Publications

elevated co2 levels increase the toxicity of zno nanoparticles to goldfish (carassius auratus) in a water-sediment ecosystem.	concerns about the environmental safety of metal-based nanoparticles (mnps) in aquatic ecosystems are increasing. simultaneously, elevated atmospheric co2 levels are a serious problem worldwide, making it possible for the combined exposure of mnps and elevated co2 to the ecosystem. here we studied the toxicity of nzno to goldfish in a water-sediment ecosystem using open-top chambers flushed with ambient (400±10μl/l) or elevated (600±10μl/l) co2 for 30days. we measured the content of zn in suspen ...	2017	28040633
ghrelin modulates gene and protein expression of digestive enzymes in the intestine and hepatopancreas of goldfish (carassius auratus) via the ghs-r1a: possible roles of plc/pkc and ac/pka intracellular signaling pathways.	ghrelin, a multifunctional gut-brain hormone, is involved in the regulation of gastric functions in mammals. this study aimed to determine whether ghrelin modulates digestive enzymes in goldfish (carassius auratus). immunofluorescence microscopy found colocalization of ghrelin, ghs-r1a and the digestive enzymes sucrase-isomaltase, aminopeptidase a, trypsin and lipoprotein lipase in intestinal and hepatopancreatic cells. in vitro ghrelin treatment in intestinal and hepatopancreas explant culture ...	2017	28042022
occurrence of parasites of the genus eustrongylides spp. (nematoda: dioctophymatidae) in fish caught in trasimeno lake, italy.	eustrongylides spp. is considered a freshwater fish zoonotic nematode. in the present study, the prevalence of eustrongylides spp. in six edible fish (european perch - perca fluviatilis, goldfish - carassius auratus, largemouth black bass - micropterus salmoides, tench- tinca tinca, carp - cyprinus carpio and sand smelt - atherina boyeri) of trasimeno lake was surveyed. the investigations were conducted from october 2014 to september 2015 and 384 specimens per species for each season were caught ...	2016	28058245
glucose, amino acids and fatty acids directly regulate ghrelin and nucb2/nesfatin-1 in the intestine and hepatopancreas of goldfish (carassius auratus) in vitro.	ghrelin and nesfatin-1 are two peptidyl hormones primarily involved in food intake regulation. we previously reported that the amount of dietary carbohydrates, protein and lipids modulates the expression of these peptides in goldfish in vivo. in the present work, we aimed to characterize the effects of single nutrients on ghrelin and nesfatin-1 in the intestine and hepatopancreas. first, immunolocalization of ghrelin and nucb2/nesfatin-1 in goldfish hepatopancreas cells was studied by immunohist ...	2017	28089858
establishment and characterization of a heart-derived cell line from goldfish (carassius auratus).	the goldfish carassius auratus, a freshwater fish in the family cyprinidae, was one of the earliest fish to be domesticated for ornamental purposes. a cell line was established from goldfish heart (gh) tissue to create a biological monitoring tool for viral diseases. the gh cell line was optimally maintained at 25 °c in m199 medium supplemented with 10-20% fetal bovine serum. a chromosomal analysis indicated that the cell line remained diploid, with a mean chromosomal count of 100. in viral inoc ...	2017	28097494
composition of gut microbiota in the gibel carp (carassius auratus gibelio) varies with host development.	to understand how a bacteria-free fish gut ecosystem develops microbiota as the fish ages, we performed a 1-year study on the gut microbiota of hatchling gibel carp (carassius auratus gibelio). our results indicate that the gut microbial diversity increases significantly as the fish develop. the gut microbial community composition showed significant shifts corresponding to host age and appeared to shift at two time points despite consistent diet and environmental conditions, suggesting that some ...	2017	28108758
proteome response of fish under multiple stress exposure: effects of pesticide mixtures and temperature increase.	aquatic systems can be subjected to multiple stressors, including pollutant cocktails and elevated temperature. evaluating the combined effects of these stressors on organisms is a great challenge in environmental sciences. to the best of our knowledge, this is the first study to assess the molecular stress response of an aquatic fish species subjected to individual and combined pesticide mixtures and increased temperatures. for that, goldfish (carassius auratus) were acclimated to two different ...	2017	28109940
diversity of culturable bacterial communities in the intestinal tracts of goldfish (carassius auratus) and their ability to produce n-acyl homoserine lactone.	intestinal bacteria isolated from goldfish (carassius auratus) were identified based on 16 ribosomal rna (rrna) gene sequences and screened for their ability to produce n-acyl homoserine lactone (ahl), an autoinducer of the quorum sensing (qs) system. the 230 aerobes/facultative anaerobes that were isolated comprised members of the genera aeromonas (184 isolates), citrobacter (11), enterobacter (2), shewanella (28), vagococcus (1), and vibrio (4). among these genera, the two most abundant specie ...	2017	28124783
ifna2 of triploid hybrid of gold fish and allotetraploid is an intracellular antiviral cytokine against svcv and gcrv.	sterile triploid hybrids (3n = 150) of gold fish (carassius auratus red var., ♀, 2n = 100) and allotetroploid (♂, 2n = 100) display obviously improved disease resistance and much enhanced growth rate than their parents, which have been cultured widely in china. in this paper, one of the type i ifns of triploid hybrid (3nifna2) has been cloned and characterized. the full-length cdna of 3nifna2 gene consists of 715 nucleotides and the predicted 3nifna2 contains 183 amino acids. the transcription o ...	2017	28126620
identification of the putative goldfish (carassius auratus) magnesium transporter slc41a1 and functional regulation in the gill, kidney, and intestine in response to dietary and environmental manipulations.	while magnesium requirements for teleost fish highlight the physiological importance of this cation for homeostasis, little is known regarding the molecular identity of transporters responsible for magnesium absorption or secretion. the recent characterization of the vertebrate magnesium transporter solute carrier 41a1 (slc41a1) in the kidney of a euryhaline fish has provided a glimpse of possible moieties involved in piscine magnesium regulation. the present study obtained a novel slc41a1 codin ...	2017	28130070
allometric analysis of a morphological anti-predator trait in geographic populations of japanese crucian carp.	costly anti-predator traits tend to be expressed only in high-predation conditions. for the cyprinid fish genus carassius, deeper body depth is more adaptive to avoid predation by gape-limited piscivorous fish, but it raises swimming costs. it is therefore predicted that the relative body depth will decrease when the prey fish has reached a size larger than the predator gape-size. this prediction was tested by allometric analysis of the relation between body depth and standard length of triploid ...	2017	28150742
characterization of ghrelin o-acyltransferase (goat) in goldfish (carassius auratus).	ghrelin is the only known hormone posttranslationally modified with an acylation. this modification is crucial for most of ghrelin's physiological effects and is catalyzed by the polytopic enzyme ghrelin o-acyltransferase (goat). the aim of this study was to characterize goat in a teleost model, goldfish (carassius auratus). first, the full-length cdna sequence was obtained by rt-pcr and rapid amplification of cdna ends methods. two highly homologous cdnas of 1491 and 1413 bp, respectively, name ...	2017	28178327
comparative pharmacokinetics of enrofloxacin in healthy and aeromonas hydrophila-infected crucian carp (carassius auratus gibelio).	the comparative pharmacokinetics of enrofloxacin (enr) and its metabolite ciprofloxacin (cip) were investigated in healthy and aeromonas hydrophila-infected crucian carp after a single oral (p.o.) administration at a dose of 10 mg/kg at 25 °c. the plasma concentrations of enr and of cip were determined by hplc. pharmacokinetic parameters were calculated based on mean enr concentrations by noncompartmental modeling. in healthy fish, the elimination half-life (t1/2λz ), maximum plasma concentratio ...	2017	28205238
microcystin bioaccumulation in freshwater fish at different trophic levels from the eutrophic lake chaohu, china.	the spatial and temporal variations of microcystins (mcs) in fishes with different trophic levels were studied monthly in lake chaohu in 2014. mcs content in muscle was highest in phytoplanktivorous hypophthalmichthys molitrix (h. molitrix), followed by omnivorous carassius auratus (c. auratus), and was lowest in herbivorous parabramis pekinensis (p. pekinensis) and carnivorous coilia ectenes (c. ectenes). mcs concentration in liver was highest in c. auratus, followed by h. molitrix, and was low ...	2017	28213674
irisin in goldfish (carassius auratus): effects of irisin injections on feeding behavior and expression of appetite regulators, uncoupling proteins and lipoprotein lipase, and fasting-induced changes in fndc5 expression.	irisin is a peptide cleaved from the fibronectin type iii domain containing protein 5 (fndc5) gene that is secreted predominantly by muscle cells but also by other tissues including brain and intestine. in mammals, irisin has been shown to have thermogenic actions via the modulation of uncoupling proteins (ucps) and to affect feeding and energy homeostasis via actions in brain, adipose tissue, liver, muscle and gastrointestinal tract. to examine the role of irisin on feeding and metabolism in fi ...	2017	28219696
effects on heavy metal accumulation in freshwater fishes: species, tissues, and sizes.	three fish species (carassius auratus, pelteobagrus fulvidraco, and squaliobarbus curriculus) were collected from xiang river near changsha city, southern china. the concentrations of heavy metals including arsenic (as), cadmium (cd), copper (cu), iron (fe), manganese (mn), lead (pb), and zinc (zn) in the muscle, gill, and liver of three species were determined by the inductively coupled plasma mass spectrometry method. one-way analysis of variance (anova) was introduced to determine the signifi ...	2017	28233209
opisthorchis viverrini infection in the snail and fish intermediate hosts in central vietnam.	opisthorchis viverrini, a carcinogenic fish borne fluke, requires freshwater snails and fish as intermediate hosts. opisthorchiasis is endemic in parts of southeast asia, including central and south vietnam. in this region the transmission by intermediate hosts has received little attention. therefore, freshwater snails and wild fish from bau my tho, an opisthorchiasis endemic area in binh dinh province were collected for examination of o. viverrini cercariae and metacercariae, respectively. a t ...	2017	28242064
diverse chlamydia-like agents associated with epitheliocystis infection in two cyprinid fish species, the common carp (cyprinus carpio l.) and the gibel carp (carassius auratus gibelio l.).	during a general annual fish health survey in natural waters and ponds, epitheliocystis infections were recorded in fingerlings of two cyprinid fish species, the cultured common carp and the wild gibel carp. benign and heavy infections were equally observed without mortality. in addition to the general health inspection of fish, histopathological examinations of infected gills and molecular biological investigations of separated epitheliocysts were performed. epitheliocysts were formed both in t ...	2017	28244339
molecular characterization and expression analysis of toll-like receptors 5 and 22 from natural triploid carassius auratus.	innate immunity, as the most primitive and universal host defense in fish, constitutes an efficient first line of defense to combat invading microbes. toll-like receptors (tlrs) play essential roles in the innate immunity, and tlr5 and tlr22 are two important tlrs that can recognize flagellin and double stranded rna (dsrna), respectively. in this study, we identified and characterized two tlrs genes of qihe crucian carp (carassius auratus) (designated as catlr5 and catlr22). the full-length cdna ...	2017	28259778
molecular phylogenetics of centrocestus formosanus (digenea: heterophyidae) originated from freshwater fish from chiang mai province, thailand.	this study aimed to investigate the morphology and reconstruct the phylogenetic relationships of centrocestus formosanus originating from 5 species of freshwater fish, i.e., esomus metallicus, puntius brevis, anabas testudineus, parambassis siamensis, and carassius auratus, in chiang mai province, thailand. sequence-related amplified polymorphism (srap) and phylogeny based on internal transcribed spacer 2 (its2) and mitochondrial cytochrome c oxidase subunit 1 (co1) were performed. the results s ...	2017	28285504
vegetalising factor extracted from the fish swimbladder and tested on presumptive ectoderm oftriturus embryos.	vegetalising factor was isolated from swimbladder of crusian carp (carassius auratus) by solubilishing with 8 m urea the precipitate obtained after digesting the swimbladder with collagenase. the urea-soluble fraction vegetalised isolated presumptive ectoderm oftriturus gastrula and produced both undifferentiated mesodermal and endodermal cells. brief heating of the fraction changed its capacity to produce organised mesodermal tissues, such as notochord and somite, and the frequency of induction ...	1977	28304885
first report of rahnella aquatilis infection in crucian carp carassius auratus in china.	rahnella aquatilis infection is rare in aquaculture. here, a gram-negative rod-shaped bacterium was isolated from diseased crucian carp carassius auratus in xuzhou city, jiangsu province, eastern china. the isolate was tentatively named strain kcl-5, and subsequently identified as r. aquatilis by biochemical properties and molecular techniques. the results showed that the isolate kcl-5 was most closely related to the type strain atcc33071 (= dsm4594) of r. aquatilis, which shared 99.67, 96.26 an ...	2017	28322207
influence of multi-walled carbon nanotubes on the effects of roxithromycin in crucian carp (carassius auratus) in the presence of natural organic matter.	carbon nanotubes are increasingly entering the aquatic environment and may interact with other co-existing contaminants, such as antibiotics. however, whether these interactions may affect their bioavailability in aquatic organisms is the subject of considerable debate. the primary objective of this study was to assess the risks arising from the coexistence of roxithromycin (rox) and multi-walled carbon nanotubes (mwcnts) in waters containing natural organic matter (nom), focusing on the distrib ...	2017	28324838
ghrelin facilitates glut2-, sglt1- and sglt2-mediated intestinal glucose transport in goldfish (carassius auratus).	glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. this study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (carassius auratus). three intestinal glucose transporters, the facilitative glucose transporter 2 (glut2), and the sodium/glucose co-transporters 1 (sglt1) and 2 (sglt2), were studied. immunostaining of intestinal sections found colocalization of ghrelin and ...	2017	28338019
bioconcentration and multi-biomarkers of organic uv filters (bm-dbm and od-paba) in crucian carp.	organic uv filters (ouv-fs) are increasingly used in sunscreens and personal care products. in the present work, the bioconcentration and multi-biomarker effects of butyl methoxydibenzoylmethane (bm-dbm) and ethylhexyl dimethyl p-aminobenzoate (od-paba) were investigated in crucian carp (carassius auratus). the fish were exposed to various concentrations of bm-dbm (3.88, 35.61, 181.85 and 337.15μg/l), od-paba (4.66, 53.83, 264.22 and 459.32μg/l) and their mixture (2.31+2.79, 23.69+26.18, 97.37+1 ...	2017	28343007
development of a multiplex pcr method for the simultaneous detection of four myxosporeans infecting gibel carp carassius auratus gibelio.	gibel carp carassius auratus gibelio (bloch), a commercially important freshwater-cultured fish in china, is threatened by myxosporeans, particularly thelohanellus wuhanensis, myxobolus honghuensis, m. wulii and m. turpisrotundus. here, we developed a multiplex pcr assay for simultaneous detection of these 4 myxosporeans. the specific primers for each species were designed based on the 28s rdna gene of t. wuhanensis, the its-5.8s rdna of m. honghuensis and m. wulii, and the 18s rdna gene of m. t ...	2017	28357984
propofol as an immersion anesthetic and in a minimum anesthetic concentration (mac) reduction model in goldfish (carassius auratus).	propofol is a novel immersion anesthetic in goldfish ( carassius auratus ). objectives were to characterize propofol as an anesthetic and assess its suitability in a minimum anesthetic concentration (mac) reduction model. using a crossover design, eight goldfish were submerged in 1, 5, or 10 mg/l propofol. data included induction time, recovery time, heart rate, opercular rate, and response to supramaximal stimulation. baseline mac (dixon's up-and-down method) was determined, and 15 fish were an ...	2017	28363074
bacterial signatures of "red-operculum" disease in the gut of crucian carp (carassius auratus).	fish gut microbiota play important roles in fish immunity, nutrition, and the adaptation to environmental changes. to date, few studies have focused on the interactions among environmental factors, fish diseases, and gut microbiota compositions. we compared the gut bacterial communities of healthy crucian carps (carassius auratus) with those of individuals affected by "red-operculum" disease and corresponding water and sediment microbiota in four fish farm ponds. distinct gut bacterial communiti ...	2017	28364130
the interactive effect of digesting a meal and thermal acclimation on maximal enzyme activities in the gill, kidney, and intestine of goldfish (carassius auratus).	surrounding environmental temperatures affect many aspects of ectotherm physiology. generally, organisms can compensate at one or more biological levels, or allow temperature to dictate processes such as enzyme activities through kinetic effects on reaction rates. as digestion also alters physiological processes such as enzyme activities, this study determined the interacting effect of thermal acclimation (8 and 20 °c) and digesting a single meal on maximal enzyme activities in three tissues of ...	2017	28382530
stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish.	intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ...	2017	28389237
plasma pharmacokinetics and muscle residue dynamics of mebendazole in carassius auratus.	mebendazole is approved for use in aquatic animals and is widely used in chinese aquaculture. we developed a pharmacokinetic and residue analysis for mebendazole levels in the goldfish (carassius auratus). plasma and muscle samples of c. auratus were taken after oral administration of 10 mg/kg mebendazole. the maximal drug plasma concentration of 0.55 mg/l was achieved at 48 hr and then declined with the elimination half-life (t1/2β ) of 7.99 hr. administration of 10 mg/kg by oral gavage for 5 s ...	2017	28401566