Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| activation of murine lymphocytes by exogenous phosphatidylethanolamine- and phosphatidylcholine-specific phospholipase c. | enzymes of the protein kinase c (pkc) family have a decisive role in the activation process of t cells. under physiological conditions, pkc undergoes activation by diacylglycerol (dag) which is transiently produced following activation of an antigen receptor-coupled phosphatidylinositol 4,5-bisphosphate (pip2)-specific phospholipase c (plc) and hydrolysis of pip2. in vitro activation of distinct pkc isoenzymes is differentially regulated by various synthetic dags and membrane phospholipids. we t ... | 1993 | 8242774 |
| partial isolation and some properties of enterotoxin produced by bacillus cereus strains. | extracellular proteins produced by bacillus cereus al-42 and al-15 were fractioned by chromatography on qae-sephadex and sephadex g75. this last chromatographic process resulted in three peaks. the major peak showed vascular permeability activity to rabbits, lethality to mice, and cytotoxicity to vero and hela cells. the analysis by sds-page after ultrafiltration confirm recent findings that the enterotoxin is a compound with molecular mass > 30,000. | 1993 | 8246747 |
| properties of bacillus cereus spores in reference materials prepared from artificially contaminated spray dried milk. | a reference material for bacillus cereus was developed based on spray drying of milk artificially contaminated with b. cereus spores. various properties of the b. cereus spores in the milk powder were determined. the stability of the materials was good with no detectable decrease in the contamination level during 1 1/2 years storage at -20 degrees c or 4 weeks at 22, 30 or 37 degrees c. the homogeneity of the material was found acceptable for use as a reference material. heat treatments (10 min ... | 1993 | 8251303 |
| detection, identification and characterization of bacteriocin-producing lactic acid bacteria from retail food products. | forty bacteriocin-producing (bac+) lactic acid bacteria (lab) were isolated from food samples purchased from retail supermarkets and local farms. of the 40 bac+ isolates, 18 were isolated from 85 food samples by enrichment (21% isolation rate) whereas eight were obtained from 63 samples by direct plating (13% isolation rate). by direct plating, bac+ lab were detected at levels up to 2.4 x 10(5) cfu/g in ready-to-eat meats. the bac+ isolates were identified by carbohydrate fermentation patterns, ... | 1993 | 8257654 |
| a prospective microbiologic surveillance program to detect and prevent the transfusion of bacterially contaminated platelets. | after two patients received bacterially contaminated platelet transfusions, a prospective surveillance program was instituted to perform gram staining and microbiologic culturing of platelets at the time of transfusion. in 12 months, 3141 random-donor platelet pools (prepared from 14,481 units) and 2476 single-donor apheresis units were cultured. all single-donor apheresis units were sterile, but 6 (0.19%) of the random-donor pools were found to be bacterially contaminated, with 1 unit of 5 in t ... | 1993 | 8259595 |
| nih 3t3 cells stably transfected with the gene encoding phosphatidylcholine-hydrolyzing phospholipase c from bacillus cereus acquire a transformed phenotype. | in order to determine whether chronic elevation of intracellular diacylglycerol levels generated by hydrolysis of phosphatidylcholine (pc) by pc-hydrolyzing phospholipase c (pc-plc) is oncogenic, we generated stable transfectants of nih 3t3 cells expressing the gene encoding pc-plc from bacillus cereus. we found that constitutive expression of this gene (plc) led to transformation of nih 3t3 cells as evidenced by anchorage-independent growth in soft agar, formation of transformed foci in tissue ... | 1994 | 8264633 |
| bacillus cereus and related species. | bacillus cereus is a gram-positive aerobic or facultatively anaerobic spore-forming rod. it is a cause of food poisoning, which is frequently associated with the consumption of rice-based dishes. the organism produces an emetic or diarrheal syndrome induced by an emetic toxin and enterotoxin, respectively. other toxins are produced during growth, including phospholipases, proteases, and hemolysins, one of which, cereolysin, is a thiol-activated hemolysin. these toxins may contribute to the patho ... | 1993 | 8269390 |
| an infant with ulcerative colitis complicated by endocarditis and cerebral infarction. | 1993 | 8271136 | |
| antitumor activities of a cephalosporin prodrug in combination with monoclonal antibody-beta-lactamase conjugates. | 7-(phenylacetamido)cephalosporin mustard (cm) and 7-(4-carboxybutanamido)cephalosporin mustard (ccm) were developed as anticancer prodrugs that could be activated site selectively by monoclonal antibody-beta-lactamase conjugates targeted to antigens present on tumor cell surfaces. both cm and ccm were hydrolyzed by purified beta-lactamases from escherichia coli (ec beta l), bacillus cereus (bc beta l), and enterobacter cloacae (ecl beta l). this resulted in the release of phenylenediamine mustar ... | 1993 | 8274516 |
| activation of actin polymerization by phosphatidic acid derived from phosphatidylcholine in iic9 fibroblasts. | alpha-thrombin induced a change in the cell morphology of iic9 fibroblasts from a semiround to an elongated form, accompanied by an increase in stress fibers. incubation of the cells with phospholipase d (pld) from streptomyces chromofuscus and exogenous phosphatidic acid (pa) caused similar morphological changes, whereas platelet-derived growth factor (pdgf) and phorbol 12-myristate 13-acetate (pma) induced different changes, e.g., disruption of stress fibers and cell rounding. alpha-thrombin, ... | 1993 | 8276897 |
| [evidence of the existence of hemolysin ii from bacillus cereus: cloning the genetic determinant of hemolysin ii]. | the hemolysin genetic determinant distinct from cereolysin ab genetic determinant (lecithinase and sphingomyelinase genes) has been cloned in escherichia coli and bacillus subtilis cells as an ecori fragment (2.9 kb) of bacillus cereus vkm-b771 chromosome dna. the hemolytic product encoded by the cloned dna fragment possessed all the properties of hemolysin ii known to date: it was not inhibited by cholesterol, exhibited the arrhenius effect, and had a relatively long (in comparison with cereoly ... | 1993 | 8283976 |
| a soluble bacillus cereus cytochrome p-450cin system catalyzes 1,4-cineole hydroxylations. | a cytochrome p-450-dependent monooxygenase system that catalyzes the stereospecific hydroxylation of the monoterpene substrate 1,4-cineole was demonstrated in cell-free preparations of bacillus cereus ui-1477. 1,4-cineole hydroxylations were catalyzed by a 100,000 x g (1-h)-centrifuging soluble, hexane-inducible enzyme that activated and incorporated molecular oxygen into hydroxylated products; required nadh; was inhibited by skf-525a, imidazole, metyrapone, and octylamine; and displayed a 452-n ... | 1993 | 8285692 |
| antimicrobial activity of a newly identified bacteriocin of bacillus cereus. | a bacteriocin-producing bacillus cereus strain was isolated. the bacteriocin, here called cerein, was shown to be active specifically against other b. cereus strains and inactive against all other bacterial species tested. cerein was detected in the culture supernatants of stationary-phase cells, and its appearance was inhibited by induction of sporulation. the bacterial activity of cerein was insensitive to organic solvents and nonproteolytic enzymes, partially stable to heat, and active over a ... | 1993 | 8285719 |
| membrane-bound 5'-nucleotidase/nucleoside phosphotransferase from bacillus cereus. | 1. a search for nucleoside phosphotransferase activity in bacillus cereus led to the following results: (i) the phosphotransferase activity was associated with a membrane bound 5'-nucleotidase. (ii) the enzyme phosphorylates both purine and pyrimidine nucleosides as well as 2',3'-dideoxyinosine. (iii) the enzyme was inhibited by adenylic nucleotide di- and triphosphates, and its nucleotidase activity was increased in the presence of inosine as phosphate acceptor. 2. bacterial and vertebrate 5'-n ... | 1993 | 8288031 |
| [isolation of site-specific endonucleases and methylases from bacillus cereus 83]. | the site-specific endonuclease r bce83i and methylase m bce83i were isolated from bacillus cereus 83 by three consecutive chromatographies on blue agarose, hydroxyapatite and heparin-sepharose. r bce83i recognizes [formula: see text] sequences on the dna and cleaves the dna as indicated by the arrows. the endonuclease is stimulated by s-adenosyl-l-methionine and may consequently be referred to type iv restriction enzymes. | 1993 | 8292647 |
| toxin production by bacillus cereus in dairy products. | spores of a known toxigenic and psychrotrophic dairy isolate of bacillus cereus (hrm 44) were unable to grow and produce diarrhoeagenic toxin at 6 degrees c in creams and dairy-based products. these findings suggest that the production of b. cereus diarrhoeagenic toxin is unlikely to occur in creams and dairy-based products maintained within the cold chain. growth and toxin production were readily demonstrated in creams and some desserts stored at 21 degrees c. growth in creams was associated wi ... | 1993 | 8294609 |
| influence of ph and sugars on the growth and production of diarrhoeagenic toxin by bacillus cereus. | broth cultures supplemented with high levels of sugars, particularly glucose at > 50 milligrams, did not support diarrhoeagenic toxin production by psychrotrophic bacillus cereus despite growth to high counts (approximately 10(7)/ml) over a 4 d period of incubation at 21 degrees c. in contrast, starch levels of 10 and 50 milligrams actually enhanced toxin production. toxin production was also affected by ph levels of broth cultures, and was concomitant with alterations in bacterial growth. these ... | 1993 | 8294610 |
| [two cases of acute myelogenous leukemia with bacillus cereus bacteremia resulting in fatal intracranial hemorrhage]. | this manuscript reports bacillus cereus sepsis in two cases with acute myelogenous leukemia (aml) who suffered complications of fatal intracranial hemorrhage during remission induction therapy. the first case was 43-year-old male with aml (m0) receiving first consolidation chemotherapy who developed sudden diarrhea, abdominal pain and spiking fever. two days later, he died of intracranial hemorrhage. the second case was 15-year-old male with aml (m5b) who was receiving first induction chemothera ... | 1993 | 8295331 |
| formation of 1,3-cyclic glycerophosphate by the action of phospholipase c on phosphatidylglycerol. | the action of phospholipase c (plc) from bacillus cereus on phosphatidylglycerol (pg), derived from egg yolk phosphatidylcholine (pc), was examined in an ether-water mixture. the plc cleavage of pg and pc followed a michaelis-menten kinetics with apparent vmax values per 1 microgram enzyme of 0.26 and 0.91 mumol.min-1 and km values of 10 and 12 mm, respectively. when the same enzymic reaction was carried out in minimally buffered aqueous solution of 1% triton x-100, the decrease in ph with respe ... | 1993 | 8314778 |
| endogenous candida endophthalmitis. | 1993 | 8315640 | |
| biochemical and immunological properties of the c-terminal domain of the alpha-toxin of clostridium perfringens. | the c-terminal domain of the alpha-toxin (cpa247-370) of clostridium perfringens has been expressed in escherichia coli and purified. antiserum raised against cpa247-370 reacted in an identical manner to anti-alpha-toxin serum when used to map epitopes in the c-terminal domain, suggesting that cpa247-370 was immunologically and structurally identical to this region in the alpha-toxin. the isolated cpa247-370 was devoid of sphingomyelinase activity or haemolytic activity and was not cytotoxic for ... | 1993 | 8319893 |
| sphingomyelinase is part of the 'enterotoxin complex' produced by bacillus cereus. | the three components of the 'enterotoxin complex' have been purified and the sequence of the first 14-15 amino acids of the proteins determined. limited homology was found in the n-terminal sequence of the three proteins. the molecular mass of the proteins was determined to be 48, 40 and 34 kda, respectively. only the 40-kda protein was toxic to vero cells, whilst the 34-kda protein was found to be hemolytic. the sequence of the first 14 n-terminal amino acids of this protein was identical to th ... | 1993 | 8319899 |
| role of calcium in biphasic germination of bacillus cereus t spores sensitized to lysozyme. | biphasic germination induced by inosine in the presence of ca2+ was examined using bacillus cereus t spores treated with sodium dodecyl sulfate (sds) and dithiothreitol (dtt) at ph 10. the first phase of the germination was stimulated by ca2+ in the concentration-dependent manner, showing the optimal concentration at 0.5-1.0 mm. the second phase appeared to be insensitive to the cation. the optimal temperatures for the first and the second phase were 25 c and 40 c, respectively; the optimal phs ... | 1993 | 8321148 |
| nonspecific phospholipase c of listeria monocytogenes: activity on phospholipids in triton x-100-mixed micelles and in biological membranes. | listeria monocytogenes secretes a phospholipase c (plc) which has 39% amino acid sequence identity with the broad-specificity plc from bacillus cereus. recent work indicates that the l. monocytogenes enzyme plays a role during infections of mammalian cells (j.-a. vazquez-boland, c. kocks, s. dramsi, h. ohayon, c. geoffroy, j. mengaud, and p. cossart, infect. immun. 60:219-230, 1992). the homogeneous enzyme has a specific activity of 230 mumol/min/mg when phosphatidylcholine (pc) is dispersed in ... | 1993 | 8331063 |
| enterotoxigenic bacteria in the sudden infant death syndrome. | faecal samples from 123 infants who died with sudden infant death syndrome (sids) and from a comparative group of 52 age-matched babies were analysed for toxigenic bacteria and their toxins. serum samples from the sids infants were also analysed for these toxins. a significantly higher proportion of toxigenic bacteria and their toxins were found in faecal samples of sids babies than in samples from the comparative group. these toxins were also found in serum from the sids babies. clostridium per ... | 1993 | 8345506 |
| [bacillus cereus infection in orthopedic surgery]. | 1993 | 8347708 | |
| protein kinase c-dependent stimulation of phospholipase d in phospholipase c-treated fibroblasts. | treatment of [14c]choline- or [14c]ethanolamine-labeled nih 3t3 fibroblasts with bacillus cereus phosphatidyl-choline-specific phospholipase c (plc) enhanced phospholipase d (pld)-mediated hydrolysis of the respective 14c-labeled phospholipids. pld activity was stimulated by 1.5 u/ml of plc and by 100 nm of the protein kinase c (pkc) activator phorbol 12-myristate 13-acetate (pma) to similar extents. treatment of [14c]palmitic acid-labeled fibroblasts with plc in the presence of ethanol also enh ... | 1993 | 8355574 |
| purification and preliminary characterization of permethrinase from a pyrethroid-transforming strain of bacillus cereus. | bacillus cereus sm3 was isolated on a mineral salts medium with tween 80 as the primary carbon source. it was able to hydrolyze second- and third-generation pyrethroids, thereby generating noninsecticidal products. the enzyme responsible for this hydrolytic reaction was named permethrinase for this study. this is the first instance in which pyrethroid detoxification has been achieved with a cell-free microbial enzyme system. permethrinase was purified by ion-exchange chromatography and gel filtr ... | 1993 | 8357241 |
| distribution and identification of proteolytic bacillus spp. in paddy field soil under rice cultivation. | proteolytic bacteria in paddy field soils under rice cultivation were characterized and enumerated using azocoll agar plates. bacillus spp. were the proteolytic bacteria that were most frequently present, comprising 59% of the isolates. they were always the numerically dominant proteolytic bacteria isolated from three kinds of fertilizer treatments (yearly application of rice-straw compost and chemical fertilizer, yearly application of chemical fertilizer, and no fertilizer application) and at t ... | 1993 | 8364803 |
| phase separation of nonionic detergents by salt addition and its application to membrane proteins. | by adding salts (sodium chloride, ammonium sulfate), it is possible to induce phase separation in membrane-protein solubilisates containing triton x-100 or nonidet p-40 at temperatures between 0 and 20 degrees c. other nonionic detergents of the brij, lubrol, and tween series can also be used for this procedure. the salt concentration required for induction of phase separation is dependent on the hydrophobicity of the detergent used. for detergents of the triton series it seems that detergents w ... | 1993 | 8368489 |
| polypeptide folding of bacillus cereus atcc7064 oligo-1,6-glucosidase revealed by 3.0 a resolution x-ray analysis. | the crystal structure of an oligo-1,6-glucosidase from bacillus cereus atcc7064 was determined by the x-ray diffraction method at 3.0 a resolution. the structure was solved by the multiple isomorphous replacement method and refined to a crystallographic r-factor of 0.208, using the molecular dynamics refinement program, x-plor. the electron density map revealed the folding of a polypeptide chain consisting of 558 amino acid residues. the molecule can be subdivided into three domains (n-terminal ... | 1993 | 8370659 |
| the oxazolidinone antibacterial agent dup 105 does not act on cell wall biosynthesis or on a beta-lactamase. | (s)-3-(4-methylsulfinylphenyl)-5-(acetamidomethyl)oxazolidinone (dup 105; 1, r = ch3so, r' = ac), one of a new class of antibacterial agents, is shown to be devoid of inactivation and inhibitory properties toward bacterial cell wall biosynthesis and toward a beta-lactamase. this is consistent with the idea that this class of compounds acts by a route different from that of the beta-lactam antibiotics. | 1993 | 8373386 |
| lipoprotein lipase and sphingomyelinase synergistically enhance the association of atherogenic lipoproteins with smooth muscle cells and extracellular matrix. a possible mechanism for low density lipoprotein and lipoprotein(a) retention and macrophage foam cell formation. | prominent features of atheromata include smooth muscle cells, cholesteryl ester-loaded macrophage foam cells, extracellular matrix, extracellularly trapped and aggregated lipoproteins, and various enzymes including lipoprotein lipase (lpl) and sphingomyelinase (smase). the interplay of these factors was investigated in cell culture. incubation of bovine aortic smooth muscle cells for 18 h at 37 degrees c with low density lipoprotein (ldl) in the presence of lpl and smase led to massive aggregati ... | 1993 | 8376399 |
| dissemination of bacillus cereus in an intensive care unit. | to report the contamination of ventilator equipment with bacillus cereus and to outline the measures taken to trace the source of the organism. | 1993 | 8376735 |
| dicranin, an antimicrobial and 15-lipoxygenase inhibitor from the moss dicranum scoparium. | extracts of nine mosses, collected in switzerland, were screened for antimicrobial, antioxidative, and 15-lipoxygenase (15-lpo) inhibitory activities. the ch2cl2 extract of dicranum scoparium was found to possess pronounced antimicrobial activity against bacillus cereus, bacillus stearothermophilus, bacillus subtilis, staphylococcus aureus, and escherichia coli. in addition, inhibition of soybean 15-lpo occurred at very low concentration. phytochemical investigation of this extract afforded z,z, ... | 1993 | 8377015 |
| type selective inhibition of microbial fatty acid synthases by thiolactomycin. | the antibiotic, thiolactomycin, is known to selectively inhibit the type ii straight-chain fatty acid synthase (monofunctional enzyme system, e.g. escherichia coli enzyme) but not type i straight-chain fatty acid synthase (multifunctional enzyme system, e.g. saccharomyces cerevisiae enzyme). we have studied the effect of thiolactomycin on the branched-chain fatty acid synthases from bacillus subtilis, bacillus cereus, and bacillus insolitus. fatty acid synthase from all three bacilli was not inh ... | 1993 | 8379811 |
| antimicrobial activity and spectrum of rifaximin, a new topical rifamycin derivative. | rifaximin, a rifamycin derivative, was evaluated in vitro to assess its spectrum and potency against a wide variety of bacteria, yeasts, viruses, and parasites. high concentrations of rifaximin were often used to reflect topically achieved levels since this compound is poorly absorbed by oral route. like rifampin, rifaximin possessed best activity against staphylococcus spp. (mic50 < or = 0.015 microgram/ml), streptococcus spp. (mic50s, < or = 0.03-0.12 microgram/ml), enterococcus spp. (mic50s, ... | 1993 | 8385592 |
| crystallization of phosphatidylinositol-specific phospholipase c from bacillus cereus. | phosphatidylinositol-specific phospholipase c (pi-plc) cleaves phosphoinositides into two parts, lipid-soluble diacylglycerol and the water-soluble phosphorylated inositol. two crystal forms of bacillus cereus pi-plc have been obtained by the vapor diffusion technique. hexagonal crystals were grown from solutions containing polyethylene glycol (peg; 4,000 to 8,000 d). the space group of these hexagonal crystals is p6(1)22 (or the enantiomorphic space group p6(5)22), with cell constants a = b = 1 ... | 1993 | 8386017 |
| [nucleotide sequence of phospholipase c and sphingomyelinase genes from bacillus cereus bkm-b164]. | a bamhi-ecorv dna fragment, containing tandemly arranged genes for the bacillus cereus vkm-b164 phosphatidylcholine-specific phospholipase c and sphingomyelinase, has been cloned and sequenced in puc19 plasmid (embl accession no x64141). the obtained gene sequences have been compared with those the b. cereus strains se-1, iam-1208 and gp-4 reported previously. both phospholipase c and sphingomyelinase gene sequences of the vkm-b164 strain were found to be identical to those of the strain se-1 an ... | 1993 | 8387306 |
| sequence of the salmonella typhimurium stylt1 restriction-modification genes: homologies with ecop1 and ecop15 type-iii r-m systems and presence of helicase domains. | the stylt1 restriction-modification (r-m) system of salmonella typhimurium has recently been suggested to belong to the type-iii r-m systems [de backer and colson, gene 97 (1991) 103-107]. the nucleotide sequences of stylt1 mod and res have been determined. two closely adjacent open reading frames were found 12 bp apart with coding capacities of 651 (mod) and 982 (res) amino acids (aa), respectively. the genes, lying in the same direction of transcription in the mod-res order, are transcribed as ... | 1993 | 8387444 |
| [study of the nature of determinants for synthesis of delta-endotoxin and beta-exotoxin in bacillus thuringiensis subsp. thuringiensis strains--bitoxybacillin producers]. | the growth of bacillus thuringiensis subsp. thuringiensis strains (bt) 202, 1140, 98 producing bitoxybacillin in the presence of novobiocin, mitomycin c and at high temperature 43 degrees c resulted in obtaining of the mutants for the synthesis of crystalline protein (cry) and exotoxin (exo). analysis of the plasmid content of the mutants has shown the cry exo phenotyre to correlate with the loss of 55 md plasmid in the strain bt202 and the loss of 60md plasmid in bt1140. the transfer of these p ... | 1993 | 8389984 |
| developmental and biochemical characteristics of the cardiac membrane-bound arginine-specific mono-adp-ribosyltransferase. | adp-ribosylation of protein in heart membrane preparations has been shown to be present in adult tissue but absent from early neonate tissue [piron and mcmahon (1990) biochem. j. 270, 591-597]. to further this observation, the cardiac membrane-bound form of arginine-specific mono-adp-ribosyltransferase (ec 2.4.2.31) has been characterized. apparent km values of 330 and 470 microm were found in heart membrane preparations from rat and quail respectively. the vmax. value depended greatly on the sp ... | 1993 | 8394692 |
| human erythrocyte sialidase is linked to the plasma membrane by a glycosylphosphatidylinositol anchor and partly located on the outer surface. | treatment of human erythrocyte ghosts with phosphatidylinositol-phospholipase c (piplc) from bacillus cereus liberated the ghost-linked sialidase. maximal release of sialidase (about 70% of total) was achieved by incubating ghosts at 37 degrees c for 60 min, at ph 6.0, with piplc (piplc total units/ghost protein ratio, 4.5 each time) added at the beginning of incubation and every 15 min (four subsequent additions). liberated sialidase was fully resistant to at least four cycles of rapid freezing ... | 1993 | 8395269 |
| citrinin-producing capacity of penicillium expansum strains from apple packinghouses of lerida (spain). | a study of the citrinin producing capacity in 122 penicillium expansum strains isolated from apples and various locations of apple packaging premises lleida (spain) was conducted. among the strains examined, 46% produced citrinin in a laboratory substrate and 73.2% of these strains were isolated from decayed apples. all citrinin producing strains produced metabolites which inhibited bacillus cereus and bacillus subtilis. the majority of the strains producing citrinin in solid substrate also prod ... | 1993 | 8398629 |
| degradation of plasma membrane phosphatidylcholine appears not to affect the cellular cholesterol distribution. | to clarify the role of possible cholesterol/phosphatidylcholine interactions in cellular cholesterol distribution, we have used a phosphatidylcholine-specific phospholipase c from bacillus cereus to degrade the cell surface phosphatidylcholine of cultured human fibroblasts. of cellular phosphatidylcholine, approximately 15% was susceptible to degradation by the phospholipase. in spite of the dramatic redistribution of cellular cholesterol that can be observed after sphingomyelin depletion, the d ... | 1993 | 8409769 |
| phosphatidylcholine hydrolysis activates nf-kappa b and increases human immunodeficiency virus replication in human monocytes and t lymphocytes. | we have tested whether breakdown of phosphatidylcholine (pc) initiated by exogenous addition of a pc-specific phospholipase c (pc-plc) from bacillus cereus or by endogenous overexpression of pc-plc induces functional activation of nf-kappa b and increases human immunodeficiency virus (hiv) enhancer activity. pc-plc-activated hydrolysis of pc was found to induce bona fide p50/p65 nf-kappa b binding activity in three different cell lines of human or murine origin. no significant changes in the tur ... | 1993 | 8411362 |
| detection by in vitro amplification of the alpha-toxin (phospholipase c) gene from clostridium perfringens. | a polymerase chain reaction (pcr) with thermostable dna polymerase from thermus aquaticus is described for the specific amplification of the phospholipase c (alpha-toxin) gene of clostridium perfringens. a set of primers selected for their high specificity could detect cl. perfringens in stools with a detection limit of approximately 5 x 10(2) bacteria, after bi-amplification. a modified pcr without thermal steps was performed to rapidly amplify, with a yield of 60%, the dna template. with this ... | 1993 | 8420919 |
| phospholipase c-promoted membrane fusion. retroinhibition by the end-product diacylglycerol. | the catalytic activity of phospholipase c induces fusion of pure lipid vesicles. when large unilamellar liposomes composed of phosphatidylcholine/phosphatidylethanolamine/cholesterol (2:1:1 mole ratio) are treated with phospholipase c, in the presence of 10 mm ca2+, two enzyme effects can be distinguished: a fast one (half-time on the order of seconds) consisting mainly of vesicle-vesicle fusion and a slow one (half-time on the order of minutes) representing bulk lipid hydrolysis. the fast fusio ... | 1993 | 8424934 |
| nmr studies of phospholipase c hydrolysis of phosphatidylcholine in model membranes. | hydrolysis of phospholipids in biological membranes by phospholipase c (plc) produces an important second messenger molecule, 1,2-diacylglycerol (dag), that is essential for the activation of protein kinase c (pkc). while the effects of dag on model membranes have been investigated earlier, studies on physical properties of dag introduced into phospholipid bilayers by plc have been lacking. we present an nmr approach for studying structural and kinetic aspects of plc-mediated hydrolysis of 13 ca ... | 1993 | 8428917 |
| a complete physical map of a bacillus thuringiensis chromosome. | bacillus thuringiensis is the source of the most widely used biological pesticide, through its production of insecticidal toxins. the toxin genes are often localized on plasmids. we have constructed a physical map of a bacillus thuringiensis chromosome by aligning 16 fragments obtained by digestion with the restriction enzyme noti. the fragments ranged from 15 to 1,350 kb. the size of the chromosome was 5.4 mb. the noti dna fingerprint patterns of 12 different b. thuringiensis strains showed mar ... | 1993 | 8432698 |
| cloning of the beta-amylase gene from bacillus cereus and characteristics of the primary structure of the enzyme. | the gene encoding the beta-amylase of bacillus cereus bq10-s1 (spoii) was cloned into escherichia coli jm 109. a sequenced dna fragment of 2,001 bp contains the beta-amylase gene. the n-terminal sequences (avngkg mnpdykaylmaplkki), the c-terminal sequences (shtssw), and the amino acid sequences of the five regions in the beta-amylase molecules were determined. the mature beta-amylase contains 514 amino acid residues with a molecular mass of 57,885 da. the amino acid sequence homology with those ... | 1993 | 8434930 |
| the antimicrobial effect of a structural variant of subtilin against outgrowing bacillus cereus t spores and vegetative cells occurs by different mechanisms. | subtilin is a ribosomally synthesized antimicrobial peptide that contains several unusual amino acids as a result of posttranslational modifications. site-directed mutagenesis was employed to construct a structural variant of subtilin in which the unusual dehydroalanine (dha) residue at position 5 was changed to alanine. proton nuclear magnetic resonance spectroscopy, amino acid composition, and n-terminal sequence analysis established that the mutation did not disrupt posttranslational processi ... | 1993 | 8434932 |
| epigrisorixin, a new polyether carboxylic antibiotic. | 1993 | 8436555 | |
| arbitrary primer polymerase chain reaction, a powerful method to identify bacillus thuringiensis serovars and strains. | arbitrary primer polymerase chain reaction technology has been applied to the identification of commercial strains of bacillus thuringiensis by using total dnas extracted from single bacterial colonies as templates. characteristic dna banding patterns can be readily and reproducibly obtained by agarose gel electrophoresis. this method has been used to distinguish commercial products containing b. thuringiensis serovar kurstaki (3a3b). when a single primer was used this method was capable of prod ... | 1993 | 8439143 |
| phosphatidylcholine hydrolysis and c-myc expression are in collaborating mitogenic pathways activated by colony-stimulating factor 1. | stimulation of diglyceride production via phospholipase c (plc) hydrolysis of phosphatidylcholine was an early event in the mitogenic action of colony-stimulating factor 1 (csf-1) in the murine macrophage cell line bac1.2f5 and was followed by a second phase of diglyceride production that persisted throughout the g1 phase of the cell cycle. addition of phosphatidylcholine-specific plc (pc-plc) from bacillus cereus to the medium of quiescent cells raised the intracellular diglyceride concentratio ... | 1993 | 8441394 |
| freeze-dried mixed cultures as reference samples in quantitative and qualitative microbiological examinations of food. | two simulated food samples in the form of mixtures of bacteria, freeze-dried in small glass vials, were tested for their suitability as reference materials in food microbiology. stability and homogeneity of the samples were assessed during a 1 year period when stored at 2-6 degrees c. samples were examined for aerobic plate counts, coliform counts, staphylococcus aureus, clostridium perfringens, bacillus cereus, enterococci, yeasts and salmonellas. the samples were homogeneous but the number of ... | 1993 | 8444643 |
| oral ciprofloxacin therapy for bacillus cereus wound infection and bacteremia. | 1993 | 8448312 | |
| bacillus cereus pneumonia in premature neonates: a report of two cases. | 1993 | 8451107 | |
| determination of carbohydrate profiles of bacillus anthracis and bacillus cereus including identification of o-methyl methylpentoses by using gas chromatography-mass spectrometry. | bacillus anthracis and bacillus cereus are closely related pathogenic organisms that are difficult to differentiate phenotypically or genotypically. it is well known that vegetative and spore forms of bacilli are quite distinct both morphologically and chemically, but spore-specific chemical markers allowing these species to be distinguished have not been previously described. by using gas chromatography-mass spectrometry, vegetative cells and spores of the two species were shown to exhibit dist ... | 1993 | 8463400 |
| rapid screening test for enterotoxin-producing bacillus cereus. | culture supernatants of 30 enterotoxin-producing bacillus cereus isolates produced a characteristic progressive destruction of mccoy cell monolayers. enterotoxin-negative b. cereus and other group 1 bacillus spp. caused no monolayer disruption. the mccoy cell tissue culture system appears to provide a rapid screening assay for detection of enterotoxin-producing b. cereus. | 1993 | 8463405 |
| prokaryotic members of a new family of putative helicases with similarity to transcription activator snf2. | cloning and sequence analysis of a new open reading frame from bacillus cereus reveals the relationship to a recently identified family of putative eukaryotic transcription activators similar to the yeast snf2 gene product. as a result of comparative analysis of sequence features conserved in all members of this family, a gene from a chilo iridescent virus, as well as a putative helicase from escherichia coli (hepa), can also be grouped into this family. the unexpected presence of prokaryotic an ... | 1993 | 8464078 |
| analysis of enterotoxin production by bacillus cereus from dairy products, food poisoning incidents and non-gastrointestinal infections. | of 85 strains of bacillus cereus isolated in norway from dairy products, 59% were found to be enterotoxigenic, and 15% were psychrotrophic. six of the isolates (7%) were identified as potential psychrotrophic food-poisoning strains as they were both enterotoxigenic and exhibited good growth at 6 degrees c. enterotoxin production was detected using the western immunoblot technique, and a commercially available reversed passive latex agglutination (rpla) assay (unipath bcet-rpla td950). both metho ... | 1993 | 8466800 |
| effect of three preservatives on the growth of bacillus cereus, vero cytotoxigenic escherichia coli and staphylococcus aureus, on plates with gradients of ph and sodium chloride concentration. | the effect of temperature, ph, sodium chloride concentration and a preservative (sodium benzoate, sodium nitrite or potassium sorbate) on the growth of three foodborne bacterial pathogens (bacillus cereus, vero cytotoxigenic escherichia coli and staphylococcus aureus) was studied using gradient gel plates. growth, expressed in optical density units, was recorded using image analysis techniques, and was expressed as three-dimensional grids. these gave a visual indication of the effects of any thr ... | 1993 | 8466802 |
| effect of low-dose irradiation on growth of and toxin production by staphylococcus aureus and bacillus cereus in roast beef and gravy. | the effect of irradiation (2 kgy) on growth of and toxin production by staphylococcus aureus and bacillus cereus in roast beef and gravy during storage at abuse temperatures (15 and 22 degrees c) was assessed by inoculation studies. irradiation resulted in a 3-4 log10 reduction in numbers of both pathogens. whenever b. cereus and s. aureus numbers reached 10(6) and 10(7) cfu/g, respectively, during storage their toxins were detectable. as the time taken to attain these levels was longer in irrad ... | 1993 | 8466810 |
| microbial flora of frozen tail meat from captive nile crocodiles (crocodylus niloticus). | frozen tail meat samples from nine nile crocodiles (crocodylus niloticus) were evaluated for microbial numbers and types. microbial analyses yielded a mean aerobic plate count (apc, 30 degrees c) of 4.86 log cfu/g, a mean coliform count (tc, 37 degrees c) of 3.77 log cfu/g and a mean faecal coliform count (fc, 44 degrees c) of 3.40 log cfu/g. salmonella was isolated from three samples, and beta-haemolytic streptococci from two samples. yersinia, aeromonas, staphylococcus aureus and bacillus cere ... | 1993 | 8466815 |
| an overview of the kinetic parameters of class b beta-lactamases. | the catalytic properties of three class b beta-lactamases (from pseudomonas maltophilia, aeromonas hydrophila and bacillus cereus) were studied and compared with those of the bacteroides fragilis enzyme. the a. hydrophila beta-lactamase exhibited a unique specificity profile and could be considered as a rather specific 'carbapenemase'. no relationships were found between sequence similarities and catalytic properties. the problem of the repartition of class b beta-lactamases into sub-classes is ... | 1993 | 8471035 |
| analysis of common antigen of flagella in bacillus cereus and bacillus thuringiensis. | flagellar antigen of bacillus cereus h.1 was purified and tested for serodiagnostic antigen by elisa. the antibody against the flagellar antigen of b. cereus h.1 reacted not only with the homologous specific antigen but also reacted with the flagellar antigens of 23 strains of b. cereus. this common flagellar antigen of b. cereus was found to be due to 61-kda protein by sds-page and immunoblot assay. monoclonal antibody h15a5 against common antigenic epitope of b. cereus also reacted with flagel ... | 1993 | 8472901 |
| inhibitory effect of 8-(n,n-diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (tmb-8) on germination of bacillus cereus t spores. | effect of 8-(n,n-diethylamino)octyl-3,4,5-trimethoxybenzoate hydrochloride (tmb-8), a calcium antagonist, on germination of bacillus cereus t spores induced by l-alanine and inosine was investigated. tmb-8 had no effect on the germination of heat-activated spores, whereas it inhibited that of nonactivated spores. the tmb-8 inhibitory effect was antagonized competitively by inosine, but not by l-alanine. addition of ca2+ reversed the inhibitory effect of tmb-8 in a dose-related fashion. based on ... | 1993 | 8474359 |
| effect of various digestive enzymes on the interaction of toxoplasma gondii with macrophages. | the participation of resident, elicited, and activated macrophage surface components during internalization of tachyzoites of toxoplasma gondii was analyzed using neuraminidase, phospholipase c, trypsin, protease, and hyaluronidase. treatment of these macrophages with neuraminidase from vibrio cholerae, phospholipase c from bacillus cereus and clostridium perfringens, protease, and hyaluronidase prior to their interaction with parasites increased the penetration of host cells by t. gondii. incub ... | 1993 | 8475028 |
| genetic construction, expression, and characterization of a single chain anti-carcinoma antibody fused to beta-lactamase. | we report the genetic construction and expression of a fusion protein between an antibody single chain-linked variable domain fragment specific for human carcinomas and beta-lactamase ii from bacillus cereus. sequences encoding the variable regions of the l6 monoclonal antibody were assembled so as to be separated from each other by an 18-amino acid linker and from the mature form of beta-lactamase by a 6-amino acid linker. the construct was placed under the transcriptional regulation of the lac ... | 1993 | 8481914 |
| [characteristics of phospholipid hydrolysis kinetics by phospholipase c from bacillus cereus. hydrolysis of phosphatidylinositol in various aggregated states]. | the process of phospholipase c hydrolysis of phosphatidylethanolamine (pe) in the form of mixed micelles phospholipid-detergent or in the form of vesicules in the mixture with phosphatidylcholine (pc) was studied. the size of the micelles was measured by dynamic light-scattering and their structure was determined by 31p-nmr spectroscopy. it was found that the kinetics of pe hydrolysis in the micelles by phospholipase c from bacillus cereus do not follow michaelis-menten equation, but at all conc ... | 1993 | 8485222 |
| differential translocation of protein kinase c isozymes by thrombin and platelet-derived growth factor. a possible function for phosphatidylcholine-derived diacylglycerol. | the translocation of protein kinase c (pkc) from the cytosolic to the particulate fraction in iic9 fibroblasts has been studied to define the functions of 1,2-diacylglycerol (dag) derived from the hydrolysis of phosphatidylinositol 4,5-bisphosphate (pip2) and phosphatidylcholine (pc). alpha-thrombin caused a biphasic change in dag, with two peaks at 15-60 s and 5-15 min, derived from pip2 and pc, respectively, while platelet-derived growth factor (pdgf) induced a monophasic dag increase from pc ... | 1993 | 8486706 |
| the penetration of oral ciprofloxacin into the aqueous humor, vitreous, and subretinal fluid of humans. | we examined ciprofloxacin levels in the aqueous humor, vitreous, or subretinal fluid in 40 patients undergoing cataract extraction, vitrectomy, or scleral buckling. ciprofloxacin, 750 mg, was administered orally an average of 17 1/2 and 5 1/2 hours preoperatively. we obtained mean ciprofloxacin levels of 0.53 microgram/ml in aqueous humor, 0.51 microgram/ml in vitreous, and 0.71 microgram/ml in subretinal fluid. these vitreous levels exceed the minimum inhibitory concentration (mic)90 of staphyl ... | 1993 | 8488915 |
| a large outbreak of gastroenteritis caused by diarrheal toxin-producing bacillus cereus. | an outbreak of diarrhea occurred after a university field day. of 643 attendees who returned mailed questionnaires, 139 (22%) reported illness. persons who ate barbecued pork, which was unrefrigerated for 18 h after cooking, were five times more likely to become ill than those who did not eat pork (26% vs. 5%; relative risk, 5.4; 95% confidence interval, 1.4-20.9). a leftover pork sample grew a bacillus cereus isolate, > 10(5) cfu/g, that produced diarrheal toxin. thirty-four percent of ill pers ... | 1993 | 8501338 |
| lipophosphoglycan antigen shedding by leishmania donovani. | the biochemical characterizations of lipophosphoglycans from various leishmania species reported by other workers may or may not contain several types of lipophosphoglycan molecules. this is the first report in which a specific lipophosphoglycan has been defined by both its antigenic and electrophoretic properties. furthermore, a purification procedure for this specific lipophosphoglycan is described and some biochemical characterizations are presented. phospholipase c and the so-called phosphat ... | 1993 | 8508171 |
| properties of the cold-labile nad(+)-specific glutamate dehydrogenase from bacillus cereus dsm 31. | nicotinamide-adenine-dinucleotide-specific glutamate dehydrogenase (nad-gdh; ec 1.4.1.3) from bacillus cereus dsm 31 was enriched 260-fold. the molecular mass was determined by gel filtration to be 270 kda (+/- 25 kda). the enzyme was highly specific for the coenzyme nad(h) and catalysed both the formation and the oxidation of glutamate. apparent km values of 7.7 mm for glutamate and 0.56 mm for nad+ during oxidative deamination were measured. both in crude cell-free extracts and in enriched pre ... | 1993 | 8515235 |
| the crystal structure of tris-inhibited phospholipase c from bacillus cereus at 1.9 a resolution. the nature of the metal ion in site 2. | we report here the crystal structure of the complex formed between phospholipase c (plc) from bacillus cereus and the widely used biochemical buffer tris (hydroxymethyl)-methylamine (tris). the structure has been determined at 1.9 a resolution and refined to r = 20.3%. tris has metal-binding properties, especially to zn2+, and has been reported to reduce the activity of plc. the amine nitrogen atom in tris is co-ordinated to one of the three zn2+ ions in the active site of the enzyme, thus confi ... | 1993 | 8515456 |
| biochemical properties of a carbapenem-hydrolyzing beta-lactamase from enterobacter cloacae and cloning of the gene into escherichia coli. | a clinical isolate of enterobacter cloacae, strain nor-1, exhibited resistance to imipenem and remained susceptible to extended-spectrum cephalosporins. clavulanic acid partially restored the susceptibility of the strain to imipenem. two beta-lactamases with isoelectric points (pi) of 6.9 and > 9.2 were detected in strain e. cloacae nor-1; the higher pi corresponded to ampc cephalosporinase. plasmid dna was not detected in e. cloacae nor-1 and imipenem resistance could not be transferred into es ... | 1993 | 8517720 |
| unusual chromatographic behaviour and one-step purification of a novel membrane proteinase from bacillus cereus. | cell envelopes of bacillus cereus contain a casein-cleaving membrane proteinase (ccmp) and an insulin-cleaving membrane proteinase (icmp), which differ in their substrate and inhibitor specificity from all bacillus proteinases described previously. they remained localized in the cytoplasmic membrane after treatment with lysozyme and mutanolysin and they are strongly attached to the membrane compared with other known membrane proteinases. only high a concentration of the zwitterionic detergent su ... | 1995 | 8520670 |
| microbial bioassays to assess the toxicity of solid-associated contaminants. | due to the effects that sediment or soil matrices have on the bioavailability of compounds, it has been difficult to screen toxicity of solid-associated contaminants. the majority of microbial assays for testing toxicity of soils and sediments have been performed on water or solvent extracts. these procedures lead to a fractionation of the toxicity, which may underestimate or overestimate exposure routes and consequently potential adverse environmental effects. recently, a solid-phase microtox a ... | 1995 | 8521786 |
| [examination of toxin production from environmental bacillus cereus and bacillus thuringiensis]. | emetic toxin- and entero toxin-producing activities were examined for 524 strains of bacillus cereus and 90 strains of bacillus thuringiensis to determine the distribution and contamination in natural environment, including foods, vegetable, soil and etc. emetic toxin was assayed by the production of acid from hep-2 cells induced by the culture supernatants of bacillus, which was developed as an improved method for the detection of b. cereus heat-stable emetic-toxin by us. in the 524 strains of ... | 1995 | 8523268 |
| bacillus thuringiensis subsp. oswaldocruzi and bacillus thuringiensis subsp. brasiliensis, two novel brazilian strains which determine new serotype h38 and h39, respectively. | 1995 | 8524081 | |
| an unusual outbreak of food poisoning. | on august 25 1990, over 400 people who attended a thailand handicappeds' sport day at a provincial physical education college developed gastrointestinal symptoms after having dinner. an epidemiological team want to determine causes(s) and recommend how to prevent and control a food poisoning outbreak. the investigation included interviewing all 1,210 persons who attended the sport's day. in addition, an environmental survey, laboratory analysis of food samples, and rectal, ear, throat and nasal ... | 1995 | 8525424 |
| identification of a region of genetic variability among bacillus anthracis strains and related species. | the identification of a region of sequence variability among individual isolates of bacillus anthracis as well as the two closely related species, bacillus cereus and bacillus mycoides, has made a sequence-based approach for the rapid differentiation among members of this group possible. we have identified this region of sequence divergence by comparison of arbitrarily primed (ap)-pcr "fingerprints" generated by an m13 bacteriophage-derived primer and sequencing the respective forms of the only ... | 1996 | 8550456 |
| be-24566b, a new antibiotic produced by streptomyces violaceusniger. | 1995 | 8557611 | |
| ionophore properties of monensin derivatives studied on human erythrocytes by 23na nmr and k+ and h+ potentiometry: relationship with antimicrobial and antimalarial activities. | eight derivatives of monensin with a modified c25-c26 moiety were synthesized. their ionophore properties were studied on human erythrocytes by measuring na+ influx with 23na nmr and concomitant k+ and h+ efflux by potentiometry. modification of oh-26 led to inversion of selectivity of transport in favor of k+/na+ in comparison with monensin. this selectivity disappeared by suppression of the c26-oh moiety. finally the ionophore ability was lost if the head-to-tail chelation of the monensin skel ... | 1996 | 8558531 |
| some pharmacokinetic parameters of doxycycline in east african goats after intramuscular administration of a long-acting formulation. | a compartmental and non-compartmental study was carried out on five adult goats following intramuscular administration of doxycycline at 20 mg/kg bodyweight. the concentration of the drug in serum was determined by a microbiological assay employing bacillus cereus var mycoides (atcc 11778) as the test organism. the mean serum concentration (cmax) and the time of maximum concentration (tmax) were 1.87 micrograms/ml and 0.85 h, respectively. using compartmental analysis, the plasma concentration-t ... | 1995 | 8560757 |
| analysis of double-stranded polymerase chain reaction products from the bacillus cereus group by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry. | the analysis of polymerase chain reaction (pcr) products by electrospray ionization-fourier transform ion cyclotron resonance mass spectrometry (esi-fticr) has been achieved. specifically, a 105 base-pair nucleotide portion of the ribosomal spacer region was amplified in two members of the b. cereus group (i.e. b. thuringiensis and b. cereus) using pcr. these amplified regions were then analyzed by gel electrophoresis and esi-fticr. based on the predicted sequence of the pcr products for each or ... | 1996 | 8563015 |
| studies on microbial aerobic flora of skin in leprosy patients. | this study reports the isolation and identification of aerobic organisms from biopsies/slit-skin smears/scrapings from 129 leprosy patients and 50 healthy controls. these include 56 paucibacillary (pb) and 73 multibacillary (mb) cases. thirty-six isolates from the specimens from 21 patients and 15 healthy controls were grown. the non-mycobacterial isolates from clinically pb leprosy (tt/bt/i) patients were: (1) gram-positive cocci: staphylococcus aureus(1), staphylococcus albus(1); (b) gram-posi ... | 1995 | 8576610 |
| isolation, and virulence profiles, of aeromonas hydrophila implicated in an outbreak of food poisoning in sweden. | a case of food poisoning outbreak involving aeromonas hydrophila is reported in this study. a group of 27 people consumed a typical swedish food "landgång" which is a type of smörgåsbord containing shrimps with mayonnaise, liver paté, ham, sausage, and legume salad which was purchased from a food store. twenty-two of the 27 persons became ill within 20-34 hr of consumption of the food and reported the symptoms ranging from severe acute diarrhea, abdominal pain, headache, fever and vomiting. one ... | 1995 | 8577278 |
| characteristics of some psychrotrophic bacillus cereus isolates. | twelve strains of bacillus cereus isolated from different food products and foodborne disease outbreaks, and able to grow at temperatures < 7 degrees c, were characterised. generation times at 7 degrees c varied from 9.4 h up to 75 h. lag phase of the vegetative cells at 7 degrees c was strongly influenced by the previous temperature history of the cells. preincubation at 37 degrees c increased the duration of the lag phase drastically. the heat resistance at 90 degrees c (d90 degrees c-values i ... | 1995 | 8579988 |
| useful visual outcomes after treatment of bacillus cereus endophthalmitis. | bacillus cereus endophthalmitis occurring after penetrating ocular trauma has been almost always associated with a poor visual outcome. the purpose of our study was to review and report patients who had useful visual acuity outcomes. | 1996 | 8600414 |
| determination of lipid ester ozonides and core aldehydes by high-performance liquid chromatography with on-line mass spectrometry. | unsaturated triacylglycerols (tg) and choline (pc) and ethanolamine (pe) phosphatides of known structure were subjected to ozonization and reduction with triphenylphosphine to yield the corresponding lipid ester core aldehydes. mono- and di-c9 aldehyde palmitoylglycerols were prepared from oleoyldipalmitoyl and oleoyllinoleoylpalmitoyl glycerols, respectively, while egg yolk pc and pe provided the mono-c5 and mono-c9 aldehydes of palmitoyl-and stearoyl glycerophospholipids. the aldehydes were is ... | 1995 | 8621886 |
| successful visual recovery in delayed onset bacillus cereus endophthalmitis. | 1996 | 8630757 | |
| identification and purification of a family of dimeric major cold shock protein homologs from the psychrotrophic bacillus cereus wsbc 10201. | whole-cell protein patterns of a psychrotrophic bacillus cereus strain from cultures grown at 7 and 30 degrees c were compared. this analysis revealed that at least three major proteins are expressed at a significantly higher rate at 7 degrees c than at 30 degrees c. the most abundant of these cold-induced proteins was a small polypeptide of 7.5 kda, designated cspa, of b. cereus. in addition, four small proteins very similar in size to cspa were seen on both 7 degrees c and 30 degrees c two-dim ... | 1996 | 8631682 |
| tincture of benzoin: clinical and microbiological implications of reusable containers. | at our institution, tincture of benzoin solution is commonly used as a topical adhesive agent. as a cost-saving practice, multiple-dose bottles are routinely used in the operating rooms and the clinic on multiple patients. although clinically pathogenic organisms are known to be capable of survival in both benzoin and its isopropyl alcohol solvent, no prior controlled studies have investigated the potential for tincture of benzoin solution to support the growth of specific pathogens under clinic ... | 1996 | 8637641 |
| beta-secondary and solvent deuterium kinetic isotope effects on beta-lactamase catalysis. | beta-secondary and solvent deuterium kinetic isotope effects have been determined for the steady-state kinetic parameters v/k and v for turnover of a depsipeptide substrate, m-[[(phenylacetyl)glycyl]-oxy]benzoic acid, and of a beta-lactam substrate, penicillanic acid, by three typical class a beta-lactamases and a class c beta-lactamase. the isotope effects on alkaline hydrolysis of these substrates have been used as a frame of reference. the effect of the transition state conformation of the su ... | 1996 | 8639512 |
| syntheses and antimicrobial activities of five-membered ring heterocycles coupled to indole moieties. | indole-substituted oxazolidinones, oxazolones, pyrrolidinone, imidazolidinone and imidazolones were synthesized. their inhibitory potencies towards protein kinase c and protein kinase a were determined and their in vitro activities against streptomyces chartreusis, streptomyces griseus, bacillus cereus, candida albicans and escherichia coli were examined. the inhibition of streptomyces sporulation observed for some of them could not be linked to in vitro protein kinase c inhibition. all proved i ... | 1996 | 8642002 |
| dual inhibitory effect of gangliosides on phospholipase c-promoted fusion of lipidic vesicles. | the effect of a variety of gangliosides has been tested on the phospholipase c-induced fusion of large unilamellar vesicles. bilayer composition was phosphatidylcholine:phosphatidylethanolamine: cholesterol (2:1:1 mole ratio) plus the appropriate amounts of glycosphingolipids. enzyme phosphohydrolase activity, vesicle aggregation, mixing of bilayer lipids and mixing of liposomal aqueous contents were separately assayed. small amounts ( < 1 mol %) of gangliosides in the lipid bilayer produce a si ... | 1996 | 8652529 |
| sepsis associated with blood transfusion. | 1996 | 8656939 |