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the clpb protein from campylobacter jejuni: molecular characterization of the encoding gene and antigenicity of the recombinant protein.the clpb heat-shock protein is necessary for the survival of escherichia coli cells upon sudden increase of temperature. using a pcr-based genomic walking method, the nucleotide sequence of a clpb homolog from campylobacter jejuni was determined. the clpb gene encodes a protein of 857 amino acid (aa) residues, with a predicted molecular mass of 95.3kda. alignment of the deduced aa sequence with other known bacterial clpb proteins revealed overall identity from 47% (e. coli) to 61% (helicobacter ...199910196475
serotyping of campylobacter jejuni based on heat-stable antigens: relevance, molecular basis and implications in pathogenesis. 199910196742
intermittent shedding of thermophilic campylobacters by sheep at pasture.the rates at which sheep on different types of pasture shed campylobacters in their faeces were measured over 12 months. overall, shedding of campylobacters at pasture was between a third and a half of the carriage rate (92%) of the intestines of sheep at slaughter. shedding was highest during saltmarsh grazing, followed by upland fell and farm grazing. the rate of shedding varied at different times of the year, with the highest rates (100%) coinciding with lambing, weaning, and movement onto ne ...199910196758
morphological changes of synchronized campylobacter jejuni populations during growth in single phase liquid culture.campylobacter jejuni strains demonstrate a variety of growth phase-linked distinct morphological forms when grown in liquid culture. the typical spiral form of the organism, evident during logarithmic phase, undergoes elongation during stationary phase before becoming coccoid via the formation of membrane blebs and budded forms in decline phase. cellular elongation and coccoid formation occurred despite the inhibition of protein synthesis and without a detectable change in the protein components ...199910196767
identification of a gene encoding an immuno-reactive membrane protein from campylobacter jejuni.a gene encoding a putative membrane protein has been identified from campylobacter jejuni nctc 11168 following an immuno-screen of a lambda zap ii genomic dna library with antiserum raised against glycine-extractable proteins. the nucleotide sequence of the entire genomic insert revealed six open reading frames, all but one of which have sequence homologues in the complete genome sequence of helicobacter pylori. the gene encoding the immuno-reactive protein was further identified by independent ...199910196775
isolation of helicobacter pylori genes that modulate urease activity.helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to nh3 and co2. we sought to identify h. pylori genes that modulate urease activity by constructing php8080, a plasmid which encodes both h. pylori urease and the nixa nickel transporter. escherichia coli se5000 and dh5alpha transformed with php8080 resulted in a high-level urease producer and a low-level urease producer, respectively. an h. pylori dna library was cotransformed into se5000 (php8080) and dh5alpha (php80 ...199910198012
campylobacter jejuni ii peritonitis in a ccpd patient: cure by oral clarithromycin. 199910201350
actinobacillus actinomycetemcomitans immunosuppressive protein is a member of the family of cytolethal distending toxins capable of causing a g2 arrest in human t cells.we have previously shown that actinobacillus actinomycetecomitans produces an immunosuppressive factor (isf) capable of impairing human lymphocyte function by perturbing cell cycle progression. we now report that isf is the product of the cdtb gene, one of three genes encoding the family of cytolethal distending toxins (cdt). the isf polypeptide exhibits >/=95% identity with hemophilus ducreyi cdtb protein and </=60% homology with escherichia coli or campylobacter jejuni cdtb. pretreatment of ph ...199910202019
predictive fluorescent amplified-fragment length polymorphism analysis of escherichia coli: high-resolution typing method with phylogenetic significance.the fluorescent amplified-fragment length polymorphism (faflp) assay potentially amplifies a unique set of genome fragments from each bacterial clone. it uses stringently hybridizing primers which carry a fluorescent label. precise fragment sizing is achieved by the inclusion of an internal size standard in every lane. therefore, a unique genotype identifier(s) can be found in the form of fragments of precise size or sizes, and these can be generated reproducibly. in order to evaluate the potent ...199910203470
serological discrimination of dogs infected with gastric helicobacter spp. and uninfected dogs.characterization of the humoral immune responses of people to helicobacter pylori infection has facilitated the investigation of the host response to bacterial virulence factors and the development of sensitive and specific diagnostic tests. dogs are commonly infected with gastric helicobacter spp., but the presence of multiple helicobacter spp. and possible coinfection in individual dogs have complicated serological evaluation. evaluation of the antigenic homology of helicobacter spp. revealed ...199910203471
cytolethal distending toxin genes in campylobacter jejuni and campylobacter coli isolates: detection and analysis by pcr.campylobacter jejuni produces a toxin called cytolethal distending toxin (cdt). knowledge of the prevalence and homogeneity of campylobacter sp. cdt genes is incomplete. in this work, we identified four pcr primer pairs that collectively amplified cdt genes in all of the c. jejuni and campylobacter coli strains tested. restriction analyses of the cdt pcr products showed clear differences between the cdt genes of these two species, yet there were few heterogeneities noted between members of the s ...199910203548
cloning, sequencing and molecular analysis of the campylobacter jejuni groesl bicistronic operon.the groesl bicistronic operon from the enteric pathogen campylobacter jejuni was cloned and sequenced. it consists of two orfs encoding proteins with molecular masses of 9.5 and 57.9 kda, which showed a high degree of homology to other bacterial groes and groel proteins. northern blot analysis suggested that the groesl operon is transcribed as a bicistronic mrna, and its steady-state level was markedly increased after temperature upshift. by primer extension assay, one potential transcription st ...199910206714
differentiation of campylobacter coli and c. jejuni by length and dna sequence of the 16s-23s rrna internal spacer region.the internal spacer region (isr) between the 16s and 23s rrna genes of campylobacter was investigated by pcr fragment length typing and dna sequencing of clinical and chicken wild-type isolates. pcr fragment length typing showed one fragment of 859 nt in length for the 12 strains of campylobacter coli investigated. thirty-six of the campylobacter jejuni subsp. jejuni strains possessed one fragment, which varied in size between 727 and 802 nt. three strains showed two fragments between 501 and 92 ...199910206715
the use of hipo, encoding benzoylglycine amidohydrolase (hippuricase), as a reporter of gene expression in campylobacter coli.a novel integrative promoter probe vector which utilizes the campylobacter jejuni hipo gene as a reporter of gene expression was developed as a genetic tool in campylobacter coli. the utility of the system was demonstrated by coupling expression of the hipo reporter to the promoters of flaa, flab and kata. subsequently, expression of these genes could be monitored accurately from chromosomally-borne transcriptional fusions using a simple non-destructive colorimetric assay. the system should serv ...199910212441
campylobacter jejuni diarrhea in north karnataka. 199810216571
the yersinia enterocolitica motility master regulatory operon, flhdc, is required for flagellin production, swimming motility, and swarming motility.the ability to move over and colonize surface substrata has been linked to the formation of biofilms and to the virulence of some bacterial pathogens. results from this study show that the gastrointestinal pathogen yersinia enterocolitica can migrate over and colonize surfaces by swarming motility, a form of cooperative multicellular behavior. immunoblot analysis and electron microscopy indicated that swarming motility is dependent on the same flagellum organelle that is required for swimming mo ...199910217774
high-resolution genomic fingerprinting of campylobacter jejuni and campylobacter coli by analysis of amplified fragment length polymorphisms.a method for high-resolution genomic fingerprinting of the enteric pathogens campylobacter jejuni and campylobacter coli, based on the determination of amplified fragment length polymorphism, is described. the potential of this method for molecular epidemiological studies of these species is evaluated with 50 type, reference, and well-characterised field strains. amplified fragment length polymorphism fingerprints comprised over 60 bands detected in the size range 35-500 bp. groups of outbreak s ...199910220884
pharmacokinetics of gatifloxacin and interaction with an antacid containing aluminum and magnesium.the pharmacokinetics of gatifloxacin (400 mg orally) and the influence of the antacid aluminum magnesium hydroxide (20 ml of maalox 70) on the bioavailability of gatifloxacin in 24 healthy volunteers were assessed. in an open, randomized, six-period crossover study, the volunteers received either gatifloxacin alone (treatments a and d); aluminum magnesium hydroxide concomitant with gatifloxacin (treatment c); or aluminum magnesium hydroxide 2 h before (treatment b), 2 h after (treatment e), or 4 ...199910223915
sequence analysis of the gyra and parc homologues of a wild-type strain of vibrio parahaemolyticus and its fluoroquinolone-resistant mutants.vibrio parahaemolyticus causes seafood-borne gastroenteritis in humans. it is particularly important in japan, where raw seafood is frequently consumed. fluoroquinolone is one of the current drugs of choice for treating patients infected by v. parahaemolyticus because resistant strains are rarely found. to study a possible fluoroquinolone resistance mechanism in this organism, nucleotide sequences that are homologous to known gyra and parc genes have been cloned from v. parahaemolyticus aq3815 a ...199910223929
pullulanase type i from fervidobacterium pennavorans ven5: cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme.the gene encoding the type i pullulanase from the extremely thermophilic anaerobic bacterium fervidobacterium pennavorans ven5 was cloned and sequenced in escherichia coli. the pula gene from f. pennavorans ven5 had 50.1% pairwise amino acid identity with pula from the anaerobic hyperthermophile thermotoga maritima and contained the four regions conserved among all amylolytic enzymes. the pullulanase gene (pula) encodes a protein of 849 amino acids with a 28-residue signal peptide. the pula gene ...199910224005
stability of related human and chicken campylobacter jejuni genotypes after passage through chick intestine studied by pulsed-field gel electrophoresis.the genomic stability of 12 campylobacter jejuni strains consisting of two groups of human and chicken isolates was studied by analysis of their pfge (pulsed-field gel electrophoresis) patterns after passage through newly hatched chicks' intestines. the patterns of smai, sali, and sacii digests remained stable after intestinal passage, except for those of two strains. one originally human strain, fb 6371, changed its genotype from ii/a (smai/sacii) to i/b. another strain, bti, originally isolate ...199910224037
distinct immunoglobulin class and immunoglobulin g subclass patterns against ganglioside gq1b in miller fisher syndrome following different types of infection.we studied serum antibodies against gangliosides gq1b and gm1 in 13 patients with miller fisher syndrome (mfs) and in 18 patients with guillain-barré syndrome (gbs) with cranial nerve involvement. anti-gq1b titers were elevated in all patients with mfs cases (immunoglobulin g [igg] > iga, igm), and in 8 of the 18 with gbs. lower frequencies of increased anti-gm1 titers were observed in mfs patients (3 of 13), as well as in gbs patients (5 of 18). during the course of mfs, anti-gq1b titers of all ...199910225903
identification of virulence genes of helicobacter pylori by random insertion mutagenesis.the complete genome of the gram-negative bacterial pathogen helicobacter pylori, an important etiological agent of gastroduodenal disease in humans, has recently been published. this sequence revealed that the putative products of roughly one-third of the open reading frames (orfs) have no significant homology to any known proteins. to be able to analyze the functions of all orfs, we constructed an integration plasmid for h. pylori and used it to generate a random mutant library in this organism ...199910225906
host cell death due to enteropathogenic escherichia coli has features of apoptosis.enteropathogenic escherichia coli (epec) is a cause of prolonged watery diarrhea in children in developing countries. the ability of epec to kill host cells was investigated in vitro in assays using two human cultured cell lines, hela (cervical) and t84 (colonic). epec killed epithelial cells as assessed by permeability to the vital dyes trypan blue and propidium iodide. in addition, epec triggered changes in the host cell, suggesting apoptosis as the mode of death; such changes included early e ...199910225923
mechanisms of internalization of staphylococcus aureus by cultured human osteoblasts.staphylococcus aureus is an important bone pathogen, and evidence shows that this organism is internalized by chick osteoblasts. here we report that s. aureus is internalized by human osteoblasts. internalization was inhibited by monodansylcadaverine and cytochalasin d and to a lesser extent by ouabain, monensin, colchicine, and nocodazole. we propose that internalization occurs via a receptor-mediated pathway, requiring the participation of cytoskeletal elements, principally actin.199910225942
study of the presence of campylobacter jejuni and c. coli in sand samples from four swiss chicken farms.chicken farms are frequently infected with campylobacter jejuni and campylobacter coli. the objective of the present study was to investigate environmental samples from chicken farms for the presence of c. jejuni and c. coli. every week between july and november 1997, three sand samples from the runs of four chicken farms were analyzed by culture and directly by polymerase chain reaction (pcr). these two detection methods were compared to each other. a total of 231 samples were tested. eleven sa ...199910229951
natural and experimental infections of arcobacter in poultry.arcobacter butzleri causes human enteritis and is frequently recovered from poultry carcasses. the purpose of this study was to determine 1) the natural distribution of a. butzleri in poultry and 2) its relative pathogenicity in experimentally infected poultry. cloacal samples (n = 407) were collected on four occasions from three flocks of chickens. overall, arcobacter spp. were recovered from 15% of the birds; a. butzleri was identified in 1% of cloacal samples. three experimental trials were c ...199910230906
a novel campylobacter jejuni two-component regulatory system important for temperature-dependent growth and colonization.campylobacter jejuni colonizes the intestines of domestic and wild animals and is a common cause of human diarrheal disease. we identified a two-component regulatory system, designated the racr-racs (reduced ability to colonize) system, that is involved in a temperature-dependent signalling pathway. a mutation of the response regulator gene racr reduced the organism's ability to colonize the chicken intestinal tract and resulted in temperature-dependent changes in its protein profile and growth ...199910322038
cdc group iv c-2: a new ralstonia species close to ralstonia eutropha.cdc group iv c-2, an environmental gram-negative bacillus recently proposed for inclusion in the genus ralstonia, has been isolated in several human infections. biochemical characterization and 16s ribosomal dna (rdna) sequencing with phylogenetic analysis were used to characterize eight clinical isolates and four type strains. other typing tools, such as pulsed-field gel electrophoresis (pfge) and randomly amplified polymorphic dna (rapd) analysis, were also used. pfge typing of clinical isolat ...199910325323
rapid identification of thermotolerant campylobacter jejuni, campylobacter coli, campylobacter lari, and campylobacter upsaliensis from various geographic locations by a gtpase-based pcr-reverse hybridization assay.recently, a gene from campylobacter jejuni encoding a putative gtpase was identified. based on two semiconserved gtp-binding sites encoded within this gene, pcr primers were selected that allow amplification of a 153-bp fragment from c. jejuni, c. coli, c. lari, and c. upsaliensis. sequence analysis of these pcr products revealed consistent interspecies variation, which allowed the definition of species-specific probes for each of the four thermotolerant campylobacter species. multiple probes we ...199910325325
distribution of a nocardia brasiliensis catalase gene fragment in members of the genera nocardia, gordona, and rhodococcus.an immunodominant protein from nocardia brasiliensis, p61, was subjected to amino-terminal and internal sequence analysis. three sequences of 22, 17, and 38 residues, respectively, were obtained and compared with the protein database from genbank by using the blast system. the sequences showed homology to some eukaryotic catalases and to a bromoperoxidase-catalase from streptomyces violaceus. its identity as a catalase was confirmed by analysis of its enzymatic activity on h2o2 and by a double-s ...199910325357
differentiation of helicobacter pylori isolates based on lectin binding of cell extracts in an agglutination assay.plant and animal lectins with various carbohydrate specificities were used to type 35 irish clinical isolates of helicobacter pylori and the type strain nctc 11637 in a microtiter plate assay. initially, a panel of eight lectins with the indicated primary specificities were used: anguilla anguilla (aaa), lotus tetragonolobus (lotus a), and ulex europaeus i (uea i), specific for alpha-l-fucose; solanum tuberosum (sta) and triticum vulgaris (wga), specific for beta-n-acetylglucosamine; glycine max ...199910325361
times to detection of bacteria and yeasts in bactec 9240 blood culture bottles.a 7-day incubation protocol was instituted with the bactec 9240 system for a 1-year period to determine the times to detection of clinically relevant organisms. a total of 23,686 blood and 693 sterile body fluid cultures were received; some cultures were held longer by special request. of 1,609 likely skin contaminants, 42 were recovered on day 5, 34 on day 6, 16 on day 7, and 5 on day 8. of 2,803 usual pathogens, 34 were recovered on day 5, 24 on day 6, 15 on day 7 and 1 on day 8. twenty-one of ...199910325369
quinolone-resistant campylobacter jejuni infections in minnesota, 1992-1998. investigation team.increasing resistance to quinolones among campylobacter isolates from humans has been reported in europe and asia, but not in the united states. we evaluated resistance to quinolones among campylobacter isolates from minnesota residents during the period from 1992 through 1998.199910332013
passive immunity against human pathogens using bovine antibodies. 199910337007
biotin-streptavidin enzyme-linked immunosorbent assay for the detection of antibodies to campylobacter jejuni and c. coli in chickens.an enzyme-linked immunosorbent assay (elisa) was developed in a homologous system with bacterial ultrasonic-treated proteins as the antigen and antisera from chickens infected orally and subcutaneously with the strain campylobacter jejuni serovar 6 (cj 6). the cut-off level was determined using antisera from non-infected specific-pathogen-free chickens up to the age of 10 weeks. the suitability of the elisa system was verified using antisera taken from chickens orally infected at the age of 4 we ...199910337238
antibodies against helicobacter felis in sera of cats and dogs.serum samples from 61 dogs and 49 cats were screened for circulating antibodies against helicobacter felis by an enzyme-linked immunosorbent assay (elisa) using sonicated bacteria as an antigen. to improve the specificity of the elisa, sera were absorbed with campylobacter jejuni subsp. jejuni h. pylori as well as h. felis. sera from 26 dogs (43%) and 19 cats (39%) revealed clear positive absorbance readings determined as an optical density (od) that was statistically significant above the od me ...199910337240
subclass distribution and the secretory component of serum iga anti-ganglioside antibodies in guillain-barré syndrome after campylobacter jejuni enteritis.previously, we reported that iga anti-gm1 antibody is more closely associated with preceding campylobacter jejuni enteritis in guillain-barré syndrome (gbs) than are igg and igm antibodies. however, the mechanism of the induction of iga anti-ganglioside antibodies is not clear. in this study, serum iga antibodies against gm1, gm1b, and gd1a, and galnac-gd1a were examined in 152 gbs patients. in gbs, antecedent c. jejuni infection is closely associated with iga antibodies, other than gm1, against ...199910337924
serum antibody level against groel type heat-shock protein of campylobacter jejuni in patients with guillain-barré syndrome.recently there has been an increase in the number of cases reported of guillain-barré syndrome (gbs) developed after campylobacter jejuni infection. to investigate the role of a c.jejuni groel-type heat-shock protein (cjhsp60) in the infection and induction of gbs, we examined the antibody level against cjhsp60 in 27 human sera, including gbs and non-gbs patients, by an enzyme-linked immunosorbent assay. sera from patients with c. jejuni infection, despite the development of gbs, had a higher ti ...199910338193
genetic characterization of wild-type and mutant fur genes of bordetella avium.for most, if not all, organisms, iron (fe) is an essential element. in response to the nutritional requirement for fe, bacteria evolved complex systems to acquire the element from the environment. the genes encoding these systems are often coordinately regulated in response to the fe concentration. recent investigations revealed that bordetella avium, a respiratory pathogen of birds, expressed a number of fe-regulated genes (t. d. connell, a. dickenson, a. j. martone, k. t. militello, m. j. fili ...199910338537
high-resolution genotyping of campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting.for epidemiological studies of campylobacter infections, molecular typing methods that can differentiate campylobacters at the strain level are needed. in this study we used a recently developed genotyping method, amplified fragment length polymorphism (aflp), which is based on selective amplification of restriction fragments of chromosomal dna, for genetic typing of campylobacter jejuni and campylobacter coli strains derived from humans and poultry. we developed an automated aflp fingerprinting ...199910347015
generation of a superoxide dismutase (sod)-deficient mutant of campylobacter coli: evidence for the significance of sod in campylobacter survival and colonization.the microaerophilic nature of campylobacter species implies an inherent sensitivity towards oxygen and its reduction products, particularly the superoxide anion. the deleterious effects of exposure to superoxide radicals are counteracted by the activity of superoxide dismutase (sod). we have shown previously that campylobacter coli possesses an iron cofactored sod. the sodb gene of c. coli ua585 was insertionally inactivated by the site-specific insertion of a teto cassette. organisms harboring ...199910347040
response of pathogenic vibrio species to high hydrostatic pressure.vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance.199910347079
activity of gatifloxacin compared to those of five other quinolones versus aerobic and anaerobic isolates from skin and soft tissue samples of human and animal bite wound infections.the activity of gatifloxacin against 308 aerobes and 112 anaerobes isolated from bite wound infections was studied. gatifloxacin was active at </=0.016 microg/ml against all 148 pasteurella isolates (eight species and three subspecies) tested and all other aerobes tested, including actinobacillus-haemophilus spp., eikenella corrodens, neisseria weaveri, weeksella zoohelcum, staphylococci, and streptococci. fusobacteria were sometimes resistant. gatifloxacin mics at which 90% of the isolates were ...199910348774
function of proline residues of mota in torque generation by the flagellar motor of escherichia coli.bacterial flagellar motors obtain energy for rotation from the membrane gradient of protons or, in some species, sodium ions. the molecular mechanism of flagellar rotation is not understood. mota and motb are integral membrane proteins that function in proton conduction and are believed to form the stator of the motor. previous mutational studies identified two conserved proline residues in mota (pro 173 and pro 222 in the protein from escherichia coli) and a conserved aspartic acid residue in m ...199910348868
isolation and purification of a campylobacter upsaliensis autolysin.autolytic activity in the soluble and sediment fractions of sonicates of the spiral and the coccoid form of campylobacter upsaliensis could not be demonstrated by native (nondenaturing) polyacrylamide gel electrophoresis (page). autolysins were detected, however, by using denaturing sodium dodecyl sulfate (sds)-page gels containing either purified escherichia coli peptidoglycan or whole cells of micrococcus luteus (micrococcus lysodeikticus) as the turbid substrate, with subsequent renaturation ...199910349717
impact of simple screening criteria on utilization of low-yield bacterial stool cultures in a children's hospital.to determine diagnostic yield of stool cultures for salmonella, shigella, campylobacter jejuni, yersinia enterocolitica, and escherichia coli o157:h7 (sscye) among hospitalized children and to develop guidelines for appropriate use of these tests. setting. tertiary care pediatric hospital.199910353927
comparative antimicrobial activity of gatifloxacin tested against campylobacter jejuni including fluoroquinolone-resistant clinical isolates.campylobacter jejuni is an important pathogen that causes gastroenteritis, as well as other disease states such as meningitis and septic arthritis. in this study, the etest (ab biodisk, solna, sweden) results were compared to a reference agar dilution method using gatifloxacin, a new 8-methoxyfluoroquinolone. a total of 53 strains of c. jejuni initially isolated from patients in california and mexico were tested. results demonstrated a high correlation (r = 0.88) between the two utilized in vitr ...199910354858
significance of cryptosporidium in acute diarrhoea in north-eastern india.in a hospital-based study, stool samples from 2095 patients of all ages were examined for different fungal, protozoal and bacterial enteropathogens over a period of 2 years (july 1994-june 1996). cryptosporidium was detected in 151 specimens (7.2%) and was the third commonest pathogen found. the highest prevalence of this organism was in the group aged 16-45 years and during the rainy months (july-oct.). diarrhoea caused by the protozoon was of mild to moderate severity and features of dysentery ...199910359300
fatal campylobacter jejuni bacteraemia in patients with aids.two fatal cases of campylobacter jejuni septicaemia in patients with aids were characterised by severe hiv-related immunodeficiency, negative stool cultures and presentation during hospitalisation, developing a clinical picture of fulminant septic shock despite therapy with appropriate antibiotics. campylobacter spp. are important opportunist pathogens in hiv disease and may cause a septicaemic illness in the absence of enteric disease.199910359311
contamination of meat with campylobacter jejuni in saitama, japan.to determine the source of food contamination with campylobacter jejuni, we investigated retail meat, a chicken processing plant and a broiler farm. c. jejuni was found in domestic retailed poultry (45.8%) and imported poultry (3.7%), but not in beef or pork. in the poultry processing plant, there is significant contamination with c. jejuni in chicken carcasses, equipment and workers' hands. this contamination increases during the defeathering and evisceration processes. rapd analysis shows that ...199910359491
anti-galnac-gd1a antibody-associated guillain-barré syndrome with a predominantly distal weakness without cranial nerve impairment and sensory disturbance.the serum antibodies to n-acetylgalactosaminyl gd1a (galnac-gd1a) and other gangliosides as well as to campylobacter jejuni were determined in 147 patients with guillain-barré syndrome (gbs). we found a distinctive clinical pattern in patients with anti-galnac-gd1a antibodies compared with those without the antibodies, that is, lack of cranial nerve involvement (87% versus 38%), distal-dominant weakness (80% versus 25%), and no sensory disturbance (73% versus 22%). the frequency of distal-domina ...199910360768
bacterial secreted proteins are required for the internaliztion of campylobacter jejuni into cultured mammalian cells.presented here is the first evidence that campylobacter jejuni secrete proteins upon co-cultivation with host cells and in int 407 cell-conditioned medium. a c. jejuni gene designated ciab for campylobacter invasion antigen b was identified, using a differential screening technique, which is required for this secretion process and the efficient entry of this bacterium into a host cell. the c. jejuni ciab gene encodes a protein of 610 amino acids with a calculated molecular mass of 73 154 da. the ...199910361274
evidence for a system of general protein glycosylation in campylobacter jejuni.a genetic locus from campylobacter jejuni 81-176 (o:23, 36) has been characterized that appears to be involved in glycosylation of multiple proteins, including flagellin. the lipopolysaccharide (lps) core of escherichia coli dh5alpha containing some of these genes is modified such that it becomes immunoreactive with o:23 and o:36 antisera and loses reactivity with the lectin wheat germ agglutinin (wga). site-specific mutation of one of these genes in the e. coli host causes loss of o:23 and o:36 ...199910361304
molecular subtyping of clostridium perfringens by pulsed-field gel electrophoresis to facilitate food-borne-disease outbreak investigations.clostridium perfringens is a common cause of food-borne illness. the illness is characterized by profuse diarrhea and acute abdominal pain. since the illness is usually self-limiting, many cases are undiagnosed and/or not reported. investigations are often pursued after an outbreak involving large numbers of people in institutions, at restaurants, or at catered meals. serotyping has been used in the past to assist epidemiologic investigations of c. perfringens outbreaks. however, serotyping reag ...199910364587
evaluation of the anaeropack campylo system for growth of microaerophilic bacteria.growth of microaerophilic bacteria in the anaeropack campylo (mitsubishi gas chemical america, inc., new york, n.y.) atmosphere generation system was compared to growth in the campypak plus jar and campypak pouch (becton-dickinson microbiology systems [bdms], cockeysville, md.). growth in the anaeropack campylo system was considered equivalent to or better than growth obtained in the campypak plus and campypak pouch systems for 48 of the 50 helicobacter pylori strains and for all 28 campylobacte ...199910364622
bacterioferritin a modulates catalase a (kata) activity and resistance to hydrogen peroxide in pseudomonas aeruginosa.we have cloned a 3.6-kb genomic dna fragment from pseudomonas aeruginosa harboring the rpoa, rplq, kata, and bfra genes. these loci are predicted to encode, respectively, (i) the alpha subunit of rna polymerase; (ii) the l17 ribosomal protein; (iii) the major catalase, kata; and (iv) one of two iron storage proteins called bacterioferritin a (bfra; cytochrome b1 or b557). our goal was to determine the contributions of kata and bfra to the resistance of p. aeruginosa to hydrogen peroxide (h2o2). ...199910368148
fused and overlapping rpob and rpoc genes in helicobacters, campylobacters, and related bacteria.the genes coding for the beta (rpob) and beta' (rpoc) subunits of rna polymerase are fused in the gastric pathogen helicobacter pylori but separate in other taxonomic groups. to better understand how the unique fused structure evolved, we determined dna sequences at and around the rpob-rpoc junction in 10 gastric and nongastric species of helicobacter and in members of the related genera wolinella, arcobacter, sulfurospirillum, and campylobacter. we found the fusion to be specific to helicobacte ...199910368167
climate change and human health in europe. 199910373176
food production and food safety. 199910373179
human monoclonal immunoglobulin m antibodies to ganglioside gm1 show diverse cross-reactivities with lipopolysaccharides of campylobacter jejuni strains associated with guillain-barré syndrome.we examined the reactivity of a panel of anti-gm1 immunoglobulin m monoclonal antibodies (mabs) cloned from multifocal motor neuropathy patients with lipopolysaccharides (lpss) of campylobacter jejuni strains, including serotype o:41 strains associated with guillain-barré syndrome. the mabs reacted with ganglioside gm1 to different degrees, and these differences in fine specificities for gm1 were reflected in the different degrees of reactivity with each of the c. jejuni lpss tested. antibodies ...199910377164
[campylobacter jejuni enteritis and guillain-barré syndrome].guillain-barré syndrome (gbs) is the most common cause of acute neuromuscular paralysis. sera from patients with gbs following campylobacter jejuni infection frequently have autoantibody to gm 1 ganglioside in the acute phase of the illness. we revealed that the lipopolysaccharide (lps) of c. jejuni that was isolated from a gbs patient has the oligosaccharide structure [gal beta 1-3 galnac beta 1-4 (neuac alpha 2-3) gal beta 1-], which is identical to the terminal tetrasaccharide of gm 1 ganglio ...199910377788
in-vitro activity of moxifloxacin against fluoroquinolone-resistant strains of aerobic gram-negative bacilli and enterococcus faecalis.mics of the new fluoroquinolone, moxifloxacin, and those of ciprofloxacin, ofloxacin and sparfloxacin for 19 genetically characterized fluoroquinolone-resistant strains were determined by the agar dilution method. the mics of moxifloxacin for escherichia isolates with one mutation in gyra (corresponding to ser83-->leu or asp87-->gly substitution) were 0.25-0.5 mg/l, while those of ciprofloxacin, ofloxacin and sparfloxacin were 0.06-0.25, 1 and 0.12-0.5 mg/l, respectively. these values were four- ...199910382871
identification and characterization of a flagellin gene from the endosymbiont of the hydrothermal vent tubeworm riftia pachyptila.the bacterial endosymbionts of the hydrothermal vent tubeworm riftia pachyptila play a key role in providing their host with fixed carbon. results of prior research suggest that the symbionts are selected from an environmental bacterial population, although a free-living form has been neither cultured from nor identified in the hydrothermal vent environment. to begin to assess the free-living potential of the symbiont, we cloned and characterized a flagellin gene from a symbiont fosmid library. ...199910388713
viability and virulence of experimentally stressed nonculturable salmonella typhimurium.maintenance of pathogenicity of viable but nonculturable salmonella typhimurium cells experimentally stressed with uv-c and seawater, was investigated relative to the viability level of the cellular population. pathogenicity, tested in a mouse model, was lost concomitantly with culturability, whereas cell viability remained undamaged, as determined by respiratory activity and cytoplasmic membrane and genomic integrities.199910388726
rapid detection, by pcr and reverse hybridization, of mutations in the helicobacter pylori 23s rrna gene, associated with macrolide resistance.a pcr-based reverse hybridization system (research prototype kit inno-lipa for h. pylori resistance) was developed and evaluated for simultaneous detection of 23s ribosomal dna point mutations, associated with macrolide resistance in helicobacter pylori. fifty-seven h. pylori strains (51 natural, 6 laboratory-derived artificial, 52 resistant, and 5 susceptible strains) were tested by pcr-lipa (detecting mutations a2115-->g, g2141-->a, a2142-->g, a2142-->c, a2143-->g, a2143-->c, and a2143-->t), d ...199910390244
severe guillain-barré syndrome associated with campylobacter jejuni infection with failure to respond to plasmapheresis and immunoglobulin. 199910395423
inhibition of salmonella typhimurium in the chicken intestinal tract by a transformed avirulent avian escherichia coli.an avirulent, wild-type avian escherichia coli (e. coli av) was electrotransformed with a plasmid coding for the production of microcin 24 (pgob18) and was designated e. coli avgob18. the transformant inhibited the growth of seven serotypes of salmonella commonly associated with colonization and contamination of poultry products and seven strains of e. coli o157:h7 in the in vitro colicin/microcin assay. the transformant did not inhibit the replication of multiple isolates of listeria monocytoge ...199910396637
the chemotactic response of vibrio anguillarum to fish intestinal mucus is mediated by a combination of multiple mucus components.chemotactic motility has previously been shown to be essential for the virulence of vibrio anguillarum in waterborne infections of fish. to investigate the mechanisms by which chemotaxis may function during infection, mucus was isolated from the intestinal and skin epithelial surfaces of rainbow trout. chemotaxis assays revealed that v. anguillarum swims towards both types of mucus, with a higher chemotactic response being observed for intestinal mucus. work was performed to examine the basis, i ...199910400589
the genomic tree as revealed from whole proteome comparisons.the availability of a number of complete cellular genome sequences allows the development of organisms' classification, taking into account their genome content, the loss or acquisition of genes, and overall gene similarities as signatures of common ancestry. on the basis of correspondence analysis and hierarchical classification methods, a methodological framework is introduced here for the classification of the available 20 completely sequenced genomes and partial information for schizosacchar ...199910400922
autoantibodies to gm1b and galnac-gd1a: relationship to campylobacter jejuni infection and acute motor axonal neuropathy in china.igg antibodies to the minor gangliosides gm1b and galnac-gd1a frequently are present in sera of japanese patients with guillain-barré syndrome. the relationship between these autoantibodies and campylobacter jejuni infection, the type of disease (acute motor axonal neuropathy [aman], or acute inflammatory demyelinating polyneuropathy [aidp]) has yet to be established. sera samples were obtained from 55 chinese patients with clinically defined guillain-barré syndrome. an electrophysiology study s ...199910402024
no benefit of long-term ciprofloxacin treatment in patients with reactive arthritis and undifferentiated oligoarthritis: a three-month, multicenter, double-blind, randomized, placebo-controlled study.to investigate the effect of long-term antibiotic treatment in patients with reactive arthritis (rea) and undifferentiated oligoarthritis.199910403266
comparison of a commercial test for serotyping heat-stable antigens of campylobacter jejuni with genotyping by pulsed-field gel electrophoresis.a new commercial serotyping set based on heat-stable penner's antigens was compared with pulsed-field gel electrophoresis (pfge) with smai and sacii restriction endonucleases. among 50 isolates of campylobacter jejuni from finnish patients, which represented predominant pfge patterns selected from isolates from sporadic cases and isolates associated with small outbreaks, 11 different serotypes were demonstrated from 43 typable isolates. several pfge patterns could be found within one serotype; o ...199910403411
comparison of molecular methods for typing vibrio parahaemolyticus.an outbreak of vibrio parahaemolyticus gastroenteritis on canada's west coast in 1997 emphasized the need to develop molecular methods for differentiation and typing of these organisms. isolates were analyzed by enterobacterial repetitive intergenic consensus sequence (eric) pcr, detection of restriction fragment length polymorphisms (rflp) in rrna genes (ribotyping), pulsed-field gel electrophoresis (pfge), and rflp analysis of the genetic locus encoding the polar flagellum (fla locus rflp anal ...199910405387
value of enterobacterial repetitive intergenic consensus pcr for study of pasteurella multocida strains isolated from mouths of dogs.fifty-six pasteurella multocida strains (40 p. multocida subsp. septica and 16 p. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a french canine military training center (132e groupe cynophile de l'armée de terre, suippes, france) were studied by use of enterobacterial repetitive intergenic consensus-pcr (eric-pcr) and restriction fragment length polymorphism (rflp) techniques. both techniques showed genomic heterogeneity of the strains studied. h ...199910405390
disseminated zygomycosis due to rhizopus schipperae after heatstroke.a 21-year-old woman suffered heatstroke and developed diarrhea while trekking across south texas. the heatstroke was complicated by seizures, rhabdomyolysis, pneumonia, renal failure, and disseminated intravascular coagulation. the patient's stool and blood cultures grew campylobacter jejuni. the patient subsequently developed paranasal and gastrointestinal zygomycosis and required surgical debridement and a prolonged course of amphotericin b. the zygomycete cultured was rhizopus schipperae. thi ...199910405417
an uncommon helicobacter isolate from blood: evidence of a group of helicobacter spp. pathogenic in aids patients.an unusual helicobacter sp. was isolated from the blood of a human immunodeficiency virus (hiv)-infected patient. this organism had spiral morphology, with single amphitrichous flagella, and was negative for hippurate hydrolysis, production of urease, and reduction of nitrate. 16s rrna gene sequence analysis verified that the isolate was a species of helicobacter, most closely related to an undescribed helicobacter-like isolate from vancouver, british columbia, canada, and to helicobacter westme ...199910405434
high-performance liquid chromatography determination of residue levels on chicken carcasses treated with cetylpyridinium chloride.cetylpyridinium chloride (cpc) has been found to be effective in reducing contamination of chicken carcasses from a variety of microorganisms, including escherichia coli o157:h7, salmonella typhimurium, campylobacter jejuni, aeromonas hydrophila, listeria monocytogenes, and staphylococcus aureus. a procedure has been developed to determine residue levels on chicken carcasses after cpc treatment. for the analysis, chicken carcasses were extracted with 95% ethanol. the cpc concentration in the ext ...199910406212
hyperreflexia in guillain-barré syndrome: relation with acute motor axonal neuropathy and anti-gm1 antibody.to investigate the incidence of hyperreflexia in patients with guillain-barré syndrome (gbs), and its relation with electrodiagnosis of acute motor axonal neuropathy (aman), antiganglioside gm1 antibody, and campylobacter jejuni infection. it was reported that patients with aman in northern china often had hyperreflexia in the recovery phase.199910406985
host-pathogen interactions: redefining the basic concepts of virulence and pathogenicity. 199910417127
characterization of a haemophilus ducreyi mutant deficient in expression of cytolethal distending toxin.haemophilus ducreyi expresses a soluble cytolethal distending toxin (cdt) that kills hela, hep-2, and other human epithelial cells in vitro. h. ducreyi cdt activity is encoded by a three-gene cluster (cdtabc), and antibody to the cdtc gene product can neutralize cdt activity in vitro (l. d. cope, s. r. lumbley, j. l. latimer, j. klesney-tait, m. k. stevens, l. s. johnson, m. purven, r. s. munson, jr., t. lagergard, j. d. radolf, and e. j. hansen, proc. natl. acad. sci. usa 94:4056-4061, 1997). c ...199910417154
campylobacter jejuni 81-176 associates with microtubules and dynein during invasion of human intestinal cells.campylobacter jejuni uptake into cultured int407 cells was analyzed kinetically over a wide range of starting multiplicities of infection (moi; from 0.02 to 20,000 bacteria/epithelial cell). the efficiency of internalization was the highest at moi of 0.02 and decreased steadily at higher mois, presumably due to reported c. jejuni autoagglutination at higher densities. total internalized campylobacter cfu increased gradually from an moi of 0.02 to a peak at an moi of 200 (reaching an average of t ...199910417189
citrobacter freundii invades and replicates in human brain microvascular endothelial cells.neonatal bacterial meningitis remains a disease with unacceptable rates of morbidity and mortality despite the availability of effective antimicrobial therapy. citrobacter spp. cause neonatal meningitis but are unique in their frequent association with brain abscess formation. the pathogenesis of citrobacter spp. causing meningitis and brain abscess is not well characterized; however, as with other meningitis-causing bacteria (e.g., escherichia coli k1 and group b streptococci), penetration of t ...199910417193
helicobacter pylori induces gastric epithelial cell apoptosis in association with increased fas receptor expression.the mechanisms involved in mediating the enhanced gastric epithelial cell apoptosis observed during infection with helicobacter pylori in vivo are unknown. to determine whether h. pylori directly induces apoptosis of gastric epithelial cells in vitro and to define the role of the fas-fas ligand signal transduction cascade, human gastric epithelial cells were infected with h. pylori for up to 72 h under microaerophilic conditions. as assessed by both transmission electron microscopy and fluoresce ...199910417197
antibiotic resistance: a current perspective. 199910417485
occurrence, removal and seasonal variation of thermophilic campylobacters and arcobacter in sewage sludge.the presence of thermophilic campylobacters and arcobacter was investigated in four types of sewage sludge taken from the treatment plant in bologna (italy): primary, activated, thickened and anaerobically digested sludge. campylobacter jejuni and campylobacter coli were more numerous during the march-september period and were found only in primary sludge (22.7%) with mean counts of 278 mpn/g dry matter and 1403 mpn/g dry matter respectively. arcobacter butzleri were found in all types of sludge ...199910418097
interferon-alpha in viral and bacterial gastroenteritis: a comparison with c-reactive protein and interleukin-6.the aim of the study was to identify serum markers able to differentiate bacterial and viral origin in acute diarrhoea. interferon-alpha (inf-alpha), c-reactive protein (crp) and interleukin-6 were determined on admission in the sera of 119 children aged between 1 mo and 14 y who were hospitalized for rotavirus (n = 60) or bacterial diarrhoea (salmonella spp. 39 cases, shigella spp. 15 cases, campylobacter jejuni 5 cases). crp concentration was >10 mg/l in 48.3% of children with viral gastroente ...199910419239
incidence of salmonella, campylobacter jejuni, campylobacter coli, and listeria monocytogenes in poultry carcasses and different types of poultry products for sale on the belgian retail market.from january 1997 to may 1998, 772 samples of poultry carcasses and poultry products for sale on the retail market in belgium were analyzed for the presence of salmonella spp., salmonella enteritidis, campylobacter jejuni, c. coli, and listeria monocytogenes per 100 cm2 or 25 g. poultry samples were contaminated with salmonella (36.5%), c. jejuni and c. coli (28.5%), and l. monocytogenes (38.2%). in about 12.3% of the poultry samples, the l. monocytogenes contamination level exceeded 1 cfu per g ...199910419264
characterization of an atypical superoxide dismutase from sinorhizobium meliloti.sinorhizobium meliloti rm5000 is an aerobic bacterium that can live free in the soil or in symbiosis with the roots of leguminous plants. a single detectable superoxide dismutase (sod) was found in free-living growth conditions. the corresponding gene was isolated from a genomic library by using a sod fragment amplified by pcr from degenerate primers as a probe. the soda gene was located in the chromosome. it is transcribed monocistronically and encodes a 200-amino-acid protein with a theoretica ...199910419947
[myopericarditis caused by campylobacter jejuni]. 199910420889
identification of the yqhe and yafb genes encoding two 2, 5-diketo-d-gluconate reductases in escherichia coli.the identification of a gene (yiae) encoding 2-ketoaldonate reductase (2kr) in our previous work led to the hypothesis that escherichia coli has other ketogluconate reductases including 2, 5-diketo-d-gluconate reductase (25dkgr) and to study of the related ketogluconate metabolism. by using the deduced amino acid sequences of 5-diketo-d-gluconate reductase (5kdgr) of gluconobacter oxydans and 25dkgr of corynebacterium sp., protein databases were screened to detect homologous proteins. among the ...199910427017
microbial population changes during bioremediation of an experimental oil spill.three crude oil bioremediation techniques were applied in a randomized block field experiment simulating a coastal oil spill. four treatments (no oil control, oil alone, oil plus nutrients, and oil plus nutrients plus an indigenous inoculum) were applied. in situ microbial community structures were monitored by phospholipid fatty acid (plfa) analysis and 16s rdna pcr-denaturing gradient gel electrophoresis (dgge) to (i) identify the bacterial community members responsible for the decontamination ...199910427050
restriction fragment length polymorphism analysis of the flaa gene of campylobacter jejuni for subtyping human, animal and poultry isolates.233 strains of campylobacter jejuni were subtyped by pcr-rflp analysis of the flagellin (flaa) gene by double digestion with ecori and psti (ep flaa-profiling). the strains represented a variety of common penner heat stable (hs) serotypes and comprised isolates of human, bovine, ovine, chicken and canine origin. flaa amplicons were obtained directly from dna in cell lysates of most strains. rflp analysis showed considerable allelic variation and nine ep flaa-types were identified of which the mo ...199910427717
a highly conserved sequence is a novel gene involved in de novo vitamin b6 biosynthesis.the cercospora nicotianae sor1 (singlet oxygen resistance) gene was identified previously as a gene involved in resistance of this fungus to singlet-oxygen-generating phototoxins. although homologues to sor1 occur in organisms in four kingdoms and encode one of the most highly conserved proteins yet identified, the precise function of this protein has, until now, remained unknown. we show that sor1 is essential in pyridoxine (vitamin b6) synthesis in c. nicotianae and aspergillus flavus, althoug ...199910430950
differential susceptibility to hepatic inflammation and proliferation in axb recombinant inbred mice chronically infected with helicobacter hepaticus.helicobacter hepaticus is a naturally occurring pathogen of mice and has been used to develop models of chronic hepatitis, liver cancer, and, more recently, inflammatory bowel disease, in selected mouse strains. a/jcr mice are particularly susceptible to h. hepaticus-induced hepatitis and subsequent development of liver neoplasms, whereas c57bl/6 mice are resistant. in this study, we inoculated nine axb recombinant inbred (ri) mouse strains, derived from a/j and c57bl/6 mice, with h. hepaticus t ...199910433949
an iron-regulated alkyl hydroperoxide reductase (ahpc) confers aerotolerance and oxidative stress resistance to the microaerophilic pathogen campylobacter jejuni.microaerophiles like campylobacter jejuni must resist oxidative stresses during transmission or infection. growth of c. jejuni 81116 under iron limitation greatly increased the expression of two polypeptides of 26 and 55 kda. the identification of these proteins by n-terminal amino acid sequencing showed both to be involved in the defense against oxidative stress. the 55-kda polypeptide was identical to c. jejuni catalase (kata), whereas the n terminus of the 26-kda polypeptide was homologous to ...199910438747
evaluation of cytokine production and phagocytic activity in mice infected with campylobacter jejuni.the effect of several campylobacter jejuni strains on the immune response was analyzed in mice after intraperitoneal inoculation with 10(10) colony forming units (cfu). three c. jejuni strains were assayed: ccug 6968 (enterotoxigenic), ccug 7580 (enterotoxigenic), and ccug 7440 (non-enterotoxigenic). these c. jejuni strains induced a peritoneal inflammatory response and an important increase in the peritoneal phagocyte oxidative activity measured by chemiluminescence assay, as well as an increas ...199910441725
retail poultry and beef as sources of campylobacter jejuni.to investigate the level of contamination with c. jejuni of raw chicken and beef meats sold in nairobi and to assess their potential as sources of campylobacter infections to man.199910442113
campylobacter jejuni and hemolytic-uremic syndrome. 199910450044
food-borne antibiotic-resistant campylobacter infections.a recent report has provided some important information concerning the apparent emergence of quinolone-resistant bacterial infections in the united states. in particular, this report indicates an increase in domestically acquired, quinolone-resistant campylobacter jejuni infections, likely associated with poultry consumption.199910453177
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