Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| effect of co2+-substitution on the substrate specificity of phospholipase c from bacillus cereus during attack on two membrane systems. | phospholipid degradation by native phospholipase c from bacillus cereus and enzyme forms where one or both of the zn2+ prosthetic groups had been replaced with co2+ was studied in human erythrocyte membranes (ghosts) and resuspended freeze-dried bovine brain myelin. the rate of total phospholipid degradation was 2-9-fold more rapid with erythrocytes than with myelin. with both membrane systems the activity decreased in the order znzn-enzyme greater than znco-enzyme greater than coco-enzyme. for ... | 1982 | 6817748 |
| cardiolipin-protein interactions in the phosphate binding activity of a proteolipid from yeast mitochondria. action of phospholipases a2 and c and of cations. | a proteolipid able to bind phosphate has been isolated from yeast mitochondria. during the purification the active protein was always associated with cardiolipin. the cardiolipin requirement for the phosphate binding activity of this proteolipid has been studied using controlled lipid depletion with two phospholipases a2 and with phospholipase c. only phospholipase a2 from pig pancreas, that deacylated cardiolipin, promoted inhibition of the proteolipid activity (but never more than 70 per cent) ... | 1982 | 6817819 |
| substrate specificities of adenosine deaminase and adenosine phosphorylase from bacillus cereus. | the substrate specificity of two adenosine metabolizing enzymes from bacillus cereus has been investigated, using partially purified preparations. adenosine deaminase is shown to be highly specific for adenosine (km = 5.6 x 10(-5) m at ph 8.1); 2'-deoxyadenosine, formycin a and 2-amino-adenosine are deaminated by the enzyme preparation, but reaction rates are at least 20 times lower than that for adenosine at concentrations up to 0.1 mm, due to higher km and/or lower vmax values. 3'-deoxyadenosi ... | 1982 | 6818177 |
| in vitro effect of phospholipase c from bacillus cereus on tissue thromboplastin from different species. | purified phospholipase c from bacillus cereus caused a significant loss in the procoagulant activity of thromboplastin preparations from man, rabbit, sheep, cow, rat and mouse. however, marked differences were observed with respect to the degree of inactivation. rat, mouse, bovine and one type of rabbit preparations (prepared from acetone powdered brain) were markedly more sensitive to attack by phospholipase c than were preparations of human, sheep and standard rabbit preparations. the relative ... | 1982 | 6818711 |
| [organic pathomorphological changes in animals with dietary infection by food products contaminated by bacillus cereus]. | the authors noticed marked vascular disorders in the capillary system, focal hemorrhages, and inflammatory, largely proliferative reactions in the stroma coupled with dystrophic and necrotic changes in parenchymal elements. the changes were found to be the most pronounced in the intestine, liver, myocardium, and in brain tissue. the degree of the lesions depended on the microorganism dosage. provided the food contained 10(9)/g bac. cereus, the injuring action was recorded to be more powerful, wh ... | 1982 | 6818766 |
| inactivation of beta-lactamase i from b. cereus 569/h with phenylglyoxal, an arginine-selective reagent. | 1982 | 6818958 | |
| assessment of immunofluorescence measurements of individual bacteria in direct and indirect assays for bacillus anthracis and bacillus cereus spores. | 1982 | 6819288 | |
| [survey of various groups of microorganisms in salads with mayonnaise obtained in restaurants, snack bars and rotisseries]. | 1982 | 6820571 | |
| [microbiological contamination of desserts prepared by the gastronomic establishments]. | 1982 | 6820847 | |
| the 1h nuclear-magnetic-resonance spectroscopy of cobalt(ii)-beta-lactamase ii. | the 1h n.m.r. spectra of beta-lactamase ii in the presence of co(ii) were studied. analysis of the spectra suggests that co(ii) binds at the same two metal-binding sites as does zn(ii). the binding of co(ii) at the first site is much weaker than the binding of zn(ii) at this site, whereas the binding of co(ii) at the second site is tighter than the binding of zn(ii). the binding of co(ii) to the mono-zinc(ii)-enzyme caused only one marked change in the spectrum, namely a decrease in the intensit ... | 1980 | 6821370 |
| characterization of intracellular inclusions formed by pseudomonas oleovorans during growth on octane. | the growth of pseudomonas oleovorans on n-octane was characterized by the formation of intracellular structures. these inclusions were isolated and characterized. morphologically, they resembled the poly-beta-hydroxybutyrate granules found in bacillus cereus, as shown by freeze-fracture electron microscopy. the elemental analysis of isolated granules showed, however, that they do not contain poly-beta-hydroxybutyric acid. instead, the analysis was consistent with a c8 polyester, which interpreta ... | 1983 | 6841319 |
| effects of phospholipase c on the na+-ca2+ exchange and ca2+ permeability of cardiac sarcolemmal vesicles. | we have examined the effects of phospholipase c pretreatment on ca2+ transport in highly purified canine cardiac sarcolemmal vesicles. na+-ca2+ exchange, measured as nai+-dependent ca2+ uptake, is stimulated when 10-70% of the membrane phospholipid has been hydrolyzed. although the phospholipase c treatment also increases sarcolemmal passive ca2+ flux, the membrane maintains a sufficient permeability barrier for enhanced transport (via na+-ca2+ exchange) to be observed. these effects can be obta ... | 1983 | 6853558 |
| asymmetry of lipid organization in cholinergic synaptic vesicle membranes. | the lipid composition of purified torpedo cholinergic synaptic vesicles was determined and their distribution between the inner and outer leaflets of the vesicular membrane was investigated. the vesicles contain cholesterol and phospholipids at a molar ratio of 0.63. the vesicular phospholipids are (mol% of total phospholipids): phosphatidylcholine (40.9); phosphatidylethanolamine (24.6); plasmenylethanolamine (11.5); sphingomyelin (12); phosphatidylserine (7.3); phosphatidylinositol (3.7). the ... | 1983 | 6870819 |
| aerobic mesophilic and psychrotrophic sporeforming bacteria in buffalo milk. | seasonal variation of the population of aerobic sporeformers in raw milk was higher in summer than in other seasons. least variation was in fall, but variation in winter and spring was similar. aerobic mesophilic sporeformers in raw milk consisted mainly of bacillus subtilis (42.5%) and bacillus megaterium (34.8%), followed by bacillus circulans (4.9%), bacillus cereus (4.6%), bacillus pumilus (2.9%), bacillus polymyxa (2.8%), bacillus licheniformis (1.9%), bacillus badius (1.5%), bacillus brevi ... | 1983 | 6886164 |
| properties of an acid phosphatase in pulmonary surfactant. | lung surfactant, a lipid-protein complex purified from dog lungs, contains a highly active phosphomonoesterase associated with it. this phosphatase is quite specific for the hydrolysis of phosphatidic acid and 1-acyl-2-lysophosphatidic acid. the enzyme possesses many of the characteristics of the microsomal enzyme, phosphatidate phosphohydrolase (ec 3.1.3.4). in addition, we have shown that this enzyme will also convert phosphatidylglycerol phosphate [1-(3-sn-phosphatidyl)-sn-glycerol-1-p] to ph ... | 1980 | 6928679 |
| cholesterol solubilization by short-chain lecithins: characterization of mixed micelles and cholesterol oxidase activity. | the synthetic short-chain lecithins diheptanoylphosphatidylcholine and dioctanoylphosphatidylcholine solubilize cholesterol up to 10 and 18 mol %, respectively. the half-time for diheptanoylphosphatidylcholine solubilization of solid cholesterol is 80 (+/- 30) min. this is much faster than triton x-100 micelle or egg lecithin vesicle solubilization of solid cholesterol. both the broadening of lecithin and [4-13c]cholesterol carbon resonances by mn2+ and the observation of surface dilution kineti ... | 1981 | 6947824 |
| properties of ro 13-99041 as a substrate and inhibitor of beta-lactamases. | ro 13-9904 is a novel semisynthetic and highly active parenteral cephalosporin. its stability against hydrolysis by several beta-lactamases was studied. the enzymes were isolated from various enterobacteriaceae or staphylococcus aureus and several commercially available enzyme preparations were also included. most of the penicillinases, cephalosporinases or the tem-type beta-lactamase studied were unable to hydrolyze this novel cephalosporin. the cephalosporinases from bacillus cereus 569/h9 and ... | 1981 | 6972858 |
| interaction of beta-lactamase of streptomyces cacaoi. i. clavulanic acid and ps-5. | inactivation of a beta-lactamase of streptomyces cacaoi by clavulanic acid and ps-5 was investigated and compared with that of a beta-lactamase of bacillus cereus. inhibition of the enzymes induced by clavulanic acid and the beta-lactam antibiotic ps-5 was found to be progressive with time. however, the degree of inhibition of the beta-lactamase from s. cacaoi increased more progressively with time than that of the enzyme from b. cereus. conformative response constants were determined. as compar ... | 1981 | 6975771 |
| in vitro evaluation of pyridine-2-azo-p-dimethylaniline cephalosporin, a new diagnostic chromogenic reagent, and comparison with nitrocefin, cephacetrile, and other beta-lactam compounds. | pyridine-2-azo-p-dimethylanaline cephalosporin (padac), a chromogenic reagent which is purple and changes to yellow upon cleavage of its beta-lactam ring, was evaluated in comparison with other chromogenic cephalosporins. padac exhibited little antimicrobial activity against gram-negative bacteria, but did have good activity (minimum inhibitory concentration, 0.12 to 0.5 microgram/ml) against staphylococcus aureus, a quality comparable to nitrocefin. nitrocefin, however, demonstrated an unexpect ... | 1982 | 6978350 |
| conjugation of enzymes to immunoglobulins using dimaleimides. | a method is described for coupling enzymes to immunoglobulins using sulphydryl derivatives of the proteins and a dimaleimide which is relatively water-soluble. parameters affecting the performance of the conjugates have been examined including level of sulphydryl incorporation, ratio of enzyme/immunoglobulin and nature of dimaleimide used. peroxidase-immunoglobulin conjugates made by the dimaleimide method have been compared with those made by the periodate oxidation method and found to have a s ... | 1980 | 6988011 |
| [investigations on the sensitivity of the detection of chlortetracycline (ctc) in blood and medicated feed within the official prophylaxis and therapy of psittacosis, using the bacillus cereus-inhibition-test in agar-plates (author's transl)]. | 1981 | 7016496 | |
| penem derivatives: beta-lactamase stability and affinity for penicillin-binding proteins in escherichia coli. | penem derivatives, a new group of beta-lactam antibiotics with potent activities against a wide range of bacteria, including pseudomonas aeruginosa, were tested for their stability against hydrolysis by beta-lactamases purified from clinical isolates of morganella morganii. proteus vulgaris, and escherichia coli and by a penicillinase from bacillus cereus. penems having 6 alpha substituents, such as hydroxyethyl, hydroxymethyl, and ethyl groups, were very stable against hydrolysis by each of the ... | 1982 | 7049076 |
| relationship of the syntheses of spore coat protein and parasporal crystal protein in bacillus thuringiensis. | two major classes of polypeptides were extracted from the spore surface of bacillus thuringiensis subsp. kurstaki: the 134,000-dalton protoxin that is the major component of the crystalline inclusion and spore coat polypeptides very similar to those found on bacillus cereus spores. the quantity of spore coat polypeptides produced was reduced when compared with that produced by certain acrystalliferous mutants or by b. thuringiensis subsp. israelensis. the latter organism produced an inclusion to ... | 1982 | 7085565 |
| antibacterial substance from carica papaya fruit extract. | ripe and unripe carica papaya fruits (epicarp, endocarp, seeds and leaves) were extracted separately and purified. all the extracts except that of leaves produced very significant antibacterial activity on staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa and shigella flexneri. the mic of the substance was small (0.2-0.3 mg/ml) for gram-positive bacteria and large (1.5-4 mg/ml) for gram-negative bacteria. the substance was bactericidal and showed properties of a pr ... | 1982 | 7097295 |
| topological location and biological significance of phospholipids in the membrane of newcastle disease virus. hydrolysis of phospholipids in intact virion with pure phospholipases a2, c, and d. | the composition, topological distribution and biological significance of phospholipids in the membrane of newcastle disease virus (ndv) grown in embryonated chicken eggs were investigated. phosphatidylethanolamine and sphingomyelin were the predominant phospholipids in ndv membrane. the location of phospholipids in the lipid bilayer of the membrane was studied by assessing their reactivities with highly purified phospholipase a2 (agkistrodon halys blomhoffi) and phospholipase d (streptomyces chr ... | 1982 | 7130158 |
| toxicity of phospholipase c in rabbits. | tissue thromboplastin is the most potent physiological trigger of blood coagulation and is probably involved in the pathogenesis of several forms of intravascular coagulation. phospholipase c from bacillus cereus is an effective inhibitor of thromboplastin. as part of an investigation into the possible use of phospholipase c as a therapeutic or prophylactic agent in thrombosis and other forms of intravascular coagulation, we have previously studied its effect in rats. we now report on the toxici ... | 1982 | 7134808 |
| [lipase and phospholipase from staphylococcus aureus of different origin. ii. purification and characterization (author's transl)]. | lipase and phospholipase c from staphylococcus aureus could be isolated by gel filtration on sephacryl s 200 (fig. 1a, b) and completely separated by refiltration under the same conditions. isoelectric focusing gave maximal enzyme-activities for lipase at ph 8.6 and 9.5 and for phospholipase c at ph 7.4 (fig. 2). thin-layer chromatography revealed that the reaction products in lecithin agar of the phospholipase c-preparations from s. aureus and bacillus cereus were identical (table 1). | 1980 | 7223122 |
| generalized transduction in bacillus thuringiensis var. berliner 1715 using bacteriophage cp-54ber. | a phage isolated from lysates of phage cp-54 grown on bacillus cereus 569 and selected on the basis of its ability to infect bacillus thuringiensis var. berliner 1715 (serotype i) was designated cp-54ber. phages cp-54ber and cp-54 were similar in size, morphology, cryosensitivity and stabilization by dimethyl sulphoxide. they showed significant differences with regard to inactivation by specific antiserum, adsorption to the berliner strains and host range. phage cp-54ber was able to mediate gene ... | 1980 | 7252480 |
| glysperin, a new antibiotic complex of bacterial origin. i. production, isolation and properties. | strains of bacillus cereus produced a complex of new antibiotics, glysperins a, b and c. they are basic, water-soluble antibiotics and active against gram-positive and gram-negative bacteria including aminoglycoside-resistant organisms. glysperin a is a major component of the antibiotic complex and approximately two to four times more active than components b and c. | 1981 | 7275817 |
| the specificity requirements of bacteriophage t4 lysozyme. involvement of n-acetamido groups. | bacillus cereus peptidoglycan with n-unsubstituted glucosamine residues was insensitive to treatment with bacteriophage t4 lysozyme. after n-acetylation with acetic anhydride, t4 lysozyme cleared solutions of the peptidoglycan and reducing sugars were liberated. the digestion products were mainly of high molecular weight, since the peptidoglycan is peptide cross-linked to a great extent. n-propylation did not convert the partially n-unsubstituted peptidoglycan to a sensitive form. it is conclude ... | 1981 | 7308203 |
| a novel n-acetylglucosaminyl polyprene in hen oviduct. | incubation of hen oviduct membranes with udp-[14c]n-acetylglucosamine (glcnac) leads to the formation of four endogenously labeled lipids. one of these lipids has not previously been characterized. based on its chromatographic behavior on sg-81 paper and deae-cellulose, it is a monophosphate diester. the oviduct lipid is extremely sensitive to mild acid hydrolysis and, based on a comparison with alpha-glcnac-phosphoryl-undecaprenol from bacillus cereus, the oviduct lipid contains glcnac linked t ... | 1980 | 7400130 |
| composition and properties of the cell wall of methanospirillum hungatii. | dithiothreitol reacted, at ph 9.0, with the isolated cell walls of methanospirillum hungatii, to release about 23% of the cell wall dry weight as a high molecular weight fraction (> 0.5 million daltons). untreated walls consisted of 70% amino acids, 11% lipid, and 6.6% carbohydrate. sugars were identified as rhamnose, ribose, glucose, galactose, and mannose. the wall material that was released contained only 47% amino acids and was enriched in lipid, glucose, and phosphate. these results support ... | 1980 | 7407700 |
| ageing of blood in hereditary spherocytosis. | blood from 5 patients suffering from hereditary spherocytosis (hs) was stored in acid-citrate-dextrose anticoagulant at 4 degrees c for up to 6 weeks. the erythrocyte morphology and susceptibility to lysis by phospholipase c (bacillus cereus) were examined at 2-weekly intervals and compared with erythrocytes from 6 different donations of stored normal blood. the hereditary spherocytes went through essentially the same series of morphological changes as did normal erythrocytes. fresh hereditary s ... | 1980 | 7466302 |
| chromogenic assay for phospholipase c from bacillus cereus. | a standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (km and vmax) for phosphatidylcholine-preferring phospholipase c from bacillus cereus (plcbc). the assay is applied to the determination of the ki for a known inhibitor of plcbc. | 1995 | 7485988 |
| cloning and primary structure of a new hemolysin gene from bacillus cereus. | a new hemolysin gene from bacillus cereus vkm-b164 was cloned in escherichia coli and sequenced. deduced protein consists of 219 amino acids and has a molecular mass of 24.4 kda. it has been concluded that the hemolysin, named 'hemolysin iii', is distinct from the b. cereus hemolysins reported previously: cereolysin, sphingomyelinase, cereolysin ab, hemolysin ii, and 'cereolysin-like' hemolysin (honda, t., shiba, a., seo, s., yamamoto, j., matsuyama, j. and miwatani, t. (1991) fems microbiol. le ... | 1995 | 7495855 |
| antibacterial activity of helichrysum aureonitens (asteraceae). | the antibacterial activity of extracts from helichrysum aureonitens was investigated. the dichloromethane extract was active against all five gram positive bacteria tested and the methanol extract was active only against bacillus cereus, b. pumilus and micrococcus kristinae, while the water extract had no activity against any of the organisms. none of the extracts inhibited the growth of the five gram negative bacteria tested. | 1995 | 7500636 |
| [intracerebral hemorrhage and multiple brain abscesses caused by bacillus cereus within the scope of acute lymphatic leukemia]. | multiple hematogenic brain abscesses in immunosuppression are occasionally caused by rare and primary apathogenic causative agents. we report a first case of an isolated cns infection by bacillus cereus, which led to death from multiple brain abscesses and an intracerebral hemorrhage, probably caused by the infection, within 4 days. the underlying disease leading to immunosuppression was acute lymphatic leukemia in complete remission. in spite of antibiotic therapy the chemotherapy-induced neutr ... | 1995 | 7501096 |
| a rapid method to quantify bacterial contamination on hatching eggs. 1. correlation of optical density with initial bacterial count. | the use of optical-density (od) readings after a 6-h incubation period, as a suitable method to quantify the bacterial contamination on hatching eggs, was established by the use of pure cultures of five bacterial isolates found to be the most prevalent on the hatching eggs examined. these isolates were identified as micrococcus luteus, staphylococcus gallinarum, streptococcus epidermidis, pseudomonas cepacei and bacillus cereus. it was established that the od reading was repeatable when the same ... | 1994 | 7501365 |
| [tyrosol--the autoregulatory d1 factor of the yeast saccharomyces cerevisiae]. | the autoregulatory d1 factor of the yeast, saccharomyces cerevisiae, that induces the transition of vegetative cells into refractory resting forms, has been isolated from the cell-free culture medium as an individual crystalline compound. it has been shown to be 2-(4-hydroxyphenyl)ethane-1-ol which is also known as tyrosol. when added to the producer culture at 5-15 microm concentration, tyrosol stimulated the endogenous respiration of cells, but inhibited at 20-80 microm concentration. at 200-8 ... | 1993 | 7506347 |
| a study of purified pyocyanine produced by pseudomonas aeruginosa: properties and grouping. | a study of more than one thousand strains of pseudomonas aeruginosa was performed. these were collected from different sources, i.e. humans, animals, environment and food. cumulative results revealed the existence of three different groups of pyocyanine. the biological importance of grouping this pigment is due to its activity against other bacteria in comparison with other antibiotics such as cyanomycin produced by streptomyces cyanoflavus. no such grouping or any other classification was found ... | 1993 | 7516618 |
| platelet bacterial contamination and the use of a chemiluminescence-linked universal bacterial ribosomal rna gene probe. | currently, the maximum outdate for platelets is 5 days, because of the increasing chance of bacterial growth over time. various methods for rapid detection of bacterial contamination of blood components have been described, with mixed results and no general acceptance. a recently described, molecular biologic approach for the detection of bacterial contamination involves a chemiluminescence-linked universal dna bacterial probe to a highly conserved bacterial region of ribosomal rna (rrna). | 1994 | 7522362 |
| specific antibody-mediated detection of brochothrix thermosphacta in situ in british fresh sausage. | a rabbit polyclonal antibody-linked probe was developed which detected 76% of 800 food isolates of the spoilage bacterium brochothrix thermosphacta when cells were bound to nitrocellulose. in slide cross-reaction tests all six environmental isolates tested were stained but the type strain was not. the antibody did not cross-react with listeria grayi, l. monocytogenes, lactobacillus plantarum, lactococcus lactis, streptococcus mutans, bacillus cereus or b. subtilis. the antibody-linked probe dete ... | 1995 | 7538105 |
| comparative analysis of the 16s to 23s ribosomal intergenic spacer sequences of bacillus thuringiensis strains and subspecies and of closely related species. | bacillus thuringiensis spacer regions between the 16s and 23s rrnas were amplified with conserved primers, designated 19-mer and 23-mer primers. a spacer region of 144 bp was determined for all of 6 b. thuringiensis strains, 7 b. thuringiensis subspecies, and 11 b. thuringiensis field isolates, as well as for the closely related species bacillus cereus and bacillus anthracis. computer analysis and alignment of nucleotide sequences identified three mutations and one deletion in the intergenic spa ... | 1995 | 7538281 |
| activity and stability of bacillus cereus penicillinase entrapped in aerosol ot reverse micelles. | the kinetics of enzyme catalyzed hydrolysis of penicillin g and stability of the enzyme alpha-penicillinase, entrapped in aerosol ot reverse micellar droplets have been investigated spectrophotometrically. various physical parameters, such as, water pool size (related to wo), ph and temperature, were optimized for maximum activity of penicillinase in water/aerosol ot/isooctane reverse micelles. the enzyme showed maximum activity of wo - 14 and ph, 7.0. at any temperature the enzyme was to be mor ... | 1995 | 7543871 |
| culture conditions that influence accumulation of zwittermicin a by bacillus cereus uw85. | bacillus cereus strain uw85 produces an antibiotic, designated zwittermicin a, that is associated with the ability of uw85 to suppress damping-off disease of alfalfa (medicago sativa) caused by the oomycete pathogen, phytophthora medicaginis, in a laboratory bioassay. we have identified certain culture conditions that promote or suppress zwittermicin a accumulation by uw85. maximum accumulation was detected in supernatants of trypticase soy broth cultures after sporulation, which is when culture ... | 1995 | 7546606 |
| prevalence of bacillus cereus in selected foods and detection of enterotoxin using tecra-via and bcet-rpla. | enterotoxigenic bacillus cereus was detected in cooked foods (17), rice noodles (3), wet wheat noodles (2), dry wheat noodles (10), spices (8), grains (4), legumes (11) and legume products (3). one hundred ninety-four (42.3%), 70 (15.3%) and 23 (5.2%) of the 459 presumptive b. cereus colonies isolated from pemba agar were identified as b. cereus, bacillus thuringiensis and b. mycoides, respectively. b. cereus isolates were examined for growth temperature, ph profile and enterotoxin production us ... | 1995 | 7547144 |
| biosynthesis and characterization of hydroxybutyrate-hydroxycaproate copolymers. | most polyhydroxyalkanoates (phas) reported to date fall into one of two broad classes: either hydroxybutyrate-hydroxyvalerate copolymers (typified by the pha produced by alcaligenes eutrophus), or hydroxyoctanoate-rich heteropolymers (typified by the pha produced by pseudomonas oleovorans). few reports of copolymers rich in hydroxybutyrate (hb), but containing a minor proportion of a co-monomer with a higher carbon number than valerate, have appeared. here we report on the biosynthesis and chara ... | 1995 | 7547720 |
| effects of three microbial probiotics on postprandial porto-arterial concentration differences of glucose, galactose and amino-nitrogen in the young pig. | postprandial kinetics of porto-arterial concentration differences of glucose (g), galactose (gal), l-lactic acid (la) and amino-n (an) were studied in the piglet after the ingestion of 10(7) colony-forming units (cfu) sporolactobacillus p44 (sp), or 10(6) cfu bacillus cereus ip5832 (ac), or 10(6) cfu of a combination of lactobacillus acidophilus, l. fermentum and l. brevis (ab)/g feed. sixteen fistulated piglets (portal vein and brachiocephalic trunk; mean body weight 22 (sd 2) kg) were used. th ... | 1995 | 7547825 |
| relation between micellar structure of model bile and activity of esterase. | in a model bile solution composed of lecithin (l)-bile salt (b), the solubilization of lipid and the accessibility of enzyme to the lipid were examined by observation of epr spectra and measurement of enzyme activity. the lifetime of the spin probe in the micellar phase was estimated to be approx. 1 microsecond by means of line shape analysis. both population and lifetime increased with temperature and the molar ratio of lecithin to bile salt (l/b). the epr data indicated that simple micelle of ... | 1995 | 7548175 |
| emesis of rhesus monkeys induced by intragastric administration with the hep-2 vacuolation factor (cereulide) produced by bacillus cereus. | to study the correlation between emetic toxin and hep-2 vacuole activity produced by bacillus cereus isolated from an outbreak of vomiting-type food poisoning, some properties and emetic activities of both purified hep-2 factor (cereulide) and partially purified factor to rhesus monkeys were determined. the results indicate that both cereulide and partially purified factor were very stable to digestion with proteolytic enzymes, different ph, and heating. vomiting was induced in the rhesus monkey ... | 1995 | 7557302 |
| [the microorganism count on impression materials following disinfection by dynamic plasma treatment]. | b. subtilis and b. cereus in concentrations under 10(5) mt, and of e. coli m-17 below 10(7) mt on different plaster materials die within fractures of a second after dynamic plasma processing at atmospheric pressure and at least one passing of the processed surface at a rate of 2.0 to 2.5 m/s at plasma flow power of 60 kw. | 1995 | 7570700 |
| interactions of biapenem with active-site serine and metallo-beta-lactamases. | biapenem, formerly ljc 10,627 or l-627, a carbapenem antibiotic, was studied in its interactions with 12 beta-lactamases belonging to the four molecular classes proposed by r. p. ambler (philos. trans. r. soc. lond. biol. sci. 289:321-331, 1980). kinetic parameters were determined. biapenem was readily inactivated by metallo-beta-lactamases but behaved as a transient inhibitor of the active-site serine enzymes tested, although with different acylation efficiency values. class a and class d beta- ... | 1995 | 7574520 |
| antimicrobial activity of cefotaxime tested against infrequently isolated pathogenic species (unusual pathogens). | the cefotaxime sodium spectrum of activity is very broad and includes many common species and a variety of less frequently isolated pathogens. we have reviewed the clinical microbiology literature (44 references) and the data base of the university of iowa hospitals and clinics (iowa city, ia) to collect data on the activity of cefotaxime against the less common species. cefotaxime was consistently active against actinobacillus actinomycetemcomitans, capnocytophaga spp., eikenella corrodens, ery ... | 1995 | 7587049 |
| the 3-d structure of a zinc metallo-beta-lactamase from bacillus cereus reveals a new type of protein fold. | the 3-d structure of bacillus cereus (569/h/9) beta-lactamase (ec 3.5.2.6), which catalyses the hydrolysis of nearly all beta-lactams, has been solved at 2.5 a resolution by the multiple isomorphous replacement method, with density modification and phase combination, from crystals of the native protein and of a specially designed mutant (t97c). the current model includes 212 of the 227 amino acid residues, the zinc ion and 10 water molecules. the protein is folded into a beta beta sandwich with ... | 1995 | 7588620 |
| enterotoxic activity of hemolysin bl from bacillus cereus. | bacillus cereus causes exotoxin-mediated diarrheal food poisoning. hemolysin bl (hbl) is a well-characterized b. cereus toxin composed of three components (b, l1, and l2) that together possess hemolytic, cytotoxic, dermonecrotic, and vascular permeability activities. here, we show that hbl causes fluid accumulation in ligated rabbit ileal loops at a dose of 5 micrograms of each component per loop. maximal fluid responses occurred for combinations of all three components at > or = 25 micrograms o ... | 1995 | 7591080 |
| antimicrobial activities of indolocarbazole and bis-indole protein kinase c inhibitors. ii. substitution on maleimide nitrogen with functional groups bearing a labile hydrogen. | new compounds, structurally related to the potent protein kinase c inhibitor staurosporine, and substituted on the imide nitrogen with a functional group bearing a labile hydrogen (hydroxymethyl, amino, hydroxy), were synthesized. their in vitro inhibitory potencies towards protein kinase c and protein kinase a showed that n-hydroxymethyl and n-hydroxy substitution, unlike alkyl substitution, can provide efficient protein kinase c inhibitors. the antimicrobial activities of these new compounds a ... | 1995 | 7592032 |
| phospholipid degradation in hypoxic/reoxygenated cardiomyocytes in response to phospholipase c from bacillus cereus. | in the present study, we investigated possible mechanisms behind exogenous phospholipase c-induced glycerol production in irreversibly damaged myocytes. rat ventricular myocytes were preincubated for 60 min in substrate-free krebs-henseleit bicarbonate buffer equilibrated with 95% n2-5% co2 (37 degrees c, ph = 7.4), resulting in exhaustion of cellular high energy phosphates and loss of rod-shaped morphology. at the end of the preincubation period, the incubation vials were divided into two group ... | 1995 | 7602607 |
| synthesis and evaluation of a non-radioactive gene probe for the detection of c.perfringens alpha toxin. | the synthesis and evaluation of a non-radioactive hybridization probe is described, specific detecting the clostridium perfringens alphatoxin gene (plc) by colony blot hybridization assay. a vector free digoxigenin-dutp-labelled probe was generated by polymerase chain reaction (pcr) targeting the cloned plc gene of c.perfringens strain atcc 13124. in a colony blot hybridization assay 296 strains of c.perfringens were tested for plc. none of the strains failed in hybridization. presence of plc wa ... | 1995 | 7603469 |
| topological properties of two cubic phases of a phospholipid:cholesterol:diacylglycerol aqueous system and their possible implications in the phospholipase c-induced liposome fusion. | water dispersions of phospholipid:cholesterol:diacylglycerol may, under certain conditions, originate either the lipid- and water-permeable q224 cubic phase, or the lipid-permeable but water-impermeable q227 cubic phase. these results are discussed within the framework of the phospholipase c-induced fusion of liposomes [nieva et al. (1993) biochemistry 32, 1054]. it is suggested that the cubic phases q224 and q227 represent two classes of lipid organization, one promoting, the other hindering th ... | 1995 | 7615069 |
| an antibacterial vitamin e derivative from tovomitopsis psychotriifolia. | the crude ethanol extract from the leaves of tovomitopsis psychotriifolia (clusiaceae) exhibits antibacterial activity against bacillus cereus, staphylococcus aureus, and pseudomonas aeruginosa. the biologically active agent in the extract has been isolated by chromatographic techniques and identified by nmr spectroscopy as trans-delta-tocotrienoloic acid. | 1995 | 7617773 |
| denitration of glycerol trinitrate by resting cells and cell extracts of bacillus thuringiensis/cereus and enterobacter agglomerans. | a number of microorganisms were selected from soil and sediment samples which were known to have been previously exposed to nitrate ester contaminants. the two most effective bacteria for transforming glycerol trinitrate (gtn) were identified as bacillus thuringiensis/cereus and enterobacter agglomerans. for both isolates, denitration activities were expressed constitutively and gtn was not required for induction. dialysis of cell extracts from both isolates did not affect denitration, which ind ... | 1995 | 7618866 |
| screening and characterization of microorganisms with glutaryl-7adca acylase activity. | a screening of microorganisms producing glutaryl-7adca acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7adca), has been carried out in soil samples. five microorganisms expressing acylase activity were isolated and classified as bacillus cereus, achromobacter xylosooxidans, bacillus sp., pseudomonas sp. and pseudomonas paucimobilis. the screening was carried out by preparing enrichment cultures containing glutaryl-7-a ... | 1995 | 7632401 |
| mutation in aspartic acid residues modifies catalytic and haemolytic activities of bacillus cereus sphingomyelinase. | four aspartic acid residues (asp126, asp156, asp233 and asp295) of bacillus cereus sphingomyelinase (smase) in the conservative regions were changed to glycine by in vitro mutagenesis, and the mutant smases [d126g (asp126-->gly etc.), d156g, d233g and d295g] were produced in bacillus brevis 47, a protein-producing strain. the sphingomyelin (sm)-hydrolysing activity of d295g was completely abolished and those of d126g and d156g were reduced by more than 80%, whereas that of d233g was not so profo ... | 1995 | 7639690 |
| bacillus cereus and bacillus thuringiensis isolated in a gastroenteritis outbreak investigation. | during investigation of a gastroenteritis outbreak in a chronic care institution, norwalk virus was found in stool specimens from two individuals and bacterial isolates presumptively identified as bacillus cereus were isolated from four individuals (including one with norwalk virus) and spice. phage typing confirmed all bacillus clinical isolates were phage type 2. all clinical isolates were subsequently identified as b. thuringiensis when tested as a result of a related study (l. leroux, person ... | 1995 | 7639990 |
| a survey of enteropathogens among united states military personnel during operation bright star '94, in cairo, egypt. | acute gastroenteritis is a potential cause of substantial morbidity in u.s. military personnel during deployment. this study was conducted to evaluate enteric pathogens associated with diarrhea in a u.s. military population on deployment in cairo, egypt, during november 1993. enteric pathogens found to be associated with cases of diarrhea included: enterotoxigenic escherichia coli (etec), 27% (22% heat-stable [st], 3% heat-labile [lt], and 2% st/lt producers); campylobacter spp., 3%; and salmone ... | 1995 | 7659237 |
| fatal bacillus cereus meningoencephalitis in an adult with acute myelogenous leukemia. | bacillus cereus, a ubiquitous, endospore-forming, aerobic gram-positive bacillus, is primarily associated with toxin-mediated food poisoning. frequently, isolates of bacillus species from clinical specimens are discussed as contaminants. we report a rapidly fatal case of disseminated infection due to b cereus in a patient receiving induction chemotherapy for m0 acute leukemia. a short clinical syndrome of nausea and vomiting preceded neurologic symptoms. autopsy showed extensive meningoencephali ... | 1995 | 7660218 |
| crystal structure of the phosphatidylinositol-specific phospholipase c from bacillus cereus in complex with myo-inositol. | phosphatidylinositol (pi), once regarded as an obscure component of membranes, is now recognized as an important reservoir of second messenger precursors and as an anchor for membrane enzymes. pi-specific phospholipase c (pi-plc) is the enzyme that cleaves pi, invoking numerous cellular responses. the crystal structure of pi-plc from bacillus cereus (ec 3.1.4.10) has been solved at 2.6 a resolution and refined to a crystallographic r factor of 18.7%. the structure consists of an imperfect (beta ... | 1995 | 7664726 |
| genetic variability of bacillus anthracis and related species. | we evaluated the abilities of pulsed-field gel electrophoresis (pfge) and sequences of intergenic spacer regions (isrs) between two highly conserved genes, 16s-23s rdna and gyrb-gyra isrs, to detect variation in strains of bacillus anthracis as well as two closely related species, b. cereus atcc 14579 and b. mycoides atcc 6462. for each restriction enzyme, (noti, sfii, and smai), the pfge banding patterns for three b. anthracis strains (ames, vollum, and sterne) were identical. however, closely ... | 1995 | 7665658 |
| evidence for a bifurcation of the mitogenic signaling pathway activated by ras and phosphatidylcholine-hydrolyzing phospholipase c. | nih 3t3 cells stably transfected with the gene encoding phosphatidylcholine-hydrolyzing phospholipase c (pc-plc) from bacillus cereus display a chronic elevation of intracellular diacylglycerol levels and a transformed phenotype. we have used such pc-plc-transformed cells to evaluate the roles of the cytoplasmic serine/threonine kinases raf-1, zeta protein kinase c (zeta pkc) and protein kinase a (pka) in oncogenesis and mitogenic signal transduction elicited by phosphatidylcholine hydrolysis. w ... | 1995 | 7673165 |
| phase partitioning detects differences between phospholipase-released forms of alkaline phosphatase--a gpi-linked protein. | a number of enzymes are known to release alkaline phosphatase and other glycan phosphatidylinositol-anchored proteins from membrane surfaces. we describe a novel approach to detect and measure these activities by partitioning in aqueous phase systems. the procedures avoid the complications of micelle-formation involving hydrophobic molecules that may arise with detergent-based partition systems and can clearly distinguish between inositol-specific phospholipase c and d activities. | 1993 | 7678987 |
| protein kinase c zeta isoform is critical for mitogenic signal transduction. | the requirement of protein kinase c zeta (zeta pkc) for maturation of x. laevis oocytes in response to insulin, p21ras, and phosphatidylcholine-hydrolyzing phospholipase c has recently been shown. here we present experimental evidence demonstrating that activation of zeta pkc is not only necessary but also sufficient by itself to activate maturation in oocytes and to produce deregulation of growth control in mouse fibroblasts. furthermore, by using a dominant kinase-defective mutant of zeta pkc, ... | 1993 | 7688666 |
| sensitivity of phospholipase c (bacillus cereus) activity to lipid packing in sonicated lipid mixtures. | we have recently demonstrated that phospholipase c (plc) activity on membranes decreases in the presence of membrane-active peptides such as alamethicin, gramicidin s, and melittin [rao, n. m. (1992) biochem. biophys. res. commun. 182, 682-688]. since these peptides affect lipid packing in the membrane and induce nonbilayer phases depending on the lipid composition, we tested for the sensitivity of plc activity to lipid packing. we monitored plc activities on four lipid systems which demonstrate ... | 1993 | 7689334 |
| analysis of a common antigenic epitope in bacillus cereus flagellar fraction. | the strain-specific antigenicity between bacillus cereus decreased but the common antigenicity did not change, when the flagellar antigen was treated with ph 2. flagellar antigen of b. cereus h.1 was separated into two fractions by gel chromatography, one with a specific antigenicity (p1 fraction), and the other with a common antigenicity (p2 fraction). it was revealed that a common antigenic epitope of the p2 fraction was detected on a 61 kda protein by further purification using a mono q colum ... | 1993 | 7689894 |
| molecular cloning and characterization of the hbla gene encoding the b component of hemolysin bl from bacillus cereus. | previous evidence suggests that hemolysin bl, which consists of a binding component, b, and two lytic components, l1 and l2, is the enterotoxin responsible for the diarrheal form of gastroenteritis caused by food-borne strains of bacillus cereus. to prove that hemolysin bl and the enterotoxin are the same requires large amounts of these components free of other b. cereus proteins. for this purpose, we sought to clone the gene encoding the b component and to express it in escherichia coli. a part ... | 1993 | 7693651 |
| kinetic analysis of extension of substrate specificity with xanthomonas maltophilia, aeromonas hydrophila, and bacillus cereus metallo-beta-lactamases. | twenty beta-lactam molecules, including penicillins, cephalosporins, penems, carbapenems, and monobactams, were investigated as potential substrates for xanthomonas maltophilia ula-511, aeromonas hydrophila ae036, and bacillus cereus 5/b/6 metallo-beta-lactamases. a detailed analysis of the kinetic parameters examined confirmed these enzymes to be broad-spectrum beta-lactamases with different ranges of catalytic efficiency. cefoxitin and moxalactam, substrates for the beta-lactamases from x. mal ... | 1995 | 7695305 |
| studies of one invasive and two noninvasive methods for detection of bacterial contamination of platelet concentrates. | recently, a new method for noninvasive detection of bacterial contamination of platelet concentrates was described [arpi et al.: vox sang 1993;65:335-336]. a co2-sensitive label is applied on the outer surface of the plastic container of the platelet concentrate. when any contaminating bacteria have increased the pco2 to a certain level the label changes color, indicating bacterial growth. we have studied this technique and applied such sensitive labels both directly onto pcs and onto plastic ba ... | 1994 | 7701805 |
| growth and survival of yersinia enterocolitica, salmonella and bacillus cereus in brie stored at 4, 8 and 20 degrees c. | the potential of stabilised brie to support growth of the food-borne pathogens, yersinia enterocolitica serotypes 0:3 and 0:9, salmonella typhimurium, s. dublin (both dairy isolates), s. thompson, and bacillus cereus (3 dairy isolates), after contamination on opening the cheese package was evaluated. growth kinetics of the different pathogens was determined in relation to inoculum size and storage temperature (4 degrees c, 8 degrees c and 20 degrees c). only y. enterocolitica was found to grow o ... | 1994 | 7703008 |
| antimicrobial mode of action of secretions from the metapleural gland of myrmecia gulosa (australian bull ant). | secretions from exocrine metapleural glands of myrmecia gulosa (australian bull ant) exhibit broad-spectrum antimicrobial activity. treatment of the yeast candida albicans with metapleural secretion resulted in the rapid and total leakage of k+ ions from cells within 10 min. ultrastructural analysis of the bacteria bacillus cereus, escherichia coli, and pseudomonas aeruginosa, and cells and protoplasts of candida albicans demonstrated gross damage of the cell membrane and aggregation of the cyto ... | 1995 | 7720011 |
| cephalosporin derivatives of doxorubicin as prodrugs for activation by monoclonal antibody-beta-lactamase conjugates. | the synthesis of a series of cephalosporin doxorubicin derivatives that differ with respect to the substituent at position 7 of the cephem nucleus is described. these compounds are designed as prodrugs of doxorubicin for activation by monoclonal antibody-beta-lactamase conjugates. the key step in the synthesis of this series of compounds involves the use of the phenylacetamido group as an enzymatically removable protecting group for the 7-amino group on the cephem. in vitro cytotoxicity assays w ... | 1995 | 7731023 |
| inactivation of blasticidin s by bacillus cereus. v. purification and characterization of blasticidin s-deaminase mediated by a plasmid from blasticidin s resistant bacillus cereus k55-s1. | blasticidin s (bs) deaminase (bsr) from a bs-resistant strain, bacillus cereus k55-s1, was purified to homogeneity. molecular weights determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and by gel filtration on hplc are about 15500 and 35000, respectively, indicating the enzyme is a homodimer. the amino acid composition and n-terminal sequence of bsr are the same as those deduced from the nucleotide sequence of the bs-resistant gene, bsr. the optimum temperature an ... | 1995 | 7742811 |
| locating nisin-producing lactococcus lactis in a fermented meat system. | antibody-linked probes were used to locate nisin in a fermented meat system. free nisin or nisin bound to susceptible cells or food components was not detected. colonies of nisin-producing lactococcus lactis were stained at all times during growth. the position of nisin-producing l. lactis colonies was noted and compared with the location of spoilage organisms or the distribution of areas with a fermented meat appearance. no relationship between the distribution of starter culture and the locati ... | 1995 | 7744718 |
| differentiation of bacillus anthracis and other 'bacillus cereus group' bacteria using is231-derived sequences. | sequences based on the conserved 20 bp inverted repeat of is231 variants were used as polymerase chain reaction-based fingerprinting primers of the member species of the bacillus cereus group (b. anthracis, b. cereus, b. thuringiensis and b. mycoides), because of their close association with transposons, principally tn4430 in b. thuringiensis. fingerprints of b. anthracis were simple, and specifically allowed its identification and sub-differentiation from other members of the group. fingerprint ... | 1995 | 7750728 |
| bacillus cereus phage typing as an epidemiological tool in outbreaks of food poisoning. | bacillus cereus is responsible for an increasing number of food poisoning cases. by using 12 bacteriophages isolated from sewage, a typing scheme for b. cereus isolates from outbreaks or sporadic cases of food poisoning was developed. the phages belonged to three morphotypes. ten phages with contractile tails and icosahedral heads were members of the myoviridae family, and two phages with noncontractile tails belonged to the siphoviridae family. phage 11 represented a new species. it had an isom ... | 1995 | 7751369 |
| the effect of recovery conditions on the apparent heat resistance of bacillus cereus spores. | the effect of recovery media and incubation temperature on the apparent heat resistance of three atcc strains (4342, 7004 and 9818) of bacillus cereus spores were studied. nutrient agar (na), tryptic soy agar (tsa), plate count agar (pca) and milk agar (ma) as the media and temperatures in the range of 15-40 degrees c were used to recover heated spores. higher counts of heat injured spores were obtained on pca and na. the optimum subculture temperature was about 5 degrees c below the optimum tem ... | 1995 | 7759384 |
| anti-bacterial antibodies in epstein-barr virus (ebv)-transformed oligoclonal b-cell lines established from normal persons and autoimmune disease patients. | we have established 950 and 430 oligoclonal b-lymphoblastoid cell lines (lcl) from two normal persons and eight autoimmune disease patients, respectively by using epstein-barr virus (ebv)-induced transformation. to re-evaluate the ebv technique for production of human monoclonal antibodies (mab) related to infectious disease, we screened these oligoclonal lcls for antibodies against 31 bacterial strains systematically. a total of 74 cultures out of 1380 were reactive to a total of 18 strains out ... | 1994 | 7765395 |
| comparison of the tecra via kit, oxoid bcet-rpla kit and cho cell culture assay for the detection of bacillus cereus diarrhoeal enterotoxin. | two commercial serological kits (oxoid bcet-rpla and tecra via) and a chinese hamster ovary (cho) cell cytotonicity assay for the detection of bacillus cereus diarrhoeal enterotoxin were compared. eleven b. cereus strains and one enterotoxigenic b. thuringiensis strain were evaluated. both kits and the cho cell assay yielded positive toxin responses for cell-free culture filtrates from eight out of 11 diarrhoeal enterotoxigenic strains. an emetic enterotoxin producing strain was negative with al ... | 1994 | 7765448 |
| identification of dna-binding proteins changed after induction of sporulation in bacillus cereus. | dna-binding proteins were extracted from both exponentially growing cells of bacillus cereus ts-4 and cells that were induced to sporulate at different stages of chromosome replication, by using a double-stranded b. cereus ts-4 dna-cellulose column. two-dimensional electrophoresis of the proteins found that the amounts of 17 proteins changed drastically after induction of sporulation at all the stages. for 8 of those proteins, the largest or the smallest amount was found in the cells which were ... | 1995 | 7766022 |
| oleic acid transformations by selected strains of sphingobacterium thalpophilum and bacillus cereus from composted manure. | in a survey of 186 randomly selected microbial strains isolated from composted manure, 63 transformed oleic acid into three types of products: hydroxy fatty acid, fatty amide, and less polar oleyl lipid. selection of oleic acid-transforming microorganisms was enhanced in nutrient agar supplemented with 0.1% (vol/vol) oleic acid at ph 7.2. most of the 63 diverse isolates elicited inconsistent and poorly reproduced transformations. however, strains 142b (nrrl b-14797) transformed oleic acid to 10- ... | 1995 | 7767231 |
| the bcet gene of bacillus cereus encodes an enterotoxic protein. | a toxin gene (bcet) on a 2.9 kb dna fragment of bacillus cereus b-4ac was cloned and expressed in escherichia coli, and its nucleotide sequence determined. the dna fragment contained an open reading frame capable of encoding a polypeptide of 336 amino acids with a molecular mass of 41039 da. the translated product in e. coli exhibited vero cell cytotoxicity, and was positive in a vascular permeability assay. it also caused fluid accumulation in a ligated mouse ileal loop and was lethal to mice u ... | 1995 | 7773399 |
| a novel dodecadepsipeptide, cereulide, is an emetic toxin of bacillus cereus. | a vacuole-formation substance, cereulide of bacillus cereus, is an emetic toxin in animals. both oral administration and intraperitoneal injection of cereulide caused dose-dependent emesis in suncus murinus, a new animal model of emesis. vagotomy or a 5-ht3 receptor antagonist completely abolished this emetic effect. therefore, cereulide causes emesis through the 5-ht3 receptor and stimulation of the vagus afferent. we also found that our purified cereulide caused swelling of mitochondria of hep ... | 1995 | 7781985 |
| postmortem drug metabolism by bacteria. | studies were undertaken to determine the possible role of enteric bacteria in the postmortem bioconversion of the nitrobenzodiazepines flunitrazepam, clonazepam, and nitrazepam. flunitrazepam, clonazepam, and nitrazepam were completely metabolized in blood in the presence of eight species of enteric bacteria to their respective 7-amino-metabolites. the rates of metabolism, at 37 degrees c, ranged from 0.1 ng/ml/min for streptococcus faecalis to 8.8 ng/ml/min for clostridium perfringens. the rate ... | 1995 | 7782744 |
| analysis of dipalmitoyl phosphatidylcholine in amniotic fluid by enzymatic hydrolysis and high-performance thin-layer chromatography reflectance spectrodensitometry. | a novel test for the determination of dipalmitoyl phosphatidylcholine (dppc) in amniotic fluid (af) as free dipalmitoylglycerol (dpg), is described. aliquots of amniotic fluid were hydrolyzed with bacilus cereus phospholipase c, and the resulting diglycerides analyzed by agno3-modified high-performance thin-layer chromatography (hptlc)-reflectance spectrodensitometry. this hptlc system provided resolution of dpg and palmitoylpalmitoleoylglycerol (pog) from other 1,2-diglycerides and cholesterol. ... | 1995 | 7795804 |
| zwittermicin a-producing strains of bacillus cereus from diverse soils. | bacillus cereus uw85 produces a novel aminopolyol antibiotic, zwittermicin a, that contributes to the ability of uw85 to suppress damping-off of alfalfa caused by phytophthora medicaginis. uw85 produces a second antibiotic, provisionally designated antibiotic b, which also contributes to suppression of damping-off but has not been structurally defined yet and is less potent than zwittermicin a. the purpose of this study was to isolate genetically diverse strains of b. cereus that produce zwitter ... | 1994 | 7811080 |
| identification and analysis of the antigens detected by two commercial bacillus cereus diarrheal enterotoxin immunoassay kits. | the usefulness of two commercial immunoassays for the detection of diarrheal enterotoxin of bacillus cereus is unclear because the identity of the enterotoxin(s) has not been proven and the kits detect different proteins. we found that the bacillus cereus enterotoxin-reversed passive latex agglutination kit (oxoid) detects the l2 component from hemolysin bl, and the bacillus diarrhoeal enterotoxin visual immunoassay (tecra) detects two apparently nontoxic proteins. | 1994 | 7811099 |
| bacillus cereus in infant foods and dried milk products. | dried milk products and infant food are known to be frequently contaminated with bacillus cereus. sources of the organism and its behaviour in the product and in the equipment during processing are discussed. with regard to the incidence of b. cereus in infant food, 261 samples distributed in 17 countries were collected and examined for its b. cereus content. fifty-four percent of the samples were contaminated with b. cereus reaching levels from 0.3 to 600/g. counts higher than 10/g were found i ... | 1994 | 7811567 |
| stability of spores of bacillus cereus stored on silicagel. | spores of four different strains of bacillus cereus were stored on silicagel at 22 degrees c and in physiological saline solution at -20 degrees c for a period of 260 days. at different intervals the spores were tested for survival, heat sensitivity and capacity to germinate. there was no clear change in any of the parameters tested, so storage on silicagel can be a good alternative for storage as a frozen suspension. spores stored in this way can easily be exchanged for reference material and u ... | 1994 | 7811568 |
| characteristics of bacillus cereus related to safe food production. | thirty bacillus cereus strains, isolated from different sources, were characterized in relation to safe food production. the minimal growth temperatures of the strains varied from < or = 5 degrees c to 11 degrees c. generation times at 7 degrees c of strains capable of growing at temperatures < or = 5 degrees c were approximately 8.2 h. the d90 degrees c-values of spores of strains with a minimal growth temperature of 11 degrees c determined in phosphate buffer at ph 7.0 ranged from 4.8 to > 200 ... | 1994 | 7811576 |
| rho-adp-ribosylating exoenzyme from bacillus cereus. purification, characterization, and identification of the nad-binding site. | the adp-ribosyltransferase produced by a pathogenic strain of bacillus cereus was purified to near homogeneity. the transferase is a 28,000 da molecular mass enzyme with a pi of 10.3. the specific enzyme activity is 7.0 nmol of adp-ribose min-1 mg-1 with a km for nad of 0.3 microm. partial amino acid sequence analysis of the exoenzyme reveals no significant homology to clostridium botulinum c3 nor to clostridium limosum exoenzyme. the novel exoenzyme selectively modifies the small gtp-binding pr ... | 1995 | 7819216 |
| phospholipase-c-promoted liposome fusion. | 1994 | 7821697 | |
| extracellular virulence factors in bacillus cereus endophthalmitis: methods and implication of involvement of hemolysin bl. | bacillus cereus is a common cause of highly fulminant posttraumatic and metastatic endophthalmitis. exotoxins or enzymes likely contribute to the severity of the infection, but specific virulence factors have not been identified. we developed two methods for the identification of b. cereus ocular virulence factors. in an in vitro assay that allows screening of multiple samples, retinal toxicity was estimated by measuring the release of lactate dehydrogenase from retinal buttons treated with b. c ... | 1995 | 7822032 |