Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| characterization of two brassinosteroid c-6 oxidase genes in pea. | c-6 oxidation genes play a key role in the regulation of biologically active brassinosteroid (br) levels in the plant. they control br activation, which involves the c-6 oxidation of 6-deoxocastasterone (6-deoxocs) to castasterone (cs) and in some cases the further conversion of cs to brassinolide (bl). c-6 oxidation is controlled by the cyp85a family of cytochrome p450s, and to date, two cyp85as have been isolated in tomato (solanum lycopersicum), two in arabidopsis (arabidopsis thaliana), one ... | 2007 | 17322341 |
| roles for rice membrane dynamics and plasmodesmata during biotrophic invasion by the blast fungus. | rice blast disease is caused by the hemibiotrophic fungus magnaporthe oryzae, which invades living plant cells using intracellular invasive hyphae (ih) that grow from one cell to the next. the cellular and molecular processes by which this occurs are not understood. we applied live-cell imaging to characterize the spatial and temporal development of ih and plant responses inside successively invaded rice (oryza sativa) cells. loading experiments with the endocytotic tracker fm4-64 showed dynamic ... | 2007 | 17322409 |
| a genome-wide transcriptional activity survey of rice transposable element-related genes. | transposable element (te)-related genes comprise a significant portion of the gene catalog of grasses, although their functions are insufficiently characterized. the recent availability of te-related gene annotation from the complete genome sequence of rice (oryza sativa) has created an opportunity to conduct a comprehensive evaluation of the transcriptional activities of these potentially mobile elements and their related genes. | 2007 | 17326825 |
| a database of pcr primers for the chloroplast genomes of higher plants. | chloroplast genomes evolve slowly and many primers for pcr amplification and analysis of chloroplast sequences can be used across a wide array of genera. in some cases 'universal' primers have been designed for the purpose of working across species boundaries. however, the essential information on these primer sequences is scattered throughout the literature. | 2007 | 17326828 |
| a comprehensive crop genome research project: the superhybrid rice genome project in china. | in may 2000, the beijing institute of genomics formally announced the launch of a comprehensive crop genome research project on rice genomics, the chinese superhybrid rice genome project. srgp is not simply a sequencing project targeted to a single rice (oryza sativa l.) genome, but a full-swing research effort with an ultimate goal of providing inclusive basic genomic information and molecular tools not only to understand biology of the rice, both as an important crop species and a model organi ... | 2007 | 17331896 |
| involvement of polyamines in the drought resistance of rice. | this study investigated whether and how polyamines (pas) in rice (oryza sativa l.) plants are involved in drought resistance. six rice cultivars differing in drought resistance were used and subjected to well-watered and water-stressed treatments during their reproductive period. the activities of arginine decarboxylase, s-adenosyl-l-methionine decarboxylase, and spermidine (spd) synthase in the leaves were significantly enhanced by water stress, in good agreement with the increase in putrescine ... | 2007 | 17332417 |
| nitrite-driven anaerobic atp synthesis in barley and rice root mitochondria. | mitochondria isolated from the roots of barley (hordeum vulgare l.) and rice (oryza sativa l.) seedlings were capable of oxidizing external nadh and nadph anaerobically in the presence of nitrite. the reaction was linked to atp synthesis and nitric oxide (no) was a measurable product. the rates of nadh and nadph oxidation were in the range of 12-16 nmol min(-1) mg(-1) protein for both species. the anaerobic atp synthesis rate was 7-9 nmol min(-1) mg(-1) protein for barley and 15-17 nmol min(-1) ... | 2007 | 17333252 |
| experimental functional response and inter-individual variation in foraging rate of teal (anas crecca). | the functional response, i.e. the change in per capita food intake rate per time unit with changed food availability, is a widely used tool for understanding the ecology and behaviour of animals. however, waterfowl remain poorly explored in this context. in an aviary experiment we derived a functional response curve for teal (anas crecca) foraging on rice (oryza sativa) seeds. we found a linear relationship between intake rate and seed density, as expected for a filter-feeder. at high seed densi ... | 2007 | 17336000 |
| rice (oryza sativa) allergy in rhinitis and asthma patients: a clinico-immunological study. | sensitization to foods varies in different countries reflecting a possible interaction of genetic factors, cultural and dietary habits. rice is a major food consumed world wide and needs evaluation for ige mediated reactions. the present study was carried out to identify rice allergy in patients of rhinitis and asthma and identify the allergenic proteins in raw and cooked rice. of 1200 patients screened using standard questionnaire, 165 presented with history of rice allergy. of these, 20 (12.1% ... | 2007 | 17336834 |
| an unusual posttranscriptional processing in two betaine aldehyde dehydrogenase loci of cereal crops directed by short, direct repeats in response to stress conditions. | various abilities to synthesize and accumulate glycine betaine (gb) are crucial for angiosperms to develop salt and drought tolerances. in higher plants, gb is synthesized by a two-step oxidation of choline via an intermediate form of betaine aldehyde, and catalyzed by choline monooxygenase and betaine aldehyde dehydrogenase (badh). in this study, numerous truncated and/or recombinant transcripts of two badh homologs resulting from an unusual posttranscriptional processing were detected in rice ... | 2007 | 17337526 |
| the flowering locus t-like gene family in barley (hordeum vulgare). | the flowering locus t (ft) gene plays a central role in integrating flowering signals in arabidopsis because its expression is regulated antagonistically by the photoperiod and vernalization pathways. ft belongs to a family of six genes characterized by a phosphatidylethanolamine-binding protein (pebp) domain. in rice (oryza sativa), 19 pebp genes were previously described, 13 of which are ft-like genes. five ft-like genes were found in barley (hordeum vulgare). hvft1, hvft2, hvft3, and hvft4 we ... | 2007 | 17339225 |
| comparative physical mapping between oryza sativa (aa genome type) and o. punctata (bb genome type). | a comparative physical map of the aa genome (oryza sativa) and the bb genome (o. punctata) was constructed by aligning a physical map of o. punctata, deduced from 63,942 bac end sequences (bess) and 34,224 fingerprints, onto the o. sativa genome sequence. the level of conservation of each chromosome between the two species was determined by calculating a ratio of bes alignments. the alignment result suggests more divergence of intergenic and repeat regions in comparison to gene-rich regions. fur ... | 2007 | 17339227 |
| osmtn encodes a 5'-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice (oryza sativa l.). | methylthioadenosine (mta) is released as a by-product of s-adenosylmethionine (adomet)-dependent reactions central to ethylene, polyamine, or phytosiderophore biosynthesis. mta is hydrolysed by methylthioadenosine nucleosidase (mtn; ec 3.2.2.16) into adenine and methylthioribose which is processed through the methionine (met) cycle to produce a new molecule of adomet. in deepwater rice, submergence enhances ethylene biosynthesis, and ethylene in turn influences the methionine cycle through posit ... | 2007 | 17339651 |
| arsenic accumulation in rice (oryza sativa l.): human exposure through food chain. | although human exposure to arsenic is thought to be caused mainly through arsenic-contaminated underground drinking water, the use of this water for irrigation enhances the possibility of arsenic uptake into crop plants. rice is the staple food grain in bangladesh. arsenic content in straw, grain and husk of rice is especially important since paddy fields are extensively irrigated with underground water having high level of arsenic concentration. however, straw and husk are widely used as cattle ... | 2008 | 17346792 |
| genebins: a database for classifying gene expression data, with application to plant genome arrays. | to interpret microarray experiments, several ontological analysis tools have been developed. however, current tools are limited to specific organisms. | 2007 | 17349060 |
| expression of mitochondrial tatc in nicotiana tabacum is responsive to benzothiadiazole and salicylic acid. | a cdna, up-regulated upon treatment of tobacco cells with salicylic acid and benzo (1,2,3) thiadiazole-7-carbothioic acid s-methyl ester, was identified by differential rna display and the full sequence obtained. this mitochondrial gene, twin arginine translocation (tatc), resembles orthologues across different species, including the gene that codes for a sec-independent membrane translocating protein in bacteria. hypothetical tatc proteins have also been identified in the mitochondria of arabid ... | 2007 | 17350139 |
| assimilation of ammonium ions and reutilization of nitrogen in rice (oryza sativa l.). | a major source of inorganic nitrogen for rice plants grown in paddy soil is ammonium ions. the ammonium ions are actively taken up by the roots via ammonium transporters and subsequently assimilated into the amide residue of glutamine (gln) by the reaction of glutamine synthetase (gs) in the roots. the gln is converted into glutamate (glu), which is a central amino acid for the synthesis of a number of amino acids, by the reaction of glutamate synthase (gogat). although a small gene family for b ... | 2007 | 17350935 |
| a wuschel-like homeobox gene represses a yabby gene expression required for rice leaf development. | yabby and wuschel-like homeobox (wox) genes have been shown to play important roles in lateral organ formation and meristem function. here, we report the characterization of functional relationship between rice (oryza sativa) yab3 and wox3 in rice leaf development. rice yab3 is closely related to maize (zea mays) zmyab14 and arabidopsis (arabidopsis thaliana) filamentous flower (fil), whereas rice wox3 is highly conserved with maize narrow sheath1 (ns1) and ns2 and arabidopsis pressed flower (pr ... | 2007 | 17351053 |
| magnaporthe grisea infection triggers rna variation and antisense transcript expression in rice. | rice blast disease, caused by the fungal pathogen magnaporthe grisea, is an excellent model system to study plant-fungal interactions and host defense responses. in this study, comprehensive analysis of the rice (oryza sativa) transcriptome after m. grisea infection was conducted using robust-long serial analysis of gene expression. a total of 83,382 distinct 21-bp robust-long serial analysis of gene expression tags were identified from 627,262 individual tags isolated from the resistant (r), su ... | 2007 | 17351054 |
| a novel bioinformatics approach identifies candidate genes for the synthesis and feruloylation of arabinoxylan. | arabinoxylans (axs) are major components of graminaceous plant cell walls, including those in the grain and straw of economically important cereals. despite some recent advances in identifying the genes encoding biosynthetic enzymes for a number of other plant cell wall polysaccharides, the genes encoding enzymes of the final stages of ax synthesis have not been identified. we have therefore adopted a novel bioinformatics approach based on estimation of differential expression of orthologous gen ... | 2007 | 17351055 |
| an expression atlas of rice mrnas and small rnas. | identification of all expressed transcripts in a sequenced genome is essential both for genome analysis and for realization of the goals of systems biology. we used the transcriptional profiling technology called 'massively parallel signature sequencing' to develop a comprehensive expression atlas of rice (oryza sativa cv nipponbare). we sequenced 46,971,553 mrna transcripts from 22 libraries, and 2,953,855 small rnas from 3 libraries. the data demonstrate widespread transcription throughout the ... | 2007 | 17351617 |
| characterization and identification of microrna core promoters in four model species. | micrornas are short, noncoding rnas that play important roles in post-transcriptional gene regulation. although many functions of micrornas in plants and animals have been revealed in recent years, the transcriptional mechanism of microrna genes is not well-understood. to elucidate the transcriptional regulation of microrna genes, we study and characterize, in a genome scale, the promoters of intergenic microrna genes in caenorhabditis elegans, homo sapiens, arabidopsis thaliana, and oryza sativ ... | 2007 | 17352530 |
| effect of 2,4-d, hydric stress and light on indica rice (oryza sativa) somatic embryogenesis. | with the purpose of increasing the embryogenesis regeneration process in vitroplants obtained from somatic embryos of the indica rice variety cr-5272 (oryza sativa l.), two independent experiments were performed. the first experiment consisted in the effect of combination of three concentrations of the gelling agent phytagel (1.8. 2.4. and 3 gl(-1)) and four 2,4-d concentrations (2.26, 4.52. 6.78, and 9.05 microm) on the induction and subsequent regeneration of embryogenic calli. on the second e ... | 2015 | 17354447 |
| bayesian association mapping of multiple quantitative trait loci and its application to the analysis of genetic variation among oryza sativa l. germplasms. | one way to use a crop germplasm collection directly to map qtls without using line-crossing experiments is the whole genome association mapping. a major problem with association mapping is the presence of population structure, which can lead to both false positives and failure to detect genuine associations (i.e., false negatives). particularly in highly selfing species such as asian cultivated rice, high levels of population structure are expected and therefore the efficiency of association map ... | 2007 | 17356864 |
| establishment of an in vitro fertilization system in rice (oryza sativa l.). | in vitro fertilization (ivf) systems using isolated male and female gametes have been utilized to dissect fertilization-induced events in angiosperms, such as egg activation, zygote development and early embryogenesis, as the female gametophytes of plants are deeply embedded within ovaries. in this study, a rice ivf system was established to take advantage of the abundant resources stemming from rice research for investigations into the mechanisms of fertilization and early embryogenesis. fusion ... | 2007 | 17361458 |
| [nanoscale silicas in oryza sativa l. and their uv absorption]. | to reveal the unique microstructure of silica in rice and its absorption of ultra violet, wet digestion was chosen to isolate silica bodies from rice leaves and bract according to the fact that concentrated acid cannot destroy glass made of sio2. a mixed solution of sulfuric and nitric acids was applied to the leaves and bract of rice, respectively. after keeping the treated samples in 60-70 "c water bath for 30 hours and times of washing and sedimentation in water, pure silica bodies were obtai ... | 2006 | 17361739 |
| molecular dissection of developmental behavior of tiller number and plant height and their relationship in rice (oryza sativa l.). | plant height and tiller number are two important characters related to yield in rice (oriza sativa l.). zhenshan97 x minghui63 recombinant inbred lines were employed to dissect the genetic basis of development of plant height and tiller number using conditional and unconditional composite interval mapping approaches. the traits were normally distributed with transgressive segregation in both directions. increasingly negative correlations were observed between tiller number and plant height at fi ... | 2006 | 17362360 |
| overexpression of salicylic acid carboxyl methyltransferase reduces salicylic acid-mediated pathogen resistance in arabidopsis thaliana. | we cloned a salicylic acid/benzoic acid carboxyl methyltransferase gene, osbsmt1, from oryza sativa. a recombinant osbsmt1 protein obtained by expressing the gene in escherichia coli exhibited carboxyl methyltransferase activity in reactions with salicylic acid (sa), benzoic acid (ba), and de-s-methyl benzo(1,2,3)thiadiazole-7-carbothioic acid (dsm-bth), producing methyl salicylate (mesa), methyl benzoate (meba), and methyl dsm-bth (mebth), respectively. compared to wild-type plants, transgenic ... | 2007 | 17364223 |
| cloning and sequence analysis of germin-like protein gene 2 promoter from oryza sativa l. ssp. indica. | germin and germin-like proteins (glps) are water soluble extracellular proteins reportedly expressed in response to some environmental and developmental signals. some enzymatic activities have also been associated with germin/glps. however, their role in overall metabolism has not been fully understood. significant insight into their function may also be gained by analysis of their promoter. during this study, about 1107 bp 5'region of osrglp2 gene was amplified, cloned and sequenced. the sequen ... | 2007 | 17364810 |
| assessment of reclaimed wastewater irrigation impacts on water quality, soil, and rice cultivation in paddy fields. | the objective of this research was to monitor and assess the impact of reclaimed wastewater irrigation on water quality, soil, and rice cultivation by comparing the effects of various wastewater treatment levels on the growth and yield of rice. a randomized complete block design was used for the application methods of the wastewater effluents to paddy rice, with five treatments and six replications. the treatments were: control with groundwater irrigation (gw); irrigation with polluted water for ... | 2007 | 17365313 |
| the rice yabby1 gene is involved in the feedback regulation of gibberellin metabolism. | gibberellin (ga) biosynthesis is regulated by feedback control providing a mechanism for ga homeostasis in plants. however, regulatory elements involved in the feedback control are not known. in this report, we show that a rice (oryza sativa) yabby1 (yab1) gene had a similar expression pattern as key rice ga biosynthetic genes ga3ox2 and ga20ox2. overexpression of yab1 in transgenic rice resulted in a semidwarf phenotype that could be fully rescued by applied ga. quantification of the endogenous ... | 2007 | 17369428 |
| transcript profiling of the anoxic rice coleoptile. | rice (oryza sativa) seeds can germinate in the complete absence of oxygen. under anoxia, the rice coleoptile elongates, reaching a length greater than that of the aerobic one. in this article, we compared and investigated the transcriptome of rice coleoptiles grown under aerobic and anaerobic conditions. the results allow drawing a detailed picture of the modulation of the transcripts involved in anaerobic carbohydrate metabolism, suggesting up-regulation of the steps required to produce and met ... | 2007 | 17369434 |
| role of the c-terminal domains of rice (oryza sativa l.) bzip proteins rf2a and rf2b in regulating transcription. | rice (oryza sativa l.) transcription factors rf2a and rf2b are bzip (basic leucine zipper) proteins that interact with, and activate transcription from the rtbv (rice tungro bacilliform virus) promoter. here we characterize the c-terminal domains of rf2a and rf2b: these domains are rich in glutamine and proline/glutamine, respectively. affinity pull-down assays demonstrated that the c-terminal domains of rf2a and rf2b can associate to form either homodimers or heterodimers; however, they do not ... | 2007 | 17371296 |
| global identification and characterization of transcriptionally active regions in the rice genome. | genome tiling microarray studies have consistently documented rich transcriptional activity beyond the annotated genes. however, systematic characterization and transcriptional profiling of the putative novel transcripts on the genome scale are still lacking. we report here the identification of 25,352 and 27,744 transcriptionally active regions (tars) not encoded by annotated exons in the rice (oryza. sativa) subspecies japonica and indica, respectively. the non-exonic tars account for approxim ... | 2007 | 17372628 |
| analysis of the barley chromosome 2 region containing the six-rowed spike gene vrs1 reveals a breakdown of rice-barley micro collinearity by a transposition. | in cultivated barley (hordeum vulgare ssp. vulgare), six-rowed spikes produce three times as many seeds per spike as do two-rowed spikes. the determinant of this trait is the mendelian gene vrs1, located on chromosome 2h, which is syntenous with rice (oryza sativa) chromosomes 4 and 7. we exploited barley-rice micro-synteny to increase marker density in the vrs1 region as a prelude to its map-based cloning. the rice genomic sequence, covering a 980 kb contig, identified barley ests linked to vrs ... | 2007 | 17375281 |
| characterization of a lipoate-protein ligase a gene of rice (oryza sativa l.). | lipoic acid is an essential disulfide cofactor required for the lipoate-dependent enzymes including pyruvate dehydrogenase (pdh), alpha-ketoglutarate dehydrogenase (kgdh), and glycine cleavage enzymes that function in key metabolic pathways in most prokaryotes and eukaryotes. lipoic acid is covalently bound to lipoate-dependent enzymes by lipoate-protein ligase or lipoate transferase. here, we characterized a lipoyl-protein ligase a (oslpla) gene of rice. the oslpla gene, which encoded 270 amino ... | 2007 | 17376611 |
| increased rubisco content in transgenic rice transformed with the 'sense' rbcs gene. | rice (oryza sativa l.) plants with substantially increased rubisco content were obtained by agrobacterium-mediated transformation with the rice rbcs sense gene under the control of the rice rbcs promoter. the primary transformants were screened for the ratio of rubisco to leaf-n content, and the transformants with >120% wild-type levels of rubisco were selected. in the progeny of the selected lines of the transformants, the mrna levels of one member of the rbcs gene family were increased from 3. ... | 2007 | 17379698 |
| molecular cloning and characterization of a novel snap25-type protein gene ossnap32 in rice (oryza sativa l.). | the snap25-type proteins belong to the superfamily of the snares (soluble n-ethylmaleimide-sensitive factor attachment protein receptors), and function as important components of the vesical trafficking machinery in eukaryotic cells. in this paper, we report the cloning and expression characterization of ossnap32 gene, and the subcellular localization of its encoded protein. the ossnap32 gene contains five exons and four introns, and is located between rflp markers c12276s and s1917 on chromosom ... | 2008 | 17380428 |
| saturated humidity accelerates lateral root development in rice (oryza sativa l.) seedlings by increasing phloem-based auxin transport. | auxin transport plays a significant role modifying plant growth and development in response to environmental signals such as light and gravity. however, the effect of humidity on auxin transport is rarely documented. it is shown here that the transport of labelled indole-3-acetic acid (iaa) from the shoot to the root is accelerated in rice (oryza sativa l. ssp. indica cv. ir8) seedlings grown under saturated humidity (sh-seedlings) compared with plants grown under normal humidity (nh-seedlings). ... | 2007 | 17383991 |
| comprehensive expression profiling of rice grain filling-related genes under high temperature using dna microarray. | to elucidate the effect of high temperature on grain-filling metabolism, developing rice (oryza sativa) "nipponbare" caryopses were exposed to high temperature (33 degrees c/28 degrees c) or control temperature (25 degrees c/20 degrees c) during the milky stage. comprehensive gene screening by a 22-k dna microarray and differential hybridization, followed by expression analysis by semiquantitative reverse transcription-pcr, revealed that several starch synthesis-related genes, such as granule-bo ... | 2007 | 17384160 |
| a multiresidue method for the analysis of pesticide residues in polished rice (oryza sativa l.) using accelerated solvent extraction and gas chromatography and confirmation by mass spectrometry. | an analytical procedure using accelerated solvent extraction and gas chromatography with an electron capture detector has been optimized to simultaneously determine the residue of two insecticides (diazinon and epn) and one fungicide (isoprothiolane) in polished rice and was confirmed by gc-mass spectrometry. several parameters, including temperature, pressure, solvent ratio, cell size and cell cycle, were thoroughly investigated to find the optimal extraction conditions. the average recoveries ... | 2007 | 17385804 |
| proteome and phosphoproteome differential expression under salinity stress in rice (oryza sativa) roots. | salinity stress is a major abiotic stress that limits agriculture productivity worldwide. rice is a model plant of monocotyledons, including cereal crops. studies have suggested a critical role of protein phosphorylation in salt stress response in plants. however, the phosphoproteome in rice, particularly under salinity stress, has not been well studied. here, we use pro-q diamond phosphoprotein stain to study rice phosphoproteome differential expression under salt stress. seventeen differential ... | 2007 | 17385905 |
| enhancing salt tolerance in a crop plant by overexpression of glyoxalase ii. | earlier we have shown the role of glyoxalase overexpression in conferring salinity tolerance in transgenic tobacco. we now demonstrate the feasibility of same in a crop like rice through overproduction of glyoxalase ii. the rice glyoxalase ii was cloned in pcambia1304 and transformed into rice (oryza sativa cv pb1) via agrobacterium. the transgenic plants showed higher constitutive activity of glyoxalase ii that increased further upon salt stress, reflecting the upregulation of endogenous glyoxa ... | 2008 | 17387627 |
| dicot and monocot plants differ in retinoblastoma-related protein subfamilies. | the present study supports the view that the retinoblastoma functions are shared by two distinct retinoblastoma-related (rbr) protein subfamilies in the monocot cereal species, whereas dicot plants have only a single rbr protein. genes encoding rbr proteins were identified and characterized in alfalfa (medicago sativa), rice (oryza sativa), and wheat (triticum aestivum). the alfalfa msrbr gene encodes a new member of the dicot rbr proteins (subfamily a). a comparison was made of two rice genes, ... | 2007 | 17389586 |
| weed control in rice with metham-sodium. | metam-sodium is a soil fumigant with herbicidal properties. a field experiment was conducted in 2000 at copiano (pavia, italy) to determine the efficacy of three rates of metam-sodium (300, 450 and 600 l/ha) at three different planting times (5, 12 and 18 days after chemical treatments) for the control of weeds in rice cultivation. the study mainly focused on the control of red rice (oryza sativa var. selvatica), a weed which is worldwide distributed in rice fields and difficult to eradicate tes ... | 2006 | 17390826 |
| increase in cpd photolyase activity functions effectively to prevent growth inhibition caused by uvb radiation. | rice cultivars vary widely in their sensitivity to ultraviolet b (uvb) and this has been correlated with cyclobutane pyrimidine dimer (cpd) photolyase mutations that alter the structure/function of this photorepair enzyme. here, we tested whether cpd photolyase function determines the uvb sensitivity of rice (oryza sativa) by generating transgenic rice plants bearing the cpd photolyase gene of the uv-resistant rice cultivar sasanishiki in the sense orientation (s-b and s-c lines) or the antisens ... | 2007 | 17397507 |
| rice udp-glucose pyrophosphorylase1 is essential for pollen callose deposition and its cosuppression results in a new type of thermosensitive genic male sterility. | udp-glucose pyrophosphorylase (ugpase) catalyzes the reversible production of glucose-1-phosphate and utp to udp-glucose and pyrophosphate. the rice (oryza sativa) genome contains two homologous ugpase genes, ugp1 and ugp2. we report a functional characterization of rice ugp1, which is expressed throughout the plant, with highest expression in florets, especially in pollen during anther development. ugp1 silencing by rna interference or cosuppression results in male sterility. expressing a doubl ... | 2007 | 17400897 |
| induced expression of oryzain alpha gene encoding a cysteine proteinase under stress conditions. | oryzain alpha-a, a cysteine proteinase gene was cloned from rice (oryza sativa l. cv. aichi-asahi) leaves infected with magnaporthe grisea. the protein sequence deduced for oryzain alpha-a shares high identity with that of oryzain alpha, a gene expressed in germinating rice seed. oryzain alpha-a gene expression was induced by the blast fungus, magnaporthe grisea, and the transcript level was even higher in the compatible interaction with rice than in the incompatible interaction. expression of o ... | 2007 | 17404686 |
| the rice oslol2 gene encodes a zinc finger protein involved in rice growth and disease resistance. | arabidopsis lsd1-related proteins that contain lsd1-like zinc finger domains have been identified to be involved in disease resistance and programmed cell death. to investigate the potential role of lsd1-related gene in rice (oryza sativa l.), we cloned an lsd1 ortholog, oslol2, from the rice cdna plasmid library. the oslol2 gene is predicted to encode a polypeptide of 163 amino acids with two lsd1-like zinc finger domains with 74.5% identity to those of lsd1. southern blot analysis indicated th ... | 2007 | 17404758 |
| computational identification of novel pr-1-type genes in oryza sativa. | 2006 | 17406093 | |
| identification of the adp-glucose pyrophosphorylase isoforms essential for starch synthesis in the leaf and seed endosperm of rice (oryza sativa l.). | adp-glucose pyrophosphorylase (agp) catalyzes the first committed step of starch biosynthesis in higher plants. to identify agp isoforms essential for this biosynthetic process in sink and source tissues of rice plants, we analyzed the rice agp gene family which consists of two genes, osagps1 and osagps2, encoding small subunits (ssu) and four genes, osagpl1, osagpl2, osagpl3 and osagpl4, encoding large subunits (lsu) of this enzyme heterotetrameric complex. subcellular localization studies usin ... | 2007 | 17406793 |
| genome-wide analysis of the auxin response factors (arf) gene family in rice (oryza sativa). | auxin response factors (arfs) are transcription factors that bind with specificity to tgtctc-containing auxin response elements (auxres) found in promoters of primary/early auxin response genes and mediate responses to the plant hormone auxin. the arf genes are represented by a large multigene family in plants. a comprehensive genome-wide analysis was carried out in this study to find all arfs in arabidopsis (arabidopsis thaliana) and rice (oryza sativa subsp. japonica), 23 and 25 arf genes, nam ... | 2007 | 17408882 |
| the two-component signal system in rice (oryza sativa l.): a genome-wide study of cytokinin signal perception and transduction. | in this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (oryza sativa l.) genome sequence databases. thirty-seven genes were identified, including 5 hks (cytokinin-response histidine protein kinase) (oshk1-4, oshkl1), 5 hps (histidine phosphotransfer proteins) (oshp1-5), 15 type-a rrs (response regulators) (osrr1-15), 7 type b rr genes (osrr16-22), and 5 predicted pseudo-response regulators (osprr1-5). protein motif or ... | 2007 | 17408920 |
| divergent regulatory osmads2 functions control size, shape and differentiation of the highly derived rice floret second-whorl organ. | functional diversification of duplicated genes can contribute to the emergence of new organ morphologies. model eudicot plants like arabidopsis thaliana and antirrhinum majus have a single pi/glo gene that together with ap3/def regulate petal and stamen formation. lodicules of grass flowers are morphologically distinct reduced organs occupying the position of petals in other flowers. they serve a distinct function in partial and transient flower opening to allow stamen emergence and cross-pollin ... | 2007 | 17409064 |
| identification of the gravitropism-related rice gene lazy1 and elucidation of lazy1-dependent and -independent gravity signaling pathways. | we identified the gene responsible for three allelic lazy1 mutations of japonica rice (oryza sativa l.) by map-based cloning, complementation and rna interference. sequence analysis and database searches indicated that the wild-type gene (lazy1) encodes a novel and unique protein (lazy1) and that rice has no homologous gene. two lazy1 mutants were lazy1 null. confirming and advancing the previously reported results on lazy1 mutants, we found the following. (i) gravitropism is impaired, but only ... | 2007 | 17412736 |
| rice allelopathy induced by methyl jasmonate and methyl salicylate. | methyl jasmonate (meja) and methyl salicylate (mesa) are important signaling molecules that induce plant defense against insect herbivores and microbial pathogens. we tested the hypothesis that allelopathy is an inducible defense mechanism, and that the ja and sa signaling pathways may activate allelochemicals release. exogenous application of meja and mesa to rice (oryza sativa l.) enhanced rice allelopathic potential and led to accumulation of phenolics, an increase in enzymatic activities, an ... | 2007 | 17415624 |
| a phosphate starvation-induced acid phosphatase from oryza sativa: phosphate regulation and transgenic expression. | a phosphate starvation-induced acid phosphatase cdna was cloned from the rice, oryza sativa. the cdna encoding o. sativa acid phosphatase (osacp1) has 1100 bp with an open reading frame of 274 amino acid residues. the deduced amino acid sequence of osacp1 cdna showed 53% identity to tomato acid phosphatase and 46-50% identity to several other plant phosphatases. osacp1 expression was up-regulated in the rice plant and in cell culture in the absence of phosphate (pi). the induced expression of os ... | 2007 | 17415667 |
| rice non-yellow coloring1 is involved in light-harvesting complex ii and grana degradation during leaf senescence. | chlorophyll degradation is an aspect of leaf senescence, which is an active process to salvage nutrients from old tissues. non-yellow coloring1 (nyc1) is a rice (oryza sativa) stay-green mutant in which chlorophyll degradation during senescence is impaired. pigment analysis revealed that degradation of not only chlorophylls but also light-harvesting complex ii (lhcii)-bound carotenoids was repressed in nyc1, in which most lhcii isoforms were selectively retained during senescence. ultrastructura ... | 2007 | 17416733 |
| characterization of the rice circadian clock-associated pseudo-response regulators in arabidopsis thaliana. | members of the small family of arabidopsis pseudo-response regulators (prr1/toc1, prr3, prr5, prr7, and prr9) play roles close to the circadian clock in arabidopsis thaliana. we have reported that the rice (oryza sativa) genome also encodes a set of prr counterparts (designated osprr1, osprr37, osprr59, osprr73, and osprr95 respectively). to gain new insight into the molecular functions of osprrs, we carried out genetic complementation analyses by introducing two representative rice genes, osprr ... | 2007 | 17420570 |
| isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. cv. br29 of bangladesh. | a total of 30 bacteria were isolated from the rhizoplane of rice cv. br29 cultivated in mymensingh, bangladesh and from the seedlings obtained from surface-sterilized seeds of br29. upon screening, 6 isolates showed varying levels of phosphate solubilizing activity in both agar plate and broth assays using national botanical research institute's phosphate medium. the bacterial isolates were identified based on their phenotypic and 16s rrna genes sequencing data as acinetobacter sp. br-12, klebsi ... | 2007 | 17425114 |
| a sequence related to rice pong transposable element displays transcriptional activation by in vitro culture and reveals somaclonal variations in maize. | miniature inverted-repeat transposable elements (mites) are nonautonomous elements that are abundant in plant genomes. the rice mite mping was shown to be mobilized by anther culture, and the associated transposon pong was shown to transpose actively in an oryza sativa 'indica' rice cell-culture line. we have identified 3 sequences in maize named zmtpapong-like 1, 2, and 3 that displayed homology with the transposase of pong. here, we show that these sequences are differentially expressed during ... | 2006 | 17426755 |
| responses of rice cultivars with different nitrogen use efficiency to partial nitrate nutrition. | there is increased evidence that partial nitrate (no3-) nutrition (pnn) improves growth of rice (oryza sativa), although the crop prefers ammonium (nh4+) to no3- nutrition. it is not known whether the response to no3- supply is related to nitrogen (n) use efficiency (nue) in rice cultivars. methods solution culture experiments were carried out to study the response of two rice cultivars, nanguang (high-nue) and elio (low-nue), to partial no3- supply in terms of dry weight, n accumulation, grain ... | 2007 | 17428833 |
| high temperature stress and spikelet fertility in rice (oryza sativa l.). | in future climates, greater heat tolerance at anthesis will be required in rice. the effect of high temperature at anthesis on spikelet fertility was studied on ir64 (lowland indica) and azucena (upland japonica) at 29.6 degrees c (control), 33.7 degrees c, and 36.2 degrees c tissue temperatures. the objectives of the study were to: (i) determine the effect of temperature on flowering pattern; (ii) examine the effect of time of day of spikelet anthesis relative to a high temperature episode on s ... | 2007 | 17431025 |
| natural and artificially induced genetic variability in crop and model plant species for plant systems biology. | the sequencing of plant genomes which was completed a few years ago for arabidopsis thaliana and oryza sativa is currently underway for numerous crop plants of commercial value such as maize, poplar, tomato grape or tobacco. in addition, hundreds of thousands of expressed sequence tags (ests) are publicly available that may well represent 40-60% of the genes present in plant genomes. despite its importance for life sciences, genome information is only an initial step towards understanding gene f ... | 2007 | 17432262 |
| study on the diversity of endophytic communities from rice (oryza sativa l.) and their antagonistic activities in vitro. | endophytic populations were isolated from 2400 segments of oryza sativa collected from bhadra river project area, southern india during december 2005 (winter) and april 2006 (summer). overall colonization rates from surface sterilized tissues were 40.3% in roots and 25.83% in leaves during winter season, 20.15% in roots and 8.66% in leaves during summer season. nineteen different fungal taxa, a streptomyces sp. and bacterial species were isolated. streptomyces sp., chaetomium globosum, penicilli ... | 2009 | 17433644 |
| a homologue of the defender against the apoptotic death gene (dad1 )in uv-exposed chlamydomonas cells is downregulated with the onset of programmed cell death. | we report here the isolation of a homologue of the potential anti-apoptotic gene, defender against apoptotic death (dad1 )from chlamydomonas reinhardtii cells.using polymerase chain reaction (pcr),we investigated its expression in the execution process of programmed cell death (pcd)in uv-c exposed dying c.reinhardtii cells.reverse- transcriptase (rt)-pcr showed that c.reinhardtii dad1 amplification was drastically reduced in uv-c exposed dying c.reinhardtii cells.we connect the downregulation of ... | 2007 | 17435318 |
| steroidal constituents of rice (rryza sativa) hulls with algicidal and herbicidal activity against blue-green algae and duckweed. | two new compounds, 14-methyl stigmast-9(11)-en-3alpha-ol-3beta-d-glucopyranoside (1) and cholest-11-en-3beta, 6beta, 7alpha, 22beta-tetraol-24-one-3beta-palmitoleate (2), along with the known compound beta-sitosteryl-3beta-d-glucopyranosyl-6'-linoleiate (3), were isolated from the methanolic extract of rice (oryza sativa) hulls. the structures of the two new compounds were elucidated using one- and two-dimensional nmr in combination with ir, ei/ms, fab/ms, hr-ei/ms and hr-fab/ms. in bioassays wi ... | 2007 | 17439014 |
| cgkb: an annotation knowledge base for cowpea (vigna unguiculata l.) methylation filtered genomic genespace sequences. | cowpea [vigna unguiculata (l.) walp.] is one of the most important food and forage legumes in the semi-arid tropics because of its ability to tolerate drought and grow on poor soils. it is cultivated mostly by poor farmers in developing countries, with 80% of production taking place in the dry savannah of tropical west and central africa. cowpea is largely an underexploited crop with relatively little genomic information available for use in applied plant breeding. the goal of the cowpea genomic ... | 2007 | 17445272 |
| barren inflorescence2 encodes a co-ortholog of the pinoid serine/threonine kinase and is required for organogenesis during inflorescence and vegetative development in maize. | organogenesis in plants is controlled by meristems. axillary meristems, which give rise to branches and flowers, play a critical role in plant architecture and reproduction. maize (zea mays) and rice (oryza sativa) have additional types of axillary meristems in the inflorescence compared to arabidopsis (arabidopsis thaliana) and thus provide an excellent model system to study axillary meristem initiation. previously, we characterized the barren inflorescence2 (bif2) mutant in maize and showed th ... | 2007 | 17449648 |
| gene targeting by homologous recombination as a biotechnological tool for rice functional genomics. | the modification of an endogenous gene into a designed sequence by homologous recombination, termed gene targeting (gt), has broad implications for basic and applied research. rice (oryza sativa), with a sequenced genome of 389 mb, is one of the most important crops and a model plant for cereals, and the single-copy gene waxy on chromosome 6 has been modified with a frequency of 1% per surviving callus by gt using a strong positive-negative selection. because the strategy is independent of gene- ... | 0 | 17449652 |
| rubidium chloride tolerant callus cultures of rice (oryza sativa l.) accumulate more potassium and cross tolerate to other salts. | callus cultures from salt tolerant (csr-10) and susceptible (swarnadhan) varieties of oryza sativa l. were established in murashige and skoog's (ms) medium containing lethal concentrations (50 mm) of rubidium chloride (rbcl) as a selective agent. while 95-100% cells were viable in callus cultures grown without rbcl, viability was 75% in 50 mm rbcl selected cultures. growth of rbcl selected calli in presence of salt was comparable to that of callus grown without it. cells tolerant to rbcl showed ... | 2007 | 17453215 |
| overexpression of sbpase enhances photosynthesis against high temperature stress in transgenic rice plants. | activity of the calvin cycle enzyme sedoheptulose-1,7-bisphosphatase (sbpase) was increased by overexpression of a rice plants 9,311 (oryza sativa l.) cdna in rice plants zhonghua11 (oryza sativa l.). the genetic engineering enabled the plants to accumulate sbpase in chloroplasts and resulted in enhanced tolerance to high temperature stress during growth of young seedlings. moreover, co(2) assimilation of transgenic plants was significantly more tolerant to high temperature than that of wild-typ ... | 2007 | 17458549 |
| distribution of imidacloprid residues in different parts of rice plants and its effect on larvae and adult females of chilo suppressalis (lepidoptera: pyralidae). | the distribution of imidacloprid residue in different parts of rice, oryza sativa l. (graminales: poaceae), plants was investigated for three rice varieties. changes in larval hormones in chilo suppressalis (walker) (lepidoptera: pyralidae) that fed on imidacloprid-treated plant sources and the fecundity of resultant adult females (moths) also were determined. no significant differences in imidacloprid residue were detected in rice stem, leaves, and the unexpanded new leaf 7 d after treatment ex ... | 2007 | 17461061 |
| molecular and functional characterization of monocot-specific pex5p splicing variants, using ospex5pl and ospex5ps from rice (oryza sativa). | we identified two alternatively spliced variants of the peroxisomal targeting signal 1 (pts1) receptor protein pex5ps in monocot (rice, wheat, and barley) but not in dicot (arabidopsis and tobacco) plants. we characterized the molecular and functional differences between the rice (oryza sativa) pex5 splicing variants ospex5pl and ospex5ps. there is only a single-copy of ospex5 in the rice genome and rt-pcr analysis points to alternative splicing of the transcripts. putative light-responsive cis- ... | 2007 | 17464192 |
| comprehensive analysis of the expression of twenty-seven beta-1, 3-glucanase genes in rice (oryza sativa l.). | plant beta-1, 3-glucanases are involved in plant defense and in development. very little data are available on the expression of rice glucanases both in developmental tissues and under various stresses. in this study, we cloned and characterized twenty-seven rice beta-1, 3-glucanases (osglu) from at total of 71 putative glucanases. the osglu genes were obtained by pcr from a cdna library and were classified into seven groups (group i to vii) according to their dna or amino acid sequence homology ... | 2007 | 17464198 |
| compact genes are highly expressed in the moss physcomitrella patens. | compact genes contain short and few introns, and they are highly expressed in different animal genomes. recently, it has been shown that in oryza sativa and arabidopsis thaliana, highly expressed genes tend to be least compact, containing long and many introns. it has been suggested that selection on genome organization may have acted differently in plants compared with animals. gene expression can be estimated as the number of hits when comparing a gene sequence with publicly available expresse ... | 2007 | 17465932 |
| expression profiling of oryza sativa metal homeostasis genes in different rice cultivars using a cdna macroarray. | rice is an important food crop, but it is a poor source of essential micronutrients such as iron and zinc. in order to improve the metal ion content of rice grains through breeding or biotechnology, more information is needed on the molecular players that help mobilize metals from leaves to developing seeds. to profile several genes simultaneously, a cdna macroarray was developed using 36 metal-related genes from rice, including zips, nramps, and ysls (coding for known or potential metal transpo ... | 2007 | 17468002 |
| down-regulation of a silent information regulator2-related histone deacetylase gene, ossrt1, induces dna fragmentation and cell death in rice. | the silent information regulator2 (sir2) family proteins are nad(+)-dependent histone deacetylases. sir2 is involved in chromatin silencing at the mating-type loci, rdna, and telomeres in yeast and is associated with lifespan extension in yeast, worms, and flies, but also in a broader range of additional functions. in this work, we investigated the role of ossrt1, one of the two sir2-related genes found in rice (oryza sativa). we show that ossrt1 is a widely expressed nuclear protein with higher ... | 2007 | 17468215 |
| tilling mutants of lotus japonicus reveal that nitrogen assimilation and fixation can occur in the absence of nodule-enhanced sucrose synthase. | in all plant species studied to date, sucrose synthase occurs as multiple isoforms. the specific functions of the different isoforms are for the most part not clear. six isoforms of sucrose synthase have been identified in the model legume lotus japonicus, the same number as in arabidopsis (arabidopsis thaliana) and rice (oryza sativa). the genes encoding these isoforms are differentially expressed in all plant organs examined, although one, ljsus4, is only expressed in flowers. ljsus1 is the mo ... | 2007 | 17468221 |
| pea late bloomer1 is a gigantea ortholog with roles in photoperiodic flowering, deetiolation, and transcriptional regulation of circadian clock gene homologs. | genes controlling the transition to flowering have been studied in several species, including arabidopsis (arabidopsis thaliana) and rice (oryza sativa), but have not yet received much attention in legumes. here, we describe a new allelic series of late-flowering, photoperiod-insensitive mutants in the pea (pisum sativum) late bloomer1 (late1) gene and show that late1 is an ortholog of arabidopsis gigantea. mutants display defects in phytochrome b-dependent deetiolation under red light and in th ... | 2007 | 17468223 |
| lazy1 controls rice shoot gravitropism through regulating polar auxin transport. | tiller angle of rice (oryza sativa l.) is an important agronomic trait that contributes to grain production, and has long attracted attentions of breeders for achieving ideal plant architecture to improve grain yield. although enormous efforts have been made over the past decades to study mutants with extremely spreading or compact tillers, the molecular mechanism underlying the control of tiller angle of cereal crops remains unknown. here we report the cloning of the lazy1 (la1) gene that regul ... | 2007 | 17468779 |
| analysis of the conditional correlations from different genetic systems between the protein content and the appearance quality traits of indica rice. | a factorial mating design in two environments was conducted using 7 cytoplasmic male sterile lines (a) and 5 restorer lines (r) along with their f(1) (a x r) and f(2) populations. the unconditional and conditional analyses of genetic models and the corresponding statistic methods, including endospermic, cytoplasmic, and maternal plant genetic systems, were used to analyze the genetic relationships between protein content (pc) and the appearance quality traits of indica rice (oryza sativa l.). th ... | 2007 | 17469785 |
| chitinases in oryza sativa ssp. japonica and arabidopsis thaliana. | chitinases (ec3.2.1.14), found in a wide range of organisms, catalyze the hydrolysis of chitin and play a major role in defense mechanisms against fungal pathogens. the alignment and typical domains were analyzed using basic local alignment search tool (blast) and simple modular architecture research tool (smart), respectively. on the basis of the annotations of rice (oryza sativa l.) and arabidopsis genomic sequences and using the bio-software signalp3.0, tmhmm2.0, targetp1.1, and big-pi predic ... | 2007 | 17469786 |
| rnai knockdown of oryza sativa root meander curling gene led to altered root development and coiling which were mediated by jasmonic acid signalling in rice. | jasmonic acid (ja) is a well-known defence hormone, but its biological function and mechanism in rice root development are less understood. here, we describe a ja-induced putative receptor-like protein (osrlk, aal87185) functioning in root development in rice. rna in situ hybridization revealed that the gene was expressed largely in roots, and a fusion protein showed its localization on the plasma membrane. the primary roots in rnai transgenic rice plants meandered and curled more easily than wi ... | 2007 | 17470145 |
| gc-ei-tof-ms analysis of in vivo carbon-partitioning into soluble metabolite pools of higher plants by monitoring isotope dilution after 13co2 labelling. | the established gc-ei-tof-ms method for the profiling of soluble polar metabolites from plant tissue was employed for the kinetic metabolic phenotyping of higher plants. approximately 100 typical gc-ei-ms mass fragments of trimethylsilylated and methoxyaminated metabolite derivatives were structurally interpreted for mass isotopomer analysis, thus enabling the kinetic study of identified metabolites as well as the so-called functional group monitoring of yet non-identified metabolites. the monit ... | 2015 | 17475294 |
| developing a sustainable agro-system for central nepal using reduced tillage and straw mulching. | in nepal, soil erosion under maize (zea mays) agro-ecosystems is most critical during the pre-monsoon season. very few field experiments have been conducted on reduced tillage and rice straw (oryza sativa) mulching, although these conservation approaches have been recommended. thus, a five replicate field experiment was established in 2001 at kathmandu university (1500 m above sea level) on land with 18% slope to evaluate the efficiency of reduced tillage and mulching on soil and nutrient losses ... | 2008 | 17481804 |
| characterization of a set of phytochrome-interacting factor-like bhlh proteins in oryza sativa. | the model dicotyledon arabidopsis thaliana has a characteristic small sub-family of phytochrome-interacting bhlh (basic helix-loop-helix) factors, which are collectively designated the pil (or pif) (phytochrome interacting factor-like) family proteins. in this study, we identified and characterized a set of highly homologous members (designated ospil11 to ospil16) in the model monocotyledon rice (oryza sativa). some of them (ospil11, ospil12, and ospil13) showed the ability to interact with the ... | 2007 | 17485859 |
| identification and linkage mapping of complementary recessive genes causing hybrid breakdown in an intraspecific rice cross. | one outcome of hybrid breakdown is poor growth, which we observed as a reduction in the number of panicles per plant and in culm length in an f(2) population derived from a cross between the genetically divergent rice (oryza sativa l.) cultivars 'sasanishiki' (japonica) and 'habataki' (indica). quantitative trait locus (qtl) analysis of the two traits and two-way anova of the detected qtls suggested that the poor growth was due mainly to an epistatic interaction between genes at qtls located on ... | 2007 | 17486310 |
| a novel wx mutation caused by insertion of a retrotransposon-like sequence in a glutinous cultivar of rice (oryza sativa). | dna polymorphism of the wx gene in glutinous rice cultivars was investigated by pcr-rf-sscp and heteroduplex cleavage analysis using brassica petiole extract, and the nucleotide sequence variations were identified. most japonica-type glutinous rice was found to have a 23-bp duplication in the second exon, which causes loss of the function of granule-bound starch synthase (gbss) encoded by the wx gene. without the 23-bp duplication, there was an insertion of 7,764 bp in the ninth exon of the wx a ... | 2007 | 17492423 |
| irap and remap assessments of genetic similarity in rice. | rice is a model genome for cereal research, providing important information about genome structure and evolution. retrotransposons are common components of grass genomes, showing activity at transcription, translation and integration levels. their abundance and ability to transpose make them good potential markers. in this study, we used 2 multilocus pcr-based techniques that detect retrotransposon integration events in the genome: irap (inter-retrotransposon amplified polymorphism) and remap (r ... | 2007 | 17495343 |
| contrasting patterns in crop domestication and domestication rates: recent archaeobotanical insights from the old world. | archaeobotany, the study of plant remains from sites of ancient human activity, provides data for studying the initial evolution of domesticated plants. an important background to this is defining the domestication syndrome, those traits by which domesticated plants differ from wild relatives. these traits include features that have been selected under the conditions of cultivation. from archaeological remains the easiest traits to study are seed size and in cereal crops the loss of natural seed ... | 2007 | 17495986 |
| comparative cross-species alternative splicing in plants. | alternative splicing (as) can add significantly to genome complexity. plants are thought to exhibit less as than animals. an algorithm, based on expressed sequence tag (est) pairs gapped alignment, was developed that takes advantage of the relatively small intron and exon size in plants and directly compares pairs of ests to search for as. est pairs gapped alignment was first evaluated in arabidopsis (arabidopsis thaliana), rice (oryza sativa), and tomato (solanum lycopersicum) for which annotat ... | 2007 | 17496110 |
| genome-wide detection and analysis of alternative splicing for nucleotide binding site-leucine-rich repeats sequences in rice. | alternative splicing is a major contributor to genomic complexity and proteome diversity, yet the analysis of alternative splicing for the sequence containing nucleotide binding site and leucine-rich repeats (nbs-lrr) domain has not been explored in rice (oryza sativa l.). hidden markov model (hmm) searches were performed for nbs-lrr domain. 875 nbs-lrr-encoding sequences were obtained from the institute for genomic research (tigr). all of them were used to blast knowledge-based oryza molecular ... | 2007 | 17498622 |
| rice repetitive dna sequence rrd3: a plant promoter and its application to rna interference. | previously, a moderately repetitive dna sequence (rrd3) was cloned from rice (oryza sativa l.) by dna renaturation kinetics. sequence analysis revealed several conserved promoter motifs, including four tata-boxes and a caat-box, and promoter activity was shown in escherichia coli and mammalian expression systems. here, we inserted the rrd3 fragment into the plant promoter-capture vector, pcambia1391z, and examined whether the rrd3 fragment has promoter activity in plants. transgenic tobacco and ... | 2007 | 17498623 |
| physiological character and gene mapping in a new green- revertible albino mutant in rice. | a green-revertible albino mutant-qiufeng m was found from the japonica rice (oryza sativa l. ssp. japonica) qiufeng in the field. the first three leaves of the mutant were albino with some green. the leaf color became pale green since the fourth leaf and the glume had the same phenomenon as the first three leaves. the measuring data of the pigment content confirmed the visually observed results. it truly had a remarkable changing process in the leaf color in qiufeng m. comparison of the main agr ... | 2007 | 17498631 |
| progesterone: its occurrence in plants and involvement in plant growth. | progesterone is a mammalian gonadal hormone. in the current study, we identified and quantified progesterone in a range of higher plants by using gc-ms and examined its effects on the vegetative growth of plants. the growth of arabidopsis (arabidopsis thaliana) seedlings was promoted by progesterone at low concentrations but suppressed at higher concentrations under both light and dark growth conditions. the growth of the gibberellin-deficient mutant lh of pea (pisum sativum) was also promoted b ... | 2007 | 17512025 |
| the senescence-induced staygreen protein regulates chlorophyll degradation. | loss of green color in leaves results from chlorophyll (chl) degradation in chloroplasts, but little is known about how chl catabolism is regulated throughout leaf development. using the staygreen (sgr) mutant in rice (oryza sativa), which maintains greenness during leaf senescence, we identified sgr, a senescence-associated gene encoding a novel chloroplast protein. transgenic rice overexpressing sgr produces yellowish-brown leaves, and arabidopsis thaliana pheophorbide a oxygenase-impaired mut ... | 2007 | 17513504 |
| isolation and characterization of a novel cdna encoding erf/ap2-type transcription factor osap25 from oryza sativa l. | using a yeast one-hybrid method, a transcription factor, osap25, which interacts specifically with a gcc box was isolated from rice. the osap25 protein contained a conserved ethylene-responsive element binding factor (erf) domain which shared identity with other reported erf domains. phylogenetic analysis showed that osap25 could be categorized into class iii erf of the previously characterized erf proteins on an evolutionary relationship. the semi-quantitative rt-pcr analysis revealed that osap ... | 2007 | 17516027 |
| a cytosolic nadp-malic enzyme gene from rice (oryza sativa l.) confers salt tolerance in transgenic arabidopsis. | nadp-malic enzyme (nadp-me, ec 1.1.1.40) functions in many different pathways in plant and may be involved in plant defense such as wound and uv-b radiation. here, expression of the gene encoding cytosolic nadp-me (cytonadp-me, genbank accession no. ay444338) in rice (oryza sativa l.) seedlings was induced by salt stress (nacl). nadp-me activities in leaves and roots of rice also increased in response to nacl. transgenic arabidopsis plants over-expressing rice cytonadp-me had a greater salt tole ... | 2007 | 17516134 |
| a database of simple sequence repeats from cereal and legume expressed sequence tags mined in silico: survey and evaluation. | simple sequence repeats (ssrs) or microsatellites are an important class of molecular markers for genome analysis and plant breeding applications. in this paper, the ssr distributions within ests from the legumes soybean (glycine max, representing 135.86 mb), medicago (medicago truncatula, 121.1 mb) and lotus (lotus japonicus, 45.4 mb) have been studied relative to the distributions in cereals such as sorghum (sorghum bicolor, 98.9 mb), rice (oryza sativa, 143.9 mb) and maize (zea mays, 183.7 mb ... | 2006 | 17518768 |