Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| nutrients, microglia aging, and brain aging. | as the life expectancy continues to increase, the cognitive decline associated with alzheimer's disease (ad) becomes a big major issue in the world. after cellular activation upon systemic inflammation, microglia, the resident immune cells in the brain, start to release proinflammatory mediators to trigger neuroinflammation. we have found that chronic systemic inflammatory challenges induce differential age-dependent microglial responses, which are in line with the impairment of learning and mem ... | 2016 | 26941889 |
| genome-wide association study of biologically informed periodontal complex traits offers novel insights into the genetic basis of periodontal disease. | genome-wide association studies (gwas) of chronic periodontitis (cp) defined by clinical criteria alone have had modest success to-date. here, we refine the cp phenotype by supplementing clinical data with biological intermediates of microbial burden (levels of eight periodontal pathogens) and local inflammatory response (gingival crevicular fluid il-1β) and derive periodontal complex traits (pcts) via principal component analysis. pcts were carried forward to gwas (∼2.5 million markers) to iden ... | 2016 | 26962152 |
| management of diabolical diabetes mellitus and periodontitis nexus: are we doing enough? | periodontitis is the commonest oral disease affecting population worldwide. this disease is notorious for the devastation of tooth supporting structures, ensuing in the loss of dentition. the etiology for this disease is bacterial biofilm, which accumulates on the teeth as dental plaque. in addition to the biofilm microorganisms, other factors such as environmental, systemic and genetic are also responsible in progression of periodontitis. diabetes mellitus (dm) is metabolic disorder which has a ... | 2016 | 26962409 |
| hand to mouth: a systematic review and meta-analysis of the association between rheumatoid arthritis and periodontitis. | rheumatoid arthritis (ra) and periodontitis are both chronic inflammatory diseases, which demonstrate similarities in terms of mechanism, histopathology, and demography. an association between these conditions has been demonstrated previously but has been called into question more recently. | 2016 | 26973655 |
| microorganisms in periradicular tissues: do they exist? a perennial controversy. | there is no greater association between the basic science and the practice of endodontics than that of microbiology. one of the strongest factors contributing to the controversies often encountered in the endodontic field is the lack of understanding that the disease processes of the pulp and periradicular tissues generally have a microbiological etiology. the vast majority of diseases of dental pulp and periradicular tissues are associated with microorganisms. after the microbial invasion of th ... | 2015 | 26980965 |
| oral microbiota species in acute apical endodontic abscesses. | acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. | 2016 | 26983837 |
| microrna expression profile of human periodontal ligament cells under the influence of porphyromonas gingivalis lps. | periodontitis is a chronic inflammatory disease which is caused by bacterial infection and leads to the destruction of periodontal tissues and resorption of alveolar bone. thus, special attention should be paid to the mechanism under lipopolysaccharide (lps)-induced periodontitis because lps is the major cause of periodontitis. however, to date, mirna expression in the lps-induced periodontitis has not been well characterized. in this study, we investigated mirna expression patterns in lps-treat ... | 2016 | 26987780 |
| influence of type 2 diabetes on prevalence of key periodontal pathogens, salivary matrix metalloproteinases, and bone remodeling markers in sudanese adults with and without chronic periodontitis. | this study compared the influence of type 2 diabetes on the occurrence of six periodontal pathogens in plaque samples of patients with and without chronic periodontitis. levels of salivary mmp-8, mmp-9, rankl, and opg were also investigated. the study enrolled 31 patients with type 2 diabetes and chronic periodontitis (dm + cp), 29 with chronic periodontitis (cp), and 20 with type 2 diabetes (dm). questionnaire-guided interviews were conducted and plaque index, bleeding on probing, and pocket de ... | 2016 | 26989414 |
| arthritis-induced alveolar bone loss is associated with changes in the composition of oral microbiota. | rheumatoid arthritis (ra) and periodontitis (pd) are chronic inflammatory disorders that cause bone loss. pd tends to be more prevalent and severe in ra patients. previous experimental studies demonstrated that ra triggers alveolar bone loss similarly to pd. the aim of this study was to investigate if arthritis-induced alveolar bone loss is associated with modification in the oral microbiota. checkerboard dna-dna hybridization was employed to analyze forty oral bacterial species in 3 groups of c ... | 2016 | 26996070 |
| systematic engineering of the central metabolism in escherichia coli for effective production of n-butanol. | microbes have been extensively explored for production of environment-friendly fuels and chemicals. the microbial fermentation pathways leading to these commodities usually involve many redox reactions. this makes the fermentative production of highly reduced products challenging, because there is a limited nadh output from glucose catabolism. microbial production of n-butanol apparently represents one typical example. | 2016 | 26997975 |
| the outer-membrane export signal of porphyromonas gingivalis type ix secretion system (t9ss) is a conserved c-terminal β-sandwich domain. | in the recently characterized type ix secretion system (t9ss), the conserved c-terminal domain (ctd) in secreted proteins functions as an outer membrane translocation signal for export of virulence factors to the cell surface in the gram-negative bacteroidetes phylum. in the periodontal pathogen porphyromonas gingivalis, the ctd is cleaved off by poru sortase in a sequence-independent manner, and anionic lipopolysaccharide (a-lps) is attached to many translocated proteins, thus anchoring them to ... | 2016 | 27005013 |
| c/ebp β mediates endoplasmic reticulum stress regulated inflammatory response and extracellular matrix degradation in lps-stimulated human periodontal ligament cells. | periodontitis is an oral inflammatory disease that not only affects the integrity of local tooth-supporting tissues but also impacts systemic health. a compositional shift in oral microbiota has been considered as the main cause of periodontitis; however, the potential mechanism has not been fully defined. herein, we investigated the role of ccaat/enhancer-binding protein β (c/ebp β), a member of the c/ebp family of transcription factors, in human periodontal ligament cells (hpdlcs) exposed to p ... | 2016 | 27011164 |
| oxidative stress and periodontal disease in obesity. | periodontal disease is a chronic inflammatory disease of the jaws and is more prevalent in obesity. local and systemic oxidative stress may be an early link between periodontal disease and obesity. the primary aim of this study was to detect whether increased periodontal disease susceptibility in obese individuals is associated with local and systemic oxidative stress. accordingly; we analyzed periodontal status and systemic (serum) and local (gingival crevicular fluid [gcf]) oxidative status ma ... | 2016 | 27015191 |
| neutrophil functions in periodontal homeostasis. | oral tissues are constantly exposed to damage from the mechanical effort of eating and to microorganisms, mostly bacteria. in healthy gingiva tissue remodeling and a balance between bacteria and innate immune cells are maintained. however, excess of bacteria biofilm (plaque) creates an inflammation state that recruits more immune cells, mainly neutrophils to the gingiva. neutrophils create a barrier for bacteria to reach inside tissues. when neutrophils are insufficient, bacteria thrive causing ... | 2016 | 27019855 |
| accuracy of commercial kits and published primer pairs for the detection of periodontopathogens. | despite the input of microbiome research, a group of 20 bacteria continues to be the focus of periodontal diagnostics and therapy. the aim of this study was to compare three commercial kits and laboratory-developed primer pairs for effectiveness in detecting such periodontopathogens. | 2016 | 27020914 |
| [the molecular genetic characteristic of species content of saliva and gingival recess under periodontitis]. | the examination was carried out of samplings of 110 patients with periodontitis (observation group) and 60 patients without pathology of periodont (comparison group). the polymerase chain reaction was used to analyze samples of saliva and contents of periodontal recesses for detecting species-specific dna fragments of porphymmonas gigngivalis, streptococcus macacae, s. mutans, s. oralis, s. salivarius, s. sangis, s. sobrinus, treponema denticola. in patients with periodontitis s. mutans, s. oral ... | 2015 | 27032255 |
| differential responses of pattern recognition receptors to outer membrane vesicles of three periodontal pathogens. | highly purified outer membrane vesicles (omvs) of the periodontal pathogens, porphyromonas gingivalis, treponema denticola and tannerella forsythia were produced using tangential flow ultrafiltration, ultracentrifugation and optiprep density gradient separation. cryo-tem and light scattering showed omvs to be single lipid-bilayers with modal diameters of 75 to 158 nm. enumeration of omvs by nanoparticle flow-cytometry at the same stage of late exponential culture indicated that p. gingivalis was ... | 2016 | 27035339 |
| mucosal and salivary microbiota associated with recurrent aphthous stomatitis. | recurrent aphthous stomatitis (ras) is a common oral mucosal disorder of unclear etiopathogenesis. although recent studies of the oral microbiota by high-throughput sequencing of 16s rrna genes have suggested that imbalances in the oral microbiota may contribute to the etiopathogenesis of ras, no specific bacterial species associated with ras have been identified. the present study aimed to characterize the microbiota in the oral mucosa and saliva of ras patients in comparison with control subje ... | 2016 | 27036492 |
| microbial synthesis of propane by engineering valine pathway and aldehyde-deformylating oxygenase. | propane, a major component of liquid petroleum gas (lpg) derived from fossil fuels, has widespread applications in vehicles, cooking, and ambient heating. given the concerns about fossil fuel depletion and carbon emission, exploiting alternative and renewable source of propane have become attractive. in this study, we report the construction of a novel propane biosynthetic pathway in escherichia coli. | 2016 | 27042209 |
| agrobacterium tumefaciens zur regulates the high-affinity zinc uptake system trocba and the putative metal chaperone ycic, along with zint and znuabc, for survival under zinc-limiting conditions. | agrobacterium tumefaciens has a cluster of genes (atu3178, atu3179, and atu3180) encoding an abc-type transporter, here named troa, trob, and troc, respectively, which is shown here to be a zinc-specific uptake system. reverse transcription (rt)-pcr analysis confirmed that troa, trob, and troc are cotranscribed, with troc as the first gene of the operon. the ycic (atu3181) gene is transcribed in the opposite orientation to that of the trocba operon and belongs to a metal-binding gtpase family. e ... | 2016 | 27060116 |
| comparison of the efficacy of subgingival irrigation with 2% povidone-iodine and tetracycline hcl in subjects with chronic moderate periodontitis: a clinico microbiological study. | this study was performed to evaluate and compare the clinical and antimicrobial efficacy of subgingival irrigation with tetracycline and povidone-iodine as an adjunct to nonsurgical periodontal therapy. | 2016 | 27076823 |
| tongue coating and the salivary microbial communities vary in children with halitosis. | halitosis is a common symptom mainly caused by microbial activities in the oral cavity. here, we used 16s rrna gene pyrosequencing and metagenomic sequencing to examine oral microbial compositions and their functional variations in children with halitosis. we found that the tongue coating of subjects with halitosis had greater bacterial richness than those of healthy subjects. the relative abundance and prevalence of leptotrichia wadei and peptostreptococcus stomatis were higher in tongue coatin ... | 2016 | 27080513 |
| the microbiome associated with equine periodontitis and oral health. | equine periodontal disease is a common and painful condition and its severe form, periodontitis, can lead to tooth loss. its aetiopathogenesis remains poorly understood despite recent increased awareness of this disorder amongst the veterinary profession. bacteria have been found to be causative agents of the disease in other species, but current understanding of their role in equine periodontitis is extremely limited. the aim of this study was to use high-throughput sequencing to identify the m ... | 2016 | 27080859 |
| assessment of the quantity of microorganisms associated with bronchiectasis in saliva, sputum and nasal lavage after periodontal treatment: a study protocol of a randomised controlled trial. | the association between periodontal disease (pd) and chronic obstructive pulmonary disease (copd) has been widely studied, with aspiration of periodontal pathogens being one of the most accepted causal mechanisms for pulmonary exacerbation. periodontal treatment (pt) was associated with a decrease in these exacerbations. bronchiectasis is a pulmonary disease that has many similarities to copd; however, there are no studies correlating this condition to pd thus far. this study will evaluate if pt ... | 2016 | 27084279 |
| microbiota-based signature of gingivitis treatments: a randomized study. | plaque-induced gingivitis can be alleviated by various treatment regimens. to probe the impacts of various anti-gingivitis treatments on plaque microflora, here a double blinded, randomized controlled trial of 91 adults with moderate gingivitis was designed with two anti-gingivitis regimens: the brush-alone treatment and the brush-plus-rinse treatment. in the later group, more reduction in both plaque index (tmqhi) and gingival index (mean mgi) at day 3, day 11 and day 27 was evident, and more d ... | 2016 | 27094556 |
| the treponema denticola fhbb protein is a dominant early antigen that elicits fhbb variant-specific antibodies that block factor h binding and cleavage by dentilisin. | the treponema denticola fhbb protein contributes to immune evasion by binding factor h (fh). cleavage of fh by the t. denticola protease, dentilisin, may contribute to the local immune dysregulation that is characteristic of periodontal disease (pd). although three fhbb phyletic types have been defined (fhbb1, fhbb2, and fhbb3), the in vivo expression patterns and antigenic heterogeneity of fhbb have not been assessed. here, we demonstrate that fhbb is a dominant early antigen that elicits fhbb ... | 2016 | 27113359 |
| single-enzyme biomineralization of cadmium sulfide nanocrystals with controlled optical properties. | nature has evolved several unique biomineralization strategies to direct the synthesis and growth of inorganic materials. these natural systems are complex, involving the interaction of multiple biomolecules to catalyze biomineralization and template growth. herein we describe the first report to our knowledge of a single enzyme capable of both catalyzing mineralization in otherwise unreactive solution and of templating nanocrystal growth. a recombinant putative cystathionine γ-lyase (smcse) min ... | 2016 | 27118834 |
| detection of cfxa2, cfxa3, and cfxa6 genes in beta-lactamase producing oral anaerobes. | purpose the aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxa and blatem genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. material and methods periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. presumptive β-lactamase-producing isolates were evaluated for ... | 2016 | 27119762 |
| evidence of in vivo existence of borrelia biofilm in borrelial lymphocytomas. | lyme borreliosis, caused by the spirochete borrelia burgdorferi sensu lato, has grown into a major public health problem. we recently identified a novel morphological form of b. burgdorferi, called biofilm, a structure that is well known to be highly resistant to antibiotics. however, there is no evidence of the existence of borrelia biofilm in vivo; therefore, the main goal of this study was to determine the presence of borrelia biofilm in infected human skin tissues. archived skin biopsy tissu ... | 2016 | 27141311 |
| detection of five potentially periodontal pathogenic bacteria in peri-implant disease: a comparison of pcr and real-time pcr. | the aim of this study was to compare the microbial analysis methods of polymerase chain reaction (pcr) and real-time pcr (rt-pcr) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using pcr (dna-strip technology) and rt-pcr (fluorescent dye sybr green-system): aggregatibacter actinomycetemcomitans (aa ... | 2016 | 27142589 |
| the efficacy of er,cr:ysgg laser supported periodontal therapy on the reduction of peridodontal disease related oral malodor: a randomized clinical study. | this study aims to evaluate the efficacy of er,cr:ysgg laser assisted periodontal therapy on the reduction of oral malodor and periodontal disease. | 2016 | 27145828 |
| histoplasma capsulatum-induced cytokine secretion in lung epithelial cells is dependent on host integrins, src-family kinase activation, and membrane raft recruitment. | histoplasma capsulatum var. capsulatum is a dimorphic fungus that causes histoplasmosis, a human systemic mycosis with worldwide distribution. in the present work, we demonstrate that h. capsulatum yeasts are able to induce cytokine secretion by the human lung epithelial cell line a549 in integrin- and src-family kinase (sfk)-dependent manners. this conclusion is supported by small interfering rna (sirna) directed to α3 and α5 integrins, and pp2, an inhibitor of sfk activation. sirna and pp2 red ... | 2016 | 27148251 |
| efficacy of local use of probiotics as an adjunct to scaling and root planing in chronic periodontitis and halitosis: a randomized controlled trial. | periodontitis is known to have multifactorial etiology, involving interplay between environmental, host and microbial factors. the current treatment approaches are aimed at reducing the pathogenic microorganisms. administration of beneficial bacteria (probiotics) has emerged as a promising concept in the prevention and treatment of periodontitis. thus, the aim of the present study is to evaluate the efficacy of the local use of probiotics as an adjunct to scaling and root planing (srp) in the tr ... | 2016 | 27162801 |
| [use of the real-time pcr for study of the periodontal microbiome in patients with combined pathology of gastroduodenal zone and chronic periodontitis]. | the total of 54 patients with chronic periodontitis of different severity was tested using real-time pcr (dentoflor kit). the group included 38 patients with chronic gastritis. for the first time, a higher prevalence of treponema denticola in periodontium of males in comparison with females was demonstrated. the patients with chronic gastritis had more human genome dna at their periodontium than healthy individuals. non-parametric statistical analysis demonstrated high association of periodontiu ... | 2016 | 27183718 |
| multilocus sequence typing of pathogenic treponemes isolated from cloven-hoofed animals and comparison to treponemes isolated from humans. | treponema species are implicated in many diseases of humans and animals. digital dermatitis (dd) treponemes are reported to cause severe lesions in cattle, sheep, pigs, goats, and wild elk, causing substantial global animal welfare issues and economic losses. the fastidiousness of these spirochetes has previously precluded studies investigating within-phylogroup genetic diversity. an archive of treponemes that we isolated enabled multilocus sequence typing to quantify the diversity and populatio ... | 2016 | 27208135 |
| polymeric nanoparticle-based photodynamic therapy for chronic periodontitis in vivo. | antimicrobial photodynamic therapy (apdt) is increasingly being explored for treatment of periodontitis. here, we investigated the effect of apdt on human dental plaque bacteria in suspensions and biofilms in vitro using methylene blue (mb)-loaded poly(lactic-co-glycolic) (plga) nanoparticles (mb-np) and red light at 660 nm. the effect of mb-np-based apdt was also evaluated in a clinical pilot study with 10 adult human subjects with chronic periodontitis. dental plaque samples from human subject ... | 2016 | 27213356 |
| a novel species of torque teno mini virus (ttmv) in gingival tissue from chronic periodontitis patients. | a new species of torque teno mini virus, named ttmv-222, was detected in gingival tissue from periodontitis patients using a viral metagenomics method. the 2803-nucleotide genome of ttmv-222 is closely related to ttmv1-cbd279, with 62.6% overall nucleotide similarity. genetic analyses of the new virus genome revealed a classic genomic organization but a weak identity with known sequences. the prevalence of ttmv-222 in the periodontitis group (n = 150) was significantly higher than that in the he ... | 2016 | 27221159 |
| global tlr2 and 4 deficiency in mice impacts bone resorption, inflammatory markers and atherosclerosis to polymicrobial infection. | toll-like-receptors (tlrs) play a significant role in the generation of a specific innate immune response against invading pathogens. tlr2 and tlr4 signaling contributes to infection-induced inflammation in periodontal disease (pd) and atherosclerosis. observational studies point towards a relationship between pd and atherosclerosis, but the role of tlr2 and tlr4 in the recognition of multiple oral pathogens and their modulation of host response leading to atherosclerosis are not clear. we evalu ... | 2016 | 27224005 |
| effects of bacterial community members on the proteome of the ammonia-oxidizing bacterium nitrosomonas sp. strain is79. | microorganisms in the environment do not exist as the often-studied pure cultures but as members of complex microbial communities. characterizing the interactions within microbial communities is essential to understand their function in both natural and engineered environments. in this study, we investigated how the presence of a nitrite-oxidizing bacterium (nob) and heterotrophic bacteria affect the growth and proteome of the chemolithoautotrophic ammonia-oxidizing bacterium (aob) nitrosomonas ... | 2016 | 27235442 |
| periodontal disease's contribution to alzheimer's disease progression in down syndrome. | people with down syndrome (ds) are at an increased risk for alzheimer's disease (ad). after 60 years of age, >50% of ds subjects acquire dementia. nevertheless, the age of onset is highly variable possibly because of both genetic and environmental factors. genetics cannot be modified, but environmental risk factors present a potentially relevant intervention for ds persons at risk for ad. among them, inflammation, important in ad of ds type, is potential target. consistent with this hypothesis, ... | 2016 | 27239536 |
| [study of mutual dependence of periodontal and colonic microbiome in health and pathology using nsg-sequencing]. | by using ngs-sequencing libraries of dna from periodontal swabs with primers specific to v6 region of 16s rdna prevalence of bacterial genera and species in periodontal and colonic microbiota of patients with periodontitis of different severity and healthy donors was analyzed. hyper-colonization of the colon with akkermansia muciniphila was found to be the most important maker of negative predisposition to periodontitis (t=133,7 at р=10(-6)). this result is in a good agreement with communication ... | 2016 | 27239990 |
| adjunctive use of essential oils following scaling and root planing -a randomized clinical trial. | hitherto no study has been published on the effect of the adjunctive administration of essential oils following scaling and root planing (srp). this study describes the effect of a mouthrinse consisting of essential oils (cymbopogon flexuosus, thymus zygis and rosmarinus officinalis) following srp by clinical and microbiological variables in patients with generalized moderate chronic periodontitis. | 2016 | 27266517 |
| molecular-level evaluation of selected periodontal pathogens from subgingival regions in canines and humans with periodontal disease. | dogs commonly serve as a model for various human conditions, including periodontal diseases. the aim of this study was to identify the anaerobic bacteria that colonize the subgingival areas in dogs and humans by using rapid real-time polymerase chain reaction (rt-pcr)-based tests and to compare the results obtained in each species. bacterial microflora evaluations, both quantitative and qualitative, were performed by applying ready-made tests on twelve dogs and twelve humans. five samples were c ... | 2017 | 27297417 |
| putative periodontopathic bacteria and herpesviruses in pregnant women: a case-control study. | little is known about herpesvirus and putative periodontopathic bacteria in maternal chronic periodontitis. the present case-control study aimed to explore the potential relationship between putative periodontopathic bacteria and herpesviruses in maternal chronic periodontitis.saliva samples were collected from 36 pregnant women with chronic periodontitis (cases) and 36 pregnant women with healthy periodontal status (controls). six putative periodontopathic bacteria (porphyromonas gingivalis [pg ... | 2016 | 27301874 |
| detection of novel integrons in the metagenome of human saliva. | integrons are genetic elements capable of capturing and expressing open reading frames (orfs) embedded within gene cassettes. they are involved in the dissemination of antibiotic resistance genes (args) in clinically important pathogens. although the args are common in the oral cavity the association of integrons and antibiotic resistance has not been reported there. in this work, a pcr-based approach was used to investigate the presence of integrons and associated gene cassettes in human oral m ... | 2016 | 27304457 |
| nonsyndromic localized aggressive periodontitis of primary dentition: a rare case report. | periodontitis is an inflammatory disease of the gingiva and tissues of the periodontium. it is characterized by pocket formation and destruction of supporting alveolar bone. periodontal diseases of aggressive nature are not very common in children. they are usually associated with systemic conditions. the present case report is of a 5-year-old male child who reported with rapid attachment loss and bony defects of the gingiva and supporting structures. his family and medical history gave no contr ... | 2016 | 27307682 |
| quantitative molecular detection of 19 major pathogens in the interdental biofilm of periodontally healthy young adults. | in oral health, the interdental spaces are a real ecological niche for which the body has few or no alternative defenses and where the traditional daily methods for control by disrupting biofilm are not adequate. the interdental spaces are the source of many hypotheses regarding their potential associations with and/or causes of cardiovascular disease, diabetes, chronic kidney disease, degenerative disease, and depression. this pcr study is the first to describe the interdental microbiota in hea ... | 2016 | 27313576 |
| microbiological efficacy of photodynamic therapy as an adjunct to non-surgical periodontal treatment: a clinical trial. | the efficiency of routine scaling and root planning is negatively influenced by the tooth anatomy and residual bacteria all possibly affecting the treatment outcomes in future. the present study compared the microbiologic effectiveness of the photodynamic therapy (pdt) as an adjunctive treatment modality for nonsurgical treatment in chronic periodontitis. | 2016 | 27330709 |
| dental calculus links statistically to angina pectoris: 26-year observational study. | dental infections, such as periodontitis, associate with atherosclerosis and its complications. we studied a cohort followed-up since 1985 for incidence of angina pectoris with the hypothesis that calculus accumulation, proxy for poor oral hygiene, links to this symptom. | 2016 | 27336307 |
| molecular evolution of the oxygen-binding hemerythrin domain. | the evolution of oxygenic photosynthesis during precambrian times entailed the diversification of strategies minimizing reactive oxygen species-associated damage. four families of oxygen-carrier proteins (hemoglobin, hemerythrin and the two non-homologous families of arthropodan and molluscan hemocyanins) are known to have evolved independently the capacity to bind oxygen reversibly, providing cells with strategies to cope with the evolutionary pressure of oxygen accumulation. oxygen-binding hem ... | 2016 | 27336621 |
| variation in hemolytic activity of brachyspira hyodysenteriae strains from pigs. | brachyspira hyodysenteriae is the primary cause of swine dysentery, which is responsible for major economic losses to the pig industry worldwide. the hemolytic activity of 10 b. hyodysenteriae strains isolated from stools of pigs with mild to mucohemorrhagic diarrhea was compared and seven hemolysis associated genes were sequenced. hemolysis induced by these strains varied from strong to near absent. one weakly hemolytic b. hyodysenteriae strain showed sequence changes in five hemolysis associat ... | 2016 | 27338265 |
| [gender-related marker pathogens of periodontal disease in chronic periodontitis]. | by using qpcr system, women as well as men were found to have an equal periodontal pathogen colonization. however, the women are subjected to have a higher risk of chronic periodontitis onsets. women with the chronic periodontitis usually expose an evident hypercolonization with a single pathogen. p. gingivalis is the most prevalent causative agent of the chronic periodontitis in women but not in men. in health as well as in the chronic periodontitis a complex of periodontal pathogens forms such ... | 2016 | 27367192 |
| genomic and metagenomic analysis of diversity-generating retroelements associated with treponema denticola. | diversity-generating retroelements (dgrs) are genetic cassettes that can produce massive protein sequence variation in prokaryotes. presumably dgrs confer selective advantages to their hosts (bacteria or viruses) by generating variants of target genes-typically resulting in target proteins with altered ligand-binding specificity-through a specialized error-prone reverse transcription process. the only extensively studied dgr system is from the bordetella phage bpp-1, although dgrs are predicted ... | 2016 | 27375574 |
| pseudomonas aeruginosa outer membrane vesicles triggered by human mucosal fluid and lysozyme can prime host tissue surfaces for bacterial adhesion. | pseudomonas aeruginosa is a leading cause of human morbidity and mortality that often targets epithelial surfaces. host immunocompromise, or the presence of indwelling medical devices, including contact lenses, can predispose to infection. while medical devices are known to accumulate bacterial biofilms, it is not well understood why resistant epithelial surfaces become susceptible to p. aeruginosa. many bacteria, including p. aeruginosa, release outer membrane vesicles (omvs) in response to str ... | 2016 | 27375592 |
| clinical, radiographic and microbiological evaluation of high level laser therapy, a new photodynamic therapy protocol, in peri-implantitis treatment; a pilot experience. | aim. endosseous implants are widely used to replace missing teeth but mucositis and peri-implantitis are the most frequent long-term complications related with dental implants. removing all bacterial deposits on contaminated implant surface is very difficult due to implant surface morphology. the aim of this study was to evaluate the bactericidal potential of photodynamic therapy by using a new high level laser irradiation protocol associated with hydrogen peroxide in peri-implantitis. materials ... | 2016 | 27379251 |
| peri-implant disease: what we know and what we need to know. | peri-implant disease is a serious problem that plagues today's dentistry, both in terms of therapy and epidemiology. with the expansion of the practice of implantology and an increasing number of implants placed annually, the frequency of peri-implant disease has greatly expanded. its clinical manifestations, in the absence of a globally established classification, are peri-implant mucositis and peri-implantitis, the counterparts of gingivitis and periodontitis, respectively. however, many doubt ... | 2016 | 27382503 |
| frequency of periodontal pathogens and helicobacter pylori in the mouths and stomachs of obese individuals submitted to bariatric surgery: a cross-sectional study. | this cross-sectional study compared the frequency of oral periodontopathogens and h. pylori in the mouths and stomachs of obese individuals with or without periodontitis submitted to bariatric surgery. | 2016 | 27383704 |
| porphyromonas gingivalis-induced mir-132 regulates tnfα expression in thp-1 derived macrophages. | periodontitis is a chronic inflammatory disease induced by periodontopathogens such as porphyromonas gingivalis (p. gingivalis). micrornas (mirnas) are small single-stranded noncoding rnas that regulate gene expression at the level of translation. mirnas have been reported to be involved in inflammatory processes. in this study, we examined the effects of p. gingivalis-induced inflammatory mirnas expression on tnfα production in thp-1 derived macrophages. | 2016 | 27386246 |
| role of porphyromonas gingivalis hmuy in immunopathogenesis of chronic periodontitis. | periodontitis is a multifactorial disease, with participation of bacterial, environmental, and host factors. it results from synergistic and dysbiotic multispecies microorganisms, critical "keystone pathogens," affecting the whole bacterial community. the purpose of this study was to review the role of porphyromonas gingivalis in the immunopathogenesis of chronic periodontitis, with special attention paid to hmuy. the host response during periodontitis involves the innate and adaptive immune sys ... | 2016 | 27403039 |
| periodontal bacterial colonization in synovial tissues exacerbates collagen-induced arthritis in b10.riii mice. | it has been previously hypothesized that oral microbes may be an etiological link between rheumatoid arthritis (ra) and periodontal disease. however, the mechanistic basis of this association is incompletely understood. here, we investigated the role of periodontal bacteria in induction of joint inflammation in collagen-induced arthritis (cia) in b10.riii mice. | 2016 | 27405639 |
| the genomic sequence of the oral pathobiont strain ni1060 reveals unique strategies for bacterial competition and pathogenicity. | strain ni1060 is an oral bacterium responsible for periodontitis in a murine ligature-induced disease model. to better understand its pathogenicity, we have determined the complete sequence of its 2,553,982 bp genome. although closely related to pasteurella pneumotropica, a pneumonia-associated rodent commensal based on its 16s rrna, the ni1060 genomic content suggests that they are different species thriving on different energy sources via alternative metabolic pathways. genomic and phylogeneti ... | 2016 | 27409077 |
| treatment of a periodontic-endodontic lesion in a patient with aggressive periodontitis. | case description. this case report describes the successful management of a left mandibular first molar with a combined periodontic-endodontic lesion in a 35-year-old caucasian woman with aggressive periodontitis using a concerted approach including endodontic treatment, periodontal therapy, and a periodontal regenerative procedure using an enamel matrix derivate. in spite of anticipated poor prognosis, the tooth lesion healed. this case report also discusses the rationale behind different treat ... | 2016 | 27418983 |
| the current weight of evidence of the microbiologic profile associated with peri-implantitis: a systematic review. | there is currently no consensus regarding microorganisms that may be considered true peri-implant pathogens. therefore, the aim of this systematic review is to determine the weight of evidence for microorganisms related to peri-implantitis based on results of association studies. | 2016 | 27420109 |
| characterization of a potential abc-type bacteriocin exporter protein from treponema denticola. | treponema denticola is strongly associated with the development of periodontal disease. both synergistic and antagonistic effects are observed among bacterial species in the process of biofilm formation. bacteriocin-related genes have not yet been fully characterized in periodontopathic bacteria. the aim of this study was to detect and characterize bacteriocin-associated proteins in t. denticola. | 2016 | 27422166 |
| characterization of a potential abc-type bacteriocin exporter protein from treponema denticola. | treponema denticola is strongly associated with the development of periodontal disease. both synergistic and antagonistic effects are observed among bacterial species in the process of biofilm formation. bacteriocin-related genes have not yet been fully characterized in periodontopathic bacteria. the aim of this study was to detect and characterize bacteriocin-associated proteins in t. denticola. | 2016 | 27422166 |
| epigenetic switch driven by dna inversions dictates phase variation in streptococcus pneumoniae. | dna methylation is an important epigenetic mechanism for phenotypic diversification in all forms of life. we previously described remarkable cell-to-cell heterogeneity in epigenetic pattern within a clonal population of streptococcus pneumoniae, a leading human pathogen. we here report that the epigenetic diversity is caused by extensive dna inversions among hsdsa, hsdsb, and hsdsc, three methyltransferase hsds genes in the spn556ii type-i restriction modification (r-m) locus. because hsdsa enco ... | 2016 | 27427949 |
| micrornas as salivary markers for periodontal diseases: a new diagnostic approach? | the aim of this review is to discuss current findings regarding the roles of mirnas in periodontal diseases and the potential use of saliva as a diagnostic medium for corresponding mirna investigations. for periodontal disease, investigations have been restricted to tissue samples and five mirnas, that is, mir-142-3p, mir-146a, mir-155, mir-203, and mir-223, were repeatedly validated in vivo and in vitro by different validation methods. particularly noticeable are the small sample sizes, differe ... | 2016 | 27429973 |
| diurnal dynamic behavior of microglia in response to infected bacteria through the udp-p2y6 receptor system. | it has long been believed that microglia morphologically transform into the activated state by retracting their long processes and consuming pathogens when bacteria infect into the brain parenchyma. in the present study, however, we showed for the first time that murine cortical microglia extend their processes towards focally injected porphyromonas gingivalis. this p. gingivalis-induced microglial process extension was significantly increased during the light (sleeping) phase than the dark (wak ... | 2016 | 27445174 |
| the use of pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) to detect bacterial and fungal colonization in healthy military service members. | the role of microbial colonization in disease is complex. novel molecular tools to detect colonization offer theoretical improvements over traditional methods. we evaluated pcr/electrospray ionization-time-of-flight-mass spectrometry (pcr/esi-tof-ms) as a screening tool to study colonization of healthy military service members. | 2016 | 27448413 |
| impact of implant-abutment connection on osteoimmunological and microbiological parameters in short implants: a randomized controlled clinical trial. | the study aimed to determine the levels of soluble receptor activator of nuclear factor-кb ligand (srankl) and osteoprotegerin (opg) as well as their relative calculated ratio in peri-implant crevicular fluid (picf) obtained around two different types of implant-abutment connection on short implants following a 12-month monitoring period. moreover, the levels of a number of oral bacterial species were investigated in the corresponding submucosal biofilm samples. | 2016 | 27469171 |
| evaluation of the efficacy of a new oral gel as an adjunct to home oral hygiene in the management of chronic periodontitis. a microbiological study using pcr analysis. | the use of chemical devices for non-surgical periodontal therapy has led to new treatment strategies aiming primarily at infection control and oral bacterial load. over the last few decades adjunctive chemical devices has been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of a new oral gel named parodongel on the red complex organisms using polymerase chain reaction (pcr) for microbiological analysis. a total of 10 patients with a dia ... | 2016 | 27469558 |
| evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study. | the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ... | 2016 | 27469559 |
| bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas. | the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ... | 2016 | 27469562 |
| bacteria prevalence in a large italian population sample: a clinical and microbiological study. | the present study detects those bacterial species which are more strongly related to bleeding on probing, suppuration and smoking in periodontal-affected patients. nine hundred and fifty-one patients with periodontal diseases were admitted to the department of periodontology and implantology, dental school of bologna university where they underwent microbiological tests for six periodontal pathogens (actinomyces actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, treponema de ... | 2016 | 27469569 |
| mechanistic and evolutionary insights from the reciprocal promiscuity of two pyridoxal phosphate-dependent enzymes. | enzymes that utilize the cofactor pyridoxal 5'-phosphate play essential roles in amino acid metabolism in all organisms. the cofactor is used by proteins that adopt at least five different folds, which raises questions about the evolutionary processes that might explain the observed distribution of functions among folds. in this study, we show that a representative of fold type iii, the escherichia coli alanine racemase (alr), is a promiscuous cystathionine β-lyase (cbl). furthermore, e. coli cb ... | 2016 | 27474741 |
| oral, intestinal, and skin bacteria in ventral hernia mesh implants. | in ventral hernia surgery, mesh implants are used to reduce recurrence. infection after mesh implantation can be a problem and rates around 6-10% have been reported. bacterial colonization of mesh implants in patients without clinical signs of infection has not been thoroughly investigated. molecular techniques have proven effective in demonstrating bacterial diversity in various environments and are able to identify bacteria on a gene-specific level. | 2016 | 27476443 |
| three coa transferases involved in the production of short chain fatty acids in porphyromonas gingivalis. | butyryl-coa:acetate coa transferase, which produces butyrate and acetyl-coa from butyryl-coa and acetate, is responsible for the final step of butyrate production in bacteria. this study demonstrates that in the periodontopathogenic bacterium porphyromonas gingivalis this reaction is not catalyzed by pgn_1171, previously annotated as butyryl-coa:acetate coa transferase, but by three distinct coa transferases, pgn_0725, pgn_1341, and pgn_1888. gas chromatography/mass spectrometry (gc-ms) and spec ... | 2016 | 27486457 |
| microbiota and chronic inflammatory arthritis: an interwoven link. | only recently, the scientific community gained insights on the importance of the intestinal resident flora for the host's health and disease. gut microbiota in fact plays a crucial role in modulating innate and acquired immune responses and thus interferes with the fragile balance inflammation versus tolerance. | 2016 | 27492386 |
| distinct interacting core taxa in co-occurrence networks enable discrimination of polymicrobial oral diseases with similar symptoms. | polymicrobial diseases, which can be life threatening, are caused by the presence and interactions of multiple microbes. peri-implantitis and periodontitis are representative polymicrobial diseases that show similar clinical symptoms. to establish a means of differentiating between them, we compared microbial species and functional genes in situ by performing metatranscriptomic analyses of peri-implantitis and periodontitis samples obtained from the same subjects (n = 12 each). although the two ... | 2016 | 27499042 |
| minocycline ointment as a local drug delivery in the treatment of generalized chronic periodontitis - a clinical study. | the primary goal in periodontal therapy includes removal of the etiological factors by mechanical periodontal treatment, which sometimes fail to eliminate the anaerobic infection at the base of the pocket and requires adjuvant chemical therapy. | 2016 | 27504402 |
| distinguishing the signals of gingivitis and periodontitis in supragingival plaque: a cross-sectional cohort study in malawi. | periodontal disease ranges from gingival inflammation (gingivitis) to the inflammation and loss of tooth-supporting tissues (periodontitis). previous research has focused mainly on subgingival plaque, but supragingival plaque composition is also known to be associated with disease. quantitative modeling of bacterial abundances across the natural range of periodontal severities can distinguish which features of disease are associated with particular changes in composition. we assessed a cross-sec ... | 2016 | 27520811 |
| two different virulence-related regulatory pathways in borrelia burgdorferi are directly affected by osmotic fluxes in the blood meal of feeding ixodes ticks. | lyme disease, caused by borrelia burgdorferi, is a vector-borne illness that requires the bacteria to adapt to distinctly different environments in its tick vector and various mammalian hosts. effective colonization (acquisition phase) of a tick requires the bacteria to adapt to tick midgut physiology. successful transmission (transmission phase) to a mammal requires the bacteria to sense and respond to the midgut environmental cues and up-regulate key virulence factors before transmission to a ... | 2016 | 27525653 |
| oral behavior, dental, periodontal and microbiological findings in patients undergoing hemodialysis and after kidney transplantation. | aim of this single center cross-sectional study was to investigate oral behavior, dental, periodontal and microbiological findings in patients undergoing hemodialysis (hd) and after kidney transplantation (kt). | 2016 | 27531498 |
| impact of aging on trem-1 responses in the periodontium: a cross-sectional study in an elderly population. | aging is associated with altered immune response, which increases susceptibility to infections. strem-1 is involved in the amplification of the inflammatory response to bacterial infection. the present cross-sectional study aims to investigate local strem-1 levels in gingival crevicular fluid (gcf) as well as key periodontal pathogen levels in the subgingival plaque in an elderly cohort with periodontal health, gingivitis, and chronic periodontitis (cp). | 2016 | 27542376 |
| essential role of the linker region in the higher catalytic efficiency of a bifunctional msra-msrb fusion protein. | many bacteria, particularly pathogens, possess methionine sulfoxide reductase a (msra) and b (msrb) as a fusion form (msrab). however, it is not clear why they possess a fusion msrab form rather than the separate enzymes that exist in most organisms. in this study, we performed biochemical and kinetic analyses of msrab from treponema denticola (tdmsrab), single-domain forms (tdmsra and tdmsrb), and catalytic cys mutants (tdmsrab(c11s) and tdmsrab(c285s)). we found that the catalytic efficiency o ... | 2016 | 27551953 |
| longitudinal study on clinical and microbial analysis of periodontal status in pregnancy. | this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ... | 2016 | 27556678 |
| immunologic characteristics of human gingival fibroblasts in response to oral bacteria. | there is ample evidence that gingival fibroblasts (gfs) participate in the immune response to oral bacteria and serve as immune-regulatory cells. the objective of this study was to investigate the innate immune response of gfs to oral bacteria. | 2016 | 27558278 |
| persistence of porphyromonas gingivalis is a negative predictor in patients with moderate to severe periodontitis after nonsurgical periodontal therapy. | the aim of this study was to evaluate the quality of prediction for stable results after nonsurgical periodontal therapy by several microbiological variables of the subgingival biofilm and biomarkers of gingival crevicular fluid or oral lavage. | 2017 | 27558382 |
| periodontitis contributes to adipose tissue inflammation through the nf-<kappa>b, jnk and erk pathways to promote insulin resistance in a rat model. | this study aimed to investigate the mechanism by which periodontitis affects the inflammatory response and systemic insulin resistance in the white adipose and liver tissues in an obese rat model. the obese model was generated by feeding rats a high fat diet. the periodontitis model was induced by ligatures and injection of "red complex", which consisted of porphyromonas gingivalis, treponema denticola, and tannerella forsythia, for two weeks. when compared with rats without periodontitis, fasti ... | 2016 | 27565999 |
| conservation of the c-type lectin fold for accommodating massive sequence variation in archaeal diversity-generating retroelements. | diversity-generating retroelements (dgrs) provide organisms with a unique means for adaptation to a dynamic environment through massive protein sequence variation. the potential scope of this variation exceeds that of the vertebrate adaptive immune system. dgrs were known to exist only in viruses and bacteria until their recent discovery in archaea belonging to the 'microbial dark matter', specifically in organisms closely related to nanoarchaeota. however, nanoarchaeota dgr variable proteins we ... | 2016 | 27578274 |
| cloning, purification and characterization of the collagenase cola expressed by bacillus cereus atcc 14579. | bacterial collagenases differ considerably in their structure and functions. the collagenases colh and colg from clostridium histolyticum and cola expressed by clostridium perfringens are well-characterized collagenases that cleave triple-helical collagen, which were therefore termed as ´true´ collagenases. cola from bacillus cereus (b. cereus) has been added to the collection of true collagenases. however, the molecular characteristics of b. cereus cola are less understood. in this study, we id ... | 2016 | 27588686 |
| the role of nutrition in periodontal health: an update. | periodontal health is influenced by a number of factors such as oral hygiene, genetic and epigenetic factors, systemic health, and nutrition. many studies have observed that a balanced diet has an essential role in maintaining periodontal health. additionally, the influences of nutritional supplements and dietary components have been known to affect healing after periodontal surgery. studies have attempted to find a correlation between tooth loss, periodontal health, and nutrition. moreover, bon ... | 2016 | 27589794 |
| rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis. | high-speed capillary electrophoresis (hsce) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). in present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (sdis) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (porphyromonas gingivalis (p.g), treponema denticola (t.d) and tannerela forsythia (t.f)). the reliabilit ... | 2016 | 27591633 |
| integrating inflammasome signaling in sexually transmitted infections. | inflammasomes are cytosolic multiprotein platforms with pivotal roles in infectious diseases. activation of inflammasomes results in proinflammatory cytokine signaling and pyroptosis. sexually transmitted infections (stis) are a major health problem worldwide, yet few studies have probed the impact of inflammasome signaling during these infections. due to the dearth of appropriate infection models, our current understanding of inflammasomes in stis is mostly drawn from results obtained in vitro, ... | 2016 | 27592079 |
| effect of helicobacter pylori infection on chronic periodontitis by the change of microecology and inflammation. | helicobacter pylori (h. pylori), a pathogen inducing peptic disease, is recently found to be binding to the progress of periodontitis. most previous studies are case-controlled, and they investigate the risk of h. pylori infection in disease the development of while few studies evaluate the correlation between h. pylori and periodontal pathogens. therefore, we investigated the correlation between h. pylori infection with periodontal parameters, periodontal pathogens and inflammation. the results ... | 2016 | 27602578 |
| biased and unbiased methods for the detection of off-target cleavage by crispr/cas9: an overview. | the clustered regularly interspaced short palindromic repeat (crispr)-associated protein 9 endonuclease (cas9) derived from bacterial adaptive immune systems is a revolutionary tool used in both basic and applied science. it is a versatile system that enables the genome of different species to be modified by generating double strand breaks (dsbs) at specific locations. however, all of the crispr/cas9 systems can also produce dsbs at off-target sites that differ substantially from on-target sites ... | 2016 | 27618019 |
| herpesviral-bacterial co-infection in mandibular third molar pericoronitis. | the aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis. | 2016 | 27620215 |
| the distribution of porphyromonas gingivalis, tannerella forsythia, treponema denticola and aggregatibacter actinomycetemcomitans in patients with alcoholic disease: a pilot study. | both drinking and periodontal disease are serious health and social problems. findings on the effect of alcohol consumption on periodontal disease are inconclusive. | 2016 | 27627556 |
| a double-blind randomized trial comparing implants with laser-microtextured and machined collar surfaces: microbiologic and clinical results. | the purpose of this study was to compare the clinical outcome of and determine the differences in periodonto-pathogenic microbiota around two types of implant collar surfaces: laser-microtextured (test) vs machined (control). | 2017 | 27632268 |
| dental calculus stimulates interleukin-1β secretion by activating nlrp3 inflammasome in human and mouse phagocytes. | dental calculus is a mineralized deposit associated with periodontitis. the bacterial components contained in dental calculus can be recognized by host immune sensors, such as toll-like receptors (tlrs), and induce transcription of proinflammatory cytokines, such as il-1β. studies have shown that cellular uptake of crystalline particles may trigger nlrp3 inflammasome activation, leading to the cleavage of the il-1β precursor to its mature form. phagocytosis of dental calculus in the periodontal ... | 2016 | 27632566 |