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[specific proteins and culture media proteins found in trophozoites of e. histolytica obtained by axenic cultivation]. 19806258511
sequential histopathology of cavitary liver abscess formation induced by axenically grown entamoeba histolytica.animal models of amebic hepatic abscess should parallel the human disease. experimental amebic hepatic abscess in the hamster results in granulomatous lesions during the early period (six days) following inoculation. hamster inoculated intrahepatically with axenically grown, non-liver passaged strains of entamoeba histolytica revealed no evidence of lesions when sacrificed at later times. however, hamsters similarly inoculated with multiply liver passaged substrains were found to have large flui ...19806258512
[development of lesions produced in hamsters by inoculation of entamoeba histolytica]. 19806258513
[histochemistry of the hepatic lesion produced in hamsters by inoculation with e. histolytica from axenic cultures]. 19806258514
[participation of t lymphocytes in the anti-entamoeba histolytica immune response in hamsters]. 19806258515
[decreased activity of the mononuclear phagocytic system in hamsters infected with entamoeba histolytica]. 19806258516
loss of susceptibility to antibody and complement mediated lysis in e. histolytica. 19806258517
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. i. introduction]. 19806258518
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of e. histolytica. ii. isolation and characteristics of lysosomal antigen]. 19806258519
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. iii. counterimmunoelectrophoresis and indirect hemagglutination reactions in monkey serum]. 19806258520
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. iv. cytopathogenic effect of monkey serum on trophozoites of entamoeba histolytica]. 19806258521
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. v. intradermal reactions with histolyticin in monkeys]. 19806258522
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. vi. intrahepatic inoculation of virulent e. histolytica in monkeys]. 19806258523
[induction of immunity to amebiasis in subhuman primates by lysosomal antigen of entamoeba histolytica. vii. comments and conclusions]. 19806258524
[diagnostic and therapeutic evaluation of invasive intestinal amebiasis in children]. 19806258525
isolation of the plasma membrane of entamoeba histolytica. 19806258526
axenic cultivation of entamoeba histolytica: progress and problems.eighteen years of technological progress in the field of axenic cultivation of entamoeba histolytica is reviewed. during this period cumbersome diphasic media gave way to easily prepared clear liquid media. yields of amebae were increased a thousand fold, and doubling time reduced from 24 to less than 8 hours. attention is directed to three problems, any one of whose solution will extend the potencial of the axenic cultures. they are 1) to develop a defined medium, 2) to reduce the interval betw ...19806258527
dynamic changes on the surface of entamoeba induced by antibodies.antibody and concanavalin a bound to the cell surface of entamoeba induced segregation of these complexes towards the uroid, followed by an evagination of the membrane portion that contained the components redistributed. this vesiculated cap was released into the medium. con a polarized all con a-binding glycoproteins from the surface of the ameba while with antibodies only some of the surface antigens were movilized towards the cap area. this phenomenon was temperature dependent as shown in e. ...19806258528
role of iron-sulfur proteins in the electron transport system of entamoeba histolytica. 19806258529
purine nucleotide synthesis in entamoeba histolytica: a preliminary study.entamoeba histolytica, axenically grown, were unable to incorporate 14c-glycine into extractable purines, even after 16 hours incubation in the presence of this compound. similarly, amoebas did not incorporate 14c-sodium formate into purines. such data indicate a total absence of a de novo purine biosynthetic pathway in this amoeba. when amoebas were incubated in the presence of 3h-adenine and 3h-adenosine for one hour, considerable radioactivity was associated with amp, adp and atp; no apprecia ...19806258530
[transfer ribonucleic acids of entamoeba histolytica. i. chromatographic analysis of the trna isoacceptor species of trophozoites]. 19806258531
physico-chemical factors affecting the behaviour and activity of entamoeba histolytica.activity of individual trophozoites of several strains of axenic entamoeba histolytica under different environmental conditions was measured by means of a newly devised method. activity was found to be different between strains and corresponded to the degree of their pathogenicity. at low ph values amoebae moved further due to a straightening of their paths. a similar phenomenon was found in 1:50 cilution of pig bile. bile was also found to induce an increase in the number of monopodial, "limax- ...19806258532
[amebiasis]. 19816258893
intestinal parasites in homosexual men.a study on the prevalence of intestinal parasites in a group of homosexual men, attending a sexually-transmitted diseases clinic in glasgow, was undertaken. of 118 men examined over an eight-month period, four, one of whom had symptoms of dysentery, were found to be infected with entamoeba histolytica. cysts of iodamoeba buetschlii were also found in the stool of one of these men. a further two patients had giardiasis, and 11 men had enterobiasis. the importance of an awareness of these conditio ...19806259724
specific anti-amoebic immunoglobulins and the cellulose acetate precipitin test in entamoeba histolytica infection.using the indirect fluorescent antibody test and sera from 10 proved cases of invasive amoebiasis, the effects of absorption of igg on observed titres of amoeba-specific igm and iga were investigated. in addition, results of cellulose acetate precipitin tests were compared with anti-amoebic antibody levels. antiamoebic igm was found at titres of 1:14 to 1:112 after igg absorption in four cases. anti-amoebic iga was detected in sera from four cases, but the maximum titre was 1:28. there was no re ...19806259778
the epidemiology of entamoeba histolytica in mexico city. a pilot survey i.stocks of intestinal amoebae isolated from hospital patients in mexico city and grown in monoxenic culture were compared among themselves and with those already described (sargeaunt & williams, 1979), using the electrophoretic patterns of four enzymes: glucose phosphate isomerase (gpi), phosphoglucomutase (pgm), l-malate:nadp+ oxido-reductase (oxalacetate-decarboxylating) (me) and hexokinase (hk). new isoenzyme groups (sargeaunt & williams, 1979) of all the amoebae, including entamoeba histolyti ...19806259779
electrophoretic studies of the hexokinase of entamoeba histolytica groups i to iv. 19806259781
serologic and parasitologic studies of entamoeba histolytica in el salvador, 1974-1978.serologic and parasitologic studies were done in el salvador, c.a., from 1974-1978 to examine the reliability of the diagnosis of entamoeba histolytica infection in an endemic area and to confirm the estimates of morbidity and mortality due to amebiasis. the results suggest that infection with e. histolytica is common, but the estimated prevalence is too high. misdiagnosis occurring in hospital and public health laboratories was documented. data collected from examining family members of persons ...19816259960
study of the trophozoite surface of entamoeba histolytica with high resolution cytochemical studies and scanning electron microscopy.a study of positive and negative electrical surface charges of entamoeba histolytica trophozoites axenically cultured was carried out by means of incubation with colloidal iron solutions at ph 4.0 and 1.8, respectively. the samples were examined under the transmission electron microscope. human erythrocytes were studied using the same experimental conditions as controls. in these cells the surface negative charges were homogeneously distributed on the surface, whereas the positive charges were o ...19806260049
[an evaluation of the therapeutic action of tinidazole suppositories in intestinal infections by protozoa in children (author's transl)]. 19806260126
the laboratory diagnosis of entamoeba histolytica. 19816260273
transformation in vivo of a large race of entamoeba histolytica into a small race. 19816260274
enteric diseases.this paper focuses on the growing incidence of the "new" sexually transmitted parasitic enteric diseases: amebiasis and giardiasis. two major behavioral factors influence transmission of these diseases in the gay community: 1) oral-rectal and oral-genital sexual contact and 2) multiple sexual partners. pathogenesis, clinical signs and symptoms and complications associated with these diseases are also discussed. although many patients present with severe symptoms, approximately 50 percent of infe ...19816260431
effect of splenectomy on the size of amoebic liver abscesses and metastatic foci in hamsters.the role of the spleen in hepatic amoebiasis in hamsters was studied. in hamsters receiving an intrahepatic inoculation of 10(5) trophozoites of axenic entamoeba histolytica at 7 or 14 days postsplenectomy, the mean weight of metastatic foci increased significantly when compared with sham-splenectomized or intact controls. in contrast, when both splenectomy and intrahepatic inoculation with amoebae were carried out at the same time, there was not only a significant increase in the mean weight of ...19816260670
interaction between entamoeba histolytica and human polymorphonuclear neutrophils.the interaction of human polymorphonuclear neutrophils (pmns) with two strains of entamoeba histolytica that exhibit different degrees of "virulence" was examined in animal and tissue culture systems. pmns actively migrated toward both strains of amoebae. less virulent amoebae (strain 303) were surrounded by pmns (greater than or equal to 300/amoeba), fragmented and ingested by pmns by means that appeared to be independent of oxygen. in contrast, contact with virulent amoebae (hm1) caused loss o ...19816260869
attachment of entamoeba histolytica to glass in a defined maintenance medium: specific requirement for cysteine and ascorbic acid.cysteine and ascorbic acid were previously shown to be required by entamoeba histolytica trophozoites for attachment to glass, elongation, and ameboid movement as well as for short-term (12-24 h) survival in a balanced salt solution containing bovine serum albumin and a vitamin solution (maintenance medium 1). if the only function of cysteine and ascorbate was to decrease the redox potential, other reducing agents should be effective. however, the requirement for cysteine in the presence of asco ...19806260930
[chronic infiltration of the cecum and appendix in entamoeba histolytica infection]. 19806261462
entamoeba histolytica and giardia lamblia: growth responses to reducing agents. 19816262103
simple standardized enzyme-linked immunosorbent assay for human antibodies to entamoeba histolytica.a simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. the enzyme-linked immunosorbent assay is rapid (1 h 15 min, total incubation time), and the reported values are referenced to a positive control so that they correlate with levels of antibody s ...19816262370
interaction between entamoeba histolytica and the immune system. i. mitogenicity of entamoeba histolytica extracts for human peripheral t lymphocytes.the effect of entamoeba histolytica extracts (e.h.e.) on proliferation in vitro of human peripheral blood lymphocytes, cord blood lymphocytes, and lymphocytes enriched for t cells or non-t cells was investigated. as tested by 3h-thymidine uptake and by morphologic criteria, e.h.e. stimulate t lymphocyte proliferation. non-t lymphocytes do not respond to e.h.e. the response of lymphocytes to e.h.e. differed from their response to con a in several respects, indicating either that e.h.e. and con a ...19816262406
management of hepatic abscess. 19816263552
entamoeba histolytica and giardia lamblia: effects of cysteine and oxygen tension on trophozoite attachment to glass and survival in culture media. 19816263667
identification of igg-binding site on e.-histolytica cell membrane. 19816263722
entamoeba histolytica: a possible cause of pruritus. 19816263812
amoebic liver abscess probably acquired in birmingham, england.amoebiasis is usually acquired in countries where the disease is endemic. a case is presented of hepatic infection probably acquired indigenously with identification of the source of infection. the place of dual therapy for treatment is considered and the value of ultrasound scanning in management illustrated.19816264590
cytopathogenicity of entamoeba histolytica: trophozoite homogenates modulate dna synthesis in a mammalian cell line.we examined the effect of total trophozoite homogenates from four axenized strains of entamoeba histolytica (hk9, hm1, hm2, and hm3) on the dna synthesis of subconfluent cultures of chinese hamster ovary (cho) cells incubated at low (0.1%) serum concentration. hm1, hm2, and hm3 extracts increased [3h]thymidine incorporation to acid-insoluble material in cho cells up to a maximum of 2.5, 1.5, and 1.5 times respectively, at doses of amebal protein ranging from 16 to 125 micron/ml. hm1 and hm2 extr ...19816264701
growth inhibition of axenic entamoeba histolytica by tubercidin and ara-a.several analogs of nucleic acid components including 7-deazaadenosine (tubercidin), adenine 9-beta-d-arabinofuranoside (ara-a), 8-azaguanine, 6-azathymine, 6-mercaptopurine, 6-mercaptopurine arabinoside, 6-mercaptopurine riboside and 1,3-dideazapurine (benzimidazole) were tested for inhibition of growth of an axenic strain of entamoeba histolytica in 72 hour experiments. metronidazole and emetine were included in the experiments for comparison. tubercidin and ara-a, analogs of adenosine, were th ...19816266267
seasonal fluctuations in the occurrence of enterotoxigenic bacteria and rotavirus in paediatric diarrhoea in addis ababa.this study (march 1977-february 1978) was performed at the ethio-swedish pediatric clinic, addis ababa, to determine whether there were any seasonal fluctuations in the occurrence of diarrhoea associated with enterotoxigenic enterobacteria (eteb), rotavirus and two parasites (giardia lamblia and entamoeba histolytica).a total of 1161 children (962 patients and 199 controls) were investigated. eteb were isolated in 12.2% of the patients and 4.5% of the controls, rotavirus in 27.8% and 8%, and par ...19816266682
adhesion properties of entamoeba histolytica.trophozoites of entamoeba histolytica adhere to and phagocytize red blood cells and bacteria. furthermore, in the initial step of the amoebic infectious process the parasite attaches to intestinal epithelial cells. a lectin (carbohydrate-binding protein) which apparently has a role in the attachment of the parasite to host cells was found in trophozoites of e. histolytica. when amoeba cells were disrupted by freeze-thawing, the lectin activity, as determined by haemagglutination of human erythro ...19816266785
virulence of entamoeba histolytica in rat and its comparison with the serological responses of the amoebic patients.the virulence of 70 isolates of entamoeba histolytica was studied in the wistar strain of albino rat by intracaecal inoculation of amoebae. the results were evaluated by the neal scoring system, histopathological grading of ulcers and virulence indices. it was found that isolates from acute amoebic cases could infect and produce ulcers in significantly more animals than could the isolates from asymptomatic cyst passers. the virulence indices of isolates from acute cases were also significantly h ...19816267744
[effects of prostaglandins (pge1) on the development of amebas i experimentally infected rats]. 19806267999
[experimental amebiasis: effect of pge1 on rats infested with entamoeba histolytica].the authors have studied the effects of pge1 on rats (wistar strain) infected with entamoeba histolytica, and inoculated with pge1 either in massive dosage and killed after 3-5 and 7 days, or with daily minimum quantity of pge1 and killed on days 7 and 15 after inoculation. the results show an inhibition effect of pge1 on development and survival of amoebae in the coecum of rats inoculated with the two modalities described above. the fractionated dosage gives better results and less side effects ...19806268000
diagnosis and treatment of five parasites. enterobius vermicularis, giardia lamblia, trichuris trichiura, ascaris lumbricoides, entamoeba histolytica.general descriptions and monographs of the five parasites most likely to be seen in u. s. patients are presented. the appended monographs are designed to be a starting reference for answering drug information questions regarding these parasites. the drugs covered in the monographs are listed in the order of suggested use.19816268388
entamoeba histolytica: localization and characterization of ca2+-dependent nucleotidases. 19816268558
lectin and toxin-like activities of entamoeba histolytica: comparison of properties.a comparison was made between properties of a recently discovered entamoeba histolytica lectin which has a carbohydrate specificity for n-acetylglucosamine oligosaccharides and the previously found toxin-like principle of the ameba. a separation between these two activities was achieved upon subcellular fractionation by high speed centrifugation of freeze-thawed disrupted e. histolytica trophozoites (strain hm-1). practically all of this lectin activity, as determined by hemagglutination of glut ...19816269446
[focus of amebiasis in a psychiatric hospital in the east of france].at the occasion of the discovery of an amoebiasis focus in a psychiatric hospital (36,2 % of the patients were entamoeba histolytica carriers), the authors insist on the importance of systematical and continued coprological controls. they observe that the two means of fight used together, the environment and the mass treatment, have strongly limited the propagation of parasitosis. by this way they note efficiency of the association of the medicinal polyvalent and contact amoebicides.19816269506
glucose dissimilation in entamoeba histolytica.the endproducts of glucose dissimilation in entamoeba histolytica were found to be carbon dioxide, acetate and ethanol. this organism possesses the same embden-meyerhof intermediates as found in most investigated organisms. in its glucose-to-pyruvate pathway several unusual features were demonstrated. the pyruvate-to-acetate and pyruvate-to-ethanol pathways are also unique as compared with that of other organisms. the distinctive properties of the metabolic enzymes provide the basis for the deve ...19816269810
[cervico-uterine amebiasis. characteristics of 15 cases and review of the literature]. 19806270758
inhibition of clonal growth of giardia lamblia and entamoeba histolytica by metronidazole, quinacrine, and other antimicrobial agents. 19816271724
role of adherence in cytopathogenic mechanisms of entamoeba histolytica. study with mammalian tissue culture cells and human erythrocytes.the enteric pathogen, entamoeba histolytica, appears to cause disease by adhering to and then destroying mucosal barriers. using an in vitro method of studying the interaction of e. histolytica with target cells (chinese hamster ovary [cho] and human erythrocytes [rbc]), we examined the mechanism of amebic adherence and its role in lysis of target cells. killing and phagocytosis of target cells by amebas ceases at 4 degrees c, allowing observation of adherence. amebas adhere to cho cells at 4 de ...19816271810
studies on amoebiasis in symptomless carriers. 19816271876
a calcium regulated adenosine triphosphatase in entamoeba histolytica.axenically grown trophozoites of entamoeba histolytica (nih-200 strain) contain an active atpase (170 nmol po4/min per mg protein) with maximal activity at ph 8.8, a high affinity for atp (km approximately 40 micro m) and an absolute and specific requirement for ca2+. the activation by ca2+ shows positive cooperativity (nh = 2.48) at calcium concentrations below 8 micro m and no cooperativity between 8 and 25 micro m. the latter concentration fully saturates the enzyme. the observed activity is ...19816272107
susceptibility of entamoeba histolytica to oxygen intermediates.to explore the susceptibility of the extracellular protozoan, entamoeba histolytica, to toxic oxygen intermediates, trophozoites were exposed to fluxes of o2, h2o2, and oh. generated enzymatically by the glucose oxidase and xanthine oxidase reactions. hm-1 trophozoites were resistant to o2, but were readily killed by h2o2 alone. oh. and 1o2 were not required for effective amebicidal activity. the addition of peroxidase and halide enhanced trophozoite killing by h2o2. sonicates of amebae containe ...19816272108
left-side pleuropulmonary amoebiasis: a case report from chad. 19816272459
metronidazole metabolism in cultures of entamoeba histolytica and trichomonas vaginalis.acetamide forms from metronidazole in cultures of either entamoeba histolytica or trichomonas vaginalis as it does in cultures of bacteria which are susceptible to this drug. under aerobic conditions, the formation of acetamide is more rapid in a strain of t. vaginalis which is more susceptible to metronidazole. thus, it appears that the formation of acetamide may be associated with the microbiocidal action of metronidazole.19816272633
effect of a low protein diet on rats experimentally infected with entamoeba histolytica. 19816272659
indirect fluorescent antibody test in the differential diagnosis of hepatic amoebiasis from non-specific hepatomegaly. 19816273311
use of glutaraldehyde-treated sheep erythrocytes in indirect haemagglutination test for amoebic coproantibody. 19816273312
search for new antiamebic agents. i. synthesis and bioassay of new schiff bases of 1-phenyl-2-morpholinoethylidene-1-urea.as potential agents against e. histolytica 19 new schiff bases of 1-phenyl-2-morpholinoethylidene-1-urea were synthesized by the condensation of the parent compounds with various carbonyl compounds. thirteen of them were tested against the axenic culture of e. histolytica at concentration 125 microgram/cm3 but no significant amoebicidal activity was observed.19816273830
dynamics of the interaction between entamoeba histolytica and components of the immune response. iii. fate of antibodies after binding to the cell surface.binding to the surface of living e. histolytica trophozoites of 125i-labelled anti-amoeba antibodies or of such antibodies and antiglobulin resulted in a rapid disappearance of the antibody into the medium. this disappearance consisted of a rapid temperature-independent and a subsequent slow temperature-dependent phase. the disappearance of antibody from fixed trophozoites merely consisted of a temperature-independent rapid phase whereas no slow phase was observed. the magnitude of the rapid pha ...19816273999
amoebiasis as a cause of death in a scottish resident.an elderly woman, resident throughout her life in scotland presented with abdominal pain and diarrhoea. a provisional diagnosis of ulcerative colitis was made on sigmoidoscopy. at post mortem entamoeba histolytica was demonstrated in considerable numbers in the mucosal ulcers. it would appear though extremely rare that amoebiasis should be considered in the differential diagnosis of colonic ulceration.19816274015
the collagenase of entamoeba histolytica.the present work was designed to investigate the capacity of trophozoites of entamoeba histolytica to adhere to and digest human collagen types i and iii in vitro. the time-course of binding of ameba to both human collagen types i and iii was similar. however, the kinetics of detachment were different for each collagen type. trophozoites of e. histolytica cultured on heat-reconstituted type i collagen gels produced a well-defined area of lysis. quantitative studies using 14c-labeled collagen rev ...19826274995
dysentery form gastroenteritis in infancy. 19816275330
use of enzyme linked immunosorbent assay in intestinal and extra-intestinal amoebiasis (amoebic liver abscess).the sera of patients with amoebic liver abscess, intestinal amoebiasis and non-specific hepatomegaly were examined for the presence of antibodies to entamoeba histolytica antigen by enzyme linked immunosorbent assay (elisa). all the amoebic liver abscess sera were strongly positive. the test was also positive in all the proved cases of intestinal amoebiasis but the elisa values were relatively slightly lower. nine of 10 cases of non-specific hepatomegaly with stools negative for e. histolytica g ...19816275581
[epidemiological role of blattella germanica (l.) in amebiasis]. 19816275613
a study of intestinal protozoa including non-pathogenic entamoeba histolytica from patients in a group of mental hospitals.twenty-five per cent of 174 patients residing in three mental hospitals were found to be infected with protozoa. seven per cent of the patients were infected with entameba histolytica. isoenzyme characterization of the e. histolytica grown in culture showed that there were no pathogenic zymodemes of this organism in any of the specimens tested. (am j public health 1982;72:178-180.)19826275721
a seroepidemiological study of amebiasis in adults in maracaibo, venezuela.eight hundred and forty-eight sera collected from adult patients attending the central and university hospitals and from blood donors at the blood bank in maracaibo, venezuela, were screened for serologic evidence of entamoeba histolytica infection, employing the indirect hemagglutination test. the seropositivity rates obtained ranged from 4.4-6.5% and most of the positive sera showed low titers of 1:128-1:256. a predominance of positive reactors was observed in the first decades of life with a ...19816275725
adhesion of entamoeba histolytica trophozoites to monolayers of human cells.the adhesion of radiolabeled trophozoites of entamoeba histolytica to monolayers of a human intestinal epithelial cell line was found to be dependent on time, temperature, and concentration. adherence seems to be mediated by a carbohydrate binding protein (lectin), which was previously found in trophozoites of e. histolytica. the adherence of the trophozoites was found to be ph-dependent and was inhibited by several n-acetyl-glucosamine-containing glycoconjugates, such as bacterial peptidoglycan ...19816276476
[clinical and therapeutic study of a group of 217 patients with intestinal giardiasis and amebiasis].in 15 months of the years 1979-1980, 158 patients with giardiasis and 69 patients with intestinal amebiasis were diagnosed and treated in the outpatient department of the swiss tropical institute. repeated examination of merthiolate-iodine-formol-fixed stools was the method of choice for detecting intestinal protozoa. parasitological investigation of duodenal fluid (enterotest) was less effective. fewer than 30% of the cases were symptomatic, usually mentioning diarrhea (73-85%) or abdominal dis ...19816276972
quantification of amebae specific antibodies as "multiple of normal activity (mona)" with a standardized enzyme immunoassay (eia).sera of 853 returnees from tropical countries, of 24 cases with amebic liver abscess and of 172 nonexposed german individuals were tested for antibodies to e. histolytica in the enzyme immunoassay (eia). antibody results were expressed as "multiple of normal activity (mona)". the qualification of the eia as a routine screening procedure for amebae specific antibodies was investigated and compared to that of the complement fixation test. based on the symmetric frequency distribution of results fr ...19816277100
rapid diagnosis of amoebic liver abscess using entamoeba histolytica antigen. 19816277264
importation of pathogenic entamoeba histolytica. 19826277421
cholesterol induced changes in concanavalin a agglutinability of entamoeba histolytica. 19816277782
new quinoline derivatives as amoebicidal and cysticidal agents. 19816278460
comparison of indirect fluorescent-antibody amoebic serology with counterimmunoelectrophoresis and indirect hemagglutination amoebic serologies.patients ranged from those with no prior diagnosis of or suspected exposure to entamoeba histolytica to those with proven amoebic liver abscesses (extraintestinal disease). a comparison of serologies from patients with proven and suspected amoebiasis or possible past exposure revealed good correlation between the indirect fluorescent antibody (ifa) procedure and the other methods used, counterimmunoelectrophoresis and indirect hemagglutination. titers from patients with proven extraintestinal am ...19826279694
amoebiasis: a review. 19826279839
isolation and diagnostic cultivation of entamoeba histolytica: a comparative study of two methods. 19816280013
parasitic bowel disease: three pathogens important in primary care.a high index of suspicion and careful application of diagnostic methods are essential for accurate diagnosis of parasitic bowel diseases. the varied clinical spectrum of giardiasis, amebiasis, and strongyloidiasis emphasizes the need to consider these pathogens when patients present with gastrointestinal complaints. giardiasis should be suspected in patients, especially returned travelers, with unexplained increase in stool frequency, particularly with bloating, flatulence, or vague systemic sym ...19826280160
comparative electron microscopic study of an imported pathogenic strain and an autochthonous nonpathogenic strain of e. histolytica with particular reference of nuclear inclusions. 19816280639
isolated entamoeba histolytica actin does not inhibit dnase-i activity. 19826280688
in vitro amoebicidal activity of immune cells.the amoebicidal activity of peripheral blood lymphocytes and spleen and peritoneal cells from hamsters vaccinated against or protected from hepatic amoebiasis and from those with hepatic amoebiasis was investigated. peripheral blood lymphocytes and peritoneal and spleen cells from vaccinated or protected animals can kill trophozoites of entamoeba histolytica in vitro. in contrast, spleen cells from infected hamsters showed no significant cytotoxic effect on the parasite. these data suggest that ...19826281189
attachment and ingestion of bacteria by trophozoites of entamoeba histolytica.entamoeba histolytica trophozoites were found to be very selective in their interactions with bacteria. two principal mechanisms were shown to be responsible for these interactions. certain bacteria, such as a number of escherichia coli and serratia marcescens strains which are known to contain mannose-binding components on their cell surface, bound to mannose receptors on the amoeba membrane. this attachment was markedly inhibited by alpha-methylmannoside (0.5%), especially when the incubations ...19826281192
the presentation of amoebiasis. 19826281593
cellulose acetate membrane precipitin (cap)test in amoebiasis using antigens from four different strains of entamoeba histolytica.a simple cellulose acetate membrane precipitin (cap) test was evaluated against immunoelectrophoresis (iep) test using saline extract from 4 different strains (hk-9, ht-10, ht-12 and ht-31) of axenically grown entamoeba histolytica as the antigens. all 81 sera from patients with amoebic liver abscess were positive in the iep against the antigens from all 4 strains. with the cap test the number positive against antigens from hk-9, ht-10, ht-12 and ht-31 were 79,77,79 and 71 respectively sera from ...19816281923
the diagnostic significance of the indirect haemagglutination test in amaebiasis in children. 19826282994
electrophoretic isoenzyme patterns of entamoeba histolytica and entamoeba chattoni in a primate survey.stocks of entamoeba histolytica grown in a monoxenic culture system from the feces of nonhuman primates are compared with the eleven zymodemes of e. histolytica so far demonstrated from man. in a similar fashion, entamoeba chattoni has also been grown and identified. both e. histolytica and e. chattoni have been demonstrated in keepers of the primate collections. comparisons have been made using the electrophoretic patterns of three enzymes: glucosephosphate isomerase [(gpi) e.c.5.3.1.9], phosph ...19826283074
homosexual transmission of amoebiasis. 19826283081
an outbreak of amebiasis spread by colonic irrigation at a chiropractic clinic.from june 1978 through december 1980, at least 36 cases of amebiasis occurred in persons who had had colonic-irrigation therapy at a chiropractic clinic in western colorado. of 10 persons who required colectomy, six did. of 176 persons who had been to the clinic in the last four months of 1980, 80 had received other forms of treatment. twenty-one per cent of the colonic-irrigation group had bloody diarrhea, as compared with 1 per cent of the non-irrigation group (p = 0.00013). thirty-seven per c ...19826283354
sero-epidemiology of amoebiasis in peninsular malaysia. 19826284074
seroconversion to entamoeba histolytica among short-term travelers to mexico.paired serum samples were evaluated for the presence of antibodies to entamoeba histolytica in 326 healthy college students from the united states who lived in guadalajara, mexico, for an average of four weeks. one hundred eighty of these students had an enteric illness develop, but no stool test results were positive for ameba. an indirect hemagglutination assay was the serologic method used, and no seroconversion was demonstrates. this finding confirms the belief that amebiasis is a rare cause ...19826284082
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