Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| intracellular growth in acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of legionella pneumophila. | since legionella pneumophila is an intracellular pathogen, entry into and replication within host cells are thought to be critical to its ability to cause disease. l. pneumophila grown in one of its environmental hosts, acanthamoeba castellanii, is phenotypically different from l. pneumophila grown on standard laboratory medium (bcye agar). although amoeba-grown l. pneumophila displays enhanced entry into monocytes compared to bcye-grown bacteria, the mechanisms of entry used and the effects on ... | 1999 | 10456883 |
| invasive ability of an escherichia coli strain isolated from the ileal mucosa of a patient with crohn's disease. | crohn's disease (cd) is an inflammatory bowel disease in which escherichia coli strains have been suspected of being involved. we demonstrated previously that ileal lesions of cd are colonized by e. coli strains able to adhere to intestinal caco-2 cells but devoid of the virulence genes so far described in the pathogenic e. coli strains involved in gastrointestinal infections. in the present study we compared the invasive ability of one of these strains isolated from an ileal biopsy of a patient ... | 1999 | 10456892 |
| cloning and characterization of a gene encoding an antigenic membrane protein from actinobacillus pleuropneumoniae with homology to abc transporters. | actinobacillus pleuropneumoniae is a pathogenic bacterium responsible for a highly contagious and often fatal form of bronchopneumonia in swine. survival from a natural infection generally results in immunity from further infection by all 12 common serotypes, suggesting the presence of common protective antigens. we have identified one of the antigenic membrane proteins from a. pleuropneumoniae serotype 5, and cloned the gene which encodes it. this gene is found in all 12 serotypes, and encodes ... | 1999 | 10459579 |
| molecular epidemiological investigation of an outbreak of campylobacter jejuni identifies a dominant clonal line within scottish serotype hs55 populations. | three molecular typing methods, pulsed-field gel electrophoresis (pfge), ribotyping, and flagellin (flaa) gene typing, were used to discriminate within a group of 28 campylobacter jejuni, heat-stable serotype 55 (hs55) isolates derived from cases of campylobacter enteritis occurring throughout scotland, including 9 isolates associated with an outbreak. pfge was found to be most discriminatory, identifying 6 distinct profiles, followed by ribotyping (5 profiles), and then flagellin gene typing (4 ... | 1999 | 10459638 |
| an outbreak of campylobacter food poisoning at a university campus. | twelve cases of campylobacter jejuni infection were identified on a university campus in the first 12 days of november 1997. consumption of food from a single outlet, where poor food handling practices were identified, was statistically associated with infection. the epidemiological evidence suggested that what presented as a point source outbreak was a series of small cross contamination incidents associated with multiple strain types. the nature of this outbreak suggests that current surveilla ... | 1999 | 10462894 |
| a novel approach for the construction of a campylobacter mutant library. | given the lack of functional transposons for use in campylobacter spp., an alternative method of insertional mutagenesis using natural transformation was developed. high efficiencies of transformation were only obtained with species-specific dna. this feature was a key element in the construction of mutant libraries of this bacterium. a chromosomal library of campylobacter jejuni 81116 dna was made in shuttle vector puoa18. next, a kanamycin-resistance (kmr) cassette was ligated into the inserts ... | 1999 | 10463181 |
| direct analysis and identification of helicobacter and campylobacter species by maldi-tof mass spectrometry. | campylobacter jejuni, campylobacter fetus, and campylobacter coli were compared with helicobacter pylori and helicobacter mustelae by direct analysis of individual cultured colonies in 50% methanol-water with a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (maldi-tof ms). h. pylori and campylobacter species from blood agar culture produced unique, complex spectra with over 25 different ions in mass/charge (m/z) range from 2,000 to 62,000. a biomarker for h. pylori ... | 1999 | 10464475 |
| anti-lactoferrin antibodies and other types of anti-neutrophil cytoplasmic antibodies (anca) in reactive arthritis and ankylosing spondylitis. | fifty-five serum samples from patients with reactive arthritis (rea), 40 from patients with ankylosing spondylitis (as) and three from patients with chronic sacroiliac joint arthritis were analysed for the presence of anca of igg class by means of enzyme immunosorbent assay using lactoferrin (lf), myeloperoxidase (mpo) and antigen extracted from azurophil granules ('alpha-antigen') containing proteinase 3 (pr3) as substrate. igg-anca were found in 31 (56%) patients with rea. twenty-three (42%) h ... | 1999 | 10469064 |
| sulfonamide resistance in clinical isolates of campylobacter jejuni: mutational changes in the chromosomal dihydropteroate synthase. | the characterization of the genetic basis of sulfonamide resistance in campylobacter jejuni was attempted. the resistance determinant from a sulfonamide-resistant strain of c. jejuni was cloned and was found to show 42% identity with the folp gene (which codes for dihydropteroate synthase, the target of sulfonamides) of the related bacterium helicobacter pylori. the sequences of the areas surrounding the folp gene in c. jejuni showed similarity to those of the areas surrounding the corresponding ... | 1999 | 10471557 |
| cloning and expression of an immunogenic membrane-associated protein of helicobacter hepaticus for use in an enzyme-linked immunosorbent assay. | helicobacter hepaticus is a bacterial pathogen that causes chronic active hepatitis and inflammatory bowel disease in mice. the purpose of this study was to develop a recombinant antigen-based enzyme-linked immunosorbent assay (elisa) to detect h. hepaticus-infected mice. a genomic library of h. hepaticus was constructed and was screened with sera from h. hepaticus-infected mice. a 459-bp open reading frame that coded for an 18-kda immunoreactive protein, map18, was identified. the gene had high ... | 1999 | 10473529 |
| genetics of o-antigen biosynthesis in pseudomonas aeruginosa. | pathogenic bacteria produce an elaborate assortment of extracellular and cell-associated bacterial products that enable colonization and establishment of infection within a host. lipopolysaccharide (lps) molecules are cell surface factors that are typically known for their protective role against serum-mediated lysis and their endotoxic properties. the most heterogeneous portion of lps is the o antigen or o polysaccharide, and it is this region which confers serum resistance to the organism. pse ... | 1999 | 10477307 |
| metabolism and genetics of helicobacter pylori: the genome era. | the publication of the complete sequence of helicobacter pylori 26695 in 1997 and more recently that of strain j99 has provided new insight into the biology of this organism. in this review, we attempt to analyze and interpret the information provided by sequence annotations and to compare these data with those provided by experimental analyses. after a brief description of the general features of the genomes of the two sequenced strains, the principal metabolic pathways are analyzed. in particu ... | 1999 | 10477311 |
| helicobacter pylori physiology predicted from genomic comparison of two strains. | helicobacter pylori is a gram-negative bacteria which colonizes the gastric mucosa of humans and is implicated in a wide range of gastroduodenal diseases. this paper reviews the physiology of this bacterium as predicted from the sequenced genomes of two unrelated strains and reconciles these predictions with the literature. in general, the predicted capabilities are in good agreement with reported experimental observations. h. pylori is limited in carbohydrate utilization and will use amino acid ... | 1999 | 10477312 |
| systemic lupus erythematosus demonstrating serum anti-gm1 antibody, with sudden onset of drop foot as the initial presentation. | in systemic lupus erythematosus (sle), peripheral neuropathies are relatively uncommon and rarely present as the initial symptom. we herein describe a 61-year-old woman who developed a sudden onset of drop foot, which was indistinguishable from guillain-barré syndrome based on the clinical symptoms alone. antibodies against ganglioside gm1 were detected in the serum, while no antibodies to campylobacter jejuni were observed. an electrophysiological study showed axonal impairment rather than demy ... | 1999 | 10480305 |
| campylobacter bacteremia and pneumonia in two splenectomized patients. | two patients with beta thalassemia who had undergone splenectomy for hypersplenism were admitted to the hospital with high fever and lobar pneumonia. neither patient had gastrointestinal symptoms. campylobacter bacteremia was diagnosed in both patients. campylobacter jejuni was identified in the first patient and campylobacter fetus in the second. both patients were treated with broad-spectrum antibiotics and recovered fully. | 1999 | 10482027 |
| holliday junction processing in bacteria: insights from the evolutionary conservation of ruvabc, recg, and rusa. | 1999 | 10482492 | |
| hp0333, a member of the dpra family, is involved in natural transformation in helicobacter pylori. | helicobacter pylori is naturally competent for dna transformation, but the mechanism by which transformation occurs is not known. for haemophilus influenzae, dpra is required for transformation by chromosomal but not plasmid dna, and the complete genomic sequence of h. pylori 26695 revealed a dpra homolog (hp0333). examination of genetic databases indicates that dpra homologs are present in a wide variety of bacterial species. to examine whether hp0333 has a function similar to dpra of h. influe ... | 1999 | 10482496 |
| the c-terminal domain of the bordetella pertussis autotransporter brka forms a pore in lipid bilayer membranes. | brka is a 103-kda outer membrane protein of bordetella pertussis that mediates resistance to antibody-dependent killing by complement. it is proteolytically processed into a 73-kda n-terminal domain and a 30-kda c-terminal domain as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. brka is also a member of the autotransporter family of proteins. translocation of the n-terminal domain of the protein across the outer membrane is hypothesized to occur through a pore formed by ... | 1999 | 10482528 |
| identification of a functional fur gene in bradyrhizobium japonicum. | the recent identification of the iron response regulator (irr) in bradyrhizobium japonicum raised the question of whether the global regulator fur is present in that organism. a fur gene homolog was isolated by the functional complementation of an escherichia coli fur mutant. the b. japonicum fur bound to a fur box dna element in vitro, and a fur mutant grown in iron-replete medium was derepressed for iron uptake activity. thus, b. japonicum expresses at least two regulators of iron metabolism. | 1999 | 10482529 |
| recombinant single-chain fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization. | using phage-display technology, a recombinant single-chain fv antibody fragment (scfv) was rapidly generated from the k16-16 hybridoma secreting mouse monoclonal antibody (mab) that binds to acetylaminofluorene-labeled dna (aaf-dna). the selected a4 phage-scfv specifically bound to aaf-dna. the anti-aaf scfv gene was then recloned into a fusion vector for the production of a hybrid protein comprising the antibody fragment fused to a potent bacterial alkaline phosphatase variant (phoav). the anti ... | 1999 | 10485265 |
| [acute demyelinating motor neuropathy: an atypical form of the guillain-barre syndrome?]. | a 33-year-old man presented an acute motor demyelinating neuropathy following campylobacter jejuni enteritis. the patient was improved with an igiv treatment. clinical features and course time were compatible with the diagnosis of a guillain-barré syndrome. the electrophysiologic studies were in favor of multifocal motor neuropathy with conduction blocks. we discuss the nosologic group of this neuropathy. | 1999 | 10486851 |
| phage typing of campylobacter jejuni and campylobacter coli and its use as an adjunct to serotyping. | campylobacter is the most commonly reported cause of gastro-intestinal infection in england and wales, with over 50,000 reported cases in 1997. the majority of human campylobacter isolates in england and wales are c. jejuni (c. 90%) with most of the remainder being c. coli. we describe the use of phage typing as an extension to serotyping for more detailed characterization within these two species. the scheme was piloted during a study of 2407 c. jejuni and 182 c. coli strains isolated in wales ... | 1999 | 10487641 |
| amplified-fragment length polymorphism analysis: the state of an art. | 1999 | 10488158 | |
| ciprofloxacin resistance in campylobacter jejuni isolates: detection of gyra resistance mutations by mismatch amplification mutation assay pcr and dna sequence analysis. | the gyra quinolone resistance determining region was sequenced from 13 ciprofloxacin-resistant and 20 ciprofloxacin-susceptible campylobacter jejuni isolates. all isolates resistant to ciprofloxacin had thr-86-to-ile mutations, a mutation frequently associated with the acquisition of resistance to fluoroquinolones. a mismatch amplification mutation assay (mama) pcr protocol was developed that detects this gyra mutation in quinolone-resistant isolates. the mama pcr provides a means for routine de ... | 1999 | 10488192 |
| detection of helicobacter pylori antibodies in a pediatric population: comparison of three commercially available serological tests and one in-house enzyme immunoassay. | a serum immunoglobulin g enzyme immunoassay (eia) for helicobacter pylori antibodies already in use in adults was evaluated with 99 pediatric serum samples to determine its usefulness for the study of h. pylori disease in children. the reference method used was either the (13)c-urea breath test or a biopsy culture of gastric mucosa. in children, an eia cutoff of 0.35 absorbancy unit yielded sensitivity, specificity, and positive and negative predictive values of 93, 97, 93, and 97%, respectively ... | 1999 | 10488200 |
| specific pcr identification and differentiation of the thermophilic campylobacters, campylobacter jejuni, c. coli, c. lari, and c. upsaliensis. | a sensitive pcr assay that detects the thermophilic campylobacters c. jejuni, c. coli, c. lari, and c. upsaliensis is reported. furthermore, by digestion of the pcr products with two restriction enzymes, species differentiation was demonstrated. thus, the present method has the potential to be used for both detection and identification of thermophilic campylobacter species. | 1999 | 10488210 |
| antibiotic susceptibility patterns and beta-lactamase production of animal and human isolates of campylobacter in lagos, nigeria. | forty-three stool samples and rectal swabs were collected from diarrhoeic and 20 non-diarrhoeic patients under 5 years of age at various general hospitals in lagos. a total of 110 faecal samples from animals (101 from chickens and 9 from pigs) were obtained from mitchel farm, agege and oshodi--isolo local government farms at ejigbo. campylobacter species were isolated from 6 (13.9%) of 43 children with gastroenteritis and none from 20 asymptomatic subjects. forty-nine (48.5%) isolates from the h ... | 1999 | 10488568 |
| monoclonal antibodies raised against guillain-barré syndrome-associated campylobacter jejuni lipopolysaccharides react with neuronal gangliosides and paralyze muscle-nerve preparations. | guillain-barré syndrome and its variant, miller-fisher syndrome, are acute, postinfectious, autoimmune neuropathies that frequently follow campylobacter jejuni enteritis. the pathogenesis is believed to involve molecular mimicry between sialylated epitopes on c. jejuni lpss and neural gangliosides. more than 90% of miller-fisher syndrome cases have serum anti-gq1b and anti-gt1a ganglioside antibodies that may also react with other disialylated gangliosides including gd3 and gd1b. structural stud ... | 1999 | 10491405 |
| the absence of cecal colonization of chicks by a mutant of campylobacter jejuni not expressing bacterial fibronectin-binding protein. | campylobacter jejuni is a common cause of human gastrointestinal illness throughout the world. infections with c. jejuni and campylobacter coli are frequently acquired by eating undercooked chicken. the ability of c. jejuni to become established in the gastrointestinal tract of chickens is believed to involve binding of the bacterium to the gastrointestinal surface. a 37-kd outer membrane protein, termed cadf, has been described that facilitates the binding of campylobacter to fibronectin. this ... | 1999 | 10494431 |
| clostridium botulinum c2 toxin delays entry into mitosis and activation of p34cdc2 kinase and cdc25-c phosphatase in hela cells. | the clostridium botulinum c2 toxin adp-ribosylates monomeric actin, thereby inducing disassembly of actin filaments, alteration of focal adhesions, and rounding of cells. after treatment with c2 toxin, cells stop to proliferate but remain viable for about 2 days. in view of reported correlations between the structure of the actin cytoskeleton and cell cycle transition, the effects of c2 toxin on the g(2)/m phase transition of the cell division cycle were studied. since c2 toxin delayed entry int ... | 1999 | 10496881 |
| in vivo distribution of helicobacter felis in the gastric mucus of the mouse: experimental method and results. | we describe a method that permits the collection of very small samples (2 nl) from precisely defined positions within the gastric mucus of anesthetized mice. this method was used to study the in vivo local distribution of bacteria within the mucus of helicobacter felis-infected mice. a total of 200 samples from 40 mice were analyzed. each sample was microscopically analyzed, within less than 1 min, as a native preparation. to avoid changes in bacterial location within the mucus after collection ... | 1999 | 10496889 |
| mutational analysis of the recj exonuclease of escherichia coli: identification of phosphoesterase motifs. | the recj gene, identified in escherichia coli, encodes a mg(+2)-dependent 5'-to-3' exonuclease with high specificity for single-strand dna. genetic and biochemical experiments implicate recj exonuclease in homologous recombination, base excision, and methyl-directed mismatch repair. genes encoding proteins with strong similarities to recj have been found in every eubacterial genome sequenced to date, with the exception of mycoplasma and mycobacterium tuberculosis. multiple genes encoding protein ... | 1999 | 10498723 |
| interstrain variation of the polysaccharide b biosynthesis locus of bacteroides fragilis: characterization of the region from strain 638r. | the sequence and analysis of the capsular polysaccharide biosynthesis locus, ps b2, of bacteroides fragilis 638r are described, and the sequence is compared with that of the ps b1 biosynthesis locus of b. fragilis nctc 9343. two genes of the region, wcgd and wcgc, are shown by complementation to encode a udp-n-acetylglucosamine 2-epimerase and a udp-n-acetylmannosamine dehydrogenase, respectively. | 1999 | 10498737 |
| outbreak of escherichia coli o157:h7 and campylobacter among attendees of the washington county fair-new york, 1999. | september 3, 1999, the new york state department of health (nysdoh) received reports of at least 10 children hospitalized with bloody diarrhea or escherichia coli o157:h7 infection in counties near albany, new york. all of the children had attended the washington county fair, which was held august 23-29, 1999; approximately 108,000 persons attended the fair during that week. subsequently, fair attendees infected with campylobacter jejuni also were identified. an ongoing investigation includes he ... | 1999 | 10499785 |
| pathogenesis of guillain-barré and miller fisher syndromes subsequent to campylobacter jejuni enteritis. | some patients developed guillain-barré syndrome (gbs) after the administration of bovine gangliosides. patients with gbs subsequent to campylobacter jejuni enteritis frequently have igg antibody to gm1 ganglioside. miller fisher syndrome (mfs), a variant of gbs, is associated with igg antibody to gq1b ganglioside. we showed the existence of molecular mimicry between gm1 and lipopolysaccharide of c. jejuni isolated from patients with gbs, and that between gq1b and c. jejuni lipopolysaccharides fr ... | 1999 | 10507987 |
| cold shock proteins and low-temperature response of streptococcus thermophilus cnrz302. | low-temperature adaptation and cryoprotection were studied in the thermophilic lactic acid bacterium streptococcus thermophilus cnrz302. s. thermophilus actively adapts to freezing during a pretreatment at 20 degrees c, resulting in an approximately 1, 000-fold increased survival after four freeze-thaw cycles compared to mid-exponential-phase cells grown at an optimal temperature of 42 degrees c. no adaptation is observed when cells are exposed to a temperature (10 degrees c) below the minimal g ... | 1999 | 10508072 |
| helicobacter species colonizing pig stomach: molecular characterization and determination of prevalence. | the infection rate of 60 pigs (10 pigs from each of six farms) by helicobacter species was studied by two techniques. histological examination of the cardiac area of the stomach yielded a 58% positive result versus an 80% positive result by pcr with genus-specific primers. eighty percent of the 16s rrna gene was amplified, classified in four groups by pcr-restriction fragment length polymorphism, and sequenced. isolates from all farms except one (farm c) were identified as helicobacter heilmanni ... | 1999 | 10508105 |
| viability and dna maintenance in nonculturable spiral campylobacter jejuni cells after long-term exposure to low temperatures. | survival of campylobacter jejuni at 4 and 20 degrees c was investigated by using cellular integrity, respiratory activity, two-dimensional (2d) protein profile, and intact dna content as indicators of potential viability of nonculturable cells. intact dna content after 116 days, along with cellular integrity and respiring cells, was detected for up to 7 months at 4 degrees c by pulsed-field gel electrophoresis. most changes in 2d protein profiles involved up- or down-regulation. | 1999 | 10508106 |
| [campylobacter jejuni enteritis and guillain-barré syndrome]. | some patients developed guillain-barré syndrome after the administration of bovine brain ganglioside. patients with guillain-barré syndrome subsequent to campylobacter jejuni enteritis frequently have igg antibody to gm1 ganglioside. miller fisher syndrome, a variant of guillain-barré syndrome, is associated with igg antibody to gq1b ganglioside. my colleagues and i showed the existence of molecular mimicry between gm1 and lipopolysaccharide of c. jejuni isolated from a patient with guillain-bar ... | 1999 | 10511801 |
| opening the iron box: transcriptional metalloregulation by the fur protein. | 1999 | 10515908 | |
| campylobacter jejuni contains two fur homologs: characterization of iron-responsive regulation of peroxide stress defense genes by the perr repressor. | expression of the peroxide stress genes alkyl hydroperoxide reductase (ahpc) and catalase (kata) of the microaerophile campylobacter jejuni is repressed by iron. whereas iron repression in gram-negative bacteria is usually carried out by the fur protein, previous work showed that this is not the case in c. jejuni, as these genes are still iron repressed in a c. jejuni fur mutant. an open reading frame encoding a fur homolog (designated perr for "peroxide stress regulator") was identified in the ... | 1999 | 10515927 |
| [measurement of antiganglioside autoantibodies by immunodot-blot assay: clinical importance in peripheral neuropathies]. | we retrospectively evaluated measurement data and clinical relevance of autoantibodies to gangliosides in peripheral neuropathies (pn). the igg and igm antiganglioside autoantibodies were determined by our own immunodot-blot assay on membrane and by enzyme-linked immunosorbent assay (elisa) in sera of 1,342 patients with peripheral neuropathies. anti-gm1 and anti-gd1b autoantibodies formed a part of the normal autoantibody repertoire and were common place in 12% of normal subjects and in 14% of ... | 1999 | 10518060 |
| transcellular translocation of campylobacter jejuni across human polarised epithelial monolayers. | the mechanisms whereby campylobacter jejuni translocates across the host intestinal epithelium are not yet understood and the transepithelial route remains undefined. during c. jejuni translocation, the transmonolayer electrical resistance (ter) across polarised monolayers of caco-2 cells is not affected and the penetration of [(14)c]inulin across the monolayers does not increase. over 24 h, however, bacteria damage the monolayer integrity, causing a decrease in the ter. these results support c. ... | 1999 | 10518717 |
| routine identification of campylobacter jejuni and campylobacter coli from human stool samples. | correct identification of campylobacter jejuni and campylobacter coli isolates to the species or subspecies level is a cumbersome but nevertheless important task for a routine diagnostic laboratory. the widely used biochemical tests might be often misleading while more sophisticated phenotypic or genotypic methods are not generally available. this investigation was performed to assess the performance of common biochemical identification in comparison with species-specific pcr and gas liquid chro ... | 1999 | 10518720 |
| synthesis of a disialylated hexasaccharide of type viii group b streptococcus capsular polysaccharide. | as part of our program to design, develop and prepare protective vaccines against the bacterial pathogens group b streptococcus, we report the synthesis of a disialylated hexasaccharide. this hexasaccharide represents a portion of the serotype-specific capsular polysaccharide of type viii that has the tetrasaccharide repeat unit [beta-l-rhap-(1-->4)-beta-d-glcp-(1-->4)-[alpha-neu5ac-(2--> 3)]-beta-d- galp-(1-->4)]n. a tetrasaccharide corresponding to this repeat unit has been synthesized by us [ ... | 1999 | 10520252 |
| chemical structure of a polysaccharide from campylobacter jejuni 176.83 (serotype o:41) containing only furanose sugars. | a neutral polysaccharide was obtained by hot phenol-water extraction of biomass from campylobacter jejuni 176.83 and subsequently separated from acid-liberated core oligosaccharide of lipopolysaccharide by sequential gpc on bio-gel p6 and tsk-40 columns. all sugar components of the trisaccharide repeating unit of the polysaccharide were found to be of the furanose ring form. the major trisaccharide contained beta-l-arabinose, 6-deoxy-beta-d-altro-heptose (beta-d-6d-althep) and 6-deoxy-beta-l-alt ... | 1999 | 10520260 |
| case-control study of enteropathogens associated with childhood diarrhea in dhaka, bangladesh. | the international centre for diarrhoeal disease research, bangladesh, is a major center for research into diarrheal diseases. the center treats more than 100,000 patients a year. to obtain useful information representative of all patients, a surveillance system in which a 4% systematic sample of all patients is studied in detail, including etiological agents of diarrhea, was installed in october 1979. the first paper on etiology for the surveillance patients was published in 1982, which identifi ... | 1999 | 10523534 |
| identification of hippurate-negative thermophilic campylobacters. | twenty-eight thermophilic campylobacter isolates showing negative hippurate hydrolysis were further characterized. using campylobacter jejuni and coli specific primers for ceue gene, five of the isolates with repeatedly negative results in rapid hippurate hydrolysis were positive in c. jejuni-specific polymerase chain reaction (pcr) and 13 isolates were shown to be c. coli. all except one isolate with positive results in c. jejuni pcr were negative in c. coli pcr including those with repeatedly ... | 1999 | 10529875 |
| outer membrane protein a-promoted actin condensation of brain microvascular endothelial cells is required for escherichia coli invasion. | escherichia coli is the most common gram-negative bacterium that causes meningitis during the neonatal period. we have previously shown that the entry of circulating e. coli organisms into the central nervous system is due to their ability to invade the blood-brain barrier, which is composed of a layer of brain microvascular endothelial cells (bmec). in this report, we show by transmission electron microscopy that e. coli transmigrates through bmec in an enclosed vacuole without intracellular mu ... | 1999 | 10531228 |
| evaluation of a truncated recombinant flagellin subunit vaccine against campylobacter jejuni. | a recombinant protein comprising the maltose-binding protein (mbp) of escherichia coli fused to amino acids 5 to 337 of the flaa flagellin of campylobacter coli vc167 was evaluated for immunogenicity and protective efficacy against challenge by a heterologous strain of campylobacter, campylobacter jejuni 81-176, in two murine models. the sequence of the flaa gene of strain 81-176 revealed a predicted protein which was 98.1% similar to that of vc167 flaa over the region expressed in the fusion pr ... | 1999 | 10531231 |
| fumarate metabolism and the microaerophily of campylobacter species. | (1) the role of fumarate metabolism in the microaerophily of the campylobacter genus and the effects of therapeutic agents against it were investigated. (2) nmr spectroscopy was employed to determine the properties of campylobacter fumarase (fum) and fumarate reductase (frd). radiotracer analysis was used to determine the production of carbon dioxide by campylobacter cells. standard microbiological techniques were used to measure the effects of environmental conditions and inhibitors on bacteria ... | 1999 | 10533287 |
| primary immunodeficiency diseases. report of an iuis scientific committee. international union of immunological societies. | 1999 | 10540200 | |
| the oenococcus oeni clpx homologue is a heat shock gene preferentially expressed in exponential growth phase. | using degenerated primers from conserved regions of previously studied clpx gene products, we cloned the clpx gene of the malolactic bacterium oenococcus oeni. the clpx gene was sequenced, and the deduced protein of 413 amino acids (predicted molecular mass of 45,650 da) was highly similar to previously analyzed clpx gene products from other organisms. an open reading frame located upstream of the clpx gene was identified as the tig gene by similarity of its predicted product to other bacterial ... | 1999 | 10542163 |
| probiotics shown to change bacterial community structure in the avian gastrointestinal tract. | culturing and molecular techniques were used to monitor changes in the bacterial flora of the avian gastrointestinal (gi) tract following introduction of genetically modified (gm) and unmodified probiotics. community hybridization of amplified 16s ribosomal dna demonstrated that the bacterial flora of the gi tract changed significantly in response to the probiotic treatments. the changes were not detected by culturing. although both gm and non-gm strains of enterococcus faecium ncimb 11508 chang ... | 1999 | 10543832 |
| recovery in embryonated eggs of viable but nonculturable campylobacter jejuni cells and maintenance of ability to adhere to hela cells after resuscitation. | the existence of a viable but nonculturable (vbnc) state has been described for campylobacter jejuni as it had been for a number pathogenic bacteria. three c. jejuni human isolates were suspended in surface water and subsequently entered the vbnc state. after starvation for 30 days, vbnc cells were inoculated in the yolk sacs of embryonated eggs. culturable cells were detected in a large proportion of the embryonated eggs inoculated with vbnc c. jejuni cells. recovered cells kept their adhesion ... | 1999 | 10543837 |
| eighty three years of the guillain-barré syndrome: clinical and immunopathologic aspects, current and future treatments. | it is now 83 years that guillain, barré and strohl described the first two cases of an acute paralytic illness with the typical "dissociation albumino-cytologique" in the cerebrospinal fluid. since then, the full spectrum of gbs has been documented in hundreds of cases ranging from acute inflammatory demyelinating polyneuropathy to the pure motor variants and the miller fisher syndrome. during the last 10 years, detailed immunopathologic features have been described and new triggering and possib ... | 1999 | 10546299 |
| longevity of pathogenic bacteria especially salmonella in cistern water. | cistern-collected rain water which is used as process water for toilet flushing, cleaning purposes, garden irrigation and linen washing, was experimentally contaminated with pathogenic bacteria, salmonella enteritidis, yersinia enterocolitica and campylobacter jejuni. the longevity of those pathogens was investigated considering different temperatures and contents of organic materials. neither salmonella enteritidis, yersinia enterocolitica nor campylobacter jejuni are able to grow at temperatur ... | 1999 | 10546329 |
| campylobacter jejuni among patients with gastroenteritis: incidence at a reference microbiology laboratory in san juan, puerto rico. | a study designed to evaluate the incidence of campylobacter jejuni infection among patients with gastroenteritis referred to a microbiology laboratory in san juan was conducted from december 1998 through may 1999. | 1999 | 10547872 |
| campylobacter jejuni. | campylobacter jejuni is the most frequently diagnosed bacterial cause of human gastroenteritis in the united states. the emergence of antimicrobial-resistant and, in particular, of fluoroquinolone-resistant c. jejuni infections in europe and the united states, temporally associated with the approval of use of fluoroquinolones in veterinary medicine, is an important public health concern. recent research has provided strong evidence for an association between campylobacter infection and guillain- ... | 1999 | 10549422 |
| molecular cloning and characterization of the helicobacter pylori flid gene, an essential factor in flagellar structure and motility. | helicobacter pylori colonizes the human stomach and can cause gastroduodenal disease. flagellar motility is regarded as a major factor in the colonizing ability of h. pylori. the functional roles of flagellar structural proteins other than flaa, flab, and flge are not well understood. the flid operon of h. pylori consists of flag, flid, and flis genes, in the order stated, under the control of a sigma(28)-dependent promoter. in an effort to elucidate the function of the flid protein, a hook-asso ... | 1999 | 10559162 |
| novel bacterium isolated from a lung transplant patient with cystic fibrosis. | the major clinical problem for patients with cystic fibrosis (cf) is progressive loss of pulmonary function, usually due to chronic bacterial infections. a patient with cf and a lung transplant was severely infected with a previously unidentified gram-negative bacterium. we isolated this organism (strain ds15158) from the patient and characterized it by phylogenetic analysis of the small-subunit rrna and biochemically by the biolog gn microplate assay, fatty acid analysis, and various standard l ... | 1999 | 10565895 |
| large-scale survey of campylobacter species in human gastroenteritis by pcr and pcr-enzyme-linked immunosorbent assay. | a pcr-based study of the incidence of enteropathogenic campylobacter infection in humans was done on the basis of a detection and identification algorithm consisting of screening pcrs and species identification by pcr-enzyme-linked immunosorbent assay. this was applied to dna extracted from 3,738 fecal samples from patients with sporadic cases of acute gastroenteritis, submitted by seven regional public health laboratories in england and wales over a 2-year period. the sending laboratories had c ... | 1999 | 10565897 |
| predominant staphylococcus aureus isolated from antibiotic-associated diarrhea is clinically relevant and produces enterotoxin a and the bicomponent toxin luke-lukd. | staphylococcus aureus was isolated as the predominant or only isolate from cultures of stools of 60 patients over 2 years in a university hospital, leading to the collection of 114 isolates. diarrhea was observed in 90% of the patients. ninety-eight percent of the patients had received antibiotics in the month before the diarrhea. ninety-two percent of the s. aureus isolates were methicillin resistant. s. aureus was encountered with antibiotic-associated diarrhea among 47 quite elderly patients ... | 1999 | 10565923 |
| rapid identification of campylobacter, arcobacter, and helicobacter isolates by pcr-restriction fragment length polymorphism analysis of the 16s rrna gene. | a rapid two-step identification scheme based on pcr-restriction fragment length polymorphism (pcr-rflp) analysis of the 16s rrna gene was developed in order to differentiate isolates belonging to the campylobacter, arcobacter, and helicobacter genera. for 158 isolates (26 reference cultures and 132 clinical isolates), specific rflp patterns were obtained and species were successfully identified by this assay. | 1999 | 10565952 |
| cytolethal distending toxin of haemophilus ducreyi induces apoptotic death of jurkat t cells. | the immune response to haemophilus ducreyi is mediated in part by t cells infiltrating the site of infection. in this study, we show that h. ducreyi antigen preparations inhibited the proliferation of peripheral blood mononuclear cells and primary human t-cell lines. h. ducreyi also inhibited jurkat t-cell proliferation and induced apoptosis of jurkat t cells, confirmed through the detection of dna degradation and membrane unpacking. the cytotoxic product(s) was present in cell-free culture supe ... | 1999 | 10569755 |
| identification of a novel mycobacterial histone h1 homologue (hupb) as an antigenic target of panca monoclonal antibody and serum immunoglobulin a from patients with crohn's disease. | panca is a marker antibody associated with inflammatory bowel disease (ibd), including most patients with ulcerative colitis and a subset with crohn's disease. this study addressed the hypothesis that panca reacts with an antigen(s) of microbial agents potentially relevant to ibd pathogenesis. using a panca monoclonal antibody, we have previously identified the c-terminal basic random-coil domain of histone h1 as a panca autoantigen. blast analysis of the peptide databases revealed h1 epitope ho ... | 1999 | 10569769 |
| microtubules are associated with intracellular movement and spread of the periodontopathogen actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans suny 465, the invasion prototype strain, enters epithelial cells by an actin-dependent mechanism, escapes from the host cell vacuole, and spreads intracellularly and to adjacent epithelial cells via intercellular protrusions. internalized organisms also egress from host cells into the assay medium via protrusions that are associated with just a single epithelial cell. here we demonstrate that agents which inhibit microtubule polymerization (e.g., colchicine) ... | 1999 | 10569770 |
| campylobacter upsaliensis gastroenteritis in childhood. | campylobacter upsaliensis can cause gastroenteritis and bacteremia. data on its epidemiology and role in pediatric gastroenteritis are limited. | 1999 | 10571436 |
| wagging the dog: antibiotic use and the emergence of resistance. | 1999 | 10571711 | |
| helicobacter pylori rocf is required for arginase activity and acid protection in vitro but is not essential for colonization of mice or for urease activity. | arginase of the helicobacter pylori urea cycle hydrolyzes l-arginine to l-ornithine and urea. h. pylori urease hydrolyzes urea to carbon dioxide and ammonium, which neutralizes acid. both enzymes are involved in h. pylori nitrogen metabolism. the roles of arginase in the physiology of h. pylori were investigated in vitro and in vivo, since arginase in h. pylori is metabolically upstream of urease and urease is known to be required for colonization of animal models by the bacterium. the h. pylori ... | 1999 | 10572136 |
| improved pcr detection of campylobacter jejuni from chicken rinses by a simple sample preparation procedure. | many food samples and enrichment media are inhibitory to the pcr, thereby lowering its detection capacity. a simple sample preparation method based on buoyant density centrifugation was examined for its application in pcr detection of campylobacter jejuni from chicken rinse samples. bacterial cells were spiked at different levels in a mixture of preston broth and chicken rinse (4:1 ratio) and 0.9 ml of these mixtures were layered over 0.6 ml of gradient medium made from percoll. pcr sensitivity ... | 1999 | 10573390 |
| prevalence of campylobacter spp. in poultry and poultry meat in germany. | of 509 samples from poultry flocks, 209 isolates (41.1%) were campylobacter positive. the number of positive cases in broiler carcasses was 45.9%. of 52 pheasants investigated, 25.9% were campylobacter positive. campylobacter jejuni was isolated from 86 (42.0%) poultry flock samples, 47 (43%) broiler samples and 15 (28%) wild pheasant samples. c. coli was found at a rate of 1.2% in poultry flocks, 13% in broilers and 21% in pheasants. | 1999 | 10574094 |
| cloning, sequencing, and characterization of genomic subtracted sequences from listeria monocytogenes. | individual sequences of a genomic subtracted, pcr-amplified, mixed-sequence probe (gs probe) were cloned and sequenced. the gs probe differentiated restriction fragment length polymorphism patterns for listeria monocytogenes but did not hybridize with members of other bacterial genera. sequence analysis identified several l. monocytogenes sequences already present in the genbank database; the putative identities of other sequences were inferred from homology data, and still other sequences did n ... | 1999 | 10583999 |
| arginine biosynthesis in campylobacter jejuni tgh9011: determination of the argcobd cluster. | arginine biosynthetic genes from campylobacter jejuni tgh9011 were cloned by functional complementation of the respective escherichia coli arginine biosynthetic mutants. complementation of arga, argb, argc, argd, arge, argf, and argh auxotrophs was accomplished using a pbr322-based c. jejuni tgh9011 plasmid library. by cross-complementation analyses, the first four steps of arginine biosynthesis were shown to be closely linked on the genome. two additional clones complementing the first (arga) a ... | 1999 | 10588044 |
| [the hypothesis of infectious etiology for idiopathic nervous system diseases: from the postulates of koch to the criteria of hill]. | the evaluation of the hypothesis of an infectious etiology to some neurological diseases comprises four different situations. first, numerous neurological diseases have an obvious infectious etiology (encephilitis, meningoencephilitis). second, some neurological disorders were primarily suspected to be have an infectious etiology, but the causative microorganism was discovered either longtime after the princeps description of the disease (neurologic whipple disease, due to tropheryma whippelii), ... | 1999 | 10598368 |
| rapidly progressive, predominantly motor guillain-barré syndrome with anti-galnac-gd1a antibodies. | to investigate the presence of anti-galnac-gd1a antibodies in patients with guillain-barré syndrome (gbs) and to determine the relation of anti-ganglioside antibodies with clinical features. | 1999 | 10599792 |
| a multifunctional atp-binding cassette transporter system from vibrio cholerae transports vibriobactin and enterobactin. | vibrio cholerae uses the catechol siderophore vibriobactin for iron transport under iron-limiting conditions. we have identified genes for vibriobactin transport and mapped them within the vibriobactin biosynthetic gene cluster. within this genetic region we have identified four genes, viup, viud, viug and viuc, whose protein products have homology to the periplasmic binding protein, the two integral cytoplasmic membrane proteins, and the atpase component, respectively, of other iron transport s ... | 1999 | 10601218 |
| mics of mutacin b-ny266, nisin a, vancomycin, and oxacillin against bacterial pathogens. | peptide antibiotics, particularly lantibiotics, are good candidates for replacing antibiotics to which bacteria have become resistant. in order to compare two such lantibiotics with two antibiotics, the mics of nisin a, mutacin b-ny266, vancomycin, and oxacillin against various bacterial pathogens were determined. the results indicate that nisin a and mutacin b-ny266 are as active as vancomycin and oxacillin against most of the strains tested. furthermore, mutacin b-ny266 remains active against ... | 2000 | 10602718 |
| cytolethal distending toxin sequence and activity in the enterohepatic pathogen helicobacter hepaticus. | little is known about the molecular pathogenesis of hepatitis and enterocolitis caused by enterohepatic helicobacter species. sonicates of the murine pathogen helicobacter hepaticus were found to cause progressive cell distension, accumulation of filamentous actin, and g(2)/m cell cycle arrest in hela cell monolayers. the genes encoding this cytotoxic activity were cloned from h. hepaticus. three open reading frames with closest homology to cdta, cdtb, and cdtc from campylobacter jejuni were ide ... | 2000 | 10603386 |
| detection of antibodies against campylobacter jejuni serogroup pen o:19 purified flagellar protein in a patient with guillain-barré syndrome. | c. jejuni serogroup pen o:19 was isolated from a stool specimen from a patient with guillain-barré syndrome (gbs). flagellar protein was isolated and purified from reference strain c. jejuni pen o:19, atcc 43,446, as well as from a homologous patient strain. antibodies against flagellar protein were detected by means of immunoblotting, enzyme-linked immunosorbent assay (elisa) and tube agglutination test. the antibody titres were found to be directly correlated at the beginning and in the recove ... | 1999 | 10603661 |
| a phylogenomic study of dna repair genes, proteins, and processes. | the ability to recognize and repair abnormal dna structures is common to all forms of life. studies in a variety of species have identified an incredible diversity of dna repair pathways. documenting and characterizing the similarities and differences in repair between species has important value for understanding the origin and evolution of repair pathways as well as for improving our understanding of phenotypes affected by repair (e.g., mutation rates, lifespan, tumorigenesis, survival in extr ... | 1999 | 10606811 |
| dose response and organ invasion of day-of-hatch leghorn chicks by different isolates of campylobacter jejuni. | colonization of the ceca and organ invasion by different isolates of campylobacter jejuni were investigated in day-of-hatch leghorn chicks. this model of campylobacter colonization of the ceca demonstrates that 1) day-of-hatch birds do not naturally contain cecal campylobacter, 2) ceca can be colonized with c. jejuni by oral gavage and not by cloacal inoculation; 3) c. jejuni can be recovered from the ceca up until at least 7 days postinoculation, 4) cecal colonization occurs when as little as 1 ... | 1999 | 10611992 |
| severe guillain-barré syndrome associated with campylobacter jejuni infection. | 1999 | 10612373 | |
| inhibition of colonisation of the alimentary tract in young chickens with campylobacter jejuni by pre-colonisation with strains of c. jejuni. | strains of campylobacter jejuni, isolated from human gastro-intestinal infection and inoculated orally into 1-day-old chicks, colonised the alimentary tract (caecum) well. there was evidence of invasion from the intestine to the spleen. oral inoculation with some but not all strains of c. jejuni 24 h earlier (within 12 h of hatching) prevented establishment by challenge strains administered orally 1 day later. one strain which was less able to colonise the gut was less inhibitory than other stra ... | 2000 | 10612737 |
| o-acetylserine sulfhydrylase from methanosarcina thermophila. | cysteine is the major source of fixed sulfur for the synthesis of sulfur-containing compounds in organisms of the bacteria and eucarya domains. though pathways for cysteine biosynthesis have been established for both of these domains, it is unknown how the archaea fix sulfur or synthesize cysteine. none of the four archaeal genomes sequenced to date contain open reading frames with identities to either o-acetyl-l-serine sulfhydrylase (oass) or homocysteine synthase, the only sulfur-fixing enzyme ... | 2000 | 10613861 |
| localized reversible frameshift mutation in the flha gene confers phase variability to flagellin gene expression in campylobacter coli. | phase variation of flagellin gene expression in campylobacter coli ua585 was correlated with high-frequency, reversible insertion and deletion frameshift mutations in a short homopolymeric tract of thymine residues located in the n-terminal coding region of the flha gene. mutation-based phase variation in flha may generate functional diversity in the host and environment. | 2000 | 10613882 |
| pathophysiology of campylobacter jejuni infections of humans. | campylobacter jejuni and closely related organisms are major causes of human bacterial enteritis. these infections can lead to extraintestinal disease and severe long-term complications. of these, neurological damage, apparently due to the immune response of the host, is the most striking. this review examines current knowledge of the pathophysiology of the organism. diversity of c. jejuni isolates in genotypic and phenotypic characteristics now is recognized and clinically relevant examples are ... | 1999 | 10617934 |
| prevalence of toxin types and colonization factors in enterotoxigenic escherichia coli isolated during a 2-year period from diarrheal patients in bangladesh. | the prevalence of toxin types and colonization factors (cfs) of enterotoxigenic escherichia coli (etec) was prospectively studied with fresh samples (n = 4,662) obtained from a 2% routine surveillance of diarrheal stool samples over 2 years, from september 1996 to august 1998. stool samples were tested by enzyme-linked immunoassay techniques and with specific monoclonal antibodies for the toxins and cfs. the prevalence of etec was 14% (n = 662), with over 70% of the strains isolated from childre ... | 2000 | 10618058 |
| detection of clarithromycin-resistant helicobacter pylori strains by a preferential homoduplex formation assay. | it has been shown that resistance to clarithromycin, a major cause of failure in helicobacter pylori eradication therapy, is associated with point mutations in the 23s rrna gene. we sought to apply the preferential homoduplex formation assay (phfa), a novel technique for the efficient detection of point mutations, to detection of the mutations. phfa was performed on streptavidin-coated microtiter plates with biotin- and dinitrophenyl-labeled amplicons to detect the wild-type gene or each mutant ... | 2000 | 10618089 |
| prevalence of campylobacter, arcobacter, helicobacter, and sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for campylobacters. | the aims of this study were to investigate the prevalence of campylobacteria including campylobacter jejuni subsp. jejuni (c. jejuni) and campylobacter coli in human clinical samples and in samples from healthy individuals and to reevaluate the efficacies of conventional selective methods for isolation of campylobacter spp. two charcoal-based selective media, modified charcoal cefoperazone deoxycholate agar (mccda) and cefoperazone-amphotericin-teicoplanin (cat) agar, were compared with skirrow' ... | 2000 | 10618103 |
| evaluation of a combination rapid immunoassay for detection of giardia and cryptosporidium antigens. | a combination cassette format nonenzymatic rapid immunoassay for detection of giardia and cryptosporidium antigens was evaluated by using 556 patient stool specimens from three clinical laboratories. this assay (genzyme diagnostics contrast giardia/cryptosporidium), which can be used with fresh or formalin-fixed specimens, had unadjusted sensitivities and specificities of 96.1 and 98.5% for giardia and 100 and 98.7% for cryptosporidium, respectively, in this study. | 2000 | 10618122 |
| genotyping of campylobacter spp. | 2000 | 10618195 | |
| sources of escherichia coli in a coastal subtropical environment. | sources of escherichia coli in a coastal waterway located in ft. lauderdale, fla., were evaluated. the study consisted of an extensive program of field measurements designed to capture spatial and temporal variations in e. coli concentrations as well as experiments conducted under laboratory-controlled conditions. e. coli from environmental samples was enumerated by using a defined substrate technology (colilert-18). field sampling tasks included sampling the length of the north fork to identify ... | 2000 | 10618229 |
| isolation and characterization of campylobacter jejuni subsp. jejuni from macaroni penguins (eudyptes chrysolophus) in the subantarctic region. | on bird island, south georgia, albatrosses (n = 140), penguins (n = 100), and fur seals (n = 206) were sampled for campylobacter jejuni. c. jejuni subsp. jejuni was recovered from three macaroni penguins (eudyptes chrysolophus). these isolates, the first reported for the subantarctic region, showed low genetic diversity and high similarity to northern hemisphere c. jejuni isolates, possibly suggesting recent introduction to the area. | 2000 | 10618265 |
| virulent strains of helicobacter pylori demonstrate delayed phagocytosis and stimulate homotypic phagosome fusion in macrophages. | helicobacter pylori colonizes the gastric epithelium of approximately 50% of the world's population and plays a causative role in the development of gastric and duodenal ulcers. h. pylori is phagocytosed by mononuclear phagocytes, but the internalized bacteria are not killed and the reasons for this host defense defect are unclear. we now show using immunofluorescence and electron microscopy that h. pylori employs an unusual mechanism to avoid phagocytic killing: delayed entry followed by homoty ... | 2000 | 10620610 |
| the effect of flavophospholipol (flavomycin) and salinomycin sodium (sacox) on the excretion of clostridium perfringens, salmonella enteritidis, and campylobacter jejuni in broilers after experimental infection. | intestinal colonization and shedding of pathogenic bacteria in animal feces is an important factor in both human food safety and animal health. the effect of broiler feed additives flavophospholipol (fpl; flavomycin, bambermycins) and salinomycin sodium (sal; sacox) given singly on the excretion of salmonella enteritidis, campylobacter jejuni, and clostridium perfringens was studied following controlled infection. the incidence of shedding (number of birds with positive fecal cultures) and the d ... | 1999 | 10626641 |
| ganglioside-induced antiganglioside antibodies from a neuropathy patient cross-react with lipopolysaccharides of campylobacter jejuni associated with guillain-barré syndrome. | antiganglioside serum antibodies from a patient treated with gangliosides were examined for cross-reactivity with lipopolysaccharides (lpss) of campylobacter jejuni strains associated with guillain-barré syndrome (gbs). the patient had no preceding infection with c. jejuni and developed chronic progressive motor polyneuropathy following parenteral ganglioside treatment. serum igg antibodies recognised gm1 and gd1b gangliosides as well as asialo-gm1, and cross-reactivity was observed with lpss fr ... | 2000 | 10626671 |
| flagellin gene polymorphism analysis of campylobacter compared with antigen serotyping. | flagellin gene sequence polymorphisms were used to discriminate amongst 53 strains of campylobacter jejuni and c. coli. the campylobacter strains were made up of forty-three strains of campylobacter jejuni and 10 strains of campylobacter coli. the results were analysed in relation to penner serotyping. twenty dna pcr-rflp patterns (genotypes) were identified by analysis of dde i fragment length polymorphisms in flagellin gene (fla a and fla b) polymerase chain reaction (pcr) products. flagellin ... | 1999 | 10627993 |
| mutational analysis of a role for salicylic acid in iron metabolism of mycobacterium smegmatis. | the role of salicylic acid in iron metabolism was examined in two wild-type strains (mc(2)155 and ncimb 8548) and three mutant strains (mc(2)1292 [lacking exochelin], sm3 [lacking iron-dependent repressor protein ider] and s99 [a salicylate-requiring auxotroph derived in this study]) of mycobacterium smegmatis. synthesis of salicylate in sm3 was derepressed even in the presence of iron, as was synthesis of the siderophores exochelin, mycobactin, and carboxymycobactin. s99 was dependent on salicy ... | 2000 | 10629169 |
| flen, a gene that regulates flagellar number in pseudomonas aeruginosa. | the single polar flagellum of pseudomonas aeruginosa plays an important role in the pathogenesis of infection by this organism. however, regulation of the assembly of this organelle has not been delineated. in analyzing the sequence available at the pseudomonas genome database, an open reading frame (orf), flanked by flagellar genes flhf and flia, that coded for a protein (280 amino acids) with an atp-binding motif at its n terminus was found. the orf was inactivated by inserting a gentamicin ca ... | 2000 | 10629180 |