Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| flavaglines and triterpenoids from the leaves of aglaia forbesii. | three structurally complex flavaglines of the cyclopenta[bc]benzopyran type, named desacetylpyramidaglains a, c, d (1-3), and the triterpene 23, 24, 25-trihydroxycycloartan-3-one (4) were isolated from the leaves of aglaia forbesii together with the two rare pregnane steroids 2beta,3beta-dihydroxy-5alpha-pregn-17(z)-en-16-one and 2beta,3beta-dihydroxy-5alpha-pregn-17(e)-en-16-one, as well as the bisamide pyramidatine, the sesquiterpene spathulenol, and the widespread triterpenoids lupeol, lupeno ... | 2008 | 17707871 |
| il-18, but not il-12, regulates nk cell activity following intranasal herpes simplex virus type 1 infection. | infection of the respiratory tract with hsv type 1 (hsv-1) can have severe clinical complications, yet little is known of the immune mechanisms that control the replication and spread of hsv-1 in this site. the present study investigated the protective role of il-12 and il-18 in host defense against intranasal hsv-1 infection. both il-12 and il-18 were detected in lung fluids following intranasal infection of c57bl/6 (b6) mice. il-18-deficient (b6.il-18(-/-)) mice were more susceptible to hsv-1 ... | 2007 | 17709537 |
| dna polymerase mutations in drug-resistant herpes simplex virus mutants determine in vivo neurovirulence and drug-enzyme interactions. | mutations in the thymidine kinase and dna polymerase genes of herpes simplex virus (hsv) might confer resistance to antiviral drugs, particularly in immunocompromised patients who suffer from chronic and/or disseminated lesions. the patterns of cross-resistance and neurovirulence in mice of several dna polymerase mutants selected under pressure of foscarnet (pfa) and different acyclic nucleoside phosphonates (anps), including (s)-3-hydroxy-2-phosphonylmethoxypropyl (hpmp) derivatives of adenine ... | 2007 | 17713155 |
| effect of prophylactic oral acyclovir after penetrating keratoplasty for herpes simplex keratitis. | to determine the effect of routine use of prophylactic oral acyclovir after penetrating keratoplasty (pk) for herpes simplex virus (hsv) keratitis on recurrence, rejection, and graft failure rates. | 2007 | 17721290 |
| apolipoprotein e genotype influences vertical transmission of herpes simplex virus type 1 in a gender specific manner. | there is growing evidence that herpes simplex virus type 1 (hsv-1), together with the apolipoprotein e 4 (apoe4) allele, contribute to the pathogenesis of alzheimer's disease (ad), although the mechanism of their interaction remains uncertain. here we show that the combination of inherited apoe genotype and vertical transmission of hsv-1 confers a differential risk of brain infection. these risk factors are known to be associated with ad. | 2007 | 17725689 |
| hunting for ion channel modulators with herpes simplex virus. | 2007 | 17762875 | |
| role of 3-o-sulfated heparan sulfate in virus-induced polykaryocyte formation. | one way herpes simplex virus type-1 (hsv-1) spreads in vivo is by polykaryocytes formation. here we demonstrate that polykaryocyte production during hsv-1 spread in cultured human corneal fibroblasts (cf) required heparan sulfate (hs) and more specifically 3-o sulfated hs (3-os hs). the polykaryocyte formation heavily depended on the expression of hs on target (cf) cells but not on glycoprotein expressing effector cells. furthermore, we provide the first visual evidence of 3-os hs and hsv-1 gd c ... | 2007 | 17765228 |
| systemic delivery of (gamma1)34.5-deleted herpes simplex virus-1 selectively targets and treats distant human xenograft tumors that express high mek activity. | deltagamma(1)34.5 mutant herpes simplex type 1 viruses are under active clinical investigation as oncolytic therapy for cancer. mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (mek) activity has been shown to suppress protein kinase r and thereby confer oncolytic susceptibility to some human tumors by r3616, a virus deleted for both copies of gamma(1)34.5. we report that systemic delivery of r3616 can selectively target and destroy human xenograft tumors that overex ... | 2007 | 17804745 |
| lentiviral vector conferring resistance to mycophenolate mofetil and sensitivity to ganciclovir for in vivo t-cell selection. | several clinical studies of gene-modified t cells have shown limited in vivo function of the cells, immunogenicity of the transgene, and lack of a selective advantage for gene-modified t cells. to address these problems, we developed a lentiviral vector (lv) that provides a selectable, proliferative advantage and potentially decreases immunogenicity for transduced t cells. the bicistronic vector expressed two genes linked with an internal ribosomal entry site. one gene is a variant of the inosin ... | 2007 | 17805303 |
| susceptibility to hsv-1 infection and exercise stress in female mice: role of estrogen. | exhaustive exercise has been associated with an increased risk for upper respiratory tract infections in mice and humans. we have previously shown (brown as, davis jm, murphy ae, carmichael md, ghaffer a, mayer ep. med sci sports exerc 36: 1290-1295, 2004) that female mice are better protected from the lethal effects of herpes simplex virus type 1 (hsv-1) infection, both at rest and following exercise stress, but little is known about possible mechanisms. this study tested the effects of estroge ... | 2007 | 17823297 |
| development of an aciclovir implant for the effective long-term control of herpes simplex virus type-1 infection in vero cells and in experimentally infected skh-1 mice. | human herpes simplex virus type-1 (hsv-1) is treatable with oral doses of an antiviral agent such as aciclovir (acv), a drug that has poor bioavailability. an alternative for delivering acv would employ a long-lived subcutaneous implant that would allow for near zero-order drug delivery kinetics. this study aimed to develop an implant composed of a matrix of silicone and acv that is capable of sustained long-term release of acv. once the implants had been created, release of acv from the implant ... | 2007 | 17851051 |
| an oncolytic mutant of herpes simplex virus type-1 in which replication is governed by a promoter/enhancer of human papillomavirus type-16. | although herpes simplex virus type-1 (hsv-1) can be used as an oncolytic virus it has the undesirable side effect of neurotoxicity. to create a virus with improved specificity for oral cancer we used a fragment of human papillomavirus type-16, which is frequently found in oral and cervical cancers, but not elsewhere. the upstream regulatory region, urr16, was shown to have a high level of transcriptional activity in three of four oral cancer cell lines but low activity in three cell lines derive ... | 2007 | 17853922 |
| the varicella-zoster virus dna encapsidation genes: identification and characterization of the putative terminase subunits. | the putative dna encapsidation genes encoded by open reading frames (orfs) 25, 26, 30, 34, 43, 45/42 and 54 were cloned from varicella-zoster virus (vzv) strain ellen. sequencing revealed that the ellen orfs were highly conserved at the amino acid level when compared to those of 19 previously published vzv isolates. additionally, rt-pcr provided the first evidence that orf45/42 was expressed as a spliced transcript in vzv-infected cells. all seven orfs were expressed in vitro and full length pro ... | 2007 | 17868947 |
| socioeconomic gradients in immune response to latent infection. | there is a strong relation between socioeconomic position and health outcomes, although the mechanisms are poorly understood. the authors used data from 1,503 california participants in the 1998-1999 sacramento area latino study on aging aged 60-100 years to ask whether socioeconomic position is related to immune function as measured by the body's ability to keep latent herpesvirus antibody levels in a quiescent state. individuals with lower educational levels had significantly higher levels of ... | 2008 | 17873099 |
| an update on short-course episodic and prevention therapies for herpes genitalis. | the prevalence of herpes genitalis (genital herpes) has increased markedly over the past three decades. the most common cause is infection with the herpes simplex virus type 2 (hsv-2), but it can also occur as a result of hsv-1 infection. herpes genitalis can cause substantial psychosexual as well as physical morbidity and, in immunocompromised individuals, such as those who are hiv-positive, hsv infection can result in severe disease with progressive and extensive lesions. the natural history o ... | 2007 | 17877886 |
| divergence and recombination of clinical herpes simplex virus type 2 isolates. | herpes simplex virus type 2 (hsv-2) infects the genital mucosa and is one of the most common sexually transmitted viruses. here we sequenced a segment comprising 3.5% of the hsv-2 genome, including genes coding for glycoproteins g, i, and e, from 27 clinical isolates from tanzania, 10 isolates from norway, and 10 isolates from sweden. the sequence variation was low compared to that described for clinical hsv-1 isolates, with an overall similarity of 99.6% between the two most distant hsv-2 isola ... | 2007 | 17881457 |
| fold recognition insights into function of herpes icp4 protein. | icp4 is an important factor regulating the life cycle of hsv1. this conserved protein has several molecular functions, including activation of expression of viral late gene transcripts and inhibition of immediate early genes. although icp4 and its alphaherpesvirinae homologs (eg.: ie62 of vzv) have been subjects of various molecular studies, a complete view of their molecular function is lacking. here we present the results of fold recognition and molecular modelling of icp4 functional domains. ... | 2007 | 17882324 |
| rethinking herpes simplex virus infections in children and adolescents. | 2007 | 17889063 | |
| antiviral activity and mode of action of a peptide isolated from sorghum bicolor. | in this paper, we describe the purification of an antiviral peptide from seeds of sorghum bicolor l. by a procedure that included gel filtration, ion exchange, and high-performance liquid chromatography (hplc) in a reversed-phase column. its molecular weight, determined by chromatographic mobility on the shim-pack diol-150 gel permeation column in hplc, was found to be 2000da. the peptide designated 2kd peptide strongly inhibited the replication of herpes simplex virus type 1 (hsv-1), dose-depen ... | 2008 | 17890069 |
| systemic therapy of spontaneous prostate cancer in transgenic mice with oncolytic herpes simplex viruses. | oncolytic viruses are an innovative therapeutic strategy for cancer, wherein viral replication and cytotoxicity are selective for tumor cells. here we show the efficacy of systemically administered oncolytic viruses for the treatment of spontaneously arising tumors, specifically the use of oncolytic herpes simplex viruses (hsv) administered i.v. to treat spontaneously developing primary and metastatic prostate cancer in the transgenic tramp mouse, which recapitulates human prostate cancer progre ... | 2007 | 17909046 |
| risk factors of herpes simplex virus type 2 among std clinic attenders in delhi, india. | the present study was conducted to determine the seroprevalence and risk factors associated with hsv-2 infection among sexually transmitted diseases (std) clinic attenders of delhi in india. out of 128 patients included, 76 were males and 52 were females. antibodies to hsv 1 and 2 and hiv infection were determined by elisa. syphilis seropositivity was determined by vdrl test and confirm by tpha test. ulcer scrapping were stained by giemsa for herpes progenitalis and donovan bodies and grams for ... | 2006 | 17913210 |
| hydroxyurea enhances the activity of acyclovir and cidofovir against herpes simplex virus type 1 resistant strains harboring mutations in the thymidine kinase and/or the dna polymerase genes. | drug-resistant herpes simplex virus type 1 (hsv-1) recombinant strains harboring mutations in the thymidine kinase and/or the dna polymerase genes were evaluated for their susceptibility to various antivirals in the presence of 25 microg/ml of hydroxyurea (hyu). the latter compound decreased the 50% inhibitory concentrations of acyclovir by 1.5-3.8-fold and that of cidofovir by 2.7-14.4-fold. however, hyu did not affect the susceptibilities of the various recombinant mutants to foscarnet. hydrox ... | 2008 | 17913252 |
| temperature-sensitive mutations in the putative herpes simplex virus type 1 terminase subunits pul15 and pul33 preclude viral dna cleavage/packaging and interaction with pul28 at the nonpermissive temperature. | terminases comprise essential components of molecular motors required to package viral dna into capsids in a variety of dna virus systems. previous studies indicated that the herpes simplex virus type 1 u(l)15 protein (pu(l)15) interacts with the pu(l)28 moiety of a pu(l)28-pu(l)33 complex to form the likely viral terminase. in the current study, a novel temperature-sensitive mutant virus was shown to contain a mutation in u(l)33 codon 61 predicted to change threonine to proline. at the nonpermi ... | 2008 | 17913813 |
| purification and identification of antiviral components from laggera pterodonta by high-speed counter-current chromatography. | although laggera pterodonta, a folk medicine has been widely used for several centuries as an antiviral agent, there have been no studies to identify its antiviral components. a bioassay-guided phytochemical examination of l. pterodonta disclosed that its aqueous extract, which was made up of three dicaffeoylquinic acids showed significant inhibitory activity of virus replication. then a simple and efficient preparative high-speed counter-current chromatography (hsccc) method was successfully es ... | 2007 | 17920342 |
| rise in seroprevalence of herpes simplex virus type 1 among highly sexual active homosexual men and an increasing association between herpes simplex virus type 2 and hiv over time (1984-2003). | herpes simplex virus type 1 and type 2 (hsv-1 and hsv-2) are both highly prevalent. the rate of genital hsv-1 transmission is reportedly increasing over time. hsv-2 is considered to be an important risk factor for hiv transmission. we therefore studied changes in the hsv-1 and hsv-2 prevalence in a large cohort of men who have sex with men (msm) over a 20-year time period. | 2007 | 17929177 |
| inhibition of hsv-1 replication and hsv dna polymerase by the chloroxoquinolinic ribonucleoside 6-chloro-1,4-dihydro-4-oxo-1-(beta-d-ribofuranosyl) quinoline-3-carboxylic acid and its aglycone. | we describe in this paper that the synthetic chloroxoquinolinic ribonucleoside 6-chloro-1,4-dihydro-4-oxo-1-(beta-d-ribofuranosyl) quinoline-3-carboxylic acid (compound a) and its free aglycogene base (compound b) inhibit, with low cytotoxicity, the replication of herpes simplex virus type 1 and 2 (hsv-1 and hsv-2). compound a inhibited hsv-1 replication in vero cells with an ec(50) of 1.3 and 1.4 microm for an acyclovir (acv)-sensitive strain and an acv-resistant strain of this virus, respectiv ... | 2008 | 17931712 |
| biophysical investigation of human heparan sulfate d-glucosaminyl 3-o-sulfotransferase-3a: a mutual effect of enzyme oligomerisation and glycosaminoglycan ligand binding. | 3-o-sulfation of heparan sulfate (hs) is the rarest modification within heparan sulfate biosynthesis resulting in unique biological activities. heparan sulfate d-glucosaminyl 3-o-sulfotransferase-3a (3-ost-3a) (ec 2.8.2.23) generates a binding site for the envelope glycoprotein d (gd) of herpes simplex virus 1. we have expressed the sulfotransferase domain of the human heparan sulfate 3-ost-3a isoform in escherichia coli and subsequently purified the active enzyme which was found to be present a ... | 2007 | 17936096 |
| direct interaction between the n- and c-terminal portions of the herpes simplex virus type 1 origin binding protein ul9 implies the formation of a head-to-tail dimer. | ul9, a superfamily ii helicase, is a multifunctional protein required for herpes simplex virus type 1 replication in vivo. although the c-terminal 317-amino-acid dna binding domain of ul9 exists as a monomer, the full-length protein behaves as a dimer in solution. thus, it has been assumed that the n-terminal 534 residues contain a region necessary for efficient dimerization and that ul9 dimers are in a head-to-head configuration. we recently showed, however, that residues in the n terminus coul ... | 2007 | 17942532 |
| human herpes simplex labialis. | humans are the natural host for eight of more than 80 known herpes viruses. infections with herpes simplex virus type 1 (hsv-1) are ubiquitous worldwide and highly transmissible. herpes simplex labialis (hsl) is the best-recognized recrudescent infection of the lips and perioral tissues caused by hsv-1. facial lesions of hsl may be unsightly, frequent outbreaks unpleasant, and the infection itself more severe locally and systemically in immunocompromised people. this article highlights the patho ... | 2007 | 17953630 |
| the impact of dental surgery on hsv-1 reactivation in the oral mucosa of seropositive patients. | herpes simplex virus type 1 (hsv-1) is a member of the human herpes virus family. most of the population (90%) has antibodies to hsv-1, and as many as 40% of these individuals may develop secondary herpes. shedding of hsv-1 in the oral mucosa can be induced by many factors, including stress, sunlight, menstruation, and physical trauma. the aim of the present study was to evaluate the impact of dental surgical procedures on hsv-1 shedding in the oral mucosa. | 2007 | 17954324 |
| hiv-1/hsv-2 co-infected adults in early hiv-1 infection have elevated cd4+ t cell counts. | hiv-1 is often acquired in the presence of pre-existing co-infections, such as herpes simplex virus 2 (hsv-2). we examined the impact of hsv-2 status at the time of hiv-1 acquisition for its impact on subsequent clinical course, and total cd4+ t cell phenotypes. | 2007 | 17957262 |
| epidemiology of orofacial herpes simplex virus infections in the general population in france: results of the herpimax study. | prevalence of clinically manifest orofacial herpes in the general population is poorly characterized. objectives to establish the lifetime prevalence of clinically manifest orofacial herpes and its relationship with herpes simplex virus (hsv) serotype in the french general population. | 2007 | 17958848 |
| herpes simplex virus type 1 immediate-early protein icp27 is required for efficient incorporation of icp0 and icp4 into virions. | early in infection, herpes simplex virus type 1 (hsv-1) immediate-early (ie) proteins icp0 and icp4 localize to the nucleus, where they stimulate viral transcription. later in infection, icp0 and to a lesser extent icp4 accumulate in the cytoplasm, but their biological role there is unknown. previously, it was shown that the cytoplasmic localization of icp0/4 requires the multifunctional ie protein icp27, which is itself an activator of viral gene expression. here, we identify a viral icp27 muta ... | 2008 | 17959681 |
| synthesis and evaluation of cis-1-[4-(hydroxymethyl)-2-cyclopenten-1-yl]-5-[(124)i]iodouracil: a new potential pet imaging agent for hsv1-tk expression. | in our pursuit to find an appropriate reporter probe for herpes simplex virus type-1 thymidine kinase (hsv1-tk), a carbocyclic nucleoside analogue, cis-1-[4-(hydroxymethyl)-2-cyclopenten-1-yl]-5-[124i]iodouracil, has been efficiently synthesized. a pd(0)-catalyzed coupling reaction together with organotin and exchange reactions for radiolabeling gave more than 80% radiochemical yield with greater than 95% radiochemical purity and 1.15 gbq/mumol specific activity. biological data reveal that the ... | 2007 | 17960926 |
| role of af6 protein in cell-to-cell spread of herpes simplex virus 1. | af6 and its rat homologue afadin are multidomain proteins localized at cell junctions and involved in intercellular adhesion. af6 interacts via its pdz domain with nectin-1 at epithelial adherens junctions. nectin-1 serves as a mediator of cell-to-cell spread for herpes simplex virus 1 (hsv-1). we analyzed the role of af6 protein in the viral spread and nectin-1 clustering at cell-cell contacts by knockdown of af6 in epithelial cells. af6 knockdown reduced efficiency of hsv-1 spreading, however, ... | 2007 | 17967423 |
| three consecutive episodes of acute retinal necrosis due to herpes simplex-1 over twelve years following herpetic encephalitis. | to report a case of multiple delayed recurrence of acute retinal necrosis syndrome occurring in the same eye following initial herpetic encephalitis. | 2007 | 17972228 |
| herpes simplex virus type 1 preferentially targets human colon carcinoma: role of extracellular matrix. | viral therapy of cancer (viral oncolysis) is dependent on selective destruction of the tumor tissue compared with healthy tissues. several factors, including receptor expression, extracellular components, and intracellular mechanisms, may influence viral oncolysis. in the present work, we studied the potential oncolytic activity of herpes simplex virus type 1 (hsv-1), using an organ culture system derived from colon carcinoma and healthy colon tissues of mouse and human origin. hsv-1 infected no ... | 2008 | 17977977 |
| peripheral herpes simplex keratitis following lasik. | 2007 | 17985792 | |
| genetic susceptibility to herpes simplex virus 1 encephalitis in mice and humans. | herpes simplex encephalitis is a rare complication of herpes simplex virus 1 infection that strikes otherwise healthy individuals. its pathogenesis has long remained elusive. we highlight the investigations dealing with the genetic basis of herpes simplex encephalitis in mice and humans. | 2007 | 17989525 |
| a delicate balance: risk factors for acquisition of bacterial vaginosis include sexual activity, absence of hydrogen peroxide-producing lactobacilli, black race, and positive herpes simplex virus type 2 serology. | the etiology of bacterial vaginosis (bv) is poorly understood, but better definition of the risk factors associated with its acquisition should improve our understanding of this complex disease entity. | 2008 | 17989585 |
| antiviral activity of esterified alpha-lactalbumin and beta-lactoglobulin against herpes simplex virus type 1. comparison with the effect of acyclovir and l-polylysines. | the antiviral activity of methylated alpha-lactalbumin (met-ala), methylated and ethylated beta-lactoglobulins (met- and et-blg) was evaluated against acyclovir (acv)-sensitive and -resistant strains of herpes simplex virus type 1 (hsv-1) and compared to that of acv and l-polylysines (4-15 kda) using fixed or suspended vero cell lines. esterified whey proteins and their peptic hydrolyzates displayed protective action against hsv-1, which was relatively lower than that induced by acv or l-polylys ... | 2007 | 17990848 |
| the prevalence of human herpesvirus 6 in human sensory ganglia and its co-occurrence with alpha-herpesviruses. | human herpesvirus 6 (hhv-6) persists in the central nervous system, but its prevalence in the peripheral nervous system, a preferred latency site for herpesviruses, has not been studied. using nested polymerase chain reaction (pcr), the authors determined the distribution of hhv-6 in human sensory ganglia. hhv-6 was present in 30% of trigeminal, 40% of geniculate, 25% of vestibular, and 55% of dorsal root ganglia. it co-occurred with alpha-herpesviruses (herpes simplex virus type 1 or varicella- ... | 2007 | 17994431 |
| il-17 receptor signaling influences virus-induced corneal inflammation. | il-17 has been associated with selected inflammatory and autoimmune diseases. we characterized the expression of this proinflammatory cytokine following hsv-1 corneal infection and investigated whether il-17r signaling modulated the host response to the viral pathogen at early time-points postinfection. il-17 was elevated in the murine cornea 24 h after high-dose virus infection and subsequently persisted at low levels during the first week. immunofluorescent studies showed that the il-17r was e ... | 2008 | 17998300 |
| targeted integration of functional human atm cdna into genome mediated by hsv/aav hybrid amplicon vector. | ataxia-telangiectasia (a-t) is an autosomal recessive disorder characterized by neurodegeneration, immunodeficiency, cancer predisposition, genome instability, and sensitivity to ionizing radiation (ir). we have previously shown that a herpes simplex virus type 1 (hsv-1) amplicon vector carrying the human ataxia-telangiectasia mutated (atm) complementary dna (cdna) is able to correct aspects of the cellular phenotype of human a-t cells in culture, and is also able to transfer the atm cdna to the ... | 2008 | 17998902 |
| antiviral activity of some tunisian medicinal plants against herpes simplex virus type 1. | fifteen species of tunisian traditional medicinal plants, belonging to 10 families, were selected for this study. they were inula viscosa (l.) ait and reichardia tingitana (l.) roth ssp. discolor (pom.) batt. (asteraceae), mesembryanthemum cristallinum l. and m. nodiflorum l. (aizoaceae), arthrocnemum indicum (willd.) moq., atriplex inflata muell., a. parvifolia lowe var. ifiniensis (caball) maire, and salicornia fruticosa l. (chenopodiaceae), cistus monspeliensis l. (cistaceae), juniperus phoen ... | 2008 | 17999339 |
| xenophagy in herpes simplex virus replication and pathogenesis. | autophagy functions in part as an important host defense mechanism to engulf and degrade intracellular pathogens, a process that has been termed xenophagy. xenophagy is detrimental to the invading microbe in terms of replication and pathogenesis and many pathogens either dampen the autophagic response, or utilize the pathway to enhance their life cycle. herpes simplex virus type 1 (hsv-1) counteracts the induction of xenophagy through its neurovirulence protein, icp34.5. icp34.5 binds protein ph ... | 2008 | 18000391 |
| rapid, sensitive, and specific lateral-flow immunochromatographic point-of-care device for detection of herpes simplex virus type 2-specific immunoglobulin g antibodies in serum and whole blood. | herpes simplex virus type 2 (hsv-2) is a common human pathogen that can cause a variety of clinical manifestations in humans. in order to provide near-patient results to allow for faster counseling and treatment, a rapid point-of-care test that is accurate and simple to use is desirable. here, we describe the development and evaluation of an hsv-2 immunoglobulin g (igg)-specific antibody lateral-flow immunochromatographic assay (lfia) based on colloidal gold nanoparticles. a total of 359 serum s ... | 2008 | 18003814 |
| normal brain cells contribute to the bystander effect in suicide gene therapy of malignant glioma. | lentiviral vectors pseudotyped with glycoproteins of the lymphocytic choriomeningitis virus (lcmv-gp) are promising candidates for gene therapy of malignant glioma, as they specifically and efficiently transduce glioma cells in vitro and in vivo. here, we evaluated the therapeutic efficacy of lcmv-gp and vesicular stomatitis virus glycoprotein (vsv-g) pseudotyped vectors. | 2007 | 18006778 |
| effect of genital ulcer disease on hiv-1 coreceptor expression in the female genital tract. | to examine the expression of human immunodeficiency virus type 1 (hiv-1) coreceptors (ccr5 and cxcr4) by monocytic cells within human genital ulcers. | 2007 | 18008231 |
| sln as a topical delivery system for artemisia arborescens essential oil: in vitro antiviral activity and skin permeation study. | the effect of sln incorporation on transdermal delivery and in vitro antiherpetic activity of artemisia arborescens essential oil was investigated. two different sln formulations were prepared using the hot-pressure homogenization technique, compritol 888 ato as lipid, and poloxamer 188 and miranol ultra c32 as surfactants. formulations were examined for their stability for two years by monitoring average size distribution and zeta potential values. the antiviral activity of free and sln incorpo ... | 2007 | 18019840 |
| [radiograph patterns in herpes simples virus i pneumonia in the adult]. | the aim of this study is to describe epidemiology, patogénesis, pulmonary manifestations and rx findings in adult patients with varicella pneumonia (vp). four patients were studied. the diagnosis was established by clinical and radiologic criteria. all had fever , esanthem, 3 cough, 2 dyspnea. chest x-ray showed interstitial micronodular pattern at bases. 1 case developed airspace consolidation by staphylococcus aureus. 2 were admitted to icu. the four received iv acyclovir. we concluded that ad ... | 2007 | 18020880 |
| infection of human endothelium in vitro by cytomegalovirus causes enhanced expression of purinergic receptors: a potential virus escape mechanism? | human cytomegalovirus (cmv) uses different strategies to escape from human host defense reactions. previously we have observed that infection of endothelial cells with cmv in vitro leads to enhanced activity of endothelial ectonucleotidases. these ectoenzymes are responsible for hydrolysis of extracellular adenine nucleotides, resulting in the formation of adenosine. infection with cmv in vivo therefore may result in local increase of adenosine production, providing an anti-inflammatory and anti ... | 2007 | 18049120 |
| intercellular trafficking of vp22, a herpes simplex virus type 1 tegument protein. | the herpes simplex virus type 1 (hsv-1) tegument protein, vp22 has been reported to have the property of intercellular transport. the previous studies have shown that following expression of a fusion protein containing vp22; it spreads to every cell in a monolayer and concentrates in the nucleus. in spite of these reports, some studies have shown that vp22 trafficking and its nucleus accumulation is an artifact and no improvement in translocation of proteins fused to vp22 has been detected. | 2007 | 18051705 |
| translocation and colocalization of icp4 and icp0 in cells infected with herpes simplex virus 1 mutants lacking glycoprotein e, glycoprotein i, or the virion host shutoff product of the ul41 gene. | in wild-type herpes simplex virus 1-infected cells, the major regulatory protein icp4 resides in the nucleus whereas icp0 becomes dynamically associated with proteasomes and late in infection is translocated and dispersed in the cytoplasm. inhibition of proteasomal function results in retention or transport of icp0 to the nucleus. we report that in cells infected with mutants lacking glycoprotein e (ge), glycoprotein i (gi), or the product of the u(l)41 gene, both icp4 and icp0 are translocated ... | 2008 | 18057247 |
| antiviral and immunomodulatory effect of a lyophilized extract of capparis spinosa l. buds. | herpes simplex virus type 1 (hsv-1) and herpes simplex virus type 2 (hsv-2) are common human pathogens that in particular cases can also cause severe problems especially in immunodeficient patients. the present paper reports the antiviral and immunomodulatory properties of a methanolic extract of c. spinosa buds (cap), rich in flavonoids, including several quercetin and kaempferol glycosides. in particular we have investigated whether the in vitro exposure of human peripheral blood mononuclear c ... | 2008 | 18058988 |
| a conserved domain of herpes simplex virus icp34.5 regulates protein phosphatase complex in mammalian cells. | icp34.5, encoded by herpes simplex virus 1, is a protein phosphatase 1 (pp1) regulatory subunit that mediates dephosphorylation of the alpha subunit of translation initiation factor 2 (eif2alpha). however, the mechanism of its action remains poorly understood. here, we show that amino acid substitutions in the arginine-rich motif have differential effects on icp34.5 activity. the phenotypes parallel with viral protein synthesis and cytopathic effects in virus infected cells. besides the consensu ... | 2008 | 18068675 |
| terpenoids and phenolics from plectranthus strigosus, bioactivity screening. | further studies on plectranthus sp. (lamiaceae) led to the isolation of terpenes and phenol esters from plectranthus strigosus benth. ent-16-kauren-19-ol (1), ent-16-kauren-19-oic acid (2), xylopic acid (3), xylopinic acid (4), hinokiol (5), 4 beta,6 beta-dihydroxy-1 alpha,5 beta(h)-guai-9-ene (6) 4 beta,6 beta-dihydroxy-1 alpha,5 beta(h)-guai-10(14)-ene (7), mixtures of hexacosan-1,26-diol and octacosan-1,28-diol ferulate diesters (8), of esters from ferulic acid and fatty alcohols (9) and of e ... | 2008 | 18075900 |
| the essential human cytomegalovirus gene ul52 is required for cleavage-packaging of the viral genome. | replication of human cytomegalovirus (hcmv) produces large dna concatemers of head-to-tail-linked viral genomes that upon packaging into capsids are cut into unit-length genomes. the mechanisms underlying cleavage-packaging and the subsequent steps prior to nuclear egress of dna-filled capsids are incompletely understood. the hitherto uncharacterized product of the essential hcmv ul52 gene was proposed to participate in these processes. to investigate the function of pul52, we constructed a delt ... | 2008 | 18077717 |
| binding of icp4, tata-binding protein, and rna polymerase ii to herpes simplex virus type 1 immediate-early, early, and late promoters in virus-infected cells. | the binding of herpes simplex virus type 1 icp4, tata-binding protein (tbp), and rna polymerase ii (polii) to the promoter regions of representative immediate-early (ie) (icp0), early (e) (thymidine kinase [tk]), and late (l) (glycoprotein c [gc]) genes on the viral genome was examined as a function of time postinfection, viral dna replication, cis-acting sites for tfiid in the tk and gc promoters, and genetic background of icp4. the binding of tbp and polii to the ie icp0 promoter was independe ... | 2008 | 18094162 |
| nuclear egress and envelopment of herpes simplex virus capsids analyzed with dual-color fluorescence hsv1(17+). | to analyze the assembly of herpes simplex virus type 1 (hsv1) by triple-label fluorescence microscopy, we generated a bacterial artificial chromosome (bac) and inserted eukaryotic cre recombinase, as well as beta-galactosidase expression cassettes. when the bac phsv1(17(+))bluelox was transfected back into eukaryotic cells, the cre recombinase excised the bac sequences, which had been flanked with loxp sites, from the viral genome, leading to hsv1(17(+))bluelox. we then tagged the capsid protein ... | 2008 | 18160444 |
| c-reactive protein mediates the effect of apolipoprotein e on cytomegalovirus infection. | although the apolipoprotein (apoe)-epsilon4 allele has been shown to determine the outcome of several infections, its relationship with cytomegalovirus (cmv) has not been explored. we examine whether apoe determines cmv and herpes simplex virus type 1 (hsv-1) antibody levels and assess whether c-reactive protein (crp) mediates any observed relationships. | 2008 | 18171282 |
| characterization of mre11 loss following hsv-1 infection. | herpes simplex virus induces the activation of the cellular dna double strand break response pathway dependent upon initiation of viral dna replication. the mrn complex, consisting of mre11, rad50 and nbs1, is an essential component of the dna double strand break response and other reports have documented its presence at sites of viral dna replication, interaction with icp8 and its contribution to efficient viral dna replication. during our characterization of the dsb response following infectio ... | 2008 | 18177684 |
| dissecting the structural determinants of the interaction between the human cytomegalovirus ul18 protein and the cd85j immune receptor. | ul18 is a glycoprotein encoded by the human cytomegalovirus genome and is thought to play a pivotal role during human cytomegalovirus infection, although its exact function is still a matter of debate. ul18 shares structural similarity with mhc class i and binds the receptor cd85j on immune cells. besides ul18, cd85j binds mhc class i molecules. the binding properties of cd85j to mhc class i molecules have been thoroughly studied. conversely, very little information is available on the cd85j/ul1 ... | 2008 | 18178836 |
| in vivo evaluation of the uptake of [(123)i]fiau, [(123)i]ivfru and [(123)i]ivfau by normal mouse brain: potential for noninvasive assessment of hsv-1 thymidine kinase gene expression in gliomas. | radioiodinated 5-iodo-1-(2-fluoro-2-deoxy-beta-d-arabinofuranosyl)uracil (f *iau) is most commonly used for noninvasive assessment of herpes simplex virus type 1 thymidine kinase (hsv-1-tk) gene expression. however, it does not permeate the intact blood-brain barrier (bbb) because of its moderate lipophilicity. in this work, three iodo-nucleosides, fiau, ivfru, and ivfau, were radiolabeled with iodine-123 and tested for permeation of the bbb in mice and for potential measurement of hsv-1-tk gene ... | 2008 | 18188770 |
| evaluation of the t-rex transcription switch for conditional expression and regulation of hsv-1 vectors. | herpes simplex virus 1 (hsv-1) strain ang and angpath were cloned as bacterial artificial chromosome (bac). two different types of bac genomes were obtained. bac genomes of type i contained the bac replicon at the intended target region between the genes of ul48 and ul49. in bac genomes of type ii, the bac sequences were found to be aberrantly fused between the termini of the hsv-1 genome. both the bac types were used to establish a conditional gene expression system for hsv-1 by flp recombinase ... | 2008 | 18193347 |
| murine gammaherpesvirus 68 serum antibodies in general human population. | previous studies using elisa and virus neutralization test (vnt) have proved the presence of murine gammaherpesvirus 68 (mhv-68) serum antibodies in sera of laboratory staff working with mhv-68, as well as in the general population. in this study, we investigated the incidence of serum antibodies to mhv-68 and to human herpesviruses presumably antigenically similar to mhv-68, as herpes simplex virus 1 (hsv-1), human cytomegalovirus (hcmv), and epstein-barr virus (ebv), in general population usin ... | 2007 | 18197737 |
| successful treatment of neonatal herpes simplex-type 1 infection complicated by hemophagocytic lymphohistiocytosis and acute liver failure. | neonatal disseminated herpes simplex virus (hsv) infection with acute liver failure (alf) and neonatal hemophagocytic lymphohistiocytosis (hlh) are severe diseases. we recently experienced a male infant with hlh and alf induced by hsv type 1 (hsv-1). the infant, born at 39 weeks of gestation by normal delivery, developed a fever on day 4. on day 9, laboratory investigations showed progressive liver dysfunction and coagulopathy, and the serum ferritin was excessively elevated. furthermore, the bl ... | 2008 | 18212481 |
| knockin mice expressing a chimeric p53 protein reveal mechanistic differences in how p53 triggers apoptosis and senescence. | the contribution of transcriptional activation to the p53 effector functions critical for tumor suppression, apoptosis and cellular senescence, remains unclear because of p53's ability to regulate diverse cellular processes in a transactivation-independent manner. dissociating the importance of transactivation from other p53 functions, including regulating transcriptional repression, dna replication, homologous recombination, centrosome duplication, and mitochondrial function, has been difficult ... | 2008 | 18216268 |
| synthesis of 99mtc(co)3-deoxyuridine derivatives as potential hsv1-tk gene expression imaging agents. | in this study, we synthesized (99m)tc(co)(3)-2'-aminomethylpyridyl-2'-deoxyuridine ((99m)tc(co)(3)-ampdu) and (99m)tc(co)(3)-aminoethylpyridyl-2'-deoxyuridine ((99m)tc(co)(3)-aepdu) as potential agents for imaging the expression of the non-invasive herpes simplex virus type-1 thymidine kinase. ampdu and aepdu were synthesized from uridine in five chemical steps and then labeled with [(99m)tc(co)(3)(h(2)o)(3)](+) (370mbq/0.5 ml) at 100 degrees c for 10 min. under optimal conditions (0.5 and 1.0mg ... | 2008 | 18222693 |
| attenuated multimutated herpes simplex virus-1 effectively treats prostate carcinomas with neural invasion while preserving nerve function. | many cancers can cause disability and pain by invading nerves. in particular, prostate carcinoma has a high propensity for neural invasion (ni) at an early stage. attempted surgical treatment of tumors with ni often leads to erectile dysfunction and deteriorated quality of life. therefore, there is a need for novel modalities that will selectively target cancer cells while preserving neural function. herpes simplex viruses (hsvs) have a natural trophism for peripheral nerves. we hypothesized tha ... | 2008 | 18234972 |
| detection of herpes virus dna in post-operative thyroid tissue specimens of patients with autoimmune thyroid disease. | to demonstrate any differences in the detection of herpes simplex virus type 1 and 2, cytomegalovirus, human herpes virus type 6 and 7 dna from thyroid tissue blocks of patients with autoimmune thyroid disease and multi-nodular goiter and to propose few mechanisms, which could explain the possible role of herpesvirus infection in the development of thyroid autoimmune responses. | 2008 | 18240111 |
| an hsv-1 gd mutant virus as an entry-impaired live virus vaccine. | hsv-1 glycoprotein d (gd) interacts with hvem and nectin-1 cell receptors to initiate virus entry. we prepared an hsv-1 strain with mutations in the gd gene at amino acid residues 3 and 38 by changing alanine to cysteine and tyrosine to cysteine, respectively (a3c/y38c). these mutations were constructed with the intent of evaluating infection in vivo when virus enters by hvem but not nectin-1 receptors and were based on prior reports demonstrating that purified gda3c/y38c protein binds to hvem b ... | 2008 | 18243431 |
| human cytomegalovirus-specific cd4+ and cd8+ t-cell reconstitution in adult allogeneic hematopoietic stem cell transplant recipients and immune control of viral infection. | human cytomegalovirus infection is the most frequent viral complication in patients undergoing hematopoietic stem cell transplantation. we investigated the development of human cytomegalovirus-specific t cells in adult recipients of hematopoietic stem cell transplants. | 2008 | 18245650 |
| sequences in the ul11 tegument protein of herpes simplex virus that control association with detergent-resistant membranes. | the product of the ul11 gene of hsv-1 is a small, membrane-bound tegument protein with features that are conserved among all herpesviruses. for all viruses examined, mutants lacking this protein (or its homolog) have budding defects and accumulate capsids in the cytoplasm of the infected cell. ul11 binds to the cytoplasmic faces of host membranes via n-terminal myristate and nearby palmitate moieties. these fatty-acid modifications are typical of proteins that localize to detergent-resistant mem ... | 2008 | 18261757 |
| apoptosis regulators fas and bim cooperate in shutdown of chronic immune responses and prevention of autoimmunity. | apoptotic death of t lymphocytes is critical for shutdown of immune responses and hemopoietic cell homeostasis. both death receptor (fas) activation and mitochondrial apoptosis triggered by the bh3-only protein bim have been implicated in the killing of antigen-stimulated t cells. we examined mice lacking the gene encoding bim (bcl2l11) and with the inactivating lpr mutation in the gene encoding fas (fas), designated bcl2l11(-/-)fas(lpr/lpr) mice. shutdown of an acute t cell response to herpes s ... | 2008 | 18275830 |
| identification of two small rnas within the first 1.5-kb of the herpes simplex virus type 1-encoded latency-associated transcript. | the herpes simplex virus type 1 (hsv-1) latency-associated transcript (lat) is abundantly expressed in latently infected neurons. in the rabbit or mouse ocular models of infection, expression of the first 1.5 kb of lat coding sequences is sufficient for and necessary for wild-type levels of spontaneous reactivation from latency. the antiapoptosis functions of lat, which maps to the same 1.5 kb of lat, are important for the latency-reactivation cycle because replacement of lat with other antiapop ... | 2008 | 18300074 |
| prevalence of human papillomavirus and herpes simplex virus type 2 infection in korean commercial sex workers. | in order to investigate the prevalence of sexually transmitted viruses such as human papillomavirus (hpv) and herpes simplex virus (hsv) in korean commercial sex workers (csws), we selected 188 csws (age range 20-44 years, median age 24 years) who regularly visited one public health center in seoul, korea. hpv genotypes were analyzed by using a hpv dna chip, and an enzyme-linked immunosorbent assay (elisa) was used to detect type-specific igg against hsv2 antibody identifying seropositivity for ... | 2008 | 18309283 |
| the identification and characterization of fusogenic domains in herpes virus glycoprotein b molecules. | the molecular mechanism of entry of herpes viruses requires a multicomponent fusion system. virus entry and cell-cell fusion of herpes simplex virus (hsv) requires four glycoproteins: gd, gb and gh/gl. the role of gb remained elusive until recently, when the crystal structure of hsv-1 gb became available. glycoprotein b homologues represent the most highly conserved group of herpes virus glycoproteins; however, despite the high degree of sequence and structural conservation, differences in post- ... | 2008 | 18311743 |
| herpes simplex labialis in children with acute falciparum malaria. | 2008 | 18313630 | |
| antiviral activities of coffee extracts in vitro. | both hot water extracts of coffee grinds and instant coffee solutions inhibited the multiplication of herpes simplex virus type 1, a representative enveloped dna virus, when they were added to the culture medium of the virus-infected cells at a dose of one fifth the concentration suitable for drinking. the antiherpetic activity was independent of the suppliers (companies) of the coffee grinds and of the locations where the coffee beans were produced. further characterization revealed that there ... | 2008 | 18314244 |
| phenoxazine derivatives inactivate human cytomegalovirus, herpes simplex virus-1, and herpes simplex virus-2 in vitro. | we examined whether phenoxazine derivatives, 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3h-phenoxazine-3-one (phx-1), 3-amino-1,4alpha-dihydro-4alpha-8-dimethyl-2h-phenoxazine-2-one (phx-2), and 2-amino-phenoxazine-3-one (phx-3) may have antiviral activity against herpes family viruses: human cytomegalovirus (hcmv), herpes simplex virus type 1 (hsv-1), and herpes simplex virus type 2 (hsv-2). the antiviral activity was evaluated by the selectivity index (si), which is the ratio of 50% cytotoxic ... | 2008 | 18319567 |
| uniocular anterior chamber inoculation of a tumor necrosis factor alpha-expressing recombinant of herpes simplex virus type 1 results in more rapid destruction and increased viral replication in the retina of the uninoculated eye. | tumor necrosis factor alpha (tnf-alpha) has been shown to have a protective role in the eyes and brains of herpes simplex virus type 1 (hsv-1)-infected mice. to determine whether overexpression of tnf-alpha affected the course of virus infection following uniocular anterior chamber inoculation, a recombinant of hsv-1 that produces tnf-alpha constitutively (kostnf) was constructed. balb/c mice were injected with the tnf-alpha recombinant, a recombinant containing the pci plasmid, a recombinant re ... | 2008 | 18321975 |
| evaluation of real-time polymerase chain reaction assays for the detection of herpes simplex virus in swab specimens. | an in-house herpes simplex virus (hsv) real-time polymerase chain reaction (pcr) hydrolysis probe assay and three commercial real-time hsv assays were evaluated for the detection of hsv from genital, oral, ocular and dermal clinical samples. five hundred and sixty samples from patients displaying signs and symptoms of hsv infection were used to evaluate the in-house hsv assay. a representative 151 samples were processed using the artus hsv-1/2 tm pcr kit, smartcycler non-typing and smartcycler t ... | 2008 | 18322714 |
| alzheimer's disease-like changes in herpes simplex virus type 1 infected cells: the case for antiviral therapy. | 2008 | 18328007 | |
| the in vitro anti-herpes simplex virus type-1 and type-2 activity of long dan xie gan tan, a prescription of traditional chinese medicine. | long dan xie gan tan (ldxgt), a decoction of radix gentianae for purging the pathogenic inflammation of the liver, is a widely used prescription among many in traditional chinese medicine. the prescription is primarily used to treat the disorders induced by damp-heat in the liver and the gall bladder. | 2008 | 18332627 |
| genotypic detection of acyclovir-resistant hsv-1: characterization of 67 acv-sensitive and 14 acv-resistant viruses. | infections due to herpes simplex virus (hsv) resistant to acyclovir (acv) represent an important clinical concern in immunocompromised patients. in order to switch promptly to an appropriate treatment, rapid viral susceptibility assays are required. we developed herein a genotyping analysis focusing on thymidine kinase gene (tk) mutations in order to detect acyclovir-resistant hsv in clinical specimens. a total of 85 hsv-1 positive specimens collected from 69 patients were analyzed. tk gene coul ... | 2008 | 18336925 |
| live visualization of herpes simplex virus type 1 compartment dynamics. | we have constructed a recombinant herpes simplex virus type 1 (hsv-1) that simultaneously encodes selected structural proteins from all three virion compartments-capsid, tegument, and envelope-fused with autofluorescent proteins. this triple-fluorescent recombinant, rhsv-ryc, was replication competent, albeit with delayed kinetics, incorporated the fusion proteins into all three virion compartments, and was comparable to wild-type hsv-1 at the ultrastructural level. the vp26 capsid fusion protei ... | 2008 | 18337577 |
| [the n1-18 terminus of marek's disease virus vp22 is essential for protein transduction]. | we previously showed some differences in marek's disease virus (mdv) vp22 gene between virulent and avirulent strains, in the deletion from 201aa to 206aa, namely 201tksert206. in this study, vp22 genes were amplified from strains: cvi988/rispens and ga. and then the fragments were subcloned into pcdna3.1/zeo(+), respectively, which were co-expressed with an enhancer green fluorescent protein (egfp) after transfection into cos-1 cells. as with both human herpesvirus 1 and bovine herpesvirus 1 vp ... | 2008 | 18338583 |
| entry of herpesviruses into mammalian cells. | the mechanism that herpesviruses use to enter cells is one of the most complex viral entry mechanisms studied so far. this complexity seems to mount as new participants, both cellular receptors and viral glycoproteins, are identified. recent structural work on entry glycoproteins gd and gb from herpes simplex virus (hsv) 1 has illuminated the functional roles of these glycoproteins in the process of entry. in doing so, it provided information on the mechanism of two critical steps of hsv entry: ... | 2008 | 18351291 |
| [zao "ekolab" enzyme immunoassay systems for the diagnosis of herpes simplex]. | the laboratory diagnosis of herpes has recently ranked among the most topical problems of modern public health care since the role of the above infection, as other torch-group infections, is constantly increasing in the development of maternal and infant diseases. for the laboratory diagnosis of herpes, zao "ekolab" has proposed the enzyme immunoassay systems "eia-hsv-1-igg", "eia-hsv-2-igg", and "eia-hsv-1,2-igm" that allow for making type-specific diagnosis of herpes and for determining the ch ... | 2008 | 18354919 |
| pilralpha is a herpes simplex virus-1 entry coreceptor that associates with glycoprotein b. | glycoprotein b (gb) is one of the essential components for infection by herpes simplex virus-1 (hsv-1). although several cellular receptors that associate with glycoprotein d (gd), such as herpes virus entry mediator (hvem) and nectin-1, have been identified, specific molecules that mediate hsv-1 infection by associating with gb have not been elucidated. here, we found that paired immunoglobulin-like type 2 receptor (pilr) alpha associates with gb, and cells transduced with pilralpha become susc ... | 2008 | 18358807 |
| a-5021: a new acyclovir analogue inhibits murine herpetic keratitis. | to determine the efficacy of a-5021, a new analogue of acyclovir, on murine herpetic keratitis. | 2008 | 18362663 |
| escherichia coli nissle 1917 facilitates tumor detection by positron emission tomography and optical imaging. | bacteria-based tumor-targeted therapy is a modality of growing interest in anticancer strategies. imaging bacteria specifically targeting and replicating within tumors using radiotracer techniques and optical imaging can provide confirmation of successful colonization of malignant tissue. | 2008 | 18369089 |
| [pharmacological treatment of bell's palsy: favourable effects of prednisolone-based therapy now demonstrated]. | bell's palsy accounts for two-thirds ofall acute facial palsies. presumed reactivation of the herpes simplex virus and concurrent swelling of the facial nerve prompted the use of antivirals in combination with corticosteroids, although evidence supporting the effectiveness of this approach was weak. a recently published randomized placebo-controlled clinical trial assessed the effectiveness of adding valacyclovir to prednisolone; another larger primary-care-based study compared treatment with pr ... | 2008 | 18380381 |
| an intranasal heat shock protein based vaccination strategy confers protection against mucosal challenge with herpes simplex virus. | herpes simplex virus-1 (hsv-1) represents a significant obstacle for vaccine designers, despite decades of investigation. the virus primarily infects the host at vulnerable mucosal surfaces that progresses to lesion development, latency in nervous tissue, and possible reactivation. therefore, protection at the site of infection is crucial. mucosal adjuvants are critical for the development of an effective vaccine approach and heat-shock protein 70 (hsp70) represents an attractive candidate for t ... | 2008 | 18382144 |
| a fusogenic segment of glycoprotein h from herpes simplex virus enhances transfection efficiency of cationic liposomes. | low cellular uptake and endosomal escape are regarded as major limitations for nonviral gene delivery. in the present study, we use cationic liposomes incorporating fusogenic peptides from glycoprotein h of herpes simplex virus to improve cellular internalization and endosomal release of cationic liposome-dna complexes. | 2008 | 18383507 |
| activated inflammatory infiltrate in hsv-1-infected corneas without herpes stromal keratitis. | to investigate herpes stromal keratitis (hsk) immunopathology by studying hsv-1-infected corneas that fail to develop hsk. | 2008 | 18385067 |
| herpes simplex type 1 versus herpes simplex type 2 in anogenital herpes; a 10 year study from the waikato region of new zealand. | to determine the proportion of herpes simplex type 1 vs herpes simplex type 2, as a cause of anogenital herpes in the waikato region. we specifically looked for changes in the proportions over time, and for gender or age group associations. | 2008 | 18392061 |
| partial functional complementation of a pseudorabies virus ul25 deletion mutant by herpes simplex virus type 1 pul25 indicates overlapping functions of alphaherpesvirus pul25 proteins. | homologs of the ul25 gene product of herpes simplex virus 1 (hsv-1) are highly conserved among the herpesviridae. however, their exact function during viral replication is unknown. current evidence suggests that in the alphaherpesvirus pseudorabies virus (prv) the capsid-associated pul25 plays a role in primary envelopment of dna-containing mature capsids at the inner nuclear membrane. in the absence of pul25, capsids were found in close association with the inner nuclear membrane, but nuclear e ... | 2008 | 18400859 |
| use of multiplex pcr and real-time pcr to detect human herpes virus genome in ocular fluids of patients with uveitis. | to measure the genomic dna of human herpes viruses (hhv) in the ocular fluids and to analyse the clinical relevance of hhv in uveitis. | 2008 | 18408082 |