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b-cell activating properties of polymyxin b.polymyxins are known to be inhibitors of certain polyclonal b cell activators such as lipopolysaccharide and dextransulphate. however, increased specific responses to hapten-coupled mitogens have been reported after the addition of polymyxins to superoptimal conjugate doses. in this paper we have studied the effect of polymyxin b on superoptimal polyclonal doses of lipopolysaccharide. results similar to those reported for hapten-lipopolysaccharide conjugates were obtained. polymyxin b was also f ...1978204983
burro aortic collagen: platelet aggregating activity and ultrastructural changes induced by plasma.a fibrillar collagen molecule was extracted from the upper thoracic aorta of an old burro (equus asinus). presence of the collagen in the extract was determined by amino acid analysis, scanning and transmission electron microscopy, incubation with collagenase, and assays of its platelet-aggregating capacity by "aggregometry". based on the amino acid rations of proline/hydroxyproline and lysine/hydroxylysine, the collagenous protein most nearly resembles type i of 4 main published types of collag ...1978205146
endocrine mechanisms of parturition. 1978205168
changes in the sarcolemma of the hypothyroid heart. 1978205215
prostaglandin biosynthesis and catabolism in the lamb ductus arteriosus, aorta and pulmonary artery.homogenates of tissues from fetal and neonatal lamb ductus arteriosus, aorta and pulmonary artery have the capacity to convert arachidonic acid as well as the intermediate prostaglandin endoperoxide, prostaglandin h2, into three products: prostaglandins e2, f2alpha and a major product 6-ketoprostaglandin f1alpha. the three tissues also displayed prostaglandin 15-hydroxydehydrogenase and 13-reductase catabolic activities. the catabolishing system showed considerable substrate specificity: prostag ...1978205256
characterization of a unique cell line (laz 221) from human acute lymphocytic ("null" cell) leukemia.a unique human cell line designated laz 221 has been established from the peripheral blood of a patient with acute lymphocytic leukemia of the "null" cell type. the cell line does not possess the epstein-barr virus nuclear antigen and has a karyotype of 45,xx,-9,-12,+(9q12q). both the established cell line and the patient's uncultured blast cells share the same phenotypic markers. they both lack t-cell markers. they fail to form sheep erythrocyte rosettes and do not react with t-cell-specific an ...1978205365
the fetal hypothalamus and pituitary in the initiation of labour.evidence is presented for a number of events in the fetal hypothalamic-pituitary axis which may play a key role in the onset of labour: (1) in the sheep fetus a progressive rise in the fetal circulating concentrations of corticotropin in the days preceding delivery; (2) in the human fetus a switch from the production of corticotropin-like fragments (melanotropin and corticotropin-like intermediate lobe peptide) to authentic corticotropin in the last weeks of gestation; there is evidence also for ...1977205396
parturition in the sheep.parturition in sheep is initiated by a sharp rise in the rate of secretion of cortisol by the fetal adrenal. increased secretion is due partly to enhanced responsiveness to corticotropin (acth) and partly to increased fetal concentrations of corticotropin. cortisol acts on placental enzymes active in the biosynthesis of oestrogens from progesterone. thus placental secretion of oestrogen increases and that of progesterone decreases. this change in the ratio of oestrogen: progesterone, particularl ...1977205398
the role and regulation of corticotropin in the fetal sheep. 1977205399
pharmacological control of the hormonally modulated immune response. ii. blockade of antibody production by a combination of drugs acting on neuroendocrine functions. its prevention by gonadotropins and corticotrophin.injection of a combination of three drugs, 5-hydroxytryptophan, the alpha-blocker phentolamine and the neuroleptic drug haloperidol into mice before or together with sheep red blood cells (srbc) induces a complete and long-lasting inhibition of antibody production to srbc and leads to specific unresponsiveness. the mice unresponsive to srbc respond normally to another antigen. treatment with a combination of luteotropic (lh), follicle stimulating (fsh) and corticotropic hormone (acth) before adm ...1978205502
isolation of paravaccinia virus from sheep. 1978205699
role of acth, angiotensin and potassium in stress-induced aldosterone secretion. 1978205729
the role of catecholamines in lung liquid absorption at birth.we have examined the effect on lung liquid secretion of catecholamines infused in chronically catheterized fetal lambs in utero. isoproterenol and epinephrine inhibited secretion, an effect which increased with gestation and, in fetuses near delivery, caused absorption of lung liquid. in 7 out of 8 experiments nor-epinephrine had no effect on secretion. this pattern of response and the fact that the inhibitory effect could be blocked by propranolol indicate a mode of action involving beta-adrene ...1978205826
immunosuppression in chickens by passive transfer of preparations of specific immunoglobulins.the immunosuppressive effects of passive transfers of specific immunoglobulin preparations obtained from immune or hyperimmune sera were evaluated in chickens which were challenged with sheep red blood cells. the data indicate that igy, fractionated from immune sera, and igy and igm, fractionated from hyperimmune sera, when passively administered at the doses employed in this study resulted in antibody-mediated suppression.1977205849
subversion of the immune system by tumors as a mechanism of their escape from immune rejection. 1978205882
chemotactic antibody.antibody of the immunoglobulin g class to herpes simplex virus and antibody of the immunoglobulin m class to sheep red blood cells were coupled to the synthetic peptide formylmethionylleucylphenylalanine (fmet-leu-phe), which is chemotactic for both mononuclear and polymorphonuclear leukocytes. the resulting molecules were chemotactic and retained their antigen-binding activity. when antibodies coupled to fmet-leu-phe were incubated with antigen, the resulting immune complexes were also chemotac ...1978205950
cultivation of sheep pox virus on the chorioallantoic membrane of chicken embryo. 1978205986
the immunoprophylaxis of of rotavirus infections in lambs.the effect of colostrum or serum feeding on subsequent rotavirus infections was investigated in neonatal gnotobiotic lambs. immunity after feeding colostrum did not depend on absorption of passively acquired antibody into the circulation. protection against clinical disease depended on the volume of colostrum ingested. the protection afforded by feeding serum was specific, since serum free of rotavirus antibody failed to confer protection. immune serum fed at a rate of 2.5 ml per kg twice daily ...1978205988
[isolation of the parainfluenza-3 virus from lambs]. 1978205991
[immunogenicity of an inactivated culture vaccine against aujeszky's disease]. 1978205992
erythropoietin and the control of red cell production. 1978206191
species of differences in postganglionic motor transmission to the retractor penis muscle.1 graded motor responses were elicited in isolated, desheathed, thin strips of dog, horse, pig and sheep retractor penis (rp) muscles by field stimulation with trains of 0.2 ms pulses at 10 hertz. these twitches were shown to be neurogenic in all four species, by their prompt extinction in tetrodotoxin.2 alpha-adrenoceptor blocking drugs abolished the contractile response to noradrenaline and to tyramine in all four species.3 motor transmission was wholly adrenergic in the horse as in the dog rp ...1978206306
[effect of periodate oxidation of glycoconjugates of lymphocyte membranes on the immune response in vitro].mouse spleen cells treated with sodium periodate for 10 min. at 4 degrees c are stimulated to undergo blastogenesis and to incorporate thymidine. the effect of such treatment on the antibody response in vitro induced by sheep red blood cells has been evaluated. periodate-induced proliferation is accompanied by a marked inhibition of the immune response to this antigen. at concentrations leading to mitogenesis, no cytotoxic effect of periodate was observed and treated cells survived well on tissu ...1978206378
study of the in vitro pharmacological effects of gram-negative endotoxins in parallel with toxicity testing in normal adult guinea-pigs. 1978206397
equilibrium and kinetic studies of unfolding of homologous cytochromes c.extensive investigations of the unfolding equilibria and kinetics of oxidized and reduced cytochromes c are reported. it is found that all cytochromes c have similar unfolding free energies (deltagd = 7 +/- 1 kcal/mol). differences among species do not correlate in any way with the metabolic differences among species. the stabilization of cytochrome c on reduction is estimated at 1.1 kcal/mol. stability differences among species are mirrored in their denaturation kinetics. for cytochrome c (iii) ...1978206558
bovine and ovine leukemia viruses. i. characterization of viral antigens.a comparative study on several virus-specific antigens of bovine and ovine leukemia viruses is presented. in addition to the major immunologically reactive, nonglycosylated antigen p21, which has a molecular weight of 21,000, both viruses share two glycoproteins of apparent molecular weights of 60,000 (gp60) and 32,000 (gp32), respectively. immunological cross-reaction suggests that ovine and bovine leukemia viruses are genetically highly related.1978206723
the immunological response to intact and dissociated blue-tongue virus in mice.antigenic fractions of bluetongue virus were separated by ultracentrifugation in tris-buffered cscl gradients at ph 6, 7 or 8 and the bluetongue virus polypeptide composition of the bands isolated from these gradeints was monitored by polyacrylamide gel slab electrophoresis. the immunological response to these fractions in mice was determined by a haemolytic plaque-forming cell assay, using sheep erythrocytes onto which intact bluetongue virus was adsorbed as lytic indicator cells. isolated oute ...1977206861
detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus.an antigen immunologically related to a group-specific antigen (gp52, a 52,000-dalton glycoprotein) of the mouse mammary tumor virus has been identified in paraffin sections of human breast cancers by means of the indirect immunoperoxidase technique. the specificity of the reaction with antibody against mouse mammary tumor virus was examined by absorption of the igg with the following: (a) purified gp52; (b) a number of virus preparations (mouse mammary tumor virus, rauscher leukemia virus, simi ...1978206905
changes in leucocyte cytochrome oxidase activity associated with deficiency of copper in laboratory and farm animals.the cytochrome oxidase activity of circulating leucocytes was investigated by means of a semiquantitative method using blood films. a significant decrease in activity was found in male rats, cattle and sheep that had been deprived of copper. during copper depletion, leucocyte cytochrome oxidase activity declined more slowly than did plasma copper concentration and plasma ferroxidase i activity, and so was less sensitive as a guide to the copper status of the animal.1978206932
experimental infection of calves with an adenovirus isolated from sheep and related to bovine adenovirus type 2. ii. pathological and histopathological studies. 1978207053
challenge experiments with the virus of aujeszky's disease in non-vaccinated and vaccinated sheep. 1978207055
transmission of a mucosal disease virus infection between sheep.the transmission of mucosal disease virus (mdv) from infected ewes and their lambs to susceptible sheep was investigated. mdv was recovered from the amniotic fluid of an infected pregnant ewe and from the blood, nasal swabs and urine of hairy lambs. mdv infection was transmitted either at lambing, from infective foetal fluids or lambs, or later as a result of contact with a surviving with a hairy lamb and either aborted or gave birth to an infected hairy lamb. adult sheep and 12-months-old sheep ...1977207251
bluetongue in cattle: a review. 1978207405
the antibody response in sheep infected with a kenyan sheep and goat pox virus. 1978207747
experimental infection of specific pathogen-free lambs with parainfluenza virus type 3 and pasteurella haemolytica. 1978207749
the effect of vaccination with a parainfluenza type 3 virus on pneumonia resulting from infection with parainfluenza type 3 virus and pasteurella haemolytica. 1978207750
immunosuppressive activity of antibody directed against endogenous c-type virus interferes with early events of the immune response.we have analyzed the effects of an antiserum prepared against balb/c endogenous xenotropic c-type virus on the humoral immune response of mice. both in vivo and in vitro, this serum suppresses the response to sheep red blood cells, an effect that can be absorbed out by purified balb/c xenotropic c-type virus or friend leukemia virus, but not by rous sarcoma virus. the serum produces its maximum effect when administered together with or before the antigen, but not 24 hr later. this suggests that ...1978207777
complement-induced modifications in membrane fluidity: studies with resealed and glutaraldehyde-treated erythrocyte membrane ghosts. 1978207778
modulation of the antibody response to sheep erythrocytes in murine spleen cell cultures by a t cell mitogen extracted from bordetella pertussis. 1978207953
concurrent infection of lambs with parainfluenza virus type 3 and pasteurella haemolytica. 1977208027
sheep erythrocyte and bluetongue virus antibody responses of spleen cell cultures from mice.the optimum conditions for the culture of cells from dissociated spleens were determined. routinely, 10(7) cells were seeded per ml of rpmi 1640 medium supplemented with 20% pre-tested foetal calf serum. for the assay of the immune response, cultures were supplemented with 30 mumolar mercaptoethanol. the immune responses to sheep erythrocyte and bluetongue virus antigens were determined by the haemolytic plaque-forming cell assays described by oellermann (1974) and oellermann, carter & marx (197 ...1977208037
cell fusion induced by scrapie and creutzfeldt-jakob virus-infected brain preparations.cell fusion was induced by brain extracts containing the scrapie virus and the virus of creutzfeldt-jakob disease. the assay involved quantitation of colony-forming ability in a double selection system, strandardized against fusion induced by sendai virus. correlation between the logarithm of virus dilution and the hybrid colony number gave similar curves for scrapie virus and sendai virus. fusion induction may explain some aspects of pathogenesis in these diseases and provide a potential in vit ...1978208080
serological evidence of the occurrence of bluetongue in iraq.precipitating antibodies against bluetongue were detected in sheep and goat serum samples collected from animals slaughtered in baghdad abattoir. out of 294 sheep serum samples and 110 goat serum samples examined, 28 and 18 samples respectively showed precipitating activity. in addition, examination of sheep serum samples collected from localities where clinical cases similar to bluetongue were previously reported revealed the presence of bluetongue precipitating antibodies in 101 sera out of 19 ...1978208202
[immunofluorescence with the pi-3 virus--study of various working conditions for the detection of viral infection using immunofluorescence on various types of cell cultures].we used the fluorescence method for the investigation of the sensitivity of several kinds of cell cultures to the infection with the parainfluenza virus 3 (pi-3). cultures from calf kidneys were the most sensitive while we did not determine any differences between primary cultures and cultures of the first and second subpassages and/or freshly cultivated or incubated cultures over seven days at a temperature of 37 degrees c. equal values of infection titres like on the cultures of calf kidneys w ...1978208220
isolation of louping-ill virus from red deer (cervus elaphus). 1978208224
[rapid diagnosis of infections caused by the chickenpox-herpes zoster viruses].a diagnostic preparation for the indirect hemagglutination test (iha) was obtained by sensitization of fixed tannin-treated sheep erythrocytes with igg-fractions from human herpes zoster convalescent sera. a high specificity of the preparation was established in comparative experiments. it could detect the varicella-herpes-zoster virus antigen in 100% of specimens from patients. the iha test with this erythrocyte diagnostic preparation is simple, economic, gives the results within 1-1 1/2 hours ...1978208309
aldosterone, corticosterone, cortisol, 11-deoxycortisol and 11-deoxycorticosterone concentrations in the blood of chronically cannulated ovine foetuses: effect of acth. 1978208342
regulation of aldosterone secretion: current concepts and newer aspects.four humoral factors have been shown to play important roles in the regulation of aldosterone secretion. these are acth, potassium, sodium and angiotensin ii. acth appears to play little or no role in the maintenance of adrenal zona glomerulosa cells in response to specific stimuli. however, there is evidence to show that physiologic levels of acth regulate the minute-to-minute fluctuations of plasma aldosterone. in man and in experimental animals, alterations in potassium balance as well as ac ...1977208405
steady-state and pre-steady kinetic studies on mitochondrial sheep liver aldehyde dehydrogenase. a comparison with the cytoplasmic enzyme.kinetic studies were carried out on mitochondrial aldehyde dehydrogenase (ec 1.2.1.3) isolated from sheep liver. steady-state studies over a wide range of acetaldehyde concentrations gave a non-linear double-reciprocal plot. the dissociation of nadh from the enzyme was a biphasic process with decay constants 0.6s-1 and 0.09s-1. pre-steady-state kinetic data with propionaldehyde as substrate could be fitted by using the same burst rate constant (12 +/- 3s-1) over a wide range of propionaldehyde c ...1978208506
kinetic studies on the esterase activity of cytoplasmic sheep liver aldehyde dehydrogenase.the hydrolysis of 4-nitrophenyl acetate catalysed by cytoplasmic aldehyde dehydrogenase (ec 1.2.1.3) from sheep liver was studied by steady-state and transient kinetic techniques. nad+ and nadh stimulated the steady-state rate of ester hydrolysis at concentrations expected on the basis of their michaelis constants from the dehydrogenase reaction. at higher concentrations of the coenzymes, both nad+ and nadh inhibited the reaction competitively with respect to 4-nitrophenyl acetate, with inhibiti ...1978208507
comparison of firing patterns and sensory responsiveness between supraoptic and other hypothalamic neurons in the unanesthetized sheep.adult southdown ewes were surgically prepared with pituitary stimulating electrodes, carotid and jugular cannulae, and a cranial platform-cylinder arrangement for chronic single unit recording. isolated neurons (n = 112) in the region of the supraoptic nucleus (son) were identified by pituitary stalk stimulastion as ad + (antidromically invaded) son neuroendocrine cells (n = 75) or ad--(not antidromically invaded) son neurons (n = 37). spontaneous firing pattern distribution and sensory evoked b ...1978208711
the interaction of human plasma glycosaminoglycans with plasma lipoproteins. ii. hemagglutination studies.formalinized, tannic acid-treated sheep erythrocytes coated with low density lipoproteins (lsl) or apoprotein b (apo-b) are are agglutinated by anti-apo-b immunserum. those coated with high density lipoproteins (hdl) or apoprotein a-i(apo-a-i) are agglutinated by anti-apo-a-i immunserum. these coated formocells have been used to study the interactions of lipoproteins and apoproteins with plasma glycosaminoglycans (gag). the sulfate-rich species of plasma gag agglutinates cells coated with ldl, h ...1978208802
bone marrow-derived lymphoid cell lines from patients with agammaglobulinemia.in vitro infection with epstein-barr virus of bone marrow-derived (b) lymphocytes from blood of patients with x-linked or common varied agammaglobulinemia resulted in the establishment of long-term b-lymphoid cell lines (lcl). lcl were established only with b lymphocytes from patients whose b cells failed to respond to in vitro mitogenic stimulation. in contrast, lymphocytes from patients without small b lymphocytes or with b cells that synthesized but failed to secrete ig failed uniformly to es ...1978209061
antibody responses to vaccination against louping-ill virus in newborn lambs. 1978209066
[role of prolactin in the development and initiation of milk secretion]. 1978209078
detection of proviral dna in horse cells infected with equine infectious anemia virus.equine infectious anemia virus (eiav) recently has been shown to possess a high-molecular-weight rna genome and a virion reverse transcriptase. we completed the demonstration that eiav is a retrovirus by showing the presence of proviral dna in equine cells infected in vitro, but not in normal horse dna. these studies were performed by using a highly representative cdna probe synthesized by the virion polymerase. it was found that this cdna reassociated extensively, and with high thermal stabilit ...1978209211
the isolation of lamellar bodies and their membranous content from rat lung, lamb tracheal fluid and human amniotic fluid. 1978209279
ovine pneumonia. 1977209377
alpha and angiotensin receptor tone in the near-term sheep fetus. 1978209472
direct photo-affinity labeling with cyclic nucleotides: action spectra. 1978209486
investigations on the presence of antibodies to alphaviruses, flaviviruses, bunyavirus and kemerovo virus in humans and some domestic animals.the presence of antibodies to some alphaviruses, flaviviruses, bunyaviruses and orbiviruses was studied in the sera from humans and domestic animals of biotopes "c" and "t" in the south-east of romania. specific antibodies were found in humans to 3 alphaviruses (eastern equine encephalitis, western equine encephalitis and sindbis), in a very low proportion, and to 3 flaviviruses (tickborne encephalitis, west nile and ntaya), at a much higher percentage. very low percentages of antibodies to buny ...1978209613
[orf and milkers nodules]. 1978209716
homeostasis of the fetal circulation and the part played by hormones. 1977209720
environmental considerations for the disposal of pbb-contaminated animals and wastes.accidental contamination of livestock feed in 1973 by polybrominated biphenyls (pbb) led to the destruction of over 30,000 animals in michigan. animal carcasses of mostly dairy cattle along with some beef cattle, hogs, sheep and rabbits destroyed under the federal food and drug administration guidelines were disposed on the land at an environmentally safe site in kalkaska county, michigan. the geology and hydrology of the disposal site on state-owned land is considered favorable for the disposal ...1978209986
influence of the sialic acid content of membranes on their sensitivity to silica and aluminate-modified silica. 1978210014
effect of the association time of in vivo bound prolactin on the [125i]prolactin receptor assays of female rat livers.significantly (p less than 0.01) reduced 125i-labeled ovine prolactin binding (mean, --22%), as a result of an ether anesthesia-reduced rise in serum prolactin, was observed in plasma membrane preparations of liver samples of female rats taken after 5 min of etherization when compared to samples taken from the same animals during the first minute of etherization. this reduction in assayable receptors occurred after 1 h but not 2 h of assay incubation time. significantly (p less than 0.05) reduce ...1978210063
in-vitro effects of clostridium welchii type-d epsilon toxin on guinea-pig, mouse, rabbit and sheep cells.epsilon toxin, at relatively low concentrations, killed guinea-pig peritoneal macrophages in vitro. the cells became swollen, the nuclear and cytoplasmic membranes "blistered" and discontinuous, and the cytoplasm appeared structureless. formalinised epsilon prototoxin was shown to bind closely to the outer surface of the cells and it is concluded that this site represents the location of the receptors for epsilon toxin. in addition the toxin at higher concentrations killed rabbit peritoneal macr ...1978210279
ovine pneumonia. 1978210432
distribution of crf activity and immunoreactive acth within the hypothalamic-neurophypophyseal complex in various species. 1978210465
action of pmsg and asialo-pmsg on rat leydig and granulosa cells.highly purified pregnant mares serum gonadotropin (pmsg) was nearly as potent as ovine luteinizing hormone (lh) and human follicle stimulating hormone (hfsh) when bioassayed in vitro in systems known to respond primarily to lh or fsh. an analogue of human chorionic gonadotropin treated with neuraminidase, galactosidase, beta-n-acetylglucosaminidase, and mannosidase (hcg) inhibited the stimulatory effects of hcg, lh, and pmgs on camp accumulation in rat leydig cells but did not inhibit the stimul ...1978210675
conjugated and unconjugated estrogens in fetal and maternal fluids of the pregnant ewe: a possible role for estrone sulfate during early pregnancy. 1978210852
[bioligical aspects of cell fusion induced in vitro by sheep visna virus].visna virus induced cell fusion of sheep choroid plexus cells was explored in vitro. fusion is early rapid, and of exogenous origin for multiplicities of infection equal to or greater than 2 ufp per cell; whereas fusion is slow, late-occurring and of endogenous origin for multiplicities of infection less than or equal to 0.75 ufp per cell.1978210900
[kinetics and ultrastructure of sheep fibroblast fusion induced by polyethylene glycol. comparison with endogenous cell fusion induced by visna virus].the authors compare the fusion of sheep fibroblasts induced by low multiplicities of infection using visna virus and by high concentrations of polyethylene-glycol. in the case of visna virus cell fusion is of the endogenous type, while fusion induced by polyethylene-glycol is of the exogenous type. the ultrastructural features are discussed for each type of cell fusion. the main differences between the two systems involve the intracellular microfilaments and golgi apparatus.1978210901
enterotoxigenic clostridium perfringens type a isolated from intestinal contents of cattle, sheep and chickens.one hundred and fourteen strains of clostridium perfringens, isolated from the intestinal contents of cattle, sheep, and chickens with enteritis or other disease conditions were studied for their ability to produce enterotoxin. reversed passive hemagglutination, fluorescent antibody and immunodiffusion tests were used. on the basis of the reversed passive hemagglutination titres, supported by the other two tests, enterotoxigenicity of the strains was arbitrarily classified into two categories: h ...1978210914
the demonstration of cells bearing fc receptors in the metrial gland of the pregnant rat uterus.cells obtained by collagenase treatment of metrial gland tissue from rats of day 12, 13, 14 and 15 of pregnancy were examined for the presence of surface membrane receptors for immunoglobulin (fc receptors). using an ea rosetting technique in which sheep red blood cells (srbc) were sensitized with a rabbit anti-srbc immunoglobulin preparation, fc receptors were found on a proportion of the cells. the majority of the granulated metrial gland cells were not included in the rosetting cell populatio ...1978210953
interaction of rumen development and renal gluconeogenesis in lambs.renal gluconeogenesis of equal aged lambs with different states of rumen development was studied. going from preruminantto ruminant stage, the rate of glucose formation from several precursors increased. concomitantly urine ph shifted from acid to base state. obviously, an enhanced secretion of h+ in lambs kidney during rumen development does not stimulate renal gluconeogenesis. furthermore, there is no indication from our data that renal gluconeogenesis in lambs is inversely related to the acti ...1977210999
infection of taiwan monkey t and b cells with epstein-barr virus.in order to establish taiwan monkey lymphoblastoid cell lines, attempts were made to raise the susceptibility of monkey lymphocytes to epstein-barr virus (ebv) by chemical and enzymatic treatments. ebv infectivity to monkey t and b cells were tested by detection of ebv early antigens in infected cells with the indirect immunofluorescent antibody technique. treatments of monkey unfractionated lymphocytes with deae-dextran (160 microgram/ml) for 1 hr, ethylenediamine tetra-acetic acid (edta, 0.5%) ...1977211014
[histologic and serologic studies for the detection of maedi virus infections in a herd of sheep]. 1978211022
the effects of alpha and beta adrenergic agents on spleen cell antigen binding in four amphibian species.adults of two urodele amphibian species (triturus cristatus carnifex and cynops hongkongensis) and two anuran species (rana temporaria and xenopus laevis laevis) were immunized with a 25% suspension of sheep or horse erythrocytes. after eight or 14 days, splenic lymphocytes were removed, and their specific red cell-binding capacities tested by immunocytoadherence. antigen-binding cells were classified as high-dose nonsecretory (s-) or secretory (s+), according to whether they bound a single laye ...1978211039
initiation of sperm motility in the mammalian epididymis.spermatozoa acquire the capacity for motility as they traverse the mammalian epididymis. the biochemical basis for this induction of motility is still largely unknown. current theories are discussed and recent studies from the authors' laboratory are described which indicate that two separate processes are involved and that these act synergistically. these processes are an increase in the intrasperm content of cyclic amp during epididymal transit combined with the binding of a specific forward-m ...1978211050
hydrolytic action of phospholipases on sealed ghosts of mammalian erythrocytes. 1978211122
ultrastructural changes in the placenta of the ewe after fetal pituitary stalk section.binucleate cells are a normal component of the ovine chorionic epithelium, but are usually separated from the fetal-maternal interface by a thin layer of cytoplasm derived from the principal or uni-nucleate cells of the trophoblast. they are distinguished not only by two distinct and separate nuclei, but also by conspicuous membrane-bound cytoplasmic inclusions in the form of haloed droplets. after fetal pituitary stalk section binucleate cells move up to and participate in the formation of the ...1978211539
antibody titres to lamb rotavirus in colostrum and milk of vaccinated ewes.ewes were vaccinated two to three weeks prior to mating with a formalin-treated preparation of lamb rotavirus. the colostrum and milk produced by vaccinated ewes after the subsequent pregnancy were shown to contain significantly higher titres of antibody to the virus than did mammary secretions from non-vaccinates. the virus neutralising antibody activity was associated with igg in both colostrum and milk. however, igg concentrations in the mammary secretions of vaccinates and non-vaccinates did ...1978211697
[radial immunodiffusion reaction in research with the foot-and-mouth disease virus]. 1978211706
effects of human chorionic gonadotrophin on the release of cyclic amp and progesterone from the sheep ovary at different stages of the oestrous cycle.the rate of release of cyclic amp by sheep ovaries containing a corpus luteum was determined at different stages of the cycle before and up to 60 min after an intra-arterial (ia) injection of 500 iu human chorionic gonadotrophin (hcg). the median cyclic amp concentration in arterial plasma and of ovarian venous plasma following hcg stimulation was 93.2 and 98.0 pmol/ml, respectively. the ovaries of ewes examined at days 1 and 2 of the cycle showed no response to hcg, whereas in 2 sheep at day 3, ...1978211774
maturational changes in sheep ovarian follicles: gonadotrophic stimulation of cyclic amp production by isolated theca and granulosa cells.theca and granulosa tissues isolated from sheep ovarian follicles of different sizes were incubated in the presence of human chorionic gonadotrophin (hcg; 5 iu/ml) or follicle stimulating hormone (fsh; 5 microgram nih-fsh-s11/ml) for 40 min. changes in the total amounts of cyclic 3',5'-adenosine monophosphate (camp) were used as an index of the responsiveness of these preparations to the hormones. thecal tissue of both large (4-6 mm in diameter) and small (1-3 mm) follicles responded similarly t ...1978211775
virus infection in demyelinating diseases.several animal and human demyelinating diseases of the central nervous system (cns) are associated with rna or dna viruses. these viruses infect cns cells lytically or persistently. they mainly belong to the group of envelope viruses which derive their envelope partly from the host cell membrane. the process of virus release may result in the appearance of new antigens of virus-infected cells or the incorporation of cell membrane material into the viral envelope. these changes may lead to an imm ...1978211825
[assay of human anti-exoproteins to group a streptococci by microhaemagglutination: correlation with anti-streptolysin o and various anti-enzymes (author's transl)].a microhaemagglutination test in disposable u plates has been devised for rapid, quantitative evaluation in antistreptococcal antibodies in human sera. fresh or freeze-dried glutaraldehyde-treated sheep erythrocytes sensitized with over fifteen extracellular proteins released by group a streptococcus pyogenes including streptolysin o, deoxyribonucleases, hyaluronatelyase, streptokinase and nicotinamide dinucleotide glycohydrolase were used. haemagglutination and anti-streptolysin o (aslo) titers ...1978211892
clonal growth of entamoeba in agar: some applications of this technique to the study of their cell biology.a new technique for growing single entamoeba trophozoites into colonies in agar has been developed. this method depended upon axenic cultivation and utilized diamond's new tyi-s-33 medium. the present paper describes several different types of experiment which have utilized the agar technique. a) cloning. the isolation of clones from agar way easy and successful. clones of the hm-1 strain did not differ in virulence for newborn hamster liver or in colony morphology or colony forming efficiency. ...1978211957
[serology with polysaccharides of entamoeba histolytica].the immunochemical activity of a polysaccharidic antigen derivated from e. histolytica was studied. this polysaccharide was tested by means of counterimmunoelectrophoresis (cie) and radial immunodiffusion (idr) against sera from patients with diagnosis of intestinal and hepatic amebiasis. in order to perform a test most significative to the problem sera, these were submitted previously to passive hemagglutination, cie and idr with total ameba antigen. in hepatic amebiasis it can be observed that ...1978211962
mitogen-induced activation of stable e-rosettes by human lymphocytes.following on an earlier study which demonstrated that pha can activate human lymphocytes to form temperature-stable (37 degrees c.) e-rosettes with sheep red blood cells, a number of other mitogens were tested. these included concanavalin a, pokeweed mitogen, soybean agglutinin, sodium periodate, and galactose oxidase. all except soybean agglutinin demonstrated the capability to activate lymphocytes to form stable e-rosettes.1978212361
differences in the capacity of simian virus 40 (sv40) tumor antigens on cells, membranes and in soluble form to induce transplantation immunity in hamsters and mice.the immunogenicity of the sv40 tumor-specific transplantation antigen (tsta) on cells, cell particulates and solubilized membranes was studied in mice and in syrian hamsters. immunizations were done with various concentrations of tissue-culture-passaged, non-virus-releasing transformed cells, purified cell membranes and in some cases purified nuclei and papain-solubilized membranes obtained from several species, including the mouse, hamster, man, and sheep. all transformed cell lines were t-anti ...1978212373
detection of differences among human and animal rotaviruses, using analysis of viral rna. 1978212394
passive immunity in rotaviral infections. 1978212400
comments on passive immunity in rotaviral infections. 1978212401
rickettsial indirect hemagglutination test: isolation of erythrocyte-sensitizing substance.the erythrocyte-sensitizing substances (ess) of rickettsia prowazekii and r. conorii were characterized by biological and chemical criteria. ess could be derived from either soluble or particulate complement-fixing antigens obtained by ether extraction of rickettsiae. the soluble complement-fixing antigen exhibited two peaks of serological activity in potassium tartrate density gradients. the particulate complement-fixing antigen coincided with the more dense peak but was distinguishable by its ...1978212448
synergistic lysis of erythrocytes by propionibacterium acnes.sheep and human erythrocytes, partially processed by staphylococcus aureus or clostridium perfringens, were susceptible to lysis in the presence of propionibacterium acnes. p. acnes liberated a lipase that was detected on tween 80 agar and also on phospholipase c-precipitated egg yolk agar. such a lipase might have contributed in the process of an intensified cellular lysis. similar reactions were attempted with lactobacillus acidophilus, known to possess a nondiffusible lipase, and failed to pr ...1978212450
possible windborne spread of bluetongue to portugal, june-july 1956.the possible sources for the epidemic of bluetongue in portugal at the beginning of july 1956 were examined. introduction through authorized importation of domestic or wild ruminants was not feasible, since no cattle, sheep or goats were imported and the wild ruminants were confined to lisbon zoo, which was too far from the initial outbreaks. weather maps were examined to see if the wind could have carried infected culicoides midges from north africa. on 21 june 1956 infected midges in morocco c ...1978212475
studies of louping-ill virus (flavivirus group) in wild red grouse (lagopus lagopus scoticus).studies were made to find evidence of louping-ill virus infection in free-living red grouse and relate this to their breeding success. in areas where ticks were abundant 61 (84%) adult grouse had antibody to the virus compared with 1 (10%) in areas where ticks were relatively scarce. of 162 chicks tested 25 were shown to be viraemic. infected chicks were of significantly less weight than comparably aged uninfected birds and the probability that they died was much greater than that of uninfected ...1978212479
early immune responses in visna, a slow viral disease of sheep. 1978212487
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