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analysis of plasmid-mediated quinolone resistance genes in clinical isolates of the tribe proteeae from argentina: first report of qnrd in the americas.to analyse the occurrence and prevalence of plasmid-mediated quinolone resistance (pmqr) genes in the tribe proteeae, 81 isolates (65 proteus spp., 12 morganella morganii and 4 providencia stuartii) consecutively collected in 66 hospitals belonging to the whonet-argentina resistance surveillance network were studied. of the 81 isolates, 50 (62%) were susceptible to quinolones [43/65 (66%) proteus spp. and 7/12 (58%) m. morganii). the remaining 31 isolates (22 proteus spp., 5 m. morganii and all ...201427873695
antioxidant, anticholinesterase and antibacterial activities of stachys guyoniana and mentha aquatica.stachys guyoniana noë ex. batt. and mentha aquatica l. are two algerian lamiaceae used in folk medicine.201727927090
identification of new bacteria harboring qnrs and aac(6')-ib/cr and mutations possibly involved in fluoroquinolone resistance in raw sewage and activated sludge samples from a full-scale wwtp.wastewater treatment plants (wwtps) harbor bacteria and antimicrobial resistance genes, favoring gene exchange events and resistance dissemination. here, a culture-based and metagenomic survey of qnra, qnrb, qnrs, and aac(6')-ib genes from raw sewage (rs) and activated sludge (as) of a full-scale municipal wwtp was performed. a total of 96 bacterial isolates were recovered from nalidixic acid-enrichment cultures. bacteria harboring the aac(6')-ib gene predominated in rs, whereas qnrs-positive is ...201727984803
purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties.the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ...201728110033
fatal rhabdomyolysis caused by morganella morganii in a patient with multiple myeloma.a 64-year-old japanese man with multiple myeloma was admitted to our institute due to fever and hypotension. he had received multiple courses of chemotherapy just before his febrile episode. blood culturing detected morganella morganii. at the time of the diagnosis, his laboratory findings revealed massive rhabdomyolysis with a significantly increased creatinine kinase level (ck; 3,582 u/l); 98.8% of which corresponded to the ck-mb isotype. we diagnosed the patient with sepsis caused by m. morga ...201728154285
extended-spectrum beta-lactamase- and carbapenemase-producing enterobacteriaceae among ethiopian children.infections by extended-spectrum beta-lactamase- (esbl) and carbapenem-resistant enterobacteriaceae (cre) are an emerging problem in children nowadays. hence, the aim of this study was to determine the prevalence of esbl- and carbapenemase-producing enterobacteriaceae among children suspected of septicemia and urinary tract infections (utis).201728182124
high carbapenem resistance in clinical gram-negative pathogens isolated in egypt.the emergence and spread of carbapenem-resistant gram-negative bacteria poses a serious threat to human health worldwide. currently, little is known about the molecular mechanisms underlying carbapenem resistance and their prevalence among gram-negative bacteria in egypt. in this study, we analyzed carbapenemase production in gram-negative bacteria isolated from hospitalized patients in egypt in 2014. all isolates were subjected to phenotypic and genotypic susceptibility testing for carbapenem r ...201728191865
early and late endophthalmitis following trabeculectomy in a chinese population.to investigate the rate of bleb-related endophthalmitis over 5 years in a chinese population.201728221505
characterization of complex, heterogeneous lipid a samples using hplc-ms/ms technique ii. structural elucidation of non-phosphorylated lipid a by negative-ion mode tandem mass spectrometry.non-phosphorylated lipid a species confer reduced inflammatory potential for the bacteria. knowledge on their chemical structure and presence in bacterial pathogens may contribute to the understanding of bacterial resistance and activation of the host innate immune system. in this study, we report the fragmentation pathways of negatively charged, non-phosphorylated lipid a species under low-energy collision-induced dissociation conditions of an electrospray ionization quadrupole time-of-flight i ...201628239963
very high prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae in bacteriemic patients hospitalized in teaching hospitals in bamako, mali.the worldwide dissemination of extended-spectrum beta-lactamase producing enterobacteriaceae, (esbl-e) and their subset producing carbapenemases (cpe), is alarming. limited data on the prevalence of such strains in infections from patients from sub-saharan africa are currently available. we determined, here, the prevalence of esbl-e/cpe in bacteriemic patients in two teaching hospitals from bamako (mali), which are at the top of the health care pyramid in the country. during one year, all entero ...201728245252
serratia marcescens, morganella morganii, klebsiella oxytoca related peritonitis attacks in a patient on automated peritoneal dialysis: a case report. 201728256286
impact of revised broad-spectrum cephalosporin clinical and laboratory standards institute breakpoints on susceptibility in enterobacteriaceae producing ampc β-lactamase.we evaluated the impact of revised clinical and laboratory standards institute (clsi) breakpoints for broad-spectrum cephalosporins (bscs) on the susceptibilities of 1,742 isolates of enterobacter species, serratia marcescens, citrobacter freundii, and morganella morganii. the 2011 clsi criteria for cefotaxime and ceftazidime reduced the rates of susceptibility by 2.9% and 5.9%, respectively. the 2014 clsi criteria for cefepime reduced the rate of susceptibility by 13.9%, and categorized 11.8% i ...201728271652
a case of sulfhemoglobinemia in a child with chronic constipation.sulfhemoglobinemia is a rare condition in which a sulfur atom oxidizes the heme moiety in hemoglobin, making the hemoglobin incapable of carrying oxygen and leading to hypoxia and cyanosis. this condition has been described in patients taking sulfur medications or who have cultured hydrogen sulfide producing intestinal bacteria such as morganella morganii. this case describes a pediatric patient who was found to have cyanosis on two occasions of urinary tract infection in the setting of chronic ...201728348950
successful treatment of pd peritonitis due to morganella morganii resistant to third-generation cephalosporins - a case report.morganella morganii is a rare cause of peritonitis in patients on peritoneal dialysis (pd). most of the reported cases have resorted to a switch to hemodialysis. we herein report a case of peritonitis due to m. morganii resistant to third-generation cephalosporins, which was treated successfully with intraperitoneal (ip) tobramycin followed by oral ciprofloxacin. early microbiologic diagnosis is essential in the treatment of peritonitis from rare microorganisms such as morganella morganii, and a ...201728360373
biofilm colonization in chronic treatment refractory infections presenting with discharging sinuses: a study in a tertiary care hospital of eastern india.treatment refractory chronic recurrent infections mean those chronic infections which recur by same causal agents with similar drug responsiveness after apparent relief following full course of recommended antimicrobial management.201728367029
characterization and genomic analyses of two newly isolated morganella phages define distant members among tevenvirinae and autographivirinae subfamilies.morganella morganii is a common but frequent neglected environmental opportunistic pathogen which can cause deadly nosocomial infections. the increased number of multidrug-resistant m. morganii isolates motivates the search for alternative and effective antibacterials. we have isolated two novel obligatorily lytic m. morganii bacteriophages (vb_mmom_mp1, vb_mmop_mp2) and characterized them with respect to specificity, morphology, genome organization and phylogenetic relationships. mp1's dsdna ge ...201728387353
wound infection secondary to snakebite.snakebites can produce severe local and systemic septic complications as well as being associated with significant overall morbidity and even mortality.201728395682
giant iliopsoas abscess caused by morganella morganii.background while uncommon, iliopsoas abscesses can become the underlying cause of a fever of unknown origin. even in such cases, it is considered rare for an iliopsoas abscess to extend into the subcutaneous space. case report a 74-year-old woman with a history of schizophrenia was referred to our hospital with a high-grade fever. the patient was unaware of her febrile status prior to admission. there was no previous hospital admission. examination revealed a non-tender mass in the lower right b ...201728404984
multidrug resistance salmonella genomic island 1 in a morganella morganii subsp. morganii human clinical isolate from france.salmonella genomic island 1 (sgi1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous salmonella enterica serovars and also in proteus mirabilis. a serious threat to public health was revealed in the recent description in p. mirabilis of a sgi1-derivative multidrug resistance island named pgi1 (proteus genomic island 1) carrying extended-spectrum-β-lactamase (esbl) and metallo-β-lactamase resist ...201728435889
the susceptibility to fosfomycin of gram-negative bacteria isolates from urinary tract infection in the czech republic: data from a unicentric study.against a background of rapid increase of β-lactamase-producing or multi-resistant pathogenic bacteria and the resulting lack of effective antibiotic treatment, some older antibiotics have been tested for new therapeutic uses. one of these is fosfomycin, to which according to studies these resistant bacteria are very sensitive. our study was designed because there is no data on the fosfomycin susceptibility rate in the czech republic.201728446177
gut microbiota species can provoke both inflammatory and tolerogenic immune responses in human dendritic cells mediated by retinoic acid receptor alpha ligation.dendritic cells are considered as the main coordinators of both mucosal and systemic immune responses, thus playing a determining role in shaping the outcome of effector cell responses. however, it is still uncovered how primary human monocyte-derived dc (modc) populations drive the polarization of helper t (th) cells in the presence of commensal bacteria harboring unique immunomodulatory properties. furthermore, the individual members of the gut microbiota have the potential to modulate the out ...201728458670
dissemination of bla oxa-370 gene among several enterobacteriaceae species in brazil.oxa-370 is a recently described oxa-48 variant that has only been described in a few enterobacter spp. and klebsiella pneumoniae isolates. the purpose of this study is to assess the prevalence of oxa-370-producing isolates in carbapenem-nonsusceptible enterobacteriaceae recovered from 28 hospitals from brazil. real-time pcr was used to determine the presence of bla ndm-1, bla kpc-2, bla vim-type, bla ges-type, bla oxa-48-like, and bla imp-type genes. a total of 4,451 enterobacteriaceae were scre ...201728555403
a cost-saving strategy for processing isolated uropathogens in community-acquired urinary tract infections.outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. the aim of this study was compare cost and efficiency to process uropathogens between microscan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). in the first period, a total 9918 bacterial populations were isolated from urine samples. annual estimated costs during 2010 and 2011 for processing were eur 53,818 and eur 57,306, r ...201728559163
[adequate antibiotic prophylaxis and therapy of periprosthetic infections of the breast - review of 468 consecutive implant removals].background periprosthetic infections are feared complications in aesthetic as well as in reconstructive breast surgery. the purpose of our study was to evaluate our institution's specific culture data and to identify most common organisms and suitable antibiotics for prophylaxis and first line treatment in implant-based breast surgery. patients and methods we analyzed all patients with a change or removal of breast implants in the period from 01.01.2012 to 31.12.2015 retrospectively. based on th ...201728561169
igm-mediated autoimmune responses to oxidative specific epitopes, but not nitrosylated adducts, are significantly decreased in pregnancy: association with bacterial translocation, perinatal and lifetime major depression and the tryptophan catabolite (trycat) pathway.immunoglubulin (ig)m responses directed to oxidative specific epitopes (oses) and nitric oxide (no)-adducts are significantly associated with major depression and physio-somatic symptoms. end of term serum igm responses to oses and no-adducts were assayed in pregnant women with (n = 24) and without prenatal depression (n = 25) as well as in 24 non-pregnant women. associations of igm/iga responses to gram-negative gut commensal bacteria (leaky gut index) and iga/igm responses to tryptophan catabo ...201728600633
investigation of acid phosphatase variants for the synthesis of phosphate monoesters.the major drawback of using phosphatases for transphosphorylation reactions lies in product depletion caused by the natural hydrolytic activity of the enzymes. variants of phoc-mm from morganella morganii and nsap-eb from escherichia blattae were studied for their ability to maintain a high product level in the transphosphorylation of various primary alcohols. a single amino acid exchange delivered phosphatase variant phoc-mm g92d, which was able to catalyze the phosphorylation of primary alcoho ...201728600898
draft genome sequences of extended-spectrum-beta-lactamase-producing morganella morganii strains aa1 and av1, isolated from a freshwater lake and eicchorniacrassipes roots.two strains of morganella morganii, aa1 and av1, were isolated from freshwater and eicchornia crassipes roots, respectively. here, we report their draft genome sequences, which are ~3.6 mb and have 51% g+c content. the predicted coding sequences (3,259 for strain aa1 and 3,345 for strain av1) encode beta-lactamases, transpeptidases, and penicillin-binding proteins.201728619802
bacterial genus is a risk factor for major amputation in patients with diabetic foot.to evaluate whether bacterial genus is a risk factor for major amputation in patients with diabetic foot and infected ulcer.201728658333
bilateral morganella morganii keratitis in a patient with facial topical corticosteroid-induced rosacea-like dermatitis: a case report.bilateral keratitis rarely occurs in individuals without predisposing factors. here we describe the clinical course of a patient who developed a bilateral keratitis caused by morganella. morganii which might be associated with long term using of topical corticosteroids-containing preparations on the face.201728659135
prophylactic use of intraoperative vancomycin powder and postoperative infection: an analysis of microbiological patterns in 1200 consecutive surgical cases.objective wound infections following spinal surgery for deformity place a high toll on patients, providers, and the health care system. the prophylactic application of intraoperative vancomycin powder has been shown to lower the infection risk after thoracolumbar decompression and fusion for deformity correction. the purpose of this study was to assess the microbiological patterns of postoperative surgical site infections (ssis) after prophylactic use of vancomycin powder in adult patients under ...201728665245
heat resistance of histidine decarboxylase from gram-negative histamine-producing bacteria in seafood.precooking of tuna is a potential critical control point (ccp) in the commercial manufacturing of canned tuna. to assess the efficacy of precooking as a ccp, an understanding of the thermal properties of histamine-producing bacteria (hpb) and their histidine decarboxylase (hdc) enzymes is required. the thermal properties of many hpb have been determined, but the thermal resistances of the hdc enzymes are unknown. the purpose of this study was to determine the d- and z-values of selected hdc enzy ...201728696146
honeybee (apis mellifera)-associated bacterial community affected by american foulbrood: detection of paenibacillus larvae via microbiome analysis.honeybee (apis mellifera l.) workers act as passive vectors of paenibacillus larvae spores, which cause the quarantine disease american foulbrood (afb). we assessed the relative proportions of p. larvae within the honeybee microbiome using metabarcoding analysis of the 16 s rrna gene. the microbiome was analyzed in workers outside of the afb zone (control - afb0), in workers from asymptomatic colonies in an afb apiary (afb1), and in workers from colonies exhibiting clinical afb symptoms (afb2). ...201728698604
development of silver nanoparticles loaded chitosan-alginate constructs with biomedical potentialities.herein, a facile biosynthesis of silver nanoparticles (agnps) and agnps-loaded chitosan-alginate constructs with biomedical potentialities is reported. the uv-vis spectroscopic profile confirmed the synthesis of agnps using methanolic leaves extract of euphorbia helioscopia. the newly developed agnps were characterized using various analytical and imaging techniques including uv-vis and ft-ir spectroscopy, x-ray diffraction (xrd), scanning electron microscopy (sem), energy dispersive spectroscop ...201728705499
assessment of cultivable oral bacterial flora from important venomous snakes of india and their antibiotic susceptibilities.snakebite is a common, frequently devastating, occupational, socio-economic hazard, and it has a great impact on the rural population of india. snakebite is a major cause of the human morbidity and mortality since ancient times, as it not only affects the victim by systemic envenomation but also by wound infections originating from deadly pathogenic microorganisms from the oral cavity of the offending snake. the pathogens from the oral cavity of the snake tend to initiate an infection, resulting ...201728733908
high prevalence of extended-spectrum β-lactamase-producing enterobacteriaceae in wild fish from the mediterranean sea in algeria.we investigated the prevalence of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae among wild fish from the coast of bejaia (algeria) in the mediterranean sea.201728805537
necrotizing fasciitis following venomous snakebites in a tertiary hospital of southwest taiwan.necrotizing fasciitis following venomous snakebites is uncommon. the purpose of this study was to describe the initial clinical features of necrotizing fasciitis after snakebites, and to identify the risk factors for patients with cellulitis who later developed necrotizing fasciitis.201728811229
isolation, identification and characterization of morganella morganii from naja naja atra in beijing, china.morganella morganii is an important opportunistic human pathogen and belongs to the family of enterobacteriaceae. although it is widely distribution, it only be considered a rare cause of human infections. we report the isolate of m. morganii from naja naja atra following infections of heart, lung and liver. seven strains were confirmed using 16s rdna amplified and sequences. antimicrobial susceptibility testing of m. morganii isolates demonstrated ubiquitous resistance to ampicillin, amoxicilli ...201728838340
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