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high-throughput-sequencing-based identification of a grapevine fanleaf virus satellite rna in vitis vinifera.a new satellite rna (satrna) of grapevine fanleaf virus (gflv) was identified by high-throughput sequencing of high-definition (hd) adapter libraries from grapevine plants of the cultivar panse precoce (ppe) affected by enation disease. the complete nucleotide sequence was obtained by automatic sequencing using primers designed based on next-generation sequencing (ngs) data. the full-length sequence, named satgflv-ppe, consisted of 1119 nucleotides with a single open reading frame from position ...201626873812
the contribution of flowering time and seed content to uneven ripening initiation among fruits within vitis vinifera l. cv. pinot noir clusters.ripening initiation-associated hormonal changes and sugar accumulation for individual fruits differed by seed content and did not depend heavily on flowering time or duration from anthesis to clusters' onset of ripening. for vitis vinifera, the ripening initiation of individual fruits in a cluster occurs unevenly. this developmental period is called véraison. why individual fruits initiate ripening at different times is not well studied, though differences in seed content and unequal development ...201626874729
characterization of triterpenoid profiles and triterpene synthase expression in the leaves of eight vitis vinifera cultivars grown in the upper rhine valley.plant triterpenoids are a diverse group of secondary metabolites with wide distribution, high chemical diversity and interesting pharmacological and antimicrobial properties. the first step in the biosynthesis of all triterpenoids is the cyclization of the 2,3-oxidosqualene precursor, catalyzed by oxidosqualene cyclases (oscs), which have characteristic product specificities. biosynthesis and functions of pentacyclic triterpenes have been poorly studied in grapevine. in this study, we first inve ...201626879930
direct ingestion method for enhancing production and bioavailability of resveratrol and other phytoalexins in vitis vinifera.phytoalexins such as resveratrol and pterostilbene, produced de novo by many plant species, including grapevine (vitis vinifera), play a role in plant defense against injury and pathogens. in human cell lines and in animal studies, phytoalexins have been shown to be highly beneficial, with protective effects against cancer, cardiovascular disease, neurodegenerative diseases, diabetes, hyperglycemia, as well as potential effects on longevity. however, in clinical studies, there are multiple facto ...201626880624
influence of berry ripeness on accumulation, composition and extractability of skin and seed flavonoids in cv. sangiovese (vitis vinifera l.).the anthocyanin and tannin concentration and composition of vitis vinifera l. cv. sangiovese berries were investigated from post-veraison to harvest. exhaustive extraction with methanol and acetone was performed to determine the total flavonoid concentration, while a model hydroalcoholic solution was used to prepare extracts representing the winemaking process. the aim of this study was to improve the knowledge of the phenolic maturity of sangiovese grape.201626888489
bio-conjugation of antioxidant peptide on surface-modified gold nanoparticles: a novel approach to enhance the radical scavenging property in cancer cell.functionalized gold nanoparticles are emerging as a promising nanocarrier for target specific delivery of the therapeutic molecules in a cancer cell, as a result it targeted selectively to the cancer cell and minimized the off-target effect. the functionalized nanomaterial (bio conjugate) brings novel functional properties, for example, the high payload of anticancer, antioxidant molecules and selective targeting of the cancer molecular markers. the current study reported the synthesis of multif ...201626900409
predictive method for correct identification of archaeological charred grape seeds: support for advances in knowledge of grape domestication process.the identification of archaeological charred grape seeds is a difficult task due to the alteration of the morphological seeds shape. in archaeobotanical studies, for the correct discrimination between vitis vinifera subsp. sylvestris and vitis vinifera subsp. vinifera grape seeds it is very important to understand the history and origin of the domesticated grapevine. in this work, different carbonisation experiments were carried out using a hearth to reproduce the same burning conditions that oc ...201626901361
bone health nutraceuticals alter microarray mrna gene expression: a randomized, parallel, open-label clinical study.dietary intake of fruits and vegetables has been suggested to have a role in promoting bone health. more specifically, the polyphenols they contain have been linked to physiological effects related to bone mineral density and bone metabolism. in this research, we use standard microarray analyses of peripheral whole blood from post-menopausal women treated with two fixed combinations of plant extracts standardized to polyphenol content to identify differentially expressed genes relevant to bone h ...026902403
application of 2d and 3d image technologies to characterise morphological attributes of grapevine clusters.grapevine cluster morphology influences the quality and commercial value of wine and table grapes. it is routinely evaluated by subjective and inaccurate methods that do not meet the requirements set by the food industry. novel two-dimensional (2d) and three-dimensional (3d) machine vision technologies emerge as promising tools for its automatic and fast evaluation.201626910811
assessment of injuries caused by anastrepha fraterculus (wied.) (diptera: tephritidae) on the incidence of bunch rot diseases in table grape.anastrepha fraterculus (wied.) is the main insect pest of table grapes (vitis vinifera) in the southern region of brazil. in this study, we aimed to investigate the effect of fruit puncturing by adult females and larval infestation by a. fraterculus on the occurrence of bunch rot disease in the grape (cultivar "itália") by evaluating grapes (a) punctured for oviposition by females of a. fraterculus, sterilized in laboratory with novaluron (40 mg l(-1)) and further spray-inoculated separately wit ...201626911161
impacts of grapevine leafroll disease on fruit yield and grape and wine chemistry in a wine grape (vitis vinifera l.) cultivar.grapevine leafroll disease (gld) is an economically important virus disease affecting wine grapes (vitis vinifera l.), but little is known about its effect on wine chemistry and sensory composition of wines. in this study, impacts of gld on fruit yield, berry quality and wine chemistry and sensory features were investigated in a red wine grape cultivar planted in a commercial vineyard. own-rooted merlot vines showing gld symptoms and tested positive for grapevine leafroll-associated virus 3 and ...201626919614
authentication of punica granatum l.: development of scar markers for the detection of 10 fruits potentially used in economically motivated adulteration.the large commercial success of pomegranate increase the likelihood of economically motivated adulteration (ema), which has been gradually spotted with the undeclared addition of anthocyanin-rich plants or cheaper fruit juices used as bulking and diluting agents. a method based on sequence-characterized amplified regions (scars) was developed to detect the presence of aristotelia chilensis, aronia melanocarpa, dioscorea alata, euterpe oleracea, malus×domestica, morus nigra, sambucus nigra, vacci ...201626920316
methoxypyrazine accumulation and o-methyltransferase gene expression in sauvignon blanc grapes: the role of leaf removal, light exposure, and berry development.methoxypyrazines are present in the grapes of certain vitis vinifera varieties including sauvignon blanc and contribute herbaceous/green aromas to wine. environmental factors such as light exposure and temperature can influence methoxypyrazine levels, and viticultural interventions such as canopy manipulation have the ability to reduce methoxypyrazine accumulation in grapes. we assessed methoxypyrazine levels and showed that leaf removal significantly reduces accumulation in sauvignon blanc grap ...201626923868
alterations in primary and secondary metabolism in vitis vinifera 'malvasía de banyalbufar' upon infection with grapevine leafroll-associated virus 3.plant defense mechanisms against pathogens result in differential regulation of various processes of primary and secondary metabolism. imaging techniques, such as fluorescence imaging and thermography, are very valuable tools providing spatial and temporal information about these processes. in this study, effects of grapevine leafroll-associated virus 3 (glrav-3) on grapevine physiology were analyzed in pot-grown asymptomatic plants of the white cultivar malvasía de banyalbufar. the virus trigge ...201626926417
vvmje1 of the grapevine (vitis vinifera) vvmes methylesterase family encodes for methyl jasmonate esterase and has a role in stress response.the known members of plant methyl esterase (mes) family catalyze the hydrolysis of a c-o ester linkage of methyl esters of several phytohormones including indole-3-acetic acid, salicylic acid and jasmonic acid. the genome of grapevine (vitis vinifera) was found to contain 15 mes genes, designated vvmes1-15. in this report, vvmes5 was selected for molecular, biochemical and structural studies. vvmes5 is most similar to tomato methyl jasmonate esterase. e. coli-expressed recombinant vvmes5 display ...201626934101
high temperature affects photosynthetic and molecular processes in field-cultivated vitis vinifera l. × vitis labrusca l.high-temperature stress markedly influences grape growth and development. however, how high-temperature stress response differs between controlled and field-cultivated grape is poorly understood. in this study, the effects of heat treatment on grapevines were studied for changes in photosystem ii (psii) activity and expression levels of heat-responsive genes and heat shock protein hsp21. july 31st, 2015 was considered as the post high-temperature treatment ("42°c"; temperatures above 40°c for a ...201626946321
production of highly bioactive resveratrol analogues pterostilbene and piceatannol in metabolically engineered grapevine cell cultures.grapevine stilbenes, particularly trans-resveratrol, have a demonstrated pharmacological activity. other natural stilbenes derived from resveratrol such as pterostilbene or piceatannol, display higher oral bioavailability and bioactivity than the parent compound, but are far less abundant in natural sources. thus, to efficiently obtain these bioactive resveratrol derivatives, there is a need to develop new bioproduction systems. grapevine cell cultures are able to produce large amounts of easily ...201626947765
relationship between the elemental composition of grapeyards and bioactive compounds in the cabernet sauvignon grapes vitis vinífera harvested in mexico.the red grape vitis vinífera is an important source of phenolic compounds, which can prevent disease if included as a part of a diet. the levels of these compounds in grapes have been associated with various environmental factors, such as climate, soil composition, and biotic stress. the purpose of this study was to determine the relationship between the elemental compositions of the soil and the grapes and the presence of bioactive compounds, such as catechin, epicatechin, piceid and resveratro ...201626948592
effect of irrigation regime on perceived astringency and proanthocyanidin composition of skins and seeds of vitis vinifera l. cv. syrah grapes under semiarid conditions.in this work, the effect of water availability on astringency of seed and skin extracts of vitis vinifera cv. syrah berries under the typical semiarid conditions of greece was investigated. moreover, astringency was assessed in relation to proanthocyanidin composition. for this purpose, three irrigation treatments were applied starting at berry set through harvest of 2011 and 2012: full irrigation (fi) at 100% of crop evapotranspiration, deficit irrigation (di) at 50% and non-irrigated (ni). fi ...201626948617
geochemical characterization of elements in vitis vinifera cv. negroamaro grape berries grown under different soil managements.the present geochemical study concerns the impact of viticultural practices in the chemical composition of the grape cultivar "negroamaro" in apulia, a southern italian region renowned for its quality wine. three types of soil management (sm), two cover cropping with different mixtures, and a soil tillage were considered. for each sm, the vines were irrigated according to two irrigation levels. chemical composition of soil and of berries of vitis vinifera cultivar "negroamaro" were analyzed by x ...201626951448
data on antioxidant activity in grapevine (vitis vinifera l.) following cryopreservation by vitrification.cryopreservation is used for the long-term conservation of plant genetic resources. this technique very often induces lethal injury or tissue damage. in this study, we measured indicators of viability and cell damage following cryopreservation and vitrification-cryopreservation in vitis vinifera l. axillary buds cv. "flame seedless" stored in liquid nitrogen (ln) for: three seconds, one hour, one day, one week and one month; after ln thawed at 38 °c for three minutes. the enzymatic activity of c ...201526958607
grapevine red blotch-associated virus is present in free-living vitis spp. proximal to cultivated grapevines.red blotch is an emerging disease of grapevine associated with grapevine red blotch-associated virus (grbav). the virus spreads with infected planting stocks but no vector of epidemiological significance has been conclusively identified. a vineyard block of red-blotch-affected vitis vinifera 'cabernet franc' clone 214 was observed in california, with a clustering of infected, symptomatic vines focused along one edge of the field proximal to a riparian habitat with free-living vitis spp. no genet ...201626960112
rapidly changing climatic conditions for wine grape growing in the okanagan valley region of british columbia, canada.a statistical analysis was conducted on long-term climate records for sites bordering okanagan lake in the okanagan valley viticultural region of british columbia, canada. average wine grape growing season temperatures are increasing rapidly in the area over the post-1980 period at rates upwards of 7.0±1.3°c/century. similar increases in the average dormant season temperature are evident. these temperature changes are likely some of the most extreme observed among the world's wine producing area ...201626971218
comparison of cbf1, cbf2, cbf3 and cbf4 expression in some grapevine cultivars and species under cold stress.grapevine, an important horticultural crop in the world, is moderately tolerant to cold conditions and is subjected to the cold injuries at different regions. so studies on different aspects of tolerance mechanism to unexpected cold of late spring as well as winter freezing seems necessary about this vine. for this reason, study on genes responsible for acquiring cold tolerance is very important. transcription factors are among regulatory proteins that are responsible for cold acclimation. in th ...201526973374
influence of berry heterogeneity on phenolics and antioxidant activity of grapes and wines: a primary study of the new winegrape cultivar meili (vitis vinifera l.).wine grapes are usually harvested in vineyards when they ripen. however, not all of the berries in a vineyard ripen homogeneously because of different microclimates around the clusters and berries. in this study, the influence of berry heterogeneity on the phenolic content and antioxidant capacity of grapes and wines under a continental monsoon climate was evaluated for a new wine grape cultivar meili (vitis vinifera l.). the total phenolic, flavonoid, flavanol, and monomeric anthocyanin content ...201626974974
molecular evolution and gene expression differences within the hd-zip transcription factor family of zea mays l.homeodomain-leucine zipper (hd-zip) transcription factors regulate developmental processes and stress responses in plants, and they vary widely in gene number and family structure. in this study, 55 predicted maize hd-zip genes were systematically analyzed with respect to their phylogenetic relationships, molecular evolution, and gene expression in order to understand the functional diversification within the family. phylogenetic analysis of hd-zip proteins from zea mays, oryza sativa, arabidops ...201626979310
in vitro extraction and fermentation of polyphenols from grape seeds (vitis vinifera) by human intestinal microbiota.the effects of several parameters on the extraction yield of total polyphenols from grape seeds by pressurized liquid extraction were investigated. the highest recovery of total polyphenols occurred at 80 °c within 5 min, and a single extraction allowed a recovery of more than 97% of total polyphenols. following the purification with macroporous resin, the effects of grape polyphenols (>94.8%) on human intestinal microbiota were monitored over 36 h incubation by fluorescence in situ hybridizatio ...201626980065
terpene evolution during the development of vitis vinifera l. cv. shiraz grapes.the flavour of wine is derived, in part, from the flavour compounds present in the grape, which change as the grapes accumulate sugar and ripen. grape berry terpene concentrations may vary at different stages of berry development. this study aimed to investigate terpene evolution in grape berries from four weeks post-flowering to maturity. grape bunches were sampled at fortnightly intervals over two vintages (2012-13 and 2013-14). in total, five monoterpenoids, 24 sesquiterpenes, and four noriso ...201626988525
influence of genetic and vintage factors in flavan-3-ol composition of grape seeds of a segregating vitis vinifera population.flavan-3-ol compounds are important secondary metabolites which show high antioxidant activity and are responsible for bitterness and astringency of food products. the aim of this work was to evaluate the potential for selecting grape materials with higher seed flavanol content from a breeding population. the composition and content of flavan-3-ols from 151 genotypes obtained from crossing wine grape varieties was evaluated by uplc in three consecutive years.201726992139
bio-based extraction and stabilization of anthocyanins.this work reports a novel method of recovering anthocyanin compounds from highly-pigmented grapes via a fermentation based approach. it was hypothesized that batch growth of zymomonas mobilis on simple medium would produce both ethanol and enzymes/biomass-acting materials, the combination of which will provide a superior extraction when compared to simple alcohol extraction. to examine this hypothesis, z. mobilis was fermented in a batch consisting of mashed vitis vinifera and glucose, and the r ...201626996151
transcriptome and metabolite profiling reveals that prolonged drought modulates the phenylpropanoid and terpenoid pathway in white grapes (vitis vinifera l.).secondary metabolism contributes to the adaptation of a plant to its environment. in wine grapes, fruit secondary metabolism largely determines wine quality. climate change is predicted to exacerbate drought events in several viticultural areas, potentially affecting the wine quality. in red grapes, water deficit modulates flavonoid accumulation, leading to major quantitative and compositional changes in the profile of the anthocyanin pigments; in white grapes, the effect of water deficit on sec ...201627001212
abscisic acid transcriptomic signaling varies with grapevine organ.abscisic acid (aba) regulates various developmental processes and stress responses over both short (i.e. hours or days) and longer (i.e. months or seasons) time frames. to elucidate the transcriptional regulation of early responses of grapevine (vitis vinifera) responding to aba, different organs of grape (berries, shoot tips, leaves, roots and cell cultures) were treated with 10 μm (s)-(+)-aba for 2 h. nimblegen whole genome microarrays of vitis vinifera were used to determine the effects of ab ...201627001301
vitis vinifera seeds extract for the modulation of cytosolic factors bax-α and nf-kb involved in uvb-induced oxidative stress and apoptosis of human skin cells.the depletion of the ozone layer allows overexposure of the skin to uv radiation, which is prolonged due to the increasing life expectancy, together with inappropriate life habits contribute to the increasing incidence of cutaneous malignancies. plant extracts with antioxidant capacities are frequently employed as a means to protect skin against ultraviolet (uv) radiations, thus preventing skin cancers. in the present study we assessed a red grape seed extract (gse) potential capacities to reduc ...201627004028
genetic diversity, linkage disequilibrium and power of a large grapevine (vitis vinifera l) diversity panel newly designed for association studies.as for many crops, new high-quality grapevine varieties requiring less pesticide and adapted to climate change are needed. in perennial species, breeding is a long process which can be speeded up by gaining knowledge about quantitative trait loci linked to agronomic traits variation. however, due to the long juvenile period of these species, establishing numerous highly recombinant populations for high resolution mapping is both costly and time-consuming. genome wide association studies in germp ...201627005772
phosphoproteomic analysis of induced resistance reveals activation of signal transduction processes by beneficial and pathogenic interaction in grapevine.protein phosphorylation regulates several key processes of the plant immune system. protein kinases and phosphatases are pivotal regulators of defense mechanisms elicited by resistance inducers. however, the phosphorylation cascades that trigger the induced resistance mechanisms in plants have not yet been deeply investigated. the beneficial fungus trichoderma harzianum t39 (t39) induces resistance against grapevine downy mildew (plasmopara viticola), but its efficacy could be further improved b ...201627010348
variations in early response of grapevine wood depending on wound and inoculation combinations with phaeoacremonium aleophilum and phaeomoniella chlamydospora.defense mechanisms in woody tissue are poorly understood, especially in vine colonized by trunk pathogens. however, several investigations suggest that molecular mechanisms in the central tissue of vitis vinifera l. may be involved in trunk-defense reactions. in this work, the perception of phaeoacremonium aleophilum and phaeomoniella chlamydospora alone or together were investigated in cuttings of cabernet sauvignon trunks. plant responses were analyzed at the tissue level via optical microscop ...201627014294
reproductive patterns of xylotrechus arvicola (coleoptera: cerambycidae), an emerging pest of grape-vines, under laboratory conditions.xylotrechus arvicola(olivier) is an emerging pest in vineyards (vitis vinifera) in the iberian peninsula. its reproductive biology has been studied in the laboratory in order to develop pest-control measures. obtained results show that the oviposition period is significantly longer in wild females (16.6 ± 2.9 d) than in laboratory females (9.7 ± 1.3 d), but there are no significant differences in the egg-hatching periods between the wild and the laboratory females. fecundity is significantly hig ...201627016597
ageing-associated oxidative stress and inflammation are alleviated by products from grapes.advanced age is associated with increased incidence of a variety of chronic disease states which share oxidative stress and inflammation as causative role players. furthermore, data point to a role for both cumulative oxidative stress and low grade inflammation in the normal ageing process, independently of disease. therefore, arguably the best route with which to address premature ageing, as well as age-associated diseases such as diabetes, cardiovascular disease, and dementia, is preventative ...201627034739
quantitative trait loci affecting pathogen resistance and ripening of grapevines.grapevines (vitis vinifera l.) form the basis of viticulture, and are susceptible to diseases such as downy mildew (plasmopara viticola) and powdery mildew (erysiphe necator). therefore, successful viticulture programs require the use of pesticides. breeding for resistance is the only eco-friendly solution. marker-assisted selection is currently widely used for grapevine breeding. consequently, traits of interest must be tagged with molecular markers linked to quantitative trait loci (qtl). we h ...201627038830
household food items toxic to dogs and cats.several foods that are perfectly suitable for human consumption can be toxic to dogs and cats. food-associated poisoning cases involving the accidental ingestion of chocolate and chocolate-based products, allium spp. (onion, garlic, leek, and chives), macadamia nuts, vitis vinifera fruits (grapes, raisins, sultanas, and currants), products sweetened with xylitol, alcoholic beverages, and unbaked bread dough have been reported worldwide in the last decade. the poisoning episodes are generally due ...201627047944
structural and functional analysis of the gras gene family in grapevine indicates a role of gras proteins in the control of development and stress responses.gras transcription factors are involved in many processes of plant growth and development (e.g., axillary shoot meristem formation, root radial patterning, nodule morphogenesis, arbuscular development) as well as in plant disease resistance and abiotic stress responses. however, little information is available concerning this gene family in grapevine (vitis vinifera l.), an economically important woody crop. we performed a model curation of gras genes identified in the latest genome annotation l ...201627065316
grapevine pathogenic microorganisms: understanding infection strategies and host response scenarios.grapevine (vitis vinifera l.) is one of the most important fruit crop worldwide. commercial cultivars are greatly affected by a large number of pathogenic microorganisms that cause diseases during pre- and/or post-harvest periods, affecting production, processing and export, along with fruit quality. among the potential threats, we can find bacteria, fungi, oomycete, or viruses with different life cycles, infection mechanisms and evasion strategies. while plant-pathogen interactions are cycles o ...201627066032
advances in table grape breeding in japan.in japan, few grape cultivars related to vitis vinifera existed 200 years ago, on account of japan's high rainfall. many v. labruscana and vinifera cultivars were introduced to japan in the 19th century. labruscana was grown instead of vinifera, mainly because of severe disease problems and a high incidence of berry cracking. grape breeding for table use started in the 20th century, with the goal of combining the berry quality of vinifera with the ease of cultivation of labruscana. by 1945, thre ...201627069389
hybridization of cultivated vitis vinifera with wild v. californica and v. girdiana in california.hybridization of introduced domesticates and closely related natives is well documented in annual crops. the widespread introduction of the domesticated grapevine, vitis vinifera, into california where it overlaps with two native congenerics, with which it is interfertile, provides opportunity to investigate hybridization between woody perennials. although geographically widespread, the introduction over the past two centuries has been limited to a few elite clonal cultivars, providing a unique ...201527069616
odorant screening and quantitation of thiols in carmenere red wine by gas chromatography-olfactometry and stable isotope dilution assays.the sensory impact of thiols in vitis vinifera 'carmenere' red wines was evaluated. for this purpose, aroma extract dilution analysis was applied to the thiols isolated from a carmenere red wine by affinity chromatography with a mercurated agarose gel. results revealed the presence of four odorants, identified as 2-furanylmethanethiol, 3-sulfanylhexyl acetate, 3-sulfanyl-1-hexanol, and 2-methyl-3-sulfanyl-1-butanol, with the latter being described here for the first time in carmenere red wines. ...201627070203
the amino acid distribution in rachis xylem sap and phloem exudate of vitis vinifera 'cabernet sauvignon' bunches.amino acids are essential to grape berry and seed development and they are transferred to the reproductive structures through the phloem and xylem from various locations within the plant. the diurnal and seasonal dynamics of xylem and phloem amino acid composition in the leaf petiole and bunch rachis of field-grown cabernet sauvignon are described to better understand the critical periods for amino acid import into the berry. xylem sap was extracted by the centrifugation of excised leaf petioles ...201627082989
aba-mediated responses to water deficit separate grapevine genotypes by their genetic background.aba-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. however in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. to elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine vitis genotypes differing in their drought adaptation in the field. the transcript abundance of 12 genes involved in aba biosyn ...201627091220
clustering reveals cavitation-related acoustic emission signals from dehydrating branches.the formation of air emboli in the xylem during drought is one of the key processes leading to plant mortality due to loss in hydraulic conductivity, and strongly fuels the interest in quantifying vulnerability to cavitation. the acoustic emission (ae) technique can be used to measure hydraulic conductivity losses and construct vulnerability curves. for years, it has been believed that all the ae signals are produced by the formation of gas emboli in the xylem sap under tension. more recent expe ...201627095256
viticultural and chemical characteristics of muscat hamburg preselected clones grown for table grapes.clonal selection is one of the tools used for grapevine improvement and therefore is very important for obtaining clones with better characteristics than the variety population. the aim of this study was to select superior grapevines of vitis vinifera l. cv. muscat hamburg grown for fresh consumption.201727098241
identification of genomic sites for crispr/cas9-based genome editing in the vitis vinifera genome.crispr/cas9 has been recently demonstrated as an effective and popular genome editing tool for modifying genomes of humans, animals, microorganisms, and plants. success of such genome editing is highly dependent on the availability of suitable target sites in the genomes to be edited. many specific target sites for crispr/cas9 have been computationally identified for several annual model and crop species, but such sites have not been reported for perennial, woody fruit species. in this study, we ...201627098585
erratum to: geochemical characterization of elements in vitis vinifera cv. negroamaro grape berries grown under different soil managements. 201627106207
phylogeny of geminivirus coat protein sequences and digital pcr aid in identifying spissistilus festinus as a vector of grapevine red blotch-associated virus.grapevine red blotch-associated virus (grbav) is a single-stranded dna virus, proposed to be a member of the family geminiviridae, and is associated with grapevines showing red blotch symptoms in north america. the existence of the virus was reported in 2012, and subsequently detected in grapevines in major grape production regions. we investigated if a vector exists that can transmit grbav in vineyards. phylogenetic analysis of the predicted amino acid sequence of coat protein (cp) of grbav wit ...201627111804
[natural inducing factors of grape bud dormancy and their regulation on respiratory metabolism during dormancy induction].high chilling requirement grape (vitis vinifera-v. labrusca cv. summer black) was used to evaluate its dormancy under short sunlight day (sd), long sunlight day (ld) and natural condition (ck). the results indicated that grape bud dormancy could be induced by natural low temperature and short sunlight alone or together. short sunlight was the main contributor to the dormancy of grape bud, followed by natural low temperature. sd had more effect on dormancy induction under the same temperature whe ...201527112009
surface disinfection procedure and in vitro regeneration of grapevine (vitis vinifera l.) axillary buds.establishment of an efficient explants surface disinfection protocol is essential for in vitro cell and tissue culture as well as germplasm conservation, such as the case of grapevine (vitis spp.) culture. in this research, different procedures for disinfection and regeneration of field-grown grapevine cv. 'flame seedless' axillary buds were evaluated. the buds were disinfected using either naocl or allyl, benzyl, phenyl and 2-phenylethyl isothiocyanates. two different media for shooting and fou ...201627119057
the impact of canopy managements on grape and wine composition of cv. 'istrian malvasia' (vitis vinifera l.).the interest in producing wines preferred by consumers increases the need for improving practices to modify grape and wine composition. the aim of this study was to assess the impacts of three different canopy management measures, (1) early leaf removal in the cluster zone, (2) removal of young leaves above the second pair of wires and (3) double maturation raisonnée, on the yield and chemical composition of 'istrian malvasia' grape and wine.201627129430
isolation and purification of two antioxidant isomers of resveratrol dimer from the wine grape by counter-current chromatography.resveratrol dimers belong to a group of compounds called stilbenes, which along with proanthocyanidins, anthocyanins, catechins, and flavonols are natural phenolic compounds found in grapes and red wine. stilbenes have a variety of structural isomers, all of which exhibit various biological properties. counter-current chromatography with a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (2:5:4:5, v/v/v/v) was applied to isolate and purify stilbene from the stems of win ...201627130423
from grape to wine: changes in phenolic composition and its influence on antioxidant activity.the evolution of phenolic compounds and their relationship with the antioxidant capacity (ac) of samples taken along the winemaking process of three vitis vinifera l. cv., syrah, merlot and cabernet sauvignon grown in argentina were studied. forty-five compounds were identified by hplc-pda-ms/ms, while the ac was determined by frap, abts and dpph assays. results show that phenolic composition and ac vary along the winemaking process and between varieties. multiple regression analysis showed a hi ...201627132844
another look at the phylogenetic position of the grape order vitales: chloroplast phylogenomics with an expanded sampling of key lineages.vitales is well-known for including the economically important fruit crop, the wine grape (vitis vinifera). however, the position of the vitales in the higher eudicots has been a subject of much debate. it has been variously reported to be sister to the saxifragales and together as sister to the rest of rosids, or sister to the fabids-malvids clade, or sister to the santalales, or sister to the fabids-malvids-saxifragales clade. however, in all of these scenarios, the support values for the rela ...201627138293
non-water miscible ionic liquid improves biocatalytic production of geranyl glucoside with escherichia coli overexpressing a glucosyltransferase.whole cells of escherichia coli overexpressing a glucosyltransferase from vitis vinifera were used for the glucosylation of geraniol to geranyl glucoside. a high cell density cultivation process for the production of whole-cell biocatalysts was developed, gaining a dry cell mass concentration of up to 67.6 ± 1.2 g l(-1) and a glucosyltransferase concentration of up to 2.7 ± 0.1 g protein l(-1) within a process time of 48 h. whole-cell batch biotransformations in milliliter-scale stirred-tank bio ...201627142377
interactive effects of grapevine leafroll-associated virus 3 (glrav-3) and water stress on the physiology of vitis vinifera l. cv. malvasia de banyalbufar and giro-ros.among several biotic and abiotic stress combinations, interaction between drought and pathogen is one of the most studied combinations in some crops but still not in grapevine. in the present work, we focused on the interaction effects of biotic (glrav-3) and abiotic (drought) stresses on grapevine photosynthetic metabolism on two cultivars (cvs. 'malvasia de banyalbufar and giro-ros'). non-infected and glrav-3 infected potted plants were compared under water stress conditions (ws) and well-wate ...201627153513
chloroplast based genetic diversity among chinese grapes genotypes.chloroplast simple sequence repeat (cpssr) markers previously reported were used to analyze chloroplast diversity of chinese grapes. nine cpssr primer pairs from the chloroplast genomes of citrus and tobacco (nicotiana tabacum l.) were found useful for analyzing the grapes chloroplast genome (cpdna). selected cpssr primer pairs showed polymorphism among 64 varieties, with polymorphism information content (pic) values ranging from 0.522 to 0.659, with an average of 0.590. results depicted that a ...201727159719
the accumulation and localization of chalcone synthase in grapevine (vitis vinifera l.).chalcone synthase (chs, e.c.2.3.1.74) is the first committed enzyme in the flavonoid pathway. previous studies have primarily focused on the cloning, expression and regulation of the gene at the transcriptional level. little is yet known about the enzyme accumulation, regulation at protein level, as well as its localization in grapevine. in present study, the accumulation, tissue and subcellular localization of chs in different grapevine tissues (vitis vinifera l. cabernet sauvignon) were invest ...201627161583
molecular cloning and expression analyses of rps3a gene from mulberry under abiotic stresses and among different mulberry varieties.a full-length cdna sequence coding ribosomal protein s3a of mulberry tree, which we designated mmrps3a (genbank accession no. kr610331), was cloned based on mulberry expressed sequence tags. sequence analysis showed that the mmrps3a is 1089 bp long and contains a 80-bp 5'-utr (untranslated region) and a 220-bp 3'-utr. its open reading frame consists of a 789-bp encoding 262 amino acids with a predicted molecular weight of 30.053 kda and an isoelectric point of 9.84. homology analysis revealed th ...201627173298
image analysis-based modelling for flower number estimation in grapevine.grapevine flower number per inflorescence provides valuable information that can be used for assessing yield. considerable research has been conducted at developing a technological tool, based on image analysis and predictive modelling. however, the behaviour of variety-independent predictive models and yield prediction capabilities on a wide set of varieties has never been evaluated.201727173452
impact of leaf removal, applied before and after flowering, on anthocyanin, tannin, and methoxypyrazine concentrations in 'merlot' (vitis vinifera l.) grapes and wines.the development and accumulation of secondary metabolites in grapes determine wine color, taste, and aroma. this study aimed to investigate the effect of leaf removal before flowering, a practice recently introduced to reduce cluster compactness and botrytis rot, on anthocyanin, tannin, and methoxypyrazine concentrations in 'merlot' grapes and wines. leaf removal before flowering was compared with leaf removal after flowering and an untreated control. no effects on tannin and anthocyanin concent ...201627180819
de novo synthesis of benzenoid compounds by the yeast hanseniaspora vineae increases the flavor diversity of wines.benzyl alcohol and other benzenoid-derived metabolites of particular importance in plants confer floral and fruity flavors to wines. among the volatile aroma components in vitis vinifera grape varieties, benzyl alcohol is present in its free and glycosylated forms. these compounds are considered to originate from grapes only and not from fermentative processes. we have found increased levels of benzyl alcohol in red tannat wine compared to that in grape juice, suggesting de novo formation of thi ...201627193819
novel small molecule 11β-hsd1 inhibitor from the endophytic fungus penicillium commune.two new phenone derivatives penicophenones a (1) and b (2), a new cyclic tetrapeptide penicopeptide a (3), and five known compounds were isolated from the culture broth of penicillium commune, an endophytic fungus derived from vitis vinifera. compounds 1-3 were elucidated by extensive spectroscopic analyses including 1d and 2d nmr and hresims. the absolute configurations of 1 and 3 were determined by comparing its ecd with related molecules and modified marfey's analysis, respectively. penicophe ...201627194583
review of the pharmacological effects of vitis vinifera (grape) and its bioactive constituents: an update.vitis vinifera fruit (grape) contains various phenolic compounds, flavonoids and stilbenes. in recent years, active constituents found in the fruits, seeds, stems, skin and pomaces of grapes have been identified and some have been studied. in this review, we summarize the active constituents of different parts of v. vinifera and their pharmacological effects including skin protection, antioxidant, antibacterial, anticancer, antiinflammatory and antidiabetic activities, as well as hepatoprotectiv ...027196869
aroma profile of montepulciano d'abruzzo wine fermented by single and co-culture starters of autochthonous saccharomyces and non-saccharomyces yeasts.montepulciano d'abruzzo is a native grape variety of vitis vinifera l., grown in central italy and used for production of high quality red wines. limited studies have been carried out to improve its enological characteristics through the use of indigenous strains of saccharomyces cerevisiae. the main objective of the present work was to test two indigenous strains of s. cerevisiae (srs1, rt73), a strain of starmerella bacillaris (sts12), one of hanseniaspora uvarum (sts45) and a co-culture of s. ...201627199939
linking jasmonic acid to grapevine resistance against the biotrophic oomycete plasmopara viticola.plant resistance to biotrophic pathogens is classically believed to be mediated through salicylic acid (sa) signaling leading to hypersensitive response followed by the establishment of systemic acquired resistance. jasmonic acid (ja) signaling has extensively been associated to the defense against necrotrophic pathogens and insects inducing the accumulation of secondary metabolites and pr proteins. moreover, it is believed that plants infected with biotrophic fungi suppress ja-mediated response ...201627200038
collagen-collagen interactions mediated by plant-derived proanthocyanidins: a spectroscopic and atomic force microscopy study.collagen cross-linkings are determinant of biological tissue stability and function. plant-derived proanthocyanidins (pacs) mimic different hierarchical levels of collagen cross-links by non-enzymatic interactions resulting in the enhancement to the biomechanics and biostability of collagen-rich tissues such as dentin. this study investigated the interaction of pacs from vitis vinifera grape seed extract with type i collagen in solubilized form and in the demineralized dentin matrix (ddm) by flu ...201627208639
examination of correlation between histidine and cadmium absorption by eleagnus angustifolia l., vitis vinifera l. and nerium oleander l. using hplc-ms and icp-ms.in this study, hplc-ms and icp-ms methods wereused for the determination of histidine and cadmium in eleagnus angustifolia l., vitis vinifera l. and nerium oleander l. leaves taken from industrial area including gaziantep and bursa cities. to histidine determination by hplc-ms, flow rate of mobile phase, fragmentor potential, injection volume and column temperature were optimized as 0.2 ml · min⁻¹, 70 v, 15 µl and 20 °c, respectively. for extraction of histidine from plants, distilled water was ...201627209774
the use of glucose oxidase and catalase for the enzymatic reduction of the potential ethanol content in wine.due to the increase of sugar levels in wine grapes as one of the impacts of climate change, alcohol reduction in wines becomes a major focus of interest. this study combines the use of glucose oxidase and catalase activities with the aim of rapid conversion of glucose into non-fermentable gluconic acid. the h2o2 hydrolysing activity of purified catalase is necessary in order to stabilize glucose oxidase activity. after establishing the adequate enzyme ratio, the procedure was applied in large-sc ...201627211694
identification of a vitis vinifera endo-β-1,3-glucanase with antimicrobial activity against plasmopara viticola.inducible plant defences against pathogens are stimulated by infections and comprise several classes of pathogenesis-related (pr) proteins. endo-β-1,3-glucanases (egases) belong to the pr-2 class and their expression is induced by many pathogenic fungi and oomycetes, suggesting that egases play a role in the hydrolysis of pathogen cell walls. however, reports of a direct effect of egases on cell walls of plant pathogens are scarce. here, we characterized three egases from vitis vinifera whose ex ...201627216084
rain-shelter cultivation modifies carbon allocation in the polyphenolic and volatile metabolism of vitis vinifera l. chardonnay grapes.this study investigated the effect of rain-shelter cultivation on the biosynthesis of flavonoids and volatiles in grapes, with an aim of determining whether rain-shelter application could help to improve the sensory attributes and quality of grapes. vitis vinifera l. chardonnay grapes, grown in the huaizhuo basin region of northern china, were selected within two consecutive years. a rain-shelter roof was constructed using a colorless polyethylene (pe) film with a light transmittance of 80%. res ...201627218245
non-polyphenolic compounds of a specific kind of dried grape (maviz) inhibit memory impairments induced by beta-amyloid peptide.although grape has been recently the topic of many investigations, maviz (a kind of dried one) has remained neglected. the aim of this study was to assess anti-alzheimer activity of maviz.201727219682
[effects of rootstocks on the growth and berry quality of vitis vinifera cv. cabernet sauvignon grapevine in changli zone, hebei province, china].cabernet sauvignon grafted onto seven rootstocks 188-08, 5bb, so4, 3309c, 110r, 5c and 101-14m, with the own-rooted vines as control, were investigated to study the effects of different rootstocks on the growth, fruit quality and yield of cabernet sauvignon in changli zone, hebei province, china. the results showed that cabernet sauvignon grafted on 5bb and 5c significantly increased the trunk diameter, and 5c significantly increased one-year-old shoot diameter. 188-08, 5bb and 5c as rootstock o ...201627228593
rhizosphere associated bacteria trigger accumulation of terpenes in leaves of vitis vinifera l. cv. malbec that protect cells against reactive oxygen species.it has been proposed that plant growth promoting rhizobacteria (pgpr) stimulate plant growth and development by inducing the biosynthesis of secondary metabolites, like terpenes, which reduce stress incidence. three bacteria previously isolated from grapevine roots and adjacent soil (microbacterium imperiale rz19m10, kocuria erythromyxa rt5m10 and terribacillus saccharophilus rt17m10) were tested as pgpr. after 30 days since root inoculation of in vitro grown vitis vinifera cv. malbec plants, th ...201627231874
proteome of plasmopara viticola-infected vitis vinifera provides insights into grapevine rpv1/rpv3 pyramided resistance to downy mildew.grapevine is one of the major fruit crops worldwide and requires phytochemical use due to susceptibility to numerous pests, including downy mildew. the pyramiding of previous identified qtl resistance regions allows selection of genotypes with combined resistance loci in order to build up sustainable resistance. this study investigates resistance response of pyramided plants containing rpv1 and rpv3 loci to plasmopara viticola infection process. phenotypic characterization showed complete resist ...201727235723
quantitative real-time pcr normalization for gene expression studies in the plant pathogenic fungi lasiodiplodia theobromae.lasiodiplodia theobromae is a highly virulent plant pathogen. it has been suggested that heat stress increases its virulence. the aim of this work was to evaluate, compare, and recommend normalization strategies for gene expression analysis of the fungus growing with grapevine wood under heat stress. using rt-qpcr-derived data, reference gene stability was evaluated through genorm, normfinder and bestkeeper applications. based on the geometric mean using the ranking position obtained for each in ...201627237774
phenology, canopy aging and seasonal carbon balance as related to delayed winter pruning of vitis vinifera l. cv. sangiovese grapevines.manipulating or shifting annual grapevine growing cycle to offset limitations imposed by global warming is a must today, and delayed winter pruning is a tool to achieve it. however, no information is available about its physiological background, especially in relation to modifications in canopy phenology, demography and seasonal carbon budget. mechanistic hypothesis underlying this work was that very late winter pruning (lwp) can achieve significant postponement of phenological stages so that ri ...201627242860
metabolic consequences of infection of grapevine (vitis vinifera l.) cv. "modra frankinja" with flavescence dorée phytoplasma.flavescence dorée, caused by the quarantine phytoplasma fdp, represents the most devastating of the grapevine yellows diseases in europe. in an integrated study we have explored the fdp-grapevine interaction in infected grapevines of cv. "modra frankinja" under natural conditions in the vineyard. in fdp-infected leaf vein-enriched tissues, the seasonal transcriptional profiles of 14 genes selected from various metabolic pathways showed an fdp-specific plant response compared to other grapevine y ...201627242887
combined physiological, transcriptome, and cis-regulatory element analyses indicate that key aspects of ripening, metabolism, and transcriptional program in grapes (vitis vinifera l.) are differentially modulated accordingly to fruit size.in wine grape production, management practices have been adopted to optimize grape and wine quality attributes by producing, or screening for, berries of smaller size. fruit size and composition are influenced by numerous factors that include both internal (e.g. berry hormone metabolism) and external (e.g. environment and cultural practices) factors. combined physiological, biochemical, and transcriptome analyses were performed to improve our current understanding of metabolic and transcriptiona ...201627245662
adaptation of a plant pathogen to partial host resistance: selection for greater aggressiveness in grapevine downy mildew.an understanding of the evolution of pathogen quantitative traits in response to host selective pressures is essential for the development of durable management strategies for resistant crops. however, we still lack experimental data on the effects of partial host resistance on multiple phenotypic traits (aggressiveness) and evolutionary strategies in pathogens. we performed a cross-inoculation experiment with four grapevine hosts and 103 isolates of grapevine downy mildew (plasmopara viticola) ...201627247621
protective effect of aqueous seed extract of vitis vinifera against oxidative stress, inflammation and apoptosis in the pancreas of adult male rats with diabetes mellitus.protective effects of vitis vinifera seed aqueous extract (vvsae) against pancreatic dysfunctions and elevation of oxidative stress, inflammation and apoptosis in the pancreas in diabetes were investigated. histopathological changes in the pancreas were examined under light microscope.201627261624
functional proteomic analysis of ankaferd® blood stopper.ankaferd® blood stopper (abs) comprises a standardized mixture of the plants thymus vulgaris, glycyrrhiza glabra, vitis vinifera, alpinia officinarum, and urtica dioica. the basic mechanism of action for abs is the formation of an encapsulated protein network that provides focal points for vital erythrocyte aggregation. abs-induced protein network formation with blood cells, particularly erythrocytes, covers the primary and secondary hemostatic system without disturbing individual coagulation fa ...201027263447
daily preharvest uv-c light maintains the high stilbenoid concentration in grapes.the fact that it is possible to induce stilbenoid synthesis in grapevine (vitis vinifera) by uv-c light allows the possibility of stimulating grapevine phytoalexin production to increase disease resistance and immunity, and subsequently to limit the use of pesticides in vineyards. uv-c light was applied daily during three days before the harvesting of table grape variety crimson seedless to study the accumulation of stilbenoid compounds during ripeness. the e-resveratrol concentration was monito ...201627268503
rhizotubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation.in order to maintain high yields while saving water and preserving non-renewable resources and thus limiting the use of chemical fertilizer, it is crucial to select plants with more efficient root systems. this could be achieved through an optimization of both root architecture and root uptake ability and/or through the improvement of positive plant interactions with microorganisms in the rhizosphere. the development of devices suitable for high-throughput phenotyping of root structures remains ...201627279895
identification and quantification of phenolic compounds in berry skin, pulp, and seeds in 13 grapevine varieties grown in serbia.phenolic profile of 13 grapevine varieties was determined, with respect to three different parts of berries: skin, seed and pulp. identification and quantification of the phenols was done using ultra-high performance liquid chromatography (uhplc) coupled with a diode array detector and a triple-quadrupole mass spectrometer. the most abundant phenols in grape seeds were found to be flavan-3-ols, most of which are gallocatechin gallate and catechin. the skins were characterized mostly by flavonols ...201627283628
grape aroma precursors in cv. nebbiolo as affected by vine microclimate.the influence exerted by bunch microclimate on some c13-norisoprenoid precursors content was investigated for the first time in nebbiolo grapes during ripening. samples were collected, during two consecutive seasons, from two vineyards, which are characterized by different microclimatic conditions caused by vine vigour heterogeneity and different vineyard aspects. enzymatic hydrolysis of the glycosides extracted from the grapes, and subsequent gc-ms determination of the aglycones, highlighted th ...201627283716
scavenging and antioxidant properties of different grape cultivars against ionizing radiation-induced liver damage ex vivo.ionizing radiation (ir) has become an integral part of the modern medicine--both for diagnosis as well as therapy. however, normal tissues or even distant cells also suffer ir-induced free radical insult. it may be more damaging in longer term than direct radiation exposure. antioxidants provide protection against ir-induced damage. grapes are the richest source of antioxidants. here, we assessed the scavenging properties of four grape (vitis vinifera) cultivars, namely flame seedless (black), k ...201627295925
associations among wine grape microbiome, metabolome, and fermentation behavior suggest microbial contribution to regional wine characteristics.regionally distinct wine characteristics (terroir) are an important aspect of wine production and consumer appreciation. microbial activity is an integral part of wine production, and grape and wine microbiota present regionally defined patterns associated with vineyard and climatic conditions, but the degree to which these microbial patterns associate with the chemical composition of wine is unclear. through a longitudinal survey of over 200 commercial wine fermentations, we demonstrate that bo ...201627302757
the novel gene vppr4-1 from vitis pseudoreticulata increases powdery mildew resistance in transgenic vitis vinifera l.pathogenesis-related proteins (prs) can lead to increased resistance of the whole plant to pathogen attack. here, we isolate and characterize a pr-4 protein (vppr4-1) from a wild chinese grape vitis pseudoreticulata which shows greatly elevated transcription following powdery mildew infection. its expression profiles under a number of abiotic stresses were also investigated. powdery mildew, salicylic acid, and jasmonic acid methyl ester significantly increased the vppr4-1 induction while nacl an ...201627303413
responses of in vitro-grown plantlets (vitis vinifera) to grapevine leafroll-associated virus-3 and peg-induced drought stress.stresses caused by viral diseases and drought have long threatened sustainable production of grapevine. these two stresses frequently occur simultaneously in many of grapevine growing regions of the world. we studied responses of in vitro-grown plantlets (vitis vinifera) to grapevine leafroll associated virus-3 (glrav-3) and peg-induced drought stress. results showed that stress induced by either virus infection or drought had negative effects on vegetative growth, caused significant decreases a ...201627313542
correction to mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-o-diglucosides in cabernet sauvignon (vitis vinifera l.) grape berries. 201627322837
transmission of grapevine virus a and grapevine leafroll-associated virus 1 and 3 by heliococcus bohemicus (hemiptera: pseudococcidae) nymphs from plants with mixed infections.mealybugs (hemiptera: pseudococcidae) represent a serious threat for viticulture as vectors of phloem-restricted viruses associated with the grapevine rugose wood and leafroll diseases. heliococcus bohemicus (šulc) is known to be involved in the spread of these two viral diseases, being a vector of the grapevine virus a (gva) and the grapevine leafroll-associated virus 1 and 3 (glrav-1 and glrav-3). this study investigated the acquisition and transmission efficiency of h. bohemicus fed on mixed- ...201627329628
tartaric acid pathways in vitis vinifera l. (cv. ugni blanc): a comparative study of two vintages with contrasted climatic conditions.the acid component of grape berries, originating in the metabolism of malate and tartrate, the latter being less well-known than the former, is a key factor at play in the microbiological stability of wines destined for distillation. grape acidity is increasingly affected by climate changes. the ability to compare two vintages with contrasted climatic conditions may contribute to a global understanding of the regulation of acid metabolism and the future consequences for berry composition.201627350040
response of vitis vinifera cell cultures to eutypa lata and trichoderma atroviride culture filtrates: expression of defence-related genes and phenotypes.cell suspension cultures of vitis vinifera cv. dauphine berries were used to study the response to the vascular pathogen, eutypa lata, in comparison with a biological control agent, trichoderma atroviride, that was previously shown to be effective in pruning wound protection. the expression of genes coding for enzymes of the phenylpropanoid pathway and pathogenesis-related (pr) proteins was profiled over a 48-h period using quantitative reverse transcriptase pcr. the cell cultures responded to e ...201727352313
genomic ancestry estimation quantifies use of wild species in grape breeding.grapes are one of the world's most valuable crops and most are made into wine. grapes belong to the genus vitis, which includes over 60 inter-fertile species. the most common grape cultivars derive their entire ancestry from the species vitis vinifera, but wild relatives have also been exploited to create hybrid cultivars, often with increased disease resistance.201627357509
single vessel air injection estimates of xylem resistance to cavitation are affected by vessel network characteristics and sample length.xylem resistance to cavitation is an important trait that is related to the ecology and survival of plant species. vessel network characteristics, such as vessel length and connectivity, could affect the spread of emboli from gas-filled vessels to functional ones, triggering their cavitation. we hypothesized that the cavitation resistance of xylem vessels is randomly distributed throughout the vessel network. we predicted that single vessel air injection (svai) vulnerability curves (vcs) would t ...201627358206
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