Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| genetic characteristics and pathogenicity of human hepatitis e virus in nanjing, china. | to investigate the genetic characteristics and pathogenicity of hepatitis e virus (hev) and assess the potential risk factors for sporadic hepatitis e. | 2012 | 22408357 |
| serological and molecular study of hepatitis e virus among illegal blood donors. | to investigate the seroprevalence and molecular characteristics of hepatitis e virus (hev) in the illegal blood donors (ibds) of central china in the early 1990s. | 2012 | 22408360 |
| low risk of hepatitis e virus reactivation after haematopoietic stem cell transplantation. | hepatitis e virus (hev) is an emerging cause of acute hepatitis in industrialized countries. hev infection can evolve to chronic hepatitis in immunocompromised patients. additionally, hev reactivation after haematopoietic stem cell transplantation has been reported. | 2012 | 22425538 |
| detection of human food-borne and zoonotic viruses on irrigated, field-grown strawberries. | this study evaluated the presence of pathogenic human and zoonotic viruses on irrigated, field-grown strawberries. norovirus genogroup i, rotavirus, and swine hepatitis e virus genogroup 3 were detected on strawberries, and irrigation water is suspected as the contamination origin. | 2012 | 22427499 |
| hepatitis e virus replication requires an active ubiquitin-proteasome system. | the mechanism of hepatitis e virus (hev) replication remains largely unknown. here we demonstrate that hev replication requires an active ubiquitin-proteasome system and that proteasome inhibitors affect hev replication, possibly by inhibition of viral transcription or/and translation without a significant effect on cellular translation. overexpression of ubiquitin in inhibitor-treated cells partially reverses the inhibitor effect on hev replication. the results suggest that hev replication requ ... | 2012 | 22438536 |
| the psap motif within the orf3 protein of an avian strain of the hepatitis e virus is not critical for viral infectivity in vivo but plays a role in virus release. | the orf3 protein of hepatitis e virus (hev) is a multifunctional protein important for virus replication. the orf3 proteins from human, swine, and avian strains of hev contain a conserved pxxp amino acid motif, resembling either src homology 3 (sh3) cell signaling interaction motifs or "late domains" involved in host cell interactions aiding in particle release. using an avian strain of hev, we determined the roles of the conserved prolines within the prepsapp motif in hev replication and infect ... | 2012 | 22438540 |
| hepatitis e virus-associated aplastic anaemia: the first case of its kind. | here we report the case of a 32-year-old pakistani male, who developed severe aplastic anaemia after a severe attack of acute hepatitis e virus (hev) infection. his laboratory test values were not in normal ranges. the liver enzymes were elevated. serologic and/or molecular-based tests for hepatitis a, b, c, d, g, transfusion transmitted virus (ttv) and b19 were negative, whereas anti-hev igm and hev rna polymerase chain reaction (pcr) was also detected in the patient sample. the patient receive ... | 2012 | 22441030 |
| identical consensus sequence and conserved genomic polymorphism of hepatitis e virus during controlled interspecies transmission. | high-throughput sequencing of bile and feces from two pigs experimentally infected with human hepatitis e virus (hev) of genotype 3f revealed the same full-length consensus sequence as in the human sample. twenty-nine percent of polymorphic sites found in hev from the human sample were conserved throughout the infection of the heterologous host. the interspecies transmission of hev quasispecies is the result of a genomic negative-selection pressure on random mutations which can be deleterious to ... | 2012 | 22457521 |
| prevalence of hepatitis e virus in swine fed on kitchen residue. | the aim of this study was to investigate the prevalence of swine hepatitis e virus (hev) in pigs fed different feedstuffs (kitchen residue or mixed feeds) and genetic identification of hev isolated in hebei province, china. serum and fecal samples were collected from adult swine. anti-hev antibody was evaluated by double sandwich antigen enzyme immunoassay. hev rna was extracted from fecal samples and amplified by nested rt-pcr. the reaction products were sequenced, and the sequence analyzed. vi ... | 2012 | 22457765 |
| hepatitis e: a complex and global disease. | thirty years after its discovery, the hepatitis e virus (hev) continues to represent a major public health problem in developing countries. in developed countries, it has emerged as a significant cause of non-travel-associated acute hepatitis. hev infects a wide range of mammalian species and a key reservoir worldwide appears to be swine. genomic sequence similarity between some human hev genotypes and swine hev strains has been identified and we know that humans can acquire hev infection from a ... | 2008 | 22460217 |
| prevalence of hepatitis e virus antibodies in individuals exposed to swine in mato grosso, brazil. | this study was conducted to estimate the prevalence of hepatitis e antibodies (anti-hev) among individuals exposed to swine in the rural areas of the state of mato grosso (mt) in brazil. the study included 310 participants who had an average age of 39 years. fifty-one per cent of the participants were female and 26 (8.4%) were anti-hev-positive. concomitantly, we studied 101 blood donors from the urban area of the state capital who had never lived in a rural area or handled swine. four per cent ... | 2012 | 22510828 |
| hepatitis e virus infection among solid organ transplant recipients, the netherlands. | we screened 1,200 living heart, lung, liver, and kidney transplant recipients for hepatitis e virus infection by reverse transcription pcr. in 12 (1%) patients, hepatitis e virus infection was identified; in 11 patients, chronic infection developed. this immunocompromised population is at risk for hepatitis e virus infection. | 2012 | 22516170 |
| hepatitis a, b, c and e virus markers in chinese residing in tokyo, japan. | recently, the number of foreigners living in japan has been increasing, with the majority originating from china. it is important for us to know the prevalence of hepatitis virus markers among them, as proper medical practices and vaccinations should be prepared when seeing them and their offspring. | 2012 | 22524475 |
| low risk of hepatitis e among dutch short-term travelers. | hepatitis e is endemic in (sub)tropical countries while only sporadic cases have been described in industrialized countries. in a prospective study among 1270 short-term dutch travelers to (sub)tropical countries we found no seroconversion to anti-hepatitis e virus (hev) antibodies, indicating a very low risk for travelers to acquire a hepatitis e infection. | 2012 | 22530832 |
| prevalence and transmission of hepatitis e virus in domestic swine populations in different european countries. | hepatitis e virus (hev) genotype 3 and 4 can cause liver disease in human and has its main reservoir in pigs. hev investigations in pigs worldwide have been performed but there is still a lack of information on the infection dynamics in pig populations. | 2012 | 22534364 |
| cross-species infection of pigs with a novel rabbit, but not rat, strain of hepatitis e virus isolated in the united states. | hepatitis e virus (hev) is an important human pathogen. in addition to humans, hev has also been identified in pig, chicken, mongoose, deer, rat, rabbit and fish. there are four recognized and two putative genotypes of mammalian hev. genotypes 1 and 2 are restricted to humans, while genotypes 3 and 4 are zoonotic. the recently identified rabbit hev is a distant member of genotype 3. here, we first expressed and purified the recombinant capsid protein of rabbit hev and showed that the capsid prot ... | 2012 | 22535776 |
| pathogenesis and treatment of hepatitis e virus infection. | hepatitis e has been considered to be a travel-associated, acute, self-limiting liver disease that causes fulminant hepatic failure in specific high-risk groups only. however, hepatitis e virus (hev) infection can also be acquired in industrialized countries-hev genotype 3 infection is a zoonosis, with pigs and rodents serving as animal reservoirs. in recent years, cases of chronic hev infection that were associated with progressive liver disease have been described in several cohorts of immunoc ... | 2012 | 22537448 |
| [hepatitis e. current perspectives]. | infection with hepatitis e virus (hev) is highly prevalent in developing countries and the who estimates one third of the world population has had contact with the virus. its diagnosis and epidemiology are well known in endemic countries but, recently, there have been sporadic cases in developed countries in patients with no history of travel. currently in these countries, hepatitis e is considered a zoonosis yet there remain to be known other routes of transmission. another interesting aspect i ... | 2012 | 22538059 |
| the hepatitis e virus polyproline region is involved in viral adaptation. | genomes of hepatitis e virus (hev), rubivirus and cutthroat virus (ctv) contain a region of high proline density and low amino acid (aa) complexity, named the polyproline region (ppr). in hev genotypes 1, 3 and 4, it is the only region within the non-structural open reading frame (orf1) with positive selection (4-10 codons with dn/ds>1). this region has the highest density of sites with homoplasy values >0.5. genotypes 3 and 4 show ∼3-fold increase in homoplastic density (hd) in the ppr compared ... | 2012 | 22545153 |
| multiplex real-time rt-pcr for detecting chikungunya virus and dengue virus. | to develop diagnostic test for detection chikungunya virus (chikv and dengue virus (denv) infection. | 2012 | 22546647 |
| hepatitis e virus: what transplant physicians should know. | hepatitis e virus (hev) infection is an underdiagnosed disease in the developed world. in pediatric and adult organ transplant patients hev infection can cause chronic hepatitis, which can lead to cirrhosis. extra-hepatic manifestations, such as neurological symptoms and kidney injury, have been also reported in transplant patients. in this comprehensive minireview, we summarize the current knowledge on hev infection in transplant patients, that is, its prevalence, incidence, natural history and ... | 2012 | 22549004 |
| hepatitis e. | hepatitis e virus (hev) was discovered during the soviet occupation of afghanistan in the 1980s, after an outbreak of unexplained hepatitis at a military camp. a pooled faecal extract from affected soldiers was ingested by a member of the research team. he became sick, and the new virus (named hev), was detected in his stool by electron microscopy. subsequently, endemic hev has been identified in many resource-poor countries. globally, hev is the most common cause of acute viral hepatitis. the v ... | 2012 | 22549046 |
| rat hepatitis e virus: geographical clustering within germany and serological detection in wild norway rats (rattus norvegicus). | zoonotic hepatitis e virus (hev) infection in industrialised countries is thought to be caused by transmission from wild boar, domestic pig and deer as reservoir hosts. the detection of hev-specific antibodies in rats and other rodents has suggested that these animals may represent an additional source for hev transmission to human. recently, a novel hev (rathev) was detected in norway rats from hamburg, germany, showing the typical genome organisation but a high nucleotide and amino acid sequen ... | 2012 | 22554648 |
| three cases of acute or fulminant hepatitis e caused by ingestion of pork meat and entrails in hokkaido, japan: zoonotic food-borne transmission of hepatitis e virus and public health concerns. | in developed countries including japan, the transmission route of indigenous hepatitis e virus (hev) infection is obscure. accordingly, public health implications of indigenous hev infection have not been well addressed. the aim of this study was to clarify the route of transmission of a small outbreak of acute hepatitis e and assess the public health implications of indigenous zoonotic hev transmission. | 2012 | 22568494 |
| hepatitis e virus enters liver cells through receptor-dependent clathrin-mediated endocytosis. | we investigated the virus-host interaction for hepatitis e virus (hev) by performing competitive binding assays using in vitro assembled virus-like particles (vlps). we used escherichia coli expressed native capsid protein (porf2) and its mutants with an attached gly((5))-ala (linker) reporter [enhanced green fluorescent protein (egfp)/firefly luciferase (fluc)]. transmission electron microscopy and nanoparticle tracking showed near uniform particles of approximately 30-35 nm in diameter for por ... | 2011 | 22571906 |
| hev infection in swine from eastern brazilian amazon: evidence of co-infection by different subtypes. | hepatitis e virus (hev) is a fecal-orally transmitted member of the genus hepevirus that causes acute hepatitis in humans and is widely distributed throughout the world. pigs have been reported as the main source of genotypes 3 and 4 infection to humans in non-endemic areas. to investigate hev infection in pigs from different regions of pará state (eastern brazilian amazon), we performed serological and molecular analyses of serum, fecal and liver samples from 151 adult pigs slaughtered between ... | 2012 | 22579295 |
| detection and characterization of hepatitis e virus in domestic pigs of different ages in portugal. | this study represents the primary hepatitis e virus (hev) surveillance in domestic pigs in portugal, five pig farms were investigated in 5 different portuguese regions, ten faecal samples were collected at four different stages of the production. all faecal samples were tested for hepatitis e virus by real-time rt-pcr. at least one sample from each farms of all age groups tested positive for hev. the prevalence in the pig herds varied from 10% to 30% and the mean prevalence was 32% in weaners, 2 ... | 2012 | 22583975 |
| molecular characterization of hepatitis e virus in patients with acute hepatitis in venezuela. | hepatitis e virus (hev) causes a common infection in developing countries. hev infection occurs as outbreaks, as sporadic clinical cases and as large epidemics in endemic areas. the objective of this study was to determine the presence of hev infection in patients with clinical suspicion of hepatitis a virus (hav) infection, referred to the instituto nacional de higiene "rafael rangel" in venezuela. seventy-four sera were tested for anti-hav and anti-hev igm antibodies. hev-rna was amplified fro ... | 2012 | 22585718 |
| the orf2 glycoprotein of hepatitis e virus inhibits cellular nf-κb activity by blocking ubiquitination mediated proteasomal degradation of iκbα in human hepatoma cells. | nuclear factor kappa b (nf-κb) is a key transcription factor that plays a crucial role in host survival during infection by pathogens. therefore, it has been a priority of many pathogens to manipulate the cellular nf-κb activity in order to create a favorable environment for their survival inside the host. | 2012 | 22590978 |
| are hiv-infected patients a high-risk population for hepatitis e virus infection in spain? | 2012 | 22591605 | |
| hepatitis e infection is an under recognized cause of acute decompensation in patients with chronic liver disease. | we aimed to assess characteristics of patients with a positive hepatitis e virus serology with emphasis on acute on chronic liver disease. | 2012 | 22592074 |
| characterization of hepatitis e virus recombinant orf2 proteins expressed by vaccinia viruses. | hepatitis e virus (hev), an enterically transmitted pathogen, is one of the major causes of acute hepatitis in humans worldwide, being responsible for outbreaks and epidemics in regions with suboptimal sanitary conditions, in many of which it is endemic. in industrialized countries, hepatitis e is rarely reported, but recent studies have revealed quite high human seroprevalence rates and the possibility of porcine zoonotic transmission. there is currently no specific therapy or licensed vaccine ... | 2012 | 22593167 |
| acute hepatitis e virus infection and autoimmune thyroiditis: yet another trigger? | a middle aged woman, previously healthy with the exception of mild seasonal asthma was presented with signs of acute hepatitis. the further investigation showed acute hepatitis e virus infection associated with autoimmune thyroiditis. treatment was started with propranolol and carbimazol whereupon hepatitis and hyperthyroidism resolved. the authors think that the observed association of acute hepatitis e virus infection and autoimmune thyroiditis suggests a role of hepatitis e virus as putative ... | 2012 | 22604767 |
| hepatitis e virus infection in sheltered homeless persons, france. | 2012 | 22607775 | |
| [hepatitis e: scale of the problem in spain]. | hepatitis e virus (hev) infection is one of the most frequent causes of acute hepatitis worldwide. however, in spain, hev causes only a tiny number of cases of acute hepatitis, the most prevalent cause being hepatitis a. most cases of hev in spain are "imported", being acquired through travel to areas where this infection is endemic. nevertheless, in the last few years a growing number of "autochthonous cases" have been reported in persons with no history of travelling to hev-endemic areas. the ... | 2012 | 22608490 |
| thermal inactivation of infectious hepatitis e virus in experimentally contaminated food. | hepatitis e virus (hev) infection of zoonotic origin is an emerging concern in industrialized countries. in the past few years, several cases of zoonotic hepatitis e have been identified and the consumption of food products derived from pork liver have been associated with clusters of human cases. more specifically, raw or undercooked pork products have been incriminated. few data on the effect of heating on hev inactivation in food products are available. in the present study, the various times ... | 2012 | 22610436 |
| hepatitis e virus as a causative agent of unexplained liver enzyme elevations in hiv-infected patients. | 2012 | 22622079 | |
| duplex rt-qpcr for the detection of hepatitis e virus in water, using a process control. | human hepatitis e virus (hev) causes acute hepatitis in humans, predominantly by contamination of food and water. hev, in particular genotype iii, is currently considered to be an emerging pathogen in industrialized countries. because of the low infectious dose, an efficient and rapid virus concentration method is required to detect low amounts of hev in food and water samples for routine control. because of the absence of a reliable cell culture method for the main enteric viruses most involved ... | 2012 | 22633799 |
| serological prevalence, genetic identification, and characterization of the first strains of avian hepatitis e virus from chickens in korea. | avian hepatitis e virus (avian hev) is associated with hepatitis-splenomegaly (hs) syndrome or big liver and spleen disease in chickens. at least three genotypes of avian hev have been identified from chickens worldwide. a total of 297 serum samples collected from chickens in 35 flocks in korea were tested for avian hev antibody with an enzyme-linked immunosorbent assay. the results showed that approximately 57 % of chicken flocks and 28 % of chickens from korea were positive for antibodies to a ... | 2012 | 22639103 |
| hepatitis e virus-induced severe myositis. | 2012 | 22641093 | |
| hepatitis e virus: neutralizing sites, diagnosis, and protective immunity. | there have been increased attentions on hev and its associated diseases in recent years as a result of an increased number of reports on autochthonous patients from many developed countries. vaccine development and better disease management are expected from protective immunity with increased knowledge on the pathogenesis and virology of hev. this review summarizes the current understanding of the hev virology, the key neutralization sites (epitopes) on the surface of the viral capsid, the host ... | 2012 | 22645002 |
| [forgotten hepatitis: the hepatitis e]. | hepatitis e is caused by the hepatitis e virus (hev), entericaly transmitted. in areas with poor sanitation, hev is responsible for high endemicity and major outbreaks. in countries with high sanitary conditions, hev is a zoonosis responsible for sporadic cases, and is encountered in travellers returning from endemic areas. the clinical manifestations are not distinguishable from that caused by other causes of acute viral hepatitis. fulminant hepatitis are encountered in 1 to 4% of acute hepatit ... | 2012 | 22662626 |
| transmission dynamics of hepatitis e virus in pigs: estimation from field data and effect of vaccination. | hepatitis e is a viral disease that causes serious concerns for public health. hepatitis e virus (hev) genotype 3 is endemic in commercial pig farms worldwide that act as a reservoir. pig-to-human transmission may occur when infectious animals enter the food chain at slaughter, through consumption of contaminated meat, direct exposure or use of by-products. to reduce the fraction of infectious animals at slaughter age and thus the risk for public health, it is important to understand the transmi ... | 2012 | 22664067 |
| serologic study of pig-associated viral zoonoses in laos. | we conducted a serologic survey of four high-priority pig-associated viral zoonoses, japanese encephalitis virus (jev), hepatitis e virus (hev), nipah virus (niv), and swine influenza virus (siv), in laos. we collected blood from pigs at slaughter during may 2008-january 2009 in four northern provinces. japanese encephalitis virus hemagglutination inhibition seroprevalence was 74.7% (95% confidence interval [ci] = 71.5-77.9%), jev igm seroprevalence was 2.3% (95% ci = 1.2-3.2%), and hev seroprev ... | 0 | 22665622 |
| novel approach for detection of hepatitis e virus infection in german blood donors. | the risk of transfusion-transmitted hepatitis e virus (hev) infections by contaminated blood products remains unknown. in the present study, we evaluated and compared different nucleic acid amplification technique (nat) methods for the detection of hev in blood components. minipools of a total of 16,125 individual blood donors were screened for the presence of hev rna using the highly sensitive realstar hev rt-pcr kit, revealing a minimum detection limit of 4.66 iu/ml. thirteen donors were hev r ... | 2012 | 22675127 |
| hepatitis e virus infection causing isolated fetal ascites: a case report. | maternal hepatitis infection, excepting hepatitis e, causing isolated fetal ascites with variable outcome has been reported previously. we present a case of maternal hepatitis e virus (hev) infection causing isolated fetal ascites which resolved spontaneously during pregnancy and resulted in a term live-born baby with anti-hev seropositivity. a 39-year-old primigravida woman was diagnosed with acute hev infection at 15 weeks of gestation. ultrasound at 19 weeks showed significant fetal ascites w ... | 2012 | 22677891 |
| seroprevalence of hepatitis e virus among blood donors in a district of southern italy. | 2012 | 22682344 | |
| detection of igm and igg against hepatitis e virus in serum and meat juice samples from pigs at slaughter in bavaria, germany. | hepatitis e virus (hev) is an emerging foodborne pathogen with domestic and wild pigs (and likely other species such as deer or rabbits) recognized as reservoir. pathogenesis in pigs usually leads to an asymptomatic course of disease. since there is no enzyme-linked immunosorbent assay (elisa) kit for the detection of anti-hev antibodies in pigs commercially available, the objective of this study was to assess the seroprevalence in fattening pigs at slaughter and at herd level using a newly deve ... | 2012 | 22690762 |
| bats worldwide carry hepatitis e virus-related viruses that form a putative novel genus within the family hepeviridae. | hepatitis e virus (hev) is one of the most common causes of acute hepatitis in tropical and temperate climates. tropical genotypes 1 and 2 are associated with food-borne and waterborne transmission. zoonotic reservoirs (mainly pigs, wild boar, and deer) are considered for genotypes 3 and 4, which exist in temperate climates. in view of the association of several zoonotic viruses with bats, we analyzed 3,869 bat specimens from 85 different species and from five continents for hepevirus rna. hevs ... | 2012 | 22696648 |
| hepatitis e vaccine development: a 14 year odyssey. | the first prophylactic vaccine, hecolin®, against hepatitis e virus (hev) infection and the hev associated disease was approved by china's state food and drug administration (sfda) in december 2011. key milestones during the 14-year hev vaccine development are summarized in this commentary. after years of innovative research the recombinant virus-like particle (vlp) based antigen with virion-like epitopes was successfully produced in e. coli production platform on a commercial scale. safety and ... | 2012 | 22699438 |
| diagnosis of hev infection by serological and real-time pcr assays: a study on acute non-a-c hepatitis collected from 2004 to 2010 in italy. | the impact of hepatitis e in developed countries, like italy, still requires a clear definition. in the present study, we evaluated hev infection in patients with acute non-a-c hepatitis by an approach comparing data from real-time pcr and serological assays. | 2012 | 22704073 |
| molecular epidemiology and genetic history of european-type genotype 3 hepatitis e virus indigenized in the central region of japan. | in mie prefecture in japan, 12 cases of sporadic hepatitis e occurred from 2004 to 2011. mie prefecture is located in the central region of japan, far from the most prevalent regions of hepatitis e virus (hev) infection in japan, the north and northeastern part. these 12 cases did not have any common risk factors of hev infection. we analyzed the molecular epidemiology of the cases in mie prefecture. we obtained the nucleotide sequences of the hev strains and analyzed them with the sequences of ... | 2012 | 22706162 |
| [detection and description of avian hepatitis e virus isolated in china--a review]. | avian hepatitis e virus (hev), a member of hepeviridae family, is genetically and antigenically related with human and swine hev in the family. since its discovery, avian hev infection has been investigated in many countries from serology and molecular epidemiology studies. at present, five complete or near complete genomes of avian hev isolates were reported in genbank and were divided into three genotypes. the complete genome of avian hev contains 3 orfs of which orf2 gene encodes capsid prote ... | 2012 | 22712397 |
| high hepatitis e virus seroprevalence in forestry workers and in wild boars in france. | hepatitis e virus (hev) is a fecally and orally transmitted human pathogen of worldwide distribution. in industrial countries, hev is observed in an increasing number of autochthonous cases and is considered to be an emerging pathogen. a growing body of evidence suggests that hev is a zoonotic disease, and pig handlers and pig veterinarians have been reported to be high-risk groups for hev infection. the aims of the present study were to establish the prevalence of anti-hev in wild boars in fran ... | 2012 | 22718947 |
| seroprevalence of anti-hepatitis e virus (hev) in a korean population: comparison of two commercial anti-hev assays. | hepatitis e virus (hev) has emerged as an important cause of epidemic and sporadic acute viral hepatitis worldwide. this study investigated the seroprevalence of anti-hev in a korean population and compared the performance of two commercially available anti-hev assays. | 2012 | 22726615 |
| viral hepatitis in india. | hepatitis in india is caused mainly by hepatitis a virus (hav), hepatitis b virus (hbv), hepatitis c virus (hcv), and hepatitis e virus (hev). hav infection occurs frequently in children, though in parts of india there is an evolving epidemiology. hev is the most common cause of acute sporadic hepatitis in india and has been associated with several large-scale epidemics in the past. india belongs to the intermediate endemicity zone for hbv carriers. hbv is the major cause of chronic liver diseas ... | 2012 | 22726997 |
| presence of hepatitis e rna in mussels used as bio-monitors of viral marine pollution. | mussels (mytilus galloprovincialis), collected from a harvesting area approved by european community regulation, were transplanted to four polluted sites located in the northwestern mediterranean area (tuscany). they were used as bio-monitors to test the quality of the marine water pollution. at different times after the transplantation, mussels were withdrawn and tested for presence of phages and enteric viruses by molecular tests. 52.4% of the transplanted mussel samples were positive for at l ... | 2012 | 22728271 |
| seroepidemiology of hepatitis e virus in western iran. | 2017 | 22729203 | |
| [genetic analysis of echovirus 30 isolated from acute meningitis/encephalitis syndrome cases in linyi city of shandong province, china]. | to investigate the pathogen spectrum constitution of hev related to acute meningitis/encephalitis syndrome (ames) and to analyze the genetic characterization of the echovirus 30 (echo30) isolates. | 2011 | 22734221 |
| [hepatitis c viruses infection situation in the human population of six provinces in china]. | to investigate the seroprevalence of hepatitis c viruse infection in the human population in six regions of beijing, heilongjiang, shandong, ningxia, gansu and sichuan in china. | 2011 | 22734232 |
| prevalence of hepatitis e virus infection in pediatric solid organ transplant recipients--a single-center experience. | hev infection appears to be an emerging disease in industrialized countries. the aim of this study was to evaluate the prevalence of hev infection in pediatric solid organ transplant recipients. one hundred and twenty-four pediatric recipients of liver (n = 41) or kidney (n = 83) transplants aged between one and 18 yr were screened for anti-hev igg antibodies. patients were tested for fecal hev rna excretion if they showed anti-hev seropositivity. as a control group, 108 immunocompetent pediatri ... | 2012 | 22738211 |
| immature and mature human astrovirus: structure, conformational changes, and similarities to hepatitis e virus. | human astroviruses (hastvs) are a major cause of gastroenteritis. hastv assembles from the structural protein vp90 and undergoes a cascade of proteolytic cleavages. cleavage to vp70 is required for release of immature particles from cells, and subsequent cleavage by trypsin confers infectivity. we used electron cryomicroscopy and icosahedral image analysis to determine the first experimentally derived, three-dimensional structures of an immature vp70 virion and a fully proteolyzed, infectious vi ... | 2012 | 22743104 |
| the burden of liver disease in human immunodeficiency virus-infected patients. | introduction of effective combined antiretroviral therapy has made human immunodeficiency virus (hiv) infection a chronic illness. substantial reductions in the number of acquired immunodeficiency syndrome- (aids-) related deaths have been accompanied by an increase in liver-related morbidity and mortality. liver diseases rank in the first three most-common causes of death in hiv-infected persons. mortality is mainly due to cirrhosis and hepatocellular carcinoma induced by hepatitis c virus and ... | 2012 | 22760649 |
| hepatitis e virus quasispecies and the outcome of acute hepatitis e in solid-organ transplant patients. | hepatitis e virus (hev) infections are responsible for chronic hepatitis in immunocompromised patients, and this can evolve to cirrhosis. like all rna viruses, hev exists as a mixture of heterogeneous viruses defining quasispecies. the relationship between the genetic heterogeneity described as a quasispecies, cytokine secretion, and the outcome of acute hepatitis in immunocompromised patients remains to be elucidated. we cloned and sequenced the region encoding the m and p capsid domains of hev ... | 2012 | 22761386 |
| molecular detection and characterization of hepatitis e virus in naturally infected pigs from brazilian herds. | the aim of this study was to investigate the presence of swine hepatitis e virus (hev) from pigs of different production categories and from different pig farms in south brazil. a total of 170 porcine faecal samples from breeder sows, boars, suckling piglets, weaned and growing pigs were collected from 14 pig farms. the faecal samples were screened by nested rt-pcr using primers targeting the orf2 region of hev genome. the samples that were positive from this screening were used in a nested rt-p ... | 2012 | 22769738 |
| complete genome sequence of the genotype 4 hepatitis e virus strain prevalent in swine in jiangsu province, china, reveals a close relationship with that from the human population in this area. | hepatitis e virus (hev) is a zoonotic pathogen of which several species of animal were reported as reservoirs. swine stands out as the major reservoir for hev infection in humans, as suggested by the close genetic relationship of swine and human viruses. in a previous study, we sequenced the complete genome of a human genotype 4 hev strain (hm439284) that is prevalent in jiangsu province, china. here we report the complete genome of one genotype 4 hev strain which is prevalent in swine herds in ... | 2012 | 22787267 |
| evolution of the hepatitis e virus polyproline region: order from disorder. | the hepatitis e virus (hev) polyproline region (ppr) is an intrinsically unstructured region (idr). this relaxed structure allows idrs, which are implicated in the regulation of transcription and translation, to bind multiple ligands. originally the nucleotide variability seen in the hev ppr was assumed to be due to high rates of insertion and deletion. this study shows that the mutation rate is about the same in the ppr as in the rest of the nonstructural polyprotein. the difference between the ... | 2012 | 22811526 |
| seroprevalence of hepatitis e virus in humans and geographically matched food animals in egypt. | the aim of current study was to investigate the epidemiology of hepatitis e virus (hev) in humans and geographically matched food animals as a novel zoonotic assessment in egypt. blood samples were collected from patients who had a history of jaundice and attended to fever and general hospitals. animal blood samples were collected from cows, buffaloes, sheep and goats convenient to hev seropositive humans. enzyme immuno assay (eia) protocol was used to determine igg anti-hev. sex and pregnancy w ... | 2013 | 22812407 |
| chronic hepatitis e in heart transplant recipients. | chronic courses of hepatitis e virus (hev) infections have been described in immunosuppressed patients. we aimed to study the role of hev infections in heart transplant recipients (htr). 274 htr were prospectively screened for hev infection using an anti-hev-igg elisa and hev-pcr. in addition, 137 patients undergoing cardiac surgery (non-htr) and 537 healthy subjects were studied cross-sectionally. the anti-hev-igg seroprevalence was 11% in htr, 7% in non-htr and 2% in healthy controls (htr vs. ... | 2012 | 22823202 |
| recombination and natural selection in hepatitis e virus genotypes. | to gain new insights into the evolutionary processes that created the genetic diversity of the hepatitis e virus (hev), the recombination detection program (rdp) and simplot program were employed to detect recombination events in the genome, then the fixed-effects likelihood (fel) method was used to detect natural selection effects on viral proteins. recombination analysis provided strong evidence for both intergenotype and intragenotype recombination events in the sequences analyzed. recombinat ... | 2012 | 22825818 |
| seroepidemiology of hepatitis a and e virus infections in tehran, iran: a population based study. | hepatitis a virus (hav) and hepatitis e virus (hev) are enteric hepatotropic viruses and their prevalence is related to the sanitary conditions of the region under investigation. there are only a few studies on the seroepidemiology of these two viruses in the general iranian population. the purpose of this investigation was to measure the prevalence of hepatitis a and e infections in the general population. between 2006 and 2007, a cross sectional study was performed in tehran, iran. blood speci ... | 2012 | 22835757 |
| circulation of almost genetically identical hepatitis e virus of genotype 4 in france. | 2012 | 22835777 | |
| rescue of a genotype 4 human hepatitis e virus from cloned cdna and characterization of intergenotypic chimeric viruses in cultured human liver cells and in pigs. | hepatitis e virus (hev) is an important but extremely understudied human pathogen. genotypes 1 and 2 are restricted to humans, whereas genotypes 3 and 4 are zoonotic, infecting both humans and pigs. this report describes, for the first time, the successful rescue of infectious hev in vitro and in vivo from cloned cdna of a genotype 4 human hev (strain tw6196e). the complete genomic sequence of the tw6196e virus was determined and a full-length cdna clone (phev-4tw) was assembled. capped rna tran ... | 2012 | 22837416 |
| evolution of the hepatitis e virus hypervariable region. | the presence of a hypervariable (hvr) region within the genome of hepatitis e virus (hev) remains unexplained. previous studies have described the hvr as a proline-rich spacer between flanking functional domains of the orf1 polyprotein. others have proposed that the region has no function, that it reflects a hypermutable region of the virus genome, that it is derived from the insertion and evolution of host sequences or that it is subject to positive selection. this study attempts to differentia ... | 2012 | 22837418 |
| feeding of the probiotic bacterium enterococcus faecium ncimb 10415 differentially affects shedding of enteric viruses in pigs. | effects of probiotic bacteria on viral infections have been described previously. here, two groups of sows and their piglets were fed with or without feed supplementation of the probiotic bacterium enterococcus faecium ncimb 10415. shedding of enteric viruses naturally occurring in these pigs was analyzed by quantitative real-time rt-pcr. no differences between the groups were recorded for hepatitis e virus, encephalomyocarditis virus and norovirus. in contrast, astrovirus was exclusively detect ... | 2012 | 22838386 |
| hepatitis e virus in pork food chain, united kingdom, 2009-2010. | we investigated contamination by hepatitis e virus (hev) in the pork production chain in the united kingdom. we detected hev in pig liver samples in a slaughterhouse, in surface samples from a processing plant, and in pork sausages and surface samples at point of sale. our findings provide evidence for possible foodborne transmission of hev during pork production. | 2012 | 22840183 |
| autochthonous infections with hepatitis e virus genotype 4, france. | during january-march 2011, diagnoses of hepatitis e virus (hev) infection increased in marseille university hospitals in southeastern france. hev genotype 4, which is described almost exclusively in asia, was recovered from 2 persons who ate uncooked pork liver sausage. genetic sequences were 96.7% identical to those recently described in swine in europe. | 2012 | 22840196 |
| hepatitis e virus genotype 3 in wild rats, united states. | the role of rodents in the epidemiology of zoonotic hepatitis e virus (hev) infection has been a subject of considerable debate. seroprevalence studies suggest widespread hev infection in commensal rattus spp. rats, but experimental transmission has been largely unsuccessful and recovery of zoonotic genotype 3 hev rna from wild rattus spp. rats has never been confirmed. we surveyed r. rattus and r. norvegicus rats from across the united states and several international populations by using a hem ... | 2012 | 22840202 |
| putative novel genotype of avian hepatitis e virus, hungary, 2010. | to explore the genetic diversity of avian hepatitis e virus strains, we characterized the near-complete genome of a strain detected in 2010 in hungary, uncovering moderate genome sequence similarity with reference strains. public health implications related to consumption of eggs or meat contaminated by avian hepatitis e virus, or to poultry handling, require thorough investigation. | 2012 | 22840214 |
| hepatitis e virus strains in rabbits and evidence of a closely related strain in humans, france. | hepatitis e virus (hev) strains from rabbits indicate that these mammals may be a reservoir for hevs that cause infection in humans. to determine hev prevalence in rabbits and the strains' genetic characteristics, we tested bile, liver, and additional samples from farmed and wild rabbits in france. we detected hev rna in 7% (14/200) of bile samples from farmed rabbits (in 2009) and in 23% (47/205) of liver samples from wild rabbits (in 2007-2010). full-length genomic sequences indicated that all ... | 2012 | 22840216 |
| novel hepatitis e virus in ferrets, the netherlands. | 2012 | 22840220 | |
| hepatitis e virus in pork production chain in czech republic, italy, and spain, 2010. | we evaluated the prevalence of hepatitis e virus (hev) in the pork production chain in czech republic, italy, and spain during 2010. a total of 337 fecal, liver, and meat samples from animals at slaughterhouses were tested for hev by real-time quantitative pcr. overall, hev was higher in italy (53%) and spain (39%) than in czech republic (7.5%). hev was detected most frequently in feces in italy (41%) and spain (39%) and in liver (5%) and meat (2.5%) in czech republic. of 313 sausages sampled at ... | 0 | 22840221 |
| hepatitis e virus infection in ghanaian blood donors - the importance of immunoassay selection and confirmation. | hepatitis e virus (hev) infection is emerging as a potential new threat to blood safety after several cases of transfusion-transmission were reported from non-epidemic countries. on the basis of seroprevalence data, hev is endemic in ghana where poor sanitary conditions and regular flooding are prevalent. however, no data are available for hev prevalence in blood donors. | 2013 | 22845878 |
| detection and molecular characterization of hepatitis e virus in clinical, environmental and putative animal sources. | putative animal reservoirs and environmental samples were studied to investigate potential routes of transmission for indigenous hepatitis e virus (hev) infection in hokkaido, japan. a total of 468 liver samples and 954 environmental samples were collected from 2003 to 2011 for this study. four swine livers (1 %) were positive for hev rna; two strains belonged to genotype 3 and the other two strains were genotype 4. genotype 3 hev was detected in a sewage sample and a seawater sample. hev strain ... | 2012 | 22847755 |
| detection and phylogenetic analysis of hepatitis e viruses from mongooses in okinawa, japan. | hepatitis e virus (hev) infection has previously been reported in wild mongooses on okinawa island; to date however, only one hev rna sequence has been identified in a mongoose. hence, this study was performed to detect hev rna in 209 wild mongooses on okinawa island. six (2.9%) samples tested positive for hev rna. phylogenetic analysis revealed that 6 hev rnas belonged to genotype 3 and were classified into groups a and b. in group b, mongoose-derived hev sequences were very similar to mongoose ... | 2012 | 22850461 |
| simple and rapid detection of swine hepatitis e virus by reverse transcription loop-mediated isothermal amplification. | hepatitis e virus (hev) is an enteric pathogen of humans and animals, and pigs have been considered an important reservoir of this virus. recent evidence has indicated the cross-species transmission of hepatitis e virus (hev) from pigs to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. in this study, we have developed a one-step rt-lamp assay for rapid detection of swine hev. specific primer sets targeting the orf3 gene were designed. the sensitiv ... | 2012 | 22855125 |
| seroprevalence of anti-hepatitis e virus and anti-salmonella antibodies in pigs at slaughter in switzerland. | hepatitis e virus (hev) and salmonella bacteria are zoonotic pathogens that can be acquired by foodborne transmission because food animals, for example pigs, are recognized as a reservoir. the objectives of this study were to determine the seroprevalence of anti-hev immunoglobulin g (igg) and anti-salmonella antibodies from healthy pigs at slaughter in switzerland, a country with a good health status of pig herds (e.g., eradication of enzootic pneumonia) compared with those of many countries in ... | 2012 | 22856574 |
| assessment of f-rna coliphage as a potential indicator of enteric virus contamination of hog carcasses. | hepatitis e virus (hev) is common in pigs, and some swine hev strains are closely related to human strains. the zoonotic transmission of hev is now well established. hev can be detected by molecular techniques, but the significance of the presence of viral nucleic acid is questionable when foods are subjected to virus inactivation treatments. f-rna coliphages are attractive candidates as indicators for enteric viruses because they are similar in size and survival characteristics and can be rapid ... | 2012 | 22856576 |
| chronic hepatitis e after solid organ transplantation. | large outbreaks of acute hepatitis e, caused by hepatitis e virus (hev) genotypes 1 and 2, are known from developing countries with suboptimal sanitation infrastructure. an increasing incidence of hev infections is being reported in industrialised countries, caused mainly by hev genotypes 3 and 4, which are often found among pigs. recent evidence suggests that in immunocompromised patients about 50% of the cases of acute hepatitis e evolve to chronic hepatitis with rapid progression to cirrhosis ... | 2012 | 22859417 |
| meld score: utility and comparison with king's college criteria in non-acetaminophen acute liver failure. | to compare model for end-stage liver disease score (meld score, ms) and king's college hospital (kch) criteria for finding correlation of mortality in non-acetaminophen induced acute liver failure (nai-alf). | 2012 | 22868013 |
| minor groove binder modification of widely used taqman probe for hepatitis e virus reduces risk of false negative real-time pcr results. | hepatitis e virus (hev) is a major cause of acute viral hepatitis in many parts of the developing world. it is responsible for both sporadic infections and large scale epidemics and may be associated with significant mortality during pregnancy. over the past two decades many serological and nucleic acid based diagnostic tests for hev have been developed, including several reverse transcription real-time polymerase chain reaction assays (rt-qpcr). one of the most widely used of these rt-qpcrs is ... | 2012 | 22871672 |
| seroprevalence of hepatitis e virus varies considerably among chinese provinces. | hepatitis e is a common infection in china, but few studies have been carried out to compare regional and ethnic factors in its prevalence. | 2012 | 22879828 |
| assessment of the cross-protective capability of recombinant capsid proteins derived from pig, rat, and avian hepatitis e viruses (hev) against challenge with a genotype 3 hev in pigs. | hepatitis e virus (hev), the causative agent of hepatitis e, is primarily transmitted via the fecal-oral route through contaminated water supplies, although many sporadic cases of hepatitis e are transmitted zoonotically via direct contact with infected animals or consumption of contaminated animal meats. genotypes 3 and 4 hev are zoonotic and infect humans and other animal species, whereas genotypes 1 and 2 hev are restricted to humans. there exists a single serotype of hev, although the cross- ... | 2012 | 22906724 |
| serological evidence for a hepatitis e virus-related agent in goats in the united states. | hepatitis e virus (hev) causes an important public health disease in many developing countries and is also endemic in some industrialized countries. in addition to humans, strains of hev have been genetically identified from pig, chicken, rat, mongoose, deer, rabbit and fish. while the genotypes 1 and 2 hev are restricted to humans, the genotypes 3 and 4 hev are zoonotic and infect humans and other animal species. as a part of our ongoing efforts to search for potential animal reservoirs for hev ... | 2013 | 22909079 |
| hepatitis in the elderly: still a scourge. | liver disease presents with classic symptoms: fatigue, anorexia that progresses to nausea and vomiting, muscle and joint pain, and jaundice. its most common cause is viral infection (hepatitis) with one of the hepatotropic hepatitis viruses. although all types of hepatitis cause liver disease, their modes of transmission differ, and treatment may or may not be an option. in all types of hepatitis, people older than 65 years of age tend to develop more severe disease than those who are younger. h ... | 2012 | 22910128 |
| ribavirin therapy for hepatitis e virus-induced acute on chronic liver failure: a preliminary report. | hepatitis e virus (hev) is a common cause of acute on chronic liver failure (aclf) in hev hyperendemic regions with high mortality. treatment for hev-induced aclf is currently not available. recently, efficacy of ribavirin in genotype 3 chronic hepatitis e patients has been reported; however, whether ribavirin is effective in genotype 1 hev infection is not yet known. the present study includes four patients with hev-induced aclf treated with ribavirin in a genotype 1 hev hyperendemic region. di ... | 2012 | 22910532 |
| zoonotic hepatitis e virus strains in german blood donors. | 2013 | 22913247 | |
| [the coastal areas of yantai human and swine hepatitis e virus genotyping analysis]. | survey of the coastal city of yantai, from human and swine hepatitis e virus (hev) genotype correlation. | 2012 | 22919748 |
| [antibody detection of hepatitis e virus in human population of different national in china]. | to investigate the seroprevalence of hev infection in different national human population in han, hui and zang in china. | 2012 | 22919753 |
| first detection of hepatitis e virus in austrian pigs by rt-qpcr. | long known to cause disease outbreaks in man in countries with poor sanitary conditions, an increasing number of autochthonous hev genotype 3 infections have been reported in industrialised countries. genotype 3 poses an important potential zoonotic threat, with infected pigs functioning as the main reservoir. this study reports the first detected emergence of hev in austrian pigs. five austrian strains were partially sequenced and phylogenetic analysis demonstrates that they cluster within geno ... | 2012 | 22919921 |