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bacterial microbiota of the ocular surface of captive and free-ranging microbats: desmodus rotundus, diameus youngi and artibeus lituratus.to investigate normal aerobic bacterial microbiota of the eye surface in vvcaptive and free-ranging bats belonging to the suborder microchiroptera.201423710696
evaluation of the in vitro activity of fosfomycin tromethamine against gram-negative bacterial strains recovered from community- and hospital-acquired urinary tract infections in turkey.the aim of this study was to evaluate the in vitro activities of antimicrobial agents including fosfomycin tromethamine against gram-negative isolates recovered from urine samples.201323742831
prevalence of quinolone resistance determinant qnra6 among broad- and extended-spectrum beta-lactam-resistant proteus mirabilis and morganella morganii clinical isolates with sul1-type class 1 integron association in a tunisian hospital.the aim of this study was to investigate the prevalence and the emergence of plasmid-mediated quinolone resistance among broad-spectrum beta-lactam-resistant proteus mirabilis and morganella morganii clinical isolates recovered in the military hospital in tunisia.201323746343
adverse effects of members of the enterobacteriaceae family on boar sperm quality.semen samples collected in 2012 from 1785 boars belonging to five different breeds were recruited from the quality control laboratory of magapor sl, spain. these samples came from 43 boar studs and resulted from diluting the ejaculates in commercial semen extenders. evaluation of the semen sample characteristics (color, smell, ph, osmolality, concentration, motility of sperm cells, agglutination, acrosome integrity, short hypoosmotic swelling test, and abnormal forms) revealed that they met the ...201323827823
more on peritonitis by morganella morganii. 201523843599
comparison of in vitro efficacy of ertapenem, imipenem and meropenem in the infections caused by the enterobacteriaceae strains family.the members of the bacterial enterobacteriaceae family play an important role in the aetiology of many hospital infections. some of them are able to produce β-lactamase, an enzyme which induces the resistance of those bacteria to penicillins, cephalosporins and, in severe infections, to penicillins with β-lactamase inhibitors. in this situation, the carbapenems become the drugs of choice. the objective of this study was to analyse the in vitro efficacy of three carbapenems: ertapenem, imipenem a ...201423877897
prevalence of community acquired urinary tract infections in silchar medical college, assam, india and its antimicrobial susceptibility profile.background: this study was conducted to know the rate of prevalence of community acquired uti infections in both males and females, assam, north-east india. materials and methods: a 50 μl of urine sample from each of the subjects was streaked on cystine lactose electrolyte-deficient agar medium. the plates were incubated overnight at 37°c. pure, isolated colonies of each type was separately cultured and characterized. the susceptibility of the clinical isolates to routinely prescribed antibiotic ...201223897522
[effect of colistin consumption and prevalence of colistin-resistant bacteria].recently, there has been a renaissance of the use of the antibiotic colistin resulting from increasing resistance of bacterial pathogens, particularly in intensive care patients. the study aimed at assessing the impact of colistin consumption on the prevalence of colistin-resistant bacteria in the university hospital olomouc (uho).201323965814
in vitro activity of ceftaroline-avibactam against gram-negative and gram-positive pathogens isolated from patients in canadian hospitals from 2010 to 2012: results from the canward surveillance study.the in vitro activities of ceftaroline-avibactam, ceftaroline, and comparative agents were determined for a collection of bacterial pathogens frequently isolated from patients seeking care at 15 canadian hospitals from january 2010 to december 2012. in total, 9,758 isolates were tested by using the clinical and laboratory standards institute (clsi) broth microdilution method (document m07-a9, 2012), with mics interpreted by using clsi breakpoints (document m100-s23, 2013). ceftaroline-avibactam ...201323979759
heat resistance of histamine-producing bacteria in irradiated tuna loins.consumption of foods high in biogenic amines leads to an illness known as histamine, or scombrotoxin, poisoning. the illness is commonly associated with consumption of fish with high levels of histamine ( $ 500 ppm). the objective of this study was to determine and compare the heat resistance of five histamine-producing bacteria in irradiated albacore tuna loins. heat-resistance parameters (d- and z-values) were determined for morganella morganii, raoultella planticola, hafnia alvei, and enterob ...201323992506
antibacterial activities of multi drug resistant myroides odoratimimus bacteria isolated from adult flesh flies (diptera: sarcophagidae) are independent of metallo beta-lactamase gene.sarcophagidae) are well known cause of myiasis and their gut bacteria have never been studied for antimicrobial activity against bacteria. antimicrobial studies of myroides spp. are restricted to nosocomial strains. a gram-negative bacterium, myroides sp., was isolated from the gut of adult flesh flies (sarcophaga sp.) and submitted to evaluation of nutritional parameters using biolog gn, 16s rrna gene sequencing, susceptibility to various antimicrobials by disc diffusion method and detection of ...200824031236
[a case of septic shock and multiple organ injury induced by urinary tract infection with morganella morganii subsp. morganii]. 201324059431
morganella morganii pericarditis in a patient with multiple myeloma.purulent pericarditis caused by morganella morganii is extremely rare. we report herein a case of a 61-year-old man who presented with chest pain and dyspnea fourteen days after chemotherapy for multiple myeloma. echocardiogram and computed tomography revealed a massive pericardial effusion and associated cardiac tamponade. pericardiocentesis was performed. pericardial fluid was found to be purulent, and morganella morganii was isolated from the fluid. the patient was successfully treated with a ...201324106621
intratesticular abscess in a solitary testicle: the case for testicle sparing management.we present the case of a 24-year-old immunocompromised man with an intratesticular abscess. the patient presented with one week of left scrotal pain and swelling. workup included scrotal ultrasonography which revealed a large fluid collection within the tunica albuginea of the left testis. surgical exploration of the left testis evacuated a significant amount of purulent fluid. the residual viable testicular parenchyma was salvaged. intraoperative cultures grew morganella morganii. follow-up ult ...201324198833
sequencing, characterization, and gene expression analysis of the histidine decarboxylase gene cluster of morganella morganii.the histidine decarboxylase gene cluster of morganella morganii dsm30146(t) was sequenced, and four open reading frames, named hdct1, hdc, hdct2, and hisrs were identified. two putative histidine/histamine antiporters (hdct1 and hdct2) were located upstream and downstream the hdc gene, codifying a pyridoxal-p dependent histidine decarboxylase, and followed by hisrs gene encoding a histidyl-trna synthetase. this organization was comparable with the gene cluster of other known gram negative bacter ...201424241330
oral flora of python regius kept as pets.this study was aimed at evaluating the oral bacterial flora of 60 python regius kept as pets by culture and biochemical methods. all isolates were also submitted to antimicrobial susceptibility testing using the disc diffusion method. the oral cavity of snakes sampled harboured a wide range of gram-negative bacteria mainly constituted by pseudomonas spp., morganella morganii, acinetobacter calcoaceticus, aeromonas hydrophila, but also by salmonella spp. staphylococcus spp. was the commonest gram ...201424383854
inhibitory effects of brown algae extracts on histamine production in mackerel muscle via inhibition of growth and histidine decarboxylase activity of morganella morganii.this study was performed to investigate the inhibitory effects of brown algae extracts on histamine production in mackerel muscle. first, antimicrobial activities of brown algae extracts against morganella morganii were investigated using a disk diffusion method. an ethanol extract of ecklonia cava (ecee) exhibited strong antimicrobial activity. the minimum inhibitory concentration (mic) of ecee was 2 mg/ml. furthermore, the brown algae extracts were examined for their ability to inhibit crude h ...201424394193
[antibiotic and sulfonamide sensitivity of proteus morganii]. 201424545240
characterization of the digestive tract microbiota of hirudo orientalis (medicinal leech) and antibiotic resistance profile.there are at least three distinct european leech species used medicinally: hirudo medicinalis, h. orientalis, and h. verbana. infection caused by leech microbiota is the most widely reported complication. few studies have reported the culturable and unculturable bacteria and examined the antibiotic resistances in h. orientalis.201424572887
trends of isolation of intrinsically resistant to colistin enterobacteriaceae and association with colistin use in a tertiary hospital.the objective of this investigation was to evaluate the association between colistin consumption and the isolation of intrinsically resistant to colistin enterobacteriaceae (irce) in a university hospital in crete, greece. the database of the microbiological laboratory was reviewed retrospectively during 2006-2010. all positive cultures for irce were retrieved. we assessed the total consumption of colistin in medical, surgical, and intensive care units (icus). a total of 1,304 single-patient irc ...201424798249
detection of food spoilage and pathogenic bacteria based on ligation detection reaction coupled to flow-through hybridization on membranes.traditional culturing methods are still commonly applied for bacterial identification in the food control sector, despite being time and labor intensive. microarray technologies represent an interesting alternative. however, they require higher costs and technical expertise, making them still inappropriate for microbial routine analysis. the present study describes the development of an efficient method for bacterial identification based on flow-through reverse dot-blot (ft-rdb) hybridization on ...201424818128
[purification and characterization of esterase from morganella morganii zjb-09203].enantioselective hydrolysis of 2-carboxyethyl-3-cyano-5-methylhexanoic acid (cnde) is the key step in chemoenzymatic synthesis of pregabalin. we purified an intracellular carboxyl esterase from morganella morganii zjb-09203, which exhibited high enantioselectivity and activity towards cnde. the carboxyl esterase was purified to electrophoretic homogeneity by ammonium sulfate fraction precipitation, phenyl sepharose 6 ff hydrophobic interaction chromatography, anion exchange with deae sephadex a- ...201424818487
protein content analysis and antimicrobial activity of the crude venom of montivipera bornmuelleri; a viper from lebanon.viperidae snakes venoms represent a source of efficient bioactive components that have already led to the development of several new drugs. in this work, we analyzed the protein content of the montivipera bornmuelleri crude venom using lc-esi-ms, sephadex g-75 gel filtration and sds-page and demonstrated the presence of proteins with molecular masses corresponding to metalloprotease iii, serine-protease and pla2 in three fractions collected after gel filtration. equally, we examined the antimicr ...201424853875
emergence of ndm-1-producing enterobacteriaceae in porto alegre, brazil.to evaluate the emergence of new delhi metallo-β-lactamase 1 (ndm-1)-producing enterobacteriaceae isolates in brazil.201424857802
role of the bacterial vaccine solco-urovac® in treatment and prevention of recurrent urinary tract infections of bacterial origin.urinary tract infections (uti) represent a serious medical problem with considerably high rate of morbidity. recurrent episodes of urinary tract infection (ruti) may commonly develop in the way of relapse or reinfection. for patients, ruti is always associated with costs, discomfort and decreased quality of life. standard treatment of ruti is through antibiotics and usually such treatment is required repeatedly. repeat course of antibiotics leads to increase of resistance of uropathogenic strain ...201425020163
effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content.the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ...201325049913
genome analysis of ndm-1 producing morganella morganii clinical isolate.to analyze the resistome and virulence genes of morganella morganii f675, a multidrug-resistant clinical isolate using whole genome sequencing (wgs).201425081858
type ii and type iv topoisomerase mutations in clinical isolates of morganella morganii harbouring the qnrd gene.the aim of this study was to show the emergence of the qnrd gene among fluoroquinolone-resistant morganella morganii isolate. the occurrence of mutations in dna gyrase (gyra and gyrb) and topoisomerase iv (parc,pare) genes was also investigated in this strain.201425106550
clinical manifestations and prognostic factors of morganella morganii bacteremia.although morganella morganii causes a variety of clinical infections, there are limited studies on m. morganii bacteremia after the year 2000. a total of 109 patients with m. morganii bacteremia at a medical center in taiwan from 2003 to 2012 were studied. among them, 30.3 % had polymicrobial bacteremia and 75.2 % had community-acquired infection. the most common underlying diseases were hypertension (62.4 %) and diabetes mellitus (38.5 %). the urinary tract (41.3 %) was the major portal of entr ...201525107625
characterization of a novel morganella morganii bacteriophage fsp1 isolated from river water.morganella morganii has been identified as a causative agent of opportunistic infections and histamine poisoning. bacteriophage is a virus and has recently been considered an alternative agent to antibiotics for the control of bacteria that have developed antibiotic resistance. in this study, a novel m. morganii bacteriophage isolated from river water was characterized. the isolated phage, termed fsp1, was purified by polyethylene glycol precipitation followed by cesium chloride density-gradient ...201425168469
evaluation of a modified cefsulodin-irgasan-novobiocin agar for isolation of yersinia spp.y. enterocolitica and y. pseudotuberculosis are important food borne pathogens. however, the presence of competitive microbiota makes the isolation of y. enterocolitica and y. pseudotuberculosis from naturally contaminated foods difficult. we attempted to evaluate the performance of a modified cefsulodin-irgasan-novobiocin (cin) agar in the differentiation of y. enterocolitica from non-yersinia species, particularly the natural intestinal microbiota. the modified cin enabled the growth of y. ent ...201425170941
solitary large intestinal diverticulitis in leatherback turtles (dermochelys coriacea).leatherback sea turtles are globally distributed and endangered throughout their range. there are limited data available on disease in this species. initial observations of solitary large intestinal diverticulitis in multiple leatherbacks led to a multi-institutional review of cases. of 31 subadult and adult turtles for which complete records were available, all had a single exudate-filled diverticulum, as large as 9.0 cm in diameter, arising from the large intestine immediately distal to the il ...201525239052
ctx-m-15-producing morganella morganii from hôpital principal de dakar, senegal.we report the detection and molecular characterization of extended spectrum β-lactamases in a series of 112 clinical isolates of enterobacteriaceae from the hôpital principal de dakar, senegal, including five ctx-m-15-producing morganella morganii isolates, which are reported for the first time in this country.201425356341
effects of breakpoint changes on carbapenem susceptibility rates of enterobacteriaceae: results from the sentry antimicrobial surveillance program, united states, 2008 to 2012.in the absence of clinical resistance, breakpoints for many antimicrobial agents are often set high. clinical failures following use of the agents over time requires re-evaluation of breakpoints. this is based on patient response, pharmacokinetic/pharmacodynamic information and in vitro minimal inhibitory concentration data. data from the sentry antimicrobial surveillance program has shown that clinical and laboratory standards institute breakpoint changes for carbapenems that occurred between 2 ...201425371693
morganella morganii causing abscess over the anterior chest wall- a case report.a 17-year-old female college student presented with recurrent abscess over the anterior chest wall since one and half year. morganella morganii was isolated from the aspirated pus. patient was started on oral ciprofloxacin and the lesion resolved in two weeks.201425386434
sepsis caused by new delhi metallo-β-lactamase (blandm-1) and qnrd-producing morganella morganii, treated successfully with fosfomycin and meropenem: case report and literature review.the objective of this study was to describe the microbiological characteristics of an extensively drug-resistant (xdr) isolate of morganella morganii obtained from a patient with sepsis of urinary origin and to describe the patient's clinical characteristics. we further aimed to perform a literature review of the situation in latin america regarding gram-negative bacillus (gnb) carriers of new delhi metallo-β-lactamase (ndm-1) and qnr genes and current reports on the treatment of infections caus ...201525447717
countrywide spread of oxa-48 carbapenemase in lebanon: surveillance and genetic characterization of carbapenem-non-susceptible enterobacteriaceae in 10 hospitals over a one-year period.to detect, characterize, and assess the genetic clonality of carbapenem-non-susceptible enterobacteriaceae in 10 lebanese hospitals in 2012.201425449248
enterobacteria identification and detection of diarrheagenic escherichia coli in a port complex.the port complex of maranhão (pcm) is the second largest port complex in brazil, receiving ships with large volumes of ballast water. to evaluate the microbiological quality of its waters, physicochemical parameters (ph and salinity), the number of coliforms (thermotolerants and totals), and the presence of enterobacterias and diarrheagenic escherichia coli strains were analyzed. in order to identify the presence of e. coli virulence genes target regions of the stx, elt, est, aggr, cvd432, ipah ...201425477930
dha-1 plasmid-mediated ampc β-lactamase expression and regulation of klebsiella pnuemoniae isolates.the present study aimed to investigate the regulatory mechanism of the ampc enzyme by analyzing the construction and function of ampcr, ampe and ampg genes in the dhahran (dha)‑1 plasmid of klebsiella pneumoniae (k. pneumoniae). the production of ampc and extended‑spectrum β‑lactamase (esbl) were determined following the cefoxitin (fox) inducing test for ampc, preliminary screening and confirmation tests for esbl in 10 dha‑1 plasmid ampc enzymes of k. pneumoniae strains. ampcr, ampd, ampe and am ...201525483576
the structure of the morganella morganii lipopolysaccharide core region and identification of its genomic loci.the core region of the lipopolysaccharide of morganella morganii serotype o:1ab was obtained by hydrolysis of the lps and studied by 2d nmr, esi ms, and chemical methods. its structure was highly homologous to those from the two major members of the same proteeae tribe, proteus mirabilis and providencia alcalifaciens, and analysis of the m. morganii genome disclosed that the loci for its outer core, lipid a and ara4n moieties are similarly conserved.201525498024
emergence of new sequence type oxa-48 carbapenemase-producing enterobacteriaceae in kuwait.the aim of this study was to investigate the infections due to oxa-48 carbapenemase-producing bacteria in tertiary hospitals in kuwait (september 2011 to april 2013) and to determine the sequence types (sts) of the corresponding isolates. eleven oxa-48 carbapenemase-producing enterobacteriaceae isolates were recovered from patients treated in nine different hospitals in kuwait. susceptibility testing to eighteen antibiotics was done using the e-test. pcr assays were performed for the detection o ...201525551428
structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.the structure of the oligosaccharide repeating units of endotoxins from gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria. detailed examination of the cross-reactions of three enterobacterial serotypes, proteus morganii o34, escherichia coli o111, and salmonella enterica sv. adelaide o35, was performed using sensitive tests (elisa, immunoblotting). fine differences between the endotoxins of the bacteria were detected using silver staining of sds-page ...201525630395
a metaproteomics approach to elucidate host and pathogen protein expression during catheter-associated urinary tract infections (cautis).long-term catheterization inevitably leads to a catheter-associated bacteriuria caused by multispecies bacterial biofilms growing on and in the catheters. the overall goal of the presented study was (1) to unravel bacterial community structure and function of such a uropathogenic biofilm and (2) to elucidate the interplay between bacterial virulence and the human immune system within the urine. to this end, a metaproteomics approach combined with in vitro proteomics analyses was employed to inve ...201525673765
hemorrhagic stomatitis in a natural hybrid of vipera ammodytes × vipera berus due to inappropriate substrate in terrarium.a natural hybrid of vipera ammodytes × vipera berus was presented having low body weight, seizures and generalized swelling of the cephalic region. based on the history of the case and clinical examination, hemorrhagic stomatitis of traumatic origin was diagnosed. the snake was kept in a terrarium with wood chips as a substrate, and the material had induced trauma in the oral mucosa which was further complicated with salmonella arizonae and morganella morganii co-infection, abscessation and oste ...201525715871
necrotizing soft tissue infection following a peripheral bypass.necrotizing soft tissue infection (nsti), formerly referred to as necrotizing fasciitis, is a rare but serious postoperative complication. nsti following arterial bypass is seen only once in the literature (for a coronary artery bypass) and is not mentioned following peripheral bypass. although surgical site infections have been studied extensively, there are limited published data on postoperative nsti and no data for nsti following peripheral arterial bypass.201525733218
in vitro activity of ceftazidime, ceftaroline and aztreonam alone and in combination with avibactam against european gram-negative and gram-positive clinical isolates.recent clinical isolates of key gram-negative and gram-positive bacteria were collected in 2012 from hospitalised patients in medical centres in four european countries (france, germany, italy and spain) and were tested using standard broth microdilution methodology to assess the impact of 4 mg/l avibactam on the in vitro activities of ceftazidime, ceftaroline and aztreonam. against enterobacteriaceae, addition of avibactam significantly enhanced the level of activity of these antimicrobials. mi ...201525748553
development of a real-time pcr method coupled with a selective pre-enrichment step for quantification of morganella morganii and morganella psychrotolerans in fish products.histamine fish poisoning is common and due to toxic concentrations of histamine often produced by gram-negative bacteria in fin-fish products with a high content of the free amino acid histidine. the genus morganella includes two species previously reported to cause incidents of histamine fish poisoning. morganella morganii and morganella psychrotolerans are both strong producer of histamine. however, little is known about the occurrence and critical stages for fish contamination with these bact ...201525791250
war wound treatment complications due to transfer of an incn plasmid harboring bla(oxa-181) from morganella morganii to ctx-m-27-producing sequence type 131 escherichia coli.a 22-year-old male developed a recurrent sacral abscess associated with embedded shrapnel following a blast injury. cultures grew extended-spectrum β-lactamase (esbl)-producing, carbapenem-susceptible escherichia coli. ertapenem was administered, but the infection recurred after each course of antibiotics. initial surgical interventions were unsuccessful, and subsequent cultures yielded e. coli and morganella morganii, both nonsusceptible to carbapenems. the isolates were carba np test negative, ...201525870058
conjunctival flora of clinically normal and diseased turtles and tortoises.in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises.201525889261
[new metalloendopeptidase of morganella morganii zm].proteolytic activity which is inhibited in the presence of o-phenanthroline was found in m. morganii zm. intracellular proteases of m. morganii zm unlimited split musculoskeletal actin in contrast to grimelysin. several proteolitic proteins of m. morganii zm cells were identified by zymography with gelatin. metalloproteinase of m. morganii zm cell lysate was purified by hydrophobic chromatography fractionation. the molecular weight of the protein was 35 kda.201525895364
molecular docking studies of the antitumoral activity and characterization of new chalcone.phytochemical investigation of azorella madreporica led to the isolation of four known compounds and an unknown chalcone. the structure of the new compound was identified by spectroscopy, including two-dimensional nmr techniques and comparison with published spectral data. the antioxidant activity of chalcone (compound 1) was measured using the 1,2-diphenyl-2-picryl-hydrazyl (dpph) free radical scavenging assay, and the bioactivity was evaluated against five bacteria (mycobacterium smegmatis atc ...201525915607
antimicrobial activity and chemical composition of the essential oils of portuguese foeniculum vulgare fruits.the aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of portugal. composition of essential oils was established by gas chromatography-flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms) analysis. the obtained yields of the essential oils were found to vary greatly in the range ...201525973507
draft whole-genome sequence of morganella morganii serotype o:1ab.morganella morganii is a facultative pathogen of humans, causing urinary tract and postsurgical infections. here, we report a high-quality draft assembly of the o:1ab serotype.201525999571
identification of dha-23, a novel plasmid-mediated and inducible ampc beta-lactamase from enterobacteriaceae in northern taiwan.ampc β-lactamases are classified as amber class c and bush group 1. ampc β-lactamases can hydrolyze broad and extended-spectrum cephalosporins, and are not inhibited by β-lactamase inhibitors such as clavulanic acid. this study was conducted to identify dha-23, a novel plasmid-mediated and inducible ampc β-lactamase obtained from enterobacteriaceae.201525999942
molecular characteristics of extended-spectrum cephalosporin-resistant enterobacteriaceae from humans in the community.to investigate the molecular characteristics of extended-spectrum cephalosporin (esc)-resistant enterobacteriaceae collected during a cross-sectional study examining the prevalence and risk factors for faecal carriage of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in humans living in areas with high or low broiler density.201526029910
increased prevalence of carbapenem resistant enterobacteriaceae in hospital setting due to cross-species transmission of the bla ndm-1 element and clonal spread of progenitor resistant strains.this study investigated the transmission characteristics of carbapenem-resistant enterobacteriaceae (cre) strains collected from a hospital setting in china, in which consistent emergence of cre strains were observable during the period of may 2013 to february 2014. among the 45 cre isolates tested, 21 (47%) strains were found to harbor the bla ndm-1 element, and the rest of 24 cre strains were all positive for bla kpc-2. the 21 bla ndm-1-borne strains were found to comprise multiple enterobacte ...201526136735
enterobacter strains might promote colon cancer.many studies have been performed to determine the interaction between bacterial species and cancer. however, there has been no attempts to demonstrate a possible relationship between enterobacter spp. and colon cancer so far. therefore, in the present study, it is aimed to investigate the effects of enterobacter strains on colon cancer. bacterial proteins were isolated from 11 enterobacter spp., one morganella morganii, and one escherichia coli strains, and applied onto ncm460 (incell) and crl17 ...201526143056
prevalence and characterization of high histamine-producing bacteria in gulf of mexico fish species.recent developments in detection and enumeration of histamine-producing bacteria (hpb) have created powerful molecular-based tools to better understand the presence of spoilage bacteria and conditions, resulting in increased risk of scombrotoxin fish poisoning. we examined 235 scombrotoxin-forming fish from the gulf of mexico for the presence of high hpb. photobacterium damselae subsp. damselae was the most prevalent hpb (49%), followed by morganella morganii (14%), enterobacter aerogenes (4%), ...201526197285
low-level antimicrobial resistance of enterobacteriaceae isolated from the nares of pig-exposed persons.extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esbl-e) have recently emerged in livestock and humans. therefore, this study assessed the carriage of enterobacteriaceae in the anterior nares and associated antimicrobial resistance in pig-exposed persons. nasal swabs were enriched in non-selective broth and then plated on macconkey and esbl-selective agars. species was confirmed by matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry (maldi-tof ms). anti ...201626219671
mmosti restriction endonuclease, isolated from morganella morganii infecting a tropical moth, actias selene, cleaving 5'-|ccngg-3' sequences.a type ii restriction endonuclease, mmosti, from the pathogenic bacterium morganella morganii infecting a tropical moth, actias selene, has been detected and biochemically characterized, as a potential etiological differentiation factor. the described rease recognizes interrupted palindromes, i.e., 5'-ccngg-3' sequences and cleaves dna leaving 5-nucleotide (nt) long, single-stranded (ss), 5'-cohesive ends, which was determined by three complementary methods: (i) cleavage of custom and standard d ...201626280518
a rapidly progressing, deadly disease of actias selene (indianmoonmoth) larvae associated with a mixed bacterial and baculoviral infection.the outbreak of an infectious disease in captive-bred lepidoptera can cause death of all the caterpillars within days. a mixed baculoviral-bacterial infection observed among actias selene (hubner 1807), the indian moon moth (insecta: lepidoptera: saturniidae), larvae was characterized and followed by a photographic documentation of the disease progression. the etiological agents were determined using mass spectrometry and polymerase chain reaction (pcr). it appeared that the disease was caused b ...201526333395
draft genome sequence of a diarrheagenic morganella morganii isolate.this is a report of the whole-genome draft sequence of a diarrheagenic morganella morganii isolate from a patient in michigan, usa. this genome represents an important addition to the limited number of pathogenic m. morganii genomes available.201526450735
activity of fosfomycin and comparison of several susceptibility testing methods against contemporary urine isolates.fosfomycin is recommended as first-line treatment for acute uncomplicated cystitis in women. it has demonstrated in vitro activity against a variety of pathogens; however, a paucity of data are available from the usa. we determined the susceptibility of a collection of urine isolates to fosfomycin and compared multiple methods of susceptibility testing. consecutive non-duplicate enterobacteriaceae, enterococci and pseudomonas aeruginosa isolates were collected from the clinical microbiology labo ...201526498988
optical scatter patterns facilitate rapid differentiation of enterobacteriaceae on chromagar™ orientation medium.enterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. enterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as chromagar(tm) orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production. here, we investigated the ability of the laser optical sensor, bardot (bacterial rapid detection usin ...201526503189
neonatal sulfhemoglobinemia and hemolytic anemia associated with intestinal morganella morganii.sulfhemoglobinemia is a rare disorder characterized by the presence of sulfhemoglobin in the blood. it is typically drug-induced and may cause hypoxia, end-organ damage, and death through oxygen deprivation. we present here a case of non-drug-induced sulfhemoglobinemia in a 7-day-old preterm infant complicated by hemolytic anemia. microbiota compositional analysis of fecal samples to investigate the origin of hydrogen sulphide revealed the presence of morganella morganii at a relative abundance ...201526553186
multiple liver abscesses due to morganella morganii. 201526574995
follow-up after infants younger than 2 months of age with urinary tract infection in southern israel: epidemiologic, microbiologic and disease recurrence characteristics.the timing of most recurrences after neonatal urinary tract infection is during the first year of life, with peak incidence 2-6 months after the initial infection. information on the microbiologic characteristics of recurrent urinary tract infection episodes in relation to the microbiology of the initial episodes is limited.201626607682
[antimicrobial resistance monitoring of gram-negative bacilli isolated from 15 teaching hospitals in 2014 in china].to investigate the current situation of antimicrobial resistance of nosocomial gram-negative bacilli in 2014 in china.201526675021
morganella morganii peritonitis associated with continuous ambulatory peritoneal dialysis (capd) after colonoscopy.a 79-year-old man on continuous ambulatory peritoneal dialysis (capd) developed abdominal pain and cloudy peritoneal fluid two days after colonoscopy that revealed multiple diverticula. the white blood cell count was 9,000 cells/μl, c-reactive protein level was 6.86 mg/dl, and the white blood cell count of the peritoneal fluid was 7,800 cells/μl, suggesting acute peritonitis. empiric therapy consisting of cefazolin and ceftazidime slowly improved the patient's symptoms. the initial microbiologic ...201626781017
evidence of antibacterial activities in peptide fractions originating from snow crab (chionoecetes opilio) by-products.antibacterial peptide fractions generated via proteolytic processing of snow crab by-products exhibited activity against gram-negative and gram-positive bacteria. among the bacterial strains tested, peptide fractions demonstrated inhibitory activity against the gram-negative bacteria such as aeromonas caviae, aeromonas hydrophila, campylobacter jejuni, listonella anguillarum, morganella morganii, shewanella putrefasciens, vibrio parahaemolyticus and vibrio vulnificus and against a few gram-posit ...201026781242
histamine development and bacterial diversity in microbially-challenged tonggol (thunnus tonggol) under temperature abuse during canning manufacture.histamine formation and bacteriological changes caused by temperature abuse commonly occurring in the manufacturing process of standard canned tuna was assessed in microbiologically challenged tonggol (thunnus tonggol). the in situ challenge was performed by water-soaking at 26-28 °c for 7 h to ensure the multiplication and active phase of fish microflora. right after pre-cooking to back-bone temperature (bbt) of 50-52 °c, histamine dropped to 5.17 ± 2.71 ppm, and slowly reached 6.84 ± 1.69 ppm ...201526787946
development of molecular approach based on pcr assay for detection of histamine producing bacteria.histamine fish poisoning becomes highly concern not only in public health but also economic aspect. histamine is produced from histidine in fish muscles by bacterial decarboxylase enzyme. several techniques have been developed to determine the level of histamine in fish and their products but the effective method for detecting histamine producing bacteria is still required. this study was attempted to detect histamine producing bacteria by newly developed pcr condition. histamine producing bacte ...201526787983
septic shock induced by bacterial prostatitis with morganella morganii subsp. morganii in a posttransplantation patient.bacterial infection is a common complication after hematopoietic stem cell transplantation (hsct). morganella morganii is ubiquitous gram-negative facultative anaerobe, which may cause many kinds of opportunistic infection. herein we report a case of a 55-year-old man who presented with frequent urination, urgency, and mild pain that comes and goes low in the abdomen and around the anus. the patient had a medical history of chronic prostatitis for 4 years. he received hla-matched sibling allo-hs ...201526798544
[morpho-functional characteristic of enterobacteria adhesive activity].study adhesive activity of enterobacteria in the model of hemagglutination reaction with animal and avian erythrocytes and clarify structures responsible for adhesion in enterobacteria.201626829846
[the great imitator. case report].authors present two patients suffering from renal tuberculosis, which caused differential diagnostic problems. the first patient was examined because of fever and left flank pain. computed tomography revealed renal shrinkage on the left side. retrograde pyelography demonstrated ureteric stricture and dilated calices. urine culture showed mycobacterium tuberculosis. two months after initiation of the antituberculotic therapy nephrectomy was performed. the second patient was referred to the depart ...201626895803
inhibition of morganella morganii histidine decarboxylase activity and histamine accumulation in mackerel muscle derived from filipendula ulumaria extracts.filipendula ulmaria, also known as meadowsweet, is an herb; its extract was examined for the prevention of histamine production, primarily that caused by contaminated fish. the efficacy of meadowsweet was assessed using two parameters: inhibition of morganella morganii histidine decarboxylase (hdc) and inhibition of histamine accumulation in mackerel. ellagitannins from f. ulmaria (rugosin d, rugosin a methyl ester, tellimagrandin ii, and rugosin a) were previously shown to be potent inhibitors ...201626939657
bacteriology of naja atra snakebite wound and its implications for antibiotic therapy.a total of 112 cases of naja atra envenomation were examined at two referring hospitals: taichung veterans general hospital in central taiwan and taipei veterans general hospital (vgh-tp) in northern taiwan. overall, 77% (86/112) of cases developed clinically suspected wound infections and 54% (61/112) required surgery secondary to tissue necrosis, finger or toe gangrene, and/or necrotizing fasciitis. morganella morganii was the most abundant gram-negative bacterial strain isolated from bite wou ...201626976881
epidemiology and antimicrobial susceptibility profiles of pathogens causing urinary tract infections in the asia-pacific region: results from the study for monitoring antimicrobial resistance trends (smart), 2010-2013.a total of 9599 isolates of gram-negative bacteria (gnb) causing urinary tract infections (utis) were collected from 60 centres in 13 countries in the asia-pacific region from 2010-2013. these isolates comprised enterobacteriaceae species (mainly escherichia coli, klebsiella pneumoniae, proteus mirabilis, klebsiella oxytoca, enterobacter cloacae and morganella morganii) and non-fermentative gnb species (predominantly pseudomonas aeruginosa and acinetobacter baumannii). in vitro susceptibilities ...201627005459
sonication of abdominal drains: clinical implications of quantitative cultures for the diagnosis of surgical site infection.the use of drains in patients undergoing abdominal surgery has been a subject of debate for several decades. in this paper, the usefulness of quantitative cultures of sonicated abdominal drains for diagnosing surgical site of infection (ssi) and the association between culture results with patient outcome is evaluated.201627046739
infected cephalohematomas and underlying osteomyelitis: a case-based review.cephalohematomas are relatively common sub-periosteal collections of blood that tend to resolve spontaneously without treatment. rarely, they become infected and can be associated with underlying osteomyelitis, meningitis, or sepsis. common pathogens include escherichia coli and staphylococcus species.201627066799
a severe morganella morganii endophthalmitis; followed by bacteremia.morganella morganii is rarely isolated from nosocomial infections. however, postoperative infections due to morganella spp. were documented in literature and eye involvements of the infections usually result in severe sequels. we present a severe case infection, which was caused by m. morganii subsp. morganii, firstly appearing as conjunctivitis and complicated by bacteremia. the infectious agent isolated from both conjunctival and consecutive blood cultures. identification and antimicrobial sus ...201627092227
multi-typing of enterobacteria harboring lt and st enterotoxin genes isolated from mexican children.enterotoxigenic escherichia coli is the most common cause of diarrhea in children younger than 5 years in the developing world. we used 16s rrna gene sequencing, the biolog(®) system, and an amplified ribosomal dna restriction analysis (ardra) to identify 69 enterobacteria isolated from the feces of healthy children up to 12 years old and 54 enterobacteria isolated from stool samples obtained from children up to 5 years old with diarrhea from morelia, michoacán, mexico. in the diarrheic group, 1 ...201727169941
characterization of integrons and qnr genes in proteeae from a teaching hospital in china.proteeae isolates displaying multidrug-resistance (mdr) are the second most common causes of hospital-associated infections. the aim of this study was to screen class 1-3 integrons and plasmid-mediated quinolone resistance (pmqr) genes in proteeae isolates from the first affiliated hospital of the wenzhou medical university.201727220322
molecular investigation of extended-spectrum beta-lactamase genes and potential drug resistance in clinical isolates of morganella morganii.resistance to beta-lactam antibiotics has become more common in morganella morganii, which can cause of outbreaks of bacteremia and septicemia in postoperative patients.201727236395
morganella morganii bacteria produces phenol as the sex pheromone of the new zealand grass grub from tyrosine in the colleterial gland.costelytra zealandica (coleoptera: scarabeidae) is a univoltine endemic species that has colonised and become a major pest of introduced clover and ryegrass pastures that form about half of the land area of new zealand. female beetles were previously shown to use phenol as their sex pheromone produced by symbiotic bacteria in the accessory or colleterial gland. in this study, production of phenol was confirmed from the female beetles, while bacteria were isolated from the gland and tested for at ...201627352077
active surveillance scheme in three romanian hospitals reveals a high prevalence and variety of carbapenamase-producing gram-negative bacteria: a pilot study, december 2014 to may 2015.we report the findings of an active surveillance scheme for detection of asymptomatic carriers with carbapenemase-producing gram-negative bacteria (cp-gnb) in romanian hospitals. during a pilot study from december 2014 to may 2015, faecal cultures were screened in three hospitals (two large, one medium-size) for patients newly admitted to selected wards or inpatients transferred from other wards to an intensive-care unit. the study revealed a high prevalence of cp-gnb detected in 22/27 and 28/38 ...201627363583
in vitro activity of tigemonam against urinary tract pathogens.tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serrada marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory c ...198927416146
prevalence of extended spectrum beta-lactamase production among uropathogens in south mumbai and its antibiogram pattern.β-lactams are the most widely used group of antimicrobials. however, increasing resistance to these valuable drugs in uropathogens, mediated principally by β-lactamases, has become a major concern. the present study was undertaken to determine the prevalence of extended spectrum β-lactamase (esbl) producers in clinical isolates of urine specimens, collected from various healthcare centres across south mumbai. a total of 195 gram negative urine isolates were identified as pseudomonas aeruginosa ( ...201227418912
morganella morganii, a non-negligent opportunistic pathogen.morganella morganii belongs to the tribe proteeae of the enterobacteriaceae family. this species is considered as an unusual opportunistic pathogen that mainly causes post-operative wound and urinary tract infections. however, certain clinical m. morganii isolates present resistance to multiple antibiotics by carrying various resistant genes (such as blandm-1, and qnrd1), thereby posing a serious challenge for clinical infection control. moreover, virulence evolution makes m. morganii an importa ...201627421818
persistent infection with metallo-beta-lactamase and extended spectrum β-lactamase producer morganella morganii in a patient with urinary tract infection after kidney transplantation.organ transplant recipients under immunosuppressive therapy have a highly increased risk of acquiring unusual opportunistic infections. diagnosis of the etiology of infection may be difficult in clinical manifestations, which need further histological and biological investigations. here in we report, for the 1(st) time in the iran, a morganella morganii isolate harboring blavim, blactx-m, and blashv genes after kidney transplantation with persistent urinary infections.201727433071
clonal transmission of gram-negative bacteria with carbapenemases ndm-1, vim-1, and oxa-23/72 in a bulgarian hospital.we characterized 72 isolates with reduced susceptibility to carbapenems (50 acinetobacter spp., 13 proteus mirabilis, five escherichia coli, one morganella morganii, one enterobacter cloacae, one providencia rettgeri, and one pseudomonas aeruginosa) from a hospital in sofia, bulgaria. different β-lactamase genes were identified by polymerase chain reaction and sequencing. bacterial strain typing was performed by enzymatic macrorestriction and pulsed-field gel electrophoresis (pfge) typing as wel ...201727459019
severe chronic osteomyelitis caused by morganella morganii with high population diversity.a case of chronic osteomyelitis probably caused by morganella morganii, occurring over a period of 30 years, is reported. the organism was identified through a combination of sample culture, direct sequencing, and 16s rna gene amplicon sequencing. further whole-genome sequencing and population structure analysis of the isolates from the patient showed the bacterial population to be highly diverse. this case provides a valuable example of a long-term infection caused by an opportunistic pathogen, ...201627495005
biofilm in osteomyelitis caused by a rare pathogen, morganella morganii : a case report.morganella morganii is a member of enterobacteriaceae family, whose natural habitat is the human gastrointestinal tract. it rarely causes infection alone and is generally encountered in immunosuppressed patients. osteoarticular pathologies are not commonly observed with morganella morganii and infections by it have high mortality rate. biofilm colonization is a causative factor behind the chronicity and/or refractoriness of certain infections. biofilms colonize on inert medical devices, prosthes ...201627504288
characterization of complex, heterogeneous lipid a samples using hplc-ms/ms technique i. overall analysis with respect to acylation, phosphorylation and isobaric distribution.we established a new reversed phase-high performance liquid chromatography method combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry for the simultaneous determination and structural characterization of different lipid a types in bacteria (escherichia coli o111, salmonella adelaide o35 and proteus morganii o34) showing serological cross-reactivity. the complex lipid a mixtures (obtained by simple extraction and acid hydrolysis of the outer membrane lipopolys ...201627506631
can surgical need in patients with naja atra (taiwan or chinese cobra) envenomation be predicted in the emergency department?to investigate the clinical predictors and the aetiologies for surgery in patients with naja atra (taiwan or chinese cobra) envenomation.201627516567
architecture of class 1, 2, and 3 integrons from gram negative bacteria recovered among fruits and vegetables.the spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. to understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). although low levels of resistance have been detected, the bacter ...201627679611
infectious bacterial pathogens, parasites and pathological correlations of sewage pollution as an important threat to farmed fishes in egypt.this paper is a part of a multi-disciplinary research "application of decentralized on-site water treatment system in egypt for use in agriculture and producing safe fish and animal proteins". the project aimed to investigate the environmental impact of implementing sewage water before and after treatment using the effluent of the on-site decentralized japanese' johkasou system, in agriculture and producing fish protein. the aim is to establish such system in egypt to strengthen the sanitary con ...201627720545
in vivo acquisition of carbapenemase gene blakpc-2 in multiple species of enterobacteriaceae through horizontal transfer of insertion sequence or plasmid.objectives: current worldwide spread of carbapenem resistance in enterobacteriaceae constitutes a critical public health threat. this study aims to investigate how carbapenem resistance is acquired in enterobacteriaceae in patients during antimicrobial therapy. methods: clinical strains from the same anatomical site of the same patients that converted from carbapenem-susceptible to resistant during antimicrobial therapy and showed identical or similar pfge patterns were identified. the similarly ...201627818649
draft genomic analysis of an avian multidrug resistant morganella morganii isolate carrying qnrd1.morganella morganii is a commensal bacterium and opportunistic pathogen often present in the gut of humans and animals. we report the 4.3 mbp draft genome sequence of a m. morganii isolated in association with an escherichia coli from broilers in portugal that showed macroscopic lesions consistent with colisepticemia. the analysis of the genome matched the multidrug resistance phenotype and enabled the identification of several clinically important and potentially mobile acquired antibiotic resi ...201627826290
susceptibility patterns of uropathogens identified in hospitalised children with community-acquired urinary tract infections in thrace, greece.urinary tract infections (utis) are common infections associated with considerable morbidity and mortality, particularly in paediatric patients. the alarmingly increasing antimicrobial resistance of contemporary uropathogens in children necessitates the re-evaluation of antibiotic treatment. we evaluated uropathogens isolated from children hospitalised due to a community-acquired uti over a 5.5-year period in a university hospital of northern greece and their antibiotic susceptibility patterns. ...201527873675
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