Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| conjunctival papillomas caused by human papillomavirus type 33. | 2002 | 11831924 | |
| use of human papillomavirus dna testing to compare equivocal cervical cytologic interpretations in the united states, scandinavia, and the united kingdom. | human papillomavirus (hpv) dna testing may be useful in clarifying equivocal cervical cytologic interpretations. one application might be to standardize the meaning of equivocal interpretations from laboratories in various regions. because international differences may be particularly marked, international comparisons of emerging data will require clear translations of "equivocal" and similar terms. | 2002 | 11836698 |
| resolution of recurrent perianal condylomata acuminata by topical cidofovir in patients with hiv infection. | anogenital condylomata acuminata are the most frequent clinical manifestation of genital human papillomavirus (hpv) infection. association between human immunodeficiency virus (hiv) and hpv infections is frequent (range: 26-60% in males). topical cidofovir (a nucleotide analogue antiviral drug active against a broad range of dna viruses) is a potential treatment for anogenital warts in immunocompromised patients. we treated three hiv-infected patients with hpv perianal condylomas with topical 1% ... | 2001 | 11843219 |
| high incidence of cervical human papillomavirus infection in women during their first sexual relationship. | the prevalence of cervical human papillomavirus increases with increasing numbers of sexual partners, leaving the impression that this infection is acquired only as a result of high risk sexual behaviour. using longitudinal data from 242 women who had only had one sexual partner, we found that the risk of acquiring cervical human papillomavirus infection was 46% (95% ci 28-64) at three years after first intercourse and that the median time from first intercourse to first detection of human papil ... | 2002 | 11845815 |
| comparative analysis of the intracellular location of the high- and low-risk human papillomavirus oncoproteins. | we have compared the intracellular location of the hpv e6 and e7 proteins from high- and low-risk virus types. while high-risk hpv e7 displays diffuse nuclear expression, low-risk e7 has a nuclear punctuate pattern of expression. similarly, while high-risk e6 is expressed throughout the cell, low-risk e6 is again predominantly nuclear with a punctuate pattern of expression. both low-risk viral oncoproteins show colocalization with pml, whereas high-risk viral proteins do not. finally, inhibition ... | 2002 | 11853395 |
| the role of human papillomaviruses in human cancers. | of the more than 100 different human papillomavirus types (hpvs), the "high-risk" hpvs are associated with the vast majority of cervical carcinoma, and a pathologically distinct group of oropharyngeal tumors. in addition, other hpvs are associated with cutaneous tumors, in particular epidermodysplasia verruciformis and non-melanoma skin cancers. in general, hpv-associated cancers arise from a single accidental integration event of the viral genome into a host cell chromosome. integration is a te ... | 2002 | 11861215 |
| activation of nuclear factor kappab in radioresistance of tp53-inactive human keratinocytes. | many tumors show a mutant or inactive tumor suppressor p53 (tp53) status, and tp53 in the tumor-carrying human papillomavirus (hpv) may be dysfunctional because of inhibition by the viral protein hpv e6. molecular mechanisms underlying radiation responses and the radiation-induced resistant phenotype in the tp53-inactive tumor have not been well investigated. in the present study, using a human keratinocyte line (hk18) with tp53 inhibited by hpv18 infection, we demonstrated that nuclear factor ( ... | 2002 | 11861406 |
| specific inactivation of inhibitory sequences in the 5' end of the human papillomavirus type 16 l1 open reading frame results in production of high levels of l1 protein in human epithelial cells. | the expression of human papillomavirus type 16 late genes encoding virus capsid proteins l1 and l2 is restricted to terminally differentiated epithelial cells in the superficial layers of the squamous epithelium. we wish to understand the molecular mechanisms that determine the levels of expression of the human papillomavirus type 16 late genes. we have previously shown that the l1 coding region contains inhibitory sequences. here we extend previous findings to show that the 5' end of the l1 gen ... | 2002 | 11861841 |
| [research on the oncogenesis of cervical epithelial cells co-induced by human papillomavirus and human cytomegalovirus]. | the carcinogenesis of the human cervical carcinoma is known closely associated with human papillomavirus (hpv). the purpose of this article is to identify whether another sexually transmitted factor, human cytomegalovirus (hcmv) plays as a co-factor role in the carcinogenesis. | 1999 | 11869507 |
| variants of the long control region and the e6 oncogene in european human papillomavirus type 16 isolates: implications for cervical disease. | high-risk human papillomavirus types, especially type 16, are risk factors for cervical cancer. preliminary studies suggest that hpv16 polymorphisms in the long control region or in the e6 gene may alter the oncogenic potential of the virus. this could partially explain why some lesions progress to cancer while others do not. a systematic study combining the long control region and e6 has not been undertaken. this prompted us to investigate the long control region and the e6 in northern european ... | 2002 | 11870518 |
| preventive human papillomavirus vaccination. | 2002 | 11872848 | |
| strategies for the modification of risk factors in gynecological cancers. | strategies to modify risk for female or gynecological cancers will vary with our knowledge of the epidemiology, etiology, and specific molecular mechanisms for each individual cancer. in general, cancer preventive strategies have been divided into primary and secondary prevention with primary prevention directed toward the causative factors for a disease. secondary prevention is classically used in cervical cancer cytology screening programs and is essentially an attempt to identify individuals ... | 2002 | 11876392 |
| detection of human papillomavirus dna by pcr/microfluorometry for screening of cervical cancer. | cervical cancer screening is conducted by a cytological papanicolaou (pap) test. for screening, it is becoming increasingly important to introduce a more objective result, based on human papillomavirus (hpv) dna test. we describe here a practical method allowing the mass detection of hpv-dna by pcr followed by fluorogenic dna intercalation. | 2002 | 11880111 |
| results of human papillomavirus dna testing with the hybrid capture 2 assay are reproducible. | reproducibility of the hybrid capture 2 test (hc 2) for human papillomavirus (hpv) dna detection was evaluated by assaying frozen cervical specimens in 1997 and again in 2001 from 1,775 women with normal cervical cytology. using a cutoff point of 1.0 pg of hpv dna/ml between a negative and a positive test result, the result of the kappa test for agreement was 0.72 (a kappa value of >0.60 is considered good agreement). using cutoff points of 1.0 and 10.0 pg/ml between negative and low positive an ... | 2002 | 11880448 |
| stable replication of papillomavirus genomes in saccharomyces cerevisiae. | papillomaviruses normally replicate in stratified squamous epithelial tissues of their mammalian hosts, in which the viral genome is found as a nuclear plasmid. two viral proteins, e1, a helicase, and e2, a transcriptional activator and plasmid maintenance factor, are known to contribute to the episomal replication of the viral genome. recently, our laboratory discovered that papillomaviruses can also replicate in an e1-independent manner in mammalian cells (k. kim and p. f. lambert, virology, i ... | 2002 | 11884560 |
| enhanced immunogenicity of hpv 16 e7 fusion proteins in dna vaccination. | dna vaccination is a promising approach for inducing both humoral and cellular immune responses. for immunotherapy of hpv-16-associated diseases the e7 protein is considered a prime candidate, as it is expressed in all hpv-16-positive tumors. unfortunately, the e7 protein is a very poor inducer of a cytotoxic t-cell response, when being used as antigen in dna vaccination. here we demonstrate that after fusion to protein export/import signals such as the herpes simplex virus ferry protein vp22, e ... | 2002 | 11886264 |
| parp determines the mode of cell death in skin fibroblasts, but not keratinocytes, exposed to sulfur mustard. | sulfur mustard is cytotoxic to dermal fibroblasts as well as epidermal keratinocytes. we demonstrated that poly(adp-ribose) polymerase (parp) modulates fas-mediated apoptosis, and other groups and we have shown that parp plays a role in the modulation of other types of apoptotic and necrotic cell death. we have now utilized primary dermal fibroblasts, immortalized fibroblasts, and keratinocytes derived from parp(-/-) mice and their wildtype littermates (parp(+/+)) to determine the contribution o ... | 2001 | 11886524 |
| failure to detect human papillomavirus dna in malignant epithelial neoplasms of conjunctiva by polymerase chain reaction. | to elucidate the putative role of human papillomavirus (hpv) infection in the etiology of conjunctival tumors, 44 formalin-fixed, paraffin-embedded specimens of conjunctival tumors (24 patients with papillomas and 20 patients with dysplastic and/or malignant tumors) were screened for hpv infection using 4 different polymerase chain reactions (pcrs). of the 24 samples of papilloma, 14 (58%) displayed positive results by applying nested pcr using primer sets of hpv consensus l1 region. hpv type 6 ... | 2002 | 11888082 |
| abrogation of irf-1 response by high-risk hpv e7 protein in vivo. | we have previously reported that human papillomavirus (hpv) e7 interacts with irf-1, a key regulator of cellular immune response, and abrogates its transactivation function at the molecular level in vitro. to confirm our previous data, we investigated in vivo the e7-mediated down-regulation of irf-1 using hpv e7-inducible cells and transgenic mice expressing hpv-18 e6/e7. when e7 was induced in the absence of tetracycline, the expression of target genes of irf-1 (tap-1, ifn-beta, mcp-1 that are ... | 2002 | 11888675 |
| primary care of hpv management in hiv-infected women. | the natural history of human papillomavirus (hpv) differs in women infected with (human immunodeficiency virus) hiv when compared with the general population. this report provides insight into the complexities of treating hpv infection and the differences found in hiv-infected women. by understanding the challenges associated with this opportunistic infection in hiv-infected women, nurse practitioners will be better prepared to provide primary care to this specific population. | 2001 | 11889689 |
| immortalization of bovine umbilical vein endothelial cells: a model for the study of vascular endothelium. | endothelial cells perform a large array of physiological functions that are influenced by their cellular heterogeneity in the different vascular beds. vein endothelial cells isolated from the umbilical cords are commonly used to study vascular endothelium. primary cultures of these cells, however, have low proliferative capacity and a limited life span. we have immortalized bovine umbilical vein endothelial cells (buvec) by transfection with an expression vector containing the human papillomavir ... | 2002 | 11893074 |
| apoptosis of squamous cells at different stages of carcinogenesis following 4-hpr treatment. | squamous cell carcinoma (scc) is the end product of a multistep process characterized by a progression from normal epithelial cells through metaplastic or dysplastic intraepithelial changes that evolve into invasive cancer. since retinamides have shown promising in vivo anti-tumoral activity, we studied effects and effector mechanisms of the synthetic retinoid n-(4-hydroxyphenyl)retinamide (4-hpr) on squamous cells at progressing stages of tumorigenesis. to this end, an in vitro model of squamou ... | 2002 | 11895859 |
| sodium lauryl sulfate, a microbicide effective against enveloped and nonenveloped viruses. | the number of individuals infected with human immunodeficiency virus (hiv) and other pathogens causing sexually transmitted diseases (stds) is growing dramatically worldwide. globally, heterosexual transmission may account for as much as 85-90% of new cases of hiv infection. latex condoms represent an effective barrier against sexually transmitted pathogens, but unfortunately, their use is not generalized. therefore, there is an urgent need to develop safe and potent topical microbicides under t ... | 2002 | 11899262 |
| immune evasion in human papillomavirus-associated cervical cancer. | tumour-associated viruses produce antigens that, on the face of it, are ideal targets for immunotherapy. unfortunately, these viruses are experts at avoiding or subverting the host immune response. cervical-cancer-associated human papillomavirus (hpv) has a battery of immune-evasion mechanisms at its disposal that could confound attempts at hpv-directed immunotherapy. other virally associated human cancers might prove similarly refractive to immuno-intervention unless we learn how to circumvent ... | 2002 | 11902586 |
| comparison of hpv infection, p53 mutation and allelic losses in post-transplant and non-posttransplant oral squamous cell carcinomas. | oral squamous cell carcinoma (scc) is increasingly found in transplant recipients, although little is known of the natural history of the disease or the mechanism underlying this increase. | 2002 | 11903818 |
| complementation of a p300/cbp defective-binding mutant of adenovirus e1a by human papillomavirus e6 proteins. | previous studies have shown that the human papillomavirus type 16 (hpv-16) e6 protein binds to p300/cbp and abrogates its transcriptional co-activator function. however, there is little information on the biological consequences of this interaction and discrepancy as to whether the interaction is high-risk e6 specific or not. we performed a series of studies to compare the interactions of hpv-18 and hpv-11 e6 with p300, and showed that both high- and low- risk e6 proteins bind p300. in addition, ... | 2002 | 11907332 |
| human papillomavirus, smoking, and cancer. | the effect of smoking on human papillomavirus (hpv) activity and subsequent dysplasia and neoplasia remains controversial. | 2001 | 11907844 |
| [epidemiology and natural history of genital infection by human papillomavirus]. | human papillomavirus genital infection is a very common sexual transmitted disease, probably the most common of them. on one hand, this infection is more often than not transient and asymptomatic and induces an effective immunity which allows the infection cure; on the other hand it can be responsible for an intraepithelial lesion which can progress to an invasive cancer. in spite of the decrease of cervical cancer incidence thanks to pap smear screening, it remains a real preoccupation for clin ... | 2002 | 11910884 |
| [high and low risk human papilloma virus infection in women with cin. differential characteristics]. | the objective of this study was to determine high risk human papillomavirus infection (hpv-rh) and factors with cervical intraepithelial neoplasia appears (cin). | 2002 | 11913045 |
| risk factors of invasive cervical cancer in mali. | cervical cancer is the most common cancer in women in mali and the second commonest cause of cancer mortality. | 2002 | 11914322 |
| co-existent carcinosarcoma and adenoid basal carcinoma of the uterine cervix and correlation with human papillomavirus infection. | a case of carcinosarcoma associated with an adenoid basal carcinoma of the uterine cervix in an 84-year-old woman is described. the tumor formed a pelvic mass, and total hysterectomy with bilateral salpingo-oophorectomy was performed. the tumor was 17 cm in maximal dimension, arose in the posterior wall of the uterine cervix, and had a solid and cystic, focally myxoid sectioned surface. the tumor was composed of keratinizing squamous cell carcinoma and a mostly nonspecific sarcomatous component ... | 2002 | 11917230 |
| the causal relation between human papillomavirus and cervical cancer. | the causal role of human papillomavirus infections in cervical cancer has been documented beyond reasonable doubt. the association is present in virtually all cervical cancer cases worldwide. it is the right time for medical societies and public health regulators to consider this evidence and to define its preventive and clinical implications. a comprehensive review of key studies and results is presented. | 2002 | 11919208 |
| direct visual inspection for cervical cancer screening: an analysis of factors influencing test performance. | the authors evaluated direct visual inspection of the cervix after the application of 5% acetic acid (dvi) as a cervical cancer screening test for use in low-resource settings. | 2002 | 11920531 |
| persistent human papillomavirus infection and smoking increase risk of failure of treatment of cervical intraepithelial neoplasia (cin). | women with abnormal smears have an increased risk of developing cervical cancer. during the 8 years following conservative treatment of cervical intraepithelial neoplasia (cin), their risk of invasive cervical cancer is about 5 times greater than that of the general population. human papillomavirus (hpv) has been associated with the natural history of both cin and cervical cancer. to date, there have been no published reports on the predictive value of hpv testing in the treatment outcome of cin ... | 2002 | 11920596 |
| human papillomavirus 16 load in normal and abnormal cervical scrapes: an indicator of cin ii/iii and viral clearance. | the relation between human papillomavirus type 16 (hpv 16) viral load in cervical scrapes and development of high-grade cervical intraepithelial neoplasia (cin ii or iii) was studied in a nested case-control study of women with normal cytology (group a) and in a cohort of women with abnormal cytology (group b). hpv 16 dna load was determined using a quantitative real-time pcr assay. in group a, case women (women with cin ii/iii, n = 12) had a significantly higher viral load than control women (w ... | 2002 | 11920620 |
| epigenetics of breast cancer: polycyclic aromatic hydrocarbons as risk factors. | in the absence of a causal relationship between the incidence of sporadic breast cancer and occurrence of mutations in breast cancer susceptibility genes, efforts directed to investigating the contribution of environmental xenobiotics in the etiology of sporadic mammary neoplasia are warranted. polycyclic aromatic hydrocarbons (pahs) are ubiquitous pollutants, which have been shown to induce dna damage and disrupt cell cycle progression. in this report we discuss published data pointing to pahs ... | 2002 | 11921194 |
| senescence induced by altered telomere state, not telomere loss. | primary human cells in culture invariably stop dividing and enter a state of growth arrest called replicative senescence. this transition is induced by programmed telomere shortening, but the underlying mechanisms are unclear. here, we report that overexpression of trf2, a telomeric dna binding protein, increased the rate of telomere shortening in primary cells without accelerating senescence. trf2 reduced the senescence setpoint, defined as telomere length at senescence, from 7 to 4 kilobases. ... | 2002 | 11923537 |
| antiproliferative and apoptotic effects of iron chelators on human cervical carcinoma cells. | cervical carcinoma is a human papillomavirus (hpv)-associated cancer for which treatment options still mainly rely on surgical procedures, with or without adjuvant radiotherapy and chemotherapy. as iron may participate in the pathogenesis of viral infections and cancer in several ways, the present study was designed to investigate the effect of iron chelation on hpv-16- and hpv-18-positive cervical carcinoma cell lines. | 2002 | 11925126 |
| human papillomavirus persistence and nutrients involved in the methylation pathway among a cohort of young women. | persistent oncogenic human papillomavirus (hpv) infection is associated with cervical dysplasia. cofactors, such as nutrient status, may be required for the progression of hpv infection to neoplasia. hpv dna methylation patterns in vitro have been shown to be associated with viral transcriptional activity. folate, vitamin b12, vitamin b6, and methionine may function to prevent cervical cancer through their role in dna methylation. this study was conducted to examine the relationship of dietary i ... | 2002 | 11927495 |
| treatment of sexually transmitted infections with single-dose therapy: a double-edged sword. | since the advent of the antimicrobial era, single-dose therapy has been a valuable tool in the management of genital infection. most of the common sexually transmitted infections (stis) such as gonorrhoea, syphilis, trichomoniasis and chancroid can be treated in this way, as can genital infections which are not sexually transmitted such as bacterial vaginosis and genital tract candidiasis. until recently, treatment for chlamydia trachomatis infection required a multi-dose regimen, but single-dos ... | 2002 | 11929335 |
| multichannel piezoelectric genesensor for the detection of human papilloma virus. | to establish a method for rapid detection and sub-typing of human papilloma virus (hpv). | 2002 | 11940384 |
| papillary squamous cell carcinoma of the uterine cervix: a report of two cases with human papillomavirus 16 dna. | 2002 | 11942912 | |
| [treatment of dystrophy, dysplasia and initial stages of vulvar carcinoma in virus infections]. | overall sixty-seven patients with distrophy, dysplasia and valvular carcinoma presenting with human papilloma virus- and herpes simplex-2 virus infections underwent a complex treatment intended for background, precancerous affections of the vulva and for valvular carcinoma, involving the use of antiviral drug preparations laferon, zovirax, lovir (both on the systemic and local basis), and an immunomodulator (decaris). efficacy of the above treatment option has been shown to be 98.5%. | 2002 | 11944391 |
| squamous cell carcinoma of the tonsils: a molecular analysis of hpv associations. | the role of human papilloma virus (hpv) in the pathogenesis and biological behavior of tonsillar squamous cell carcinoma (tscc) are areas of intense investigation. | 2002 | 11948119 |
| epidermodysplasia verruciformis associated with neurofibromatosis type 1: coincidental association or model for understanding the underlying mechanism of the disease? | we describe a 25-year-old man with epidermodysplasia verruciformis (ev) associated with neurofibromatosis type 1 (nf1). the lesions, persisting for more than 15 years, consisted of widespread planar warts on the backs of the hands and wrists, and reddish-brown macules on the trunk, neck and face. during the last 5 years, our patient developed several epithelial tumours, namely solar keratoses, plaques of bowen's disease and squamous cell carcinomas (sccs). he also presented with nf1 lesions with ... | 2002 | 11952554 |
| [establishment of human immortalized oral epithelial cell line hio615 induced by hpv16 e6 and e7]. | to establish an immortalized oral epithelial cell line. | 2002 | 11955351 |
| 2001 consensus guidelines for the management of women with cervical cytological abnormalities. | to provide evidence-based consensus guidelines for the management of women with cervical cytological abnormalities and cervical cancer precursors. | 2002 | 11966387 |
| antibodies to human papillomavirus 16 l1 virus-like particles as an independent prognostic marker in cervical cancer. | infection with high-risk human papillomavirus (hpv) types such as hpv-16 is a major risk factor for the development of cervical cancer. hpv-16 capsid antibodies are detectable in approximately 50% of patients with hpv-16 dna-positive cervical cancer. we investigated the prognostic significance of hpv capsid antibodies for survival in patients with cervical cancer in comparison with conventional clinicopathologic features such as staging, histologic grading, histology, age, and treatment modality ... | 2002 | 11967495 |
| an atpase assay using scintillation proximity beads for high-throughput screening or kinetic analysis. | a new procedure for measuring atpase activity in which gamma-(33)p-labeled inorganic orthophoshate is detected by addition of ammonium molybdate followed by selective adsorption of the resulting phosphomolybdate to scintillation proximity beads in the presence of cesium chloride is described. this method is shown to give accurate and reproducible results over a wide range of detection conditions and product concentrations. it requires no separation or filtration steps and is highly compatible wi ... | 2002 | 11969189 |
| nuclear import strategies of high risk hpv16 l1 major capsid protein. | during the late phase of human papillomavirus (hpv) infection, the l1 major capsid proteins enter the nuclei of host epithelial cells and, together with the l2 minor capsid proteins, assemble the replicated viral dna into virions. we investigated the nuclear import of the l1 major capsid protein of high risk hpv16. when digitonin-permeabilized hela cells were incubated with hpv16 l1 capsomeres, the l1 protein was imported into the nucleus in a receptor-mediated manner. hpv16 l1 capsomeres formed ... | 2002 | 11971900 |
| two cases of coincident carcinomas of the head and neck and the uterine cervix. | human papillomavirus (hpv) is an independent risk factor for select head and neck carcinomas and most uterine cervix carcinomas. we report two patients with synchronous diagnoses of cervical cancer and hpv-related head and neck cancer. | 2002 | 11972404 |
| multiple bowen's disease of the fingers. | we describe herein a case of multiple bowen's disease that developed on the left hand fingers of an 80-year-old male patient who had practiced as a gynecologist. pcr-based analysis indicated that the lesion contained human papillomavirus (hpv) type 18 dna. topical application of bleomycin and liquid nitrogen cryotherapy were effective in treating this case. after treatment, histopathologically no atypical cells were seen throughout the epidermis. | 2002 | 11978572 |
| human papillomavirus-negative spindle cell carcinoma of the vulva associated with lichen sclerosus: case report and literature review. | although spindle cell carcinoma (sc) is a common neoplasm in the oral cavity, upper respiratory tract, and other head and neck areas, its occurrence in the vulva is rare. we report a case of this rare condition with immunohistochemical, ultrastructural, and human papillomavirus (hpv) testing. the neoplastic cells were positive for vimentin, keratins (ae1-ae3, keratin 902, and keratin 903), and epithelial membrane antigen. ultrastructurally, they showed primitive junctions and tonofilaments. hpv ... | 2002 | 11979073 |
| human papillomavirus antigens and t-cell recognition. | this review will summarize what is known about natural t-cell responses to human papillomavirus infections, including potential mechanisms for their dysregulation which may lead to the development of disease. we will also describe new strategies to enhance human papillomavirus specific t-cell responses following vaccination that are currently in development and recent reports on human vaccine trials with particular regard to the generation of appropriate t-cell responses. | 2001 | 11979123 |
| optimization of a human papillomavirus-specific enzyme-linked immunosorbent assay. | a strategy was developed for the control, standardization, and critical evaluation of an enzyme-linked immunosorbent assay (elisa) for the detection of human papillomavirus-specific immunoglobulin g in human sera. control human sera, polyclonal animal sera, and monoclonal antibodies were used to establish optimal assay parameters, including antigen coating, serum dilutions, and criteria for daily reproducibility, monitoring, and rejection of assays. three evaluation techniques were used in paral ... | 2002 | 11986263 |
| the human papillomavirus oncoprotein e7 attenuates nf-kappa b activation by targeting the ikappa b kinase complex. | infection with high-risk human papillomaviruses (hpv) can lead to the development of cervical carcinomas. this process critically depends on the virus-encoded e6 and e7 oncoproteins, which stimulate proliferation by manipulating the function of a variety of host key regulatory proteins. here we show that both viral proteins dose-dependently interfere with the transcriptional activity of nf-kappab. a variety of experimental approaches revealed that a fraction of the e7 proteins is found in associ ... | 2002 | 11986318 |
| [detection of hpv16 e6 gene in cervical tissues by quantitative polymerase chain reaction]. | to study the relationship between the quantities of hpv16 e6 gene in cervical tissues and the course of cervical disease. | 2001 | 11986698 |
| cost-effectiveness of alternative triage strategies for atypical squamous cells of undetermined significance. | every year approximately 2 million us women are diagnosed as having a cervical cytological result of atypical squamous cells of undetermined significance (asc-us). | 2002 | 11988059 |
| the acceptability of self-collected samples for hpv testing vs. the pap test as alternatives in cervical cancer screening. | to explore the acceptability of the self-collection of samples for human papillomavirus (hpv) testing in comparison with that of the pap test. | 2002 | 11988136 |
| ins and outs of clinical trials with peptide-based vaccines. | peptides are the smallest antigenic components that are recognized by t cells when presented in mhc molecules on the cell surface. after the identification of peptides from tumor associated and tumor specific antigens, the exploration of the use of peptides in immunotherapy of cancer was instigated. from initial exploration of peptide-mediated induction of immune responses in mice, the peptide based vaccines have evolved to clinical testing in cancer patients. many different clinical trials have ... | 2002 | 11991853 |
| single-cell cytokine analysis allows detection of cervical t-cell responses against human papillomavirus type 16 l1 in women infected with genital hpv. | specific types of human papillomavirus (hpv) are known to play a causal role in the development of cervical cancer, with human papillomavirus type 16 (hpv-16) identified as the predominant type. despite this, little is known about cervical immune responses to this pathogen. the aim of this study was to assess the feasibility of cervical cytobrush sampling and single-cell cytokine staining to investigate cervical lymphocyte-specific cytokine responses to hpv-16 antigens. of eighteen women recruit ... | 2002 | 11992584 |
| local treatment of hpv-induced skin lesions by cidofovir. | human papillomavirus (hpv) induced epithelial cell proliferation is responsible for a broad range of lesions. treatment of such lesions is characterized by a high relapse rate. four patients are described who were treated locally with cidofovir (cream 1% or injection of 2.5 mg/ml solution) for multi-treated relapsing hpv-associated lesions. three of the four patients had a complete response. the fourth patient showed a clinical response in the first cycle of treatment, but new lesions appeared d ... | 2002 | 11992585 |
| observer variation, dysplasia grading, and hpv typing: a review. | squamous dysplasia of the cervix is a morphologic continuum that is divided into a number of categories. when the severity of a morphologic abnormality is assessed, whether in a biopsy sample or in an exfoliative smear, there can be significant observer variation. the statistical quantification of this variation, with particular reference to the kappa statistic and the influence of the number of categories utilized on the kappa statistic, is discussed. the contribution of information theory to t ... | 2000 | 11996166 |
| an explosive course of squamous cell penile cancer in an aids patient. | we report the case of a 37-year-old man in whom penile cancer was discovered while he was treated for aids 4 years after a human papillomavirus (hsv) infection. despite initially localised disease with t1 n0 staging, he died of metastasis within 3 years. a brief review of the literature regarding hpv-related cancer in hiv-infected patients is presented and therapeutic options are discussed. | 2002 | 11996481 |
| the alkaloid sanguinarine is effective against multidrug resistance in human cervical cells via bimodal cell death. | sanguinarine, a benzophenanthrine alkaloid, is potentially antineoplastic through induction of cell death pathways. the development of multidrug resistance (mdr) is a major obstacle to the success of chemotherapeutic agents. the aim of this study was to investigate whether sanguinarine is effective against uterine cervical mdr and, if so, by which mechanism. the effects of treatment with sanguinarine on human papillomavirus (hpv) type 16-immortalized endocervical cells and their mdr counterpart ... | 2002 | 11996882 |
| protean manifestations of human papillomavirus type 60 infection on the extremities. | human papillomavirus type 60 (hpv-60) induces a ridged wart or an epidermal cyst on the sole of the foot, exhibiting identical pathological changes, with a single refractile eosinophilic intracytoplasmic inclusion body in infected cells. however, there is no information on the role of hpv-60 in the development of cutaneous lesions on other anatomical sites. | 2002 | 12000390 |
| human papillomavirus and blastocyst apoptosis. | the effect of human papillomavirus (hpv) dna from the e6-e7 region on the integrity of dna in blastocyst stage embryonic cells was studied. the study design paralleled the event whereby hpv dna from the infecting virus would target host cell dna. the objectives were (a) to determine if the dna of blastocysts were disrupted by the presence of hpv dna and (b) to determine if the intensity of dna damage was associated with the type of hpv. | 2002 | 12005308 |
| real-time quantitative pcr demonstrates low prevalence of human papillomavirus type 16 in premalignant and malignant lesions of the oral cavity. | human papillomavirus (hpv) type-16 has been associated with invasive squamous cell carcinoma of the head and neck. this study examines the role of hpv-16 in the progression of oral head and neck cancer by determining the quantity of hpv-16 dna in premalignant and malignant lesions, using real-time quantitative pcr, to more accurately determine the role of hpv-16 in oral head and neck squamous cell carcinogenesis. | 2002 | 12006539 |
| characterization of novel cell lines established from three human oral squamous cell carcinomas. | human oral squamous cell carcinoma cell lines (koscc-11, -25a, -25b, -25c, -25d, -25e, -33a, and -33b) were established by explantation culture from these oral squamous cell carcinomas. the histopathology of the primary tumors, in vitro growth characteristics, epithelial origin, in vitro anchorage-independency, in vivo tumorigenicity, the frequency of human papillomavirus (hpv) infections, and the status of proto-oncogenes, tumor suppressor genes, dna mismatch repair genes, and microsatellite in ... | 2002 | 12011992 |
| [malignant transformation of the immortalized esophageal epithelial cells]. | immortal cell line of human embryonic esophageal epithelium (shee) was induced by e6e7 genes of human papillomavirus (hpv) type 18 in our laboratory. to identify the fully malignant transformation at its 85th passage (shee85), the malignant phenotype, tumorigenesis and invasive potency were studied. | 2002 | 12015026 |
| the human papillomavirus type 31 late 3' untranslated region contains a complex bipartite negative regulatory element. | the papillomavirus life cycle is tightly linked to epithelial cell differentiation. production of virus capsid proteins is restricted to the most terminally differentiated keratinocytes in the upper layers of the epithelium. however, mrnas encoding the capsid proteins can be detected in less-differentiated cells, suggesting that late gene expression is controlled posttranscriptionally. short sequence elements (less than 80 nucleotides in length) that inhibit gene expression in undifferentiated e ... | 2002 | 12021332 |
| reorganization of nuclear domain 10 induced by papillomavirus capsid protein l2. | nuclear domains (nd) 10 are associated with proteins implicated in transcriptional regulation, growth suppression, and apoptosis. we now show that the minor capsid protein l2 of human papillomavirus (hpv) type 33 induces a reorganization of nd10-associated proteins. whereas the promyelocytic leukemia protein, the major structural component of nd10, was unaffected by l2, sp100 was released from nd10 upon l2 expression. the total cellular amount of sp100, but not of sp100 mrna, decreased significa ... | 2002 | 12033769 |
| dna binding of l1 is required for human papillomavirus morphogenesis in vivo. | the role of putative dna-binding domains of human papillomavirus (hpv) capsid proteins for dna encapsidation in vivo is still unknown. we have now analyzed mutants of the major capsid protein l1 of hpv type 33, which are defective for dna binding, for their ability to encapsidate dna using an in vivo packaging approach. since the dna-binding domain and the nuclear localization signal (nls) of l1 overlap, both a carboxy-terminal deletion mutant (l1-1/470) and a substitution mutant (l1-1/477m9) we ... | 2002 | 12033775 |
| susceptibility locus for epidermodysplasia verruciformis not linked to cervical cancer in situ. | cervical cancer is strongly associated with infection by oncogenic forms of human papillomavirus (hpv), mainly hpv 16 and hpv 18. the aim of this study was to test if a locus previously mapped to a region on chromosome 17 qter in patients with epidermodysplasia verucciformis (ev) and psoriasis and considered to be responsible for an increased susceptibility to hpv 5, also is linked to increased hpv susceptibility in cervical cancer in situ. we also wanted to test whether hpv 16 positivity cluste ... | 2001 | 12035615 |
| human papillomavirus e6-induced degradation of e6tp1 is mediated by e6ap ubiquitin ligase. | high-risk human papilloma viruses are known to be associated with cervical cancers. we have reported previously that the high-risk human papillomavirus (hpv) e6 oncoprotein interacts with e6tp1, a novel rap gtpase-activating protein (rapgap). similar to p53 tumor suppressor protein, the high-risk hpv e6 oncoproteins target e6tp1 for degradation. the hpv16 e6-induced degradation of e6tp1 strongly correlates with its ability to immortalize human mammary epithelial cells. in this study, we used tre ... | 2002 | 12036950 |
| efficacy of imiquimod 5% cream in the treatment of recalcitrant warts in children. | long-lasting cutaneous warts are a therapeutic challenge, especially widespread or symptomatic recalcitrant warts in children. it can be speculated that natural immunity to these human papillomavirus (hpv)- induced lesions is extremely poor. therefore ideally treatment should focus on increasing local immune response. recently imiquimod, a topical immune modifier, has been successfully used in the treatment of external genital warts. our purpose is to report on our experiences with imiquimod 5% ... | 2002 | 12047649 |
| intracutaneous dna vaccination with the e8 gene of cottontail rabbit papillomavirus induces protective immunity against virus challenge in rabbits. | the cottontail rabbit papillomavirus (crpv)-rabbit model has been used in several studies for testing prophylactic and therapeutic papillomavirus vaccines. earlier observations had shown that the crpv nonstructural genes e1, e2, and e6 induced strong to partial protective immunity against crpv infection. in this study, we found that crpv e8 immunization eliminated virus-induced papillomas in eiii/jc inbred rabbits (100%) and provided partial protection (55%) against virus challenge in outbred ne ... | 2002 | 12050357 |
| identification of two cross-neutralizing linear epitopes within the l1 major capsid protein of human papillomaviruses. | the neutralizing activities of polyclonal antibodies and monoclonal antibodies (mabs) obtained by immunization of mice with l1 virus-like particles (vlps) were investigated by using pseudovirion infectivity assays for human papillomavirus type 16 (hpv-16), hpv-31, hpv-33, hpv-45, hpv-58, and hpv-59 to obtain a better definition of cross-neutralization between high-risk hpvs. in this study, we confirmed and extended previous studies indicating that most genital hpv genotypes represent separate se ... | 2002 | 12050360 |
| differential effects of human papillomavirus dna types on p53 tumor-suppressor gene apoptosis in sperm. | sperm dna undergoes apoptotic fragmentation when exposed to hpv dna. details of the specific gene regions targeted by hpv in sperm are lacking. the objective of this study was to determine the integrity of exons 5 and 8 of the p53 gene in sperm exposed to hpv dna. | 2002 | 12051883 |
| liquid-based papanicolaou smears without a transformation zone component: should clinicians worry? | to evaluate whether thinprep smears without versus those with a transformation zone component were more likely to have falsely negative cytology findings. | 2002 | 12052599 |
| down-regulation of gata-3 expression during human papillomavirus-mediated immortalization and cervical carcinogenesis. | to identify cellular genes that may be involved in human papillomavirus (hpv)-mediated immortalization mrna differential display analysis was performed on preimmortal and subsequent immortal stages of four human keratinocyte cell lines transformed by hpv type 16 or 18 dna. this yielded a cdna fragment encoding the transcription factor gata-3 that was strongly reduced in intensity in all immortal stages of the four cell lines. a marked reduction in both gata-3 mrna and protein expression in hpv-i ... | 2002 | 12057898 |
| establishment of two immortalized nasopharyngeal epithelial cell lines using sv40 large t and hpv16e6/e7 viral oncogenes. | nasopharyngeal carcinoma (npc) is a common cancer in southeast asia, especially in southern china. one of the most striking features of this disease is its close relationship with epstein-barr virus (ebv). however, to date there is no direct study on the mechanisms involved in the role of ebv in the tumorigenesis of npc, largely due to lack of an experimental model. available hypotheses on the association between ebv and npc are generated from non-nasopharyngeal epithelial cell systems such as h ... | 2002 | 12063178 |
| [human papilloma virus (hpv) infection and its relationship with central intra-epithelial neoplasia (cin)]. | hpv (human papillomavirus) infection is strongly associated and is the main cause of cervical cancer. the main target for hpv viruses is the cervical transformation zone. in our work infection with hpv 18 and hpv 16 as also other types of viruses were investigated in 15 patients by taking biopsies. before hand the patients were signalized by cytology as well as all of them were colposcopically examined. the samples were examined immunohistochemically in the laboratory at the specialized hospital ... | 2002 | 12066550 |
| condyloma acuminatum presenting as a dorsal tongue lesion in a patient with aids. | oral lesions have been recognized as a prominent feature of hiv infection and aids since the beginning of the epidemic. this report describes the case of a man with advanced aids and a nonpainful but enlarging dorsal tongue soft tissue growth of 6 months' duration. incisional biopsy showed a red, papillary lesion with koilocytosis consistent with condyloma acuminatum. in situ hybridization and molecular techniques were used to identify human papillomavirus (hpv)-31 sequences in warty tissue. eig ... | 2002 | 12071187 |
| translational regulation of human papillomavirus type 16 e7 mrna by the peptide seqika, shared by rabbit alpha(1)-globin and human cytokeratin 7. | the possible biochemical factors able to affect the in vitro expression of the high-risk human papillomavirus type 16 (hpv16) e7 oncoprotein have been analyzed. evidence is provided that e7 mrna stability is increased and, conversely, transcript translation is inhibited by binding to a 32-kda protein from rabbit reticulocyte lysate; sequence analysis identified the 32-kda binding protein as rabbit alpha(1)-globin protein; and interaction between rabbit alpha(1)-globin and e7 mrna occurs through ... | 2002 | 12072504 |
| the pathology and molecular biology of anal intraepithelial neoplasia: comparisons with cervical and vulvar intraepithelial carcinoma. | anal intraepithelial neoplasia (ain) is a well-described pathological precursor of invasive squamous cell carcinoma which has recently been detected with increasing frequency in immunocompromised patients, particularly those with seropositivity for human immunodeficiency virus (hiv). the epidemiology and natural history of this entity is somewhat unclear, since the overall prevalence in the hiv seronegative population is unknown. | 2002 | 12073068 |
| established human papillomavirus type 16-expressing tumors are effectively eradicated following vaccination with long peptides. | peptide-based vaccines aimed at the induction of effective t cell responses against established cancers have so far only met with limited clinical success and clearly need to be improved. in a preclinical model of human papillomavirus (hpv)16-induced cervical cancer we show that prime-boost vaccinations with the hpv16-derived 35 amino-acid long peptide e7(43-77), containing both a ctl epitope and a th epitope, resulted in the induction of far more robust e7-specific cd8(+) t cell responses than ... | 2002 | 12077264 |
| different osteochondral potential of clonal cell lines derived from adult human trabecular bone. | cells derived from human trabecular bones have been shown to have multipotential differentiation ability along osteogenic, chondrogenic, and adipogenic lineages. in this study, we have derived two clonal sublines of human trabecular bone cells by means of stable transduction with human papilloma virus e6/e7 genes. our results showed that these clonal sublines differ in their osteochondral potential, but are equally adipogenic, indicative of the heterogeneous nature of the parental cell populatio ... | 2002 | 12081870 |
| induction of senescence-like state and suppression of telomerase activity through inhibition of hpv e6/e7 gene expression in cells immortalized by hpv16 dna. | the e6 and e7 oncoproteins of human papillomavirus (hpv) play a major role in the development of cervical carcinoma. in this study, a recombinant adenovirus that expresses the bovine papillomavirus (bpv) e2, which has been shown to inhibit hpv early gene expression, was delivered to two hpv-immortalized cell lines as well as caski, a cervical carcinoma cell line. we tested whether the carcinoma and the immortal cells were equally affected by the expression of bpv e2. in all cell lines, bpv e2-me ... | 2002 | 12083799 |
| papillomavirus e1 proteins: form, function, and features. | the e1 proteins are the essential origin recognition proteins for papillomavirus (pv) replication. e1 proteins bind to specific dna elements in the viral origin of replication and assemble into hexameric helicases with the aid of a second viral protein, e2. the resultant helicase complex initiates origin dna unwinding to provide the template for subsequent syntheses of progeny dna. in addition to atp-dependent helicase activity, e1 proteins interact with and recruit several host cell replication ... | 2002 | 12086149 |
| analysis of cervico-vaginal (papanicolaou) smears, in girls 18 years and under. | this study was conducted retrospectively at the queen elizabeth hospital and a private laboratory in barbados to determine the types of epithelial abnormalities in cervico-vaginal papanicolaou (pap)-stained smears, and their clinical implications in barbadian girls, 18 years and under, during the five-year period january 1995 to december 1999. two hundred and sixty-five pap smears from 236 patients were examined and the gynaecological history, initial and repeat pap smear diagnoses, and histolog ... | 2002 | 12089874 |
| infrequent occurrence of high-risk human papillomavirus and of p53 mutation in minimal deviation adenocarcinoma of the cervix. | to assess the occurrence of high-risk human papillomavirus (hpv) infection and p53 alterations in minimal deviation adenocarcinoma (mda) of the uterine cervix, paraffin sections were used to investigate the presence of hpvs 16 and 18 and p53 expression and mutation in six cases of mda. by polymerase chain reaction, only one case was positive for hpv 16 and none was positive for hpv 18. by in situ polymerase chain reaction in the case positive for hpv 16, hpv 16 was detected in a low-grade squamo ... | 1999 | 12090589 |
| time course of humoral and cell-mediated immune responses to human papillomavirus type 16 in infected women. | the time course of cell-mediated and humoral immune responses was elucidated in eight women with human papillomavirus type 16 (hpv-16) infection by performing serial hpv-16 e6 and e7 cytotoxic t-lymphocyte (ctl) assays and hpv-16 virus-like particle (vlp) antibody analyses. four subjects had a single incident of hpv-16 dna detection, and four subjects had two periods of hpv-16 dna detection. in two of the women in the latter group, the second episode of hpv-16 detection occurred in the presence ... | 2002 | 12093689 |
| localized distribution of human papillomavirus genotypes in the uterine cervix. | the localization and distribution of single or multiple hpv genotypes in the uterine cervix has not been studied thus far. the present study was undertaken to determine whether single or multiple hpv genotypes detected in cervical smears originate from a single (dysplastic) area, or from different areas (dysplastic or normal) of the uterine cervix. | 2002 | 12094955 |
| treatment of non-genital warts with topical imiquimod 5% cream. | common warts (verrucae vulgaris) are associated with human papillomavirus infection and are routinely treated by ablative procedures such as cryotherapy, electrodessiccation and salicylic acid. we report 10 cases of recurrent warts treated with a potential new topical therapy, imiquimod 5% cream. nine of the 10 patients were successfully treated with imiquimod 5% cream applied, under occlusion, once daily for 4 weeks. no recurrences were reported during 3 months of follow up. | 2002 | 12095879 |
| plant-derived human papillomavirus 16 e7 oncoprotein induces immune response and specific tumor protection. | vaccine strategies for treatment of human papillomavirus-induced cervical cancer are based on either the recombinant e7 fusion oncoprotein or e7 ctl peptides. the therapeutic potential of the e7-based vaccine depends on the use of different adjuvants. in this study, we describe for the first time the expression of the human papillomavirus 16 e7 protein in nicotiana benthamiana plant using a potato virus x-derived vector. c57bl/6 mice immunized with e7-containing crude foliar extracts developed b ... | 2002 | 12097270 |
| analysis of cd4(+) t-cell responses to human papillomavirus (hpv) type 11 l1 in healthy adults reveals a high degree of responsiveness and cross-reactivity with other hpv types. | human papillomavirus type 11 (hpv-11) infection causes genital warts and recurrent respiratory papillomatosis. while there is compelling evidence that cd4(+) t cells play an important role in immune surveillance of hpv-associated diseases, little is known about human cd4(+) t-cell recognition of hpv-11. we have investigated the cd4(+) t-cell responses of 25 unrelated healthy donors to hpv-11 l1 virus-like particles (vlp). cd4(+) t-cell lines from 21 of 25 donors were established. cell sorting ex ... | 2002 | 12097554 |
| overexpression of p16 and p14arf is associated with human papillomavirus infection in cervical squamous cell carcinoma and dysplasia. | the cdkn2 gene encodes two structurally different proteins: a cyclin-dependent kinase inhibitor, p16, which regulates retinoblastoma protein (prb)-dependent g1 arrest, and a cell cycle inhibitor, p14arf, which blocks mdm2-induced p53 degradation resulting in an increase in p53 levels that leads to cell cycle arrest. recent studies have revealed that expression of p16 and p14arf is influenced markedly by the status of prb and p53, and p16 overexpression has been demonstrated in cervical neoplasia ... | 2002 | 12100520 |
| [buschke-lowenstein penile tumor]. | the buschke-löwenstein genital tumour is a poorly defined, uncommon tumour. the distinction between benign lesions, potentially malignant lesions and carcinomatous lesions is difficult. the authors report a case of human papillomavirus (hpv) 11-associated buschke-löwenstein tumour with an area of micro-invasive carcinoma on histological examination of the surgical resection specimen. a 34-year-old patient was operated for recurrent condylomatous lesions of the penis with scrotal extension. histo ... | 2002 | 12108356 |
| human papillomavirus infection and cyclin d1 gene amplification in laryngeal squamous cell carcinoma: biologic function and clinical significance. | human papillomavirus (hpv) infection is suspected to be a risk factor for head and neck, and in particular for laryngeal, carcinogenesis. cyclin d1 gene (ccnd1) overexpression and amplification have been shown to play a role as prognostic factors in many human cancers, among which are head and neck cancers. | 2002 | 12112558 |