Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| recombinant carp (cyprinus carpio) growth hormone: expression, purification, and determination of biological activity in vitro and in vivo. | carp growth hormone (cgh) cdna (koren et al., 1989) was cloned under the control of lambda-phage plol promoter and lambda cll ribosomal binding site into pbr322 plasmid to enable its expression in escherichia coli a1645 that produces constitutively the thermolabile lambda repressor c1857. temperature shift to 42 degrees abolished the repression, resulting in a high level of cgh expression. the bacterially expressed cgh protein, contained within the refractile body pellet, was solubilized in 4.5 ... | 1993 | 8428649 |
| cloning and sequence analysis of cdnas encoding the mhc class ii beta chain in atlantic salmon (salmo salar). | atlantic salmon (salmo salar) cdnas encoding the major histocompatibility complex (mhc-sasa) class ii beta chain were isolated from a leucocyte library by a polymerase chain reaction (pcr) approach. three different cdnas (c144, c22, and c157) encoding the entire mature beta chain have been analyzed. clone c144 differs from clone c157 in 12.6% of the nucleotides in the beta 1-encoding region. the corresponding differences between clones c144 and c22, and clones c22 and c157, are 10.3% and 5.2%, r ... | 1993 | 8436418 |
| spinule-type neurite outgrowth from horizontal cells during light adaptation in the carp retina: an actin-dependent process. | dendrites of horizontal cells in the carp retina which invaginate the cone pedicles form numerous spinules during light adaptation. we have analyzed the contribution of cytoskeletal elements to this process. isolated horizontal cells and frozen sections were screened with phalloidin for the existence of f-actin. f-actin was present in all types of horizontal cells and particularly enriched in the distal parts of the dendrites. electron microscopical analysis demonstrated that interruption of the ... | 1993 | 8445409 |
| brain sensitivity to anoxia in fish as reflected by changes in extracellular k+ activity. | most vertebrates have a very limited tolerance to anoxia. the only exceptions to this rule are found among ectothermic species, notably crucian carp (carassius carassius) and freshwater turtles. it has been assumed that the brains of these species are less sensitive to anoxia than ectothermic brains in general. however, it has not been possible to exclude that anoxia-intolerant species like rainbow trout (oncorhynchus mykiss) die from heart failure rather than brain failure during anoxia, and th ... | 1993 | 8447481 |
| the gut-associated lymphoid tissue (galt) of carp (cyprinus carpio l.): an immunocytochemical analysis. | the galt of carp was studied with monoclonal antibodies reacting with carp ig or carp leukocytes, using (dual) immunofluorescence or immunogold staining on cryosections, cytocentrifuge slides, and cell suspensions of the intestine. the intestinal epithelium contained many ig-negative lymphoid cells and, in the hindgut, also many large ig-positive macrophages, which appeared to bind ig. the lamina propria contained numerous ig-positive lymphoid cells next to ig-negative lymphoid cells and granulo ... | 2006 | 8449251 |
| plasmodium falciparum: the immune response in rabbits to the clustered asparagine-rich protein (carp) after immunization in freund's adjuvant or immunostimulating complexes (iscoms). | the plasmodium falciparum clustered asparagine-rich protein (carp) is a merozoite-associated antigen which contains approximately 30% asparagine. analysis of the dna sequences located 5' of the cloned 1.4-kb carp gene in the p. falciparum genome suggests that this gene fragment may encode the complete carp and that the gene product is a protein of m(r) 50,000. to analyze the immunogenicity of carp, the gene was expressed as a fusion protein with staphylococcal protein a (spa-carp). immunization ... | 1993 | 8454022 |
| filament-forming domain of carp dorsal myosin rod. | substructure of the myosin rod and its correlation to the filament formation were investigated by using fish myosin rod. it was found that fish rod contains a unique chymotrypsin susceptible site, 40 kda from the cooh-terminus or 20 kda downstream from the subfragment-2/light meromyosin junction (s-2/lmm junction). cleavage at this new site produced subfragment-2 possessing 95 kda subunit (95k s-2) and light meromyosin possessing 40 kda subunit (40k lmm). the latter is the shortest unit ever rep ... | 1993 | 8454573 |
| distribution of non-class-iii beta-tubulin isoforms in neuronal and non-neuronal cells. | beta-tubulin isoforms in brain tissues and in cell lines were analyzed by high-resolution isoelectric focusing in combination with monoclonal antibodies. post-translational modifications of brain non-class-iii beta-tubulin isoforms were found in phylogenetically distant species ranging from pig to carp. less extensive modifications were also observed in neuro-2a, hela and 3t3 cells, where most acidic isoforms were glutamylated, while the basic, most abundant isoforms were not. the data suggest p ... | 1993 | 8462682 |
| characterization of beta 2-microglobulin transcripts from two teleost species. | using degenerate primers based on published beta 2-microglobulin sequences we were able to obtain an expected 111 base pairs (bp) polymerase chain reaction (pcr) fragment from tilapia genomic dna. the sequence of this fragment showed a high degree of similarity to mouse beta 2-microglobulin at the protein level. we used these primers in an "anchored pcr" to obtain a 213 bp pcr fragment from a carp cdna library. this was then used to clone a full-length beta 2-microglobulin cdna from carp. the ca ... | 1993 | 8462991 |
| variable sensitivity of cyprinus carpio eggs, larvae, and fry to pesticides. | 1993 | 8467147 | |
| [the distribution of lernaea cyprinacea (copepoda: lernaeidae) in a crucian carp population]. | differences in the infection with copepods and the character of distribution of occurrence frequencies of lernaea in crucian carp depending on the body length and sex of the latter are shown. the lowest infection rate with copepods was recorded for fishes with body length 8-11 cm as compared to specimens with body length 12-18 and 18.5-34 cm. the two latter groups did not differ from each other in this index. in males and females whose body length is 8-11 cm as well as in females with body lengt ... | 2007 | 8474768 |
| an invertebrate calcium-binding protein of the calbindin subfamily: protein structure, genomic organization, and expression pattern of the calbindin-32 gene of drosophila. | antisera against vertebrate calcium-binding proteins cross-react with drosophila nervous and muscle tissue. we have used an antiserum against carp parvalbumin to isolate from a drosophila head cdna library immunopositive expression clones. tissue in situ hybridization identified a clone that labeled specific neurons and muscles similar to the parvalbumin-like immunohistochemical staining pattern. five independent cdnas derive from an mrna whose open reading frame codes for a 310 amino acid polyp ... | 1993 | 8478695 |
| pharmacokinetics of sulphadiazine and trimethoprim in carp (cyprinus carpio l.) acclimated at two different temperatures. | 1993 | 8478994 | |
| the deduced amino acid sequence of human carbonic anhydrase-related protein (carp) is 98% identical to the mouse homologue. | a recently reported mrna, encoding 'carbonic anhydrase-related polypeptide' (carp) from the purkinje cells of mouse cerebellum, was shown to have a 30-40% deduced amino acid sequence identity with the carbonic anhydrases (ca) of mammals. in order to compare the mouse and human carp sequences, we used the polymerase chain reaction (pcr) to amplify human carp sequences from several cdna libraries (salivary gland, testis and placenta). the sequence has an 89.3% sequence identity with mouse carp at ... | 1993 | 8482548 |
| new beta chain-encoding mhc class ii genes in the carp. | 1993 | 8482577 | |
| tempo and mode of mitochondrial dna evolution in vertebrates at the amino acid sequence level: rapid evolution in warm-blooded vertebrates. | by using complete sequence data of mitochondrial dnas, three markov models (dayhoff, proportional, and poisson models) for amino acid substitutions during evolution were applied in maximum likelihood analyses of mitochondrially encoded proteins to estimate a phylogenetic tree depicting human, cow, whale, and murids (mouse and rat), with chicken, frog, and carp as outgroups. a cow/whale clade was confirmed with a more than 99.8% confidence level by any of the three models, but the branching order ... | 1993 | 8483165 |
| isolation and primary structure of the three major forms of granulin-like peptides from hematopoietic tissues of a teleost fish (cyprinus carpio). | granulins are cysteine-rich polypeptides purified from human and rat hematopoietic cells and structurally related to the epithelin family of growth modulatory factors. a prototypic form of granulin was isolated from the hematopoietic organs of teleost fish (belcourt, d., and bennett, h.p.j. (1988) j. cell biol. 107, 629 (abstr.)). this study reports the structure of three granulins purified from the spleen and head kidney of the carp (cyprinus carpio). ion-spray mass spectrometric analysis of gr ... | 1993 | 8486624 |
| [the population level and distribution of ectoparasites in carp]. | the abundance and the distribution of dactylogyrus extensus and ichthyophthirius multifiliis on gills of two-years old carps have been investigated. it has been established that at least there are five groups, which differs from each other on the asymmetry of infection. | 1993 | 8488057 |
| presence of growth hormone receptors in carp liver and intestine. | membranes were prepared from tissues of the carp ctenopharynogodon idellus including liver, kidney, intestine, adipose tissue, ovary, gill, heart, muscle and spleen. the carp liver and intestine membranes bound 125i-labeled bovine growth hormone and the binding could be displaced by cold bovine growth hormone. no changes in the ability to bind 125i-labeled bovine growth hormone occurred after treatment of the carp liver membrane with dnase, rnase, alpha-amylase and beta-glucosidase, suggesting t ... | 1993 | 8490577 |
| characterization of catch-relaxing peptide (carp) immunoreactive neurons in the helix nervous system. | both small and large diameter of carp immunoreactive neurons could be observed in the different ganglia of cns of helix but not in the foot musculature. the immunoreactivity is the strongest in the varicose segments of immunoreactive fibers. the present findings suggest a transmitter or modulatory role of carp in both central and peripheral regulatory processes. | 1993 | 8493855 |
| risk factors for kidney cancer in new south wales. iv. occupation. | in a population based case-control study of kidney cancer in new south wales, data from structured interviews with 489 cases of renal cell cancer (rcc), 147 cases of renal pelvic cancer (carp), and 523 controls from the electoral roles were obtained about employment in certain industries or occupations, and exposure to particular chemicals chosen because of suspected associations with kidney cancer. a low level of education increased the risk for carp but not rcc. after adjustment for known risk ... | 1993 | 8494775 |
| intestinal, segmented, filamentous bacteria in a wide range of vertebrate species. | segmented, filamentous bacteria (sfbs) form a group of bacteria with similar morphology and are identified on the basis of their morphology only. the relationships of these organisms are unclear as the application of formal taxonomic criteria is impossible currently due to the lack of an in vitro technique to culture sfbs. the intestine of laboratory animals such as mice, rats, chickens, dogs, cats and pigs is known to harbour sfbs. to see whether this extends to other animal species, intestines ... | 1993 | 8501895 |
| determination of ichthiomycin concentration by biological test with cyprinus carpio l. | we have found out that one-year-old carps are extremely sensitive to ichthiomycin in concentrations between 25-125 micrograms/dm3. this fact permitted the use of biological methods for determination of ichthiomycin concentrations in cultural medium of streptomyces levoris 1107 or crude preparations of the antibiotic. | 1993 | 8511994 |
| efficacy of fumagillin against thelohanellus kitauei infection of israel carp, cyprinus carpio nudus. | the potential of fumagillin dicyclohexylamine salt to treat and prevent intestinal giant-cystic disease in israel carp, cyprinus carpio nudus, was monitored in field experimental studies. in experiment 1 (therapeutic), most fish were already naturally infected with more advanced stage of thelohanellus kitauei. fumagillin was administered to fish (mean body weight of 830 g) for a period of one month at a dose of 10.62 mg in the first group and 5.3 mg in the second group per fish per day. in exper ... | 1993 | 8512900 |
| gaba- and glycine-immunoreactive neurons in the spinal cord of the carp, cyprinus carpio. | gamma-aminobutyric acid (gaba) and glycine are the two main inhibitory transmitter amino acids in the central nervous system of vertebrates. the distribution of cells containing gaba and glycine in the carp spinal cord was examined by using specific antisera raised against the two amino acids conjugated to bovine serum albumin. the immunoreaction on serial paraffin sections was visualized by a streptavidin-biotin method. both antisera gave highly specific labelings of cells. at least three types ... | 1993 | 8514921 |
| [digestion, growth and body composition of carp (cyprinus carpio l.) after feeding different starch varieties]. | maize starch (treatment i), pregelatinized maize starch (ii), wheat starch (iii), pregelatinized wheat starch (iv), potato starch (v) or manioc meal (vi) were mixed in portions of 41% (i-v) or 46.7% (vi) to a basal diet. each experimental diet was fed to 33 carp (cyprinus carpio l.) (three aquaria with 11 carp each) in a daily amount of 1.8-2.0% of live weight. the experiment lasted 118 days and the carp had a mean live weight of 146 g at the beginning. the diets i-iv had a similar, high energy ... | 1993 | 8517771 |
| advanced rus and normal carpal bone age in childhood obesity. | skeletal maturation was evaluated in 60 prepubertal children aged 7.7 +/- 0.27 years (range 2.7-12.6). the radius, ulna and short bones (rus) and the carpal (carp) bone age were assessed. height corresponded to 1 +/- 0.1 z scores. | 1995 | 8520642 |
| an aquatic toxicological evaluation of fenthion in the context of finch control in south africa. | queletox, containing fenthion as active ingredient, is the avicide formulation used in south africa to control red-billed finches (quelea quelea). control measures involve night spraying of roosting areas with a light aircraft. since roosting areas often include reedbeds along riversides and on islands, proper control is difficult without exposing the aquatic environment to some risk of contamination. this study tested the acute effects of fenthion, in association with the queletox formulation, ... | 1995 | 8521783 |
| uptake and tissue distribution of dietary and aqueous cadmium by carp (cyprinus carpio). | the aim of this study was to assess whether contaminated prey can be a major source of cd for fish. for this purpose, the uptake and tissue distribution of dietary and aqueous cadmium by the carp (cyprinus carpio) was studied. the fish were exposed to either cd-contaminated food or cd-contaminated water for 4 weeks in laboratory experiments. when exposed to contaminated food, the fish were kept in clean water and fed contaminated chironomid larvae, equilibrated with 99 micrograms cd. liter-1. du ... | 1995 | 8521785 |
| localization, trafficking, and temperature-dependence of the aequorea green fluorescent protein in cultured vertebrate cells. | the localization, trafficking, and fluorescence of aequorea green fluorescent protein (gfp) in cultured vertebrate cells transiently transfected with gfp cdna were studied. fluorescence of gfp in uv light was found to be strongest when cells were incubated at 30 degrees c but was barely visible at an incubation temperature of 37 degrees c. cos-1 cells, primary chicken embryonic retina cells, and carp epithelial cells were fluorescently labeled under these conditions. gfp was distributed uniforml ... | 1995 | 8524871 |
| the effect of dietary energy and protein concentration and feeding level on feed utilization and body composition of carp (cyprinus carpio l.). | a three factorial designed feeding experiment with common carp (cyprinus carpio l.) was carried out in an intensive experimental rearing system. fish (initial body weight 200 g) were fed on two different levels of dietary energy (16 and 18 mj de/kg dm), two different levels of protein (320 and 420 g cp/kg dm) and also two different feeding intensities (100% and 75% of the maximum intake). the experiment was terminated when fish reached a mean body weight of 1300 g. growth, feed utilization and n ... | 1995 | 8526728 |
| retraction of spinule-type neurites from carp retinal horizontal cell dendrites during dark adaptation involves the activation of ca2+/calmodulin-dependent protein kinase ii. | the formation of spinules at the terminal dendrites of retinal horizontal cells with the onset of light and their subsequent retraction during darkness is a remarkable example of synaptic plasticity where sensory experience modifies reversibly, and on a time scale of minutes the ultrastructure of synaptic connectivity. the signals and the subsequent intracellular cascades underlying the prominent morphological alterations are only partially understood. we show here that lowering the external cal ... | 1995 | 8528466 |
| genetic variation in susceptibility to trypanoplasma borreli infection in common carp (cyprinus carpio l.). | gynogenetic reproduction of homozygous females, or crossbreeding two homozygous animals, results in fish lines without genetic variation. hybrid crosses are expected to express a more stable development than homozygous lines, the latter may have an important value for gaining insight into genetic components of host resistance to parasite infection. we examined the antibody response of carp (cyprinus carpio l.) to infection with trypanoplasma borreli. outbred carp responded with a production of s ... | 1995 | 8533293 |
| a new fluorochromasia method using a fluorescence microplate reader for assay of cytotoxic activity of carp leucocytes. | a new fluorochromasia method using a fluorescence microplate reader has been established to assay spontaneous cytotoxic activity of carp leucocytes. this method is characterized by using propidium iodide (pi) for staining dead target cells and a fluorescence microplate reader for measurement of the fluorescence of pi. k562 as target cells were prepared in 96-well flat-bottom microplates, and carp leucocytes were added as effector cells. after 2.5 h incubation, pi was added to each well. after an ... | 1995 | 8533295 |
| [high molecular weight forms of aminoacyl-trna synthetases in animals. ii. high molecular weight aminoacyl-trna synthetase complexes in lower animals]. | high-molecular-weight aminoacyl-trna synthetase complexes have been found in hen, frog and carp liver cytosol as well as in cytosol from snails and paramecium. the complexes from vertebrates and snails contained 13-15 aminoacyl-trna synthetases, revealed mass of about 4,000 kda and sedimentation constant about 19 s. the complex from paramecium containing 11 aminoacyl-trna synthetases, revealed a mass of about 1,600 kda and sedimentation constant 15 s. the results of the present study support the ... | 1993 | 8534161 |
| evidence that the inhibitory effects of stress on reproduction in teleost fish are not mediated by the action of cortisol on ovarian steroidogenesis. | ovarian follicles of goldfish, common carp, and the sparid pagrus auratus (new zealand snapper) were incubated in vitro to assess the effects of cortisol (f) on ovarian steroidogenesis. unstimulated goldfish follicles produced little testosterone (t) or 17 beta-estradiol (e2), whereas both carp and snapper follicles spontaneously produced e2 and to a lesser extent t. goldfish follicles produced increased amounts of e2 in response to treatment with human chorionic gonadotropin (hcg), whereas carp ... | 1995 | 8536936 |
| somatostatin-, vasoactive intestinal peptide-, and granulin-like peptides isolated from intestinal extracts of goldfish, carassius auratus. | three new peptides were originally isolated from intestinal extracts of goldfish. they were structurally related with somatostatin, vasoactive intestinal peptide (vip), and granulin (grn) and thus termed goldfish somatostatin (gss-28), gvip, and ggrn, respectively. the primary structures of these peptides were determined as: svessnhlpa 10rerkagcknf20ywkgftsc for the gss-28; hsdavftdny10sryrkqmaak20kylnsvla-nh2 for the gvip, and vihcdsstic10 pdgttcclsp20ygvwyccpfs30mgqccrdgih40ccrhgyhcds50tsthclr ... | 1995 | 8536941 |
| studies on the structure and function of the carp gonadotropin alpha subunit by site-directed mutagenesis. | there are two genes encoding the alpha subunit of carp gonadotropin (alpha 1 and alpha 2 subunits). our previous data have demonstrated that both alpha 1 and alpha 2 subunits expressed in insect cells are able to associate with the beta subunit, but only the alpha 1/beta heterodimer displays biological activity. in the mature protein, there are only four amino-acid residues different between the alpha 1 and alpha 2 subunits. in this study we used site-directed mutagenesis and expressed different ... | 2006 | 8537173 |
| [the perceptive brightness space in the carp (carpio cyprinus l.)]. | brightness discrimination of black-white (in the range of 0.0082-0.214 w/m2; cie chromaticity coordinates x = 0.340, y = 0.354) and red (in the range of 0.0035-0.106 w/m2; cie chromaticity coordinates x = 0.641, y = 0.342) stimuli was studied during using choice conditioning paradigm in two carps. confusion matrices for black-white and red stimuli separately were constructed from probabilities of instrumental responses (catching of a bead) to conditioned and differential stimuli in sequential si ... | 2006 | 8560943 |
| toxic effects, at three ph levels, of two reference molecules on common carp embryo. | early life-stage survival, motility, and growth toxicity tests were carried out on common carp (cyprinus carpio l.) endotrophic embryo, with two reference molecules (captafol and dnoc), from fertilization to the end of the first two-thirds period of mortalities by starvation. thirteen days duration exposure was performed in daily renewed medium, at 24.5 degrees c, in standard synthetic water, at three phs (6.9, 7.8, 9.0), in the presence of 10 mm/liter nontoxic ph buffer. nominal concentrations ... | 1995 | 8565873 |
| dermocystidium koi found in skin lesions in koi carp (cyprinus carpio). | 1995 | 8571508 | |
| a mother and three daughters with congenital dislocation of the hip and a characteristic facial appearance: a new syndrome? | we describe a mother and her three daughters who all have bilateral congenital dislocation of the hip. the mother has had no other medical problems and is on the 90th centile for height. her three daughters resemble each other strongly with facial characteristics which include hypertelorism, epicanthic folds, puffiness around the eyes, a flat mid-face and a carp-shaped mouth. all three daughters are on the 3rd centile for height, with their head circumference on a higher centile and all had an a ... | 1995 | 8574416 |
| alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp. | monitoring genotoxicity of the environment using endemic organisms as sentinels requires the development of sensitive assays. toward this end, we explored the feasibility of applying the alkaline single cell gel (scg) or "comet" assay. this approach involves detection, under alkaline conditions, of cell dna fragments which, on electrophoresis, migrate from the nuclear core, resulting in a "comet with tail" formation. tail length has been correlated with level of genotoxicant exposure in a number ... | 1995 | 8575424 |
| investigations into the ubiquitous nature of high or low immune responsiveness after divergent selection for antibody production in common carp (cyprinus carpio l.). | this paper reports on the selection of individual carp with a high or low antibody response, in combination with reproduction by gynogenesis, in order to develop well-characterised inbred carp lines consisting of practically unlimited numbers of carp with the same genotype. two homozygous progenies, previously characterised as having a high or low immune response to dinitrophenyl keyhole limpet haemocyanin (dnp-klh), were immunised with either a t-dependent (dnp-human serum albumin (dnp-hsa)) or ... | 1995 | 8578693 |
| effect of potassium permanganate, cobalt chloride and vitamin b complex on the haematological parameters of cadmium treated common carp, cyprinus carpio. | effect of potassium permanganate, cobalt chloride and vitamin b complex on the haematological parameters of common carp cyprinus carpio were markedly influenced by a treatment of 2.5 mg/l cd in the laboratory for 96 hours. hb% and esr values were increased but the rbc and pcv values were reduced. treatment of kmno4 (1 mg/l) or cocl2 (2 mg/l) induced a further reduction of rbc of the cd treated fish. but parameters like pcv, mcv and mch of cd treated fish were not affected by the treatment of kmn ... | 1995 | 8580524 |
| eighteen years of vaccination against viral haemorrhagic septicaemia in france. | viral haemorrhagic septicaemia (vhs) has been considered for many years to be a major cause of loss in the french trout industry. the high prevalence of vhs in certain geographic areas made a control strategy based on control policy unfeasible. this provided the impetus for immunoprophylaxis development that resulted in 3 successive types of vaccines: inactivated, live attenuated and recombinant vaccines. when delivered by intraperitoneal injection, the 2 propiolactone-inactivated vhs virus was ... | 1995 | 8581010 |
| comparison of the polymerases (l genes) of spring viremia of carp virus and infectious hematopoietic necrosis virus. | we have determined the partial nucleotide sequences of the polymerase genes of the fish rhabdoviruses, spring viremia of carp virus (svcv) and infectious hematopoietic necrosis virus (ihnv). at this point we have deduced the amino acid sequences and analysed the first 1,400 amino acids comprising two thirds of the polymerase genes of svcv and ihnv. we have compared sequence similarities of svcv and ihnv polymerases with other rhabdovirus and paramyxovirus polymerases. the svcv polymerase showed ... | 1995 | 8581012 |
| detection of spring viremia of carp virus by hybridization with biotinylated dna probes. | 1995 | 8581035 | |
| the complete nucleotide sequence of the mitochondrial dna genome of the rainbow trout, oncorhynchus mykiss. | the complete nucleotide sequence of the mitochondrial dna of the rainbow trout, onchorynchus mykiss, has been determined. the total length of the molecule is 16,660 bp. the rainbow trout mitochondrial dna has the same organization described in eutherian mammals, the clawed frog (xenopus laevis), and the two fish species, oriental stream loach (crossotoma lacustre) and carp (cyprinus carpio). alignment and comparison of the deduced amino acid sequences of the 13 proteins encoded by rainbow trout ... | 1995 | 8587139 |
| regional distribution of vasoactive intestinal peptide immunoreactivity in the brain of salmon, trout and carp. | the content of vasoactive intestinal peptide (vip) was determined by radioimmunoassay in different brain regions of three fish species, namely salmon, trout and carp. the vip extracted from salmon brain was shown by reverse-phase high-performance liquid chromatography to coelute with native porcine vip. highest vip levels were measured in the salmon brain with a maximum of 112 pg/mg protein in the hypothalamus, followed by the telencephalon, olfactory bulb and optic tectum. in contrast, in the t ... | 2006 | 8590917 |
| [light and electron microscopic studies on the sporogenesis of sphaerospora molnari in the gill lamellas of carp (cyprinus carpio)]. | the sporogenesis of sphaerospora molnari in the gill lamellas of the common carp was investigated by light- and electron microscopy. whereas pseudoplasmodia were not identified, the development from one secondary cell containing two tertiary cells to the mature spore was pursued. monosporic sporoblasts surrounded by an envelope cell were observed lying isolated in the host tissue. sporoblast generative cells differentiated into two capsulogenic cells, two mononucleate sporoplasms and two valvoge ... | 1995 | 8593138 |
| insulin/igf-i binding ratio in skeletal and cardiac muscles of vertebrates: a phylogenetic approach. | insulin and insulin-like growth factor (igf-i) receptor binding and tyrosine kinase activity were characterized in cardiac and skeletal muscles of several vertebrates. specific insulin binding per unit weight of skeletal muscle was clearly higher in pigeon and rat than in ectothermic vertebrates (32 +/- 5 and 25 +/- 2.7%/100 mg initial tissue in pigeon and rat, respectively, vs. 4.4 +/- 0.2%/100 mg in carp samples). insulin binding clearly predominated over igf-i binding in skeletal muscle of en ... | 1995 | 8594939 |
| development of an in vitro model of essential fatty acid deficiency in fish cells. | levels of eicosapentaenoic acid (epa; 20:5, n-3) greatly exceed those of arachidonic acid (aa; 20:4, n-6) in the tissue phospholipids of most fish species. despite this, it is 20:4, n-6-derived eicosanoids that are produced predominantly in fish cells. the development of an essential fatty acid (efa)-deficient fish cell line would greatly assist the study of this selectivity and so several fish cell lines were cultured in efa-deficient (efad) media. all n-3 and n-6 polyunsaturated fatty acids (p ... | 1995 | 8596777 |
| identification and characterization of a new major histocompatibility complex class i gene in carp (cyprinus carpio l.). | in this study we report the finding of three representatives of a new group of major histocompatibility complex class i sequences in carp: cyca-12 (cyca-ua1*01), a full-length cdna; cyca-sp1 (cyca-uaw1), a polymerase chain reaction (pcr) fragment from cdna; and cyca-g11 (cyca-ua1*02), a partial genomic clone. comparison of the amino acid sequences of cyca-12, cyca-sp1, and cyca-g11 with classical and non-classical class i sequences from other species shows considerable conservation in regions th ... | 1996 | 8613142 |
| molecular architecture and biophysical properties of phospholipids during thermal adaptation in fish: an experimental and model study. | phospholipids from livers of carps (cyprinus carpio l.) adapted to winter (5 degrees c) and summer (25 degrees c) temperatures were isolated, and the fatty acid composition of total phospholipids, as well as molecular species composition of diacyl phosphatidylcholines and ethanolamines, were determined. order parameter of 5-doxyl stearic acid and steady-state fluorescence anisotropy of different anthroyloxy fatty acids--[2-, 12(n-9-anthroyloxy)stearic acid and 16(n-9-anthroyloxy)palmitic acid--e ... | 1995 | 8614302 |
| neutrophilic granulocytes in carp, cyprinus carpio, possess a spontaneous cytotoxic activity. | this study demonstrates for the first time that carp (cyprinus carpio) neutrophilic granulocytes from the head kidney possess potent spontaneous cytotoxic activity against several human tumor cell lines. carp head kidney cells isolated at a density of 1.09 g/ml contained more than 90% neutrophilic granulocytes. these cells were round and approximately 10 millimicrons in diameter with reniform or polymorphic nuclei and slightly eosinophilic cytoplasm when stained with giemsa. electron microscopy ... | 1995 | 8617402 |
| cold-induced expression of delta 9-desaturase in carp by transcriptional and posttranslational mechanisms. | poikilothermic animals respond to chronic cold by increasing phosphoglyceride unsaturation to restore the fluidity of cold-rigidified membranes. despite the importance of this compensatory response, the enzymes involved have not been clearly identified, and the mechanisms that control their activity are unknown. in carp liver, cold induces an 8- to 10-fold increase in specific activity of the microsomal stearoyl coenzyme a desaturase. cold-induced up-regulation of gene transcription resulted in ... | 1996 | 8629000 |
| genomic structure of carp mitogen-activated protein kinase kinase 1 gene. | carp mitogen-activated protein kinase kinase 1 (cmkk1) gene was isolated from a liver genomic library. the sequence around the exon-intron boundaries and 2 kb of the promoter region were determined. our data indicate that this gene is composed of 11 exons and 10 introns spanning about 9 kb. multiple potential transcription initiation sites were located by primer extension analysis. examination of 2 kb of 5'-flanking sequence revealed potential binding sites for a variety of transcription factors ... | 1996 | 8634328 |
| spermatozoa: models for studying regulatory aspects of energy metabolism. | spermatozoa are highly specialized cells, and they offer advantages for studying several basic aspects of metabolic control such as the role of adenosine triphosphate-(atp)-homeostasis for cell function, the mechanisms of fatigue and metabolic depression, the metabolic channelling through the cytoplasm and the organization and regulation of glycolytic enzymes. spermatozoa of four species with different reproductive modes are introduced and the first results are presented: spermatozoa of the mari ... | 1996 | 8641386 |
| isolation and characterization of two distinct growth hormone cdnas from the goldfish, carassius auratus. | as a first step toward the development of a ribonuclease protection assay for studying the regulation of growth hormone (gh) gene expression in pituitary cells of the goldfish, carassius auratus, we report the isolation of two cdna clones encoding goldfish gh from a cdna library prepared from pituitary poly(a)+ rna. the complete nucleotide sequences of these two gh cdna clones have been determined and both of them were predicted to encode a polypeptide of 210 amino acids (aa) including a putativ ... | 1996 | 8651695 |
| the influence of water potassium concentration on 137cs excretion from fish. | results are reported of the investigation on the peculiarities of 137cs release from carp (cyprinus carpio l.) acclimatized to different potassium concentrations in water. the dynamics of radiocesium release are characterized by slow and fast components. the 137cs release rates observed in the experiments with different water potassium concentrations were not markedly different from the point of view of middle-term radioecological predictions. | 1996 | 8655326 |
| the innervation of the chromaffin cells in the head kidney of the carp, cyprinus carpio; regional differences of the connections between nerve endings and chromaffin cells. | nerve fibres and their connections with chromaffin cells in the carp head kidney were studied by light and electron microscopy. some nerve bundles entered the head kidney from the dorsal aspect along veins. many unmyelinated axons emerged from the nerve bundles to invade the clusters of chromaffin cells, the distribution of which was restricted to the neighbourhood of the venous trunks and their tributaries. most of the nerve endings were attached to a chromaffin cell by synaptic junctions and w ... | 1996 | 8655401 |
| acute effect of organotin compounds to red sea bream and red carp using biological parameters. | 1996 | 8661861 | |
| characterization of class ii a and b genes in a gynogenetic carp clone. | a prerequisite for carrying out functional studies on major histocompatibility complex (mhc) molecules of fish is the availability of genetically well-defined homozygous strains. previously we have applied gynogenetic reproduction to generate isogenic carp, denoted clone a410. this clone has recently been demonstrated to express a single class i gene, cyca-ua1(*)01, and in the present study two class ii b and two class ii a transcripts were obtained. the two class ii b transcripts, cyca-d(cb3)b ... | 1996 | 8662086 |
| the characterisation of the 5' regulatory region of a temperature-induced myosin-heavy-chain gene associated with myotomal muscle growth in the carp. | we have isolated and characterised the 5' region of a member of the carp myosin heavy chain gene family. expression of this gene has previously been shown to be induced by an increase in environmental temperature and is restricted to the small-diameter white myotomal muscle fibres which are associated with growth. the whole isoform gene, including potential regulatory sequence 5' to the transcription start site and the 3' untranslated region was cloned in a lambda2001 bacteriophage vector. studi ... | 1996 | 8665910 |
| microcalorimetric study of mitochondria isolated from fish liver tissue. | we determined the thermogenesis curves of mitochondria isolated from fish liver tissue by using an lkb 2277 bioactivity monitor. after isolation from the fish liver, mitochondria still have activity and can live for a long time by using the stored nutrients. we calculated the recovery rate constants of mitochondria. we found that the thermogenesis curves of mitochondria are similar to those obtained from prokaryotic cells, but not similar to those obtained from eukaryotic cells. we determined th ... | 1996 | 8675962 |
| in vitro polarization of carp leucocytes in response to the blood fluke sanguinicola inermis plehn, 1905 (trematoda: sanguinicolidae). | an in vitro assay was used to determine the effects of sanguinicola inermis adults and cercariae on the polarization responses of pronephric leucocytes of carp. leucocytes were isolated and exposed to live adult flukes or cercariae for up to 48 h. differences in polarization responses were related to the presence of the parasite, the presence or absence of carp serum and the time of incubation. the mean proportions of cells exhibiting polarization in unstimulated controls ranged from 5 to 30% ov ... | 1996 | 8677139 |
| insulin-like growth factor iea2 is the predominantly expressed form of igf in common carp (cyprinus carpio). | an insulin-like growth factor (igf) complementary dna (cdna) was isolated from a liver cdna library of adult common carp, cyprinus carpio. the identity of this cdna clone was confirmed by nucleotide sequence determination. sequence comparison with other vertebrate and rainbow trout igfs showed that this cdna encodes a particular subtype of igf-i, igf-iea2. a reverse transcription-polymerase chain reaction (rt-pcr) assay showed that this igf-i is expressed mainly in the liver (hepatopancreas) of ... | 1996 | 8680527 |
| a tgacg motif mediates growth-hormone factor-1/pituitary-transcriptional-activator-1-dependent camp regulation of the rainbow trout growth-hormone promoter. | the mechanisms involved in the regulation of the rainbow trout growth hormone (tgh) gene promoter by the pituitary-specific transcription factor ghf1 (growth hormone factor 1), also called pit1 (pituitary transcriptional activator 1), and camp have been investigated in mammalian and fish cells. the -340 to +24 5'-flanking fegion of the tgh gene focused to the luciferase gene was activated in rat pituitary gc cells and in hela cells cotransfected with an effector plasmid encoding rat ghfi. gc cel ... | 1996 | 8706656 |
| effects of different patterns of light adaptation on cellular and synaptic plasticity in teleost retina: comparison of flickering and steady lights. | the importance of the pattern of light stimulus in inducing light-adaptive morphological (cellular and sub-cellular) changes in the outer retina of the carp was assessed. thus, the effects of steady and flickering backgrounds (of the same intensity) on cone photomechanical movements (pmms) and horizontal cell (hc) spinules were compared by quantitative measurements. for both phenomena, flickering stimuli had a significantly greater effect than steady, although the number of photons delivered by ... | 1996 | 8710195 |
| effects of an organophosphate on the antioxidant systems of fish tissues. | the effect of a hungarian made and applied insecticide, organophosphate (dichlorvos) on antioxidant enzymes and other oxidative and redox parameters of two different fish species, carp (cyprinus carpio l.) and catfish (ictalurus nebulosus) was studied. the two fish species have different ways of life. from antioxidant enzymes, changes in superoxide dismutase, catalase, glutathione peroxidase and, in the case of carp, acetylcholinesterase activities were studied in tissue homogenates. other param ... | 1995 | 8714762 |
| biochemical and histopathological effects of glyphosate on carp, cyprinus carpio l. | glyphosate, also known by the trade names roundup and rodeo for agricultural use, is a broad-spectrum, translocated herbicide, used primarily in agricultural applications, and for vegetation control in non-crop areas. it is used as non-selective herbicide and for aquatic weed control in fish-ponds, lakes, canals, slow running water, etc. (usda 1984). glyphosate is perhaps the most important herbicide ever developed. literature of toxicological and ecotoxicological properties of glyphosate is ext ... | 1996 | 8720103 |
| electrocardiographic characterization of myocardial function in normoxic and hypoxic teleosts. | electrocardiography was applied to analyze cardiac function of four teleost species (piaractus mesopotamicus, hoplias malabaricus, hoplias lacerdae and cyprinus carpio) during normoxia and graded hypoxia. in these species, hypoxic bradycardia consistently occurred during severe hypoxia (below the critical oxygen tension--pco2) and was accompanied by alterations in the ecg recordings. three basic ecg alterations were demonstrable: 1) increase in the t wave area and amplitude, being more positive ... | 2016 | 8728858 |
| further evidence on the interaction of muramyl dipeptide with the serotonergic system. | the mode of interaction between muramyl dipeptide (mdp), a compound with immunopharmacological activities, and 5-hydroxtryptamine (5-ht, serotonin) was studied in isolated nerve-smooth muscle preparations of the carp stomach. application of exogenous 5-ht evoked direct smooth muscle contractions; electric neurogenic stimulation evoked twitches due to release of 5-ht from nerve endings. contractions evoked by a high concentration of 5-ht (3-30 microm) were resistant to atropine and potentiated in ... | 1996 | 8732429 |
| effects of microcystin-lr on the partial reactions of the na(+)-k+ pump of the gill of carp (cyprinus carpio linneo). | several partial reactions of the na(+)-k+ pump enzyme were studied in a microsomal fraction derived from the gill of carp (cyprinus carpio linneo). we tested the effect of three toxins [(i) microcystin-lr, (ii) microcystin-lr-like toxin component isolated from microcystis aeruginosa culture and (iii) okadaic acid] on the phosphorylation, ouabain binding and atpase activity of the na(+)-k+ pump. the k(+)-dependent hydrolysis of the na(+)-dependent phosphorylation of na(+)-k+ pump, as well the rel ... | 1996 | 8735244 |
| identifiable achatina giant neurones: their localizations in ganglia, axonal pathways and pharmacological features. | 1. an african giant snail (achatina fulica férussac), originally from east africa, is now found abundantly in tropical and subtropical regions of asia, including okinawa in japan. this is one of the largest land snail species in the world. the achatina central nervous system is composed of the buccal, cerebral and suboesophageal ganglia. the 37 giant neurones were identified in these ganglia by the series of studies conducted over about 20 years. the identifications were made by the localization ... | 1996 | 8742492 |
| structural modifications of non-mammalian gonadotropin-releasing hormone (gnrh) isoforms: design of novel gnrh analogues. | three natural forms of vertebrate gonadotropin-releasing hormone (gnrh) provided the structural basis upon which to design new gnrh agonists: [his5,trp7,leu8]-gnrh, dogfish (df) gnrh; [his5,asn8]-gnrh, catfish (cf) gnrh; and [his5,trp7,tyr8]-gnrh, chicken (c) gnrh-ii. the synthetic peptides incorporated the position 6 dextro (d)-isomers d-arginine (d-arg) or d-naphthylalanine (d-nal) in combination with an ethylamide substitution of position 10. the in vitro potencies for lh and fsh release of t ... | 1995 | 8746537 |
| identification of pk-a phosphorylation sites in the carboxyl terminus of l-type calcium channel alpha 1 subunits. | full length l-type calcium channel alpha 1 subunits are rapidly phosphorylated by protein kinase a (pk-a) in vitro and in vivo at sites located in their long carboxyl terminal tails. in skeletal muscle, heart, and brain the majority of biochemically isolated alpha 1 subunits lacks these phosphorylation sites due to posttranslational proteolytic processing. truncation may therefore modify the regulation of channel activity by pk-a. we combined site-directed mutagenesis and heterologous expression ... | 1996 | 8755718 |
| comparison of dark- and light-adapted carp retinas with nadph diaphorase staining. | the carp retina was examined by nadph diaphorase histochemistry to determine if the staining pattern of retinal cells was changed depending on the adaptation state of the retina. when dark-adapted for 5 h, ellipsoids of inner segments of both rods and cones and some horizontal cells were heavily stained. staining was also found in subpopulations of amacrine cells and ganglion cells. in addition, müller cells were strongly positive for nadph diaphorase. when light-adapted for 5 h, ellipsoids of p ... | 1996 | 8760454 |
| cobalt ions enhance light responsiveness of carp cone horizontal cells in low calcium. | effects of cobalt ions (co2+) on horizontal cells in low extracellular calcium were examined in isolated, superfused carp retinas. while 0.1 mmol/l co2+ completely suppressed both rod- and cone-driven horizontal cells in normal ringer's solution, it enhanced light responses of cone horizontal cells in low (0.1 mmol/l) calcium. the enhancement of the cone horizontal cell response by co2+ was not caused by changes in light responsiveness of cone photoreceptors. moreover, application of 50 mumol/l ... | 1996 | 8760455 |
| an isothermal induction of delta 9-desaturase in cultured carp hepatocytes. | cold exposure of carp leads to the induced activity of the hepatic delta 9-desaturase (schünke, m. and wodtke, e. (1983) biochim. biophys. acta 734, 70-75). we have investigated the controlled expression of this enzyme using isolated carp hepatocytes. culture at 30 degrees c, of cells isolated from 30 degrees c-acclimated carp. resulted in an 8-13-fold increase in desaturase-specific activity over 4 days, whilst another enzyme of intermediary metabolism, glucose-6-phosphatase, decreased by more ... | 1996 | 8765141 |
| estimation of essential fatty acid requirements of common carp larvae using semi-purified artificial diets. | two trials were conducted with duplicate groups of (first feeding) carp larvae fed artificial dry diets based on casein and dextrin over 21 or 25 days. one control diet based on yeast was also tested. survival, growth and fatty acid profiles of larvae were studied. in trial 1, (n-3) fatty acid requirement was estimated using diets supplemented or not with methyl linolenate or cod liver oil. after 21 days, the best survival and growth were observed in larvae fed the unsupplemented diet [(n-3) fat ... | 1996 | 8766972 |
| role of membrane lipids in temperature acclimatization of carp. | 1996 | 8766974 | |
| influence of dietary fatty acid composition and vitamin e on fatty acids and alpha-tocopherol in carp (cyprinus carpio l.). | this paper examines the effect of different fatty acid composition, together with varying dietary vitamin e contents on fatty acid composition and tocopherol and tocotrienol contents of carp, and compares with other species of fish and the storage quality of the fish produced. dietary fatty acid composition affected fatty acid patterns of tissues of carp significantly, but this was correlated with the fat content of the diet. varying the vitamin e content results in no or only very slight variat ... | 1996 | 8766975 |
| dietary effects on fatty acid metabolism of common carp. | the paper summarises experimental data demonstrating effects of various dietary factors exerting changes in the fatty acid composition and fatty acid metabolism of the common carp (cyprinus carpio l.). among the dietary factors (1) supplementary feeding in fish ponds, (2) absence of essential fatty acids (efa) in the diet, (3) starvation, and (4) ration level were studied. it was concluded that supplementary feeding in carp rearing ponds is frequently excessive in the hungarian carp culture prac ... | 1996 | 8766976 |
| protein sparing effect and nutritive significance of lipid supplementation in carp diets. | although carp is in a position to utilize carbohydrates efficiently, lipids are an important energy source in carp diets. in general fat-enriched high-energy diets result in rapid growth and favourable feed conversion. many authors emphasize the protein-sparing effect of lipid supplementation in carp diets. for efficient protein-utilization an optimal protein/energy ratio in feed is necessary. good results may be obtained with diets containing 30-50% high-quality protein, > 14 mj digestible ener ... | 1996 | 8766977 |
| evidence of sensory conflict and recovery in carp exposed to prolonged weightlessness. | evidence in support of the sensory conflict hypothesis for space motion sickness (sms) is still needed. | 1996 | 8775405 |
| antibody against pulmonary surfactant protein a recognizes proteins in intestine and swim bladder of the freshwater fish, carp. | an antibody raised against rat pulmonary surfactant protein a (sp-a) bound on western blots to proteins present in intestinal mucosa and in swim bladder, but not in gills of the carp. the fish protein(s) revealed by the antibody exhibited an electrophoretical behavior similar to that of rat sp-a with a characteristic triplet in the 30- to 35-kda range. it therefore appears that proteins immunologically very close to mammalian sp-a are present in modern fish which evolved from ancestors that neve ... | 1996 | 8780709 |
| corticotropin-releasing hormone (crh) receptors in the mesenteric small arteries of rats resemble the (2)-subtype. | the potencies of the corticotropin-releasing hormone (crh) agonistic peptides ocrh, h/rcrh, frog sauvagine, and carp urotensin i and of the antagonistic peptide alpha-helical crh9-41 were compared in 3 different in vitro assays: (a) receptor binding to rat brain membranes; (b) release of acth/beta-endorphin from rat pituitary cells; and (c) relaxation of rat mesenteric small arteries. from their potency profiles, especially from the high potency of sauvagine relative to crh in the relaxation ass ... | 1996 | 8781499 |
| fast neutralization/immunoperoxidase assay for viral haemorrhagic septicaemia with anti-nucleoprotein monoclonal antibody. | an enzyme-immunohistochemical procedure was employed to facilitate neutralization/diagnostic tests for viral haemorrhagic septicaemia virus (vhsv), a significant pathogen in trout farms throughout europe. the method described can be used for trout or mice antibodies; increases speed (1 day), simplicity, and minimizes the use of reagents compared to other neutralization assays. furthermore, the test requires a minimum handling of the cell cultures under sterile conditions, decreasing frequent con ... | 1996 | 8783145 |
| nuclear sm antigens in the sperm of different organisms. | immunoblot analysis of sperm protein from several species revealed the presence of polypeptides recognised by anti-sm sera obtained from patients with systemic lupus erythematosus. immunoreactive polypeptides in human, bull, mouse and rat sperm were identified as protein b', b and d as compared with the sm polypeptides of hela cells. in the sperm of rooster, the teleost fish cyprinus carpio and the mussel choromytilus chorus, the immunoreactive polypeptide profile was more complex. to ascertain ... | 1994 | 8785681 |
| vertebrate heart development. | this review summarizes recent studies of the cellular and molecular events involved in the determination and differentiation of cardiac myocytes in vertebrate embryos. fate-mapping studies in mouse, chick, amphibian and zebrafish embryos suggest that cardiac muscle precursors are specified shortly before or at the time of gastrulation. nuclear factors, such as dhand, aryl hydrocarbon receptor, gata-6, nkx-2.3, growth arrest homeobox (gax) and cardiac adriamycin responsive protein (carp), which h ... | 1996 | 8791539 |
| relative susceptibility of different life stages of channa punctatus and cyprinus carpio to nickel-chrome electroplating effluent. | 1996 | 8791562 | |
| carp reproduction in highly eutrophic pond conditions. | 1996 | 8791563 | |
| expression of hoxb-1 during gastrulation and segmentation stages of carp (cyprinus carpio). | this report describes the cdna sequence and embryonic rna expression pattern of carp hoxb-1. carp hoxb-1 is a labial-like, homeobox-containing gene of the 3' end of the hox gene cluster. the expression pattern in carp is compared to that of homologs in other vertebrates. as holds for other hox genes, carp hoxb-1 is expressed with highest intensity at a sharp anterior boundary, and expression fades out towards posterior. at later stages, gaps were found in the domain. the gene is expressed from l ... | 1996 | 8793616 |
| multiple regulation of carp (cyprinus carpio l.) macrophages and neutrophilic granulocytes by serum factors: influence of infection with atypical aeromonas salmonicida. | normal carp serum contains inhibitory and stimulatory factors for macrophage and neutrophilic granulocyte respiratory burst activity. as stimulatory factors were only effective in combination with phorbol myristate actetate (pma) activation, it is concluded that they are probably linked to protein kinase c activation. both the stimulatory and inhibitory factors are heat stable. macrophage- and neutrophilic granulocyte-enriched cell fractions from the pronephros of carp had high respiratory burst ... | 1996 | 8797288 |
| inhibition of cytotoxic activity of carp lymphocytes (cyprinus carpio) by anti-thymocyte monoclonal antibodies. | a series of monoclonal antibodies (mabs) were developed against carp thymocytes and carp immunoglobulin (igm). anti-thymocyte mabs reacted with thymocytes, head kidney lymphocytes (hkl) as well as peripheral blood lymphocytes (pbl) at various proportions. anti-igm antibody reacted with hkl and pbl but not with thymocytes. pbl from carp immunized with xenogeneic mouse myeloma cells exhibited cytotoxic activity against target cells but pbl from unimmunized fish showed weak cytotoxicity. treatment ... | 1996 | 8797290 |
| characterization and ion channel activities of novel antibacterial proteins from the skin mucosa of carp (cyprinus carpio). | a detergent-solubilized fraction of skin mucus of carp (cyprinus carpio) induced ion channels after reconstitution into planar lipid bilayers. a differential extraction using a non-ionic detergent followed by electrophoretic separation led to the isolation of two hydrophobic 31-kda and 27-kda proteins. in contrast to the 27-kda protein, which was glycosylated, the 31-kda did not bind to concanavalin a. the reconstitution of these proteins into a planar lipid bilayer restored the ionophore behavi ... | 1996 | 8797847 |
| detection of mhc class ii transcripts in lymphoid tissues of the common carp (cyprinus carpio l.). | in all vertebrates studied to date, the expression of mhc class ii genes is known to be restricted to a limited number of tissues and cell types. in order to have a better understanding of the function of the equivalent genes in teleost fish, the distribution of mhc class ii beta transcripts (cyca-dab) in the common carp (cyprinus carpio l.) was investigated. rna was isolated from tissues and leucocytes, cdna was produced, and amplification of the cyca-dab genes was carried out by pcr. of the or ... | 2006 | 8801130 |
| development of a radioimmunoassay for european eel growth hormone and application to the study of silvering and experimental fasting. | a specific and sensitive homologous radioimmunoassay for eel (anguilla anguilla l.) growth hormone (anggh) has been developed. the antiserum, raised against purified anggh and used at 1:20,000 final dilution, did not cross-react with eel prolactin or thyrotropin, carp gonadotropin ii, bovine gh, or serum from hypophysectornized eel. the inhibition curves for eel pituitary extracts and serum were parallel to that of anggh standard. the ed50 value was between 1 and 2 ng/tube and the recovery of pu ... | 1996 | 8804566 |