Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| a new family of insect muscarinic acetylcholine receptors. | most currently used insecticides are neurotoxic chemicals that target a limited number of sites and insect cholinergic neurotransmission is the major target. a potential target for insecticide development is the muscarinic acetylcholine receptor (machr), which is a metabotropic g-protein-coupled receptor. insects have a- and b-type machrs and the five mammalian machrs are close to the a-type. we isolated a cdna (cg12796) from the fruit fly, drosophila melanogaster. after heterologous expression ... | 2016 | 27003873 |
| genetic mutation analysis at early stages of cell line development using next generation sequencing. | a central goal for most biopharmaceutical companies is to reduce the development timeline to reach clinical proof of concept. this objective requires the development of tools that ensure the quality of biotherapeutic material destined for the clinic. recent advances in high throughput protein analytics provide confidence in our ability to assess productivity and product quality attributes at early stages of cell line development. however, one quality attribute has, until recently, been absent fr ... | 2016 | 27004436 |
| glycan-deficient prp stimulates vegfr2 signaling via glycosaminoglycan. | whether the two n-linked glycans are important in prion, prp, biology is unresolved. in chinese hamster ovary (cho) cells, the two glycans are clearly not important in the cell surface expression of transfected human prp. compared to fully-glycosylated prp, glycan-deficient prp preferentially partitions to lipid raft. in cho cells glycan-deficient prp also interacts with glycosaminoglycan (gag) and vascular endothelial growth factor receptor 2 (vegfr2), resulting in vegfr2 activation and enhance ... | 2016 | 27006333 |
| biophysical studies on calcium and carbohydrate binding to carbohydrate recognition domain of gal/galnac lectin from entamoeba histolytica: insights into host cell adhesion. | entamoeba histolytica, an enteric parasite expresses a gal/galnac-specific lectin that contributes to its virulence by establishing adhesion to host cell. in this study, carbohydrate recognition domain of hgl (ehcrd) was purified and biophysical studies were conducted to understand the thermodynamic basis of its binding to carbohydrate and ca(++) here, we show that carbohydrate recognition domain (crd) of the lectin binds to calcium through dpn motif. to decipher the role of calcium in carbohydr ... | 2016 | 27008865 |
| putting the spotlight back on plant suspension cultures. | plant cell suspension cultures have several advantages that make them suitable for the production of recombinant proteins. they can be cultivated under aseptic conditions using classical fermentation technology, they are easy to scale-up for manufacturing, and the regulatory requirements are similar to those established for well-characterized production systems based on microbial and mammalian cells. it is therefore no surprise that taliglucerase alfa (elelyso®)-the first licensed recombinant ph ... | 2016 | 27014320 |
| oxytocin neuron activation prevents hypertension that occurs with chronic intermittent hypoxia/hypercapnia in rats. | hypertension is a common outcome associated with obstructive sleep apnea (osa), a prevalent yet poorly treated cardiovascular disease. recent studies showed oxytocin (oxt), released from hypothalamic paraventricular nucleus (pvn) neurons, activates cardiac vagal neurons in the dorsal motor nucleus of the vagus (dmnx) and may blunt cardiovascular responses to stress. this study tests whether the release of oxt from pvn fibers in the dmnx is diminished with chronic intermittent hypoxia-hypercapnia ... | 2016 | 27016581 |
| characterization of melanocortin receptors from stingray dasyatis akajei, a cartilaginous fish. | melanocortin (mc) systems are composed of mc peptides such as adrenocorticotropic hormone (acth), several molecular forms of melanocyte-stimulating hormones (mshs) and mc receptors (mcrs). here we demonstrated that the cartilaginous fish, dasyatis akajei (stingray) expresses five subtypes of mcr genes-mc1r to mc5r-as in the case of teleost and tetrapod species. this is the first evidence showing the presence of the full repertoire of melanocortin receptors in a single of cartilaginous fish. expr ... | 2016 | 27021018 |
| antioxidant and dna repair stimulating effect of extracts from transformed and normal roots of rhaponticum carthamoides against induced oxidative stress and dna damage in cho cells. | rhaponticum carthamoides has a long tradition of use in siberian folk medicine. the roots and rhizomes of this species are used in various dietary supplements or nutraceutical preparations to increase energy level or eliminate physical weakness. this is the first report to reveal the protective and dna repair stimulating abilities of r. carthamoides root extracts in chinese hamster ovary (cho) cells exposed to an oxidative agent. both transformed root extract (tr extract) and extract of soil-gro ... | 2016 | 27034736 |
| helium ions for radiotherapy? physical and biological verifications of a novel treatment modality. | modern facilities for actively scanned ion beam radiotherapy allow in principle the use of helium beams, which could present specific advantages, especially for pediatric tumors. in order to assess the potential use of these beams for radiotherapy, i.e., to create realistic treatment plans, the authors set up a dedicated (4)he beam model, providing base data for their treatment planning system trip98, and they have reported that in this work together with its physical and biological validations. | 2016 | 27036594 |
| cell growth on 3d microstructured surfaces. | chinese hamster ovary (cho) cell cultures were grown on surfaces lithographed with periodic 3d hexagonal microcavity array morphology. the range of microcavity size (inscribed circle diameter) was from 12 μm to 560 μm. cho cells were grown also on flat surfaces. the characterization was performed with respect to cell growth density (number of nuclei per unit area) by fluorescence optical microscopy and evaluated by correlation function analysis. we found that optimum microcavity radius was 80 μm ... | 2016 | 27040266 |
| detection and quantification of adp-ribosylated rhoa/b by monoclonal antibody. | clostridium botulinum exoenzyme c3 is the prototype of c3-like adp-ribosyltransferases that modify the gtpases rhoa, b, and c. c3 catalyzes the transfer of an adp-ribose moiety from the co-substrate nicotinamide adenine dinucleotide (nad) to asparagine-41 of rho-gtpases. although c3 does not possess cell-binding/-translocation domains, c3 is able to efficiently enter intact cells, including neuronal and macrophage-like cells. conventionally, the detection of c3 uptake into cells is carried out v ... | 2016 | 27043630 |
| measurement of rapid amiloride-dependent ph changes at the cell surface using a proton-sensitive field-effect transistor. | we present a novel method for the rapid measurement of ph fluxes at close proximity to the surface of the plasma membrane in mammalian cells using an ion-sensitive field-effect transistor (isfet). in conjuction with an efficient continuous superfusion system, the isfet sensor was capable of recording rapid changes in ph at the cells' surface induced by intervals of ammonia loading and unloading, even when using highly buffered solutions. furthermore, the system was able to isolate physiologicall ... | 2016 | 27043644 |
| biphasic cultivation strategy to avoid epo-fc aggregation and optimize protein expression. | in biphasic cultivations, the culture conditions are initially kept at an optimum for rapid cell growth and biomass accumulation. in the second phase, the culture is shifted to conditions ensuring maximum specific protein production and the protein quality required. the influence of specific culture parameters is cell line dependent and their impact on product quality needs to be investigated. in this study, a biphasic cultivation strategy for a chinese hamster ovary (cho) cell line expressing a ... | 2016 | 27050504 |
| characterization of alanine to valine sequence variants in the fc region of nivolumab biosimilar produced in chinese hamster ovary cells. | nivolumab is a therapeutic fully human igg4 antibody to programmed death 1 (pd-1). in this study, a nivolumab biosimilar, which was produced in our laboratory, was analyzed and characterized. sequence variants that contain undesired amino acid sequences may cause concern during biosimilar bioprocess development. we found that low levels of sequence variants were detected in the heavy chain of the nivolumab biosimilar by ultra performance liquid chromatography (uplc) and tandem mass spectrometry. ... | 2016 | 27050807 |
| physiological modulators of kv3.1 channels adjust firing patterns of auditory brain stem neurons. | many rapidly firing neurons, including those in the medial nucleus of the trapezoid body (mntb) in the auditory brain stem, express "high threshold" voltage-gated kv3.1 potassium channels that activate only at positive potentials and are required for stimuli to generate rapid trains of actions potentials. we now describe the actions of two imidazolidinedione derivatives, aut1 and aut2, which modulate kv3.1 channels. using chinese hamster ovary cells stably expressing rat kv3.1 channels, we found ... | 2016 | 27052580 |
| polymorphisms in dna repair gene and susceptibility to glioma: a systematic review and meta-analysis based on 33 studies with 15 snps in 9 genes. | at present, many publications have evaluated the correlation between the dna repair gene polymorphisms and glioma susceptibility. however, the results remain inconclusive. the aim of this research is to exhaustively assess the association of genetic polymorphisms in dna repair genes with glioma risk in human. meta-analysis method was conducted, and 33 studies with 15 snps in 9 genes were included (12553 glioma cases and 17178 controls). correlation strength was evaluated by odds ratio with a 95 ... | 2017 | 27055523 |
| polysorbates 20 and 80 degradation by group xv lysosomal phospholipase a2 isomer x1 in monoclonal antibody formulations. | decreases in polysorbate (ps80) content were observed while evaluating the long-term storage stability of chinese hamster ovary-derived, purified monoclonal antibodies. it was determined that polysorbate had been enzymatically degraded; therefore, studies were performed to identify and characterize the protein(s) responsible. polysorbate degrading activity was enriched from chinese hamster ovary media leading to the identification of group xv lysosomal phospholipase a2 isomer x1 (lpla2) by shotg ... | 2016 | 27056628 |
| poly-pathway model, a novel approach to simulate multiple metabolic states by reaction network-based model - application to amino acid depletion in cho cell culture. | mammalian cell lines are characterized by a complex and flexible metabolism. a single model that could describe the variations in metabolic behavior triggered by variations in the culture conditions would be a precious tool in bioprocess development. in this paper, we introduce an approach to generate a poly-pathway model and use it to simulate diverse metabolic states triggered in response to removal, reduction or doubling of amino acids in the culture medium of an antibody-producing cho cell l ... | 2016 | 27060554 |
| characterization of recombinant human diamine oxidase (rhdao) produced in chinese hamster ovary (cho) cells. | human diamine oxidase (hdao) efficiently degrades polyamines and histamine. reduced enzyme activities might cause complications during pregnancy and be involved in histamine intolerance. so far hdao has been characterized after isolation from either native sources or the heterologous production in insect cells. accessibility to human enzyme is limited and insect cells produce non-human glycosylation patterns that may alter its biochemical properties. we present the heterologous expression of hda ... | 2016 | 27063138 |
| isotetrandrine reduces astrocyte cytotoxicity in neuromyelitis optica by blocking the binding of nmo-igg to aquaporin 4. | neuromyelitis optica (nmo) is a severe neurological demyelinating autoimmune disease that affects the optic nerves and spinal cord with no cure and no fda-approved therapy. research over the last decade revealed that the binding of nmo-igg to the water channel protein astrocyte aquaporin 4 (aqp4) might be the primary cause of nmo pathogenesis. the purpose of this study was to identify potential blockers of nmo-igg and aqp4 binding. | 2016 | 27064690 |
| impact of hydrolysates on monoclonal antibody productivity, purification and quality in chinese hamster ovary cells. | plant and yeast derived hydrolysates are economical and efficient alternative medium supplements to improve mammalian cell culture performance. we supplemented two commercial chinese hamster ovary (cho) culture media with hydrolysates from four different sources, yeast, soybean, ex-cell cd (a chemically defined hydrolysate replacement) and wheat to improve the productivity of two cell lines expressing different monoclonal antibodies (mabs). yeast, soybean and ex-cell cd improved the final mab ti ... | 2016 | 27067279 |
| quantitative analysis of the supernatant from host and transfected cho cells using itraq 8-plex technique. | we employed uplc-ms/ms with itraq 8-plex labeling to quantitatively analyze the supernatant produced by two chinese hamster ovary (cho) cell lines (cho k1sv and cho cat-s). in each case, the supernatant from the host and three transfected clones were analyzed at days 5, 7, and 10 of culture. a total of eight itraq 8-plex experiments were performed. for each cell line, the overlap of supernatant protein identifications between transfected clones is over 60%. over 70% of the supernatant proteins i ... | 2016 | 27070921 |
| comprehensive genome and epigenome characterization of cho cells in response to evolutionary pressures and over time. | the most striking characteristic of cho cells is their adaptability, which enables efficient production of proteins as well as growth under a variety of culture conditions, but also results in genomic and phenotypic instability. to investigate the relative contribution of genomic and epigenetic modifications towards phenotype evolution, comprehensive genome and epigenome data are presented for six related cho cell lines, both in response to perturbations (different culture conditions and media a ... | 2016 | 27072894 |
| high levels of histone h3 acetylation at the cmv promoter are predictive of stable expression in chinese hamster ovary cells. | chinese hamster ovary cells (cho) are widely used in the production of glycosylated therapeutic proteins such as antibodies. during expansion and maintenance, cho cell lines are prone to production instability, which may be caused by promoter silencing, loss of transgene copies, or post-transcriptional effects. silencing of recombinant genes may be accompanied by dna methylation and histone modification. previously, we demonstrated that cytosine methylation of the human cytomegalovirus major imm ... | 2016 | 27073121 |
| one-step conjugation method for site-specific antibody-drug conjugates through reactive cysteine-engineered antibodies. | engineered cysteine residues are particularly convenient for site-specific conjugation of antibody-drug conjugates (adc), because no cell engineering and additives are required. usually, unpaired cysteine residues form mixed disulfides during fermentation in chinese hamster ovarian (cho) cells; therefore, additional reduction and oxidization steps are required prior to conjugation. in this study, we prepared light chain (lc)-q124c variants in igg and examined the conjugation efficiency. intrigui ... | 2016 | 27074832 |
| the long intron 1 of growth hormone gene from reeves' turtle (chinemys reevesii) correlates with negatively regulated gh expression in four cell lines. | turtles grow slowly and have a long lifespan. ultrastructural studies of the pituitary gland in reeves' turtle (chinemys reevesii) have revealed that the species possesses a higher nucleoplasmic ratio and fewer secretory granules in growth hormone (gh) cells than other animal species in summer and winter. c. reevesii gh gene was cloned and species-specific similarities and differences were investigated. the full gh gene sequence in c. reevesii contains 8517 base pairs (bp), comprising five exons ... | 2016 | 27077853 |
| identification of drug-like inhibitors of insulin-regulated aminopeptidase through small-molecule screening. | intracerebroventricular injection of angiotensin iv, a ligand of insulin-regulated aminopeptidase (irap), has been shown to improve cognitive functions in several animal models. consequently, irap is considered a potential target for treatment of cognitive disorders. to identify nonpeptidic irap inhibitors, we adapted an established enzymatic assay based on membrane preparations from chinese hamster ovary cells and a synthetic peptide-like substrate for high-throughput screening purposes. the 38 ... | 2016 | 27078680 |
| dimerization of melanocortin receptor 1 (mc1r) and mc5r creates a ligand-dependent signal modulation: potential participation in physiological color change in the flounder. | vertebrates produce α-melanocyte-stimulating hormone (α-msh), which contains an n-terminal acetyl group, and desacetyl-α-msh, which does not contain an n-terminal acetyl group. in teleosts and amphibians, α-msh-related peptides stimulate pigment dispersion via melanocortin receptors 1-5 (mc1r-mc5r), which are members of the g-protein-coupled receptor (gpcr) family. we previously reported an interesting phenomenon associated with physiological color changes in the skin of a flatfish, barfin floun ... | 2016 | 27080548 |
| mutation detection in an antibody-producing chinese hamster ovary cell line by targeted rna sequencing. | chinese hamster ovary (cho) cells have been used widely in the pharmaceutical industry for production of biological therapeutics including monoclonal antibodies (mab). the integrity of the gene of interest and the accuracy of the relay of genetic information impact product quality and patient safety. here we employed next-generation sequencing, particularly rna-seq, and developed a method to systematically analyze the mutation rate of the mrna of cho cell lines producing a mab. the effect of an ... | 2016 | 27088091 |
| cb1 cannabinoid receptor-mediated increases in cyclic amp accumulation are correlated with reduced gi/o function. | cb1 cannabinoid receptors (cb1rs) stimulate gi/o-dependent signaling pathways. cb1r-mediated camp increases were proposed to result from gs activation, but cb1r-stimulated gtpγs binding to gs has not heretofore been investigated. | 2016 | 27089415 |
| identifying the differences in mechanisms of mycophenolic acid controlling fucose content of glycoproteins expressed in different cho cell lines. | in the biopharmaceutical industry, glycosylation is a critical quality attribute that can modulate the efficacy of a therapeutic glycoprotein. obtaining a consistent glycoform profile is desired because molecular function can be defined by its carbohydrate structures. specifically, the fucose content of oligosaccharides in glycoproteins is one of the most important attributes that can significantly affect antibody-dependent cellular cytotoxicity (adcc) activity. it is therefore important to unde ... | 2016 | 27093551 |
| influenza virus hemagglutinin glycoproteins with different n-glycan patterns activate dendritic cells in vitro. | influenza virus hemagglutinin (ha) n-glycans play important regulatory roles in the control of virus virulence, antigenicity, receptor-binding specificity, and viral escape from the immune response. considered essential for controlling innate and adaptive immune responses against influenza virus infections, dendritic cells (dcs) trigger proinflammatory and adaptive immune responses in hosts. in this study, we engineered chinese hamster ovary (cho) cell lines expressing recombinant ha from pandem ... | 2016 | 27099319 |
| novel citronellyl-based photoprobes designed to identify er proteins interacting with dolichyl phosphate in yeast and mammalian cells. | dolichyl phosphate-linked mono- and oligosaccharides (dlo) are essential intermediates in protein n-glycosylation, c- and o-mannosylation and gpi anchor biosynthesis. while many membrane proteins in the endoplasmic reticulum (er) involved in the assembly of dlos are known, essential proteins believed to be required for the transbilayer movement (flip-flopping) and proteins potentially involved in the regulation of dlo synthesis remain to be identified. | 2017 | 27099830 |
| study on physiological roles of stimulation of prostaglandin e2 receptor subtype ep2 in urethral function in rats. | we investigated the relaxant effect of stimulation of prostaglandin e2 (pge2 ) receptor subtype ep2 as well as the involvement of a cyclic amp (camp)-dependent pathway related to stimulation of ep2 receptors in urethral function in rats by evaluating effects of pge2 and selective ep2 receptor agonist cp-533,536. | 2016 | 27111625 |
| expression and bioactivity of recombinant human serum albumin and dtmp fusion proteins in cho cells. | the 14-amino acid (iegptlrqwlaara) thrombopoietin mimetic peptide (tmp) shares no sequence homology with native thrombopoietin (tpo). when dimerized, it displays a high-binding affinity for the tpo receptor and has equipotent bioactivity with recombinant human tpo (rhtpo) in stimulating proliferation and maturation of megakaryocytes in vitro. however, tmp is limited for clinical usage because of its short half-life in vivo. in this study, fusion proteins that composed of tandem dimer of tmp (dtm ... | 2016 | 27115755 |
| enhanced recombinant factor vii expression in chinese hamster ovary cells by optimizing signal peptides and fed-batch medium. | signal peptides play an important role in directing and efficiently transporting secretory proteins to their proper locations in the endoplasmic reticulum of mammalian cells. the aim of this study was to enhance the expression of recombinant coagulation factor vii (rfvii) in cho cells by optimizing the signal peptides and type of fed-batch culture medium used. five sub-clones (o2, i3, h3, g2 and m3) with different signal peptide were selected by western blot (wb) analysis and used for suspension ... | 2016 | 27116572 |
| neuro-immune interactions in chemical-induced airway hyperreactivity. | asthma may be induced by chemical sensitisers, via mechanisms that are still poorly understood. this type of asthma is characterised by airway hyperreactivity (ahr) and little airway inflammation. since potent chemical sensitisers, such as toluene-2,4-diisocyanate (tdi), are also sensory irritants, it is suggested that chemical-induced asthma relies on neuro-immune mechanisms.we investigated the involvement of transient receptor potential channels (trp) a1 and v1, major chemosensors in the airwa ... | 2016 | 27126687 |
| [stable expression of human anti-il-33 scfv-igg1fc fusion protein in cho k1 cells]. | to construct two different eukaryotic expression vectors of human anti-interleukin 33 (il-33) single-chain antibody fragment (scfv-fc) to transfect chinese hamster ovary (cho) k1 cells and select the stably and high-level expressed cell lines to improve the expression level of the fusion protein. | 2016 | 27126936 |
| a phase 1, randomized study to assess the pharmacokinetic comparability of siltuximab derived from two different cell lines in healthy subjects. | siltuximab, a monoclonal antibody (mab) against interleukin (il-6), is under development by janssen research & development, llc. during early clinical development, siltuximab was produced in a murine sp2/0 myeloma cell line. the production cell line was switched to stably transfected chinese hamster ovary (cho) cell line for subsequent clinical development. a two-part, parallel-group, phase 1 study was designed to evaluate the safety and pharmacokinetics (pk) of a single iv administration of sp2 ... | 2014 | 27128840 |
| asymmetrical flow field-flow fractionation coupled with multi-angle laser light scattering for stability comparison of virus-like particles in different solution environments. | the stabilities of two commercially available virus like particles, cho-hbsag expressed by chinese hamster ovary (cho) cells and hans-hbsag expressed by hansenula polymorpha (hans), were compared using af4-malls under different treatment processes. the initial molecular weight and hydrodynamic diameter of cho-hbsag measured with af4-malls were 4727kda and 29.4nm, while those of hans-hbsag were 3039kda and 22.8nm respectively. in salt solution of 2m ammonium sulfate, the molecular weight and size ... | 2016 | 27129428 |
| combined sodium ion sensitivity in agonist binding and internalization of vasopressin v1b receptors. | reducing na(+) in the extracellular environment may lead to two beneficial effects for increasing agonist binding to cell surface g-protein coupled receptors (gpcrs): reduction of na(+)-mediated binding block and reduce of receptor internalization. however, such combined effects have not been explored. we used chinese hamster ovary cells expressing vasopressin v1b receptors as a model to explore na(+) sensitivity in agonist binding and receptor internalization. under basal conditions, a large fr ... | 2016 | 27138239 |
| classification of normal and apoptotic cells from fluorescence microscopy images using generalized polynomial chaos and level set function. | accurate automated quantitative analysis of living cells based on fluorescence microscopy images can be very useful for fast evaluation of experimental outcomes and cell culture protocols. in this work, an algorithm is developed for fast differentiation of normal and apoptotic viable chinese hamster ovary (cho) cells. for effective segmentation of cell images, a stochastic segmentation algorithm is developed by combining a generalized polynomial chaos expansion with a level set function-based se ... | 2016 | 27142234 |
| highly luminescent heterostructured copper-doped zinc sulfide nanocrystals for application in cancer cell labeling. | the structural characteristics of the seed-mediated synthesis of heterostructured cus-zns nanocrystals (ncs) and cu-doped zns (zns:cu) ncs synthesized by two different protocols are compared and analyzed. at high cu dopant concentrations, segregated subclusters of zns and cus are observed. the photoluminescence quantum yield of zns:cu ncs is about 50-80 %; a value much higher than that of zns ncs (6 %). finally, these ncs are coated with a thin silica shell by using (3-mercaptopropyl)triethoxysi ... | 2016 | 27146419 |
| apolipoprotein e lipoprotein particles inhibit amyloid-β uptake through cell surface heparan sulphate proteoglycan. | the accumulation, aggregation and deposition of amyloid-β (aβ) peptides in the brain are central to the pathogenesis of alzheimer's disease (ad). alzheimer's disease risk increases significantly in individuals carrying one or two copies of apoe ε4 allele compared to individuals with an ε3/ε3 genotype. growing evidence has demonstrated that apolipoprotein e (apoe) strongly influences ad pathogenesis by controlling aβ aggregation and metabolism. heparan sulphate proteoglycans (hspgs) are abundant ... | 2016 | 27151330 |
| accelerated homology-directed targeted integration of transgenes in chinese hamster ovary cells via crispr/cas9 and fluorescent enrichment. | targeted gene integration into site-specific loci can be achieved in chinese hamster ovary (cho) cells via crispr/cas9 genome editing technology and the homology-directed repair (hdr) pathway. the low efficiency of hdr often requires antibiotic selection, which limits targeted integration of multiple genes at multiple sites. to improve hdr-mediated targeted integration, while avoiding the use of selection markers, chemical treatment for increased hdr, and fluorescent enrichment of genome-edited ... | 2016 | 27159230 |
| serum albumin attenuates the open-channel blocking effects of propofol on the human kv1.5 channel. | the intravenous anesthetic propofol modulates various ion channel functions. it is generally accepted that approximately 98% of propofol binds to blood constituents and that the free (unbound) drug preferentially affects target proteins including ion channels. however, modulatory effects of propofol on ion channels have not been previously explored in the presence of serum albumin. this study was designed to investigate the effects of serum albumin on the blocking action of propofol on the human ... | 2016 | 27164421 |
| organelle-targeted h2s probes enable visualization of the subcellular distribution of h2s donors. | hydrogen sulfide (h2s) is an essential biological signaling molecule in diverse biological regulatory pathways. to provide new chemical tools for h2s imaging, we report here a fluorescent h2s detection platform (hsn2-bg) that is compatible with subcellular localization snap-tag fusion protein methodologies and use appropriate fusion protein constructs to demonstrate mitochondrial and lysosomal localization. we also demonstrate the efficacy of this detection platform to image endogenous h2s in ch ... | 2016 | 27171507 |
| exposure to 50hz-sinusoidal electromagnetic field induces dna damage-independent autophagy. | as electromagnetic field (emf) is commonly encountered within our daily lives, the biological effects of emf are of great concern. autophagy is a key process for maintaining cellular homeostasis, and it can also reveal cellular responses to environmental stimuli. in this study, we aim to investigate the biological effects of a 50hz-sinusoidal electromagnetic field on autophagy and we identified its mechanism of action in chinese hamster lung (chl) cells. chl cells were exposed to a 50hz sinusoid ... | 2016 | 27177844 |
| disruption of the gene c12orf35 leads to increased productivities in recombinant cho cell lines. | recently, we reported that the loss of a telomeric region of chromosome 8 in chinese hamster ovary (cho) cells correlates with higher recombinant productivities. new cell lines lacking this region, called cho-c8del, showed several advantages during cell line generation and for the production of recombinant proteins (ritter et al., 2016, biotechnol bioeng). here, we performed knock-down and knock-out experiments of genes located within this telomeric region of chromosome 8 to identify the genes c ... | 2016 | 27183150 |
| in vitro evaluation of 2-hydroxyalkylated β-cyclodextrins as potential therapeutic agents for niemann-pick type c disease. | this study was conducted to evaluate the attenuating potential of 2-hydroxypropyl-β-cyclodextrin (hpbcd) against niemann-pick type c (npc) disease, as well as the physical and chemical properties, particularly the cholesterol-solubilizing ability, in an npc disease model in vitro. as parameters of npc abnormalities, intracellular free and esterified cholesterol levels and lysosome volume were measured in npc1 null chinese hamster ovary cells. hpbcd showed dose-dependent effects against dysfuncti ... | 2016 | 27184436 |
| comprehensive characterization of reference standard lots of hiv-1 subtype c gp120 proteins for clinical trials in southern african regions. | two hiv-1 subtype c gp120 protein candidates were the selected antigens for several experimental vaccine regimens now under evaluation in hvtn 100 phase i/ii clinical trial aiming to support the start of the hvtn 702 phase iib/iii trial in southern africa, which is designed to confirm and extend the partial protection seen against hiv-1 infection in the rv144 thai trial. here, we report the comprehensive physicochemical characterization of the gp120 reference materials that are representative of ... | 2016 | 27187483 |
| mutagenic properties of linuron and chlorbromuron evaluated by means of cytogenetic biomarkers in mammalian cell lines. | agricultural practices are usually supported by several chemical substances, such as herbicides. linuron and chlorbromuron are phenylurea herbicides largely used to protect crops from weeds, blocking photosynthesis by inhibition of the photosystem ii complex. the former, also commercially known as lorox or afalon, is selectively used to protect bean and french bean plants, fennels, and celeriacs; the second, commercially known as maloran, is selectively used for carrots, peas, potatoes, soy spro ... | 2016 | 27206752 |
| defective association of the platelet glycoprotein ib-ix complex with the glycosphingolipid-enriched membrane domain inhibits murine thrombus and atheroma formation. | localization of the platelet glycoprotein ib-ix complex to the membrane lipid domain is essential for platelet adhesion to von willebrand factor and subsequent platelet activation in vitro. yet, the in vivo importance of this localization has never been addressed. we recently found that the disulfide linkage between ibα and ibβ is critical for the association of ibα with the glycosphingolipid-enriched membrane domain; in this study, we established a transgenic mouse model expressing this mutant ... | 2016 | 27206768 |
| discovery and characterization of antibody variants using mass spectrometry-based comparative analysis for biosimilar candidates of monoclonal antibody drugs. | liquid chromatography mass spectrometry (lc-ms) is the most commonly used technique for the characterization of antibody variants. mab-x and mab-y are two approved igg1 subtype monoclonal antibody drugs recombinantly produced in chinese hamster ovary (cho) cells. we report here that two unexpected and rare antibody variants have been discovered during cell culture process development of biosimilars for these two approved drugs through intact mass analysis. we then used comprehensive mass spectro ... | 2016 | 27214604 |
| real-time monitoring of specific oxygen uptake rates of embryonic stem cells in a microfluidic cell culture device. | oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. quantification of cellular oxygen kinetics, i.e. the determination of specific oxygen uptake rates (sours), is routinely used to understand metabolic shifts. however current methods to determine sour in adherent cell cultures rely on cell sampling, which impacts on cellular phenotype. we present real-time monitoring of cell growth from phase contrast microscopy images, and of respi ... | 2016 | 27214658 |
| quantitative definition and monitoring of the host cell protein proteome using itraq - a study of an industrial mab producing cho-s cell line. | there are few studies defining cho host cell proteins (hcps) and the flux of these throughout a downstream purification process. here we have applied quantitative itraq proteomics to follow the hcp profile of an antibody (mab) producing cho-s cell line throughout a standard downstream purification procedure consisting of a protein a, cation and anion exchange process. we used both 6 sample itraq experiment to analyze technical replicates of three samples, which were culture harvest (hccf), prote ... | 2016 | 27214759 |
| hyperosmotic stimulus study discloses benefits in atp supply and reveals mirna/mrna targets to improve recombinant protein production of cho cells. | biopharmaceuticals are predominantly produced by chinese hamster ovary (cho) cells cultivated in fed-batch mode. hyperosmotic culture conditions (≥ 350 mosmol kg(∑1) ) resulting from feeding of nutrients may enhance specific product formation rates (qp ). as an improved atp supply was anticipated to enhance qp this study focused on the identification of suitable mirna/mrna targets to increase atp levels. therefor next generation sequencing and a compartment specific metabolomics approach were ap ... | 2016 | 27214792 |
| increased in vivo effector function of human igg4 isotype antibodies through afucosylation. | for some antibodies intended for use as human therapeutics, reduced effector function is desired to avoid toxicities that might be associated with depletion of target cells. since effector function(s), including antibody-dependent cell-mediated cytotoxicity (adcc), require the fc portion to be glycosylated, reduced adcc activity antibodies can be obtained through aglycosylation of the human igg1 isotype. an alternative is to switch to an igg4 isotype in which the glycosylated antibody is known t ... | 2017 | 27216702 |
| moderate and strong static magnetic fields directly affect egfr kinase domain orientation to inhibit cancer cell proliferation. | static magnetic fields (smfs) can affect cell proliferation in a cell-type and intensity-dependent way but the mechanism remains unclear. at the same time, although the diamagnetic anisotropy of proteins has been proposed decades ago, the behavior of isolated proteins in magnetic fields has not been directly observed. here we show that smfs can affect isolated proteins at the single molecular level in an intensity-dependent manner. we found that epidermal growth factor receptor (egfr), a protein ... | 2016 | 27223425 |
| expression and characterization of a potent long-acting glp-1 receptor agonist, glp-1-igg2σ-fc. | human glp-1 (glucagon-like peptide-1) can produce a remarkable improvement in glycemic control in patients with type 2 diabetes. however, its clinical benefits are limited by its short half-life, which is less than 2 min because of its small size and rapid enzymatic inactivation by dipeptidyl peptidase iv. we engineered glp-1-igg2σ-fc, a 68-kda fusion protein linking a variant human glp-1 (a8g/g26e/r36g) to a human igg2σ constant heavy-chain. a stably transfected chinese hamster ovary cell line ... | 2016 | 27232339 |
| novel glyceryl glucoside is a low toxic alternative for cryopreservation agent. | glyceryl glucoside (gg, α-d-glucosyglycerol) is a natural glycerol derivative found in alcoholic drinks. recently gg has been used as an alternative for glycerol in cosmetic products. however, the safety of using gg is still unclear. currently, dimethyl sulfoxide (dmso) and glycerol are wildly used in cryopreservation. despite gg being a derivative of glycerol, the ability of gg in cryopreservation is still unknown. by using a system of chinese hamster ovary cells (cho), a549 cells and ag1522 ce ... | 2016 | 27235553 |
| isolation and characterization of the corticotropin-releasing factor-related diuretic hormone receptor in rhodnius prolixus. | rhodnius prolixus, the vector of human chagas disease, is a hemipteran insect that undergoes rapid post-feeding diuresis following ingestion of a blood meal that can be up to 10 times its initial body weight. corticotropin-releasing factor-related diuretic hormone (rhopr-crf/dh) and serotonin are neurohormones that are synergistic in increasing rates of fluid secretion by malpighian tubules during this rapid post-feeding diuresis. a rhopr-crf/dh receptor transcript has now been isolated and char ... | 2016 | 27237375 |
| g-protein-dependency of orexin/hypocretin receptor signalling in recombinant chinese hamster ovary cells. | multiple signalling pathways for orexin receptors have been discovered, and most thoroughly mapped in chinese hamster ovary k1 (cho-k1) cells. it is also known that orexin receptors can couple to the g-protein families gi, gs and gq. however, the connection between the g-proteins and the downstream signals is only vaguely established, and we now set out to resolve this for human orexin receptors expressed in cho-k1 cells. adenylyl cyclase (ac), phospholipase a2, c and d, and diacylglycerol lipas ... | 2016 | 27237973 |
| contribution of antibody hydrodynamic size to vitreal clearance revealed through rabbit studies using a species-matched fab. | we have developed a tool fab fragment of a rabbit monoclonal antibody that is useful for early evaluation in rabbit models of technologies for long acting delivery (lad) of proteins to the eye. using this fab we show that vitreal clearance can be slowed through increased hydrodynamic size. fab (g10rabfab) and fab' (g10rabfab') fragments of a rabbit monoclonal antibody (g10rabigg) were expressed in chinese hamster ovary (cho) cells and purified using antigen-based affinity chromatography. g10rabf ... | 2016 | 27244474 |
| the displacement binding assay using human cannabinoid cb2 receptor-transfected cells. | displacement binding assays are nonfunctional assays mostly used with the aim of determining whether a certain compound (plant-derived or synthetic) is able to bind to a specific receptor with high affinity. here, we describe the displacement binding assay that is carried out with a radioligand and cho (chinese hamster ovarian) cells stably transfected with the human cannabinoid cb2 receptor. | 2016 | 27245891 |
| the cyclic amp assay using human cannabinoid cb2 receptor-transfected cells. | the cyclic amp assay is a functional assay that is commonly used to determine the pharmacological behavior (agonists, antagonists, inverse agonists) of g-protein-coupled receptor (gpcr) ligands. here, we describe the cyclic amp assay that is carried out with commercially available non-radioligand ready-to-use kits and chinese hamster ovarian (cho) cells stably transfected with the human cannabinoid cb2 receptor. | 2016 | 27245894 |
| application of unfolded protein response for production of biologics. | improvements in the productivity of established mammalian cell lines used for biopharmaceutical production are desirable to increase product yields. chinese hamster ovary (cho) cells are the workhorse used for more than 60% of industrial therapeutic antibody production. glycoprotein secretion by cho cells requires intracellular processes including transcription, translation, glycosylation, and secretion. within these intracellular steps, post-translational processes are rate limiting for product ... | 2016 | 27252063 |
| effect of ionic liquids on zebrafish (danio rerio) viability, behavior, and histology; correlation between toxicity and ionic liquid aggregation. | the effect of 11 common amidinium, imidazolium, and phosphonium based ionic liquids (ils) on zebrafish (danio rerio) and chinese hamster ovary cells (cho) was investigated with specific emphasis on the effect of anion and cation chain length and aggregation of phosphonium based ils. viability and behavioral alteration in the locomotor activity and place preference, after il treatment of 5 days postfertilization larvae, was recorded. behavior and histological damage evaluation was performed for a ... | 2016 | 27253865 |
| generation of stable chinese hamster ovary pools yielding antibody titers of up to 7.6 g/l using the piggybac transposon system. | chinese hamster ovary (cho) cells remain the default production host for many biopharmaceutical drugs, particularly monoclonal antibodies (mab). production of gram and kilogram quantities of protein typically requires the generation of stable cho clones. unfortunately, this process takes several months, significantly slowing down the drug discovery and development process. therefore, improved technologies are needed to accelerate biopharmaceutical drug discovery and final drug substance manufact ... | 2016 | 27254818 |
| [anti-hbs persistence following primary vaccination with three doses of hepatitis b vaccine among normal and high-responder adults: a 3-year follow-up study]. | to assess the 3-year anti-hbs persistence after primary vaccination with three-dose of hepatitis b vaccine (hepb) among normal and high-responder adults. | 2016 | 27256725 |
| [antibody persistence following primary vaccination with hepatitis b vaccine among normal and high-responder adults: a 5-year follow-up study]. | to evaluate the 5-year antibody persistence and the risk factors associated with the persistence after primary vaccination of hepatitis b vaccine (hepb) among normal or high-response adults. | 2016 | 27256726 |
| [anti-hbs persistence following revaccination with three doses of hepatitis b vaccine among low-responsive adults after primary vaccination: a 4-year follow-up study]. | to assess the 4-year anti-hbs persistence after revaccination with 3-dose of hepatitis b vaccine (hepb) among low-responsive adults. | 2016 | 27256727 |
| [anti-hbs persistence after revaccination with three doses of hepatitis b vaccine among non-responsive adults: a 4-year of follow-up study]. | to explore anti-hbs persistence four years after revaccination with hepatitis b vaccine (hepb) among adults who were non-responsive to hepb primary immunization. | 2016 | 27256728 |
| removal process of prion and parvovirus from human platelet lysates used as clinical-grade supplement for ex vivo cell expansion. | pooled human platelet lysate (hpl) is becoming the new gold standard as supplement for ex vivo cell culture for clinical protocols. however, the risk of pathogen contamination of hpl increases with the platelet pool size. we hypothesized that hollow fiber anion exchange membrane chromatography using qyuspeed d (qsd) could remove resistant and untested bloodborne pathogens, such as parvoviruses and prions, from hpl-supplemented growth media without substantially affecting their capacity to suppor ... | 2016 | 27260210 |
| [effect of intron orientation on the expression of transgene imposed by mar expression vector in stably recombinant cho cells]. | to determine the effect of intron orientation on the transgene expression level imposed by matrix attachment region (mar) expression vector. | 2016 | 27263292 |
| corrigendum to "characterization of recombinant human diamine oxidase (rhdao) produced in chinese hamster ovary (cho) cells" [j. biotechnol. 227 (2016) 120-130]. | 2016 | 27268045 | |
| highly efficient transfer of chromosomes to a broad range of target cells using chinese hamster ovary cells expressing murine leukemia virus-derived envelope proteins. | microcell-mediated chromosome transfer (mmct) is an essential step for introducing chromosomes from donor cells to recipient cells. mmct allows not only for genetic/epigenetic analysis of specific chromosomes, but also for utilization of human and mouse artificial chromosomes (hacs/macs) as gene delivery vectors. although the scientific demand for genome scale analyses is increasing, the poor transfer efficiency of the current method has hampered the application of chromosome engineering technol ... | 2016 | 27271046 |
| targeted delivery of epirubicin to cancer cells by polyvalent aptamer system in vitro and in vivo. | the clinical use of epirubicin (epi), as an anthracycline drug, is limited because of its cardiotoxicity. here, an epirubicin (epi)-modified polyvalent aptamer system (mpas) conjugate was developed for the treatment of both murine colon carcinoma cells (c26) and breast cancer cells (mcf-7). | 2016 | 27283831 |
| rabbits immunized with epstein-barr virus gh/gl or gb recombinant proteins elicit higher serum virus neutralizing activity than gp350. | epstein-barr virus (ebv) is the primary cause of infectious mononucleosis and has been strongly implicated in the etiology of multiple epithelial and lymphoid cancers, such as nasopharyngeal carcinoma, gastric carcinoma, hodgkin lymphoma, burkitt lymphoma, non-hodgkin lymphoma and post-transplant lymphoproliferative disorder. there is currently no licensed prophylactic vaccine for ebv. most efforts to develop prophylactic vaccines have focused on ebv gp350, which binds to cd21/cd35 to gain entry ... | 2016 | 27291087 |
| graphene quantum dots from graphite by liquid exfoliation showing excitation-independent emission, fluorescence upconversion and delayed fluorescence. | facile synthesis of 2-10 nm-sized graphene quantum dots (gqds) from graphite powder by organic solvent-assisted liquid exfoliation using a sonochemical method is reported in this study. synthesized gqds are well dispersed in organic solvents like ethyl acetoacetate (eaa), dimethyl formamide (dmf) and also in water. maldi-tof mass spectrometry reveals its selective mass fragmentation. detailed characterizations by various techniques like x-ray diffraction (xrd), fourier transform infrared spectro ... | 2016 | 27302411 |
| multigene expression in stable cho cell pools generated with the piggybac transposon system. | heterogenous populations of recombinant cells (cell pools) stably expressing 1-4 transgenes were generated from chinese hamster overy (cho) cells with the piggybac (pb) transposon system. the cell pools produced different combinations of three model proteins-enhanced green fluorescent protein (egfp), secreted alkaline phosphatase (seap), and a monoclonal igg1 antibody. each transgene was present on a separate pb donor plasmid with either the same or a different selection gene. in both cases, we ... | 2016 | 27302570 |
| predominant expression of hybrid n-glycans has distinct cellular roles relative to complex and oligomannose n-glycans. | glycosylation modulates growth, maintenance, and stress signaling processes. consequently, altered n-glycosylation is associated with reduced fitness and disease. therefore, expanding our understanding of n-glycans in altering biological processes is of utmost interest. herein, clustered regularly interspaced short palindromic repeats/caspase9 (crispr/cas9) technology was employed to engineer a glycosylation mutant chinese hamster ovary (cho) cell line, k16, which expresses predominantly hybrid ... | 2016 | 27304954 |
| use of in vitro assays to assess the potential cytotoxic, genotoxic and antigenotoxic effects of vanillic and cinnamic acid. | vanillic acid (va) found in vanilla and cinnamic acid (ca) the precursor of flavonoids and found in cinnamon oil, are natural plant phenolic acids which are secondary aromatic plant products suggested to possess many physiological and pharmacological functions. in vitro and in vivo experiments have shown that phenolic acids exhibit powerful effects on biological responses by scavenging free radicals and eliciting antioxidant capacity. in the present study, we investigated the antioxidant capacit ... | 2017 | 27309403 |
| understanding and controlling sialylation in a cho fc-fusion process. | a chinese hamster ovary (cho) bioprocess, where the product is a sialylated fc-fusion protein, was operated at pilot and manufacturing scale and significant variation of sialylation level was observed. in order to more tightly control glycosylation profiles, we sought to identify the cause of variability. untargeted metabolomics and transcriptomics methods were applied to select samples from the large scale runs. lower sialylation was correlated with elevated mannose levels, a shift in glucose m ... | 2016 | 27310468 |
| dc-sign and l-sign are attachment factors that promote infection of target cells by human metapneumovirus in the presence or absence of cellular glycosaminoglycans. | it is well established that glycosaminoglycans (gags) function as attachment factors for human metapneumovirus (hmpv), concentrating virions at the cell surface to promote interaction with other receptors for virus entry and infection. there is increasing evidence to suggest that multiple receptors may exhibit the capacity to promote infectious entry of hmpv into host cells; however, definitive identification of specific transmembrane receptors for hmpv attachment and entry is complicated by the ... | 2016 | 27334579 |
| genotoxicity biomarkers for airborne particulate matter (pm2.5) in an area under petrochemical influence. | the effects of fine inhalable particles (pm2.5) were evaluated in an area under the influence of a petrochemical industry, investigating the sensitivity of different genotoxicity biomarkers. organic extracts were obtained from pm2.5 samples at two sites, positioned in the first and second preferential wind direction in the area. the extracts were evaluated with salmonella/microsome assay, microsuspension method, strains ta98, yg1021 and yg1024. the mammalian metabolization fraction (s9) was used ... | 2016 | 27343868 |
| a theoretical estimate for nucleotide sugar demand towards chinese hamster ovary cellular glycosylation. | glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rtps). in order to define optimal cell culture feeding strategies that control rtp glycosylation, it is necessary to know how nucleotide sugars (nss) are consumed towards host cell and rtp glycosylation. here, we present a theoretical framework that integrates the reported glycoproteome of cho cells, the number of n-linked and o-galnac glycosylation sites on individual host c ... | 2016 | 27345611 |
| design, synthesis, and in vitro antituberculosis activity of 2(5h)-furanone derivatives. | a series of 2(5h)-furanone-based compounds were synthesized from commercially available mucohalic acids. from the first-generation compounds, three showed inhibitory activity (10 µg/ml) of at least 35% against mycobacterium smegmatis mc(2) 155 growth (bioscreen c system). in screening the active first-generation compounds for growth inhibition against mycobacterium tuberculosis h37rv, the most active compound was identified with a minimum inhibitory concentration (mic99 ) of 8.07 µg/ml (15.8 µm) ... | 2016 | 27346745 |
| effects of heterocyclic-based head group modifications on the structure-activity relationship of tocopherol-based lipids for non-viral gene delivery. | gene therapy, a promising strategy for the delivery of therapeutic nucleic acids, is greatly dependent on the development of efficient vectors. in this study, we designed and synthesized several tocopherol-based lipids varying in the head group region. here, we present the structure-activity relationship of stable aqueous suspensions of lipids that were synthetically prepared and formulated with 1,2-dioleoyl phosphatidyl ethanolamine (dope) as the co-lipid. the physicochemical properties such as ... | 2016 | 27348545 |
| subchronic and genetic safety evaluation of a calcium fructoborate in rats. | a branded calcium fructoborate product, a nature-identical calcium salt of bis (fructose) ester of boric acid found in plants and a natural source of boron in the human diet and sold under the trade name fruitex-b(®) brand calcium fructoborate ("frxb"), was evaluated in a 90-day dietary toxicity study and two genotoxicity studies. in the 90-day study, four groups of 10 male and 10 female crl:sd cd(®) igs rats were fed diets with frxb admixtures of 0.56, 1.12, and 1.68% dietary concentration, pro ... | 2016 | 27350145 |
| the infectious bac genomic dna expression library: a high capacity vector system for functional genomics. | gene dosage plays a critical role in a range of cellular phenotypes, yet most cellular expression systems use heterologous cdna-based vectors which express proteins well above physiological levels. in contrast, genomic dna expression vectors generate physiologically-relevant levels of gene expression by carrying the whole genomic dna locus of a gene including its regulatory elements. here we describe the first genomic dna expression library generated using the high-capacity herpes simplex virus- ... | 2016 | 27353647 |
| [domestication of suspension cho cells and its application in the expression of anti-psma antibody]. | to domesticate adherent chinese hamster ovary (cho) cells into suspension cho cells (cho-s) cultured in serum-free medium,and evaluate the application of the cho-s cells in antibody expression. | 2016 | 27358992 |
| percutaneous treatment of herniated lumbar discs with ozone: investigation of the mechanisms of action. | to elucidate the mechanism of action of intradiscal oxygen-ozone therapy for herniated intervertebral disc therapy. | 2016 | 27363296 |
| augmenting chinese hamster genome assembly by identifying regions of high confidence. | chinese hamster ovary (cho) cell lines are the dominant industrial workhorses for therapeutic recombinant protein production. the availability of genome sequence of chinese hamster and cho cells will spur further genome and rna sequencing of producing cell lines. however, the mammalian genomes assembled using shot-gun sequencing data still contain regions of uncertain quality due to assembly errors. identifying high confidence regions in the assembled genome will facilitate its use for cell engi ... | 2016 | 27374913 |
| pingyangmycin and bleomycin share the same cytotoxicity pathway. | pingyangmycin is an anticancer drug known as bleomycin a5 (a5), discovered in the pingyang county of zhejiang province of china. bleomycin (blm) is a mixture of mainly two compounds (a2 and b2), which is on the world health organization's list of essential medicines. both blm and a5 are hydrophilic molecules that depend on transporters or endocytosis receptors to get inside of cells. once inside, the anticancer activities rely on their abilities to produce dna breaks, thus leading to cell death. ... | 2016 | 27376254 |
| heparin: past, present, and future. | heparin, the most widely used anticoagulant drug in the world today, remains an animal-derived product with the attendant risks of adulteration and contamination. a contamination crisis in 2007-2008 increased the impetus to provide non-animal-derived sources of heparin, produced under cgmp conditions. in addition, recent studies suggest that heparin may have significant antineoplastic activity, separate and distinct from its anticoagulant activity, while other studies indicate a role for heparin ... | 2016 | 27384570 |
| exposure to sirna-galnac conjugates in systems of the standard test battery for genotoxicity. | registration of pharmaceuticals requires an assessment of their genotoxic potential using in vitro and in vivo tests outlined in the international conference on harmonisation (ich) guidance s2(r1). we have evaluated numerous sirna-n-acetylgalactosamine (galnac) conjugates containing phosphorothioate linkages and various combinations of 2'-fluoro and 2'-o-methyl ribose modifications of multiple nucleotides in the ich battery of assays, all of which have uniformly yielded negative results. to veri ... | 2016 | 27387336 |
| exploring the capabilities of fluorometric online monitoring on chinese hamster ovary cell cultivations producing a monoclonal antibody. | online monitoring of chinese hamster ovary fed-batch cell cultures via two-dimensional fluorescence spectroscopy (2dfs) was evaluated in this work. particular attention was directed toward different process strategies regarding the use of nutrient-rich feed media and temperature shifts. these intentionally performed process manipulations broadened the variances in the obtained fluorescence spectra and this was suspected to hamper the generation of reliable soft sensors. principal component analy ... | 2016 | 27390298 |
| over-expressed human trek-1 inhibits cho cell proliferation via inhibiting pka and p38 mapk pathways and subsequently inducing g1 arrest. | recent studies have shown that the two-pore-domain potassium channel trek-1 is involved in the proliferation of neural stem cells, astrocytes and human osteoblasts. in this study, we investigated how trek-1 affected the proliferation of chinese hamster ovary (cho) cells in vitro. | 2016 | 27397543 |
| characterization of neuropeptide b (npb), neuropeptide w (npw), and their receptors in chickens: evidence for npw being a novel inhibitor of pituitary gh and prolactin secretion. | the 2 structurally and functionally related peptides, neuropeptide b (npb) and neuropeptide w (npw), together with their receptor(s) (npbwr1/npbwr2) constitute the npb/npw system, which acts mainly on the central nervous system to regulate many physiological processes in mammals. however, little is known about this npb/npw system in nonmammalian vertebrates. in this study, the functionality and expression of this npb/npw system and its actions on the pituitary were investigated in chickens. the ... | 2016 | 27399877 |