Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| primary cervical carcinomas show 2 common regions of deletion at 3p, 1 within the fhit gene: evaluation of allelic imbalance at fhit, rb1 and tp53 in relation to survival. | chromosome arm 3p is re-arranged in many tumor types, including cervical carcinomas. putative tumor-suppressor genes on 3p have been proposed, including the fhit gene, which maps to chromosome band 3p14.2. we have analyzed 79 primary cervical carcinomas for allelic imbalance (ai) at 17 chromosome 3 loci, including 3 within the fhit gene. expression of the fhit gene was evaluated after immunohistochemistry with an antibody against the pfhit protein. previously determined human papillomavirus stat ... | 2000 | 11004671 |
| differential distribution of sequence variations in hpv-16 e6. | the e6 regions of the oncogenic human papillomaviruses (hpvs) are important in carcinogenesis and immune recognition. we examined the e6 dna sequence from hpv-16-associated cervical cancers to determine the frequency and degree of variation from the consensus sequence in selected populations. | 2000 | 11006023 |
| significance of atypical squamous cells of undetermined significance on thinprep papanicolaou smears. | the aim of this study was to assess the prevalence and risk factors predictive of dysplasia among women seen in a gynecologic oncology service with the cytologic diagnosis of atypical squamous cells of undetermined significance (ascus) on papanicolaou smears obtained by the thinprep method. | 2000 | 11006029 |
| correlates of the spread of human papillomavirus infection. | knowledge of the correlates of human papillomavirus (hpv) seropositivity is of interest for planning of preventive measures and for evaluation of possible confounding in epidemiological studies. the epidemiological determinants for seropositivity for oncogenic and benign hpv types were assessed using a serosurvey of 275 healthy swedish women, stratified by age and lifetime number of sexual partners. seroprevalences were compared with 17 behavioral variables obtained by interview and 3 laboratory ... | 2000 | 11008914 |
| the prevalence of genital human papillomavirus infections in abused and nonabused preadolescent girls. | to compare the prevalence of genital human papillomavirus (hpv) infections in sexually abused and nonabused preadolescent girls and assess the feasibility of conducting a longitudinal study of the natural history of hpv infection in this population. | 2000 | 11015503 |
| use of hormone replacement therapy (hrt) and detection of human papillomavirus (hpv) dna in postmenopausal women. | purpose: this study investigated the association between detection of hpv dna among current and past hrt users compared to never hrt users, duration of hrt use, and type of hrt (estrogen, estrogen/progestin).methods: postmenopausal women (n = 390) were recruited from a university hospital and completed a questionnaire regarding 1) hrt use, duration, and type, 2) reproductive and sexual history, 3) smoking and alcohol use, and 4) hpv-related diseases. cervical specimens were obtained for pap smea ... | 2000 | 11018386 |
| detection of human papillomavirus in epithelial lesions of the conjunctiva. | many factors like exposure to uv radiation, climatic conditions, genetic predisposition, immunological state and, more recently, the presence of hpv have been implicated in the genesis of some lesions of the conjunctiva, especially the carcinoma. | 2000 | 11018845 |
| treatment of external genital warts in men with imiquimod 2% in cream. a placebo-controlled, double-blind study. | the purpose of this double-blind, placebo-controlled study was to evaluate the safety, clinical efficacy and tolerability of imiquimod (2%) in cream to cure external genital warts in males. | 2000 | 11023759 |
| the e1e4 protein of human papillomavirus type 16 associates with a putative rna helicase through sequences in its c terminus. | human papillomavirus type 16 (hpv16) infects cervical epithelium and is associated with the majority of cervical cancers. the e1e4 protein of hpv16 but not those of hpv1 or hpv6 was found to associate with a novel member of the dead box protein family of rna helicases through sequences in its c terminus. this protein, termed e4-dbp (e4-dead box protein), has a molecular weight of 66,000 (66k) and can shuttle between the nucleus and the cytoplasm. it binds to rna in vitro, including the major hpv ... | 2000 | 11024137 |
| a longitudinal study of hpv detection and cervical pathology in hiv infected women. | to monitor the presence and persistence of high risk (hr) human papillomavirus (hpv) in cervical brushings from hiv infected women. | 2000 | 11026879 |
| histological diagnosis of cutaneous "warty" carcinoma on a pre-existing hpv lesion. | a middle-aged man with an 8-year history of a fungating tumour mass on his thigh was histologically diagnosed as having an invasive "warty" carcinoma at the location of a pre-existing human papillomavirus (hpv) lesion. the tumour surface had a verruciform appearance with papillae containing fibrovascular cores. many of the malignant cells displayed changes consistent with koilocytotic atypia. we noticed a greater degree of nuclear atypia in comparison with a verrucous carcinoma. focally, some ne ... | 2000 | 11028866 |
| bovine dental papilla-derived cells immortalized with hpv 18 e6/e7. | in vitro investigations of cell-specific metabolism and cell interactions as well as biocompatibility studies are often hampered by the limited lifespan of primary cells originating from target tissues like the oral mucosa, gingiva or pulp. pulp cells, as do other primary cells, undergo senescence after several passages in vitro. however, senescence can be overcome by transfection of primary cells with oncogenes like the hpv 18 (human papillomavirus 18) e6/e7 oncogene, resulting in immortalized ... | 2000 | 11037760 |
| [verification of identification of infectious agents by culture in routine gynecologic cytological screening]. | the original bethesda classification system for reporting cervical/vaginal cytologic diagnoses has claimed besides oncologic evaluation also a statement on the presence of infectious agents. their diagnosis should be followed by appropriate treatment. based upon the comparison of careful bacterioscopic study in a series of 175 routine cervical smears with the results of microbiological, virological and mycological examinations the following pathogens might be-according to the authors' opinion-di ... | 1999 | 11038666 |
| recent striking changes in histological differentiation and rate of human papillomavirus infection in squamous cell carcinoma of the lung in okinawa, a subtropical island in southern japan. | the incidence of lung cancer in okinawa has been the highest in japan since 1975, and squamous cell carcinoma (scc), especially the well differentiated form, is the most prevalent form in okinawa, although well differentiated scc is relatively rare in mainland japan. furthermore, a high proportion of scc of the lung in okinawa was positive for human papillomavirus (hpv). in this study, we report recent striking changes in histological features and in the incidence of hpv infection. | 2000 | 11041057 |
| hybrid capture based human papillomavirus typing in cervical screening compared to cytology and histology. | cervical cancer is frequently associated with infection from various types of human papillomavirus (hpv) with high a oncogenic potential (high-risk types). commercial systems for hpv typing are available, but the question as to when hpv typing should be performed has not yet been solved. | 2000 | 11042905 |
| p53 codon 72 polymorphism in vulval cancer and vulval intraepithelial neoplasia. | p53 codon 72 polymorphism was analysed in uk women with human papillomavirus (hpv)-associated vulval intraepithelial neoplasia and vulval squamous cell carcinoma. arginine homozygotes were significantly less common in either group compared with controls. we conclude that the arginine polymorphism may confer protection against the development of hpv-associated vulval neoplasia. | 2000 | 11044351 |
| [vulvar lesions caused by hpv]. | human papillomavirus subclinical lesions are well known on the cervix and are identified by colposcopy after acetic acid staining. the transfer of this technique from the cervix to the vulva has led to the identification of areas of white epithelial changes which have been defined by similarity as vulvar subclinical hpv (vshpv) lesions. a critical revision of the different clinical vshpv lesions classifications, the vulvar diagnostic role of acetic acid staining, the natural history of hpv infec ... | 2000 | 11048476 |
| prevalence of human papillomavirus infection and its correlation with cervical lesions in commercial-sex workers in japan. | to investigate the prevalence of the human papillomavirus (hpv) infection and its correlation with cervical lesions in commercial-sex workers (csws) who attended a sexually transmitted disease (std) clinic in an entertainment area in tokyo. | 2000 | 11049234 |
| risk factors for cervical intraepithelial neoplasia in southwestern american indian women. | the authors assessed risk factors for cervical intraepithelial neoplasia (cin) among southwestern american indian women using case-control methods. cases were new mexico american indian women with biopsy-proven grade i (n = 190), grade ii (n = 70), or grade iii (n = 42) cervical lesions diagnosed between november 1994 and october 1997. controls were american indian women from the same indian health service clinics with normal cervical epithelium (n = 326). all subjects underwent interviews and l ... | 2000 | 11052549 |
| na+/h+ exchanger-dependent intracellular alkalinization is an early event in malignant transformation and plays an essential role in the development of subsequent transformation-associated phenotypes. | in this study we investigate the mechanism of intracellular ph change and its role in malignant transformation using the e7 oncogene of human papillomavirus type 16 (hpv16). infecting nih3t3 cells with recombinant retroviruses expressing the hpv16 e7 or a transformation deficient mutant we show that alkalinization is transformation specific. in nih3t3 cells in which transformation can be turned on and followed by induction of the hpv16 e7 oncogene expression, we demonstrate that cytoplasmic alka ... | 2000 | 11053239 |
| oral focal epithelial hyperplasia in a howler monkey (alouatta fusca). | oral focal epithelial hyperplasia is a rare and seldom reported disease in animals and humans induced by a papillomavirus. the present report is the first description of this disease in a neotropical primate, a howler monkey (alouatta fusca). the diagnosis was based on gross and microscopic findings. the generic papillomavirus antigen was identified by immunohistochemistry and was found not to be related to any human papillomavirus dna tested by in situ hybridization. this virus is probably a sp ... | 2000 | 11055880 |
| subcytotoxic h2o2 stress triggers a release of transforming growth factor-beta 1, which induces biomarkers of cellular senescence of human diploid fibroblasts. | stress-induced premature senescence (sips) is induced 3 days after exposure of human diploid fibroblasts to subcytotoxic oxidative stress with h(2)o(2), with appearance of several biomarkers of replicative senescence. in this work, we show that transforming growth factor-beta1 (tgf-beta1) regulates the induction of several of these biomarkers in sips: cellular morphology, senescence-associated beta-galactosidase activity, increase in the steady-state level of fibronectin, apolipoprotein j, osteo ... | 2001 | 11060295 |
| immortalization of a human prostate stromal cell line using a recombinant retroviral approach. | we established an immortalized human prostate stromal cell line with retained markers of cell differentiation and alpha1-adrenergic receptor expression. | 2000 | 11061945 |
| contribution of human papillomavirus testing by hybrid capture in the triage of women with repeated abnormal pap smears before colposcopy referral. | the purpose of this work was to evaluate the ability of testing for high-risk human papillomavirus (hpv) types using the hybrid capture technique to predict the presence of cervical intraepithelial neoplasia (cin) ii,iii in patients with repeated atypical squamous cells of undetermined significance (ascus) and low-grade squamous intraepithelial lesion (lgsil) on pap smears. | 2000 | 11063640 |
| persistence of human papillomavirus infection after therapeutic conization for cin 3: is it an alarm for disease recurrence? | the aims of this study were (1) to examine whether hpv dna is persistently detected in the cervix after therapeutic conization for cin 3 and (2) to explore whether a patient with persistence of hpv infection is at risk of developing recurrent disease. | 2000 | 11063660 |
| punctate keratoderma-like lesions on the palms and soles in a patient with chloracne: a new clinical manifestation of dioxin intoxication? | we report what we believe to be a novel skin manifestation of dioxin intoxication. a 30-year-old woman with 2,3,7, 8-tetrachlorodibenzo-p-dioxin levels of 144,000 pg g-1 blood fat presented with severe chloracne that affected the entire integument. she also exhibited acral granuloma annulare-like lesions and distal onycholysis and, at a later time point, showed signs of hypertrichosis, as well as brownish-grey hyperpigmentation of the face. in addition, she developed punctate keratoderma-like le ... | 2000 | 11069523 |
| telomerase in (pre)neoplastic cervical disease. | this study was performed to determine upregulation of the human telomerase rna component (htr) and mrna of the catalytic subunit of telomerase (htert) in (pre)malignant cervical lesions, to analyze possible intralesional heterogeneity of htr expression, and to relate htr and htert mrna levels to telomerase activity levels and human papillomavirus (hpv) typing. htr expression was determined by in situ hybridization (ish) on paraffin-embedded sections, obtained from patients with cervical intraepi ... | 2000 | 11070123 |
| preliminary report on a method of human papillomavirus dna extraction from verruca plantaris for polymerase chain reaction studies. | this preliminary report on the chelex-based extraction method of hpv dna from verruca plantaris is the first step in a series of studies on verruca plantaris undertaken, jointly, by the departments of podiatric medicine and microbiology at des moines university-osteopathic medical center, des moines, iowa. current projects include the development of new primers for pcr studies of hpv, exploration of different viral dna extraction methods, and surveys concerning treatment, success of therapies, a ... | 2000 | 11070803 |
| presence of human papillomavirus and epstein-barr virus in the cervix of women infected with the human immunodeficiency virus. | the presence of human papillomavirus (hpv) and epstein-barr virus (ebv) was sought in cervical scrapings from 110 human immunodeficiency virus (hiv)-infected women to evaluate the role of these viruses as risk factors for squamous intraepithelial lesions of the cervix. by using pcr, presence of hpv-dna and ebv-dna was found in 60.9% (67/110) and in 10% (11/110) of clinical samples, respectively. identification of oncogenic group of hpv by hybrid capture (hc ii, murex-digene) indicated the presen ... | 2000 | 11074467 |
| pagetoid dyskeratosis of the cervix: an incidental histologic finding in uterine prolapse. | pagetoid dyskeratosis, is considered a reactive process in which a small part of the normal population of keratinocytes is induced to proliferate. the lesion is characterized by pale cells resembling those of paget's disease within the epidermis. these cells have been seen as an incidental finding in a variety of benign papules most commonly located in intertriginous areas. among the inductors of the lesion, friction is suspected. to the best of our knowledge, these pale cells have not been repo ... | 2000 | 11075853 |
| prediction of high-grade cervical intraepithelial neoplasia in cytologically normal women by human papillomavirus testing. | human papillomavirus (hpv) testing has been suggested for primary screening of cervical cancer. prediction of future high-grade cervical lesions is crucial for effectiveness and cost. we performed a case control study in a retrospective cohort of women with at least two cervical smears, all but the last one being negative, from the organized cervical screening programme in florence, italy. we searched for high-risk hpv in all previous, archival, smears from cases (new histologically confirmed ce ... | 2000 | 11076654 |
| the clinical importance of atypical glandular cells of undetermined significance on the cytologic smear. | the objective of the study was to assess the prevalence of underlying cervical and endometrial lesions among patients with atypical glandular cells of undetermined significance (agcus) on the cytologic smear. eighty-six patients with agcus, without evidence of squamous intraepithelial lesions, underwent coloposcopy and endocervical curettage (ecc) within 6 months of the initial finding. endometrial samplings were performed in 25 patients. coloposcopy, endocervical curettage, and endometrial biop ... | 2000 | 11077238 |
| human papillomavirus infection of non-melanoma skin cancers in immunocompetent hosts. | human papilloma viruses (hpvs) consist of more than 70 different types and are known to be associated with numerous malignant tumors, including carcinomas of the mucosal and cutaneous epithelium. non-melanoma skin carcinoma (nmsc) is the most frequently occurring malignancy worldwide in the caucasian population. most studies examining the involvement of papillomaviruses in the development of cutaneous carcinomas have been performed on lesions from patients with epidermodysplasia verruciformis or ... | 2000 | 11078916 |
| the human papillomavirus e7 protein is able to inhibit the antiviral and anti-growth functions of interferon-alpha. | it is now well recognized that cervical cancer is caused by infection with certain human papillomavirus (hpv) subtypes and while interferon-alpha (ifn-alpha) is used to treat hpv-infected lesions, hpv appears to have developed a means to avoid the effects of ifn-alpha. clinically, resistance appears to be associated with the expression of the e7 oncoprotein. here we investigated the effects of expression in cells of the e7 protein from high- and low-risk papillomavirus subtypes on a range of res ... | 2000 | 11080488 |
| regular disappearance of the human papillomavirus genome after conization of cervical dysplasia by carbon dioxide laser. | we wished to evaluate the effectiveness of treatment of cervical dysplasia by laser conization in relation to persistence of human papillomavirus after treatment. | 2000 | 11084572 |
| overview of vaccinology with special reference to papillomavirus vaccines. | viruses that belong to six different families are a significant cause for neoplasia in man and animals. among them are the papillomaviruses that cause uterine cervical cancer in women. efforts to develop prophylactic vaccines against viruses that cause cancer are now a major research engagement. vaccinology, the science of vaccines, engages the sciences of immunology and of microbiology, both relying heavily on molecular biology. successful development of vaccines relies on extensive knowledge o ... | 2000 | 11091151 |
| preventing genital infections with human papillomavirus: lessons learned from the hiv epidemic. | despite the major differences in the clinical epidemiology of hiv and hpv infections, several lessons learned from the hiv epidemic may be relevant to future prevention of hpv infection. there has been a paradigm shift in conceptualizing the epidemiology of stds; targets of prevention now include: (1) determinants of exposures of susceptibles to infectious persons; (2) the efficiency of transmission; and (3) the duration of infectiousness. lessons learned that may be potentially relevant to hpv ... | 2000 | 11091152 |
| prospects for phase iii-iv hpv vaccination trials in the nordic countries and in estonia. | oncogenic, i.e. high risk human papillomavirus (hrhpv) types are the major cause of invasive cervical cancer (icc). putatively licensable vaccines against the hrhpvs have been developed and are approaching clinical phase iii trials that use persistent hpv infection as end point. direct extension of the phase iii trials towards long-term end points (icc and its immediate precursors: carcinoma in situ and severe dysplasia, i.e. cervical intraepithelial neoplasia grade iii, ciniii) is important, to ... | 2000 | 11091154 |
| the use of molecular techniques for the diagnosis and epidemiologic study of sexually transmitted infections. | molecular diagnostic tests are more sensitive and, in many cases, more specific than conventional laboratory methods for the detection of sexually transmitted infections. here, we review recently developed molecular methods for the diagnosis and subtyping of the most common sexually transmitted infections: infections caused by chlamydia trachomatis, neisseria gonorrhoeae, human papillomavirus, trichomonas vaginalis, and the agents of genital ulcer disease (haemophilus ducreyi, herpes simplex vir ... | 2000 | 11095835 |
| vulvar vestibulitis syndrome: a post-infectious entity? | vulvar vestibulitis syndrome, a condition characterized by inflammation of the vaginal introitus, causes chronic vulvar pain, particularly with intercourse. it occurs in at least 15% of women with chronic vulvovaginal symptoms, and it is a common cause of sexual dysfunction and resulting comorbidities. because 80% of women with vulvar vestibulitis syndrome describe an acute onset of symptoms, an infectious etiology has been suspected but never proven. initially, human papillomavirus infection wa ... | 2000 | 11095904 |
| frequency and spectrum of hpv types detected in cutaneous squamous-cell carcinomas depend on the hpv detection system: a comparison of four pcr assays. | the association of human papillomavirus (hpv) with cutaneous squamous-cell carcinomas (sccs) has been described recently, but the frequency and spectrum of hpv types identified differed substantially in distinct studies. | 2000 | 11096190 |
| major histocompatibility complex class ii polymorphisms and risk of cervical cancer and human papillomavirus infection in brazilian women. | infection with high-risk human papillomavirus (hpv) is the major risk factor for the development of malignant lesions in the uterine cervix. environmental, behavioral, and ill-defined genetic factors also have been implicated in the pathogenesis of this disease. associations between human leukocyte antigens (hlas) and cervical cancer, precursor lesions, and hpv infections have been reported in several populations. to verify whether hla-drb1, -dqa1, and -dqb1 diversity is related to cervical canc ... | 2000 | 11097225 |
| the planning of cervical cancer screening programmes in eastern europe: is viral testing a suitable alternative to smear testing? | cervical cancer screening with human papillomavirus (hpv) dna testing has potential advantages over conventional, smear testing in that it can predict cases in which invasive cancers are more likely to develop, may be cheaper to implement and improve compliance. in areas of the world where little formalized cervical cancer screening takes place, or where health resources are limited, hpv testing has been suggested as a possible alternative for primary screening. in this paper we demonstrate the ... | 2000 | 11105418 |
| detection and quantitation of human papillomavirus (hpv) dna in the sera of patients with hpv-associated head and neck squamous cell carcinoma. | the human papillomavirus (hpv) has been implicated as an etiological factor in a subset of head and neck squamous cell carcinoma (hnscc). because circulating tumor dna has previously been detected in the sera of patients with advanced hnscc (stage iii or iv), we hypothesized that hpv dna might be present in the sera of hpv-positive hnscc patients. serum dna extracts from 70 patients with hnscc were screened for hpv using conventional pcr and a real-time quantitative assay. all samples subjected ... | 2000 | 11106228 |
| resolution of recalcitrant human papillomavirus gingival infection with topical cidofovir. | cidofovir, a purine nucleotide analogue of cytosine, is a promising new drug that acts against a wide number of dna viruses. in 1997, the food and drug administration approved intravenous cidofovir for the treatment of cytomegalovirus retinitis in patients with acquired immunodeficiency syndrome. recent studies have shown cidofovir (1% gel or cream) to be effective for the treatment of recalcitrant and unmanageable viral cutaneous lesions induced by herpes, pox, and papillomavirus families. we r ... | 2000 | 11113816 |
| deletion of the fhit gene in neoplastic and invasive cervical lesions is related to high-risk hpv infection but is independent of histopathological features. | the fragile histidine triad (fhit) gene encompasses the common chromosomal fragile site fra3b. human papilloma virus (hpv), which is the main aetiological agent in cervical cancers, has been found to be able to integrate its genes into the chromosome 3 fragile site of cultured cells, deleting a piece of dna which includes the fhit gene. eighty-six microdissected archival cervical lletz biopsies comprising cases of cervical intraepithelial neoplasia (cin) 1 (n=27), cin3 (n=30) and microinvasive c ... | 2000 | 11113868 |
| characterization of adriamycin-induced g2 arrest and its abrogation by caffeine in fl-amnion cells with or without p53. | we investigated the effect of adriamycin on fl-amnion (fl) cells. after treatment with the drug, the cells arrested at g2, but we did not detect an increase in the p21 levels. we established a p53-deficient derivative of these cells, in which g2 arrest also occurred after treatment with adriamycin, suggesting that the arrest we observed in these cells is independent of the p53 pathway. low doses of adriamycin (100-200 ng/ml) induced g2 arrest, while late s-phase arrest was observed at high doses ... | 2001 | 11120603 |
| in vitro models to study cellular differentiation and function in human prostate cancers. | in vitro models to study cellular differentiation and function in human prostate cancers. to augment the currently available models of human prostate cancer in vitro, we have established extended life-span epithelial cultures from biopsies of well-differentiated prostate cancers. the genetic identity of the target cells was assessed by allelotyping, using microsatellites located on chromosome 8p, and microdissection of tissues and primary cell cultures. cells with an extended life span (pxe6) we ... | 2001 | 11121225 |
| oestrogen and progesterone increase the levels of apoptosis induced by the human papillomavirus type 16 e2 and e7 proteins. | human papillomavirus (hpv) type 16 infects the genital tract and is generally acknowledged to be a causative agent of cervical cancer. hpv infection alone is not sufficient to induce cervical cancer and other factors such as steroid hormones are thought to play a role in the establishment and/or progression of this disease. the hpv-16 e2 protein is required for virus replication and modulates viral gene expression whereas the hpv-16 e7 protein is required for cell transformation. we and others h ... | 2001 | 11125173 |
| degradation of the e7 human papillomavirus oncoprotein by the ubiquitin-proteasome system: targeting via ubiquitination of the n-terminal residue. | the e7 oncoprotein of the high risk human papillomavirus type 16 (hpv-16), which is etiologically associated with uterine cervical cancer, is a potent immortalizing and transforming agent. it probably exerts its oncogenic functions by interacting and altering the normal activity of cell cycle control proteins such as p21waf1, p27kip1 and prb, transcriptional activators such as tbp and ap-1, and metabolic regulators such as m2-pyruvate kinase (m2-pk). here we show that e7 is a short-lived protein ... | 2000 | 11127826 |
| the influence of cotinine on interleukin 6 expression in smokers with cervical preneoplasia. | several epidemiological investigations have shown that cigarette smoking leads to increased serum il-6 levels and is a risk factor for cervical cancer. | 2000 | 11130096 |
| tracing human papillomavirus dna in nasal polyps by polymerase chain reaction. | human papillomavirus (hpv) infections are related to the genesis of various benign and malignant human neoplasias. the hpv types 16 and 18 seem to be causally related to the development of most squamous cell carcinoma of the anogenital tract and a proportion of carcinomas of the upper aerodigestive tract. the near 100% positivity of the hpv types 6 and 11 in laryngeal papillomatosis is well established. we investigated whether hpv also plays a role in non-neoplastic mucosal entities such as sinu ... | 2000 | 11132723 |
| the comparison of the effect of ltr72 and mf59 adjuvants on mouse humoral response to intranasal immunisation with human papillomavirus type 6b (hpv-6b) virus-like particles. | infections with genital human papillomaviruses (hpv) are likely to be neutralised more efficiently if a mucosal immune response can be elicited at the viral entry site. local iga antibodies are highly induced when antigens are co-administered with mucosal adjuvants, such as cholera toxin (ct) and escherichia coli heat labile enterotoxin (lt) which, however, are not expected to have wide application because of their pronounced toxicity. we have immunised mice intranasally with hpv-6b virus-like p ... | 2000 | 11137233 |
| mucosal vaccination with a recombinant salmonella typhimurium expressing human papillomavirus type 16 (hpv16) l1 virus-like particles (vlps) or hpv16 vlps purified from insect cells inhibits the growth of hpv16-expressing tumor cells in mice. | human papillomaviruses, mainly type 16 (hpv16), are responsible for cervical intraepithelial neoplasia, which can lead, in association with other factors, to cervical cancer. both salmonella recombinant vaccine strains assembling hpv16 virus-like particles (vlps) and hpv16 vlps purified from insect cells are able to induce hpv16 neutralizing antibodies in genital secretions of mice after nasal immunization. anti-hpv16-specific antibodies in cervical secretions of women may prevent genital infect ... | 2001 | 11145916 |
| impact of human papillomavirus infection on the expression of the kai1 metastasis suppressor protein in invasive cervical cancer. | downregulation of kai1 metastasis suppressor protein is associated with dismal prognosis in a variety of cancers. mutation of p53 was suggested to be involved in kai1-downregulation. in cervical cancer, p53 is inactivated by human papillomavirus (hpv) oncoprotein e6 with the grade of inactivation depending on the hpv type. kai1-expression was immunohistochemically determined in 67 specimens of cervical cancer, hpv-typing was performed using polymerase chain reaction (pcr), cloning, and sequencin ... | 2001 | 11146234 |
| verrucous carcinoma arising in hidradenitis suppurativa. | there are many reported cases of squamous carcinoma complicating hidradenitis suppurativa, but only one previous mention of verrucous carcinoma in this setting. we describe a case of verrucous carcinoma arising in hidradenitis suppurativa of the anal margin in a non-immunosuppressed man. this is the second report of verrucous carcinoma arising in a lesion of hidradenitis suppurativa. although hidradenitis suppurativa can involve multiple intertriginous sites, malignant degeneration occurs mostly ... | 2000 | 11151441 |
| human papillomavirus infection requires cell surface heparan sulfate. | using pseudoinfection of cell lines, we demonstrate that cell surface heparan sulfate is required for infection by human papillomavirus type 16 (hpv-16) and hpv-33 pseudovirions. pseudoinfection was inhibited by heparin but not dermatan or chondroitin sulfate, reduced by reducing the level of surface sulfation, and abolished by heparinase treatment. carboxy-terminally deleted hpv-33 virus-like particles still bound efficiently to heparin. the kinetics of postattachment neutralization by antiseru ... | 2001 | 11152531 |
| complete switch from mdm2 to human papillomavirus e6-mediated degradation of p53 in cervical cancer cells. | the e6 oncoprotein of human papillomaviruses (hpvs) that are associated with cervical cancer utilizes the cellular ubiquitin-protein ligase e6-ap to target the tumor suppressor p53 for degradation. in normal cells (i.e., in the absence of e6), p53 is also a target of the ubiquitin-proteasome pathway. under these conditions, however, p53 degradation is mediated by mdm2 rather than by e6-ap. here we show in a mutational analysis that, surprisingly, the structural requirements of p53 to serve as a ... | 2001 | 11158620 |
| specific recognition of four-way dna junctions by the c-terminal zinc-binding domain of hpv oncoprotein e6. | e6 is an oncoprotein implicated in cervical cancers produced by " high risk " human papillomaviruses. e6 binds specifically to several cellular proteins, including the tumour suppressor p53 and the ubiquitin ligase e6-ap. however, e6 is also a dna-binding protein which recognizes a structural motive present in four-way junctions. here, we demonstrate that the c-terminal zinc-binding domain of e6, expressed separately from the rest of the protein, fully retains the selective four-way junction rec ... | 2001 | 11162088 |
| nasal immunization of mice with peptide having a cross-neutralization epitope on minor capsid protein l2 of human papillomavirus type 16 elicit systemic and mucosal antibodies. | a common cross-neutralization epitope for human papillomavirus types 6 and 16 (hpv 6 and 16) is present in the region of amino acids (aa) 108-120 of hpv-16 minor capsid protein, l2. we nasally immunized balb/c mice with a synthetic peptide with the 13 aa hpv 16 l2 sequence, and examined the antibodies elicited. elisa showed that the immunization induced predominantly igg and iga antibodies cross-binding to l1/l2-capsids of hpvs 6, 16, and 18 in sera and in vaginal secretions, respectively. the s ... | 2001 | 11163673 |
| immunological analyses of human papillomavirus capsids. | recombinant human papillomavirus (hpv) virus-like particles (vlps) are promising vaccine candidates for controlling anogenital hpv disease. questions remain, however, concerning the extent of capsid antigenic similarity between closely related virus genotypes. to investigate this issue, we produced vlps and corresponding polyclonal immune sera from several anogenital hpv types, and examined these reagents in enzyme-linked immunosorbent assays (elisas) and in cross-neutralization studies. despite ... | 2001 | 11166904 |
| chlamydia trachomatis and human papillomavirus infection in indian women with sexually transmitted diseases and cervical precancerous and cancerous lesions. | sexually transmitted diseases (stds) and anogenital cancers are the major health problems in indian women but no reliable estimate of the prevalence of either genital chlamydial infection or human papillomavirus (hpv) infection in std patients is available. the aim of this study was to detect the frequency of chlamydia trachomatis and the most prevalent high-risk hpv type 16 (hpv 16) infection in indian women, with stds and precancerous and cancerous lesions of the uterine cervix by polymerase c ... | 2000 | 11168078 |
| in situ hybridization detection of single-copy human papillomavirus on isolated cells, using a catalyzed signal amplification system: genpoint. | the performance and drawbacks of genpoint, which is a catalyzed signal amplification system for immunohistochemistry, have been evaluated for its ability to reveal human papillomavirus (hpv) dna detected by in situ hybridization with biotinylated dna probes. for this aim, formalin-fixed cell deposits from carcinoma cells of the uterine cervix, caski, siha, and hela, containing, respectively, 600 copies of hpv dna type 16, 1-2 copies of hpv dna type 16, and 10-50 copies of hpv dna type 18, were u ... | 2001 | 11169889 |
| differential requirement for the stress-activated protein kinase/c-jun nh(2)-terminal kinase in rnadamage-induced apoptosis in primary and in immortalized fibroblasts. | onconase, an anticancer ribonuclease, damages cellular trna and causes caspase-dependent apoptosis in targeted cells (m. s. iordanov, o. p. ryabinina, j. wong, t. h. dinh, d. l. newton, s. m. rybak, and b. e. magun. cancer res. 60, 1983-1994, 2000). the proapoptotic action of onconase depends on its rnase activity, but the molecular mechanisms leading to rna damage-induced caspase activation are completely unknown. in this study, we have investigated whether onconase activates two signal-transdu ... | 2000 | 11170843 |
| human papillomavirus in the oral cavities of children and adolescents. | the purpose of this pilot study was to determine the frequency of human papillomavirus (hpv) in the oral cavities of children and adolescents and to identify potential risk factors for hpv infection. | 2001 | 11174573 |
| enhancement of sindbis virus self-replicating rna vaccine potency by targeting antigen to endosomal/lysosomal compartments. | self-replicating rna vaccines (rna replicons) have emerged as an attractive approach for tumor immunotherapy. rna replicons do not integrate into host chromosomes, eliminating the concern for oncogenicity associated with a dna vaccine. in this study, we used human papillomavirus type 16 (hpv-16) e7 as a model antigen and evaluated e7-specific immunity generated by a sindbis virus self-replicating rna vector, sin-rep5. three different constructs were created to target e7 antigen to different cell ... | 2001 | 11177561 |
| absence of human papillomavirus sequences in early stage prostate cancer. | an etiological role for human papilloma viruses (hpvs) has been proposed for a variety of malignancies, and especially in cervical carcinomas. the role of hpvs in prostate cancer remains controversial. in particular, using primers derived from the l1 gene of hpv, it has been suggested that hpv sequences are associated with prostate malignancies. pcr and southern blot hybridization was used to detect the presence of hpv dna in fresh tissue from patients undergoing radical prostatectomy for prosta ... | 1999 | 11180776 |
| cervical carcinoma and human papillomavirus: on the road to preventing a major human cancer. | 2001 | 11181763 | |
| immunotherapy of a human papillomavirus type 16 e7-expressing tumor by administration of fusion protein comprised of mycobacterium bovis bcg hsp65 and hpv16 e7. | human papillomavirus type 16 (hpv16) infection has been linked to the development of cervical and anal dysplasia and cancer. one hallmark of persistent infection is the synthesis of the viral e7 protein in cervical epithelial cells. the expression of e7 in dysplastic and transformed cells and its recognition by the immune system as a foreign antigen make it an ideal target for immunotherapy. utilizing the e7-expressing murine tumor cell line, tc-1, as a model of cervical carcinoma, an immunother ... | 2000 | 11189443 |
| immunological and viral factors associated with the response of vulval intraepithelial neoplasia to photodynamic therapy. | topical 5-aminolevulinic acid-based photodynamic therapy (pdt) has produced complete response rates of >90% for nonmelanoma skin carcinomas, which are mostly human papillomavirus (hpv) negative. using a similar treatment protocol, we observed a short-term response in only one third (10 of 32) of high-grade vulval intraepithelial neoplasia (vin 2-3) lesions. unifocal lesions were found more responsive than multifocal and pigmented lesions. animal model studies have suggested that long-term pdt re ... | 2001 | 11196160 |
| oral condyloma acuminatum. | oral condyloma acuminatum is a papillomatous lesion that is transmitted sexually and associated with the human papilloma virus. condyloma acuminatum generally appears one to three months after exposure to an infected partner and presents in multiple form in the oral cavity. | 2000 | 11199556 |
| human papillomavirus-11-associated recurrent respiratory papillomatosis is more aggressive than human papillomavirus-6-associated disease. | the aim of this study was to determine whether viral type (hpv-6 vs. hpv-11) could predict the clinical course of recurrent respiratory papillomatosis in children. viral typing, using the polymerase chain reaction, was performed on laryngeal biopsies of 61 patients treated at children's hospital of michigan. hpv-6 was detected in 29 of the patients' biopsies and hpv-11 in 32 biopsies. hpv-11 was more common among the african-american patients than among caucasians (p = 0.001). patients with hpv- ... | 2001 | 11200493 |
| tonsillar and other upper aerodigestive tract cancers among cervical cancer patients and their husbands. | the study aimed at probing the possible role of human papillomavirus (hpv) infection in squamous cell carcinomas of the upper aerodigestive tract, with a special reference to tonsillar cancer. we used the swedish family cancer database to analyse second cancers in the upper aerodigestive tract of women first diagnosed with in-situ or invasive cervical cancer. first cancers of their husbands were also analysed. standardized incidence ratios (sirs) were calculated for female and male cancers, adju ... | 2000 | 11201683 |
| squamous cell carcinoma of the nail bed: is finger predominance another clue to etiology? a report of 5 cases. | although squamous cell carcinoma (scc) is commonly found on sun-exposed skin, the occurrence of this malignancy in the nail bed is rare. we report 5 cases of scc of the nail bed and suggest that the disproportionate number of neoplasms of this type on the second, third, and fourth fingers, combined with the known relationship of scc and human papillomavirus (hpv), is evidence that most scc of the nail bed result from contact with hpv. moreover, we suggest that patients who present with new, verr ... | 2001 | 11204607 |
| hpv testing: where are we now? | 1) to assess if hpv (human papillomavirus)-dna testing using the presently available technology offers any advantage over the utilization of the traditional pap-smear as a screening tool for women at risk for cervical pre-cancers. 2) to assess if the hpv-dna test is a valuable intermediate triage method for patients with pap-smears demonstrating ascus (abnormal squamous cells of undetermined significance) or lg-sil (low-grade squamous intraepithelial lesions) in order to better select those pati ... | 2000 | 11205199 |
| angiogenesis and vasodilation in skin warts. association with hpv infection. | angiogenesis is required for tumour growth. since human papillomavirus (hpv) infection is associated with the development of neoplastic lesions, the aim of this study was to determine the possible association between hpv infection and angiogenesis in benign tumours. | 2000 | 11205298 |
| high-risk and multiple human papillomavirus infections associated with cervical abnormalities in japanese women. | to estimate the risk of human papillomavirus (hpv) infection for cervical malignancies, we conducted a case-control study in japan. abnormal cervical cell (366) and normal cell samples (1562) were tested for the presence of hpv dna using a new pcr-based test (lcr-e7 pcr). when single hpv infections were considered, 26 different hpv types were identified in normal cervices and in low-grade squamous intraepithelial lesions (lsil); whereas hpv-16, -18, -31, -33, -35, -45, -51, -52, -56, -58 and -67 ... | 2001 | 11205488 |
| morphologic conversion of a neuroblastoma-derived cell line by e6-mediated p53 degradation. | neuroblastoma-derived tumor cells, unlike cells from other tumor types, characteristically express a wildtype but cytoplasmically sequestered p53 protein. to ascertain whether the p53 in these cells retained any physiological activity, we inactivated it in sk-n-sh cells, a neuroblastoma-derived cell line, by introducing the human papilloma virus type 16 e6 expression plasmid. parent sk-n-sh cell cultures are composed of two cell types exhibiting characteristic morphologies designated neuroblasti ... | 2001 | 11205742 |
| high prevalence of high grade squamous intraepithelial lesions and microinvasive carcinoma in women with a cytologic diagnosis of low grade squamous intraepithelial lesions. | to evaluate the histologic nature of low grade intraepithelial lesion (lsil) in a region with a high prevalence of invasive cervical carcinoma and to propose a management protocol. | 2001 | 11209634 |
| p53 codon 72 polymorphism and various human papillomavirus 16 e6 genotypes are risk factors for cervical cancer development. | risk factors other than human papillomavirus (hpv) infection per se for cervical cancer development have been investigated recently. it was suggested that hpv 16 e6 variants and the p53 codon 72 arginine polymorphism could be progression markers. indeed, it has been demonstrated that specific e6 variants and p53 arginine were both enriched in cancer. however, especially with regard to the latter, divergent results have been reported. our aim was thus to investigate whether p53 arginine is import ... | 2001 | 11212257 |
| [cancer and prevention]. | the aim of the study was to estimate the preventable potential of various types of cancer in denmark on the basis of present knowledge. | 2001 | 11218778 |
| high-risk human papillomavirus is sexually transmitted: evidence from a follow-up study of virgins starting sexual activity (intercourse). | genital human papillomavirus (hpv) infection is generally considered to be sexually transmitted. however, nonsexual spread of the virus has also been suggested. the goal of this study was to assess: (a) the role of sexual intercourse in the transmission of hpv; (b) the determinants for seroconversion; and (c) the correlation between hpv dna, abnormal cervical cytology, and serological response to hpv16. one hundred virgins and 105 monogamous women were randomly selected from a population-based c ... | 2001 | 11219765 |
| carcinosarcoma of the uterine cervix: a report of eight cases with immunohistochemical analysis and evaluation of human papillomavirus status. | carcinosarcomas (malignant müllerian mixed tumors [mmmts]) of the uterine cervix are rare neoplasms. this report describes the morphology, immunohistochemical profiles, and human papillomavirus (hpv) status of eight cervical mmmts. patients' ages ranged from 32 to 93 years (mean, 61 years). seven cases showed in situ squamous cell carcinoma (scc). the invasive epithelial component (ec) was composed of combined adenoid basal carcinoma, basaloid scc, and adenoid cystic carcinoma (acc) in two cases ... | 2001 | 11224604 |
| metastatic mhc class i-negative mouse cells derived by transformation with human papillomavirus type 16. | in the endeavour to develop a model for studying gene therapy of cancers associated with human papillomaviruses (hpvs), mouse cells were transformed with the hpv type 16 (hpv16) and activated h-ras oncogenes. this was done by cotransfection of plasmid p16hhmo, carrying the hpv16 e6/e7 oncogenes, and plasmid pej6.6, carrying the gene coding for human h-ras oncoprotein activated by the g12v mutation, into secondary c57bl/6 mouse kidney cells. an oncogenic cell line, designated mk16/1/iiiabc, was d ... | 2001 | 11225590 |
| validation of array-based gene expression profiles by real-time (kinetic) rt-pcr. | we evaluated real-time (kinetic) reverse transcription-polymerase chain reaction (rt-pcr) to validate differentially expressed genes identified by dna arrays. gene expression of two keratinocyte subclones differing in the physical state of human papillomavirus (episomal or integrated) was used as a model system. high-density filter arrays identified 444 of 588 genes as either negative or expressed with less than twofold difference, and the other 144 genes as expressed uniquely or with more than ... | 2001 | 11227069 |
| the role of human papillomavirus deoxyribonucleic acid assay and repeated cervical cytologic examination in the detection of cervical intraepithelial neoplasia among human immunodeficiency virus-infected women. cervical disease study group of the american foundation for aids research community based clinical trials network. | we sought to measure the characteristics of a quantitative human papillomavirus deoxyribonucleic acid assay and repeated cervical cytologic examination in screening for cervical intraepithelial neoplasia among human immunodeficiency virus-infected women. | 2001 | 11228481 |
| isolation, immortalization, and initial characterization of uterine cell lines: an in vitro model system for the porcine uterus. | the aim of this study was to develop immortalized cell lines from porcine uterus. endometrial cells including luminal epithelium (le), glandular epithelium (ge), stroma (st), and myometrium (myo) were enzymatically isolated from the uterus of a day 12 pregnant gilt. primary cultures were immortalized by transduction with a retroviral vector containing the e6 and e7 open reading frames of human papillomavirus type 16 (lxsn-16e6e7) packaged by the amphotropic fibroblast line pa-317. cells having i ... | 2000 | 11229597 |
| comparison of information processing technologies. | to examine the type of information obtainable from scientific papers, using three different methods for the extraction, organization, and preparation of literature reviews. | 2001 | 11230385 |
| transitional cell carcinoma of the endometrium associated with a benign ovarian brenner tumor: a case report. | transitional cell carcinomas (tccs) of the endometrium are rare, and only 10 cases have been described to date. we report the case of a 46-year-old woman who developed both a tcc of the endometrium and a benign ovarian brenner tumor. such an association has not yet been reported in the literature. immunohistochemical studies of the uterine tumor showed cytokeratin 7 positivity and cytokeratin 20 negative staining, which was consistent with a müllerian derivation. human papilloma virus (hpv) immu ... | 2001 | 11230712 |
| [the role of human papillomavirus in cyto-histological practice: distribution and prevalenceof hig-risk strains (16, 18, 31, 33, and 35) in intraepithelial lesions and neoplasia of the uterine cervix]. | many studies have already shown the association of persistent infection of human high risk papillomavirus (hpv) with the development of pre-invasive and invasive cervical disease. | 2000 | 11234302 |
| schneiderian papillomas and carcinomas: a review. | the ectodermally derived schneiderian mucosa gives rise to an extremely varied collection of benign and malignant neoplasms. prototypical of these are the schneiderian papillomas (inverted, fungiform, and cylindrical cell) and their malignant counterparts. human papilloma virus (hpv) is currently the leading candidate as a cofactor in the pathogenesis of the papillomas. carcinomas arise in association with the papillomas in about a 10% frequency. the carcinomas may be synchronous or metachronous ... | 2001 | 11236954 |
| cell-mediated immune response to human papillomavirus infection. | 2001 | 11238198 | |
| human papillomavirus dna detection in sperm using polymerase chain reaction. | to detect human papillomavirus (hpv) in semen and find if sperm washing removes hpv dna. | 2001 | 11239636 |
| the use of human papillomavirus typing in detection of cervical neoplasia in recife (brazil). | high risk types of human papillomavirus (hr-hpv) play a major role in cervical cancer oncogenesis. this study aims to evaluate the efficacy of hpv detection and typing as a means of identifying cervical neoplasia in a high risk population. a management algorithm for implementation of hpv detection in clinical practice is also proposed. a nested case-control within a cohort study was undertaken in recife (brazil). all 479 participants had cervical scrapes collected for hpv detection followed by c ... | 2000 | 11240666 |
| infection and cervical intraepithelial neoplasia. | the impact of sexually transmitted diseases (stds) on the development of cervical intraepithelial neoplasia (cin) has been increasingly recognized over the last 20 years. much attention has been focused on human papillomavirus (hpv) and the potential for screening for certain hpv types alongside standard cervical cytology in the hope of identifying those females at particular risk of developing high grade cin or invasive carcinoma. some infections, for example herpes simplex virus (hsv), have be ... | 1999 | 11240764 |
| human papillomavirus variants and squamous neoplasia of the cervix. | human papillomaviruses (hpvs) play a central role in the aetiology of cervical neoplasia. however, only a small proportion of cervical intraepithelial lesions infected with high-risk hpvs will progress to invasive cervical carcinoma, which indicates the involvement of additional factors. an important emerging viral factor is naturally occurring intratypic sequence variation. such variation has been used to study the geographical spread of hpvs, but there is increasing evidence that it may be imp ... | 2001 | 11241407 |
| detection of genetic changes in anal intraepithelial neoplasia (ain) of hiv-positive and hiv-negative men. | compared with hiv-negative individuals, hiv-positive individuals have a higher prevalence of anogenital human papillomavirus (hpv) infection, as well as a higher incidence of hpv-associated anal cancer. little is currently known of chromosomal changes occurring in anal intraepithelial neoplasia (ain), the probable precursor to anal cancer. genetic changes in ain were characterized by comparative genomic hybridization (cgh) in a study of samples obtained from 19 hiv-positive and 11 hiv-negative m ... | 2001 | 11242198 |
| human papillomavirus dna in plasma of patients with cervical cancer. | human papillomavirus (hpv) is a crucial etiological factor for cervical cancer (cc) development. from a diagnostic view-point, the consistent presence of hpv in cc allows the viral dna to be used as a genetic marker. the aims of this study were to evaluate the presence, physical status and clinical significant of hpv dna in circulation of cc patients. | 2001 | 11244579 |
| [human papillomavirus typing in routine cervical smears. results from a series of 3778 patients]. | as human papillomavirus (hpv) infection is a significant risk factor for cervical cancer, the aim of this study was to assess the efficacy of hpv detection techniques in predicting the presence or the development of a high-grade squamous intra epithelial lesion. additional reasons for carrying out this investigation were as follows. it was considered that the clinical value of hpv typing may have been underestimated in certain previous reports, which based their findings on first-generation assa ... | 2000 | 11244634 |
| associations between smoking and adenocarcinomas and squamous cell carcinomas of the uterine cervix (united states). | few studies of smoking and cervical carcinoma have addressed the rare cervical adenocarcinomas or used dna-based tests to control for human papillomavirus (hpv) infection. | 2001 | 11246844 |