Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| schistosoma japonicum: protective immunity induced by schistosomulum-derived cells in a mouse model. | we previously reported that immunization with intact live cells from schistosomula of schistosoma japonicum (s.j) partially protected the kunming strain of mice from challenge infection. in the present work, 2 immune protective experiments were designed to further validate the protective effect induced by this type of vaccine and to optimize the immunization protocol, including the number of inoculations and parasite stages from which immunogenic cells were derived. three antigens derived from 1 ... | 2008 | 18564740 |
| sjtpdb: integrated transcriptome and proteome database and analysis platform for schistosoma japonicum. | schistosoma japonicum is one of the three major blood fluke species, the etiological agents of schistosomiasis which remains a serious public health problem with an estimated 200 million people infected in 76 countries. in recent years, enormous amounts of both transcriptomic and proteomic data of schistosomes have become available, providing information on gene expression profiles for developmental stages and tissues of s. japonicum. here, we establish a public searchable database, termed sjtpd ... | 2008 | 18578888 |
| regulatory role of interleukin-10 and interferon-gamma in severe hepatic central and peripheral fibrosis in humans infected with schistosoma japonicum. | schistosoma japonicum is the most pathogenic agent of hepatosplenic schistosomiasis. it causes fibrosis of the central (centf) and peripheral (perf) portal areas. we investigated whether centf and perf in chinese fishermen infected with s. japonicum were associated with an abnormal production of cytokines and chemokines that, in experimental models, have been implicated in the regulation of fibrosis. | 2008 | 18582197 |
| hepatic ultrasound appearance and colonoscopic finding of schistosomiasis japonica. | 2008 | 18585968 | |
| evaluation of igg-elisa for the diagnosis of schistosoma japonicum in a high prevalence, low intensity endemic area of china. | antibody-based diagnostic methods for detecting infection with schistosoma japonicum have been developed and integrated into the national control program in china; however, the utility of these methods compared with conventional coprological methods remains unclear. in two consecutive years, we compared the performance characteristics of kato-katz with a commercially available enzyme-linked immunosorbent assay (elisa) that detects anti-egg antigen igg antibodies in a high prevalence, low intensi ... | 2008 | 18589392 |
| multiple near-identical genotypes of schistosoma japonicum can occur in snails and have implications for population-genetic analyses. | we genotyped (using 16 or 17 microsatellite loci) numerous adult schistosoma japonicum raised in rabbits exposed to pooled cercariae from small numbers of naturally infected snails from several localities in china. as expected, duplicate multi-locus genotypes (mlgs) were found among these worms. additionally, many more mlgs, often near-identical, were found than snails used as sources of cercariae. explanations for these results include (i) genotyping errors, (ii) development within each infecte ... | 2008 | 18590733 |
| retroposons of salmonoid fishes (actinopterygii: salmonoidei) and their evolution. | short and long retroposons, or non-ltr retrotransposons (sines and lines, respectively) are two groups of interspersed repetitive elements amplifying in the genome via rna and cdna-mediated reverse transcription. in this process, sines entirely depend on the enzymatic machinery of autonomous lines. the impact of retroposons on the host genome is difficult to overestimate: their sequences account for significant portion of the eukaryotic genome, while propagation of their active copies gradually ... | 2009 | 18590946 |
| molecular characterization of a calcium-binding protein sjca8 from schistosoma japonicum. | a full-length cdna encoding a cercarial stage-specifically expressed 8-kda calcium-binding protein (sjca8) was isolated from schistosoma japonicum cercarial cdna library using microarray screen. the putative gene coding for sjca8 is of 371 bp with an open reading frame of 69 amino acid (aa). the deduced aa sequence showed 83% identity with the schistosoma mansoni 8-kda cabp, 47% identity with clonorchis sinensis calcium-binding protein, and 38% identity with fasciola hepatica putative calcium-bi ... | 2008 | 18622627 |
| [effect of paeoniflorin on hepatic immunopathogenesis in mice with schistosoma japonicum infection]. | to investigate the mechanism of paeoniflorin in preventing hepatic granuloma formation and fibrosis in mice infected with schistosoma japonicum. | 2008 | 18637577 |
| [cloning, expression and identification of membrane protein tetraspanin 2-a of schistosoma japonicum]. | to clone and express a membrane protein (tetraspanin 2) gene of schistosoma japonicum (sjtsp2). | 2008 | 18637579 |
| [putation, identification and bioinformatics analysis of schistosoma japonicum aldehyde dehydrogenase full coding sequence]. | to acquire the full coding sequence of schistosoma japonicum aldehyde dehydrogenase, and fill the gaps of the partial aldehyde dehydrogenase sequences. | 2008 | 18637581 |
| [effect of igg3 antibody purified from sera of microtus fortis against schistosoma japonicum]. | igg3 antibody reaction to soluble antigens prepared from schistosomula (ssa), adult worms (sawa) and eggs (sea) in laboratory-bred microtus fortis (mf), balb/c mice and kunming (km) mice challenged by cercariae of schistosoma japonicum was detected by indirect elisa. the effect of purified igg3 antibody on in vitro killing schistosomula and protecting mice from infection of s. japonicum was evaluated. the igg3 antibody level in mf against ssa and sawa increased significantly by 79.6 percent and ... | 2008 | 18637582 |
| mri in cerebral schistosomiasis: characteristic nodular enhancement in 33 patients. | objective: the purpose of our study was to describe the characteristic mri appearance of cerebral infection with schistosoma japonicum. conclusion: cerebral infection with s. japonicum can cause a characteristic mri pattern of a large mass comprising multiple intensely enhancing nodules, sometimes with areas of linear enhancement. the typical appearance may be useful for diagnosis in endemic regions and may potentially be useful in cases imported into countries in which the disease is not endemi ... | 2008 | 18647936 |
| schistosoma japonicum infection modulates the development of allergen-induced airway inflammation in mice. | asthma, a chronic inflammatory disorder of the airways, is coordinated by th2 cells in both human asthmatics and animal models of allergic asthma. it has been shown that helminth infections including schistosoma mansoni may modulate atopic diseases including asthma. in the present study, balb/c mice were infected with bisexual and unisexual (male) s. japonicum, respectively, prior to ovalbumin (ova) sensitization and challenge. compared to mice with ova sensitization/challenge alone, s. japonicu ... | 2008 | 18654798 |
| a longitudinal study of comparison of the kato-katz technique and indirect hemagglutination assay (iha) for the detection of schistosomiasis japonica in china, 2001-2006. | from 2001 to 2006, about one-third of the residents aged 5-65 years were selected as the subjects in a schistosome-endemic village located in jiangxi province, china. every 1 year, all participants were analyzed by the kato-katz parasitologic examination and indirect hemagglutination assay (iha). statistical analysis of the results showed the k indices ranged from 0.250 to 0.347 between the two methods. assuming the kato-katz results as the gold standard reference, the specificity of the iha was ... | 2008 | 18675244 |
| remote sensing for predicting potential habitats of oncomelania hupensis in hongze, baima and gaoyou lakes in jiangsu province, china. | political and health sector reforms, along with demographic, environmental and socio-economic transformations in the face of global warming, could cause the re-emergence of schistosomiasis in areas where transmission has been successfully interrupted and its emergence in previously non-endemic areas in china. in the present study, we used geographic information systems and remote sensing techniques to predict potential habitats of oncomelania hupensis, the intermediate host snail of schistosoma ... | 2006 | 18686234 |
| strategy formulation for schistosomiasis japonica control in different environmental settings supported by spatial analysis: a case study from china. | with the aim of exploring the usefulness of spatial analysis in the formulation of a strategy for schistosomiasis japonica control in different environmental settings, a population-based database was established in dangtu county, china. this database, containing the human prevalence of schistosomiasis at the village level from 2001 to 2004, was analyzed by directional trend analysis supported with arcgis 9.0 to select the optimum predictive approach. based on the approach selected, different str ... | 2007 | 18686247 |
| the use of a vest equipped with a global positioning system to assess water-contact patterns associated with schistosomiasis. | the real exposure to many of the tropical diseases is difficult to assess at the individual-level due to problems of recall, self-reported diaries, personnel requirements, and altered behaviour related to observation. we present a study in an area endemic for schistosoma japonicum in which global positioning system (gps) receivers were used for personal time-activity monitoring to assess water-contact associated with schistosomiasis transmission. the study subjects were equipped with a vest with ... | 2007 | 18686248 |
| spatial risk profiling of schistosoma japonicum in eryuan county, yunnan province, china. | bayesian spatial risk profiling holds promise to enhance our understanding of the epidemiology of parasitic diseases, and to target interventions in a cost-effective manner. here, we present findings from a study using bayesian variogram models to map and predict the seroprevalence of schistosoma japonicum in eryuan county, yunnan province, china, including risk factor analysis. questionnaire and serological data were obtained through a cross-sectional survey carried out in 35 randomly selected ... | 2007 | 18686256 |
| spatio-temporal correlation between human and bovine schistosomiasis in china: insight from three national sampling surveys. | insight into the spatial and temporal contamination of the environment by bovine faeces in china can provide important information on the significance of bovines in the transmission of human schistosomiasis. this insight will be useful for the new evidence-based strategy of the chinese national schistosomiasis control programme. to enhance our understanding of the spatio-temporal relationship between the prevalence of human and bovine schistosomiasis, we performed correlation and regression anal ... | 2007 | 18686257 |
| the functional expression and characterisation of a cysteine peptidase from the invasive stage of the neuropathogenic schistosome trichobilharzia regenti. | a transcriptional product of a gene encoding cathepsin b-like peptidase in the bird schistosome trichobilharzia regenti was identified and cloned. the enzyme was named trcb2 due to its 77% sequence similarity to cathepsin b2 from the important human parasite schistosoma mansoni. the zymogen was expressed in the methylotropic yeast pichia pastoris; procathepsin b2 underwent self-processing in yeast media. the peptidolytic activity of the recombinant enzyme was characterised using synthetic fluoro ... | 2009 | 18708063 |
| [screening of t7 phage-display cdna library from liver of microtus fortis with extracts of schistosomulum and characterization of the positive clones]. | microtus fortis is naturally resisitent to schistosoma japonicum. in order to find schistosome-resistence-related genes of microtus fortis, a t7 phage-display cdna library from liver of microtus fortis was screened with the soluble lysate of schistosomula. the specific phages were enriched 375-fold after 3 rounds of biopanning. ninety-two positive clones picked at random were sequenced and 19 ests including 6 unreported genes were obtained. compared with the negative phage clone control, five po ... | 2008 | 18724690 |
| generalized negative binomial distribution: a promising statistical distribution for oncomelania hupensis in the lake- and marsh-land regions of china. | a new generalization of the negative binomial distribution (gnbd) is introduced and fitted to counts of oncomelania hupensis, the intermediate host of schistosoma japonicum, made, in areas of chinese lakeland and marshland, early in the winter of 2005 and late in the spring of 2006. the gnbd was found to fit the snail data better than the standard negative binomial distribution (nbd) that has previously been widely used to model the distribution of o. hupensis. with two more parameters than the ... | 2008 | 18782493 |
| three gorges dam and its impact on the potential transmission of schistosomiasis in regions along the yangtze river. | large-scale hydroprojects have a propensity for incurring schistosomiasis epidemics by altering the environments of their vicinities. as the construction of the three gorges dam, one of the world's largest hydroprojects to date, draws near its conclusion, an assessment of the dam's capacity in causing schistosomiasis becomes more urgent and pressing. this article reviews recent investigations into the possible effects of the dam on schistosomiasis in the three gorges region and areas along the y ... | 2008 | 18787917 |
| impact of changing water levels and weather on oncomelania hupensis hupensis populations, the snail host of schistosoma japonicum, downstream of the three gorges dam. | increasing evidence indicates that dams impact riverine ecosystems and human diseases. poyang lake, one of the largest schistosomiasis endemic environments in china, will change due to the construction of the yangtze river three gorges dam. we assess changes in oncomelania hupensis hupensis, the snail host for schistosoma japonicum, in response to changing water levels and weather from 1998 to 2002. in the 5 years following the major flooding of poyang lake in 1998, seasonal water levels have gr ... | 2008 | 18787918 |
| molecular cloning and expression of a functional anti-inflammatory protein, sj16, of schistosoma japonicum. | schistosomes are the causative agent of schistosomiasis. in the infected host, significant inflammatory response to the parasite is not observed. previous studies of schistosoma mansoni showed that this subdued inflammatory response was due to a 16-kda protein, sm16, which is present in high levels in the secretions of schistosomula. here we report the cloning and characterization of a gene (named sj16) from schistosoma japonicum. sequence analysis showed that sj16 shares 99% identity with sm16 ... | 2009 | 18804475 |
| the molluscan intermediate host and schistosomiasis japonica; observations on the production and rate of emergence of cercariae of schistosoma japonicum from the molluscan intermediate host, oncomelania quadrasi. | 1948 | 18889630 | |
| snail hosts of schistosoma japonicum in the soochow-wusih area, kiangsu, china. | 1948 | 18891634 | |
| an improved method of sedimenting schistosoma japonicum and other helminth ova. | 1947 | 18903604 | |
| experimental molluscan infection with schistosoma japonicum. | 1947 | 18921463 | |
| experimental mammalian infection with the schistosomes of man; comparative study of schistosoma mansoni and schistosoma japonicum infections produced by immersion and by intraperitoneal injection. | 1947 | 18921465 | |
| survival of schistosoma japonicum cercariae at various temperatures in several types of water. | 1947 | 18921467 | |
| the effects of sewage treatment processes on the ova and miracidia of schistosoma japonicum. | 1947 | 18921468 | |
| schistosomiasis control: experiences and lessons from china. | 2008 | 18930529 | |
| proteome approach for identification of schistosomiasis japonica vaccine candidate antigen. | experimental vaccination with radiation-attenuated cercariae (rac) confers possible practical levels of resistance to challenge infection by humoral and by cellular mechanism. here, we aimed to identify possible vaccine antigens by using specific igg antibody from rac vaccinated miniature pig. two milligrams of soluble egg antigen (sea) or schistosomal worm antigen preparation (swap) was fractionated using two dimensional liquid chromatography (proteome pf 2d) consisted of high performance chrom ... | 2009 | 18940265 |
| schistosoma japonicum antibody assay by immunosensing with fluorescence detection using 3,3',5,5'-tetramethylbenzidine as substrate. | an immunosensing system for schistosoma japonicum antibody (sjab) assay has been developed which is useful for the diagnosis of schistosomaisis. to circumvent the difficulty of regeneration of the immunosensing device, the sol-gel technique is used which results in a considerable retention of the activity of the encapsulated antigen (sjab) and easily diffusing into the pores of the polymeric silica matrix. the surface of the immunosensing device prepared can easily be renewed by simply polishing ... | 2002 | 18968789 |
| fluorometric enzyme immunosensing system based on a renewable immunoreaction platform for the detection of schistosoma japonicum antibody. | a fluoroimmunosensing device which was based on ferulic acid (fa)/horseradish peroxidase system for the detection of schistosoma japonicum antibody (sjab) has been developed. to circumvent the difficulty of regeneration of immunocomposite surface, a natural chitosan-epoxy resin matrix was used for the immobilization of sjag. the surface of the immunocomposite layer reacted was easily regenerated by simple polishing. the renewed surface served as a platform for the competitive immuno-reaction of ... | 2004 | 18969356 |
| expression and characterization of two tyrosinases from the trematode schistosoma japonicum. | tyrosinase (tyr) was thought to play a critical role during trematode egg production. in this study, we analyzed two genes (sjtyr1 and sjtyr2), derived from schistosoma japonicum genome databases, which encode proteins with significant homologies to mammalian tyr. they exhibited the typical tyr topology, including two copper-binding domains and a highly conserved cysteine-rich domain. semi-quantitative reverse transcription polymerase chain reaction showed that two sjtyr genes were mainly expres ... | 2009 | 18972131 |
| correlative and dynamic imaging of the hatching biology of schistosoma japonicum from eggs prepared by high pressure freezing. | schistosome eggs must traverse tissues of the intestine or bladder to escape the human host and further the life cycle. escape from host tissues is facilitated by secretion of immuno-reactive molecules by eggs and the formation of an intense strong granulomatous response by the host which acts to exclude the egg into gut or bladder lumens. schistosome eggs hatch on contact with freshwater, but the mechanisms of activation and hatching are poorly understood. in view of the lack of knowledge of th ... | 2008 | 19002240 |
| quantitative detection of schistosoma japonicum cercariae in water by real-time pcr. | in china alone, an estimated 30 million people are at risk of schistosomiasis, caused by the schistosoma japonicum parasite. disease has re-emerged in several regions that had previously attained transmission control, reinforcing the need for active surveillance. the environmental stage of the parasite is known to exhibit high spatial and temporal variability, and current detection techniques rely on a sentinel mouse method which has serious limitations in obtaining data in both time and space. ... | 2008 | 19015722 |
| expression profile, localization of an 8-kda calcium-binding protein from schistosoma japonicum (sjca8), and vaccine potential of recombinant sjca8 (rsjca8) against infections in mice. | researches on genes expressed in a cercarial stage-specific manner may help us understand the molecular events and functions during schistosome invasion of skin. a genomic clone encoding 8-kda calcium-binding protein (sjca8) specifically expressed in cercariae and skin-stage schistosomulum (transformed within 3 h) was obtained from cercariae. recombinant protein was expressed in vector pet32a (+) and purified using a ni-nta purification system. the target protein sjca8 was determined by matrix-a ... | 2009 | 19015878 |
| schistosoma japonicum: a pcr assay for the early detection and evaluation of treatment in a rabbit model. | a specific pcr assay for the detection of schistosoma japonicum dna in rabbit fecal and serum samples was developed by amplifying a 230-bp fragment from the sequence information of the clone g55a of the highly repetitive retrotransposon sjr2. the minimum amount of dna detectable using the pcr assay was 0.8pg, and the expected pcr product was amplified when dna equivalent of 1.1 egg from feces was used as template. in the meantime, serum anti-worm igg was examined by elisa. elisa gave positive re ... | 2009 | 19027005 |
| population genetics of schistosoma japonicum within the philippines suggest high levels of transmission between humans and dogs. | schistosoma japonicum, which remains a major public health problem in the philippines and mainland china, is the only schistosome species for which zoonotic transmission is considered important. while bovines are suspected as the main zoonotic reservoir in parts of china, the relative contributions of various non-human mammals to s. japonicum transmission in the philippines remain to be determined. we examined the population genetics of s. japonicum in the philippines in order to elucidate trans ... | 2008 | 19030225 |
| transport of glutathione transferase-fold structured proteins into living cells. | glutathione transferases are a family of enzymes that are traditionally known to contribute to the phase ii class of detoxification reactions. however, a novel property of the schistosoma japonicum glutathione transferase (sj.gst26) involves its translocation from the external medium into a variety of different cell types. here we explore the efficiency and mechanism of cell entry for this class of protein. using flow cytometry and confocal microscopy, we have examined the internalisation of sj. ... | 2009 | 19038230 |
| peroxiredoxin-1 from schistosoma japonicum functions as a scavenger against hydrogen peroxide but not nitric oxide. | three peroxiredoxins (prxs) are expressed during most of the developmental stages in the schistosome. prx-1 is localized on the surface of the schistosomula and adults of schistosoma japonicum, while prx-2 is localized in the sub-tegumental tissues, parenchyma, vitelline glands, and gut epithelium, but not on the surface of the worms. we applied rna interference techniques to suppress the specific genes of s. japonicum prxs. schistosomula of s. japonicum were cultured together with long-dsrna en ... | 2009 | 19041905 |
| diagnosis of schistosomiasis using recombinant fructose-1,6-bisphosphate aldolase from a formosan strain of schistosoma japonicum. | schistosoma japonicum obtained from taiwan is a zoophilic strain that only infects domestic and small animals. recombinant fructose-1,6-bisphosphate aldolase (fbpa) derived from this strain was used as an antigen in an enzyme-linked immunosorbent assay (elisa) for the diagnosis of human schistosomiasis. the full-length dna sequence of fbpa was found to be 1092 bp, encoding a protein of 363 amino acid residues, with a molecular mass of 39.6 kda. a total of 120 participants were recruited from chi ... | 2009 | 19079948 |
| identification and characterization of novel micrornas from schistosoma japonicum. | schistosomiasis japonica remains a major public health problem in china. its pathogen, schistosoma japonicum has a complex life cycle and a unique repertoire of genes expressed at different life cycle stages. exploring schistosome gene regulation will yield the best prospects for new drug targets and vaccine candidates. micrornas (mirnas) are a highly conserved class of noncoding rna that control many biological processes by sequence-specific inhibition of gene expression. although a large numbe ... | 2008 | 19107204 |
| transmission dynamics of schistosoma japonicum in the lakes and marshlands of china. | schistosoma japonicum is a major public health concern in china, with over one million people infected and another 40 million living in areas at risk of infection. unlike the disease caused by s. mansoni and s. haematobium, schistosomiasis japonica is a zoonosis, involving a number of different mammalian species as reservoir hosts. as a result of a number of published reports from china, it has long been considered that bovines, particularly water buffaloes, play a major role in human s. japonic ... | 2008 | 19115007 |
| [spatial distribution and elimination of schistosome japonicum intermediate hosts oncomelania hupensis in mountainous regions]. | to explore the spatial distribution and elimination of oncomelania hupensis in mountainous regions. | 2008 | 19115617 |
| [modeling transmission dynamics and control of schistosomiasis in the mountainous region, sichuan]. | to use a mathematical model and computer simulation to study transmission dynamics and control of schistosomiasis in mountainous regions of sichuan. | 2008 | 19115618 |
| mefloquine--an aminoalcohol with promising antischistosomal properties in mice. | the treatment and control of schistosomiasis, an often neglected tropical disease that exacerbates poverty, depends on a single drug, praziquantel. the large-scale use of praziquantel might select for drug-resistant parasites, hence there is a need to develop new antischistosomal compounds. here, we report that the antimalarial drug mefloquine possesses promising antischistosomal properties in mice. | 2009 | 19125172 |
| a strategy to control transmission of schistosoma japonicum in china. | schistosoma japonicum causes an infection involving humans, livestock, and snails and is a significant cause of morbidity in china. | 2009 | 19129526 |
| chronic schistosomiasis in a patient with rectal cancer. | 2009 | 19141828 | |
| [immuno-screening of schistosoma japonicum cercariae cdna library by the sera of anti-soluble cercariae 66 to approximately 68 kd antigens]. | to obtain the coding genes related to schistosoma japonicum (sj) cercariae 66 to approximately 68 kd antigens,and to provide antigens for diagnosis and vaccine of schistosomiasis. | 2008 | 19141971 |
| sensitivity of pooled serum testing for screening antibody of schistosomiasis japonica by iha in a mountainous area of yunnan, china. | pooled sample testing (pst) as a strategy for avoiding testing the majority of individual negative samples has been proposed for screening of diseases in low prevalence areas. there has been no standard guideline for pst in screening of schistosoma japonicum infection of yunnan, china. to document the optimum pool size with acceptable sensitivity of pst for screening of schistosoma japonicum infection in this setting, an experimental pooling of each of 31 positive sera by iha with various number ... | 2009 | 19154655 |
| [morphological observation on the reproductive system of adult schistosoma japonicum under confocal laser scanning microscopy]. | balb/c mice were infected with schistosoma japonicum cercariae (40+/-2 per mouse) through abdominal skin. mice were sacrificed after 35 days to acquire the adult worms which were then fixed, stained, clarified, dehydrated and mounted. the specimens were observed under the confocal laser scanning microscope. the overall morphology of the adult worms was displayed distinctly, especially the testicular lobes, seminal vesicle and genital pore of the male, reproductive system, and the ovary, vitellin ... | 2008 | 19157309 |
| [cloning, expressing and characterizing of a phosphoglycerate mutase gene of schistosoma japonncum]. | phosphoglycerate mutase (pgam) is a key enzyme in glycolytic pathways. with pcr technique based on an est identified in our lab, a novel gene named sjpgam (genbank accession no. eu374631) was cloned. sequence analysis revealed that the orf of sjpgam gene contained 753 nucleotides, encoding 250 amino acids, and the molecular weight was about 28.26 kd. real-time pcr analysis showed that the mrna level of sjpgam was much higher in the 14 days and 19 days schistosomula than other stages, suggesting ... | 2008 | 19160836 |
| [gene synthesis, expression and immunogenicity analysis of tsp2 hydrophilic domain(tsp2hd) of schistosoma japonicum]. | to synthesize and express the gene of tsp2 hydrophilic domain of schistosoma japonicum, and investigate the immunogenicity of the recombinant tsp2hd protein. | 2008 | 19160958 |
| [suppressive effect of induced cd4+cd25+ regulatory t cells from mice infected with schistosoma japonicum]. | to investigate the suppressive effect of cd4+cd25+ regulatory t cells from mice infected with schistosoma japonicum. | 2008 | 19160959 |
| [up-expression of pd-1-pd-l induced by immunization with sea and smwa of schistosoma japonicum in mice]. | to explore the expression of pd-1-pd-l pathway of mice immunized with soluble egg antigen (sea) or soluble male worm antigen (smwa) of schistosoma japonicum. | 2008 | 19160960 |
| impact of the south-to-north water diversion project on the transmission of schistosoma japonicum in china. | the south-to-north water diversion project (snwdp) is currently the key, national, water-conservation project in china, designed to optimise the use of water resources and relieve the water shortages in the north of the country. as one of the main water intakes for the project, that of the eastern route scheme (ers), is a breeding site for oncomelania hupensis (the intermediate host of schistosoma japonicum), there is concern that the snail may be carried far to the north, in the water passing t ... | 2009 | 19173773 |
| using affinity chromatography to engineer and characterize ph-dependent protein switches. | conformational changes play important roles in the regulation of many enzymatic reactions. specific motions of side chains, secondary structures, or entire protein domains facilitate the precise control of substrate selection, binding, and catalysis. likewise, the engineering of allostery into proteins is envisioned to enable unprecedented control of chemical reactions and molecular assembly processes. we here study the structural effects of engineered ionizable residues in the core of the gluta ... | 2009 | 19177365 |
| histopathological changes in adult schistosoma japonicum harbored in mice treated with a single dose of mefloquine. | new research has shown that mefloquine, an arylaminoalcohol used against malaria, is active against schistosoma japonicum and schistosoma mansoni in vivo. to enhance our understanding of the potential mechanism of action of mefloquine against schistosomiasis, we examined the dynamics of histopathological changes in adult s. japonicum. mice infected with s. japonicum for 35 days were treated intragastrically with a single dose of mefloquine (400 mg/kg). one to 35 days after mefloquine administrat ... | 2009 | 19221797 |
| low sensitivity of the formol-ethyl acetate sedimentation concentration technique in low-intensity schistosoma japonicum infections. | the endemic countries are in a diagnostic dilemma concerning schistosoma japonicum with increasing difficulties in diagnosing the infected individuals. the formol-ethyl acetate sedimentation concentration technique is preferred by many clinical microbiology laboratories for the detection of parasites in stool samples. it is potentially more sensitive than the diagnostic methods traditionally used. | 2009 | 19238192 |
| [researches on cloning and expression of the gene encoding schistosoma japonicum tetraspanins extracellular loop 2 and its immunogenicity]. | to clone and express the gene (sj-tsp-2) encoding extracellular loop 2 (ec-2) of tetraspanins (tsps) of schistosoma japonicum (chinese strain), and then to study the antigenicity and immunogenicity of this protein. | 2008 | 19253819 |
| finding genes in schistosoma japonicum: annotating novel genomes with help of extrinsic evidence. | we have developed a novel method for estimating the parameters of hidden markov models for gene finding in newly sequenced species. our approach does not rely on curated training data sets, but instead uses extrinsic evidence (including paired-end ditags that have not been used in gene finding previously) and iterative training. this new method is particularly suitable for annotation of species with large evolutionary distance to the closest annotated species. we have used our approach to produc ... | 2009 | 19264800 |
| immunoglobulin e (ige) responses to paramyosin predict resistance to reinfection with schistosoma japonicum and are attenuated by igg4. | schistosomiasis remains a public health concern in developing countries, and rapid reinfection fostered by continued exposure to contaminated water sources necessitates a vaccine to augment current mass treatment-based control strategies. we report isotype-specific (immunoglobulin a [iga], ige, igg1, igg4, and igg) antibody responses to soluble worm antigen preparation and the recombinant vaccine candidates rsj97, rsj67, and rsj22 from a schistosoma japonicum-infected cohort in leyte, the philip ... | 2009 | 19273558 |
| in vitro and in vivo evaluation of small interference rna-mediated gynaecophoral canal protein silencing in schistosoma japonicum. | schistosomiasis causes liver and intestinal damage and can be very debilitating. the pairing of a male worm with a female worm residing in the gynaecophoral canal of male plays a critical role in the development of female parasite. because the male specific gynaecophoral canal protein of schistosoma japonicum (sjgcp) is found in significant quantities in the adult female worm after pairing, it could play an important role in parasite pairing. | 2009 | 19288459 |
| [a multi-gene dna vaccine encoding non-fusion membrane-anchoring antigen against schistosoma japonicum]. | to construct dna vaccine (pires-sj97-sj14-sj26) and study its immunogenicity and protective immunity against schistosoma japonicum. | 2008 | 19288912 |
| [suppression effect of different stage antigens of schistosoma japonicum on airway inflammation in a murine model of asthma]. | to investigate the effect of antigens of different stage schistosoma japonicum on airway inflammation in a murine model of asthma. | 2008 | 19288916 |
| [anti-fecundity effect of soluble immature egg antigen of schistosoma japonicum in mice]. | to study anti-female fecundity effect in mice immunized with soluble immature egg antigen (siea) of schistosoma japonicum, and observe possible mechanism. | 2008 | 19288926 |
| modeling the dynamics and control of transmission of schistosoma japonicum and s. mekongi in southeast asia. | a mathematical model for transmission of schistosomes is useful to predict effects of various control measures on suppression of these parasites. this review focuses on epidemiological and environmental factors in schistosoma japonicum and schistosoma mekongi infections and recent advances in mathematical models of schistosoma transmission. | 2009 | 19290084 |
| cerebellar schistosomiasis: a case report with clinical analysis. | the authors report here a rare case of cerebellar schistosomiasis identified by pathological diagnosis, lacking extracranial involvement. the clinical symptoms included headache, dizziness, and nausea. studies in blood were normal and no parasite eggs were detected in stool. computed tomography of brains showed hypodense signal, and magnetic resonance imaging showed isointense signal on t1-weighted images, hyperintense signal on t2-weighted images, and intensely enhancing nodules in the right ce ... | 2009 | 19290092 |
| schistosoma japonicum: proteomics analysis of differentially expressed proteins from ultraviolet-attenuated cercariae compared to normal cercariae. | schistosomiasis is considered the most important human helminthiasis in terms of morbidity and mortality. in this study, comparative soluble proteomic analysis of normal cercariae and ultraviolet-irradiated attenuated cercariae (uvac) from schistosoma japonicum were carried out in view of the high efficiency of irradiation-attenuated cercariae vaccine. the results revealed that some proteins showed significant differential expression in the parasite after treatment with ultraviolet light. total ... | 2009 | 19290541 |
| leveraging rural energy investment for parasitic disease control: schistosome ova inactivation and energy co-benefits of anaerobic digesters in rural china. | cooking and heating remain the most energy intensive activities among the world's poor, and thus improved access to clean energies for these tasks has been highlighted as a key requirement of attaining the major objectives of the un millennium development goals. a move towards clean energy technologies such as biogas systems (which produce methane from human and animal waste) has the potential to provide immediate benefits for the control of neglected tropical diseases. here, an assessment of th ... | 2009 | 19293926 |
| excretory/secretory proteome of the adult developmental stage of human blood fluke, schistosoma japonicum. | schistosomes are the causative agents of schistosomiasis, one of the most prevalent and serious of the parasitic diseases that currently infects approximately 200 million people worldwide. schistosome excretory/secretory (es) proteins have been shown to play important roles in modulating mammalian host immune systems. in our current study, we performed a global proteomics identification of the es proteins from adult worms of schistosoma japonicum, one of the three major schistosome species. our ... | 2009 | 19299421 |
| genetic variability among schistosoma japonicum isolates from different endemic regions in china revealed by sequences of three mitochondrial dna genes. | the present study examined sequence variation in three mitochondrial dna (mtdna) regions, namely cytochrome c oxidase subunit 3 (cox3), nadh dehydrogenase subunits 4 and 5 (nad4 and nad5), among schistosoma japonicum isolates from different endemic regions in china, and their phylogenetic relationships were re-constructed. a portion of the cox3 gene (pcox3), a portion of the nad4 and nad5 genes (pnad4 and pnad5) were amplified separately from individual trematodes by polymerase chain reaction (p ... | 2009 | 19303214 |
| disseminated peritoneal schistosoma japonicum: a case report and review of the pathological manifestations of the helminth. | schistosomiasis (also known as bilharzia, bilharziasis, bilharziosis or snail fever) is a human disease syndrome caused by infection from one of several species of parasitic trematodes of the genus schistosoma. the three main species infecting humans are s haematobium, s japonicum, and s mansoni. s japonicum is most common in the far east, mostly in china and the philippines. we present an unusual case of s japonicum in a 32-year-old filipino woman who had schistosomal ova studding the peritonea ... | 2009 | 19318750 |
| developmental gene expression profiles of the human pathogen schistosoma japonicum. | the schistosome blood flukes are complex trematodes and cause a chronic parasitic disease of significant public health importance worldwide, schistosomiasis. their life cycle is characterised by distinct parasitic and free-living phases involving mammalian and snail hosts and freshwater. microarray analysis was used to profile developmental gene expression in the asian species, schistosoma japonicum. total rnas were isolated from the three distinct environmental phases of the lifecycle -- aquati ... | 2009 | 19320991 |
| imaging schistosomes in vivo. | schistosomes are intravascular, parasitic helminths that cause a chronic, often debilitating disease afflicting over 200 million people in over 70 countries. here we describe novel imaging methods that, for the first time, permit visualization of live schistosomes within their living hosts. the technology centers on fluorescent agent uptake and activation in the parasite's gut, and subsequent detection and signal quantitation using fluorescence molecular tomography (fmt). there is a strong posit ... | 2009 | 19346298 |
| separation of hexahistidine fusion proteins with immobilized metal ion affinity chromatographic (imac) sorbents derived from m(n+)-tacn and its derivatives. | the capabilities of a new class of immobilized (im) metal ion chelate complexes (imccs), derived from 1,4,7-triazacyclononane (tacn), bis(1,4,7-triazacyclononyl) ethane (dtne) and bis(1,4,7-triazacyclononyl)propane (dtnp) complexed with the borderline metal ions cu(2+), ni(2+), zn(2+), mn(2+), co(2+), and cr(3+), for the purification of proteins have been investigated. in particular, the binding behavior of a model protein, the c-terminal hexahistidine tagged recombinant fusion protein schistoso ... | 2009 | 19350626 |
| parasite genetic differentiation by habitat type and host species: molecular epidemiology of schistosoma japonicum in hilly and marshland areas of anhui province, china. | schistosoma japonicum, a parasite of significant public health importance in parts of china and southeast asia, is a true generalist pathogen with over 40 species of mammals suspected as definitive host reservoirs. in order to characterize levels of parasite gene flow across host species and identify the most important zoonotic reservoirs, s. japonicum larvae (miracidia) were sampled from a range of definitive host species in two contrasting habitat types within anhui province, china: a low-lyin ... | 2009 | 19389178 |
| a multi-level analysis of risk factors for schistosoma japonicum infection in china. | the aim of this study was to explore the risk factors of schistosomiasis japonica in china, using a hierarchical multi-level model with individuals nested within villages. | 2009 | 19398361 |
| endocannabinoids anandamide and its cannabinoid receptors in liver fibrosis after murine schistosomiasis. | this study examined endogenous cannabinoid (ecb)-anandamide (aea) and its cannabinoid receptors (cbr) in mice liver with the development of schistosoma japonicum. mice were infected with schistosoma by means of pasting the cercaria onto their abdomens. liver fibrosis was pathologically confirmed nine weeks after the infection. high performance liquid chromatography (hplc) was employed to determine the concentration of aea in the plasma of mice. immunofluorescence was used to detect the expressio ... | 2009 | 19399401 |
| conquering 'snail fever': schistosomiasis and its control in china. | schistosomiasis japonica is a serious parasitic disease and a major health risk for more than 60 million people living in the tropical and subtropical zones of south china. the disease is a zoonosis and its cause, the parasitic trematode schistosoma japonicum, has a range of mammalian reservoirs, making control efforts difficult. current control programs are heavily based on community chemotherapy with a single dose of the highly effective drug praziquantel. however, vaccines (for use in bovines ... | 2009 | 19400766 |
| th1-type epitopes-based cocktail pddv attenuates hepatic fibrosis in c57bl/6 mice with chronic schistosoma japonicum infection. | schistosomiasis is one of the world's major public health problems in terms of morbidity and mortality, which is characterized by a marked egg-induced cd4(+) t-cell programmed granulomatous inflammation and cumulative fibrosis. here pddv (peptide-dna dual vaccine), a widely used non-viral gene delivery system, was applied. the cocktail pddv, based on four th1-type epitope peptides identified from schistosoma japonicum vaccine candidates and cpg odn1826, could induce dominant th1-type response in ... | 2009 | 19410625 |
| location of active transmission sites of schistosoma japonicum in lake and marshland regions in china. | schistosomiasis control in china has, in general, been very successful during the past several decades. however, the rebounding of the epidemic situation in some areas in recent years raises concerns about a sustainable control strategy of which locating active transmission sites (ats) is a necessary first step. this study presents a systematic approach for locating schistosomiasis ats by combining the approaches of identifying high risk regions for schisotosmiasis and extracting snail habitats. ... | 2009 | 19416552 |
| a schistosoma japonicum chimeric protein with a novel adjuvant induced a polarized th1 immune response and protection against liver egg burdens. | schitosomiasis japonica is still a significant public health problem in china. a protective vaccine for human or animal use represents an important strategy for long-term control of this disease. due to the complex life cycle of schistosomes, different vaccine design approaches may be necessary, including polyvalent subunit vaccines. in this study, we constructed four chimeric proteins (designated sjgp-1~4) via fusion of sj26gst and four individual paramyosin fragments. we tested these four prot ... | 2009 | 19419545 |
| detection of circulating antigen in serum of mice infected with schistosoma japonicum by immunomagnetic bead elisa based on igy. | we developed a novel immunomagnetic bead elisa based on igy (egg yolk immunoglobulin) for detection of circulating antigen (ca) in sera of mice infected with schistosoma japonicum. the assay involved the use of chicken polyclonal antibodies igy against soluble egg antigens (sea) of s. japonicum as a capture antibody and anti-sea mouse monoclonal antibody np28-5b labeled horseradish peroxidase (hrp) as a detecting antibody. two groups of balb/c mice infected with s. japonicum cercariae were used: ... | 2009 | 19426661 |
| analysis of early hepatic stage schistosomula gene expression by subtractive expressed sequence tags library. | schistosome parasites require a complex lifecycle requiring two hosts and aquatic phases of development. the schistosomula is a key phase of parasite development within the mammalian host, however relatively little is understood about the molecular processes underlying this stage. in this study 5723 subtractive expressed sequence tags (ests) were randomly selected from a 7 day hepatic schistosomula enriched library constructed using suppression subtractive hybridization method. sequence analysis ... | 2009 | 19428674 |
| landscape genetics: the correlation of spatial and genetic distances of oncomelania hupensis, the intermediate host snail of schistosoma japonicum in mainland china. | two internal transcribed spacer (its1 and its2) sequences of the non-coding region of the rdna and a 16s rna gene fragment of the mtdna of oncomelania hupensis, the intermediate host snail of schistosoma japonicum in mainland china, have been investigated with a view to illustrate the influence of the environment on genetic differentiation. thirteen populations of o. hupensis snails, representing four types of ecological settings, were collected to compare genetic and spatial distances. the leng ... | 2009 | 19440964 |
| the etiology of liver damage imparts cytokines transforming growth factor beta1 or interleukin-13 as driving forces in fibrogenesis. | it is unknown whether transforming growth factor beta1 (tgf-beta1) signaling uniformly participates in fibrogenic chronic liver diseases, irrespective of the underlying origin, or if other cytokines such as interleukin (il)-13 share in fibrogenesis (e.g., due to regulatory effects on type i pro-collagen expression). tgf-beta1 signaling events were scored in 396 liver tissue samples from patients with diverse chronic liver diseases, including hepatitis b virus (hbv), hepatitis c virus (hcv), schi ... | 2009 | 19441105 |
| effect of single-dose oral mefloquine on the morphology of adult schistosoma japonicum in mice. | it has been recently documented that the antimalarial drug mefloquine shows in vivo activity against schistosomes. in the present study, we assessed the effect of mefloquine on the morphology of adult schistosoma japonicum worms. mice were infected with s. japonicum cercariae for 35 days and then treated with a single 400-mg/kg oral dose of mefloquine. groups of mice were killed between 24 h and 14 days post-treatment and worms were recovered from the liver and mesenteric veins, fixed in 70% alc ... | 2009 | 19458964 |
| [immunological identification of a synthetic peptide from the paramyosin of schistosoma japonicum]. | to identify the immunologic property of a synthetic peptide sj97-p22 from paramyosin of schistosoma japonicum (sj97). | 2009 | 19459496 |
| [immunoscreening and identification of schistosoma japonicum juvenile cdna library]. | the cdna library of schistosoma japonicum (sj) juveniles was immunoscreened with the anti-serum from day 14 post-infection mice. the inserts of the seven positive clones were sequenced and analyzed for their homology in genbank database. results showed that one was highly homologous to the sjhsp70 (score=650), two were significantly homologous to the sjfabp (score=229) and sj cdgsh-type zn finger-containing protein-like protein (score=246), and the other four were not homologous to genes in genb ... | 2009 | 19459509 |
| rna interference targeting leucine aminopeptidase blocks hatching of schistosoma mansoni eggs. | schistosoma mansoni leucine aminopeptidase (lap) is thought to play a central role in hatching of the miracidium from the schistosome egg. we identified two discrete laps genes in the s. mansoni genome, and their orthologs in s. japonicum. the similarities in sequence and exon/intron structure of the two genes, lap1 and lap2, suggest that they arose by gene duplication and that this occurred before separation of the mansoni and japonicum lineages. the smlap1 and smlap2 genes have different expre ... | 2009 | 19463860 |
| il-18 enhances protective effect in mice immunized with a schistosoma japonicum fabp dna vaccine. | two recombinant plasmids, pvax/sjfabp and pvax/mil-18 containing schistosoma japonicum 14 kda fatty acid binding protein (sjfabp) and murine il-18, were constructed and evaluated for their ability to induce immune responses and to protect against s. japonicum challenge in mice. mice were intramuscularly immunized twice at three-weekly intervals, and challenged with s. japonicum cercariae at 4 weeks after the last vaccination. all animals vaccinated with pvax/sjfabp alone or plus pvax/mil-18 deve ... | 2009 | 19467215 |
| familial aggregation of human helminth infection in the poyang lake area of china with a focus on genetic susceptibility to schistosomiasis japonica and associated markers of disease. | human helminthiases are common in china, especially in rural areas where sanitation conditions are poor. soil-transmitted helminths (sths) are predominantly found in the southern provinces. schistosoma japonicum is also endemic to southern china. here we review the prevalence of helminth infections and polyparasitism in china, and discuss the interactions between helminth parasites in the co-infected host. it is clear that sths are more prevalent in rural china than previously suggested emphasiz ... | 2009 | 19486544 |
| smcl3, a gastrodermal cysteine protease of the human blood fluke schistosoma mansoni. | blood flukes of the genus schistosoma are platyhelminth parasites that infect 200 million people worldwide. digestion of nutrients from the host bloodstream is essential for parasite development and reproduction. a network of proteolytic enzymes (proteases) facilitates hydrolysis of host hemoglobin and serum proteins. | 2009 | 19488406 |
| schistosomiasis in china: acute infections during 2005-2008. | significant progress has taken place over the past 50 years in the control of schistosomiasis japonica in china. however, the available data suggested that schistosomiasis has re-emerged shortly after the world bank loan project which was conducted from 1992 to 2001. the national control program with a revised strategy to control schistosomiasis by using integrated measures has been implemented since 2005. in this study, we aimed to evaluate the effect of the national program on schistosomiasis ... | 2009 | 19493433 |