Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| stenotrophomonas, achromobacter, and nonmelioid burkholderia species: antimicrobial resistance and therapeutic strategies. | stenotrophomonas maltophilia, achromobacter xylosoxidans, and nonmelioid burkholderia species, namely, burkholderia cepacia complex, collectively are a group of troublesome nonfermenters. although not inherently virulent organisms, these environmental gram negatives can complicate treatment in those who are immunocompromised, critically ill in the intensive care unit and those patients with suppurative lung disease, such as cystic fibrosis. through a range of intrinsic antimicrobial resistance m ... | 2015 | 25643274 |
| stenotrophomonas maltophilia in lower respiratory tract infections. | stenotrophomonas maltophilia infection is gaining importance as an important cause of nosocomial pneumonia due to its characteristic inherent resistance to many broad- spectrum antibiotics. in this study we evaluated the demographic, clinical and microbiological profile of patients with lower respiratory tract infection due to stenotrophomonas maltophilia. | 2014 | 25653948 |
| identification and characterization of a serious multidrug resistant stenotrophomonas maltophilia strain in china. | an s. maltophilia strain named wj66 was isolated from a patient; wj66 showed resistance to more antibiotics than the other s. maltophilia strains. this bacteraemia is resistant to sulphonamides, or fluoroquinolones, while the representative strain of s. maltophilia, k279a, is sensitive to both. to explore drug resistance determinants of this strain, the draft genome sequence of wj66 was determined and compared to other s. maltophilia sequences. genome sequencing and genome-wide evolutionary anal ... | 2015 | 25654114 |
| the optimization of fermentation conditions and enzyme properties of stenotrophomonas maltophilia for protease production. | intestinal bacteria play a significant physiological role in silkworms. proteases secreted by intestinal microbes can promote the digestion of the nutrient by bombyx mori and the absorption of mulberry leaves. intestinal bacteria from jingsong × haoyue in the fourth larvae were isolated and purified to obtain high activity protease-producing bacteria. the morphology of the identified bacterial colony was examined by microscopy combined with the 16s rdna method. the results showed that this bacte ... | 2017 | 25656812 |
| stenotrophomonas maltophilia endophthalmitis caused by surgical equipment contamination: an emerging nosocomial infection. | we report three cases of stenotrophomonas maltophilia endophthalmitis after uneventful extracapsular cataract extraction with intraocular lens implantation-related to surgical equipment contamination. | 2017 | 25667741 |
| purification, characterization, and gene cloning of a chitinase from stenotrophomonas maltophilia n4. | the stenotrophomonas maltophilia synthesises high-activity chitinase in response to chitin or chitosan induction. the enzyme was purified 8.5 fold and subjected to characterisation. the optimum hydrolysis conditions for this enzyme when using colloidal chitin as substrate were ph 5.6 and temperature of 45 °c. the enzyme demonstrated high thermal stability at 45 °c within 2 h. the studied chitinase exhibited high activity towards colloidal chitin, glycol chitin and chitosan, while it did not hydr ... | 2015 | 25684706 |
| diversity of bacteria nesting the plant cover of north sinai deserts, egypt. | north sinai deserts were surveyed for the predominant plant cover and for the culturable bacteria nesting their roots and shoots. among 43 plant species reported, 13 are perennial (e.g. fagonia spp., pancratium spp.) and 30 annuals (e.g. bromus spp., erodium spp.). eleven species possessed rhizo-sheath, e.g. cyperus capitatus, panicum turgidum and trisetaria koelerioides. microbiological analyses demonstrated: the great diversity and richness of associated culturable bacteria, in particular nitr ... | 2012 | 25685397 |
| isolation of vermamoeba vermiformis and associated bacteria in hospital water. | to detect new potential pathogens in hospital water, we isolated free-living amoebae in water samples taken from three different hospitals in marseille (france). the samples were inoculated in media containing saline buffer and various bacteria as nutrient sources. the isolated amoebae were identified by gene sequencing. among the 105 water samples, taken from 19 sites, we isolated 14 amoebae, of which 9 vermamoeba vermiformis and 5 acanthamoeba sp. none of the amoebae showed the presence of obl ... | 2015 | 25697664 |
| draft genome sequence of stenotrophomonas maltophilia strain b418, a promising agent for biocontrol of plant pathogens and root-knot nematode. | stenotrophomonas maltophilia strain b418 was isolated from a barley rhizosphere in china. this bacterium exhibits broad-spectrum inhibitory activities against plant pathogens and root-knot nematode along with growth-promoting effects. here, we present the draft genome sequence of s. maltophilia b418. | 2015 | 25700397 |
| biotransformation of oleic acid into 10-ketostearic acid by recombinant corynebacterium glutamicum-based biocatalyst. | to produce 10-ketostearic acid from oleic acid. | 2015 | 25700814 |
| [investigation of the presence of class 1, 2, 3 integrons and their relationships with antibiotic resistance in clinical stenotrophomonas maltophilia isolates]. | stenotrophomonas maltophilia is an opportunistic emergent pathogen causing hospital-acquired infections. it is resistant to majority of the broad spectrum antibiotics due to several mechanisms which significantly limit the treatment options. although the relationship between integrons, mobile genetic elements which play role in transferring resistance genes, and the antibiotic resistance in different gram-negative bacteria have been investigated, the data are limited in turkey especially for s.m ... | 2015 | 25706729 |
| multi-channel microfluidic biosensor platform applied for online monitoring and screening of biofilm formation and activity. | bacterial colonization of surfaces and interfaces has a major impact on various areas including biotechnology, medicine, food industries, and water technologies. in most of these areas biofilm development has a strong impact on hygiene situations, product quality, and process efficacies. in consequence, biofilm manipulation and prevention is a fundamental issue to avoid adverse impacts. for such scenario online, non-destructive biofilm monitoring systems become important in many technical and in ... | 2015 | 25706987 |
| microbial contamination of glaucoma eyedrops used by patients compared with ocular medications used in the hospital. | the aim of this study was to compare the percentage of contamination of multiuse eyedrops applied by glaucoma patients at home and by the medical personnel at the outpatient department, the ward, and the operating room of our department of ophthalmology. eyedrops were collected over a period of 11 months. samples were taken from the dropper tip (smear), drops, and the residual fluid inside the bottle and cultivated on blood agar. colony forming units were counted and identified by mass spectrome ... | 2015 | 25715262 |
| late stenotrophomonas maltophilia pacemaker infective endocarditis. | 2015 | 25722039 | |
| the sul1 gene in stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole. | emerging resistance to trimethoprim/sulfamethoxazole (sxt) poses a serious threat to the treatment of stenotrophomonas maltophilia infections. we determined the prevalence and molecular characteristics of acquired sxt resistance in recent clinical s. maltophilia isolates obtained from korea. a total of 252 clinical isolates of s. maltophilia were collected from 10 university hospitals in korea between 2009 and 2010. antimicrobial susceptibility was determined by using the clsi agar dilution meth ... | 2015 | 25729729 |
| characterizing novel thermophilic amylase producing bacteria from taptapani hot spring, odisha, india. | amylases play a vital role in biotechnological studies and rank an important position in the world enzyme market (25% to 33%). bioprocess method of amylase production is more effective than the other sources, since the technique is easy, cost effective, fast, and the enzymes of required properties can be procured. | 2014 | 25741425 |
| high-level quinolone resistance is associated with the overexpression of smevwx in stenotrophomonas maltophilia clinical isolates. | stenotrophomonas maltophilia is the only known bacterium in which quinolone-resistant isolates do not present mutations in the genes encoding bacterial topoisomerases. the expression of the intrinsic quinolone resistance elements smedef, smevwx and smqnr was analysed in 31 clinical s. maltophilia isolates presenting a minimum inhibitory concentration (mic) range to ciprofloxacin between 0.5 and > 32 μg/ml; 11 (35.5%) overexpressed smedef, 2 (6.5%) presenting the highest quinolone mics overexpres ... | 2015 | 25753190 |
| distribution of class 1 integrons, sul1 and sul2 genes among clinical isolates of stenotrophomonas maltophilia from a tertiary care hospital in north india. | stenotrophomonas maltophilia is an emerging nosocomial pathogen responsible for serious human infections. this study was carried out to determine antibiotic susceptibility, resistance mechanisms (integrons, sul1 and sul2), and genetic relatedness (enterobacterial repetitive intergenic consensus [eric]-pcr) among 106 clinical isolates of s. maltophilia from india. twenty-four (22.6%) of s. maltophilia isolates exhibited resistance to mainstay antibiotic trimethoprim-sulfamethoxazole (tmp-smx). ex ... | 2015 | 25781206 |
| antibiogram of stenotrophomonas maltophilia isolated from nkonkobe municipality, eastern cape province, south africa. | assessment of resistance genes is imperative, as they become disseminated to bacterial flora in plants and to the indigenous bacterial community, and thus ultimately contributes to the clinical problems of antibiotic resistant pathogens. | 2015 | 25789125 |
| stenotrophomonas maltophilia: complicating treatment of esbl uti. | stenotrophomonas maltophilia (s. maltophilia) is a gram-negative bacillus emerging as an opportunistic, nosocomial pathogen associated with a high mortality rate. the organism has been shown to survive several biocides used in the hospital setting. hospital water sources can serve as a reservoir for s. maltophilia. the transmission of s. maltophilia to susceptible individuals may occur through direct contact with the source or through the hands of health care personnel. s. maltophilia is usually ... | 2015 | 25789262 |
| quantitative analysis of volatile metabolites released in vitro by bacteria of the genus stenotrophomonas for identification of breath biomarkers of respiratory infection in cystic fibrosis. | the aim of the present study was to characterize the volatile metabolites produced by genotypically diverse strains of the stenotrophomonas genus in order to evaluate their potential as biomarkers of lung infection by non-invasive breath analysis. volatile organic compounds (vocs) emitted from 15 clinical and five environmental strains belonging to different genogroups of stenotrophomonas maltophilia (n = 18) and stenotrophomonas rhizophila (n = 2) cultured in mueller-hinton broth (mhb) liquid m ... | 2015 | 25830686 |
| development and validation of an hplc-uv assay to quantify plasma levels of sulfametrol: a preferential antibiotic in children. | sulfonamides in combination with trimethoprim are frequently used antibiotics. they work synergistic. in infections with pneumocystis jiroveci or stenotrophomonas maltophilia, higher dosages are indicated than in other infections. therapeutic drug monitoring (tdm) is warranted to assure the efficacy while limiting toxicity. although trimethoprim in combination with sulfamethoxazole is the most common combination with established tdm target concentrations, the intravenous formulation is not suite ... | 2015 | 25830929 |
| use of loop-mediated isothermal amplification to detect six groups of pathogens causing secondary lower respiratory bacterial infections in horses. | microbial substitution occasionally occurs following the administration of antimicrobials to horses that have pneumonia or pleuropneumonia. four specific loop-mediated isothermal amplification (lamp) assays were developed to detect some equine respiratory pathogens, namely strains of the bacteroides-prevotella group, klebsiella pneumoniae, stenotrophomonas maltophilia, and staphylococcus aureus. these four lamp assays and two previously published lamp assays targeting escherichia coli or pseudom ... | 2015 | 25846404 |
| increased prevalence and resistance of important pathogens recovered from respiratory specimens of cystic fibrosis patients during a decade. | the study objective was to identify changes of prevalence and resistance of important pathogens in specimens of cystic fibrosis (cf) patients within a decade. | 2015 | 25851069 |
| pan-resistant achromobacter xylosoxidans and stenotrophomonas maltophilia infection in cystic fibrosis does not reduce survival after lung transplantation. | the number of cystic fibrosis (cf) patients undergoing lung transplantation continues to grow, as does the prevalence of multidrug-resistant (mdr) gram-negative rods. however, the posttransplant survival of patients with mdr pathogens, specifically pan-resistant achromobacter xylosoxidans and stenotrophomonas maltophilia, is poorly characterized. | 2015 | 25856407 |
| detection and location of op-degrading activity: a model to integrate education and research. | the environmental sampling research module (esrm) is an investigative/discovery module that provides undergraduate research experiences for students as part of an interdisciplinary research-based biotechnology curriculum at the university of houston campus. as part of the esrm, students collect soil samples from various locations to test for the presence of organophosphorous (op) degrading bacteria. at the end of this research project students submit a research paper on their field and laborator ... | 2015 | 25863354 |
| friends or foes: can we make a distinction between beneficial and harmful strains of the stenotrophomonas maltophilia complex? | stenotrophomonas maltophilia is an emerging multi-drug-resistant global opportunistic pathogen of environmental, mainly plant-associated origin. it is also used as a biocontrol or stress protecting agent for crops in sustainable agricultural as well as in bioremediation strategies. in order to establish effective protocols to distinguish harmless from harmful strains, our discussion must take into consideration the current data available surrounding the ecology, evolution and pathogenicity of th ... | 2015 | 25873912 |
| emerging bacterial pathogens and changing concepts of bacterial pathogenesis in cystic fibrosis. | chronic suppurative lower airway infection is a hallmark feature of cystic fibrosis (cf). decades of experience in clinical microbiology have enabled the development of improved technologies and approaches for the cultivation and identification of microorganisms from sputum. it is increasingly apparent that the microbial constituents of the lower airways in cf exist in a dynamic state. indeed, while changes in prevalence of various pathogens occur through ageing, differences exist in successive ... | 2015 | 25881770 |
| draft genome sequence of the biofilm-forming stenotrophomonas maltophilia strain 53. | a clinical strain of stenotrophomonas maltophilia (designated strain 53) was obtained, and a whole-genome sequence was generated. the subsequent draft whole-genome sequence demonstrated the presence of a number of genes encoding for proteins involved in resistance to a number of antimicrobial therapies. | 2015 | 25883296 |
| stenotrophomonas maltophilia bloodstream infection in patients with hematologic malignancies: a retrospective study and in vitro activities of antimicrobial combinations. | stenotrophomonas maltophilia causes serious infections in immunocompromised hosts. here, we analyzed the clinical characteristics of s. maltophilia bloodstream infection (bsi) in patients with hematologic malignancies and evaluated in vitro synergistic effects of antimicrobial combinations. | 2015 | 25887489 |
| a rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water. | water and high purity water (hpw) distribution systems can be contaminated with human pathogenic microorganisms. this biocontamination may pose a risk to human health as hpw is commonly used in the industrial, pharmaceutical and clinical sectors. currently, routine microbiological testing of hpw is performed using slow and labour intensive traditional microbiological based techniques. there is a need to develop a rapid culture independent methodology to quantitatively detect and identify biocont ... | 2015 | 25888294 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |
| regulation by soxr of mfsa, which encodes a major facilitator protein involved in paraquat resistance in stenotrophomonas maltophilia. | stenotrophomonas maltophilia mfsa (smlt1083) is an efflux pump in the major facilitator superfamily (mfs). deletion of mfsa renders the strain more susceptible to paraquat, but no alteration in the susceptibility levels of other oxidants is observed. the expression of mfsa is inducible upon challenge with redox cycling/superoxide-generating drug (paraquat, menadione and plumbagin) treatments and is directly regulated by soxr, which is a transcription regulator and sensor of superoxide-generating ... | 2015 | 25915643 |
| the smeyz efflux pump of stenotrophomonas maltophilia contributes to drug resistance, virulence-related characteristics, and virulence in mice. | the resistance-nodulation-division (rnd)-type efflux pump is one of the causes of the multidrug resistance of stenotrophomonas maltophilia. the roles of the rnd-type efflux pump in physiological functions and virulence, in addition to antibiotic extrusion, have attracted much attention. in this study, the contributions of the constitutively expressed smeyz efflux pump to drug resistance, virulence-related characteristics, and virulence were evaluated. s. maltophilia kj is a clinical isolate of m ... | 2015 | 25918140 |
| the efflux pump smedef contributes to trimethoprim-sulfamethoxazole resistance in stenotrophomonas maltophilia. | trimethoprim-sulfamethoxazole (co-trimoxazole) is one of the antimicrobials of choice for the treatment of stenotrophomonas maltophilia infections. the analysis of mutants either lacking or overexpressing the efflux pump smedef shows that this efflux pump contributes to intrinsic and acquired co-trimoxazole resistance in s. maltophilia. since smedef can extrude a variety of antibiotics, selection with such antimicrobials, including quinolones, might also select for s. maltophilia co-trimoxazole ... | 2015 | 25918144 |
| the identification of stenotrophomonas maltophilia contamination in ultrasound endoscopes and reproduction of decontamination failure by deliberate soiling tests. | an outbreak of stenotrophomonas maltophilia was observed in the cultures of bronchial aspirations. after systematic culturing of the scopes and reprocessing the equipment, it turned out to be a pseudo-outbreak caused by a failure of the disinfector to sterilize ultrasound scopes, subsequently resulting in cross infection of the bronchoscopes via the connecting tubes in the dryer. | 2015 | 25925975 |
| the effect of imipenem and diffusible signaling factors on the secretion of outer membrane vesicles and associated ax21 proteins in stenotrophomonas maltophilia. | outer membrane vesicles (omvs) are small nanoscale structures that are secreted by bacteria and that can carry nucleic acids, proteins, and small metabolites. they can mediate intracellular communication and play a role in virulence. in this study, we show that treatment with the β-lactam antibiotic imipenem leads to a dramatic increase in the secretion of outer membrane vesicles in the nosocomial pathogen stenotrophomonas maltophilia. proteomic analysis of their protein content demonstrated tha ... | 2015 | 25926824 |
| severe ovarian hyperstimulation syndrome complicated by stenotrophomonas maltophilia peritonitis: a case report and literature review. | ovarian hyperstimulation syndrome (ohss) is an iatrogenic disorder resulting from ovulation induction. although the occurrence of this disorder is rare, it can be potentially life-threatening in its most severe forms. we herein present the case of a young nulliparous woman who presented with features of abdominal compartment syndrome and was subsequently diagnosed with severe ohss. all physicians, in particular critical care doctors, must be aware of this rare, but potentially life-threatening i ... | 2015 | 25948367 |
| analysis of microbial load on surgical instruments after clinical use and following manual and automated cleaning. | we aimed to monitor the microbial load and identify the microorganisms recovered from surgical instruments after clinical use and following manual and automated cleaning. | 2015 | 25952049 |
| degradation of abamectin by newly isolated stenotrophomonas maltophilia zjb-14120 and characterization of its abamectin-tolerance mechanism. | an abamectin (abm)-degrading bacterium, stenotrophomonas maltophilia zjb-14120, was isolated and identified. this strain is capable of degrading 84.82% of abm at an initial concentration of 200 mg/l over a 48 h incubation period. this strain showed efficient biodegradation ability (7.81 mg/l/h) to abm and high tolerance (1000 mg/l) to all macrolides tested. in addition to abm, emamectin, erythromycin and spiramycin can also be degraded by this strain. modifications involving either reduction of ... | 2015 | 25957243 |
| inconclusive diagnosis of cystic fibrosis after newborn screening. | to prospectively study infants with an inconclusive diagnosis of cystic fibrosis (cf) identified by newborn screening (nbs; "cf screen positive, inconclusive diagnosis" [cfspid]) for disease manifestations. | 2015 | 25963003 |
| resistance of stenotrophomonas maltophilia to fluoroquinolones: prevalence in a university hospital and possible mechanisms. | the purpose of this study was to investigate the clinical distribution and genotyping of stenotrophomonas maltophilia, its resistance to antimicrobial agents, and the possible mechanisms of this drug resistance. | 2015 | 25985315 |
| haemorrhagic pneumonia caused by stenotrophomonas maltophilia in two newborns. | invasive procedures and antibiotic treatment increase the risk of nosocomial infections in neonatal intensive care units. early identification and appropriate treatment is important. herein we report two cases of massive hemorrhagic pneumonia caused by stenotrophomonas maltophilia. the first case was diagnosed with congenital pneumonia; a chest tube was inserted because of pneumothorax on the third day of life. the second case had been referred with respiratory distress syndrome, and bilateral p ... | 2015 | 25989175 |
| discrepancy in maldi-tof ms identification of uncommon gram-negative bacteria from lower respiratory secretions in patients with cystic fibrosis. | early identification of microbial organisms from respiratory secretions of patients with cystic fibrosis (cf) is important to guide therapeutic decisions. the objective was to compare the accuracy of matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) relative to the conventional phenotypic method in identifying common bacterial isolates, including nonfermenting gram-negative bacteria, in a cohort of patients with cf. | 2015 | 25995646 |
| sensitive, resistant and multi-drug resistant acinetobacter baumanii at saudi arabia hospital eastern region. | since the physicians start use of antibiotics long ago with un-notice drug resistance. however actual problem was recognized about 85 years ago. antibiotic resistant and multi-drug resistant bacterial strains are at rise throughout the world. it is physicians and researchers to take scientific research based appropriate action to overcome this ever-spreading problem. this study is designed to find out sensitive (s), resistant (r) and multi-drug resistant (mdr) acinetobacter baumanii strain along ... | 2015 | 26004714 |
| stenotrophomonas maltophilia keratitis treated with trimethoprim-sulfamethoxazole. | an 84 year-old woman with persistent epithelial defect and a dense stromal infiltrate post-corneal transplantation. according to the microbiological results, it was due to a stenotrophomonas maltophilia (s. maltophilia) resistant to all antibiotics except trimethoprim-sulfamethoxazole (tmp/smx). healing was achieved after three weeks of treatment with oral and topical tmp/smx. | 2015 | 26008926 |
| evaluation of the quality of reprocessing of gastrointestinal endoscopes. | to evaluate the quality of gastrointestinal endoscope reprocessing and discuss the advantages of microbiological surveillance testing of these endoscopes. | 2015 | 26022365 |
| stenotrophomonas maltophilia responds to exogenous ahl signals through the luxr solo smor (smlt1839). | quorum sensing (qs) mediated by acyl homoserine lactone (ahl) molecules are probably the most widespread and studied among gram-negative bacteria. canonical ahl systems are composed by a synthase (luxi family) and a regulator element (luxr family), whose genes are usually adjacent in the genome. however, incomplete ahl-qs machinery lacking the synthase luxi is frequently observed in proteobacteria, and the regulator element is then referred as luxr solo. it has been shown that certain luxr solos ... | 2015 | 26029670 |
| a novel salt-tolerant chitobiosidase discovered by genetic screening of a metagenomic library derived from chitin-amended agricultural soil. | here, we report on the construction of a metagenomic library from a chitin-amended disease-suppressive agricultural soil and its screening for genes that encode novel chitinolytic enzymes. the library, constructed in fosmids in an escherichia coli host, comprised 145,000 clones containing inserts of sizes of 21 to 40 kb, yielding a total of approximately 5.8 gb of cloned soil dna. using genetic screenings by repeated pcr cycles aimed to detect gene sequences of the bacterial chitinase a-class (h ... | 2015 | 26040993 |
| risk factors for lung function decline in a large cohort of young cystic fibrosis patients. | to identify novel risk factors and corroborate previously identified risk factors for mean annual decline in fev1% predicted in a large, contemporary, united states cohort of young cystic fibrosis (cf) patients. | 2015 | 26061914 |
| draft genome sequence of stenotrophomonas maltophilia strain uv74 reveals extensive variability within its genomic group. | we report the draft genome sequence of stenotrophomonas maltophilia uv74, isolated from a vascular ulcer. this draft genome sequence shall contribute to the understanding of the evolution and pathogenicity of this species, particularly regarding isolates of clinical origin. | 2015 | 26067959 |
| real-time monitoring of antimicrobial use density to reduce antimicrobial resistance through the promotion of antimicrobial heterogeneity in a haematology/oncology unit. | in haematology/oncology units, the frequent and heavy use of broad-spectrum antimicrobials can lead to outbreaks of antimicrobial resistance. increasing antimicrobial heterogeneity might be a useful strategy for preventing such resistance. | 2015 | 26071518 |
| rahnella aquatilis sepsis in a premature newborn. | rahnella aquatilis is an infrequently isolated gram-negative rod within the enterobacteriaceae family. the organism's natural habitat is water. the organism is rarely isolated from clinical specimens and it seldom causes infection in immunocompetent individuals. here we present a one-month-old boy who was born prematurely at 27th week of gestation by cesarean section with a birth weight of 730 g. he developed sepsis caused by rahnella aquatilis during the treatment for ventilator associated pneu ... | 2015 | 26090257 |
| prevalence of smqnr and plasmid-mediated quinolone resistance determinants in clinical isolates of stenotrophomonas maltophilia from japan: novel variants of smqnr. | stenotrophomonas maltophilia is an important pathogen in healthcare-associated infections. s. maltophilia may contain smqnr, a quinolone resistance gene encoding the pentapeptide repeat protein, which confers low-level quinolone resistance upon expression in a heterologous host. we investigated the prevalence of smqnr and plasmid-mediated quinolone resistance (pmqr) determinants in s. maltophilia isolates from japan. a total of 181 consecutive and nonduplicate clinical isolates of s. maltophilia ... | 2015 | 26110061 |
| tackling antibiotic resistance in febrile neutropenia: current challenges with and recommendations for managing infections with resistant gram-negative organisms. | multidrug resistant (mdr) gram-negative bacteria (gnb) have emerged as important pathogens and a serious challenge in the management of neutropenic patients worldwide. the great majority of infections are caused by the enterobacteriaceae (especially escherichia coli and klebsiella spp.) and pseudomonas aeruginosa, and less frequently acinetobacter spp. and stenotrophomonas maltophilia. a broader-spectrum empiric antibiotic regimen is usually recommended in patients with a history of prior bloods ... | 2015 | 26115679 |
| biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms. | the present study deals with se(0)- and te(0)-based nanoparticles bio-synthesized by two selenite- and tellurite-reducing bacterial strains, namely stenotrophomonas maltophilia seite02 and ochrobactrum sp. mpv1, isolated from polluted sites. we evidenced that, by regulating culture conditions and exposure time to the selenite and tellurite oxyanions, differently sized zero-valent se and te nanoparticles were produced. the results revealed that these se(0) and te(0) nanoparticles possess antimicr ... | 2015 | 26136728 |
| lung transplantation after endoscopic lung volume reduction. | endoscopic lung volume reduction (elvr) has become an established treatment option in selected patients with end-stage lung emphysema. elvr, however, does not always prevent disease progression, and patients may inevitably be considered for lung transplantation. | 2015 | 26138023 |
| characteristics of stenotrophomonas maltophilia bacteremia in children. | empiric antimicrobial coverage in compromised hosts commonly includes pseudomonal coverage but often lacks coverage against stenotrophomonas maltophilia. identification of risk factors specific for s. maltophilia infection may lead to prompt initiation of appropriate antibiotics and improved outcome. | 2016 | 26139084 |
| a monte carlo pharmacokinetic/pharmacodynamic simulation to evaluate the efficacy of minocycline, tigecycline, moxifloxacin, and levofloxacin in the treatment of hospital-acquired pneumonia caused by stenotrophomonas maltophilia. | stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in recent years. increasing antimicrobial resistance and other contraindications have greatly compromised trimethoprim/sulfamethoxazole (sxt) as the first-line therapeutic option. the objective of this study was to explore other options for treating hospital-acquired pneumonia (hap) caused by s. maltophilia. | 2015 | 26167850 |
| type ii secretion-dependent degradative and cytotoxic activities mediated by stenotrophomonas maltophilia serine proteases stmpr1 and stmpr2. | stenotrophomonas maltophilia is an emerging opportunistic pathogen that primarily causes pneumonia and bacteremia in immunocompromised individuals. we recently reported that s. maltophilia strain k279a encodes the xps type ii secretion system and that xps promotes rounding, actin rearrangement, detachment, and death in the human lung epithelial cell line a549. here, we show that xps-dependent cell rounding and detachment occur with multiple human and murine cell lines and that serine protease in ... | 2015 | 26169274 |
| regulation of smqnr expression by smqnrr is strain-specific in stenotrophomonas maltophilia. | 2015 | 26174722 | |
| antibiotic resistance in the opportunistic pathogen stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an environmental bacterium found in the soil, associated with plants and animals, and in aquatic environments. it is also an opportunistic pathogen now causing an increasing number of nosocomial infections. the treatment of s. maltophilia is quite difficult given its intrinsic resistance to a number of antibiotics, and because it is able to acquire new resistances via horizontal gene transfer and mutations. certainly, strains resistant to quinolones, cotrimoxale a ... | 2015 | 26175724 |
| correction for huang et al., nagz-dependent and nagz-independent mechanisms for β-lactamase expression in stenotrophomonas maltophilia. | 2015 | 26185274 | |
| molecular epidemiology and antimicrobial susceptibility of stenotrophomonas maltophilia in a bulgarian university hospital over a 5-year period (2007-2012). | 2015 | 26198085 | |
| predictive studies suggest that the risk for the selection of antibiotic resistance by biocides is likely low in stenotrophomonas maltophilia. | biocides are used without restriction for several purposes. as a consequence, large amounts of biocides are released without any control in the environment, a situation that can challenge the microbial population dynamics, including selection of antibiotic resistant bacteria. previous work has shown that triclosan selects stenotrophomonas maltophilia antibiotic resistant mutants overexpressing the efflux pump smedef and induces expression of this pump triggering transient low-level resistance. i ... | 2015 | 26201074 |
| changing epidemiology of the respiratory bacteriology of patients with cystic fibrosis. | monitoring potential changes in the epidemiology of cystic fibrosis (cf) pathogens furthers our understanding of the potential impact of interventions. | 2016 | 26203598 |
| risk factors associated with stenotrophomonas maltophilia bacteremia: a matched case-control study. | stenotrophomonas maltophilia is an important nosocomial bacterial pathogen, as is pseudomonas aeruginosa. differentiation of these bacteria as bacteremic agents is critical in the clinical setting and to define a therapeutic strategy; however, the associated factors and prognosis for s. maltophilia bacteremia have not been fully evaluated to adequately characterize these factors. we first conducted a matched case-control study to clarify these questions. a total of 30 case patients with s. malto ... | 2015 | 26208218 |
| functional properties of the major outer membrane protein in stenotrophomonas maltophilia. | stenotrophomonas maltophilia is an opportunistic pathogen that is closely associated with high morbidity and mortality in debilitated and immunocompromised individuals. therefore, to investigate the pathogenesis mechanism is urgently required. however, there are very few studies to evaluate the functional properties of outer membrane protein, which may contribute to the pathogenesis in s. maltophilia. in this study, three abundant proteins in the outer membrane fraction of s. maltophilia were id ... | 2015 | 26224456 |
| in vitro interaction of stenotrophomonas maltophilia with human monocyte-derived dendritic cells. | stenotrophomonas maltophilia is increasingly identified as an opportunistic pathogen in immunocompromised, cancer and cystic fibrosis (cf) patients. knowledge on innate immune responses to s. maltophilia and its potential modulation is poor. the present work investigated the ability of 12 clinical s. maltophilia strains (five from cf patients, seven from non-cf patients) and one environmental strain to survive inside human monocyte-derived dendritic cells (dcs). the effects of the bacteria on ma ... | 2015 | 26236302 |
| mechanisms of antimicrobial resistance in gram-negative bacilli. | the burden of multidrug resistance in gram-negative bacilli (gnb) now represents a daily issue for the management of antimicrobial therapy in intensive care unit (icu) patients. in enterobacteriaceae, the dramatic increase in the rates of resistance to third-generation cephalosporins mainly results from the spread of plasmid-borne extended-spectrum beta-lactamase (esbl), especially those belonging to the ctx-m family. the efficacy of beta-lactam/beta-lactamase inhibitor associations for severe i ... | 2015 | 26261001 |
| comparative genomics of environmental and clinical stenotrophomonas maltophilia strains with different antibiotic resistance profiles. | stenotrophomonas maltophilia, a ubiquitous gram-negative γ-proteobacterium, has emerged as an important opportunistic pathogen responsible for nosocomial infections. a major characteristic of clinical isolates is their high intrinsic or acquired antibiotic resistance level. the aim of this study was to decipher the genetic determinism of antibiotic resistance among strains from different origins (i.e., natural environment and clinical origin) showing various antibiotic resistance profiles. to th ... | 2015 | 26276674 |
| interplay among membrane-bound lytic transglycosylase d1, the crebc two-component regulatory system, the ampng-ampdi-nagz-ampr regulatory circuit, and l1/l2 β-lactamase expression in stenotrophomonas maltophilia. | lytic transglycosylases (lts) are an important class of enzymes involved in peptidoglycan (pg) cleavage, with the concomitant formation of an intramolecular 1,6-anhydromuramoyl reaction product. there are six annotated lt genes in the stenotrophomonas maltophilia genome, including genes for five membrane-bound lts (mlta, mltb1, mltb2, mltd1, and mltd2) and a gene for soluble lt (slt). six lts of s. maltophilia kj were systematically mutated, yielding the δmlta, δmltb1, δmltb2, δmltd1, δmltd2, an ... | 2015 | 26282431 |
| decoding the genetic and functional diversity of the dsf quorum-sensing system in stenotrophomonas maltophilia. | stenotrophomonas maltophilia uses the diffusible signal factor (dsf) quorum sensing (qs) system to mediate intra- and inter-specific signaling and regulate virulence-related processes. the components of this system are encoded by the rpf cluster, with genes rpff and rpfc encoding for the dsf synthase rpff and sensor rpfc, respectively. recently, we have shown that there exist two variants of the rpf cluster (rpf-1 and rpf-2), distinguishing two groups of s. maltophilia strains. surprisingly, onl ... | 2015 | 26284046 |
| whole-genome sequencing identifies emergence of a quinolone resistance mutation in a case of stenotrophomonas maltophilia bacteremia. | whole-genome sequences for stenotrophomonas maltophilia serial isolates from a bacteremic patient before and after development of levofloxacin resistance were assembled de novo and differed by one single-nucleotide variant in smet, a repressor for multidrug efflux operon smedef. along with sequenced isolates from five contemporaneous cases, they displayed considerable diversity compared against all published complete genomes. whole-genome sequencing and complete assembly can conclusively identif ... | 2015 | 26324280 |
| the isolation and characterization of two stenotrophomonas maltophilia bacteriophages capable of cross-taxonomic order infectivity. | a rapid worldwide increase in the number of human infections caused by the extremely antibiotic resistant bacterium stenotrophomonas maltophilia is prompting alarm. one potential treatment solution to the current antibiotic resistance dilemma is "phage therapy", the clinical application of bacteriophages to selectively kill bacteria. | 2015 | 26335566 |
| genetic manipulation of stenotrophomonas maltophilia. | stenotrophomonas maltophilia is a gram-negative, aerobic, motile, environmental bacterium that is emerging as an important nosocomial pathogen with high rates of attributable mortality in severely ill patients. s. maltophilia is of particular concern to patients suffering from cystic fibrosis (cf) as it has been shown to colonize airway epithelial and establish a chronic infection. here we describe several molecular techniques for the genetic manipulation of this bacterium, including dna extract ... | 2015 | 26344220 |
| insights into the degradation of chlorimuron-ethyl by stenotrophomonas maltophilia d310-3. | in this study, the effects of cultivation conditions on the degradation of chlorimuron-ethyl by stenotrophomonas maltophilia d310-3, which exhibits a high chlorimuron-ethyl-degrading capability, were investigated. to improve the biodegradation efficiency, the cultivation conditions were optimized using response surface methodology (rsm) based on box-behnken design (bbd). the maximum biodegradation rate (89.9%) was obtained at the optimal conditions (culture time, 6 d; substrate concentration, 50 ... | 2016 | 26363318 |
| in vitro activity of aminoglycosides against clinical isolates of acinetobacter baumannii complex and other nonfermentative gram-negative bacilli causing healthcare-associated bloodstream infections in taiwan. | aminoglycosides possess in vitro activity against aerobic and facultative gram-negative bacilli. however, nationwide surveillance on susceptibility data of acinetobacter baumannii complex and pseudomonas aeruginosa to aminoglycosides was limited, and aminoglycoside resistance has emerged in the past decade. we study the in vitro susceptibility of a. baumannii complex and other nonfermentative gram-negative bacilli (nfgnb) to aminoglycosides. | 2016 | 26364729 |
| high-rate biological denitrification in the cyclic rotating-bed biological reactor: effect of cod/no3(-), nitrate concentration and salinity and the phylogenetic analysis of denitrifiers. | the effects of cod/no3(-) ratio, nitrate concentration and salinity was tested on the performance of the crbr in denitrification with catechol as carbon source. the maximum nitrate reduction attained at cod/no3(-) ratio of 1. the crbr operated at optimum cod/no3(-) ratio could completely denitrify the nitrate at inlet concentration up to 1250mg/l without nitrite accumulation. the maximum denitrification rate in the crbr was 3.56kgno3(-)/m(3)d with a nitrate reduction efficiency of 99% when the b ... | 2015 | 26369277 |
| assessment of biofilm formation of e. meningoseptica, d. acidovorans, and s. maltophilia in lens cases and their growth on recovery media. | bacterial biofilm formation in contact lens cases is a risk factor in the development of both microbial and infiltrative keratitis. this investigation evaluated three emerging pathogens: stenotrophomonas maltophilia, elizabethkingia meningoseptica, and delftia acidovorans for biofilm formation and metabolic activity in lens cases. also, growth of these bacteria on different media was assessed to optimize recovery conditions. | 2016 | 26383012 |
| update on infections caused by stenotrophomonas maltophilia with particular attention to resistance mechanisms and therapeutic options. | stenotrophomonas maltophilia is a gram-negative, biofilm-forming bacterium. although generally regarded as an organism of low virulence, s. maltophilia is an emerging multi-drug resistant opportunistic pathogen in hospital and community settings, especially among immunocompromised hosts. risk factors associated with s. maltophilia infection include underlying malignancy, cystic fibrosis, corticosteroid or immunosuppressant therapy, the presence of an indwelling central venous catheter and exposu ... | 2015 | 26388847 |
| iron is a signal for stenotrophomonas maltophilia biofilm formation, oxidative stress response, omps expression, and virulence. | stenotrophomonas maltophilia is an emerging nosocomial pathogen. in many bacteria iron availability regulates, through the fur system, not only iron homeostasis but also virulence. the aim of this work was to assess the role of iron on s. maltophilia biofilm formation, eps production, oxidative stress response, omps regulation, quorum sensing (qs), and virulence. studies were done on k279a and its isogenic fur mutant f60 cultured in the presence or absence of dipyridyl. this is the first report ... | 2015 | 26388863 |
| comparative genomics of non-pseudomonal bacterial species colonising paediatric cystic fibrosis patients. | the genetic disorder cystic fibrosis is a life-limiting condition affecting ∼70,000 people worldwide. targeted, early, treatment of the dominant infecting species, pseudomonas aeruginosa, has improved patient outcomes; however, there is concern that other species are now stepping in to take its place. in addition, the necessarily long-term antibiotic therapy received by these patients may be providing a suitable environment for the emergence of antibiotic resistance. to investigate these issues, ... | 2015 | 26401445 |
| fatty acid hydration activity of a recombinant escherichia coli-based biocatalyst is improved through targeting the oleate hydratase into the periplasm. | whole-cell biotransformation of fatty acids can be influenced by the activities of catalytic enzymes and by the efficiency of substrate transport into host cells. here, we improved fatty acid hydration activity of the recombinant escherichia coli expressing an oleate hydratase of stenotrophomonas maltophilia by targeting the catalytic enzyme into the periplasm instead of the cytoplasm. recombinant e. coli producing ohya in the periplasm under guidance of the pelb signal sequence (e. coli ohya_pp ... | 2015 | 26429801 |
| low prevalence of carbapenem-resistant bacteria in river water: resistance is mostly related to intrinsic mechanisms. | carbapenems are last-resort antibiotics to handle serious infections caused by multiresistant bacteria. the incidence of resistance to these antibiotics has been increasing and new resistance mechanisms have emerged. the dissemination of carbapenem resistance in the environment has been overlooked. the main goal of this research was to assess the prevalence and diversity of carbapenem-resistant bacteria in riverine ecosystems. the presence of frequently reported carbapenemase-encoding genes was ... | 2015 | 26430939 |
| cooperative pathogenicity in cystic fibrosis: stenotrophomonas maltophilia modulates pseudomonas aeruginosa virulence in mixed biofilm. | the present study was undertaken in order to understand more about the interaction occurring between s. maltophilia and p. aeruginosa, which are frequently co-isolated from cf airways. for this purpose, s. maltophilia rr7 and p. aeruginosa rr8 strains, co-isolated from the lung of a chronically infected cf patient during a pulmonary exacerbation episode, were evaluated for reciprocal effect during planktonic growth, adhesion and biofilm formation onto both polystyrene and cf bronchial cell monol ... | 2015 | 26441885 |
| effect of carbon on whole-biofilm metabolic response to high doses of streptomycin. | biofilms typically exist as complex communities comprising multiple species with the ability to adapt to a variety of harsh conditions. in clinical settings, antibiotic treatments based on planktonic susceptibility tests are often ineffective against biofilm infections. using a co2 evolution measurement system we delineated the real-time metabolic response in continuous flow biofilms to streptomycin doses much greater than their planktonic susceptibilities. stable biofilms from a multispecies cu ... | 2015 | 26441887 |
| stenotrophomonas maltophilia infections in adults: primary bacteremia and pneumonia. | stenotrophomonas maltophilia is the third most frequent non-fermentative gram-negative bacilli in nosocomial infections, and usually causes severe infections such as primary bacteremia and pneumonia. | 2015 | 26468367 |
| draft genome sequences of acinetobacter parvus cm11, acinetobacter radioresistens cm38, and stenotrophomonas maltophilia br12, isolated from murine proximal colonic tissue. | here, we report three genome sequences of bacteria isolated from murine proximal colonic tissue and identified as acinetobacter parvus cm11, acinetobacter radioresistens cm38, and stenotrophomonas maltophilia br12. | 2015 | 26472823 |
| genome sequence of a multidrug-resistant strain of stenotrophomonas maltophilia with carbapenem resistance, isolated from king abdullah medical city, makkah, saudi arabia. | the emergence and spread of multidrug-resistant (mdr) bacteria have been regarded as major challenges among health care-associated infections worldwide. here, we report the draft genome sequence of an mdr stenotrophomonas maltophilia strain isolated in 2014 from king abdulla medical city, makkah, saudi arabia. | 2015 | 26472828 |
| inoculation of paperboard mill sludge versus mixed culture bacteria for hydrogen production from paperboard mill wastewater. | a comparative evaluation of paperboard mill sludge (pms) versus mixed culture bacteria (mcb) as inoculum for hydrogen production from paperboard mill wastewater (pmw) was investigated. the experiments were conducted at different initial cultivation phs, inoculums to substrate ratios (isrs gvs/gcod), and hydraulic retention times (hrts). the peak hydrogen yield (hy) of 5.29 ± 0.16 and 1.22 ± 0.11 mmol/gcodinitial was occurred at ph = 5 for mcb and pms, respectively. at ph of 5, the hy and cod rem ... | 2016 | 26498965 |
| role of sul2 gene linked to transposase in resistance to trimethoprim/sulfamethoxazole among stenotrophomonas maltophilia isolates. | 2016 | 26522765 | |
| eps production and bioremoval of heavy metals by mixed and pure bacterial cultures isolated from ankara stream. | this study is focused on isolation of ni(ii), cu(ii) and cr(vi) resistant bacteria to assess their exopolysaccharide (eps) production and related bioremoval capacities. mixed cultures had higher heavy metal removal capacity in media with molasses (mas) than the control cultures lacking this carbon (as) containing 50 mg/l of heavy metal. the yields were 32%, 75.7%, and 51.1% in mas, while the corresponding values were 29%, 55.1%, and 34.5% in as, respectively. purification of the strains 1, 5 and ... | 2015 | 26524439 |
| clinical ineffectiveness of latamoxef for stenotrophomonas maltophilia infection. | stenotrophomonas maltophilia shows wide-spectrum resistance to antimicrobials and causes various infections in immunocompromised or critically ill patients with high mortality. in this era of antibiotics resistance, a revival of old antibiotics is now featured. we examined the clinical usefulness of latamoxef (lmox) for the treatment of s. maltophilia infection. | 2015 | 26527890 |
| bacteria associated with amblyomma cajennense tick eggs. | ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ... | 2015 | 26537602 |
| the inactivation of rnase g reduces the stenotrophomonas maltophilia susceptibility to quinolones by triggering the heat shock response. | quinolone resistance is usually due to mutations in the genes encoding bacterial topoisomerases. however, different reports have shown that neither clinical quinolone resistant isolates nor in vitro obtained stenotrophomonas maltophilia mutants present mutations in such genes. the mechanisms so far described consist on efflux pumps' overexpression. our objective is to get information on novel mechanisms of s. maltophilia quinolone resistance. for this purpose, a transposon-insertion mutant library ... | 2015 | 26539164 |
| antibiotic susceptibility of sulfamethoxazole-trimethoprim resistant stenotrophomonas maltophilia strains isolated at a tertiary care centre in hungary. | sulfamethoxazole-trimethoprim (sxt) is the drug-of-choice in stenotrophomonas maltophilia caused infections. there has been an increase in resistance to sxt of s. maltophilia over recent years. in this study 30 s. maltophilia clinical isolates resistant to sxt were investigated. antibiotic susceptibilities for ciprofloxacin, moxifloxacin, levofloxacin, doxycycline, tigecycline, ceftazidime, colistin and chloramphenicol were determined by broth microdilution method. none of the strains were susce ... | 2015 | 26551572 |
| prevalence of resistant gram-negative bacilli in bloodstream infection in febrile neutropenia patients undergoing hematopoietic stem cell transplantation: a single center retrospective cohort study. | bloodstream infection (bsi) is an important cause of morbidity and mortality in patients undergoing hematopoietic stem cell transplantation (hsct). to evaluate the causative bacteria and identify risk factors for bsi associated mortality in febrile neutropenia patients undergoing hsct, we collected the clinical and microbiological data from patients underwent hsct between 2008 and 2014 and performed a retrospective analysis. throughout the study period, among 348 episodes of neutropenic fever in ... | 2015 | 26559260 |
| antimicrobial susceptibility pattern of burkholderia cepacia complex & stenotrophomonas maltophilia over six years (2007-2012). | 2015 | 26609043 | |
| laboratory diagnosis, clinical management and infection control of the infections caused by extensively drug-resistant gram-negative bacilli: a chinese consensus statement. | extensively drug-resistant (xdr) gram-negative bacilli (gnb) are defined as bacterial isolates susceptible to two or fewer antimicrobial categories. xdr-gnb mainly occur in enterobacteriaceae, acinetobacter baumannii, pseudomonas aeruginosa, and stenotrophomonas maltophilia. the prevalence of xdr-gnb is on the rise in china and in other countries, and it poses a major public health threat as a result of the lack of adequate therapeutic options. a group of chinese clinical experts, microbiologist ... | 2016 | 26627340 |