better understand the sequential events leading to demyelination, we have examined murine brain and spinal cord tissue at daily intervals after intracerebral inoculation, evaluating them for the distribution of viral antigen, leukocyte infiltration, and demyelinati ...	1992	1318460
in vitro evaluation of activities of azithromycin, erythromycin, and tetracycline against chlamydia trachomatis and chlamydia pneumoniae.	the in vitro activities of azithromycin (cp-62,993; pfizer), erythromycin, and tetracycline were evaluated by inhibiting chlamydia trachomatis and chlamydia pneumoniae, formerly twar, propagation in vitro in mccoy cells, hela cells, and hl cells. eleven clinical isolates of c. trachomatis (serovars d, e, f, j, k, and l2) and four strains of c. pneumoniae were tested with an inoculum of 10(3) inclusion-forming units in a 96-well microtiter plate. the mic ranges of these antimicrobial agents again ...	1992	1318677
new monoclonal antibodies for the detection of immediate early antigens of cytomegalovirus.	two new monoclonal antibodies, cie-1 and cie-2, were developed for the rapid detection of human cytomegalovirus (hcmv) infection. they were found to be reactive with immediate early protein of hcmv in the nuclei of infected fibroblasts, as early as 3 hours post-infection. by radioimmunoprecipitation, cie-1 was found to react with a protein with an apparent molecular weight of 70,000, whereas cie-2 precipitated 2 proteins of 70,000 and 72,000 daltons, respectively. both monoclonal antibodies reco ...	1992	1319172
infections of the cervix uteri with human papillomavirus and chlamydia trachomatis.	apart from infection with human papillomavirus (hpv), other microorganisms may be involved in the development of cervical neoplasia. to study concomitant infections with hpv and chlamydia trachomatis, cervical specimens from 4 groups of women were examined for the presence of these microorganisms by the polymerase chain reaction. the first group consisted of 143 consecutive samples from women with no cytological abnormalities who participated in a triennial screening program to prevent cervical ...	1992	1320098
alpha 2-adrenoceptor density in forebrain areas of zucker rats: no effect of genetic obesity or antiglucocorticoid treatment.	the density of alpha 2-adrenoceptors was determined in different forebrain regions of lean and obese zucker rats by para-[125i]iodoclonidine ([125i]pic) receptor autoradiography. no significant differences were observed between the zucker phenotypes in the density of alpha 2-adrenoceptors in any brain areas examined. four days treatment with the antiglucocorticoid mifepristone (ru 38486) did not affect [125i]pic-binding. it is concluded that the hyperphagia and abnormal corticosterone secretion ...	1992	1322226
the in-vitro activity of two new quinolones: rufloxacin and mf 961.	the in-vitro activity of two new quinolone antimicrobials, rufloxacin and mf 961, together with the desmethylated metabolite of rufloxacin (mf 922) were compared with other orally administered agents against 622 bacterial strains. against enterobacteriaceae and pseudomonas aeruginosa rufloxacin was generally active (mic90 1-8 mg/l) with the exception of klebsiella and serratia spp. (mic90 32 mg/l and enterobacter spp. (mic90) 64 mg/l. the respiratory pathogens haemophilus influenzae and moraxell ...	1992	1324239
in vitro activity of nonoxynol 9 on hela 229 cells and primary monkey cervical epithelial cells infected with chlamydia trachomatis.	nonoxynol 9 (non-9) is the active ingredient in a wide variety of vaginal contraceptive preparations. the manufacturer recommendation for optimal contraceptive practice is repeated application every 6 h. we studied the in vitro activity of non-9 against chlamydia trachomatis (e/uw-5/cx) and its toxicity against hela 229 cells and monkey cervical epithelial cells. with a contact time of 6 h, non-9 was toxic to hela cells at concentrations of 50 micrograms/ml or greater and to monkey cervical cell ...	1992	1324646
chlorination of cellulose with n-chlorosuccinimide-triphenylphosphine under homogeneous conditions in lithium chloride-n,n-dimethylacetamide.	microcrystalline cellulose was chlorinated with n-chlorosuccinimide-triphenylphosphine under homogeneous conditions in licl-n,n-dimethylacetamide. at the early stage of the reaction only replacement of the 6-hydroxyl groups with chlorine was observed, and 3-hydroxyl groups were replaced at a lower rate with walden inversion. the effects of reaction conditions on the extent of chlorination were studied in detail. more than two equivalents of chlorination reagents per glucose residue were necessar ...	2004	1324798
effects of il-4, il-5, and il-6 on growth and immunoglobulin production of epstein-barr virus-infected human b cells.	in the present study we investigated whether interleukin-4 (il-4), il-5, and il-6 could enhance the efficiency of epstein-barr virus (ebv) transformation for the generation of specific human monoclonal antibody (humab)-producing b-cell lines directed against erythrocyte rhesus(d) antigen. in newly ebv-infected b cells, il-4 and il-6 caused a comparable enhancement of proliferation and of total igg and iga production. il-6 showed a much stronger effect than il-4 on igm production, whereas il-4 wa ...	1992	1324802
generalized non-convulsive epilepsy: focus on gaba-b receptors.		2005	1324976
generalized non-convulsive epilepsy: focus on gaba-b receptors.		2005	1324976
a controlled trial of a single dose of azithromycin for the treatment of chlamydial urethritis and cervicitis. the azithromycin for chlamydial infections study group.	currently, there is no single-dose therapy that is effective in the treatment of urethral or endocervical infections with chlamydia trachomatis. azithromycin is a new azalide antibiotic that has substantial activity against c. trachomatis, is concentrated intracellularly, and has a long half-life in serum and tissue.	1992	1325036
the liver as a stem cell and lineage system.	we propose that the liver is a stem cell and lineage system with many parallels to lineages in the bone marrow, gut, and epidermis, varying from them only in kinetics. all are organized with three compartments: a slow cycling stem cell compartment with cells expressing a fetal phenotype and responding slowly to injury; an amplification compartment with cells of intermediate phenotype rapidly proliferating in response to regenerative stimuli or acute injuries; and a terminal differentiation compa ...	2006	1325126
transfer of tn916 between lactococcus lactis subsp. lactis strains is nontranspositional: evidence for a chromosomal fertility function in strain mg1363.	lactococcus lactis subsp. lactis mg1363 can act as a conjugative donor of chromosomal markers. this requires a chromosomally located fertility function that we designate the lactococcal fertility factor (laff). using inter- and intrastrain crosses, we identified other l. lactis strains (lmo230 and mms373) that appear to lack laff. the selectable marker in our crosses was tcr, carried by tn916, a transposon present on the chromosome. the transfer of tcr was not due to tn916-encoded conjugative fu ...	1992	1325966
herpesvirus 6 infection in young children.		1992	1326080
clinical course of cervical human papillomavirus lesions in relation to coexistent cervical infections.	a prospective follow-up of 530 women with cervical human papillomavirus (hpv) infection was conducted from 1981 to the present (mean 62.9 months). the patients were examined by pap smears and colposcopy with or without biopsies every sixth month. endocervical swabs were taken for culture of cytomegalovirus (cmv), herpes simplex virus (hsv), and chlamydia trachomatis at each visit. during the follow-up period, 179 of the 530 patients (33.8%) had cervical infection and 351 (66.2%) had no coexisten ...	1992	1326128
survey of the treatment of chlamydia trachomatis infection of the female genital tract.	a questionnaire was sent to every tenth (n = 302) general practitioner in norway. the physicians were to indicate their choice of antibiotic regimen for the treatment of genital chlamydial infections in women. sixty-nine percent of the practitioners responded. the choice of treatment varied widely between the respondents. forty-two different regimens were used for chlamydial cervicitis, 34 for cervicitis in pregnancy and 63 for probable pelvic infection. of the prescribed treatments for these th ...	1992	1326211
[two cases of esophageal varices complication after hepatic arterial infusion chemotherapy (hai) for metastatic liver tumor].	mild liver dysfunction is a well-known complication of hai, but it has been thought to be transient and reversible in most cases. in the case, of metastatic liver disease, in particular, hai has been performed safely because liver function is normal for the most part. we encountered 2 cases of irreversible severe liver dysfunction and esophageal varices after hepatectomy for metastatic liver tumor from colorectal cancer. they were treated with postoperative adjuvant hai. biliary enzyme as alkali ...	2006	1326919
[nephroblastoma and fragile x syndrome].		2006	1326936
[genital infections with chlamydia trachomatis in pregnant women in new caledonia].	chlamydia trachomatis was researched from 400 pregnant women. 76 of them were found infected and 129 carried serological signs of infection. we purpose a detection at the end of pregnancy interesting women with sexually transmitted diseases background and those with serological positive results (greater than 1/64). then, treatment of the mother will be an useful method of prevention against neonatal infection.	1992	1327347
[seroepidemiologic study of sexually transmitted diseases caused by chlamydia trachomatis in casablanca (morocco)].	screening 579 sera specimens obtained in casablanca (morocco) we have performed a retrospective seroepidemiological survey with regard to the prevalence of chlamydia trachomatis involved in genital tract infections. the surveyed populations are divided into 3 groups: 177 patients affected with sexually transmitted diseases (std), 319 reference patients, 83 maghrebian patients affected with std but living in france. the study was performed by the indirect microimmunofluorescence technique. the pr ...	1992	1327348
prevalence and cytologic manifestations of human papilloma virus (hpv) types 6, 11, 16, 18, 31, 33, 35, 42, 43, 44, 45, 51, 52, and 56 among 500 consecutive women.	the prevalence and associated cytologic manifestations of cervical infection with human papillomavirus (hpv) types 6, 11, 16, 18, 31, 33, 35, 42, 43, 44, 45, 51, 52, and 56 were studied among 500 consecutive women attending the harborview medical center sexually transmitted diseases (std) clinic in seattle, wa. using radiolabeled-probes without prior amplification of dna, hpv dna was detected in cervical specimens from 120 (24%) of the women and was found to be more prevalent than chlamydia trac ...	1992	1328077
a cohort study of the risk of cervical intraepithelial neoplasia grade 2 or 3 in relation to papillomavirus infection.	human papillomavirus (hpv) has been associated with cervical intraepithelial neoplasia, but the temporal relation between the infection and the neoplasia remains unclear, as does the relative importance of the specific type of hpv, other sexually transmitted diseases, and other risk factors.	1992	1328880
[prevalence of chlamydia trachomatis in genital samples from abidjan].	a study of direct genital swabs achieved in abidjan, on 116 men and 131 women consulting for urogenital complaints, has revealed that the men show a prevalence of 28.4% chlamydia trachomatis, and of 18.1% neisseria gonorrhoeae. concerning the women the prevalence of the same germs are 13.7% for chlamydia trachomatis, and 4.6% for neisseria gonorrhoeae. these results show the importance of chlamydia trachomatis as a sexually transmitted disease in abidjan (côte-d'ivoire). no differences were obse ...	1992	1330137
efficacy of single-dose azithromycin versus doxycycline in the treatment of cervical infections caused by chlamydia trachomatis.	the efficacy of single-dose azithromycin therapy in the treatment of cervical chlamydia trachomatis infections was compared to that of a standard seven-day course of treatment with doxycycline. cervical samples from 60 patients reacted positively in an enzyme immunoassay for detection of chlamydia trachomatis. in 31 patients chlamydia trachomatis was isolated from the sample taken before treatment. fourteen of the 31 patients were treated with doxycycline and 17 with azithromycin. all cultures o ...	1992	1330565
reversibility of heat shock in chlamydia trachomatis.	the heat shock effect on chlamydia development was studied. we report here that the reversibility of the heat shock response did not depend on the stage of chlamydial morphogenesis at which transfer to high temperature occurred, and the infectivity of the particles produced was not affected significantly, so long as the heat shock exposure was not prolonged. exposure to heat shock for more than 9 h resulted in stagnation of the growth cycle, appearance of aberrant reticulate body particles and l ...	1992	1330821
a double blind study of single dose azithromycin and doxycycline in the treatment of chlamydial urethritis in males.	to compare the efficacy and safety of azithromycin and doxycycline in the treatment of males with uncomplicated urethritis caused by chlamydia trachomatis.	1992	1330880
the use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis.	ocular swabs from 30 consecutive patients with follicular conjunctivitis were screened for chlamydia trachomatis, herpes simplex virus, and adenovirus by a variety of laboratory techniques. for the detection of c. trachomatis, we compared two polymerase chain reaction methods, mccoy cell culture isolation, and the direct fluorescein-conjugated monoclonal antibody test. four of 30 patients tested positive for c. trachomatis by using both conventional and biotinylated polymerase chain reaction met ...	1992	1334375
a molecular view of adipose tissue.		1991	1335984
cytologic evidence of the association of different infective lesions with dysplastic changes in the uterine cervix.	cervical smears from 4055 women were examined and classified as per who criteria. 873 (21.53%) smears revealed dysplastic changes. the dysplastic smears were further examined cytologically for the presence of chlamydia trachomatis, herpes simplex virus type-2, human papilloma virus, trichomonas vaginalis and candida albicans. chlamydia was found to be the commonest micro-organism associated with cervical dysplasia followed by herpes simplex virus type-2 and human papilloma virus. the association ...	1992	1336729
in vitro activity of sparfloxacin and other antimicrobial agents against genital pathogens.	the in vitro activity of sparfloxacin was determined for 60 strains of neisseria gonorrhoeae, 15 strains of chlamydia trachomatis and 40 strains each of gardnerella vaginalis, mycoplasma hominis, and ureaplasma urealyticum and compared with those of ampicillin, azithromycin, clarithromycin, erythromycin, ofloxacin, temafloxacin and tetracycline. sparfloxacin was active against all the strains studied and appeared to be the most potent quinolone tested. sparfloxacin had the lowest mics against n. ...	1992	1337506
purification, characterization and cdna cloning of human lung surfactant protein d.	human pulmonary surfactant protein d (sp-d) was identified in lung lavage by its similarity to rat sp-d in both its molecular mass and its ca(2+)-dependent-binding affinity for maltose [persson, chang & crouch (1990) j. biol. chem. 265, 5755-5760]. for structural studies, human sp-d was isolated from amniotic fluid by affinity chromatography on maltose-sepharose followed by f.p.l.c. on superose 6, which showed it to have a molecular mass of approx. 620 kda in non-dissociating conditions. on sds/ ...	1992	1339284
similarity of chlamydia pneumoniae strains in the variable domain iv region of the major outer membrane protein gene.	dna was amplified by polymerase chain reaction from the gene encoding the major outer membrane protein (momp) of chlamydia pneumoniae in order to examine the relatedness of strains isolated from diverse geographical regions. primers for this reaction were chosen to span a 207-bp region comparable to that of the fourth variable segment of the momp gene of chlamydia trachomatis. among c. trachomatis, sequence heterogeneity is characteristic within variable sequence domain iv (vdiv) and correlates ...	1992	1339411
[the prevalence of chlamydia trachomatis infections in gynecologic diseases].		1992	1340263
[urogenital infections produced by chlamydia trachomatis].		1992	1340265
neurolymphomatosis: a clinicopathologic syndrome re-emerges.	we describe a patient with sensorimotor peripheral neuropathy and cranial neuropathy due to autopsy-proven neurolymphomatosis defined by infiltration of peripheral nerves by tumor cells and review the findings in 39 previously reported patients. the cause of the neuropathy is not known. the association with immune-deficient states suggests virally mediated pathogenesis, possibly a retrovirus.	1986	1340762
the malaria control program in papua new guinea: epidemiological surveillance and a look into the future.	the four major phases of the malaria control program in papua new guinea are briefly described. routine indicators utilized during phases ii and iii (the periods when residual indoor spraying was used) are presented and their limitations outlined. the new epidemiological indicators developed during phase iv and now included in the national health plan (1991-1995) are presented. their future use and implications for the malaria control program are analyzed and discussed.	1986	1341083
calcification of bioprosthetic heart valves: a perspective on models.		1986	1341214
a history of endoscopic surgery.	laparoscopic cholecystectomy has become the procedure of choice for the treatment of symptomatic gallbladder disease. this had led to a flurry of activity and a widespread interest in laparoscopic and thoracoscopic procedures. both surgical endoscopy and gallbladder surgery had their beginnings in the 1800s. "modern" surgical endoscopes were first developed in the early 1800s, and cholecystotomy was first performed in indianapolis, indiana by dr. john s. bobbs. a brief history of endoscopes, end ...	1986	1341495
recurrent tonsillitis. the role of chlamydia and mycoplasma.	the core tissue microflora of 40 patients who underwent tonsillectomies were examined with cultures, dna probe tests, enzyme immunoassays, and direct immunofluorescence antibody tests for chlamydia and mycoplasma. we believe this is the largest and most accurate prospective study that has examined the role of chlamydia and mycoplasma in the core tonsil tissue of patients with recurrent or chronic tonsillitis. the data strongly indicate that, unlike acute tonsillopharyngitis, chlamydia and mycopl ...	1992	1341878
[clinical laboratory studies in acute chlamydial, gonococcal and nonchlamydial nongonococcal salpingitis].	the authors carried out a study on 135 patients, hospitalized for acute salpingitis, based on data of history, clinical and laboratory examination. the latter were analysed regarding the isolated etiological agent.	1992	1342144
[cervical and abdominal microbiology in infertile patients].	the microbiology of twenty-nine asymptomatic infertile women was evaluated prospectively. samples were taken from the cervix and the endosalpinx during laparoscopy. cultures for aerobes, anaerobes, neisseria gonorrhoeae, mycoplasma, ureaplasma urealyticum and chlamydia trachomatis were performed. no association was found between the presence of germs in the abdomen or cervix and tubal abnormalities. history of previous iud use was associated with tubal disease.	1992	1342453
[our experience in the diagnosis of chlamydial cervicitis with the chlamydiasyme and chlamyset antigen tests].	the authors analyse in the present study the results of the examination of 309 female patients for chlamydial infection, using chlamyset antigen (orion diagnostica--finland) and chlamydiasyme diagnostic kit (abbott labs--usa). chlamydial antigen was found in the endocervix of 15.2% of the examined cases. comparing the enzyme immunomethod chlamydiasyme with the immunofluorescent method chlamyset antigen the authors found 94.4% diagnostic accuracy.	1992	1342550
nonculture tests for genital tract chlamydial infection. what does the package insert mean, and will it mean the same thing tomorrow?		1992	1344066
challenge of chlamydia research.	chlamydia is an obligate intracellular bacterial pathogen that severely challenges the patience and creativity of all its investigators--even to the point that some investigators have forsaken this field for more productive and fertile areas of research. the two principal difficulties that touch every aspect of chlamydial research are (a) that chlamydiae only grow within eukaryotic host cells and (b) there are limited genetic approaches available. despite these technical difficulties, the fundam ...	1992	1344667
[diagnosis of ocular chlamydiosis by gene amplification (polymerase chain reaction or p.c.r.)].	identification of conjunctival chlamydioses could be very difficult since this disease is extremely latent. moreover previous instillation of topical antibiotic provokes a negativation of d.i.f. and of cultures. p.c.r. is very useful, it was demonstrative in 41 cases out of 47 cases; d.i.f. was positive only in 18 cases. p.c.r. is a very promising method to recognize chlamydiae; identification of different serovars is processing.	1992	1344966
berberine, a potential drug for trachoma.	the clinical serological response to topical treatment of trachoma with berberine an indigenous drug has been studied in 32 microbiologically confirmed cases. efficacy of berberine 0.2% when compared to sulfacetamide 20% was found to be superior in both the clinical course of trachoma and in achieving a fall in the serum antibody titers (p < 0.05) against chlamydia trachomatis in the treated patients.	1992	1344968
[sensitivity and specificity of mouse peritoneal macrophages in the diagnosis of chlamydia trachomatis infections].	we analyzed the sensitivity and specificity of mouse peritoneal macrophages (mpm) for the diagnosis of infection by chlamydia trachomatis. fifty seven samples were studied, 25 of them were from cervix and 32 were from bronchial aspiration, resulting 29 positive by immunofluorescence in mccoy cells and only 16 in mpm. the sensitivity and specificity for mpm were 55% and 96% respectively. we concluded that mccoy cells are the election cells for the diagnosis of chlamydia infection. however, the mp ...	1992	1345308
extrathymic positive selection of alpha beta t-cell precursors in nude mice.	t lymphocytes expressing alpha beta t-cell receptors with sufficient affinity to major histocompatibility complex (mhc) molecules expressed on thymus epithelial cells are positively selected and mature to functional t cells. but several studies have demonstrated that athymic nude mice grafted with mhc-incompatible thymuses developed t cells specific for nude host rather than thymic mhc. we examined this paradox by analysing the specificity of t lymphocytes derived from nude mice. we report here ...	1985	1346064
elderly drivers.		1985	1346320
genetic cause of leukocyte adhesion molecule deficiency. abnormal splicing and a missense mutation in a conserved region of cd18 impair cell surface expression of beta 2 integrins.	patients with leukocyte adhesion molecule (cd11/cd18, beta 2 integrins) deficiency have structural defects in the common beta subunit (cd18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections. to elucidate the nature of these defects in a patient with partial (type ii) deficiency, abnormal cd18 cdna clones were isolated, using the polymerase chain reaction to amplify the patient's b cell-derived cdnas. sequ ...	1992	1346613
a common precursor for cd4+ t cells producing il-2 or il-4.	to investigate whether cd4+ t cells are predetermined to produce a given pattern of lymphokines, we have used a culture system that allows the controlled induction of either il-2- or il-4-producing cd4+ t cells. single, freshly isolated murine cd4+ t cells were activated with con a, ril-2, and apc; the developing clones were split and then cultured for an additional 14 days with either ril-2 alone or with ril-2 and anti-cd3 stimulation. subclones expanded in the presence of ril-2 alone produced ...	2006	1346619
pertussis toxin in the a10 region increases dopamine synthesis and metabolism.	inhibitory regulation of dopamine neurons is mediated by dopamine autoreceptor and gamma-aminobutyric acidb receptor opening of potassium channels. increased potassium conductance by either receptor is g protein dependent. to evaluate the role of g proteins in vivo, pertussis toxin (ptx) was microinjected into the a10 dopamine region and changes in dopamine metabolism and synthesis measured. ptx produced an elevation in dopamine metabolism and synthesis in the a10 region and nucleus accumbens fo ...	1992	1346627
neuroprotective effects of the n-methyl-d-aspartate receptor antagonists ifenprodil and sl-82,0715 on hippocampal cells in culture.	the n-methyl-d-aspartate (nmda) antagonists ifenprodil and sl-82,0715 were examined for neuroprotective efficacy against glutamate toxicity of hippocampal neurons in culture. hippocampal cells were grown on 96-well culture plates for 2 to 3 weeks and then exposed for a 15-min period to glutamate or nmda. neurodegeneration was quantified 24 hr after the excitotoxin exposure, by measuring the activity of lactate dehydrogenase leaked into the culture medium by the damaged cells. glutamate induced a ...	1992	1346650
comparison of enteral nutrition and drug treatment in active crohn's disease: results of the european cooperative crohn's disease study iv.		2006	1346656
cereal-based oral rehydration solutions.		2006	1347356