Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a case of maternal herpes simplex virus encephalitis during late pregnancy. | a pregnant 25-year-old woman at 32 weeks' gestation was admitted to an emergency unit after her husband had found her drowsy and with her tongue bitten. the day before admission, the patient had developed a fever of 39 degrees c, was suffering from headaches, was nauseated and had vomited. on admission, she had anterograde and retrograde amnesia, but no somatic neurological deficits were detected. | 2009 | 19129790 |
| cell cycle inhibitor enhances the resolution of hsv-1-induced proinflammatory response in murine microglial cells. | herpes simplex encephalitis remains one of the most devastating intracranial infections despite available antiviral treatment, with sequelae secondary to a persistent inflammatory response. recently, cyclin-dependent kinases (cdks) have been found to act as cellular targets for antiviral drugs. however, the pharmacological effects of cdk inhibitors on glial cell function in herpes simplex encephalitis have not been elucidated. the aim of this work was to determine the influence of olomoucine on ... | 2009 | 19138471 |
| the bipolar filaments formed by herpes simplex virus type 1 ssb/recombination protein (icp8) suggest a mechanism for dna annealing. | herpes simplex virus type 1 encodes a multifunctional protein, icp8, which serves both as a single-strand binding protein and as a recombinase, catalyzing reactions involved in replication and recombination of the viral genome. in the presence of divalent ions and at low temperature, previous electron microscopic studies showed that icp8 will form long left-handed helical filaments. here, electron microscopic image reconstruction reveals that the filaments are bipolar, with an asymmetric unit co ... | 2009 | 19138689 |
| erythema nodosum-like lesions in treated whipple's disease: signs of immune reconstitution inflammatory syndrome. | treatment of systemic infections due to mycobacteria and hiv infection can lead to paradoxical worsening, the immune reconstitution inflammatory syndrome, in a minority of patients. herein we describe a patient with whipple's disease, a chronic systemic inflammatory disease caused by tropheryma whipplei, who developed cutaneous and later ocular disease after initiation of antibiotic therapy. a 42-year-old man with a 12-year history of arthralgias presented with deteriorating health, including we ... | 2009 | 19150271 |
| herpesvirus gb-induced fusion between the virion envelope and outer nuclear membrane during virus egress is regulated by the viral us3 kinase. | herpesvirus capsids collect along the inner surface of the nuclear envelope and bud into the perinuclear space. enveloped virions then fuse with the outer nuclear membrane (nm). we previously showed that herpes simplex virus (hsv) glycoproteins gb and gh act in a redundant fashion to promote fusion between the virion envelope and the outer nm. hsv mutants lacking both gb and gh accumulate enveloped virions in herniations, vesicles that bulge into the nucleoplasm. earlier studies had shown that h ... | 2009 | 19158241 |
| herpes simplex virus utilizes the large secretory vesicle pathway for anterograde transport of tegument and envelope proteins and for viral exocytosis from growth cones of human fetal axons. | axonal transport of herpes simplex virus (hsv-1) is essential for viral infection and spread in the peripheral nervous system of the host. therefore, the virus probably utilizes existing active transport and targeting mechanisms in neurons for virus assembly and spread from neurons to skin. in the present study, we used transmission immunoelectron microscopy to investigate the nature and origin of vesicles involved in the anterograde axonal transport of hsv-1 tegument and envelope proteins and o ... | 2009 | 19176621 |
| gsk983: a novel compound with broad-spectrum antiviral activity. | gsk983, a novel tetrahydrocarbazole, inhibits the replication of a variety of unrelated viruses in vitro with ec(50) values of 5-20 nm. both replication of the adenovirus ad-5 and the polyoma virus sv-40, and episomal maintenance of human papillomaviruses (hpv) and epstein-barr virus (ebv) are susceptible to gsk983. the compound does not inhibit all viruses; herpes simplex virus (hsv-1), human immunodeficiency virus (hiv), and lytic replication of ebv were not susceptible at concentrations below ... | 2009 | 19187793 |
| influence of antisense oligonucleotides targeting tumor necrosis factor-alpha on experimental herpetic-induced chorioretinitis of mouse eye. | to investigate the effect on the pathological process of topical treatment with antisense oligonucleotides (ason) targeting tumor necrosis factor-alpha (tnf-alpha) in experimental herpes simplex virus type-1 (hsv-1)-induced chorioretinitis of mouse eye. | 2009 | 19188750 |
| [bell's palsy: from viral aetiology to diagnostic reality]. | peripheral injury of the facial nerve is a frequent disorder. it is a stressful situation for the patient and it is functionally hazardous for the cornea. facial palsy is due to a lesion involving the facial pontine nucleus or the nerve trunk in its route from the pontocerebellar angle to the parotid. the idiopathic facial paralysis or bell's palsy (bp) is the most common cause but acute facial palsy can also be due to tumors. a rigorous clinical history and examination must be performed to guid ... | 2009 | 19195745 |
| effects of herpes simplex virus amplicon transduction on murine dendritic cells. | the herpes simplex virus (hsv)-based amplicon is a versatile vaccine platform that has been preclinically vetted as a gene-based immunotherapeutic for cancer, hiv, and neurodegenerative disorders. although it is well known that injection of dendritic cells (dcs) transduced ex vivo with helper virus-free hsv amplicon vectors expressing disease-relevant antigens induces antigen-specific immune responses, the cellular receptor(s) by which the amplicon virion gains entry into dcs, as well as the eff ... | 2009 | 19199821 |
| fewer latent herpes simplex virus type 1 and cytotoxic t cells occur in the ophthalmic division than in the maxillary and mandibular divisions of the human trigeminal ganglion and nerve. | following primary infection of the mouth, herpes simplex virus type 1 (hsv-1) travels retrogradely along the maxillary (v2) or mandibular (v3) nerve to the trigeminal ganglion (tg), where it establishes lifelong latency. symptomatic hsv-1 reactivations frequently manifest as herpes labialis, while ocular hsv-1 disease is rare. we investigated whether these clinical observations are mirrored by the distribution of latent hsv-1 as well as cytotoxic t-cell infiltration around the nerve cell bodies ... | 2009 | 19211753 |
| alzheimer's disease-specific tau phosphorylation is induced by herpes simplex virus type 1. | neurofibrillary tangles are one of the main neuropathological features of alzheimer's disease (ad) and are composed of abnormally phosphorylated forms of a microtubule-associated protein called tau. what causes this abnormal phosphorylation is unknown. our previous studies have implicated herpes simplex virus type 1 (hsv1) as an etiological agent in ad, and so we investigated whether infection with this virus induces ad-like tau phosphorylation. here we demonstrate that hsv1 causes tau phosphory ... | 2009 | 19221424 |
| in vitro antioxidant, antimicrobial and anti-herpes simplex virus type 1 activity of phellodendron amurense rupr. from china. | phellodendron amurense rupr. bark extracts were examined for antioxidant activity, antimicrobial activity and antiviral activity on herpes simplex virus type 1 (hsv-1). ethanol extract showed higher content of both total phenolic and flavonoid than aqueous extract. in dpph (2, 2-diphenyl-1-picrylhydrazyl) assay, the concentration providing 50% inhibition (ic(50)) values were 6.73 +/- 0.87 mg/ml and 4.26 +/- 0.59 mg/ml for aqueous and ethanol extracts respectively. ethanol extract had a much high ... | 2009 | 19222122 |
| molecular-genetic pet imaging using an hsv1-tk mutant reporter gene with enhanced specificity to acycloguanosine nucleoside analogs. | imaging 2 different molecular-genetic events in a single subject by pet is essential in a variety of in vivo applications. using herpes simplex virus-1 thymidine kinase (hsv1-tk) mutants with narrower substrate specificities in combination with wild-type hsv1-tk (wthsv1-tk) would enable differential imaging with corresponding radiotracers, namely 2'-deoxy-2'-(18)f-fluoro-5-ethyl-1-beta-d-arabinofuranosyl-uracil ((18)f-feau) and the acycloguanosine derivative 9-(4-(18)f-fluoro-3-[hydroxymethyl]bu ... | 2009 | 19223410 |
| viral supraglottitis in an adult. | 2009 | 19224474 | |
| detection of herpes viruses in children with acute appendicitis. | this study aimed to investigate the incidence of herpes simplex virus (hsv) types-1 and -2, varicella-zoster virus (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv), human herpes virus 6 (hhv-6) and human herpes virus 7 (hhv-7) in childhood acute appendicitis. | 2009 | 19233720 |
| entry of herpes simplex virus 1 and other alphaherpesviruses via the paired immunoglobulin-like type 2 receptor alpha. | herpes simplex virus 1 (hsv-1) enters cells either via fusion of the virion envelope and host cell plasma membrane or via endocytosis, depending on the cell type. in the study reported here, we investigated a viral entry pathway dependent on the paired immunoglobulin-like type 2 receptor alpha (pilralpha), a recently identified entry coreceptor for hsv-1 that associates with viral envelope glycoprotein b (gb). experiments using inhibitors of endocytic pathways and ultrastructural analyses of chi ... | 2009 | 19244335 |
| [fullevir: in vitro and in vivo antiherpetic activity]. | the antiherpetic properties of a fullerene derivative with aminocaproic acid (manufactured by intelfarm co. as fullevir) were studied in in vitro (in sensitive cell cultures) and in vivo (on a murine model of experimental herpetic encephalitis) experiments. fullevir was found to protect tissue culture cells from the cytodestructive action of herpes simplex virus type 1. it was estimated that ed50 = 5.3 microg/ml and ed90 = 29.1 microg/ml. the agent was most effective when it was administered bef ... | 2009 | 19253725 |
| the epstein-barr virus alkaline exonuclease bglf5 serves pleiotropic functions in virus replication. | the epstein-barr virus (ebv) alkaline exonuclease bglf5 has previously been recognized to contribute to immune evasion by downregulating production of hla molecules during virus replication. we have constructed a bglf5-null virus mutant to determine bglf5's functions during ebv viral replication. quantification of virus production in permissive 293 cells carrying a deltabglf5 genome identified a 17- to 21-fold reduction relative to complemented or wild-type controls. detailed monitoring of delta ... | 2009 | 19264771 |
| herpes simplex virus type 1 regulatory protein icp0 aids infection in cells with a preinduced interferon response but does not impede interferon-induced gene induction. | several independent lines of evidence indicate that interferon-mediated innate responses are involved in controlling herpes simplex virus type 1 (hsv-1) infection and that the viral immediate-early regulatory protein icp0 augments hsv-1 replication in interferon-treated cells. however, this is a complex situation in which the experimental outcome is determined by the choice of multiplicity of infection and cell type and by whether cultured cells or animal models are used. it is now known that ne ... | 2009 | 19264774 |
| [update on the treatment of genital herpes]. | genital herpes is a chronic infection characterized by periodic reactivation. it can produce symptomatic disease in the host although asymptomatic viral excretion can also occur. it is currently the main cause of genital ulceration and an important public health problem that has substantial clinical, psychological, and economic repercussions. this review analyzes the currently available therapeutic options and regimens, which are based mainly on systemic use of antiviral agents such as aciclovir ... | 2009 | 19268108 |
| herpes simplex virus type 1 glycoprotein e mediates retrograde spread from epithelial cells to neurites. | in animal models of infection, glycoprotein e (ge) is required for efficient herpes simplex virus type 1 (hsv-1) spread from the inoculation site to the cell bodies of innervating neurons (retrograde direction). retrograde spread in vivo is a multistep process, in that hsv-1 first spreads between epithelial cells at the inoculation site, then infects neurites, and finally travels by retrograde axonal transport to the neuron cell body. to better understand the role of ge in retrograde spread, we ... | 2009 | 19279108 |
| virion incorporation of the herpes simplex virus type 1 tegument protein vp22 occurs via glycoprotein e-specific recruitment to the late secretory pathway. | the mechanism by which herpesviruses acquire their tegument is not yet clear. one model is that outer tegument proteins are recruited by the cytoplasmic tails of viral glycoproteins. in the case of herpes simplex virus tegument protein vp22, interactions with the glycoproteins ge and gd have been shown. we have previously shown that the c-terminal half of vp22 contains the necessary signal for assembly into the virus. here, we show that during infection vp22 interacts with ge and gm, as well as ... | 2009 | 19279114 |
| isolation and preliminary characterization of herpes simplex virus 1 primary enveloped virions from the perinuclear space. | herpes simplex virus 1 (hsv-1) nucleocapsids exit the nucleus by budding into the inner nuclear membrane, where they exist briefly as primary enveloped virions. these virus particles subsequently fuse their envelopes with the outer nuclear membrane, permitting nucleocapsids to then enter the cytoplasm and complete assembly. we have developed a method to isolate primary enveloped virions from hsv-1-infected cells and subjected the primary enveloped virion preparation to maldi-ms/ms (matrix-assist ... | 2009 | 19279117 |
| kinetics of primary and memory cytotoxic t lymphocyte responses to herpes simplex virus 1 infection: granzyme b mediated ctl activity. | herpes simplex virus type 1 is one of the most common viruses among human population. studies demonstrate the essential role of cell mediated immunity, especially cd8+ t cells, in prevention and clearance of hsv1. | 2009 | 19293474 |
| extreme susceptibility of african naked mole rats (heterocephalus glaber) to experimental infection with herpes simplex virus type 1. | herpes simplex virus type 1 (hsv1) is widely used as a gene delivery vector in a variety of laboratory animals. in a recent study, a thymidine-kinase-inactive (replication-conditional) hsv1 used as a delivery vector was lethal in naked mole rats, whereas mice infected with the identical virus showed no adverse effects. this result prompted us to undertake a controlled comparative histologic study of the effect of hsv1 infection on naked mole rats and mice. replication-competent and replication-c ... | 2009 | 19295058 |
| phenotypic similarities and differences between ul37-deleted pseudorabies virus and herpes simplex virus type 1. | in the absence of the tegument protein pul37, virion formation of pseudorabies virus (prv) and herpes simplex virus type 1 (hsv-1) is severely impaired. non-enveloped nucleocapsids accumulate in clusters in the cytoplasm, whereas only a few enveloped particles can be detected. although a contribution of pul37 to nuclear egress of hsv-1 has been suggested, the nuclear stages of morphogenesis are not impaired in prv-deltaul37-infected cells. moreover, hsv-1 pul37 has been described as essential fo ... | 2009 | 19297610 |
| characterization of an immediate splenic precursor of cd8+ dendritic cells capable of inducing antiviral t cell responses. | mouse spleens contain three major dendritic cell (dc) populations: plasmacytoid dc, conventional cd8(+)cd24(+) dc (cd8(+) dc), and conventional cd8(-)cd24(-) dc (cd8(-) dc). we have previously shown that cd8(+) dc are the major cross-presenting subtype in vivo and are the main inducers of antiviral cytotoxic t lymphocyte responses. here we show that after depletion of cd8(+) dc, the only dc capable of viral ag presentation was a small subset that expresses cd24 but not cd8. this cd8(-)cd24(+) dc ... | 2009 | 19299718 |
| identification of multiple sites suitable for insertion of foreign genes in herpes simplex virus genomes. | information on sites in hsv genomes at which foreign gene(s) can be inserted without disrupting viral genes or affecting properties of the parental virus are important for basic research on hsv and development of hsv-based vectors for human therapy. the intergenic region between hsv-1 ul3 and ul4 genes has been reported to satisfy the requirements for such an insertion site. the ul3 and ul4 genes are oriented toward the intergenic region and, therefore, insertion of a foreign gene(s) into the re ... | 2009 | 19302526 |
| autophagy enhances the presentation of endogenous viral antigens on mhc class i molecules during hsv-1 infection. | viral proteins are usually processed by the 'classical' major histocompatibility complex (mhc) class i presentation pathway. here we showed that although macrophages infected with herpes simplex virus type 1 (hsv-1) initially stimulated cd8(+) t cells by this pathway, a second pathway involving a vacuolar compartment was triggered later during infection. morphological and functional analyses indicated that distinct forms of autophagy facilitated the presentation of hsv-1 antigens on mhc class i ... | 2009 | 19305394 |
| arginine methylation of the icp27 rgg box regulates icp27 export and is required for efficient herpes simplex virus 1 replication. | the herpes simplex virus 1 (hsv-1) multifunctional regulatory protein icp27 shuttles between the nucleus and cytoplasm in its role as a viral mrna export factor. arginine methylation on glycine- and arginine-rich motifs has been shown to regulate protein export. icp27 contains an rgg box and has been shown to be methylated during viral infection. we found by mass spectrometric analysis that three arginine residues within the rgg box were methylated. viral mutants with substitutions of lysine for ... | 2009 | 19321610 |
| regulation of histone deposition on the herpes simplex virus type 1 genome during lytic infection. | during lytic infection by herpes simplex virus type 1 (hsv-1), histones are present at relatively low levels on the viral genome. however, the mechanisms that account for such low levels--how histone deposition on the viral genome is blocked or how histones are removed from the genome--are not yet defined. in this study, we show that histone occupancy on the viral genome gradually increased with time when transcription of the viral immediate-early (ie) genes was inhibited either by deletion of t ... | 2009 | 19321615 |
| towards fibroid gene therapy: adenovirus-mediated delivery of herpes simplex virus 1 thymidine kinase gene/ganciclovir shrinks uterine leiomyoma in the eker rat model. | the objective of this study was to assess in vivo gene therapy of uterine leiomyomas in the eker rat model using adenovirus (ad)-mediated delivery of herpes simplex virus 1 thymidine kinase gene (hsv1tk) followed by ganciclovir (gcv) treatment. | 2009 | 19325244 |
| role of chromatin during herpesvirus infections. | dna viruses have long served as model systems to elucidate various aspects of eukaryotic gene regulation, due to their ease of manipulation and relatively low complexity of their genomes. in some cases, these viruses have revealed mechanisms that are subsequently recognized to apply also to cellular genes. in other cases, viruses adopt mechanisms that prove to be exceptions to the more general rules. the double-stranded dna viruses that replicate in the eukaryotic nucleus typically utilize the h ... | 2009 | 19344747 |
| cross-presentation of viral and self antigens by skin-derived cd103+ dendritic cells. | skin-derived dendritic cells (dcs) include langerhans cells, classical dermal dcs and a langerin-positive cd103(+) dermal subset. we examined their involvement in the presentation of skin-associated viral and self antigens. only the cd103(+) subset efficiently presented antigens of herpes simplex virus type 1 to naive cd8(+) t cells, although all subsets presented these antigens to cd4(+) t cells. this showed that cd103(+) dcs were the migratory subset most efficient at processing viral antigens ... | 2009 | 19349986 |
| [effect of deletion mutant ips-1 on ifn-beta induction and its antiviral to hsv-1]. | the deletion mutant gene of ifn-beta promoter stimulator 1(deltaips-1) was constructed by depleting the amino acid region from 300 to 444 loci and the recombinant plasmid pef-bos-flag/deltaips-1 were constructed to study the effect of deletion mutant deltaips-1 to ifn-beta induction and its antiviral to hsv-1. | 2009 | 19351494 |
| stepwise evolution of the herpes simplex virus origin binding protein and origin of replication. | the herpes simplex virus replicon consists of cis-acting sequences, oris and oril, and the origin binding protein (obp) encoded by the ul9 gene. here we identify essential structural features in the initiator protein obp and the replicator sequence oris, and we relate the appearance of these motifs to the evolutionary history of the alphaherpesvirus replicon. our results reveal two conserved sequence elements in herpes simplex virus type 1, obp; the rvknl motif, common to and specific for all al ... | 2009 | 19351883 |
| physiological transgene regulation and functional complementation of a neurological disease gene deficiency in neurons. | the microtubule-associated protein tau (mapt) and alpha-synuclein (snca) genes play central roles in neurodegenerative disorders. mutations in each gene cause familial disease, whereas common genetic variation at both loci contributes to susceptibility to sporadic neurodegenerative disease. here, we demonstrate exquisite gene regulation of the human mapt and snca transgene loci and functional complementation in neuronal cell cultures and organotypic brain slices using the herpes simplex virus ty ... | 2009 | 19352323 |
| prevention of genital herpes simplex virus type 1 and 2 disease in mice immunized with a gd-expressing dominant-negative recombinant hsv-1. | cj9-gd is a novel herpes simplex virus (hsv) type 1 recombinant virus that is completely replication-defective, expresses high-levels of hsv-1 major antigen glycoprotein d (gd), and can function in trans to inhibit replication of wild-type hsv-1 and hsv-2 in co-infected cells. here, we show that immunization with cj9-gd elicits strong and long-lasting humoral and th1-like cellular immune responses against both hsv-1 and hsv-2. mice immunized with cj9-gd exhibited significant reductions in the ex ... | 2009 | 19357711 |
| immunomodulatory roles of topical dinitrochlorobenzene treatment in herpes simplex virus infection. | epicutaneous application of a contact sensitizer 2, 4-dinitro-1-chlorobenzene (dncb) is known to cause an inflammatory response and a hapten-specific t cell activation. we developed a highly stable gel formulation for dncb which is easy to apply to the skin. the present study focused on whether topical application of dncb gel produced immunomodulation with prophylactic effects against lethal herpes simplex virus infection (hsv) type 1. our results indicated that topical treatment with dncb gel i ... | 2009 | 19362169 |
| induction of sialyl-lex expression by herpes simplex virus type 1 is dependent on viral immediate early rna-activated transcription of host fucosyltransferase genes. | we have previously shown that varicella-zoster virus (vzv) and cytomegalovirus (cmv) infection of diploid human fibroblasts (hel) results in neo-expression of lewis antigens sialyl lewis x (sle(x)) and lewis y (le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltransferase genes (fut1, fut3, fut5, and fut6), whose gene products are responsible for the synthesis of le antigens. here, we show that herpes simplex virus type 1 (hsv-1) also induce ... | 2009 | 19369700 |
| titration of variant hsv1-tk gene expression to determine the sensitivity of 18f-fhbg pet imaging in a prostate tumor. | because of its high selectivity and specificity for the imaging reporter probe 9-(4-(18)f-fluoro-3-[hydroxymethyl]butyl)guanine ((18)f-fhbg), the herpes simplex virus type 1 thymidine kinase (hsv1-tk) variant sr39tk is actively being studied as a pet reporter gene. we recently demonstrated the capability of using a prostate-specific transcriptional amplification pet reporter vector, adtsta-sr39tk, to target prostate cancer lymph node metastasis. however, one area that warrants further study is t ... | 2009 | 19372484 |
| [novel mechanism of herpes simplex virus type-i infection]. | 2009 | 19378786 | |
| pediatric herpes simplex virus keratitis. | to report the clinical characteristics and visual outcomes of pediatric herpes simplex virus (hsv) keratitis. | 2009 | 19387223 |
| hsv-1 encephalitis complicated by cerebral hemorrhage in an hiv-positive person. | although herpes simplex virus type 1 (hsv-1) is the most common cause of sporadic encephalitis in immunocompetent adults, it is an unusual cause of encephalitis in patients with hiv/aids. we report the case of a 56-year-old man with recently diagnosed hiv infection who presented with subacute mental status changes, fever, and temporal lobe abnormalities evident on brain imaging. results of a polymerase chain reaction assay of the cerebrospinal fluid were positive for hsv-1. his course was compli ... | 2009 | 19388182 |
| reactivated herpetic keratitis following laser in situ keratomileusis. | a 40-year-old woman presented with an unusual form of corneal herpetic reactivation 5 weeks after laser in situ keratomileusis. the cornea showed diffuse edema and a stromal ring infiltrate (disciform in appearance). oral antiviral and topical steroids in tapered dosages were administered. the infiltrate disappeared within 6 weeks. progressive improvement was also seen in all the parameters on pentacam scans, including the pachymetry, keratometry readings, and elevation on the anterior float. he ... | 2009 | 19393899 |
| synthesis and biological activity of amino acid esters of acyclovir. | 2009 | 19400144 | |
| induction of multiple pro-inflammatory cytokines by respiratory viruses and reversal by standardized echinacea, a potent antiviral herbal extract. | several viruses associated with upper respiratory diseases have been shown to stimulate the secretion of pro-inflammatory cytokines, including chemokines, sometimes in the absence of viral cytopathology. we evaluated the ability of a standardized preparation of the popular herbal medicine echinacea (echinaforce, an ethanol extract of herb and roots of e. purpurea, and containing known concentrations of marker compounds) to inhibit the viral induction of various cytokines in a line of human bronc ... | 2009 | 19409931 |
| reconstitution of uracil dna glycosylase-initiated base excision repair in herpes simplex virus-1. | herpes simplex virus-1 is a large double-stranded dna virus that is self-sufficient in a number of genome transactions. hence, the virus encodes its own dna replication apparatus and is capable of mediating recombination reactions. we recently reported that the catalytic subunit of the hsv-1 dna polymerase (ul30) exhibits apurinic/apyrimidinic and 5'-deoxyribose phosphate lyase activities that are integral to base excision repair. base excision repair is required to maintain genome stability as ... | 2009 | 19411250 |
| a case of herpetic epithelial keratitis after triamcinolone acetonide subtenon injection. | we report a case of herpes simplex virus (hsv) epithelial keratitis that developed after a subtenon triamcinolone acetonide (ta) injection. | 2009 | 19411969 |
| comparison of the pseudorabies virus us9 protein with homologs from other veterinary and human alphaherpesviruses. | pseudorabies virus (prv) us9 is a small, tail-anchored (ta) membrane protein that is essential for axonal sorting of viral structural proteins and is highly conserved among other members of the alphaherpesvirus subfamily. we cloned the us9 homologs from two human pathogens, varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1), as well as two veterinary pathogens, equine herpesvirus type 1 (ehv-1) and bovine herpesvirus type 1 (bhv-1), and fused them to enhanced green fluorescent ... | 2009 | 19420087 |
| 7-o-methylkaempferol and -quercetin glycosides from the whole plant of nervilia fordii. | five new 7-o-methylkaempferol and -quercetin glycosides, namely, nervilifordins a-e (1-5), were isolated from the whole plant of nervilia fordii, together with seven known flavonoids (6, 7, and 9-13) and one known coumarin (8). their structures were elucidated on the basis of extensive spectroscopic analyses, including hsqc, hmbc, roesy, and chemical methods. compounds 1-3 and 6-13 were evaluated for their anti-herpes simplex virus 1 (hsv-1) activity and cytotoxicity on african green monkey kidn ... | 2009 | 19422205 |
| the herpes simplex virus-induced demise of keratinocytes is associated with a dysregulated pattern of p63 expression. | p63 plays a pivotal role in the development and maintenance of stratified epithelial tissues. in an effort to gain insight into the pathogenic mechanisms of skin infections caused by hsv-1 and hsv-2, we determined the patterns of p63 expression in primary keratinocytes and in the hacat cell line. the levels of deltanp63alpha and a 50kda p73 isoform were decreased, bax-alpha remained unaffected, while the expressions of the bax-beta, tap63gamma and a 44.5kda p73 isoform were highly increased in b ... | 2009 | 19427396 |
| synthesis, antiviral activity, and stability of nucleoside analogs containing tricyclic bases. | a series of 3,9-dihydro-9-oxo-5h-imidazo[1,2-a]purine nucleosides (tricylic nucleosides) were synthesized from 9-[4-alpha-(hydroxymethyl)cyclopent-2-ene-1-alpha-yl]guanine (cbv) 5, (-)-beta-d-(2r,4r)-1,3-dioxolane-guanosine (dxg) 6, 3'-azido-3'-deoxy-guanosine (azg) 7, and 2'-c-methylguanosine 8. their in vitro activity against hiv and hcv was evaluated and correlated to their ability to degrade to their purine counterpart. | 2009 | 19433343 |
| what is your diagnosis? acute herpes simplex virus type 1 stomatitis. | 2009 | 19445307 | |
| sulfated k5 escherichia coli polysaccharide derivatives: a novel class of candidate antiviral microbicides. | antiviral microbicides, topical agents that prevent sexually transmitted infections, mainly work by blocking the interaction between viral proteins and cell surface components. in many instances, virus-cell interaction is mediated by cell surface heparan sulfate proteoglycans (hspgs). hspgs are exploited as attachment receptors by three sexually transmitted viruses: human immunodeficiency virus (hiv), herpes simplex virus (hsv) and human papilloma virus (hpv). since these viruses can either infe ... | 2009 | 19447134 |
| occurrence of hsv-1-induced pneumonitis in patients under standard immunosuppressive therapy for rheumatic, vasculitic, and connective tissue disease. | herpes simplex virus type-1 (hsv-1) has been described to cause respiratory tract infections in critically ill patients or in individuals that are immunocompromised. it is a continuing matter of debate under which circumstances hsv-1 is a relevant pathogen for pneumonitis. while its role during critical illness has been investigated by prospective interventional studies, comparatively little systematic data is available on the role of hsv-1 for pneumonitis in outpatients with autoimmune disease ... | 2009 | 19450259 |
| construction and cellular immune response induction of ha-based alphavirus replicon vaccines against human-avian influenza (h5n1). | several approaches are being taken worldwide to develop vaccines against h5n1 viruses; most of them, however, pose both practical and immunological challenges. one potential strategy for improving the immunogenicity of vaccines involves the use of alphavirus replicons and vp22, a herpes simplex type 1 (hsv-1) protein. in this study, we analysed the antigenic peptides and homogeneity of the ha sequences (human isolates of the h5n1 subtype, from 1997 to 2003) and explored a novel alphavirus replic ... | 2009 | 19450640 |
| isocoumarin glucosides from the scale insect fungus torrubiella tenuis bcc 12732. | three new isocoumarin glucosides (1, 3, and 4), 6,8-dihydroxy-3-methylisocoumarin (2), and 6,8-dihydroxy-3-hydroxymethylisocoumarin (5) were isolated from the scale insect pathogenic fungus torrubiella tenuis bcc 12732. structures of these compounds were elucidated using nmr spectroscopic and ms spectrometric analyses. compound 5 exhibited moderate anti-hsv-1 and antimycobacterial activities with ic(50) and mic values of 50 and 25 microg/ml, respectively. | 2009 | 19456117 |
| [diagnostics of genital herpesvirus infection in women with tubal-peritoneal infertility]. | optimization of diagnostics of genital herpesvirus infection (herpes simplex virus types 1 and 2, cytomegalovirus [cmv]) in women with tubal-peritoneal infertility on the basis of detection of viral material in various parts of genital system using polymerase chain reaction (pcr) and revealing pathogenetically significant systemic abnormalitites of cytokine- and nitroxidergic regulation. | 2009 | 19459485 |
| antiviral activity and mode of action of propolis extracts and selected compounds. | aqueous and ethanol extracts of propolis were analysed phytochemically and examined for their antiviral activity in vitro. different polyphenols, flavonoids and phenylcarboxylic acids were identified as major constituents. the antiviral effect of propolis extracts and selected constituents, e.g. caffeic acid (1), p-coumaric acid (2), benzoic acid (3), galangin (4), pinocembrin (5) and chrysin (6) against herpes simplex virus type 1 (hsv-1) was analysed in cell culture. the 50% inhibitory concent ... | 2010 | 19472427 |
| proline and tyrosine residues in scaffold proteins of herpes simplex virus 1 critical to the interaction with portal protein and its incorporation into capsids. | previous results showed that amino acids 449 to 457 of pu(l)26, a component of the scaffold of herpes simplex virus 1 capsids, were critical for interaction with the portal protein encoded by u(l)6 and for incorporation of the portal into capsids. to identify residues in this scaffold domain critical for the interaction with pu(l)6, the two proteins were coexpressed in the absence of other viral proteins and subjected to immunoprecipitation with scaffold-specific antibody. coimmunoprecipitation ... | 2009 | 19474093 |
| scaffold expulsion and genome packaging trigger stabilization of herpes simplex virus capsids. | herpes simplex virus type 1 (hsv1) capsids undergo extensive structural changes during maturation and dna packaging. as a result, they become more stable and competent for nuclear egress. to further elucidate this stabilization process, we used biochemical and nanoindentation approaches to analyze the structural and mechanical properties of scaffold-containing (b), empty (a), and dna-containing (c) nuclear capsids. atomic force microscopy experiments revealed that a and c capsids were mechanical ... | 2009 | 19487681 |
| development of novel microemulsion-based topical formulations of acyclovir for the treatment of cutaneous herpetic infections. | the present investigation aims at developing microemulsion-based formulations for topical delivery of acyclovir. various microemulsions were developed using isopropyl myristate/captex 355/labrafac as an oil phase, tween 20 as surfactant, span 20 as cosurfactant, and water/dimethylsulfoxide (1:3) as an aqueous phase. transcutol, eucalyptus oil, and peppermint oil were used as permeation enhancers. in vitro permeation studies through laca mice skin were performed using franz diffusion cells. the o ... | 2009 | 19504745 |
| synthesis and antiviral evaluation of 5-(1,2,3-triazol-1-ylmethyl)uridine derivatives. | some 5-(1,2,3-triazol-1-ylmethyl)uridine derivatives were synthesized via the 1,3-dipolar cycloaddition of a 5-azidomethyluridine derivative with substituted acetylenes. the antiviral activities of these compounds against hepatitis a virus (hav, mbb cell culture-adapted strain) and herpes simplex virus type-1 (hsv-1) were tested. | 2009 | 19526706 |
| transient expression of herpes simplex virus type 1 icp22 represses viral promoter activity and complements the replication of an icp22 null virus. | of the five herpes simplex virus type 1 immediate early (ie) proteins, the least is known about the function of icp22 during productive infection and latency. research characterizing the physical and functional properties of the protein has been limited because icp22 has proven to be difficult to express in transient assays. in addition, genetic analysis of icp22 has been complicated by the fact that the c terminus of icp22 is expressed as a discrete protein product. in order to characterize pro ... | 2009 | 19535441 |
| hsv-1-induced socs-1 expression in keratinocytes: use of a socs-1 antagonist to block a novel mechanism of viral immune evasion. | keratinocytes are important for the acute phase of hsv-1 infection and subsequent persistence in sensory nervous tissue. in this study, we showed that keratinocytes (hel-30) were refractory to ifn-gamma induction of an antiviral state to hsv-1 infection, while ifn-gamma did induce an antiviral state in fibroblasts (l929). this led us to examine the possible role of suppressor of cytokine signaling-1 (socs-1) in this refractiveness. rt-pcr analysis of socs-1 mrna expression in hsv-1-infected cell ... | 2009 | 19542368 |
| multivalent binding of carbohydrates by the human alpha-defensin, hd5. | four of the six human alpha-defensins (human neutrophil peptides 1-3 and human alpha-defensin 5; hd5) have a lectin-like ability to bind glycosylated proteins. using hd5 as a model, we applied surface plasmon resonance techniques to gain insights into this property. hd5 bound natural glycoproteins > neoglycoproteins based on bsa > nonglycosylated bsa >> free sugars. the affinity of hd5 for simple sugars covalently bound to bsa was orders of magnitude greater than its affinity for the same sugars ... | 2009 | 19542459 |
| lornoxicam suppresses recurrent herpetic stromal keratitis through down-regulation of nuclear factor-kappab: an experimental study in mice. | we designed the current study to determine the protective effects of lornoxicam, a cyclooxygenase (cox) inhibitor, on recurrent herpetic stromal keratitis (hsk) and the nuclear factor-kappab (nf-kappab)-mediated mechanism in mice. | 2009 | 19547717 |
| hsv-1-derived amplicon vectors: recent technological improvements and remaining difficulties--a review. | amplicons are defective and non-integrative vectors derived from herpes simplex virus type 1. as the vector genome carries no virus genes, amplicons are both non-toxic for the infected cells and non-pathogenic for the inoculated organisms. in addition, the large transgenic capacity of amplicons, which allow delivery of up to 150 kbp of foreign dna, makes these vectors one of the most powerful, interesting and versatile gene delivery platforms. we present here recent technological developments th ... | 2009 | 19547864 |
| soluble cytoplasmic expression, rapid purification, and characterization of cyanovirin-n as a his-sumo fusion. | cyanovirin-n (cvn) is a promising antiviral candidate that has an extremely low sequence homology with any other known proteins. the efficient and soluble expression of biologically functional recombinant cvn (rcvn) is still an obstacle due to insufficient yield, aggregation, and abnormal modification. here, we describe an improved approach to preparing native rcvn from escherichia coli more efficiently. a fusion gene consisting of cvn and sumo (small ubiquitin-related modifier) and a hexahistid ... | 2010 | 19547966 |
| [a kinetic study of gamma interferon production in herpes simplex virus-1 dna prime-protein boost regimen comparing to dna or subunit vaccination]. | the vast majority of the world's population is infected with herpes simplex virus (hsv). although antiviral therapy can reduce the incidence of reactivation and asymptomatic viral shedding, and limit morbidity and mortality from active disease, it cannot cure infection. therefore, the development of an effective vaccine is an important global health priority. in this study, the induction of ifn-gamma production was compared by different herpes simplex virus 1 (hsv1) vaccines. glycoprotein d (gd1 ... | 2009 | 19548528 |
| arginine methylation of the icp27 rgg box regulates the functional interactions of icp27 with srpk1 and aly/ref during herpes simplex virus 1 infection. | the herpes simplex virus 1 protein icp27 is methylated on arginine residues within an rgg box, and arginine methylation regulates icp27 export to the cytoplasm. arginine methylation can regulate protein-protein interactions; therefore, we examined the effect of hypomethylation on icp27's interactions with cellular proteins srpk1 and aly/ref, which bind to icp27 through the rgg box region. during infections with viral mutants containing lysine substitutions or the methylation inhibitor adenosine ... | 2009 | 19553338 |
| oncolytic viral therapy of malignant glioma. | novel approaches to treatment of malignant glioma, the most frequently occurring primary brain tumor, have included the use of a wide range of oncolytic viral vectors. these vectors, either naturally tumor-selective, or engineered as such, have shown promise in the handful of phase i and phase ii clinical trials conducted in recent years. the strategies developed for each of the different viruses currently being studied and the history of their development are summarized here. in addition, the r ... | 2009 | 19560745 |
| identification of a novel herpes simplex virus type 1 transcript and protein (al3) expressed during latency. | the herpes simplex virus type 1 (hsv-1) latency-associated transcript (lat) is abundantly expressed in latently infected sensory neurons. in small animal models of infection, expression of the first 1.5 kb of lat coding sequences is necessary and sufficient for wild-type reactivation from latency. the ability of lat to inhibit apoptosis is important for reactivation from latency. within the first 1.5 kb of lat coding sequences and lat promoter sequences, additional transcripts have been identifi ... | 2009 | 19570955 |
| virucidal activity of polysaccharide extracts from four algal species against herpes simplex virus. | herpes simplex virus types 1 and 2 (hsv-1, hsv-2) infections are common, but can cause serious infections in neonates and the immunocompromised. drugs currently used to treat cutaneous or genital hsv infections are effective in limiting disease, but the emergence of drug resistant viruses in immunocompromised individuals can be problematic. while the prophylactic oral treatment with antiviral drugs can reduce virus shedding and transmission, there is a need for topical microbicides that have the ... | 2009 | 19576248 |
| detection of opportunistic dna viral infections by multiplex pcr among hiv infected individuals receiving care at a tertiary care hospital in south india. | opportunistic viral infections cause increased morbidity and mortality among human immunodeficiency virus (hiv) infected individuals, especially those who are not on antiretroviral treatment. early diagnosis of these opportunistic viruses will be able to reduce the risk of disease progression with appropriate intervention. | 2009 | 19584500 |
| icam-5 modulates cytokine/chemokine production in the cns during the course of herpes simplex virus type 1 infection. | chemokines are important in hse development in the cns but underlying regulatory events are unknown. two-hybrid binding assays identified that intercellular adhesion molecule 5 (icam-5), an immune modulator in the cns, interacted with neurovirulence factor, uol, of hsv-1. viral load and interleukin levels were similar in uol deletion virus (deltauol), and wild type virus infected mouse brains. however, higher numbers of lymphocytes, but unaltered soluble icam-5 and chemokine levels were detected ... | 2009 | 19589604 |
| detection of herpes simplex virus dependent apoptosis. | subversion of the host response to virus infection is a universal theme of virology and viral immunology. multiple mechanisms are in place to limit virus spread on behalf of the host, yet through evolution, viruses have adapted to either weaken or eliminate the effects of these host factors. cell death or apoptosis is one such example of a host response to viral infection. as such, experimental techniques that enable analysis of viruses (and viral genes) involved in triggering, blocking, or perh ... | 2009 | 19609770 |
| herpesviruses--a zoonotic threat? | herpesviruses are highly host specific and share a long synchronous evolution with their hosts. only in rare cases, species barriers fall and allow animal to human or human to animal transmission. among the zoonotic herpesviruses, cercopithecine herpesvirus 1 is the most significant and can be transmitted from macaques to human. conversely, human herpesvirus 1 is capable of causing severe disease in primates. besides those two examples, there are several herpesviruses with a certainly limited or ... | 2010 | 19616388 |
| herpes simplex virus type i infection of mature dendritic cells leads to reduced lmp7-mrna-expression levels. | mature dendritic cells (mdcs) are the most potent antigen presenting cells within the human immune system known today. however, several viruses, including herpes simplex virus type 1 (hsv-1) have developed numerous immune escape mechanisms, such as the avoidance of peptide presentation through the major histocompatibility complex (mhc) class i to cd8(+) cytotoxic t-cells. within the mhc class i pathway, the majority of antigenic peptides are generated by the proteasome, a multicatalytic protease ... | 2009 | 19619915 |
| acute retinal necrosis: a case series with clinical features and treatment outcomes. | to determine clinical features, viral aetiology and treatment outcomes of eyes with acute retinal necrosis (arn). | 2009 | 19624343 |
| rapid tumor cell swelling and bursting: beware of collateral damage. | 2009 | 19644494 | |
| utilizing ras signaling pathway to direct selective replication of herpes simplex virus-1. | re-engineering the tropism of viruses is an attractive translational strategy for targeting cancer cells. the ras signal transduction pathway is a central hub for a variety of pro-oncogenic events with a fundamental role in normal and neoplastic physiology. in this work we were interested in linking ras activation to hsv-1 replication in a direct manner in order to generate a novel oncolytic herpes virus which can target cancer cells. to establish such link, we developed a mutant hsv-1 in which ... | 2009 | 19652721 |
| role of viral chromatin structure in the regulation of herpes simplex virus 1 gene expression and replication. | herpes simplex virus 1 initially infects epithelial cells during the lytic phase of its infectious cycle, followed by establishment of the latent phase within neuronal cells. the two different phases of infection are characterized by distinct gene-expression profiles, involving a temporal gene-expression pattern during the lytic phase succeeded by a complete shutdown of all gene expression, except for one abundant transcript, during the latent phase. the mechanisms controlling these varying degr ... | 2009 | 19659426 |
| inhibition of hsv-1 ocular infection with morpholino oligomers targeting icp0 and icp27. | alternative therapies are needed for hsv-1 infections in patients refractory to treatment with acyclovir (acv) and its derivatives. peptide-conjugated phosphorodiamidate morpholino oligomers (ppmo) are single-stranded dna analogues that enter cells readily and reduce target gene expression through steric blockage of complementary rna. when applied before or soon after infection ppmo targeting the translation-start-site regions of hsv-1 icp0 or icp27 mrna reduced hsv-1 plaque formation by 70-98% ... | 2009 | 19665486 |
| antiviral screening of forty-two egyptian medicinal plants. | egyptian medicinal plants are well known by their diverse uses in traditional folk medicine to cure various ailments including infectious diseases. forty-two egyptian medicinal plant species were selected from local market and were subjected to antiviral screening bioassay to investigate and to evaluate their biological activities. | 2009 | 19666102 |
| super paramagnetic clustering of dna sequences. | an unsupervised clustering of 4541 dna sequences containing active promoter regions from vertebrate and arthropod classes (including their viral genes) was performed. all necessary information was solely gathered a priori from the dna sequences by measuring frequencies of tri-nucleotides and tetra-nucleotides. we employed super paramagnetic clustering, a novel clustering algorithm based on physical properties of an inhomogeneous granular ferromagnet. this method utilizes swendsen-wang cluster mo ... | 2006 | 19669432 |
| proteomic analysis of cells in the early stages of herpes simplex virus type-1 infection reveals widespread changes in the host cell proteome. | during infection by herpes simplex virus type-1 (hsv-1) the host cell undergoes widespread changes in gene expression and morphology in response to viral replication and release. however, relatively little is known about the specific proteome changes that occur during the early stages of hsv-1 replication prior to the global damaging effects of virion maturation and egress. to investigate pathways that may be activated or utilised during the early stages of hsv-1 replication, 2-de and lc-ms/ms w ... | 2009 | 19670248 |
| chromatin assembly on herpes simplex virus genomes during lytic infection. | the human herpes simplex viruses hsv-1 and hsv-2 infect a significant portion of the human population. both viruses can undergo lytic infection in epithelial cells and establish lifelong latency in neuronal cells. the large hsv-1 dna genomes have long been considered to be devoid of histones both inside the virion particle and inside the cell during lytic infection, but to be packaged in repressive chromatin during latency. however, recent reports indicate that many histone and non-histone chrom ... | 2010 | 19682614 |
| human cytomegalovirus productively infects adrenocortical cells and induces an early cortisol response. | following our recent findings on the presence of human cytomegalovirus (hcmv) in the normal human adrenal cortex and in adrenocortical tumors, especially in cortisol-secreting tumors, aim of the present study was to investigate the direct effects of hcmv infection on human adrenocortical cells. to this aim, both clinical isolates and laboratory strains of hcmv were used to assess the early effects of infection on human adrenocortical cell morphology, proliferation, gene expression, and steroidog ... | 2009 | 19688782 |
| herpes simplex virus type 1 production requires a functional escrt-iii complex but is independent of tsg101 and alix expression. | herpes simplex virus type 1 (hsv-1) acquires its mature virus envelope by budding into the lumen of cytoplasmic membranous compartments carrying the viral glycoproteins. in a cellular context, a budding process with identical topology occurs during the formation of intraluminal vesicles in multivesicular bodies. the cellular machinery that mediates this budding process is composed of four protein complexes termed endosomal sorting complexes required for transport (escrts) and several associated ... | 2009 | 19692479 |
| benefits of oat beta-glucan and sucrose feedings on infection and macrophage antiviral resistance following exercise stress. | oat beta-glucan can counteract the exercise-induced increased risk for upper respiratory tract infection (urti) in mice, which is at least partly mediated by its effects on lung macrophages. substantial evidence in humans indicates that carbohydrate-containing sports drinks can offset the decreased immune function associated with stressful exercise. however, no studies in animals or humans have directly examined their effects on urti using a controlled virus-challenge model. we examined the effe ... | 2009 | 19692658 |
| protection from herpes simplex virus (hsv)-2 infection with replication-defective hsv-2 or glycoprotein d2 vaccines in hsv-1-seropositive and hsv-1-seronegative guinea pigs. | a herpes simplex virus (hsv)-2 candidate vaccine consisting of glycoprotein d (gd2) in alum and monophosphoryl lipid a (mpl) reduced genital herpes disease in hsv-1-seronegative women but not in men or hsv-1-seropositive women. | 2009 | 19702506 |
| icp0 enables and monitors the function of d cyclins in herpes simplex virus 1 infected cells. | the herpes simplex virus 1 icp0 is a regulatory protein. early in infection icp0 localizes in nd10 bodies and performs two functions: as an e3 ligase in conjunction with e2 ubch5a conjugating enzyme, it degrades the nd10 components pml and sp100. concurrently, it suppresses the silencing of viral dna by dispersing the hdac1/corest/rest/lsd1 repressor complex. subsequently, icp0 is exported to the cytoplasm. in cells treated with hdac inhibitors or transfected with irrelevant dna, the export is d ... | 2009 | 19706544 |
| viral seeding of a percutaneous endoscopic gastrostomy tract from oral herpes. | 2010 | 19716435 | |
| a novel function of heparan sulfate in the regulation of cell-cell fusion. | despite the important contribution of cell-cell fusion in the development and physiology of eukaryotes, little is known about the mechanisms that regulate this process. our study shows that glycosaminoglycans and more specifically heparan sulfate (hs) expressed on the cell surface and extracellular matrix may act as negative regulator of cell-cell fusion. using herpes simplex virus type-1 as a tool to enhance cell-cell fusion, we demonstrate that the absence of hs expression on the cell surface ... | 2009 | 19726670 |
| trichomoniasis and other sexually transmitted infections: results from the 2001-2004 national health and nutrition examination surveys. | to estimate the association between trichomonas vaginalis infection (tv) and 6 sexually transmitted infections: chlamydia, gonorrhea, herpes simplex virus (types 1 and 2), syphilis, and human immunodeficiency virus (hiv) in a nationally representative sample. | 2009 | 19734826 |
| antiviral activity of rhus aromatica (fragrant sumac) extract against two types of herpes simplex viruses in cell culture. | we report on the antiviral potency of an aqueous extract of root/stem bark of rhus aromatica (fragrant sumac extract) against herpes simplex virus type 1 and type 2 in cell culture (rc-37 cells) using a plaque reduction assay. the extract exhibited a high level of anti-hsv activity with ic50-values of 0.0005% for hsv-1 and 0.0043% for hsv-2 as well as high selectivity indices (si) of 5400 for hsv-1 and 628 for hsv-2. in order to determine the mode of antiviral action, the fragrant sumac extract ... | 2009 | 19746844 |
| in vitro antiherpetic and antirotaviral activities of a sulfate prepared from mimosa scabrella galactomannan. | a chemically sulfated galactomannan (brs) from seeds of mimosa scabrella had in vitro antiviral activity against herpes simplex virus 1 (hsv-1), but not against simian rotavirus a/sa11 (sirv-a/sa11). it was examined by (13)c nmr spectroscopy, which showed the sulfate groups to be mainly at c-6 of galactose residues. brs had a selective inhibition against hsv-1 during its attachment step, having an ic(50) lower than 2.5microg/ml, determined by plaque reduction, and a selectivity index of greater ... | 2009 | 19751759 |