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life cycle dependent phototactic orientation in ophryoglena catenula.the histophagous ciliate ophryoglena catenula, which is characterized by a conspicuous "watchglass organelle", shows a pronounced orientation with respect to the direction of light. the five stages of the common reproduction cycle are all light sensitive: positive phototaxis was regularly found in "theronts" exposed to unilateral white light at intermediate fluence rates (e.g. 10 klx), and also in "trophonts" and "protomonts" within 2.5 hours after feeding. negative phototaxis was observed under ...201123195647
ultrastructure of the rumen ciliate dasytricha ruminantium.the ciliate dasytricha ruminantium possesses cilia over the entire surface. both the oral and somatic kineties are composed of monokinetids comprising a kinetosome, a tangential ribbon of 5 transverse microtubules, a weakly convergent bundle of 5 postciliary microtubules, a short kinetodesmal fibre, and a single microtubule homologous to t(2) of other litostomate ciliates [15]. the reversed orientation of the kineties within and around the vestibulum may be a consequence of the evolutionary migr ...201123195656
morphogenesis and ultrastructure of the soil ciliate engelmanniella mobilis (ciliophora, hypotrichida).the morphogenetic pattern, freeze-fracture, transmission, and scanning electron-microscopy of interphasic cells were used to elucidate the enigmatic systematic position of engelmanniella mobilis. frontal, buccal, parabuccal, and marginal cirri are distinguishable during cortical development; transverse, frontoterminal (migratory), and caudal cirri are absent. ontogenetic peculiarities include the conservation of parental and grandparental marginal cirri and the apokinetal origin of a dorsal kine ...201123195727
prorodon spirogyrophagus nov. spec. (ciliophora, prostomatea): morphology, infraciliature and food-uptake.prorodon spirogyrophagus nov. spec. is described by observations on living and silver-stained specimens. important morphometric data are presented and the feeding habits are described. the new species is characterized by a body size of about 450 × 200 μm, numerous independently functioning contractile vacuoles each with an excretion porus, long caudal cilia, about 230 longitudinal somatic kineties, and three brush kineties. its life cycle includes division, digestive, and resting cysts. the new ...201123195730
morphogenesis of bipartition in two species of the genus ptychostomum (ciliophora, hysterocinetidae).two new species of hysterocinetidae ciliates living parasitically in the alimentary tract of alma nilotica are described along with the process of stomatogenesis in these species. the buccal primordium of the opisthe originates from the multiplication of kinetosomes of some somatic kineties forming a disorganized field. one row of kinetosomes, a second and a third are successively isolated from this field. as soon as this stage with three rows of kinetosomes is reached, the peristomial infracili ...201123195738
ultrastructural study of euplotidium itoi (ciliata hypotrichida).details of the cortical, cytoplasmic, and nuclear ultrastructure of euplotidium itoi are provided here for the first time. when these ultrastructural characteristics are compared with the corresponding features of representative members of the family euplotidae, it arises that the differences between e. itoi and these individual taxa fall into the range of variability of the family. as reported elsewhere [29] e. itoi harbors peculiar epibionts (epixenosomes) inserted in a sort of band running al ...201123195744
north american and eurasian strains of stylonychia lemnae (ciliophora, hypotrichida) have a high genetic identity, but differ in the nuclear apparatus and in their mating behavior.it was investigated whether the closely related species stylonychia lemnae and stylonychia mytilus occur in north america. eigthy-one stylonychia cells were collected in the surroundings of ithaca, n.y., usa. a comparison of their isoenzyme patterns and the number of dorsal cilia with those of eurasian clones demonstrated that 79 clones belong to s. lemnae and 2 to s. mytilus. the mean genetic identity between the european and the north american populations of s. lemnae is 84% which is character ...201123195788
purification of the surface membrane-cytoskeleton complex (cortex) of paramecium and identification of several of its protein constituents.in ciliates, the major morphogenetic events take place in the cortex, a complex of membranes and closely associated filamentous networks. to analyze the problems of assembly and morphogenesis at the molecular level in paramecium, we have developed a method of purification of cortex fragments which retain their in situ organization and display a highly reproducible electrophoretic profile. the method used either a four-step sucrose gradient yielding a cortex + oral apparatus fraction or a six-ste ...201123195967
relations between growth rate, cell size, and dna content in colpodean ciliates (ciliophora: colpodea).optimal conditions for growth of 9 strains from 7 species of colpodean ciliates were determined using a factorial experimental design. estimates of maximal growth rate were higher than those previously obtained for similar-sized ciliates. using published cell size estimates, the slope of a cell size-growth rate relation was found to be not significantly different from zero, either when maximal growth rates were used or when all growth rates were scaled to 20°c, a suboptimal temperature. both mac ...201123196048
morphology and morphogenesis of disematostoma colpidioides (ciliophora, frontoniidae): its systematic implications.disematostoma colpidioides, until now, wenrichia colpidioides, is a reniform ciliate with a strong torsion at the equatorial plane. the nuclear apparatus consists of a long macronucleus and a spherical micronucleus. the oral infraciliature is made up by a paroral kinety and three peniculi with 4,4, and 3 rows of kinetosomes, respectively. at the right slope of the buccal cavity there are 3-5 vestibular kineties. the somatic infraciliature consists of dikinetids which form a preoral and a postora ...201123196049
use of nile red as a rapid measure of lipid content in ciliates.we report the use of nile red as a rapid and inexpensive method to estimate cellular lipids in three species of paramecium and in tetrahymena by the direct application of the dye to living or fixed cells without extraction and purification. qualitative estimates of the relative changes in the lipid content of cells of varying culture ages were obtained using fluorescence microscopy, while semiquantitative determinations were obtained by measuring the total fluorescence from the emission spectrum ...201123196050
[in process citation].during four different time periods artificial substrates (polyuréthane blocs) were used to sample ciliates in a river receiving different kinds of pollution. 46 species of ciliates were found, belonging to 40 genera. their distribution and abundance varied greatly both along the river and with time. the saprobic index method was used to localise sources of organic inputs and follow their change with time. community structures were analysed through specific diversities and rank-frequency diagrams ...201123196052
recent advances in the study of ciliate genes.this review gives a brief report on some recent advances in the study of molecular biology of ciliate genes. the main emphasis will be put on those results which may contribute to a better understanding of how gene structure and genome organization has evolved in ciliates. new data will be discussed in relation to the contribution they might give for a general understanding of eukaryotic gene evolution. the whole topic is divided into four parts: (1) a short introduction into genome organization ...201123196120
description of two species of nassophorean ("hypostome") ciliates, nassulopsis elegans (ehrenberg, 1833) and zosterodasys derouxi n.sp., with comments on their systematic and phylogenetic affinities.most of the so-called "lower hypostomes", nassophorean ciliates in the most recent classifications of the phylum ciliophora, have been little studied in modern times (e.g., employing methods of silver impregnation, a technique widely considered indispensable in comparative taxonomic work on these protists today). in this paper, we present descriptions of two species, a new strain of nassulopsis elegans (ehr., 1833) and zosterodasys derouxi n.sp., based primarily on use of the pyridinated silver ...201123196121
conjugation between unimicronuclear doublets and singlets, and several features of the clones originating from hemikarya of euplotes woodruffi syngen 1 (ciliophora).conjugation of a unimicronuclear doublet with two singlets produced three kinds of exconjugants: an exconjugant singlet cell, with a macronucleus and a micronucleus, originating from a synkaryon (diploid); an exconjugant singlet with a micronucleus and a macronucleus from a hemikaryon (haploid); and an exconjugant doublet consisting of two components, one side bearing a micronucleus and a macronucleus derived from a hemikaryon and the other side with those from a synkaryon. of the two exconjugan ...201123196125
ultrastructural localization of chitin in the cystic wall of euplotes muscicola kahl (ciliata, hypotrichia).the ultrastructural localization of chitin in the cyst walls of euplotes muscicola was carried out after deproteinization and by specific labelling with wga-gold complex. the overall ectocyst contains chitin that alone can sustain the exact profile of this cystic layer. in the endocyst, electron clear sublayers include chitin and alternate with other sublayers probably of proteic nature.201123196126
the oral apparatus of colpoda variabilis (ciliophora, colpodidae): i. 3-d reconstruction by serial semi-thin sections and low temperature scanning electron microscopy.a three-dimensional reconstruction of the oral apparatus of colpoda variabilis was done by serial semi-thin sections and low temperature scanning electron microscopy. the inner part of the oral cavity is separated from the outer part by an oral ridge on its left side and by a vestibular ridge on its right side. a banana-shaped left oral polykinetid is situated on the left wall of the oral cavity, not extending into the inner part of the oral cavity. a triangular-shaped right oral polykinetid is ...201123196127
autoecological and molecular approach to the species problem in the euplotes vannus-crassus-minuta group (ciliophora, hypotrichida).the long standing species problem in the euplotes vannus-crassus-minuta group is addressed here by means of restriction enzyme rdna maps and salinity and temperature tolerance tests performed on several strains of these morphospecies (i.e. morphologically identified species). the rationale in collecting such data (which add to others existing in literature) is to provide a broad data base to allow a multi-integrated approach to the problem at hand. the autoecological parameters considered and th ...201123196188
infraciliature and silverline system of the ciliate pseudotrachelocerca trepida (kahl, 1928), nov. gen., nov. comb., and establishment of a new family, the pseudotrachelocercidae nov. fam. (ciliophora, haptoria).the morphology, infraciliature and silverline system of trachelocerca trepida kahl, 1928 has been reinvestigated. the species was found in marine water (yellow sea) near shanghai, eastchina. stained specimens (protargol) are characterized morphometrically. the shape of the extrusomes (toxicysts) and the structure of oral as well as somatic kinetids indicate that this species must be separated from the genus trachelocerca. a new genus, pseudotrachelocerca, characterized by an extensible neck, spi ...201123196190
ultrastructure and morphogenesis of the marine epibenthic ciliate epiclintes ambiguus (epiclintidae, n. fam.; ciliophora).epiclintes ambiguus, a highly contractile marine ciliate, has ventral cirri in oblique and marginal rows, and three rows of dorsal cilia, thus is at the hypotrich structural grade. we use light microscopy of living and protargol stained specimens and both scanning and transmission electron microscopy to describe cortical morphogenesis through cell division and interphase cortical structure of e. ambiguus. cortical morphogenesis proceeds in 2 oblique developmental zones and is based on 4 kinds of ...201123196193
the biology of free-living anaerobic ciliates.anaerobic ciliates are incapable of using oxidative phosphorylation in their energy metabolism and they are more or less sensitive to oxygen. all anaerobic ciliates possess mitochondria-like organelles (with a double outer membrane and often a few cristae) but these do not contain typical mitochondrial enzymes (e.g., cytochromes, cytochrome oxidase). in some species these organelles are capable of fermenting pyruvate into acetate and h2 and they are then referred to as hydrogenosomes. at least s ...201123196279
free-living ciliates from the coastal area of lake tanganyika (africa).lake tanganyika is world famous for a fauna that is rich in endemic species. on the other hand, it seems to be particularly poor in species of planktonic ciliates. only the coastal infusorial fauna, for the most part mesopsammic, constitutes an extensive biomass with relatively few species. however, some are characteristic (planicoleps psammophilus n.g., n.sp. frontonia tanganyikae n.sp.). in these cases, one can speak of endemism.201123196280
entodiniomorphid ciliates from the wild lowland gorilla with the description of a new genus and three new species.the entodiniomorphid ciliates in gorilla in gabon, west africa, were surveyed and observed by light and scanning electron microscopy. as a result, 4 species belonging to the families troglodytellidae and cycloposthiidae were identified, and 3 of them were new to science. these species were described as goriliophilus thoracatus n.g., n.sp., troglodytella gabonensis n.sp., and prototapirella gorillae n.sp. sem observations of goriliophilus and troglodytella revealed in particular the surface struc ...201123196284
the oral apparatus of colpoda variabilis (ciliophora, colpodidae): ii. ultrastructure of the oral ciliature and its implications on ciliate phylogeny.the oral ciliature of colpoda variabilis has been examined by serial thin sections. the left oral polykinetid consists of parallel rows of ciliated kinetosomes. the right oral polykinetid consists of a ventral part of ciliated kinetosomes in anarchic arrangement and a dorsal double row of ciliated kinetosomes. the existence of postciliary microtubules in association with each kinetosome makes it possible to determine the orientation of kinetosomes within the oral polykinetids. the orientation of ...201123196286
ultrastructure of the mycophagous ciliate grossglockneria acuta (ciliophora, colpodea) and phylogenetic affinities of colpodid ciliates.ultrastructural features of grossglockneria acutafoissner, 1980 are very similar to those known in the confamilial species, pseudoplatyophrya nana. the somatic infraciliature corresponds to the typical colpodid dikinetid pattern. the peculiar oral apparatus is described in detail. the feeding tube contains about 17 concentric cytostomial microtubular lamellae and postciliary microtubules. the lamellae are interconnected by dense plaques and their innermost microtubules anchor in dense material a ...201123196292
studies on the mechanism of cell elongation in blepharisma japonicum: 3. cytoplasmic calcium ions may correlate to cell elongation in calmodulin-dependent manner.among many heterotrichous ciliates, blepharisma japonicum especially demonstrates negative phototaxis in response to light stimulation, which is attributed to be caused by swimming acceleration accompanied by cell elongation and ciliary reversal. when blepharisma cells were treated with 0.1 mm egta, cell elongation gradually decreased in its degree as the adaptation time lapsed. calmodulin inhibitors such as w-7 (10(-5) m) and chlorpromazine (10(-5) m) inhibited cell elongation. these results su ...201123196293
nuclear processes in euplotes octocarinatus during conjugation.the pregamic and metagamic nuclear divisions during conjugation of euplotes octocarinatus and the development of new micro- and macronuclei were investigated with the help of phase contrast microscopy and a staining of the cells by aceto carmine. the most important stages are illustrated with microphotographs and a time table of the events based on experiments carried out at 26°c is presented. it is shown that e. octocarinatus has n = 35 chromosomes and that all of them enter the macronuclear an ...201123196294
pyrosequencing analysis of the protist communities in a high arctic meromictic lake: dna preservation and change.high arctic meromictic lakes are extreme environments characterized by cold temperatures, low nutrient inputs from their polar desert catchments and prolonged periods of low irradiance and darkness. these lakes are permanently stratified with an oxygenated freshwater layer (mixolimnion) overlying a saline, anoxic water column (monimolimnion). the physical and chemical properties of the deepest known lake of this type in the circumpolar arctic, lake a, on the far northern coast of ellesmere islan ...201223267353
emergence of the terrestrial ciliate colpoda cucullus from a resting cyst: rupture of the cyst wall by active expansion of an excystment vacuole.the first sign of excysting colpoda cucullus cells is the initiation of the pulsation of a contractile vacuole, which is then replaced by a non-pulsating vacuole (excystment vacuole) that continues to expand and finally ruptures the outermost cyst wall (ectocyst) due to inner pressure. a ciliate surrounded by flexible membranes (endocyst) thus emerges. the osmolarity of the excysting cells is estimated to be 140 mosm l(-1) from the relationship between the frequency of contractile vacuole pulsat ...201223268793
studies on trichodinid and chilodonellid ciliophorans (protozoa: ciliophora) in the indian freshwater and estuarine fishes with description of trichodinella sunderbanensis sp. nov. and trichodina nandusi sp. nov.a comprehensive icthyoparasotological survey among estuarine and freshwater fishes of west bengal, india revealed trichodinella sunderbanensis sp. nov. and trichodina acuta lom, 1961 from an estuarine fish mystus gulio (hamilton, 1822) from the vidyadhari river; trichodina nandusi sp. nov. from a freshwater fish, nandus nandus (hamilton-buchanan); chilodonella hexasticha (kiernik, 1909) kahl, 1931 from freshwater fishes labeo rohita (hamilton, 1822); and cyprinus carpio linnaeus, 1758 from the r ...201223269510
a histopathological survey of the razor clam ensis macha (pharidae) along the patagonian argentina coast.this is the first study performed to determine the health status of the razor clam, ensis macha, including six different populations along argentina patagonian coast and one of chile. the parasites and pathologies affecting e. macha were analyzed and their prevalence and mean intensity values were calculated. to establish which factors affect the presence and intensity of infection, generalized linear models (glms) were applied. basophilic inclusions, ciliates, coccidians protozoans and turbella ...201223270873
parasites of qx-resistant and wild-type sydney rock oysters (saccostrea glomerata) in moreton bay, se queensland, australia: diversity and host response.wild caught (wc) and qx resistant (qxr) sydney rock oysters were introduced at north stradbroke island and pimpama river, se queensland, australia, and sampled monthly during 1 year. three groups of parasites/diseases were identified by observation of histological sections: (1) marteilia sydneyi (queensland unknown (qx) disease) and steinhausia sp. (microsporidia) characterized by a high prevalence and deleterious impact on the host; (2) disseminated neoplasia and the trematode proctoeces sp. ch ...201223274078
morphological and molecular description of three new species of the cyrtophorid genus chlamydodon (ciliophora, cyrtophoria).this study investigates the morphology and molecular characteristics of three new cyrtophorid ciliates isolated from china seas: chlamydodon salinus n. sp., chlamydodon caudatus n. sp., and chlamydodon paramnemosyne n. sp. of these, c. salinus n. sp. differs from its congeners through a combination of body size, a cross-striated band that is not continuous, the presence of 30-34 somatic kineties, 11-15 nematodesmal rods, and 13 contractile vacuoles. chlamydodon caudatus n. sp., meanwhile, is cha ...201223289638
parasites of economically important bivalves from the southern coast of bahia state, brazil.this study investigated the parasites of three commercially important bivalve species (crassostrea rhizophorae, mytella guyanensis and lucina pectinata) from the southern coast of bahia, brazil. a total of 540 specimens were collected in august 2009 and february 2010, at three localities. the bivalve specimens were measured on their longest axis, opened, and macroscopically examined for the presence of parasites or signs of disease. they were then fixed in davidson' solution and subjected to rou ...201223295820
archaea in symbioses.during the last few years, the analysis of microbial diversity in various habitats greatly increased our knowledge on the kingdom archaea. at the same time, we became aware of the multiple ways in which archaea may interact with each other and with organisms of other kingdoms. the large group of euryarchaeal methanogens and their methane oxidizing relatives, in particular, take part in essential steps of the global methane cycle. both of these processes, which are in reverse to each other, are p ...201223326206
[about ciliates entodinium palmare and e. okoppensis (ciliophora: ophryoscolecidae) in the rumen of domestic cattles (bos taurus taurus) in the vicinity of kastamonu].the aim of this study was to determine the cytological features of the rumen ciliates entodinium palmare and e. okoppensis living in the rumen of domestic cattle in the vicinity of kastamonu, compare the morphological characters of species with their original descriptions and discuss the similarities and differences.201223339945
new stands of species of the paramecium aurelia complex (ciliophora, protozoa) in the mediterranean region (italy, greece, morocco).new stands of species of the paramecium aurelia complex are presented in the paper, p. primaurelia recorded in italy (pisa) and in morocco (marrakesh), p. biaurelia in italy (calabria), p. triaurelia in morocco (ifrane), p. pentaurelia in greece (kastorya), and p. dodecaurelia in italy (padua).201223342909
can nonloricate ciliate assemblages be a surrogate to analyze taxonomic relatedness pattern of ciliated protozoan communities for marine bioassessment? a case study in jiaozhou bay, northern china.congruency using nonloricate ciliates as a surrogate to analyze taxonomic distinctness pattern of ciliated protozoan communities for assessing water quality was studied. the results showed that (1) with significant consistency with the total ciliate communities, the spatial pattern of nonloricate ciliates was significantly correlated with the changes of environmental status; (2) four taxonomic relatedness measures were significantly correlated with those of the total ciliate communities; (3) spa ...201223356020
hydrodynamic synchronization and metachronal waves on the surface of the colonial alga volvox carteri.from unicellular ciliates to the respiratory epithelium, carpets of cilia display metachronal waves, long-wavelength phase modulations of the beating cycles, which theory suggests may arise from hydrodynamic coupling. experiments have been limited by a lack of organisms suitable for systematic study of flagella and the flows they create. using time-resolved particle image velocimetry, we report the discovery of metachronal waves on the surface of the colonial alga volvox carteri, whose large siz ...201223368623
[ciliate diversity and spatiotemporal variation in surface sediments of yangtze river estuary hypoxic zone].by using denaturing gradient gel electrophoresis (dgge) and sequencing as well as ludox-qps method, an investigation was made on the ciliate diversity and its spatiotemporal variation in the surface sediments at three sites of yangtze river estuary hypoxic zone in april and august 2011. the anosim analysis indicated that the ciliate diversity had significant difference among the sites (r = 0.896, p = 0.0001), but less difference among seasons (r = 0.043, p = 0.207). the sequencing of 18s rdna dg ...201223479889
occurrence of trichodinid ciliophorans (ciliophora: peritrichida) in the freshwater fishes of the river churni with description of trichodina glossogobae sp. nov. in west bengal, india.between january 2011 and march 2011 occurrence of trichodinid ciliophorans were studied in the freshwater fishes of the river churni. the biodiversity survey revealed presence of a new species of the genus trichodina (ehrenberg 1830) infesting glossogobius giuris (hamilton-buchanan). another freshwater fish labeo bata (hamilton 1822) were found to be infested with two species of the genus tripartiella lom 1959: tripartiella bulbosa (davis 1947) lom 1959 and t. copiosa lom 1959. adhesive disc siz ...201123542238
delimiting operational taxonomic units for assessing ciliate environmental diversity using small-subunit rrna gene sequences.delineating operational taxonomic units (otus) is a central element in any culture-independent analysis of environmental microbial eukaryotic diversity. previous studies either have not justified their choice in sequence distance used to bin small-subunit ribosomal rna (ssu rrna) gene sequences amplified from environmental samples into otus, or have used a value based on the average across a broad sampling of microbial eukaryotes. here, we analyse distances (320 922 pairwise comparisons) among s ...201123761246
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