Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [frequency of trichomonas vaginalis infection in apparently healthy women in western santiago]. | the incidence of infection by trichomonas vaginalis in apparently healthy women was surveyed in the western area of santiago, chile, which comprised both urban and rural areas with medium- or low-income people. a retrospective review of the data of the cytopathology laboratory of san juan de dios hospital was conducted. the data contained all annual pap smears and epidemiological information about the presenting patients. during 1983, a total of 29,501 women had pap smears taken to detect sig ... | 1985 | 12320978 |
| [oral contraceptive use and prevalence of infection with chlamydia trachomatis in women (author's transl)]. | 1080 nonpregnant women ages 16-34 years, presenting for the 1st time at a clinic for sexually transmitted diseases (std), were examined and screened for infection with chlamydia trachomatis, neisseria gonorrheae, trichomonas vaginalis, and candida species. the respective prevalence rates were 21.1%, 20.7%, 13.4%, and 27.8%. isolation rates for chlamydia trachomatis, either occurring alone or in association with other genital infections, were significantly greater in women using oral contracept ... | 1983 | 12338679 |
| the vaginal contraceptive pill. | vaginal administration of oral contraceptives (ocs) has been shown to be an acceptable and effective method of contraception. the most widely tested vaginal contraceptive pill (vcp) contains 500 mcg of d-1 norgestrel and 50 mcg of ethinyl estradiol (ee) and is administered on the same 21-day schedule as the oral contraceptive. other combination ocs used vaginally include 1 mg of norethisterone plus 50 mcg of mestranol, 250 mcg of levonorgestrel plus 50 mcg of ee, and 2 mg of cyproterone acetat ... | 1985 | 12339969 |
| trachomoniasis in imo state, nigeria: a first report. | the prevalence of trichomonas vaginalis infection in imo state of nigeria is reported and adds to the meagre knowledge on its distribution in the country. the study (august 1978-march 1981) is based on 794 high vaginal swabs (hvs), 178 male urethral swabs (mus) and 5883 male and female urine samples obtained from out-patients at the owerri general hospital as well as 447 hvs from adult out-patients from holy rosary hospital, emekuku. 10.2% of 1241 hvs, 3.4% of 178 mus and 1.3% of 5883 urine sa ... | 1984 | 12340185 |
| the vaginal flora of women in zimbabwe. | high vaginal and cervical swabs were taken from 199 pregnant women and 156 nonpregnant patients attending an std clinic in zimbabwe. trichomonas vaginalis was isolated from 31% of pregnant women and 37% of std patients. the bacterial flora in both groups was similar to that found in women from developed countries except that fecal organisms were more and lactobacilli less commonly isolated. n. gonorrheae was not isolated from any of the pregnant women, but was isolated from 13 of the std pati ... | 1983 | 12340187 |
| [vaginal microscopic evidence in women with symptoms of genital infection]. | vaginal living organisms have been defined as the most complete in human ecology. as many as 100 million colonies of microorganisms can live in a single ml of vaginal liquid. more common are the anaerobic types, particularly the lactobacillus and bacteroides. the low ph of the vagina, normally 4, is due to the high lactic acid content of the lactobacillus. pathogenic bacteria is inhibited at 4.5 ph. conversely, it proliferates at a high ph. the slightest alteration of this delicate equilib ... | 1985 | 12341331 |
| improved detection by dna amplification of trichomonas vaginalis in males. | trichomoniasis is a sexually transmitted infection that is highly prevalent worldwide and has been linked to preterm birth and human immunodeficiency virus acquisition. in females, trichomoniasis causes vaginitis, while in males, it is frequently asymptomatic but can be a cause of urethritis. control efforts have been hampered by the lack of a sensitive diagnostic technique for this infection in males. men attending a sexually transmitted disease (std) clinic for a new complaint were screened fo ... | 2002 | 12354865 |
| epidemiological features of women with trichomoniasis in auckland sexual health clinics: 1998-99. | to determine some epidemiological features of female sexually transmitted disease (std) clinic attendees with trichomonas vaginalis infection. | 2002 | 12362164 |
| resistance of trichomonas vaginalis infections to metronidazole in auckland sexual health clinics: report of two cases. | 2002 | 12362204 | |
| polyamine metabolism as chemotherapeutic target in protozoan parasites. | polyamines are essential cell constituents for all organisms. the present review highlights important differences in the synthesis, degradation, and interconversion of polyamines between the protozoan parasites (trypanosoma brucei, trypanosoma cruzi, cryptosporidium parvum and trichomonas vaginalis) and their mammalian hosts. approaches include development of mono- and di-substituted polyamine analogs targeting polyamine interconversion, as well as more traditional targeting of synthetic enzymes ... | 2002 | 12370040 |
| characterization of an ecto-phosphatase activity in the human parasite trichomonas vaginalis. | we have characterized phosphatase activity present on the external surface of trichomonas vaginalis, using intact living parasites. this enzyme hydrolyzes the substrate p-nitrophenylphosphate (p-npp) at a rate of 134.3+/-14.8 nmol pi/h per 10(7) cells. this phosphatase activity decreased by increasing the ph from 6.8 to 8.4, a ph range in which cell viability was maintained for at least 1 h. experiments using classical inhibitors of acid phosphatases, such as ammonium molybdate and sodium fluori ... | 2002 | 12375165 |
| the epidemiology of vaginal colonisation with group b streptococci in a sexually transmitted disease clinic. | to determine whether group b streptococcus (gbs) infection is sexually transmitted and whether colonisation with gbs could be related to vaginal symptoms or signs. | 2002 | 12381483 |
| biological variability in clinical isolates of trichomonas vaginalis. | eighteen clinical isolates of trichomonas vaginalis were obtained from women who attended health centers of the government of madrid. a total of 1,848 vaginal specimens recovered during the gynaecological examination were seeded in culture tubes containing liquid diamond medium. pathogenicity to mice was determined after intraperitoneal inoculation of mice by quantification of mortality and gross damage to abdominal organs. as could be expected, a broad variability was obtained, being some of th ... | 2002 | 12386717 |
| demonstration of trichomonas vaginalis in tracheal aspirates in infants with early respiratory failure. | trichomonas vaginalis was isolated from the tracheal aspirates of two premature newborns with early respiratory failure who were delivered vaginally by mothers with t. vaginalis infection. the babies were treated successfully with antiparasitic drugs. | 2002 | 12389679 |
| trichomonas vaginalis: observation of coexistence of multiple viruses in the same isolate. | trichomonas vaginalis is a flagellated, parasitic protozoan that inhabits the urogenital tract of humans. some isolates of t. vaginalis are infected with a double-stranded rna (dsrna) virus, which was described in the literature as homogeneous icosahedral viral particles with an isometric symmetry and 33 nm in diameter. this study examined in detail the viral particles in t. vaginalis isolate 347 and describes a heterogeneous population of viral particles. the different dsrna viruses were only o ... | 2002 | 12399035 |
| antimicrobial activity of dequalinium chloride against leading germs of vaginal infections. | dequalinium chloride (cas 522-51-0) and povidone iodine (cas 25655-41-8) are known as antiseptic agents and used in the local treatment of vaginal infections. clotrimazole (cas 23593-75-1) is an anti-fungal drug and applied primarily in the therapy of vulvo-vaginal candidiasis and to a lesser extent in bacterial vaginosis and trichomoniasis. however, antimicrobial activities of those three agents in comparison to each other have not been reported so far. to address this issue the antimicrobial a ... | 2002 | 12404886 |
| predictors of infection with trichomonas vaginalis: a prospective study of low income african-american adolescent females. | to identify psychosocial predictors of trichomonas vaginalis infection among low income african-american adolescent females living in a high risk urban area of the united states. | 2002 | 12407241 |
| post-treatment sexual and prevention behaviours of adolescents with sexually transmitted infections. | to evaluate sexual behaviour (including abstinence), sex partner change, and condom use during the 3 month period following treatment for neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, or non-gonococcal urethritis. | 2002 | 12407242 |
| does contraceptive method change the pap smear finding? | the aim of the present study was to investigate the effect of various types of contraceptives on morphological finding of pap smear results. pap smear results of 7,753 fertile women who presented to 15 different family planning services in fars province (south of iran) were analyzed according to their methods of contraception. the population consisted of 2241 women who used intrauterine device (iud) as a contraceptive method (group 1), 2521 women who were oral contraceptive (ocp) users (group 2) ... | 2002 | 12413619 |
| gang involvement and the health of african american female adolescents. | to examine the association between gang involvement and female adolescents' health. | 2002 | 12415063 |
| synthesis and in vitro antiprotozoal activity of 5-nitrothiophene-2-carboxaldehyde thiosemicarbazone derivatives. | several thiosemicarbazone derivatives of 5-nitrothiophene-2-carboxaldehyde were prepared by the simple process in which n(4)-thiosemicarbazone moiety was replaced by aliphatic, arylic and cyclic amine. among these thiosemicarbazones compound 11 showed significant antiamoebic activity whereas compound 3 was more active antitrichomonal than the reference drug. | 2002 | 12419387 |
| 'in vitro' study of chemotherapeutic activity of sulphimidazole on some sensitive and metronidazole-resistant trichomonas vaginalis strains. | trichomonacidal treatment based on 5-nitroimidazoles is problematic both when metronidazole, the drug of choice, is ineffective owing to the presence of resistant strains and when bacterial aerobic infections are present. sulphimidazole (siz) possesses two distinct functional groups: one sulphonamide, the other 5-nitroimidazole. since siz is active against aerobic and anaerobic bacteria, we set out to discover whether, in view of the presence of the 5-nitroimidazole group, it could also be effec ... | 2002 | 12419652 |
| evaluation of self-collected samples in contrast to practitioner-collected samples for detection of chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis by polymerase chain reaction among women living in remote areas. | self-collected samples have been shown to be an acceptable and sensitive method for the detection by polymerase chain reaction (pcr) of sexually transmitted infections (stis) among women. goal the goal of the study was to compare self-collected sampling methods to conventional practitioner endocervical sampling for the pcr detection of chlamydia trachomatis and neisseria gonorrhoeae to compare two self-collected sampling methods for the detection of t vaginalis by pcr. | 2002 | 12438900 |
| sexual intercourse association with asymptomatic bacterial vaginosis and trichomonas vaginalis treatment in relationship to preterm birth. | the purpose of this study was to determine whether sexual intercourse was associated with the treatment efficacy or the incidence of preterm birth in two large randomized trials in which metronidazole treatment of bacterial vaginosis or trichomonas vaginalis did not reduce preterm birth. | 2002 | 12439520 |
| an ectonucleotide atp-diphosphohydrolase activity in trichomonas vaginalis stimulated by galactose and its possible role in virulence. | this work describes the ability of living trichomonas vaginalis to hydrolyze extracellular atp (164.0 +/- 13.9 nmol pi/h x 10(7) cells). this ecto-enzyme was stimulated by zncl2, cacl2 and mgcl2, was insensitive to several atpase and phosphatase inhibitors and was able to hydrolyze several nucleotides besides atp. the activity was linear with cell density and with time for at least 60 min. the optimum ph for the t. vaginalis ecto-atpase lies in the alkaline range. d-galactose, known to be involv ... | 2002 | 12440729 |
| visualization of new virus-like-particles in trichomonas vaginalis. | in the present work, we demonstrate virus-like particles (vlps) with various morphological variations in trichomonas vaginalis. the vlps were distinct based on size, shape and electron density, with vlps being either electron-dense or electron-lucent. we used electron microscopy thin sections of several t. vaginalis strains virus-infected, and also negative staining of fractions obtained after purification by cscl buoyant density gradient centrifugation. the particles observed in fractions are i ... | 2002 | 12441093 |
| clinical effects of nifuratel in vulvovaginal infections. a meta-analysis of metronidazole-controlled trials. | nifuratel (cas 4936-47-4) displays a strong antiprotozoarian and antibacterial activity and is provided with certain fungicidal effect, but it is not active against the physiologic flora. its therapeutic effectiveness has been evaluated in more than 12,000 patients. the wide clinical experience with nifuratel confirms that the drug is safe and effective for the treatment of trichomoniasis, bacterial vaginosis, candidosis, and, particularly, in patients suffering from mixed vaginal infection. a m ... | 2002 | 12442634 |
| antitrichomonal strains of bacillus thuringiensis. | parasporal inclusion proteins from a total of 816 bacillus thuringiensis strains isolated in japan were examined for antitrichomonal activity against trichomonas vaginalis. ten strains of b. thuringiensis inhibited the growth of t. vaginalis in 48 h cultures at 37 degrees c. moreover, the b622 and b626 strains clearly showed trichomonacidal effects against t. vaginalis. the h antigen serotypes of both strains were identified as h13/29 (pakistani/amagiensis). the parasporal inclusion protein from ... | 2002 | 12444461 |
| evidence for lateral transfer of genes encoding ferredoxins, nitroreductases, nadh oxidase, and alcohol dehydrogenase 3 from anaerobic prokaryotes to giardia lamblia and entamoeba histolytica. | giardia lamblia and entamoeba histolytica are amitochondriate, microaerophilic protists which use fermentation enzymes like those of bacteria to survive anaerobic conditions within the intestinal lumen. genes encoding fermentation enzymes and related electron transport peptides (e.g., ferredoxins) in giardia organisms and amebae are hypothesized to be derived from either an ancient anaerobic eukaryote (amitochondriate fossil hypothesis), a mitochondrial endosymbiont (hydrogen hypothesis), or ana ... | 2002 | 12455953 |
| microbiology and management of polymicrobial female genital tract infections in adolescents. | this review describes the microbiology and management of female genital tract infections in adolescents. these infections include vulvovaginitis, vulvovaginal pyogenic infections (abscesses of bartholin's and skene's glands, infected labial inclusion cysts, labial abscesses, furunculosis, and hidradenitis), endometritis, pyometritis, salpingitis, pelvic inflammatory disease, and tubo-ovarian and pelvic abscess. anaerobes can be cultured in 50% to 90% of females with a variety of genital infectio ... | 2002 | 12459228 |
| chronic prostatitis caused by trichomonas vaginalis--diagnosis and treatment. | 2002 | 12462437 | |
| high prevalence of neisseria gonorrhoeae and multiple sexually transmitted diseases among rural women in the eastern highlands province of papua new guinea, detected by polymerase chain reaction. | in a previous community-based study among rural women in the eastern highlands province (ehp) of papua new guinea we determined that the prevalences of trichomonas vaginalis infection, chlamydia trachomatis infection, and syphilis were 46%, 26%, and 4%, respectively. surprisingly, however, the prevalence of neisseria gonorrhoeae infection was only 1%, which we considered low in consideration of the high prevalence of other sexually transmitted diseases (stds). the aim of the current study was to ... | 2002 | 12466719 |
| a novel potential surface protein in trichomonas vaginalis contains a leucine-rich repeat shared by micro-organisms from all three domains of life. | 2002 | 12467987 | |
| [prevalence of associated microorganisms in genital discharge, argentina]. | there was a significant increase in the number of women demanding gynecological care in public hospital of the great buenos aires, argentina, between 1997 and 1998. it was necessary to update the prevalence of associated microorganisms in order to review the laboratory support and adjust prevention and control guidelines. | 2002 | 12471378 |
| [retrospective microbiologic evaluation of vaginal cultures]. | vaginal culture is one of the most difficult cultures to be evaluated in clinical microbiology practice. the necessity of some expensive and complicated processes for diagnosis of some specific agents, age related variability of normal vaginal flora and failure caused by temporary presence of some pathogens in normal flora can be listed among the probable causes of that problem. in this study 8050 vaginal cultures performed in our hospital laboratories between 1 march 1999-15 september 2001 were ... | 2002 | 12476763 |
| [advances in the study of trichomoniasis in men]. | trichomoniasis is an important sexually transmitted disease that is associated with increased perinatal morbidity and increased hiv transmission. infection with trichomonas vaginalis also results in local urogenital tract symptoms. standard teaching is that trichomoniasis is an important cause of vaginitis in women, but that male sexual partners experience little or no morbidity. it is worth-while to summarize critical findings in a series of articles. the prevalence of trichomonas vaginalis in ... | 2002 | 12479053 |
| reactivity of reduced [2fe-2s] ferredoxins parallels host susceptibility to nitroimidazoles. | the kinetics of the electron transfer reaction between reduced [2fe-2s] ferredoxins and select nitroimidazole antimicrobial agents is reported. the ferredoxins from the protozoan trichomonas vaginalis and the cyanobacterium anabaena sp. strain 7120 were studied because they are the proximal electron donors to nitroimidazoles in these two organisms with significantly different nitroimidazole susceptibilities. the rates of electron transfer from anabaena ferredoxin to all nitroimidazoles were 1 to ... | 2003 | 12499206 |
| the chitinase system from trichomonas vaginalis as a potential target for antimicrobial therapy of urogenital trichomoniasis. | chitinolytic activities in trichomonas vaginalis membrane extracts were assessed by assays of three enzyme systems: n-acetyl-beta-d-hexosaminidase (nahase), chitobiosidase and chitotriosidase. n-acetyl-beta-d-hexosaminidase was the enzyme that showed the highest specific activity. after successive subcutaneous inoculations into mice and parasite recovery in culture, the enzyme activities increased significantly with the number of inoculations for up to eight passages. in addition, enzyme activit ... | 2002 | 12504272 |
| effect of the iron chelator deferoxamine on trichomonas vaginalis in vitro. | the effect of 10 micromol, 15 micromol, 30 micromol, and 60 micromol concentrations of deferoxamine (dfo), a clinically approved iron chelator, was determined on viability and multiplication of trichomonas vaginalis grown in tym axenic culture medium at 24 hours interval. dfo killed all t. vaginalis isolates with a minimum lethal concentration of 30 micromol after 48 hours culture incubation with the drug. a potent and persistent inhibitory effect of dfo on the parasite viability and multiplicat ... | 2002 | 12512803 |
| influence of hiv-1 coinfection on effective management of abnormal vaginal discharge. | reports on microbiologic cure rates following syndromic management (sm) of women with nonulcerative sexually transmitted infections (stis) are limited. | 2003 | 12514433 |
| validation of a urine-based pcr-enzyme-linked immunosorbent assay for use in clinical research settings to detect trichomonas vaginalis in men. | trichomonas vaginalis infection is highly prevalent worldwide and is associated with urethritis, prostatitis, and urethral strictures in men. however, the natural history and importance of t. vaginalis in men are poorly understood, in part because of difficulties in diagnosing infection. traditional detection methods rely on culture and wet-mount microscopy, which can be insensitive and time consuming. urethral swabs are commonly used to detect t. vaginalis in men, but discomfort from specimen c ... | 2003 | 12517867 |
| cytogenetic evaluation of two nitroimidazole derivatives. | 5-nitroimidazoles are a well-established group of antiprotozoal and antibacterial agents. thanks to their antimicrobial activity these chemotherapeutic agents inhibit the growth of both anaerobic bacteria and certain anaerobic protozoa such as trichomonas vaginalis, entamoeba histolytica and giardia lamblia. the aim of the present study is to achieve a precise characterization of the genotoxic activity of these compounds and to establish the value of cytogenetic assays in order to determine the ... | 2003 | 12537960 |
| prostate biopsy culture findings of men with chronic pelvic pain syndrome do not differ from those of healthy controls. | previous reports have identified bacteria in the prostate of men with chronic pelvic pain syndrome. to examine whether prostatic bacteria are more prevalent among patients with chronic pelvic pain syndrome than among those without pelvic pain, we compared 4-glass urine test and prostate biopsy results. | 2003 | 12544312 |
| self-collected vaginal swabs for the detection of multiple sexually transmitted infections in adolescent girls. | to evaluate the use of self-collected vaginal swabs to test for neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis, and to describe the acceptability of this technique to adolescents. | 2002 | 12547662 |
| urinary tract infection with trichomonas vaginalis in a premature newborn infant and the development of chronic lung disease. | we report a case of a low-birth-weight infant with an infection of the urinary tract with trichomonas vaginalis, who later developed cystic chronic lung disease suggestive of wilson-mikity syndrome. although she had mild respiratory distress syndrome at birth, the extent of the chronic lung disease was out of proportion to the initial illness. we speculate that maternal infection with this organism may have resulted in an inflammatory response that led to its development. | 2003 | 12556929 |
| diagnosis of symptomatic and asymptomatic trichomonas vaginalis infection by applying one tube nested pcr to vaginal discharge. | one tube nested-pcr targeting a species-specific tv-e650 repeat family of t. vaginalis genome, was applied to vaginal discharge specimen to diagnose symptomatic and asymptomatic trichomoniasis. the test was compared with axenic culture examination, wet mount preparation and papanicolaou stained smears. four-hundred and fifty cases symptomatic and symptomatic were collected over two years. out of 290 symptomatic women with cervicovaginitis and 160 asymptomatic women, 35 were culture positive for ... | 1999 | 12561939 |
| [a boy infant with trichomonas vaginalis infection of the urethra]. | 1999 | 12563773 | |
| effect of treatment for chlamydia trachomatis during pregnancy. | to screen and treat chlamydial infection in pregnant women in order to assess the effects of therapeutic intervention on the outcome of chlamydia trachomatis-infected pregnancy. | 2003 | 12566185 |
| [comparative effect of different media in in vitro cultivation of trichomonas vaginalis]. | to investigate the optimal condition for in vitro cultivation of trichomonas vaginalis for obtaining a better harvest of t. vaginalis. | 2002 | 12567545 |
| the alpha-actinin gene family: a revised classification. | the sequencing of a genome is the first stage of its complete characterization. subsequent work seeks to utilize available sequence data to gain a better understanding of the genes which are found within a genome. gene families comprise large portions of the genomes of higher vertebrates, and the available genomic data allow for a reappraisal of gene family evolution. this reappraisal will clarify relatedness within and between gene families. one such family, the alpha-actinin gene family, is pa ... | 2003 | 12569417 |
| [detection of dna of toxoplasma gondii in rat by using polymerase chain reaction]. | to study the significance of dna of toxoplasma gondii in peripheral blood. | 2001 | 12571947 |
| [antigen analysis of trichomonas vaginalis trophozoite by sds-page and two-dimensional gel electrophoresis]. | to analyze soluble antigens of trichomonas vaginalis. | 2001 | 12571968 |
| [a modified method for preparation of trichomonas vaginalis specimen]. | 2001 | 12572028 | |
| [studies on ultrastructure and histochemistry of trichomonas vaginalis adhering to vaginal mucosa of rats]. | to study interaction between trichomonas vaginalis and epithelium of genital tract of host as well as the pathogenesis of t. vaginalis. | 2001 | 12572044 |
| [association between mycoplasma genitalium and preterm labor]. | m. genitalium was identified by using polymerase chain reaction (pcr). the samples were obtained from the posterior vaginal fornix between 21-25 gestation weeks and investigated for ph, gram stain for bacterial vaginosis; chlamidia trachomartis; neisseria gonorrhoeae; trichomonas vaginalis. of 102 pregnant women, only five had a pcr-positive for m. genitalium. the occurrence of m. genitalium in posterior vaginal fornix at midtrimester is infrequent in pregnant women and unlikely to be a contribu ... | 2002 | 12577501 |
| infrequent parental monitoring predicts sexually transmitted infections among low-income african american female adolescents. | to prospectively determine (using an 18-month follow-up period) the association between african american female adolescents' perceptions of parental monitoring and their acquisition of biologically confirmed infection with chlamydia trachomatis, neisseria gonorrhoeae, and trichomonas vaginalis. | 2003 | 12580687 |
| 'shelf life' of trichomonas vaginalis. | the diagnosis of trichomonas vaginalis (tv) infection in women may be made by observing motile trichomonads in wet-mount preparations of vaginal discharge. the duration for which these organisms can be identified in such samples is unknown. we sought to assess this by performing wet-mount microscopy on samples from female patients immediately, and then in positive samples, every 10 minutes until motile trichomonads could no longer be identified. of the 65 wet-mount specimens positive for tv, a c ... | 2003 | 12590789 |
| accuracy of detection of trichomonas vaginalis organisms on a liquid-based papanicolaou smear. | the study was undertaken to determine the accuracy of the liquid-based papanicolaou (pap) smear in the detection of trichomonas vaginalis organisms in women. | 2003 | 12592239 |
| agents of non-gonococcal urethritis in males attending an israeli clinic for sexually transmitted diseases. | non-gonococcal urethritis is the most common clinical diagnosis in men seeking care at clinics for sexually transmitted diseases. | 2003 | 12592953 |
| a family history of deoxyribonuclease ii: surprises from trichinella spiralis and burkholderia pseudomallei. | deoxyribonuclease iialpha (dnase iialpha) is an acidic endonuclease found in lysosomes and nuclei, and it is also secreted. though its caenorhabditis elegans homolog, nuc-1, is required for digesting dna of apoptotic cell corpses and dietary dna, it is not required for viability. however, dnase iialpha is required in mice for correct development and viability, because undigested cell corpses lead to lesions throughout the body. recently, we showed that, in contrast to previous reports, active dn ... | 2003 | 12594037 |
| reproductive tract infections among married women in upper egypt. | in a house-to-house survey using cluster sampling, 1344 married women from urban and rural areas of upper egypt (minia, assiut and sohag) were interviewed and examined to study the magnitude and determinants of reproductive tract infections. overall prevalence was found to be 52.8%, with the most prevalent forms being candida albicans (28.0%), trichomonas vaginalis (8.7%), aspergillus species (7.4%), streptococci (4.6%) and chlamydia trachomatis (4.2%). multivariate analysis identified certain g ... | 2001 | 12596963 |
| iron and contact with host cells induce expression of adhesins on surface of trichomonas vaginalis. | the proteins ap65, ap51, ap33 and ap23 synthesized by trichomonas vaginalis organisms in high iron play a role in adherence. multigene families encode enzymes of the hydrogenosome organelles, which have identity to adhesins. this fact raises questions regarding the compartmentalization of the proteins outside the organelle and about the interactions of adhesins with host cells. data here demonstrate the presence of the proteins outside the organelle under high-iron conditions. fluorescence and i ... | 2003 | 12603729 |
| [systematic search for parasites among leprosy patients in mali]. | practice of multidrug therapy in leprosy (combination dapsone + rifampicine + clofazimine) established since 1981, has significantly reduced the incidence of the disease. however, immunosuppression due to treatment of multi-drugs therapy induced adverse reactions with glucocorticoid and the change in host immune response due to the leprosy itself, might increase the risk of parasitic infections. to test this hypothesis, we carried out a case-control study at the "institut marchoux" in bamako. st ... | 2002 | 12616942 |
| trichomoniasis: what's new? | trichomonas vaginalis is the most common nonviral sexually transmitted disease in the world. recent research has elucidated several virulence factors that allow t. vaginalis to adhere to extracellular host proteins and evade the host immune system. further investigation is needed to determine the mechanisms responsible for the perinatal morbidity associated with trichomoniasis and to evaluate the interactions between trichomoniasis and hiv infection. an effort to decrease t. vaginalis associated ... | 2003 | 12641999 |
| sexual behavior of hiv discordant couples after hiv counseling and testing. | sexual behavior following voluntary hiv counseling and testing (vct) is described in 963 cohabiting heterosexual couples with one hiv positive and one hiv negative partner ('discordant couples'). biological markers were used to assess the validity of self-report. | 2003 | 12646797 |
| role of the vaginal microbiological ecosystem and cytokine profile in the promotion of cervical dysplasia: a case-control study. | to identify alterations in the cytokine profile and microbial ecosystem of the vagina in association with cervical dysplasia. | 2002 | 12648311 |
| in vitro metronidazole and tinidazole activities against metronidazole-resistant strains of trichomonas vaginalis. | the in vitro activities of tinidazole and metronidazole against trichomonas vaginalis isolates clinically resistant to metronidazole were compared. minimal lethal concentrations (mlcs) of tinidazole were significantly lower than mlcs of metronidazole. increased metronidazole resistance correlated with increased tinidazole resistance. these data support a role for tinidazole in the treatment of trichomoniasis. | 2003 | 12654679 |
| is bacterial vaginosis associated with cervical intraepithelial neoplasia? | previous research has produced conflicting results regarding the association of bacterial vaginosis (bv) and cervical intraepithelial neoplasia (cin). these studies have been weakened in their conclusions mainly by failure to adequately control for the presence of sexually transmitted infections (stis). one proposed mechanism suggesting that carcinogenic nitrosamines acting either independently or via human papilloma virus (hpv) has not been fully tested previously. we undertook a prospective, c ... | 2003 | 12657117 |
| morphogenesis during division and griseofulvin-induced changes of the microtubular cytoskeleton in the parasitic protist, trichomonas vaginalis. | the behavior of microtubular structures during division was followed by immunofluorescence in trichomonas vaginalis using an anti-alpha-tubulin monoclonal antibody together with nuclear staining by dapi, allowing us to describe successive mitotic stages. in contrast to recent reports, we showed that: (1) the microtubular axostyle-pelta complex depolymerized during division, (2) the flagella were assembled during mitosis, and (3) the flagellar number was restored in each daughter kinetid before c ... | 2003 | 12658461 |
| trichomonas vaginalis: identification of a phospholipase a-dependent hemolytic activity in a vesicular subcellular fraction. | trichomonad total extracts (tte), or vesicular (p30) and soluble (530) subcellular fractions from 3 pathogenic trichomonas vaginalis strains (gt-3. gt-13. and gt-15), lysed both human and sprague-dawley rat erythrocytes in a time- and dose-dependent manner. the entire hemolytic activity of tte was located in p30, showing 2 peaks of maximum activity, one at ph 6.0 and another at ph 8.0. in the presence of 1 mm ca2+. hemolytic activity on rat erythrocytes was greater at ph 6.0 16.71 +/- 0.33 hemol ... | 2003 | 12659311 |
| synthesis, antiprotozoal and anticancer activity of substituted 2-trifluoromethyl- and 2-pentafluoroethylbenzimidazoles. | the synthesis of several halogenated benzimidazoles substituted in position 2 with trifluoromethyl, pentafluoroethyl and 2-thioethylaminodimethyl group is reported. antiprotozoal and anticancer activity of series of newly synthesized and previously obtained compounds was studied. all of tested bezimidazoles showed remarkable antiprotozoal activity against giardia intestinalis, entamoeba histolytica and trichomonas vaginalis. of the studied collection of halogenated benzimidazoles the most antica ... | 2002 | 12660022 |
| should we offer antibiotic prophylaxis post sexual assault? | our objective was to assess whether antibiotic prophylaxis should be offered to women post sexual assault by considering acceptability of prophylaxis, follow up attendance rates and the prevalence of sexually transmitted infections (stis) in these women. retrospective case notes review of female survivors of rape or sexual assault attending the rose clinic, ambrose king centre, royal london hospital between 1 january 1997 and 31 may 1999 was carried out. the following selection criteria were app ... | 2003 | 12662387 |
| modulation by polypodium leucotomos extract of cytokine patterns in experimental trichomoniasis model. | the immunomodulating effects of anapsos, an aqueous hydrosoluble extract obtained from the rhizomes of the fern polypodium leucotomos, on both pathogenicity and cytokine levels in serum (ifn-gamma/il-4) were assayed in a trichomonas vaginalis experimental model (balb/c mice infected with 10(7) trichomonads and examined at day 15 after infection). doses of 20 mg/kg/day administered for 10 days before the infection with the parasite induced a decrease of the experimental pathogenicity approximatel ... | 2003 | 12669353 |
| molecular biology of the amitochondriate parasites, giardia intestinalis, entamoeba histolytica and trichomonas vaginalis. | the amitochondriates are an assembly of unicellular protists that lack mitochondria, and often other typical eukaryotic organelles, such as peroxisomes. relatively little research has been conducted on amitochondriates, even though marine waters are likely to be teeming with such organisms, representing yet unknown protist lineages. the three amitochondriates that have been studied in any detail are the three mucosal human parasites: giardia, trichomonas and entamoeba. these pathogens have world ... | 2003 | 12670510 |
| assessment of the validity of and adherence to sexually transmitted infection algorithms at a female sex worker clinic in abidjan, côte d'ivoire. | algorithms for sexually transmitted infection (sti) case management were designed in a female sex worker (fsw) clinic in abidjan, côte d'ivoire, in 1993. | 2003 | 12671546 |
| adenosine is the primary precursor of all purine nucleotides in trichomonas vaginalis. | trichomonas vaginalis, a parasitic protozoan and the causative agent of trichomoniasis, lacks de novo purine nucleotide synthesis and possesses a unique purine salvage pathway, consisting of a bacterial type purine nucleoside phosphorylase and a purine nucleoside kinase. it is generally believed that adenine and guanine are converted to their corresponding nucleosides and then further phosphorylated to form amp and gmp, respectively, as the main as well as the essential pathway of replenishing t ... | 2003 | 12672523 |
| trichomonas vaginalis: in vitro attachment and internalization of hiv-1 and hiv-1-infected lymphocytes. | sexually transmitted diseases (stds) caused by bacteria and protozoa play an important role in the epidemiology of human immunodeficiency virus (hiv-1) infection. human trichomoniasis, produced by the protozoan parasite trichomonas vaginalis, is one of the most common stds, and is a cause of mucosal lesions in the urogenital tract, which may increase the risk for hiv infection. however, there are no reports concerning the outcome of in vitro interactions between hiv particles and trichomonads. t ... | 2003 | 12674478 |
| prevalence of asymptomatic genital infection among pregnant women in benin city, nigeria. | the prevalence of asymptomatic genital infection among pregnant women and their susceptibility to antibacterial agents was investigated to provide baseline data on common asymptomatic genital microorganisms and identify potentials for development of clinical disease among this cohort of patients. high vaginal swabs were obtained from five hundred consecutive and consenting pregnant women attending the antenatal clinic of the university of benin teaching hospital (ubth) and the central hospital, ... | 2002 | 12685413 |
| use of urine polymerase chain reaction to define the prevalence and clinical presentation of trichomonas vaginalis in men attending an std clinic. | to determine the prevalence and clinical features of trichomonas vaginalis (tv) infection in men. | 2003 | 12690140 |
| trichomonas vaginalis: microtubule cytoskeleton distribution using fluorescent taxoid. | trichomonas vaginalis is a flagellated parasitic protist of the human urogenital tract. the parasite has a poorly known cytoskeleton formed by an axostyle and a pelta, which are formed by stable structures such as microtubules, essential for the maintenance of cell shape and organization. flutax-2 is an active fluorescent derivative of taxol, binds to alphabeta-tubulin dimer polymerized. in this paper we present the analysis of microtubule distribution in living trophozoites of t. vaginalis usin ... | 2002 | 12706747 |
| tetracycline-inducible gene expression in trichomonas vaginalis. | transient and stable gene delivery systems are available for trichomonas vaginalis, however, they do not allow regulated expression of target genes. to study essential genes or proteins that are toxic to the cells when over expressed, we have developed an inducible/repressible gene expression system in this parasite, which is driven by the tet-operator (teto) and regulated tetracycline-responsive tet repressor (tetr). inducible chloramphenicol acetyl transferase (cat) gene expression is observed ... | 2003 | 12706795 |
| antitrichomonas in vitro activity of cussonia holstii engl. | cussonia holstii is a traditional medicinal plant used in kenya, which has not been studied chemically up-to-date. upon biological screening, a substantial antitrichomonas activity was found in the dichloromethane extract of the bark of cussonia holstii. a detailed chromatographic study using centrifugal partition chromatography revealed that a pentacyclic triterpenoid, i.e., hederagenin, is the antitrichomonas constituent of the plant with a ic50 of 2.8 microm. | 2003 | 12713125 |
| identification of trichomonas vaginalis in papanicolaou smears by fluorescence microscopy. | 2003 | 12713485 | |
| geographical variations in the prevalence of hiv and other sexually transmitted infections in rural tanzania. | the prevalence and incidence of hiv and other sexually transmitted infections (stis) were recorded in two lake-island and eight rural mainland communities in mwanza, tanzania. the prevalence and incidence of hiv and syphilis were lower on the islands, but this pattern was not seen for herpes simplex virus type-2, gonorrhoea, chlamydia, male urethritis or antenatal prevalences of trichomonas vaginalis. island men reported fewer sexual partners than mainland men but no differences were found for w ... | 2003 | 12716499 |
| evaluation of an igg cystatin capture enzyme-linked immunosorbent assay for the detection of anti-cysteine proteinase antibodies in asymptomatic trichomoniasis patients. | all isolates of t. vaginalis release cysteine proteinases proteolytic enzymes that are shed into the vagina or culture medium. cystatin has been used successfully as a capture agent in elisa to detect cysteine proteinase antibodies without the need for purified proteinases. the elisa was evaluated in comparison to wet mount microscopy and culture techniques. igg cystatin capture elisa proved to be a sensitive and highly specific (100%) assay that could rapidly detect anti-cysteine proteinase ant ... | 2003 | 12739802 |
| perspective of a new diagnostic for human trichomonosis. | several diagnostic techniques have been employed for the detection of trichomonas vaginalis. microtubules constitute the cytoskeleton in eukaryotic cells and are sensitive to antimitotic drugs, such as taxol (paclitaxel). we used flutax a fluorescent taxoid - to analyze the microtubule distribution in living trophozoites of t. vaginalis in urine and in vaginal discharge. a high intensity of fluorescence was observed in living t. vaginalis, epithelial cells and leukocytes present in urine and vag ... | 2003 | 12764446 |
| trichomonas vaginalis hmp35, a putative pore-forming hydrogenosomal membrane protein, can form a complex in yeast mitochondria. | an abundant integral membrane protein, hmp35, has been isolated from hydrogenosomes of trichomonas vaginalis. this protein has no known homologue and exists as a stable 300-kda complex, termed hmp35, in membranes of the hydrogenosome. by using blue native gel electrophoresis, we found the hmp35 complex to be stable in 2 m nacl and up to 5 m urea. the endogenous hmp35 protein was largely protease-resistant. the protein has a predominantly beta-sheet structure and predicted transmembrane domains t ... | 2003 | 12766161 |
| value of consistent condom use: a study of sexually transmitted disease prevention among african american adolescent females. | 2003 | 12773349 | |
| addition of treatment for trichomoniasis to syndromic management of urethritis in malawi: a randomized clinical trial. | male urethritis is generally treated syndromically, but failure of empirical treatment is common. | 2003 | 12782954 |
| knowledge, attitude and practice about sexually transmitted diseases among university students in kampala. | sexually transmitted diseases (stds) remain an important cause of morbidity and mortality among women in the child-bearing age. in order to institute appropriate preventive measures there is need to establish the profile of knowledge of the predisposing factors and causation of stds, attitude to sexual practice and sexual patterns among the susceptible young people, such as university students. | 2001 | 12789128 |
| should family planning clinics provide clinical services for sexually transmitted infections? a case study from côte d'ivoire. | to evaluate the quality and usefulness of integrated sexually transmitted infection (sti) care at non-governmental family planning (fp) clinics in côte d'ivoire. | 2003 | 12791061 |
| pulmonary coinfection by trichomonas vaginalis and pneumocystis sp. as a novel manifestation of aids. | a 41-year-old man was hospitalized, presenting increasing dyspnea and extensive ground-glass opacities on chest x-ray. infection by human immunodeficiency virus was confirmed. cytologic examination of bronchoalveolar lavage fluid revealed numerous trichomonads and aggregates of pneumocystis sp. treatment was followed by rapid improvement of respiratory symptoms and chest x-ray. the trichomonad species found in the lungs was identified as trichomonas vaginalis by small-subunit rrna gene amplifica ... | 2003 | 12792927 |
| late recurrence of resistant trichomonas vaginalis vaginitis: relapse or re-infection? | distinguishing between re-infection and relapse of trichomonas infections is often a difficult task in the clinical setting. the chronicity of trichomonas infections and the ongoing sexual activity are two confounding factors. we present a patient with recurrent resistant vaginal trichomoniasis shortly following a sexual contact with an untreated partner after a complete response to treatment with tinidazole for nine months. we hypothesise that re-infection occurred from the asymptomatic partner ... | 2003 | 12794219 |
| was the papanicolaou smear responsible for the decline of trichomonas vaginalis? | 2003 | 12794224 | |
| optimization of random amplified polymorphic dna techniques for its use in genetic studies of trichomonas vaginalis isolates. | the random amplified polymorphic dna (rapd) technique is a simple method to detect dna polymorphism. several factors can affect the amplification profiles causing the presence of false bands and assay non-reproducibility. in this study, we analyzed the effect of changing concentrations of the primer, template dna and taq dna polymerase with the goal of determining their optimum concentration for the standardization of the rapd technique for genetic studies of trichomonas vaginalis, a parasite th ... | 2002 | 12798003 |
| interventions for treating trichomoniasis in women. | around 120 million women worldwide suffer from trichomonas vaginalis vaginitis every year. the infection is sexually transmitted and is believed to facilitate hiv transmission. | 2003 | 12804391 |
| is there a seasonal difference in the detection of trichomonas vaginalis by cervical cytology? | this objective of our study was to determine the prevalence of a trichomonas vaginalis diagnosis in routine papanicolaou smears and whether it is seasonal. we reviewed the diagnosis rendered for 93,681 papanicolaou smears evaluated at a medical school hospital laboratory between 1992 and 1997. the occurrence of a diagnosis of t. vaginalis was analyzed by year, by quarter, and by month using a generalized linear regression model. the prevalence of a t. vaginalis diagnosis during the 6-year study ... | 2003 | 12806119 |
| trichomonas vaginalis: evaluating capsid proteins of dsrna viruses and the dsrna virus within patients attending a sexually transmitted disease clinic. | some isolates of trichomonas vaginalis, the number one, non-viral sexually transmitted disease agent, are infected with one or several distinct double stranded (ds)-rna virus. immune rabbit anti-capsid serum (irs) reacted with the capsid protein of purified dsrna virus of a subset of the virus-infected t. vaginalis isolates. a monoclonal antibody (mab) that recognized the capsid protein reactive with the irs was generated. analysis of the virus capsid protein of virus-infected isolates by probin ... | 2003 | 12810045 |
| double stranded rna virus in south african trichomonas vaginalis isolates. | to screen trichomonas vaginalis isolates from south africa for the presence of a small double stranded rna virus designated t vaginalis virus (tvv). | 2003 | 12835302 |
| antibacterial activity of artocarpus heterophyllus. | the crude methanolic extracts of the stem and root barks, stem and root heart-wood, leaves, fruits and seeds of artocarpus heterophyllus and their subsequent partitioning with petrol, dichloromethane, ethyl acetate and butanol gave fractions that exhibited a broad spectrum of antibacterial activity. the butanol fractions of the root bark and fruits were found to be the most active. none of the fractions were active against the fungi tested. | 2003 | 12837372 |