Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| rhodococcus qingshengii sp. nov., a carbendazim-degrading bacterium. | a gram-positive, aerobic, non-motile, mesophilic strain, djl-6(t), able to degrade carbendazim, was isolated from a carbendazim-contaminated soil sample from jiangsu province, china. the taxonomic position of this isolate was analysed by using a polyphasic approach. chemotaxonomic analysis including peptidoglycan type, diagnostic sugar composition, fatty acid profile, menaquinones, polar lipids and mycolic acids showed that the characteristics of strain djl-6(t) were in good agreement with those ... | 2007 | 18048720 |
| biosynthesis of bile acids in a variety of marine bacterial taxa. | several marine bacterial strains, which were isolated from seawater off the island dokdo, korea, were screened to find new bioactive compounds such as antibiotics. among them, donghaeana dokdonensis strain dsw-6 was found to produce antibacterial agents, and the agents were then purified and analyzed by lc-ms/ms and 1d- and 2d-nmr spectrometries. the bioactive compounds were successfully identified as cholic acid and glycine-conjugated glycocholic acid, the 7alpha-dehydroxylated derivatives (deo ... | 2007 | 18050942 |
| safety and immunogenicity of a live-attenuated auxotrophic candidate vaccine against the intracellular pathogen rhodococcus equi. | rhodococcus equi causes serious pneumonia in neonatal foals and is an opportunistic pathogen of people with compromised cellular immunity. no effective vaccine against r. equi disease in foals is available. we tested the safety and immunogenicity of a live, fully attenuated riboflavin auxotrophic candidate vaccine strain of r. equi (r. equi rib-). we demonstrated that r. equi rib- is immunogenic and capable of inducing ifn-gamma responses in immunocompetent balb/c mice, yet it is safe even in an ... | 2008 | 18055071 |
| ocular pathogen or commensal: a pcr-based study of surface bacterial flora in normal and dry eyes. | to compare the bacterial population of the ocular surface of normal and dry eye subjects using conventional culture and 16s rdna pcr. | 2007 | 18055811 |
| construction of a recombinant plasmid as reaction control in routine pcr for detection of contagious equine metritis (cem-pcr). | contagious equine metritis (cem) is a highly contagious bacterial venereal disease of horses caused by taylorella equigenitalis. cem-pcr is a semi-nested pcr method for detecting this bacterium. although this technique is regarded as a sensitive diagnostic method for cem, there are risks of it generating false positive and false negative results. in this study, we constructed a recombinant plasmid (cem-pos) as reaction control to assure adequate pcr reaction and prevent false positive results ca ... | 2007 | 18057840 |
| [study on cooperating degradation of cypermethrin and 3-phenoxybenzoic acid by two bacteria strains]. | the microbial cooperated reaction is one of the most important forms of microbial degradation of organic pollutants. although there were many research reports of cooperating degradation, less report on the microbial cooperated of pyrethroid degradation to be found. we have isolated one degrading-bacteria strain named cdt3 for degradation of cypermethrin, which can degraded the cypermethrin into 3-pba and dcva. at the same time, we also isolated another degrading-bacteria strain named as pbm11, w ... | 2007 | 18062258 |
| [microbial diversity in scorpion intestine (buthus martensii karsch)]. | scorpion is an important officinal animal, and has a high nutritional value. in this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. results based on culture-independent method showed the bacteria to be related to alpha, beta, gamma-proteobacteria. bacteria isolated by the culture-dependent method were high g + c, gram-positive bacteria. the genera enterobacter, serratia and ochrobactrum were detected by b ... | 2007 | 18062268 |
| a mycolyl transferase mutant of rhodococcus equi lacking capsule integrity is fully virulent. | rhodococcus equi is a mucoid gram-positive facultative intracellular pathogen which can cause severe bronchopneumonia in foals and aids patients. a polysaccharide capsule which gives r. equi a mucoid appearance has long been suspected to be a virulence factor. here, we describe a transposome mutant in the gene fbpa of strain r. equi 103 causing absence of a capsular structure. fbpa is a chromosomal gene homologous to antigen 85 (ag85) mycolyl chain transferase gene of mycobacterium tuberculosis. ... | 2008 | 18063488 |
| deracemization of secondary alcohols through a concurrent tandem biocatalytic oxidation and reduction. | 2008 | 18072189 | |
| chromate reducing and plant growth promoting activities of psychrotrophic rhodococcus erythropolis mtcc 7,905. | a psychrotrophic bacterial strain resistant to 300 mg l(-1) of cr(6+) was isolated from metal contaminated soil samples from a site situated in the indian himalayan region. based on 16 s rrna analysis the isolate showed maximum similarity to rhodococcus erythropolis. rhodococcus erythropolis mtcc 7,905 reduced substantial amounts of cr(6+) to cr(3+) at 10 degrees c and showed plant growth promotion. the isolate offer promise as inoculant to promote plant growth of pea (pisum sativum) in the pres ... | 2007 | 18072252 |
| [pulmonary malakoplakia and rhodococcus equi infection. a report of two cases and review]. | malakoplakia is a rare, chronic, granulomatous disease seen in immunocompromised patients, caused by multiple infectious agents, most often bacteria. microscopically, accumulations of macrophages with granular cytoplasm and intra- or extracellular structures, called michaelis-gutmann bodies are characteristic. main cause of pulmonary malakoplakia is rhodococcus equi infection. we report two cases of pulmonary malakoplakia, in one case r. equi infection was confirmed. we also present a summary of ... | 2007 | 18080992 |
| purification and characterization of a dehydrogenase catalyzing conversion of n alpha-benzyloxycarbonyl-l-aminoadipic-delta-semialdehyde to n alpha-benzyloxycarbonyl-l-aminoadipic acid from rhodococcus sp. aiu z-35-1. | the enzyme catalyzing conversion of n alpha-benzyloxycarbonyl-l-aminoadipic-delta-semialdehyde (n alpha-z-l-aasa) to n alpha-benzyloxycarbonyl-l-aminoadipic acid (n alpha-z-l-aaa) in rhodococcus sp. aiu z-35-1 was identified, and its characteristics were revealed. this reaction was catalyzed by a dehydrogenase with a molecular mass of 59 kda. the dehydrogenase exhibited enzyme activity on not only n alpha-z-l-aasa but also n alpha-z-d-aasa and short chain aliphatic aldehydes, but not on aromatic ... | 2007 | 18086440 |
| mechanical properties of hexadecane-water interfaces with adsorbed hydrophobic bacteria. | this study focuses on how intact, hydrophobic bacteria in their stationary (i.e., non-dividing) phase could adsorb onto the hexadecane-water interface and alter its mechanical properties. the two strains of bacteria used in forming the interfacial films were acinetobacter venetianus rag-1 and rhodococcus erythropolis 20s-e1-c. using the dynamic pendant drop technique, the film interfacial tension was monitored as the surface area was made to undergo transient changes. under static conditions, bo ... | 2008 | 18093811 |
| the intracellular pathogen rhodococcus equi produces a catecholate siderophore required for saprophytic growth. | little is known about the iron acquisition systems of the soilborne facultative intracellular pathogen rhodococcus equi. we previously reported that expression of iupabc, encoding a putative siderophore abc transporter system, is iron regulated and required for growth at low iron concentrations. here we show that disruption of iupa leads to the concomitant accumulation of catecholates and a chromophore with absorption maxima at 341 and 528 nm during growth under iron-replete conditions. in contr ... | 2008 | 18156254 |
| soybean oil-degrading bacterial cultures as a potential for control of green peach aphids (myzus persicae). | microorganisms capable of degrading crude oil were isolated and grown in soybean oil as a sole carbon source. the microbial cultures were used to control green peach aphids in vitro. approximately 60% mortality of aphids was observed when the cultures were applied alone onto aphids. to examine the cultures as a pesticide formulation mixture, the cultures were combined with a low dose of the insecticide imidacloprid (one-fourth dose of recommended field-application rate) and applied onto aphids. ... | 2007 | 18156788 |
| genetic rearrangement strategy for optimizing the dibenzothiophene biodesulfurization pathway in rhodococcus erythropolis. | dibenzothiophene (dbt) and its derivatives can be microbially desulfurized by enzymes dszc, dsza, and dszb, which are encoded by the operon dszabc and contribute to the conversion in tandem. we investigated the expression characteristics of the dsz operon. our results revealed that the levels of transcription and translation of dsza, dszb, and dszc decreased according to the positions of the genes in the dsz operon. furthermore, the translation of dszb was repressed by an overlapping structure i ... | 2008 | 18165370 |
| [kinetics of the degradation of aliphatic hydrocarbons by the bacteria rhodococcus ruber and rhodococcus erythropolis]. | consumption of aliphatic hydrocarbons by the bacteria rhodococcus ruber ac-1513-d and rhodococcus erythropolis ac-1514-d grown on mixed n-alkanes and diesel fuel was studied. consumption of diesel fuel hydrocarbons by the strains was less intense in comparison with the n-alkane mixture. the strains showed differences in growth rate and consumption of the substrates, which suggests that they possess different mechanisms of hydrocarbon uptake. | 2007 | 18173107 |
| rhodostreptomycins, antibiotics biosynthesized following horizontal gene transfer from streptomyces padanus to rhodococcus fascians. | 2008 | 18179219 | |
| rhodococcus equi subcutaneous abscess in a patient with acquired immunodeficiency syndrome. | 2008 | 18181997 | |
| discovery and characterization of a putrescine oxidase from rhodococcus erythropolis ncimb 11540. | a gene encoding a putrescine oxidase (puorh, ec 1.4.3.10) was identified from the genome of rhodococcus erythropolis ncimb 11540. the gene was cloned in the pbad vector and overexpressed at high levels in escherichia coli. the purified enzyme was shown to be a soluble dimeric flavoprotein consisting of subunits of 50 kda and contains non-covalently bound flavin adenine dinucleotide as a cofactor. from all substrates, the highest catalytic efficiency was found with putrescine (km=8.2 microm, kcat ... | 2008 | 18183391 |
| modulation of the hormone setting by rhodococcus fascians results in ectopic knox activation in arabidopsis. | the biotrophic actinomycete rhodococcus fascians has a profound impact on plant development and a common aspect of the symptomatology is the deformation of infected leaves. in arabidopsis (arabidopsis thaliana), the serrated leaf margins formed upon infection resemble the leaf phenotype of transgenic plants with ectopic expression of knotted-like homeobox (knox) genes. through transcript profiling, we demonstrate that class-i knox genes are transcribed in symptomatic leaves. functional analysis ... | 2008 | 18184732 |
| a rhodococcus qsda-encoded enzyme defines a novel class of large-spectrum quorum-quenching lactonases. | a gene involved in n-acyl homoserine lactone (n-ahsl) degradation was identified by screening a genomic library of rhodococcus erythropolis strain w2. this gene, named qsda (for quorum-sensing signal degradation), encodes an n-ahsl lactonase unrelated to the two previously characterized n-ahsl-degrading enzymes, i.e., the lactonase aiia and the amidohydrolase aiid. qsda is related to phosphotriesterases and constitutes the reference of a novel class of n-ahsl degradation enzymes. it confers the ... | 2008 | 18192419 |
| production and structural elucidation of trehalose tetraesters (biosurfactants) from a novel alkanothrophic rhodococcus wratislaviensis strain. | to isolate a biosurfactant-producing bacterial strain and to identify and characterize the chemical structure and properties of its biosurfactants. | 2008 | 18194255 |
| differential mrna stability of the vapaicd operon of the facultative intracellular pathogen rhodococcus equi. | the gene encoding virulence associated protein a (vapa) is clustered with three vapa homologues (vapicd) within the pathogenicity island of the virulence plasmid of rhodococcus equi. northern blot analysis showed a vapa transcript of c. 700 nucleotides (nt) suggesting that vapa is a monocistronic transcript. however, using the more sensitive rt-pcr, it was shown that vapa is cotranscribed with the downstream vapicd genes forming a 2.3-kb operon. this initial transcript is subsequently processed ... | 2008 | 18205809 |
| discovery of a bacterial gene cluster for catabolism of the plant hormone indole 3-acetic acid. | the isolation and annotation of an 8994-bp dna fragment from pseudomonas putida 1290, which conferred upon p. putida kt2440 the ability to utilize the plant hormone indole 3-acetic acid (iaa) as a sole source of carbon and energy, is described. this iac locus (for indole 3-acetic acid catabolism) was identified through analysis of a plasposon mutant of p. putida 1290 that was no longer able to grow on iaa or indole 3-acetaldehyde and was unable to protect radish roots from stunting by exogenousl ... | 2008 | 18205812 |
| rhodococcus equi bacteremia with lung abscess misdiagnosed as corynebacterium: a report of 2 cases. | 2007 | 18209924 | |
| binding of the major phasin, phap1, from ralstonia eutropha h16 to poly(3-hydroxybutyrate) granules. | the surface of polyhydroxybutyrate (phb) storage granules in bacteria is covered mainly by proteins referred to as phasins. the layer of phasins stabilizes the granules and prevents coalescence of separated granules in the cytoplasm and nonspecific binding of other proteins to the hydrophobic surfaces of the granules. phasin phap1(reu) is the major surface protein of phb granules in ralstonia eutropha h16 and occurs along with three homologues (phap2, phap3, and phap4) that have the capacity to ... | 2008 | 18223073 |
| rhodococcus equi infection after alemtuzumab therapy for t-cell prolymphocytic leukemia. | rhodococcus equi, mainly known from veterinary medicine as a pathogen in domestic animals, can also cause infections in immunocompromised humans, especially in those with defects in cellular immunity. alemtuzumab, an anti-cd52 monoclonal antibody, causes lymphocytopenia by eliminating cd52-positive cells. we report a patient in whom rhodococcus equi infection developed after alemtuzumab therapy. | 2007 | 18258054 |
| crystals of dhaa mutants from rhodococcus rhodochrous ncimb 13064 diffracted to ultrahigh resolution: crystallization and preliminary diffraction analysis. | the enzyme dhaa from rhodococcus rhodochrous ncimb 13064 belongs to the haloalkane dehalogenases, which catalyze the hydrolysis of haloalkanes to the corresponding alcohols. the haloalkane dehalogenase dhaa and its variants can be used to detoxify the industrial pollutant 1,2,3-trichloropropane (tcp). three mutants named dhaa04, dhaa14 and dhaa15 were constructed in order to study the importance of tunnels connecting the buried active site with the surrounding solvent to the enzymatic activity. ... | 2008 | 18259069 |
| stabilization of water-in-oil emulsion by rhodococcus opacus b-4 and its application to biotransformation. | rhodococcus opacus b-4, which has recently been isolated as an organic solvent-tolerant bacterium, stabilized water-in-oil (w/o) emulsions by inhibition of droplet coalescence when the cells were dispersed in 90% (v/v) organic solvents. confocal microscopy revealed that many bacterial cells assembled at the interface between oil and water droplets, though free cells were also detectable at the inside of water droplets. bacterial cells in the w/o emulsion were capable of utilizing both a water-so ... | 2008 | 18270698 |
| assessment of microbial diversity in effluent treatment plants by culture dependent and culture independent approaches. | microbial community structure of two distinct effluent treatment plants (etps) of pesticide and pharmaceutical industries was assessed and defined by (i) culture dependent and culture independent approaches on the basis of 16s rrna gene sequencing, (ii) diversity index analysis - operational taxonomic units (otus). a total of 38 and 44 bacterial otus having 85-99% similarity with the closest match in the database were detected among pharmaceutical and pesticide sludge samples, respectively. fift ... | 2008 | 18280146 |
| phylogenetic analysis of long-chain hydrocarbon-degrading bacteria and evaluation of their hydrocarbon-degradation by the 2,6-dcpip assay. | thirty-six bacteria that degraded long-chain hydrocarbons were isolated from natural environments using long-chain hydrocarbons (waste car engine oil, base oil or the c-alkane fraction of base oil) as the sole carbon and energy source. a phylogenetic tree of the isolates constructed using their 16s rdna sequences revealed that the isolates were divided into six genera plus one family (acinetobacter, rhodococcus, gordonia, pseudomonas, ralstonia, bacillus and alcaligenaceae, respectively). furthe ... | 2008 | 18283542 |
| isolation, gene detection and solvent tolerance of benzene, toluene and xylene degrading bacteria from nearshore surface water and pacific ocean sediment. | btx (benzene, toluene and xylene) degrading bacteria were isolated from pacific ocean sediment and nearshore surface water. in the seawater near a ferry dock, degrading bacteria of a relatively wide diversity were detected, including species of pseudomonas, rhodococcus, exiguobacterium and bacillus; while species of bacillus only have been detected from the deep-sea sediment. most of the isolates showed degradation to more than one compound. generally better growth was obtained with p-xylene and ... | 2008 | 18286224 |
| characterization of cyclohexane and hexane degradation by rhodococcus sp. ec1. | cyclohexane is a recalcitrant compound that is more difficult to degrade than even n-alkanes or monoaromatic hydrocarbons. in this study, a cyclohexane-degrading consortium was obtained from oil-contaminated soil by an enrichment culture method. based on a 16s rdna polymerase chain reaction-denaturing gradient gel electrophoresis method, this consortium was identified as comprising alpha-proteobacteria, actinobacteria, and gamma-proteobacteria. one of these organisms, rhodococcus sp. ec1, was is ... | 2008 | 18289631 |
| isolation, identification and application of novel bacterial consortium tj-1 for the decolourization of structurally different azo dyes. | a novel bacterial consortium (tj-1), which could decolorize acid orange 7 (ao7) and manyother azo dyes, was developed. in tj-1 three bacterial strains were identified as aeromonas caviae, proteus mirabilis and rhodococcus globerulus by 16s rrna gene sequence analysis. ao7 decolorization was significantly higher with the use of consortium as compared to the use of individual strains, indicating complementary interactions among these strains. ao7 decolorization was observed under microaerophilic c ... | 2008 | 18289845 |
| aerobic biotransformation of decalin (decahydronaphthalene) by rhodococcus spp. | mixed bacterial cultures aerobically transformed decalin (decahydronaphthalene) dissolved in an immiscible carrier phase (heptamethylnonane; hmn) in liquid medium. conversion was enhanced in the presence of decane, a readily degraded n-alkane, and/or hmn. four rhodococcus spp. isolates purified from one of the mixed cultures were active against decalin in the presence of n-decane, but their ability to use decalin as a sole carbon source for growth could not be sustained. isolate iso 1a oxidized ... | 2008 | 18299805 |
| microbial biodegradation and toxicity of vinclozolin and its toxic metabolite 3,5-dichloroaniline. | vinclozolin, an endocrine disrupting chemical, is a chlorinated fungicide widely used to control fungal diseases. however, its metabolite 3,5-dichloroaniline is more toxic and persistent than the parent vinclozolin. for the biodegradation of vinclozolin, vinclozolin- and/or 3,5-dichloroaniline-degrading bacteria were isolated from pesticide-polluted agriculture soil. among the isolated bacteria, a rhodococcus sp. was identified from a 16s rdna sequence analysis and named rhodococcus sp. t1-1. th ... | 2008 | 18309282 |
| comparison of microbial and photochemical processes and their combination for degradation of 2-aminobenzothiazole. | the transformation of 2-aminobenzothiazole (abt) was studied under various conditions: (i) a photodegradation process at a lambda of >300 nm in the presence of an fe(iii)-nitrilotriacetic acid complex (fenta), (ii) a biodegradation process using rhodococcus rhodochrous obt18 cells, and (iii) the combined processes (fenta plus rhodococcus rhodochrous in the presence or absence of light). the transformation of abt in the combined system, with or without light, was much more efficient (99% degradat ... | 2008 | 18310409 |
| association of soil concentrations of rhodococcus equi and incidence of pneumonia attributable to rhodococcus equi in foals on farms in central kentucky. | to determine whether soil concentrations of total or virulent rhodococcus equi differed among breeding farms with and without foals with pneumonia caused by r equi. | 2008 | 18312138 |
| monitoring microbial populations on wide-body commercial passenger aircraft. | although exposure to bacteria has been assessed in cabin air previously, minimal numbers of samples have been collected in-flight. the purpose of this research was to comprehensively characterize bacterial concentrations in the aircraft cabin. twelve randomly selected flights were sampled on boeing-767 aircraft, each with a flight duration between 4.5 and 6.5 h. n-6 impactors were used to collect sequential, triplicate air samples in the front and rear of coach class during six sampling interval ... | 2008 | 18316352 |
| different bacterial strategies to degrade taurocholate. | aerobic enrichment cultures with taurocholate or alkanesulfonates as sole sources of carbon and energy for growth were successful and yielded nine bacterial isolates, all of which utilized taurocholate. growth was complex and involved not only many, usually transient, excretion products but also sorption of taurocholate and cholate to cells. three metabolic strategies to dissimilate taurocholate were elucidated, all of which involved bile salt hydrolase cleaving taurocholate to cholate and tauri ... | 2008 | 18320171 |
| detection of bphaa gene expression of rhodococcus sp. strain rha1 in soil using a new method of rna preparation from soil. | to understand the response of soil bacteria to the surrounding environment, it is necessary to examine the gene expression profiles of the bacteria in the soil. for this purpose, we developed a new method of extracting rna from soil reproducibly. using this new method, we extracted rna from a field soil, which was sterilized and inoculated with rhodococcus sp. strain rha1, a biphenyl degrader isolated from gamma-hexachlorocyclohexane-contaminated soil. data from agarose gel electrophoresis indic ... | 2008 | 18323657 |
| global response to desiccation stress in the soil actinomycete rhodococcus jostii rha1. | rhodococcus jostii rha1 is a soil-residing actinomycete with many favorable metabolic capabilities that make it an ideal candidate for the bioremediation of contaminated soils. arguably the most basic requirement for life is water, yet some nonsporulating bacteria, like rha1, can survive lengthy droughts. here we report the first transcriptomic analysis of a gram-positive bacterium during desiccation. filtered rha1 cells incubated at either low relative humidity (20%), as an air-drying treatment ... | 2008 | 18326668 |
| inhibitory effects of substrate and product on the carvone biotransformation activity of rhodococcus erythropolis. | the aqueous substrate and product toxicity thresholds in the microbial biotransformation of (-)-trans-carveol to the fragrance/flavor compound (r)-(-)-carvone by rhodococcus erythropolis were determined. above aqueous phase concentrations of approx. 500 mg carveol/l and 200-600 mg carvone/l, the biotransformation activity of the biocatalyst was inhibited. this biotransformation was undertaken in a single aqueous phase 3 l [corrected] reactor in which a total of 5 ml carveol (mixture of isomers) ... | 2008 | 18327538 |
| real-time pcr quantification of pah-ring hydroxylating dioxygenase (pah-rhdalpha) genes from gram positive and gram negative bacteria in soil and sediment samples. | real-time pcr based assays were developed to quantify gram positive (gp) and gram negative (gn) bacterial populations that are capable of degrading the polycyclic aromatic hydrocarbons (pah) in soil and sediment samples with contrasting contamination levels. these specific and sensitive real-time pcr assays were based on the quantification of the copy number of the gene that encodes the alpha subunit of the pah-ring hydroxylating dioxygenases (pah-rhdalpha), involved in the initial step of the a ... | 2008 | 18329116 |
| characterization of a cryptic plasmid from a greenland ice core arthrobacter isolate and construction of a shuttle vector that replicates in psychrophilic high g+c gram-positive recipients. | over 60 greenland glacial isolates were screened for plasmids and antibiotic resistance/sensitivity as the first step in establishing a genetic system. sequence analysis of a small, cryptic, 1,950 bp plasmid, p54, from isolate gic54, related to arthrobacter agilis, showed a region similar to that found in theta replicating rhodococcus plasmids. a 6,002 bp shuttle vector, psvj21, was constructed by ligating p54 and puc18 and inserting a chloramphenicol acetyl transferase (cat) cassette conferring ... | 2008 | 18335166 |
| construction of a genetically engineered and stable strain of degrading gamma-hexachlorocyclohexane and carbendazim by transposon mini-tn5. | the complete dehydrochlorinase gene lina of a gamma-hexachlorocyclohexane (gamma-hch) degrading strain sphingomonas sp. bhc-a, containing promoter and shine-dalgarno sequence (sd sequence), was amplified by pcr. the lina gene was inserted into noti-cut transposon vector put/mini-tn5 (km(r)) to get a novel transposon vector put/mini-tn5-lina. with the helper plasmid rk600, the transposon vector put/mini-tn5-lina was introduced into one carbendazim degrading gram-positive strain rhodococcus sp. dj ... | 2008 | 18338575 |
| differences of heterotrophic 13co2 assimilation by pseudomonas knackmussii strain b13 and rhodococcus opacus 1cp and potential impact on biomarker stable isotope probing. | motivated by the finding that pseudomonas knackmussii b13 but not rhodococcus opacus 1cp grows in the absence of externally provided co(2), we investigated the assimilation of (13)co(2) into active cells cultivated with non-labelled glucose as sole energy substrate. (13)c found in the bulk biomass indicated a substantial but different co(2) assimilation by pseudomonas and rhodococcus, respectively (3500 per thousand and 2600 per thousand). cellular fatty acids were labelled from -15 per thousand ... | 2008 | 18341583 |
| analysis of selective production of nalpha-benzyloxycarbonyl-l-aminoadipate-delta-semialdehyde and nalpha-benzyloxycarbonyl-l-aminoadipic acid by rhodococcus sp. aiu z-35-1. | the factors for selective production of n(alpha)-benzyloxycarbonyl-l-aminoadipate-delta-semialdehyde (n(alpha)-z-l-aasa) and n(alpha)-benzyloxycarbonyl-l-aminoadipic acid (n(alpha)-z-l-aaa) from n(alpha)-benzyloxycarbonyl-l-lysine (n(alpha)-z-l-lysine) by rhodococcus sp. aiu z-35-1 were analyzed. the cultivation time was important for selective production of n(alpha)-z-l-aasa, since n(alpha)-z-l-lysine oxidizing enzyme reached maximum at the early stage of cell growth and then decreased. the dif ... | 2008 | 18343343 |
| microbial degradation of 7-ketocholesterol. | 7-ketocholesterol (7kc) is an oxidized derivative of cholesterol suspected to be involved in the pathogenesis of atherosclerosis and possibly alzheimer's disease. while some oxysterols are important biological mediators, 7kc is generally cytotoxic and interferes with cellular homeostasis. despite recent interest in preventing the accumulation of 7kc in a variety of matrices to avoid adverse biological effects, its microbial degradation has not been previously addressed in the peer-reviewed liter ... | 2008 | 18344006 |
| atomic force microscopy measurement of heterogeneity in bacterial surface hydrophobicity. | the structure and physicochemical properties of microbial surfaces at the molecular level determine their adhesion to surfaces and interfaces. here, we report the use of atomic force microscopy (afm) to explore the morphology of soft, living cells in aqueous buffer, to map bacterial surface heterogeneities, and to directly correlate the results in the afm force-distance curves to the macroscopic properties of the microbial surfaces. the surfaces of two bacterial species, acinetobacter venetianus ... | 2008 | 18355095 |
| [effect of bacterial satellites on chlamydomonas reinhardtii growth in an algo-bacterial community]. | the growth characteristics of an algo-bacterial community (chlamydomonas reinhardtii and bacterial satellites) were studied, as well as the mechanism and patterns of bacterial effect on algae. four strains of predominant bacteria were isolated and partially characterized. they were assigned to the following taxa: rhodococcus terrea, micrococcus roseus, and bacillus spp. a pure culture of the alga under study was obtained by plating serial dilutions on agarized media with ampicillin. within the a ... | 2008 | 18365726 |
| phylogenetic and enzymatic diversity of deep subseafloor aerobic microorganisms in organics- and methane-rich sediments off shimokita peninsula. | "a meta-enzyme approach" is proposed as an ecological enzymatic method to explore the potential functions of microbial communities in extreme environments such as the deep marine subsurface. we evaluated a variety of extra-cellular enzyme activities of sediment slurries and isolates from a deep subseafloor sediment core. using the new deep-sea drilling vessel "chikyu", we obtained 365 m of core sediments that contained approximately 2% organic matter and considerable amounts of methane from offs ... | 2008 | 18368287 |
| chemoprophylactic effects of azithromycin against rhodococcus equi-induced pneumonia among foals at equine breeding farms with endemic infections. | to determine the effect of azithromycin chemoprophylaxis on the cumulative incidence of pneumonia caused by rhodococcus equi, age at onset of pneumonia, and minimum inhibitory concentration (mic) of azithromycin for r equi isolates cultured from fecal and clinical samples. | 2008 | 18380623 |
| r-stereoselective amidase from rhodococcus erythropolis no. 7 acting on 4-chloro-3-hydroxybutyramide. | ethyl (s)-4-chloro-3-hydroxybutyrate is an intermediate for the synthesis of atorvastatin, a chiral drug used for hypercholesterolemia. a rhodococcus erythropolis strain (no. 7) able to convert 4-chloro-3-hydroxybutyronitrile into 4-chloro-3-hydroxybutyric acid has recently been isolated from soil. this activity has been regarded as having been caused by the successive actions of the nitrile hydratase and amidase. in this instance, the corresponding amidase gene was cloned from the r. erythropol ... | 2008 | 18388476 |
| isolation and characterization of new cyclohexylacetic acid-degrading bacteria. | six cyclohexylacetic acid-degrading strains were isolated from soil samples in japan and identified as members of the genera cupriavidus (strain kua-1), rhodococcus, and dietzia by 16s rrna gene sequence analysis. for the first time members of these genera were shown to be capable of degrading cyclohexylacetic acid. a selected strain, kua-1, which is the first reported gram-negative organism capable of growth on cyclohexylacetic acid, was identified as a cupriavidus metallidurans, based on morph ... | 2008 | 18392890 |
| biofilm formation and partial biodegradation of polystyrene by the actinomycete rhodococcus ruber: biodegradation of polystyrene. | polystyrene, which is one of the most utilized thermoplastics, is highly durable and is considered to be non-biodegradable. hence, polystyrene waste accumulates in the environment posing an increasing ecological threat. in a previous study we have isolated a biofilm-producing strain (c208) of the actinomycete rhodococcus ruber that degraded polyethylene films. formation of biofilm, by c208, improved the biodegradation of polyethylene. consequently, the present study aimed at monitoring the kinet ... | 2008 | 18401686 |
| identification of functionally important amino acids in a novel indigo-producing oxygenase from rhodococcus sp. strain t104. | a novel indigo-producing oxygenase gene, designated ipoa (1,197 bp) was characterized from rhodococcus sp. strain t104. three indigo-negative mutations (a58v, p59l, and g251d) were obtained through random mutagenesis using an e. coli mutator strain. subsequent saturation mutagenesis resulted in the identification of nine and three amino acid substitutions that restore activity in the a58v and p59l mutants, respectively. activity was not restored in the g251d mutation by any other amino acids. in ... | 2008 | 18404265 |
| an amino acid at position 142 in nitrilase from rhodococcus rhodochrous atcc 33278 determines the substrate specificity for aliphatic and aromatic nitriles. | nitrilase from rhodococcus rhodochrous atcc 33278 hydrolyses both aliphatic and aromatic nitriles. replacing tyr-142 in the wild-type enzyme with the aromatic amino acid phenylalanine did not alter specificity for either substrate. however, the mutants containing non-polar aliphatic amino acids (alanine, valine and leucine) at position 142 were specific only for aromatic substrates such as benzonitrile, m-tolunitrile and 2-cyanopyridine, and not for aliphatic substrates. these results suggest th ... | 2008 | 18412544 |
| identification of alkane hydroxylase genes in rhodococcus sp. strain tmp2 that degrades a branched alkane. | rhodococcus sp. tmp2 is an alkane-degrading strain that can grow with a branched alkane as a sole carbon source. tmp2 degrades considerable amounts of pristane at 20 degrees c but not at 30 degrees c. in order to gain insights into microbial alkane degradation, we characterized one of the key enzymes for alkane degradation. tmp2 contains at least five genes for membrane-bound, non-heme iron, alkane hydroxylase, known as alkb (alkb1-5). phylogenetical analysis using bacterial alkb genes indicates ... | 2008 | 18414802 |
| preparation of homogenous oligosaccharide chains from glycosphingolipids. | after the discovery of glycosphingolipid (gsl) glycan detaching enzymes, rhodococcal endoglycoceramidase (egcase) and leech ceramide glycanase (cgase), the method for enzymatically releasing glycans from gsls has become the method of choice for preparing intact ceramide-free oligosaccharide chains from gsls. this paper describes (1) the preparation of the intact oligosaccharides from gm1 (ii(3)neuacggose(4)cer) and gbose(4)cer as examples to show the use of cgase to prepare intact glycan chains ... | 2009 | 18415015 |
| degradation of quinoline by rhodococcus sp. ql2 isolated from activated sludge. | a novel aerobic gram-positive bacterial strain capable of utilizing quinoline as sole source of carbon, nitrogen and energy was isolated from activated sludge of a coke plant wastewater treatment process. the isolate was identified as rhodococcus sp. ql2 based on its morphology, physiochemical properties in addition to the results from 16s rdna sequence analysis. the optimum temperature and the ph for its growth were 35-40 degrees c and 8.0, respectively. extra nitrogen sources stimulated the ba ... | 2008 | 18420344 |
| a novel lipopeptide produced by a pacific ocean deep-sea bacterium, rhodococcus sp. tw53. | our goal was to find a novel, biosurfactant-producing bacterium from pacific ocean deep-sea sediments. | 2008 | 18422956 |
| chimeric vapa/groel2 dna vaccines enhance clearance of rhodococcus equi in aerosol challenged c3h/he mice. | rhodococcus equi remains a significant bacterial pathogen, causing severe pyogranulomatous pneumonia in foals aged 1-3 months. there is no effective vaccine currently available for the prevention of r. equi pneumonia. dna vaccines are known to offer specific advantages over conventional vaccines. the aim of this study was to demonstrate efficacy of our recombinant dna vaccine candidates, namely pcdna3-re1, pcdna3-re3 and pcdna3-re5 by combining a heat shock protein groel2 to a virulence-associat ... | 2008 | 18423949 |
| two angular dioxygenases contribute to the metabolic versatility of dibenzofuran-degrading rhodococcus sp. strain ha01. | rhodococcus sp. strain ha01, isolated through its ability to utilize dibenzofuran (dbf) as the sole carbon and energy source, was also capable, albeit with low activity, of transforming dibenzo-p-dioxin (dd). this strain could also transform 3-chlorodibenzofuran (3cdbf), mainly by angular oxygenation at the ether bond-carrying carbon (the angular position) and an adjacent carbon atom, to 4-chlorosalicylate as the end product. similarly, 2-chlorodibenzofuran (2cdbf) was transformed to 5-chlorosal ... | 2008 | 18441103 |
| [isolation, identification and biodegradation characteristics of a bacterial strain able to degrade nonylphenol]. | a bacteria strain f-10 was isolated from activated carbon, which was processed with sand filtered water from songhua river for a long time, pre-oxidized with ozone, ppc and ferrate. the bacterial strain was capable of utilizing nonylphenol (np) as sole carbon source and identified as rhodococcus erythropolis with the sherlock microbial identification system (mis) from the midi corporation. the optimal conditions for np biodegradation in the shaking flasks were at 30 degrees c and ph 6.0. under t ... | 2008 | 18441946 |
| in vitro immunomodulating activity of biosurfactant glycolipid complex from rhodococcus ruber. | the biosurfactant glycolipid complex synthesized by rhodococcus ruber actinobacteria is not toxic and exhibits no appreciable effect on proliferative activity of peripheral blood leukocytes. in the monocyte fraction, the biosurfactant activates the production of il-1beta and tnf-alpha cytokines without modifying the production of il-6. in the mononuclear fraction, the glycolipid biosurfactant exhibited no effects on the production of il-1beta, tnf-alpha, and il-6. these results indicate good pro ... | 2007 | 18457028 |
| nitrile biotransformations for the synthesis of highly enantioenriched beta-hydroxy and beta-amino acid and amide derivatives: a general and simple but powerful and efficient benzyl protection strategy to increase enantioselectivity of the amidase. | biotransformations of a number of racemic beta-hydroxy and beta-amino nitrile derivatives were studied using rhodococcus erythropolis aj270, the nitrile hydratase and amidase-containing microbial whole cell catalyst, under very mild conditions. the overall enantioselectivity of nitrile biotransformations was governed predominantly by the amidase whose enantioselectivity was switched on remarkably by an o- and a n-benzyl protection group of the substrates. while biotransformations of beta-hydroxy ... | 2008 | 18459810 |
| a common soil flagellate (cercomonas sp.) grows slowly when feeding on the bacterium rhodococcus fascians in isolation, but does not discriminate against it in a mixed culture with sphingopyxis witflariensis. | flagellates are very important predators on bacteria in soil. because of their high growth rates, flagellate populations respond rapidly to changes in bacterial numbers. previous results indicate that actinobacteria are generally less suitable than proteobacteria as food for flagellates. in this study, we investigated the growth of the flagellate cercomonas sp. (atcc 50334) on each of the two bacteria sphingopyxis witflariensis (alphaproteobacteria) and rhodococcus fascians (actinobacteria) sepa ... | 2008 | 18462400 |
| defective production of interferon-gamma and tumour necrosis factor-alpha by aids mononuclear cells after in vitro exposure to rhodococcus equi. | the production of interferon-gamma and tumour necrosis factor-alpha was evaluated in the peripheral blood mononuclear cells (pbmcs) from healthy donors and aids patients after rhodococcus equi infection in vitro. pbmcs from healthy donors secreted elevated levels of ifn-gamma and tnf-alpha when challenged in vitro with killed r. equi, whereas the release of both cytokines was impaired in supernatant cultures from aids patients. we conclude that the failure of ifn-gamma generation in aids patient ... | 1995 | 18475656 |
| enhanced bioproduction of carvone in a two-liquid-phase partitioning bioreactor with a highly hydrophobic biocatalyst. | the microbial biotransformation of (-)-trans-carveol to the flavor and fragrance compound (r)-(-)-carvone by rhodococcus erythropolis dcl14 was carried out in a 3 l two phase partitioning bioreactor with an immiscible liquid second phase in an effort to improve upon the reactor performance achieved in a single aqueous phase system. the purpose of employing the liquid second phase is to minimize biotransformation rate inhibition due to the accumulation of the toxic substrate (cis-carveol) and pro ... | 2008 | 18478563 |
| applicability of the functional gene catechol 1,2-dioxygenase as a biomarker in the detection of btex-degrading rhodococcus species. | catechol 1,2-dioxygenase is a key enzyme in the degradation of monoaromatic pollutants. the detection of this gene is in focus today but recently designed degenerate primers are not always suitable. rhodococcus species are important members of the bacterial community involved in the degradation of aromatic contaminants and their specific detection could help assess functions and activities in the contaminated environments. to reach this aim, specific pcr primer sets were designed for the detecti ... | 2008 | 18479346 |
| rhodococcus cercidiphylli sp. nov., a new endophytic actinobacterium isolated from a cercidiphyllum japonicum leaf. | an endophytic actinobacterium, designated yim 65003(t), was isolated from a surface sterilized leaf sample of cercidiphyllum japonicum collected from yunnan province, south-west china. the morphological and chemotaxonomic properties of the isolate were typical of members of the genus rhodococcus. analysis of the 16s rrna gene sequence revealed that the isolate was most closely related to rhodococcus fascians dsm 20669(t) (99.6%) and rhodococcus yunnanensis yim 70056(t) (99.0%). dna-dna hybridiza ... | 2008 | 18485653 |
| the explosive-degrading cytochrome p450 system is highly conserved among strains of rhodococcus spp. | hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) is a widely used explosive and a serious environmental pollutant. nineteen strains of rhodococcus spp. capable of utilizing rdx as the sole nitrogen source have been isolated. the cytochrome p450 system xpla-xplb, which is responsible for rdx breakdown, is present in 18 of these strains. | 2008 | 18487400 |
| chemical composition and antimicrobial activity of the essential oils of the amazon guatteriopsis species. | the essential oils of guatteriopsis blepharophylla, guatteriopsis friesiana and guatteriopsis hispida were obtained by hydrodistillation and analysed by gc and gc/ms. the main compound found in the leaf oil of g. blepharophylla was caryophyllene oxide (1) (69.25%). the leaf oil of g. friesiana contained predominantly beta-eudesmol (2) (51.60%), gamma-eudesmol (3) (23.70%), and alpha-eudesmol (4) (14.56%). the major constituents identified in the leaf of g. hispida were beta-pinene (38.18%), alph ... | 2008 | 18495186 |
| isolation and characterisation of rhodococcus equi from submaxillary lymph nodes of wild boars (sus scrofa). | rhodococcus equi has been isolated from the submaxillary lymph nodes of domesticated pigs, but little is known about the presence of r. equi in wild boars. the aim of the study was the evaluation of the incidence of r. equi in wild boars and the characterisation of them. of 482 submaxillary lymph nodes of wild boars shot in 39 settlements throughout hungary, r. equi was isolated from 60 specimens, and plasmid types of 82 isolates were examined. the isolates were tested for the presence of 15-17- ... | 2008 | 18499361 |
| a pulmonary mass caused by rhodococcus equi infection in a renal transplant recipient. | a 62-year-old male kidney transplant recipient was admitted to hospital with a 14-day history of fever, hemoptysis and left-sided pleuritic chest pain. he had suffered malaise, weight loss, night sweats and exertional dyspnea over the previous 3 months. imaging studies of the patient's chest revealed a noncavitated mass measuring 5 x 8 cm in the anterior segment of the left upper lobe of the lung and a left-sided pleural effusion with septa, and bacterial cultures revealed the presence of rhodoc ... | 2008 | 18506169 |
| use of response surface optimization for the production of biosurfactant from rhodococcus spp. mtcc 2574. | the production of biosurfactant from rhodococcus spp. mtcc 2574 was effectively enhanced by response surface methodology (rsm). rhodococcus spp. mtcc 2574 was selected through screening of seven different rhodococcus strains. the preliminary screening experiments (one-factor at a time) suggested that carbon source: mannitol, nitrogen source: yeast extract and meat peptone and inducer: n-hexadecane are the critical medium components. the concentrations of these four media components were optimize ... | 2008 | 18511269 |
| improved biodesulfurization of hydrodesulfurized diesel oil using rhodococcus erythropolis and gordonia sp. | substituted benzothiophenes (bts) and dibenzothiophenes (dbts) remain in diesel oil following conventional desulfurization by hydrodesulfurization. a mixture of washed cells (13.6 g dry cell wt l(-1)) of rhodococcus erythropolis ds-3 and gordonia sp. c-6 were employed to desulfurize hydrodesulfurized diesel oil; its sulfur content was reduced from 1.26 g l(-1) to 180 mg l(-1), approx 86% (w/w) of the total sulfur was removed from diesel oil after three cycles of biodesulfurization. the average d ... | 2008 | 18516503 |
| selection and identification of bacterial strains with methyl-tert-butyl ether, ethyl-tert-butyl ether, and tert-amyl methyl ether degrading capacities. | nine bacterial strains isolated from two hydrocarbon-contaminated soils were selected because of their capacity for growth in culture media amended with 200 mg/l of one of the following gasoline oxygenates: methyl-tert-butyl ether (mtbe), ethyl-tert-butyl ether (etbe), and tert-amyl methyl ether (tame). these strains were identified by amplification of their 16s rrna gene, using fdl and rd1 primers, and were tested for their capacity to grow and biotransform these oxygenates in both mineral and ... | 2008 | 18522454 |
| rhodococcus kunmingensis sp. nov., an actinobacterium isolated from a rhizosphere soil. | a gram-positive, aerobic, non-motile actinobacterium strain, designated yim 45607(t), was isolated from a rhizosphere soil sample in kunming, south-west china. chemotaxonomically, the isolate contained chemical markers that supported its assignment to the genus rhodococcus. on the basis of 16s rrna gene sequence similarity analysis, strain yim 45607(t) formed a new subline within the genus rhodococcus, with rhodococcus equi as its closest phylogenetic neighbour (98.2 % 16s rrna gene sequence sim ... | 2008 | 18523196 |
| high benzene concentrations can favour gram-positive bacteria in groundwaters from a contaminated aquifer. | exposure to pollution exerts strong selective pressure on microbial communities, which may affect their potential to adapt to current or future environmental challenges. in this microcosm study, we used dna fingerprinting based on 16s rrna genes to document the impact of high concentrations of benzene on two bacterial communities from a benzene-contaminated aquifer situated below a petrochemical plant (siren, uk). the two groundwaters harboured distinct aerobic benzene-degrading communities able ... | 2008 | 18540887 |
| isolation of alkali-tolerant benzene-degrading bacteria from a contaminated aquifer. | to isolate benzene-degrading strains from neutral and alkaline groundwaters contaminated by benzene, toluene, ethylbenzene, xylenes (btex) from the siren aquifer, uk, and to test their effective ph range and ability to degrade tex. | 2008 | 18544140 |
| improved reactor performance and operability in the biotransformation of carveol to carvone using a solid-liquid two-phase partitioning bioreactor. | in an effort to improve reactor performance and process operability, the microbial biotransformation of (-)-trans-carveol to (r)-(-)-carvone by hydrophobic rhodococcus erythropolis dcl14 was carried out in a two phase partitioning bioreactor (tppb) with solid polymer beads acting as the partitioning phase. previous work had demonstrated that the substrate and product become inhibitory to the organism at elevated aqueous concentrations and the use of an immiscible second phase in the bioreactor w ... | 2008 | 18546419 |
| biosurfactant production by rhodococcus erythropolis grown on glycerol as sole carbon source. | the production of biosurfactant by rhodococcus erythropolis during the growth on glycerol was investigated. the process was carried out at 28 degrees c in a 1.5-l bioreactor using glycerol as carbon source. the bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. after 51 h of cultivation, 1.7 g/l of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mn/m, respectively, 67% of emulsifying index (e (24)), and ... | 2006 | 18563662 |
| rapid detection of microorganisms with nanoparticles and electron microscopy. | rapid detection of microorganisms is highly desirable. a procedure has been developed based on interactions between gold nanoparticles and proteins of microorganisms (escherichia coli, rhodococcus rhodochrous, and candida sp.) followed by scanning electron microscopy (sem). the nanoparticle-cell interaction was confirmed by ultraviolet resonance raman spectroscopy (uvrs) in the sem focus. cell suspensions in a buffer were interacted with gold nanoparticles (<10 nm in diameter) prepared from tetr ... | 2008 | 18570338 |
| effects of nicotinamide and riboflavin on the biodesulfurization activity of dibenzothiophene by rhodococcus erythropolis ustb-03. | rhodococcus erythropolis ustb-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene (dbt) via a sulfur-specific pathway in which dbt is converted to 2-hydroxybiphenyl (2hbp) as an end product. the effects of nicotinamide and riboflavin on the sulfur specific activity (sa) of dbt biodesulfurization by r. erythropolis ustb-03 were investigated. both nicotinamide and riboflavin were found to enhance the expression of sa, which was not previously reported. when r. erythro ... | 2008 | 18575116 |
| cloning and expression of the l-1-amino-2-propanol dehydrogenase gene from rhodococcus erythropolis, and its application to double chiral compound production. | the gene encoding nadp(+)-dependent l-1-amino-2-propanol dehydrogenase (aadh) of rhodococcus erythropolis mak154 was cloned and sequenced. a 780-bp nucleotide fragment was confirmed to be the gene encoding aadh by agreement of the n-terminal and internal amino acid sequences of the purified aadh. the gene (aadh) codes a total of 259 amino acid residues, and the deduced amino acid sequence shows similarity to several short-chain dehydrogenase/reductase family proteins. an expression vector, pkkaa ... | 2008 | 18584170 |
| purification, characterization, and substrate specificity of two 2,3-dihydroxybiphenyl 1,2-dioxygenase from rhodococcus sp. r04, showing their distinct stability at various temperature. | the genes of two 2,3-dihydroxybiphenyl 1,2-dioxygenases (bphc1 and bphc2) were obtained from the gene library of rhodococcus sp. r04. the enzymes have been purified to apparent electrophoretic homogeneity from the cell extracts of the recombinant harboring bphc1 and bphc2. both bphc1 and bphc2 were hexamers, consisting of six subunits of 35 and 33 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively. the enzymes had similar optimal ph (ph 9.0), but differe ... | 2008 | 18585431 |
| ultrasound enhanced bioprocesses: cholesterol oxidation by rhodococcus erythropolis. | cholesterol oxidation to cholestenone by resting cells of rhodococcus erythropolis atcc25544 was investigated under a computer-controlled ultrasonic irradiation at a frequency of 20 khz. the optimization of the ultrasound intensity and its mode of application to a stirred bioreactor was first established at a level which preserved the structural integrity of the cells and enabled their metabolic activity. a significant enhancement in the kinetic rates of the biotransformation was observed in mic ... | 1988 | 18587766 |
| epoxidation of propene by microbial cells immobilized on inorhanic supports. | immobilization of gas-utilizing microorganism strains (mycobacteria, rhodococcus, methane-utilizers) on inorganic supports based on alumina, silicates, and carbon was carried out to develop heterogeneous biocatalysts for the biotechnologic processes, including the process of propene epoxidation. adsorption ability of these microorganisms, biocatalytic properties of resting and immobilized bacterial cells, and effect of immobilization techniques on biocatalysis were studied. an approach of double ... | 1992 | 18600978 |
| biodesulfurization of water-soluble coal-derived material by rhodococcus rhodochrous igts8. | rhodococcus rhodochrous igts8 was previously isolated because of its ability to use coal as its sole source of sulfur for growth. subsequent growth studies have revealed that igts8 is capable of using a variety of organosulfur compounds as sources of sulfur but not carbon. in this article, the ability of igts8 to selectively remove organic sulfur from water-soluble coal-derived material is investigated. the microbial removal of organic sulfur from coal requires microorganisms capable of cleaving ... | 1992 | 18601220 |
| evolution of the rhodococcus equi vap pathogenicity island seen through comparison of host-associated vapa and vapb virulence plasmids. | the pathogenic actinomycete rhodococcus equi harbors different types of virulence plasmids associated with specific nonhuman hosts. we determined the complete dna sequence of a vapb(+) plasmid, typically associated with pig isolates, and compared it with that of the horse-specific vapa(+) plasmid type. pvapb1593, a circular 79,251-bp element, had the same housekeeping backbone as the vapa(+) plasmid but differed over an approximately 22-kb region. this variable region encompassed the vap pathoge ... | 2008 | 18606735 |
| [alkb homologues in thermophilic bacteria of the genus geobacillus]. | screening of alkane hydroxylase genes (alkb) was performed in the thermophilic aerobic bacteria of the genus geobacillus. total dna was extracted from the biomass of 11 strains grown on the mixture of saturated c10-c20 hydrocarbons, pcr amplification of fragments of alkb genes was performed with degenerate oligonucleotide primers, pcr products were cloned and sequenced. for the first time in the genome of thermophilic bacteria the presence of a set of alkb gene homologues was revealed. the strai ... | 2008 | 18610833 |
| respiratory infections in the immunocompromised patient. | damage to local and systemic defences of the lungs makes the immunocompromised host vulnerable to inhaled microorganisms. the type of underlying disease and its associated immunodeficiency allow a high degree of accurate pathogen prediction. neutropenia is associated with gram-negative bacilli pneumonia. prolonged neutropenia increases the risk of aspergillosis and mucormycosis. cellular immunodeficiency is associated with pneumocystis carinii, legionella spp. and intracellular parasites includi ... | 1993 | 18611585 |
| [isolation, identification and degradation characteristics of a quinoline-degrading bacterium rhodococcus sp ql2]. | a quinoline-degrading bacterium ql2, which utilizes quinoline as sole source of carbon, nitrogen and energy, was isolated from activated sludge in a coke-plant wastewater biological treatment system. according to the morphological characteristics, physiological and biochemical characteristics, and sequence analysis of 16s rrna, the strain was identified as rhodococcus sp.. the optimal temperature, initial ph, and shaker rotary speed for strain ql2 utilizing quinoline are 35-42 degrees c, ph 8-9, ... | 2008 | 18613525 |
| clinical and microbiological characteristics of community-acquired pneumonia among human immunodeficiency virus-infected patients in northern thailand. | human immunodeficiency virus (hiv) infections are prevalent in thailand. however, the clinical and microbiological characteristics of community-acquired pneumonia (cap) in such patients are not completely clear at present. in the present study, we analyzed the characteristics of cap in 191 hiv-infected patients (192 episodes, 130 males and 61 females, mean age 32.9 years, range: 20-62) who had been admitted to nakornping hospital in northern thailand between december 1996 and january 2002. the m ... | 2008 | 18622672 |
| enzyme engineering aspects of biocatalysis: cofactor regeneration as example. | reaction engineering is an important tool in the case of cofactor depending enzyme-catalyzed reactions. it allows the establishment of conditions resulting in lower product specific cofactor costs as compared with product-specific enzyme costs. this is shown for the stereospecific reduction of carbonyl compounds yielding chiral amino acids and alcohols. in continuous processes, cofactor costs can be reduced if the cofactor can be retained within the bioreactor or recycled into it after separatio ... | 1996 | 18629898 |
| biodegradation of paint stripper solvents in a modified gas lift loop bioreactor. | paint stripping wastes generated during the decontamination and decommissioning of former nuclear facilities contain paint stripping organics (dichloromethane, 2-propanol, and methanol) and bulk materials containing paint pigments. it is desirable to degrade the organic residues as part of an integrated chemical-biological treatment system. we have developed a modified gas lift loop bioreactor employing a defined consortium of rhodococcus rhodochrous strain ofs and hyphomicrobium sp. dm-2 that d ... | 1997 | 18636454 |
| detection and identification of bacteria intimately associated with fungi of the order sebacinales. | because of their beneficial impact on plants, the highly diverse mycorrhizal fungi grouped in the order sebacinales lay claim to high ecological and agricultural significance. here, we describe for the first time associations of sebacinoid members with bacteria. using quantitative pcr, denaturating gradient gel electrophoresis and fluorescence in situ hybridization, we detected an intimate association between piriformospora indica and rhizobium radiobacter, an alpha-proteobacterium. the stabilit ... | 2008 | 18637023 |