Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| [anatomico-surgical contribution to the knowledge of the lymphatic spread of gastric adenocarcinoma]. | following the researches performed on lymphatic spread of gastric carcinoma, our study aimed to add useful informations to establish the extension of exeresis with radical intent on the basis of recent studies on sentinel node mapping. the study includes 120 consecutive cases of gastric cancer operated between 1996 and 2000. sixty-nine (57.5%) males and 51 (42.5%) females, with age ranging between 29 and 84 years, were evaluated. cancer location was in 17 patients upper third, in 35 middle third ... | 2002 | 12192917 |
| thoracoscopy/laparoscopy in the staging of esophageal cancer: maryland experience. | precise clinical staging of esophageal cancer before treatment is important. thoracoscopic/laparoscopic (ts/ls) staging has been proposed as a promising staging method. this study was conducted to evaluate the potential benefits of ts/ls staging over conventional noninvasive clinical staging in patients with esophageal cancer. from 1991 to 1999, 111 patients with esophageal cancer underwent ts/ls staging by the university of maryland medical system. pretreatment staging workup included computed ... | 2002 | 12193812 |
| [post-partum autoimmune thyroiditis in a patient presenting with sheehan's syndrome]. | a 30-year-old women developed post-partum thyroiditis associated with post- partum pituitary infarction. pituitary stimulation tests demonstrated global pituitary deficiency but there was no thyrotropin response to trh stimulation and free t4 was normal. associated goiter and high circulating levels of microsomal antibodies led to the diagnosis of post-partum thyroiditis associated with pituitary deficiency. two cases of this unusual association have been reported. the underlying pathogenesis mi ... | 2002 | 12193878 |
| the problem of goitre with particular consideration of goitre resulting from iodine deficiency (ii): management of non-toxic nodular goitre and of thyroid nodules. | the present opinions on the therapy with l-thyroxine (l-t4) of non-toxic multinodular, as well as of non-toxic thyroid nodules are rather divergent. this treatment is based on the suppression of tsh secretion from the pituitary. there are no doubts that fine-needle aspiration biopsy (fnab) performance is the first and--at the same time--the most significant diagnostic procedure in the case of thyroid structural lesions (nodules, goitre, thyroiditis). fnab performance should--by all means--preced ... | 2002 | 12195241 |
| multiple variations in the azygos venous system: a preaortic interazygos vein and the absence of hemiazygos vein. | multiple variations of the azygos venous system were detected during routine dissection. the hemiazygos vein was underdeveloped. on the left side of the thorax, posterior intercostal veins between the 8th and 11th intercostal spaces and the subcostal vein drained into the azygos vein independently. in addition, the posterior 4th, 5th, 6th and 7th intercostal veins united and formed two superior and inferior trunks. the superior common trunk, at the level of the t4 vertebra, crossed the vertebral ... | 2002 | 12197015 |
| nanocluster with one missing core atom: a three-dimensional hybrid superlattice built from dual-sized supertetrahedral clusters. | in addition to having size-dependent properties, nanoclusters also behave like large pseudo-atoms and serve as building blocks for the construction of superlattices with properties that differ from either individual clusters or the bulk. while colloidal nanoparticles usually self-assemble into close-packed lattices, supertetrahedral clusters that are regular fragments of the zinc blende-type lattice can form covalent lattices with large pore size and high pore volume. unfortunately, the size of ... | 2002 | 12197715 |
| bispectral index in patients with target-controlled or manually-controlled infusion of propofol. | in this prospective, randomized study we compared bispectral index (bis), hemodynamics, time to extubation, and the costs of target-controlled infusion (tci) and manually-controlled infusion (mci) of propofol. forty patients undergoing first-time implantation of a cardioverter-defibrillator were included. anesthesia was performed with remifentanil (0.2-0.3 micro g. kg(-1). min(-1)) and propofol. propofol was used as tci (plasma target concentration, 2.5-3.5 micro g/ml; n = 20) or mci (3.0-4.0 mg ... | 2002 | 12198052 |
| crystallization and preliminary crystallographic study of a ternary complex between the t4 phage beta-glucosyltransferase, uridine diphosphoglucose and a dna fragment containing an abasic site. | a base-flipping phenomenon has been established for dna methyltransferases and for dna base-excision repair glycosylases and is likely to prove general for enzymes that need access to dna bases to undergo chemical reaction. t4 phage beta-glucosyltransferase (bgt) is a good candidate for this novel mechanism. in order to confirm this, bgt was crystallized with an abasic site-containing dna and uridine diphosphoglucose (udp-glucose). the crystallization strategy is described. a complete data set w ... | 2002 | 12198310 |
| periodontal 5'-deiodination on forced-induced root resorption--the protective effect of thyroid hormone administration. | the present investigation was designed to study the protective effect given by thyroid hormone (th) on root resorption: (1) whether intra-peritoneal versus oral th administration had the same efficiency; and (2) whether this effect involved local or systemic mechanisms. for this purpose, circulating t3 levels, systemic alkaline phosphatase (apase) activity, and 5'deiodinase (5'd) activity were evaluated in the periodontal area of 80 sprague-dawley rats, 8 weeks of age, in which orthodontic appli ... | 2002 | 12198866 |
| iodine and selenium deficiency in school-children in an endemic goiter area in turkey. | endemic goiter is one of the most important health problems in turkey. however, there are not enough studies associated with iodine and selenium status. this study was carried out to establish the effects of iodine and selenium levels on thyroid gland size and thyroid functions in 73 healthy school-children, 7-12 years old (mean 9.56 +/- 1.77 years), 38 girls (52%) and 35 boys (48%), living in an endemic goiter area. goiter was found in 32 of the children (43.8%) by palpation, and 56 of the chil ... | 2002 | 12199330 |
| linear growth in children with congenital hypothyroidism detected by neonatal screening and treated early: a longitudinal study. | we performed a longitudinal study of a cohort of 74 children with congenital hypothyroidism (ch) detected by neonatal screening (buenos aires province, argentina) up to the age of 3 years old, in order to study linear growth and the relationship with the severity of ch at diagnosis. the mean age at diagnosis and the start of the treatment was 16.9 +/- 5.2 days. the patients were divided into group 1--severe ch (pretreatment t4 level <4 microg/dl) (n = 47)--and group 2--less severe ch (pretreatme ... | 2002 | 12199341 |
| follow up of oesophageal cancer therapy at the kenyatta national hospital, nairobi. | to determine the pattern of follow-up for oesophageal cancer patients following hospital discharge and reviewing followup results of the different treatment modalities with emphasis on oesophagectomies. | 2001 | 12199447 |
| effects of sodium nitroprusside-induced controlled hypotension on pancreatic function assessed by pancreatitis-associated protein in patients undergoing radical prostatectomy. | controlled hypotension may alter organ blood flow and tissue oxygenation. the aim of the study was to investigate whether induced hypotension using sodium nitroprusside alters pancreatic function assessed by pancreatitis-associated protein concentrations in the blood. | 2002 | 12200953 |
| prognostic value of mandibular involvement in oral cavity cancers. | the aim of the study was to determine, in a homogeneous population, the prognostic impact of mandibular involvement in oral cancers. | 2002 | 12201006 |
| rejoining of dna strand breaks by t4 dna ligase in mammalian cells. | we have tested the ability of t4 dna ligase to rejoin radiation-induced dna strand breaks in living hamster cells (cho-k1, em9, xrs-5). t4 dna ligase was introduced into cells by electroporation prior to x-irradiation. single- and double-strand breaks were measured by the alkaline comet assay technique, and double-strand breaks (dsbs) were evaluated by the pulsed-field gel electrophoresis method. in the comet assay, the three cell lines showed reduced tail moments following pretreatment with t4 ... | 2002 | 12201059 |
| treatment and therapeutic monitoring of canine hypothyroidism. | thirty-one dogs with spontaneous hypothyroidism were treated with thyroid hormone replacement therapy (thrt) and monitored for approximately three months. good clinical and laboratory control was ultimately achieved in all cases with a mean l-thyroxine (t4) dose of 0.026 mg/kg administered once daily. there was a significant increase and decrease in circulating total t4 and canine thyroid stimulating hormone (ctsh) concentrations, respectively, after starting thrt. after commencing treatment, 11 ... | 2002 | 12201441 |
| neonatal thyrotoxicosis and maternal infertility in thyroid hormone resistance due to a mutation in the trbeta gene (m313t). | we report two unusual cases of resistance to thyroid hormone (rth) in one family. the first case, a male infant, had clinical features of thyrotoxicosis in the neonatal period. in the fourth week of life weight gain was poor despite a daily intake of standard infant formula almost double the infant's estimated requirements. at this time serum free t4 (ft4) was 60.7 pmol/l (normal range [nr] 11-25 pmol/l) and tsh was inappropriately normal at 1.8 mu/l (nr 0.3-4.0 mu/l). the infant responded clini ... | 2002 | 12201835 |
| microarray analysis of gene expression during bacteriophage t4 infection. | genomic microarrays were used to examine the complex temporal program of gene expression exhibited by bacteriophage t4 during the course of development. the microarray data confirm the existence of distinct early, middle, and late transcriptional classes during the bacteriophage replicative cycle. this approach allows assignment of previously uncharacterized genes to specific temporal classes. the genomic expression data verify many promoter assignments and predict the existence of previously un ... | 2002 | 12202221 |
| electrostatic contributions to t4 lysozyme stability: solvent-exposed charges versus semi-buried salt bridges. | we carried our poisson-boltzmann (pb) calculations for the effects of charge reversal at five exposed sites (k16e, r119e, k135e, k147e, and r154e) and charge neutralization and proton titration of the h31-d70 semi-buried salt bridge on the stability of t4 lysozyme. instead of the widely used solvent-exclusion (se) surface, we used the van der waals (vdw) surface as the boundary between the protein and solvent dielectrics (a protocol established in our earlier study on charge mutations in barnase ... | 2002 | 12202359 |
| problems related to tnm staging: patients with stage iii non-small cell lung cancer. | many reports have raised certain problems concerning the current tnm classification of lung cancer, namely that there is no sufficient difference in prognosis between patients with pathologic stage iiia and iiib disease. for clarifying this problem, the present study was constructed in light of t3 and t4 classifications. | 2002 | 12202867 |
| feasibility of induction chemotherapy using bronchial arterial infusion for locally advanced non-small cell lung cancer: a pilot study. | we examined the feasibility and effectiveness of bronchial arterial infusion (bai) as induction chemotherapy before surgery for locally advanced non-small cell lung cancer (nsclc). | 2002 | 12203053 |
| free thyroxine levels during the first weeks of life and neurodevelopmental outcome until the age of 5 years in very preterm infants. | we have conducted a randomized trial with thyroxine (t4) in 200 infants <30 weeks' gestation. t4 treatment was associated with better 5-year outcome in infants <29 weeks' gestation, but with worse outcome in infants of 29 weeks. these effects could be related to low, respectively high free thyroxine (ft4) levels | 2002 | 12205256 |
| deoxyribonucleotide synthesis in anaerobic microorganisms: the class iii ribonucleotide reductase. | for growth under oxygen-free atmosphere, some strict or facultative anaerobes depend on a class iii ribonucleotide reductase for the synthesis of deoxyribonucleotides, the dna precursors. prototypes for this class of enzymes are ribonucleotide reductases from escherichia coli and bacteriophage t4. this review article describes their structural and mechanistic properties as well as their complex allosteric regulation. their evolutionnary relationship to class i and class ii ribonucleotide reducta ... | 2002 | 12206460 |
| the mechanism of transcriptional activation by the topologically dna-linked sliding clamp of bacteriophage t4. | three viral proteins participate directly in transcription of bacteriophage t4 late genes: the sigma-family protein gp55 provides promoter recognition, gp33 is the co-activator, and gp45 is the activator of transcription; gp33 also represses transcription in the absence of gp45. transcriptional activation by gp45, the toroidal sliding clamp of the t4 dna polymerase holoenzyme, requires assembly at primer-template junctions by its clamp loader. the mechanism of transcriptional activation has been ... | 2002 | 12206760 |
| twice-a-day radiotherapy for head and neck cancer: the catalan institute of oncology experience. | the purpose of this work is to evaluate the contribution of hyperfractionated radiotherapy (rt) in head and neck cancer by sub-localisation. | 2002 | 12208570 |
| neoadjuvant chemotherapy followed by salvage surgery: effect on survival of patients with primary noncurative gastric cancer. | the prognosis for gastric cancer patients who undergo noncurative resection is extremely poor. this study evaluated the effects of neoadjuvant chemotherapy for primary noncurative gastric cancer. thirty-four patients with biopsy-proven noncurative gastric cancer were treated with either of two neoadjuvant chemotherapies: femtxp (5-fluorouracil, epirubicin, methotrexate, cisplatin) or thp-flpm (pirarubicin, 5-fluorouracil, leucovorin, cisplatin, mitomycin c). noncurability was determined by conve ... | 2002 | 12209246 |
| apoptosis and apoptosis-related proteins in thyroid myopathies. | dna fragmentation and apoptosis-related proteins have been investigated in thyroid cells and there is evidence that fas-mediated apoptosis is inhibited by thyroid stimulating hormone (tsh). we investigated dna fragmentation by terminal deoxynucleotidyl transferase-mediated dutp nick end labeling (tunel), and bcl-2 and fas antigen expression by immunocytochemistry in skeletal muscles from 12 patients with hypothyroid myopathy and 5 patients with hyperthyroid myopathy. the finding of very few tune ... | 2002 | 12210368 |
| assessment of oropharyngeal cancer. | the records of 87 patients with squamous cell carcinoma of the oropharynx, treated between 1971 and 1998 at kitasato university hospital, were reviewed with the aim of investigating further directions for oropharyngeal cancer treatment. the patients were divided into four major treatment groups: a radiotherapy group; an operation group; a simultaneous chemoradiotherapy group; and a combination treatment group. the 5-year cumulative survival rates for stages i-iv were 75%, 78%, 68% and 41%, respe ... | 2002 | 12212590 |
| cloning and high expression in e.coli of a chimeric gene coding for rice fructose-l,6-bisphosphate aldolase. | a chimeric gene (l 104 bp) coding for rice fructose-l,6-bisphosphate aldolase has been constructed by dna recombination of a synthetic 5'- fragment (-24 to 272) and an rt-pcr amplified product at restriction site s fu i. the synthetic fragment was assembled from six oligonucleotides by t4 dna ligase reaction according to a single-stranded dna method (chen h-b et al, nucleic acids res 1990, 18, 871-878), the pcr amplified fragment (217 - 1 080) was obtained by carrying out a pcr in the presence o ... | 1997 | 12215778 |
| training intensity influences leptin and thyroid hormones in highly trained rowers. | leptin (l) is associated with body-weight-regulating and adipostatic functions. its receptors also may be found centrally. thyroid hormones regulate metabolic processes mainly by binding at peripheral receptors. aim of this study was to show if there is a link between those central and peripheral regulation systems and to investigate the influence of different training intensities on l and the hypothalamic-thyroid-axis (hta) in highly trained rowers. six rowers (18.9 +/- 2.6 y; bmi 22.8 +/- 2.1 ... | 2002 | 12215961 |
| a model binding site for testing scoring functions in molecular docking. | prediction of interaction energies between ligands and their receptors remains a major challenge for structure-based inhibitor discovery. much effort has been devoted to developing scoring schemes that can successfully rank the affinities of a diverse set of possible ligands to a binding site for which the structure is known. to test these scoring functions, well-characterized experimental systems can be very useful. here, mutation-created binding sites in t4 lysozyme were used to investigate ho ... | 2002 | 12217695 |
| detection and characterization of xenon-binding sites in proteins by 129xe nmr spectroscopy. | xenon-binding sites in proteins have led to a number of applications of xenon in biochemical and structural studies. here we further develop the utility of 129xe nmr in characterizing specific xenon-protein interactions. the sensitivity of the 129xe chemical shift to its local environment and the intense signals attainable by optical pumping make xenon a useful nmr reporter of its own interactions with proteins. a method for detecting specific xenon-binding interactions by analysis of 129xe chem ... | 2002 | 12217701 |
| clinical report on the l95p mutation in a dutch family with paraganglioma. | to describe the new l95p mutation of the paraganglioma 1 gene for glomus tumors in a dutch paraganglioma 1 family with six affected family members and to report the clinical findings and results of treatment in nine glomus tumors with a maximum follow-up of 34 years. | 2002 | 12218630 |
| [surgical methods of removal of t4 nasopharyngeal malignancies. a preliminary report on 18 cases]. | to describe the technique and evaluate postoperative sequelae and results of transfacial surgical removal of t4n0 nasopharyngeal malignant neoplasms. | 2002 | 12218867 |
| [plasma t4-concentration and plasma tsh-concentration important parameters for diagnosis of hypothyroidism in dogs]. | 2002 | 12219595 | |
| structure of a trna repair enzyme and molecular biology workhorse: t4 polynucleotide kinase. | t4 phage polynucleotide kinase (pnk) was identified over 35 years ago and has become a staple reagent for molecular biologists. the enzyme displays 5'-hydroxyl kinase, 3'-phosphatase, and 2',3'-cyclic phosphodiesterase activities against a wide range of substrates. these activities modify the ends of nicked trna generated by a bacterial response to infection and facilitate repair by t4 rna ligase. dna repair enzymes that share conserved motifs with pnk have been identified in eukaryotes. pnk con ... | 2002 | 12220496 |
| [neurohumoral effect of xenon]. | clinical trials were carried out in 44 patients during cholecystectomies. the patients were divided into 2 groups (22 pts each) receiving (1) xe:o2 (70:30) or (2) n2o:o2 (70:30) + phentanyl (0.1 ml boluses every 20 min). neurohumoral parameters (acth, hydrocortisone, sth, prolactin, aldosterone, insulin) were almost identically activated in both groups. however, xe monoanesthesia provided an anabolic pattern and better preserved the defense mechanisms of the organism in comparison with n2o + phe ... | 2002 | 12221873 |
| emm typing of group a streptococcus clinical isolates: identification of dominant types for throat and skin isolates. | t and emm types were determined for group a streptococci isolated from patients with various infections during 1990-1999 in toyama prefecture, japan. out of 906 isolates, 872 isolates were divided into 20 t serotypes, and 34 isoltes were t nontypeable (tnt). t12, t1, and t4 were dominant among 699 throat isolates; on the other hand, t11, t28, tb3264, and tnt were dominant among 80 skin isolates. the emm types of 190 isolates were determined following specific pcr amplification and sequencing of ... | 2002 | 12222927 |
| proton concentration (ph) switches the higher-order structure of dna in the presence of spermine. | single-chain observations on the conformational change of giant dna (t4 dna) molecules were performed using fluorescence microscopy at different values of ph in the presence of spermine. individual dna molecules undergo a large discrete change, or all-or-none transition, in conformation from a folded compact state to an unfolded coil state with an increase in ph. this abrupt unfolding of dna with an increase in ph is attributed to a decrease in the concentration of the tetravalent form in spermi ... | 2002 | 12223238 |
| pretibial myxedema as the initial manifestation of graves' disease. | pretibial myxedema (pm) is a localized thickening of the pretibial skin due to accumulation of acid mucopolysacharides (glycosaminoglycans). its pathogenesis is still under investigation. pretibial myxedema, exophthalmus and thyroid acropachy are the dassic extrathyroidal manifestations of graves' disease. almost invariably, pm follows the onset of ophthalmopathy, developing after the diagnosis and treatment of hyperthyroidism. pretibial myxedema preceding graves' ophthalmopathy is rare. we repo ... | 2002 | 12224697 |
| little or no repair of cyclobutyl pyrimidine dimers is observed in the organellar genomes of the young arabidopsis seedling. | a southern-blot-based, site-specific assay for ultraviolet (uv)-induced cyclobutyl pyrimidine dimers (cpds), employing the cpd-specific enzyme t4 endonuclease v, was used to follow the repair of this lesion in particular dna sequences in 5- to 6-d-old arabidopsis thaliana seedlings. cpds, measured as enzyme-sensitive sites, in nuclear sequences were removed rapidly in the light but were repaired slowly, if at all, in the dark. this result was identical to that obtained in prior analyses of cpds ... | 1996 | 12226273 |
| quantitative real-time pcr identifies a critical region of deletion on 22q13 related to prognosis in oral cancer. | quantitative real time pcr was performed on genomic dna from 40 primary oral carcinomas and the normal adjacent tissues. the target genes ecgfb, dia1, bik, and pdgfb and the microsatellite markers d22s274 and d22s277, mapped on 22q13, were selected according to our previous loss of heterozygosity findings in head and neck tumors. quantitative pcr relies on the comparison of the amount of product generated from a target gene and that generated from a disomic reference gene (gapdh-housekeeping gen ... | 2002 | 12226751 |
| association of anion gap with thyroid dysfunction and nodular goiter in capd patients. | to investigate the association of clinical parameters and serum anion gap with thyroid dysfunction and nodular goiter in continuous ambulatory peritoneal dialysis (capd) patients. | 2002 | 12227399 |
| bacteriophage t4 rna ligase 2 (gp24.1) exemplifies a family of rna ligases found in all phylogenetic domains. | rna ligases participate in repair, splicing, and editing pathways that either reseal broken rnas or alter their primary structure. bacteriophage t4 rna ligase (gp63) is the best-studied member of this class of enzymes, which includes yeast trna ligase and trypanosome rna-editing ligases. here, we identified another rna ligase from the bacterial domain--a second rna ligase (rnl2) encoded by phage t4. purified rnl2 (gp24.1) catalyzes intramolecular and intermolecular rna strand joining through lig ... | 2002 | 12228725 |
| surgical management of bladder carcinoma. | despite advances in medical oncology, radiation therapy, and molecular and cell biology, the mainstay in the management of bladder cancer continues to be surgery. | 2002 | 12228754 |
| radiation and hormonal therapy for locally advanced and clinically localized prostate cancer. | patients with locally advanced prostate cancer (tnm clinical stage t3, t4) who were treated using external-beam radiotherapy (ebrt) and 3 years of androgen deprivation therapy (adt) were compared with patients treated with ebrt alone and were shown to have a survival benefit. studies that address the same question for patients with clinically localized disease (stage t1, t2) have been completed and are awaiting follow-up study. a rate of decrease in the serum hemoglobin level of > or =1 g/dl dur ... | 2002 | 12231043 |
| measurement of uvb-induced dna damage and its consequences in models of immunosuppression. | exposure to uvb results in formation of cyclobutane pyrimidine dimers (cpds) and 6-4 photoproducts in dna. these can be quantified by a variety of techniques including alkaline gel electrophoresis, elisas, southwestern blotting, and immunohistochemistry. damage to dna results in activation of damage response pathways, as indicated by western blotting using antibodies specific for p53 and breast cancer-associated gene 1 (brca1) phosphorylation. the signal from dna damage to activation of these re ... | 2002 | 12231188 |
| effect of chronic thyroxine treatment on catalepsy in rats. | the effect of chronic thyroid hormone (thyroxine, t4) administration on the duration of cataleptic freezing was studied in males of random-bred wistar and genetic cataleptic (gc) rat strains. it was found that thyroidectomy brought about a sharp increase in immobility time in wistar rats. replacement with 0.015 mg/kg per day of t4 for 30 days from the day after thyroidectomy prevented the development of predisposition to catalepsy, whereas the same dose of t4 failed to attenuate the predispositi ... | 2002 | 12231448 |
| significance of surgery in the multimodality treatment of rectal cancer. | since the nih recommendations in 1990 the majority of patients with rectal cancer are treated by a multimodality approach. the last decade has seen considerable improvements in the overall management of rectal cancer, therefore a certain reorientation seems justified. although many questions remain to be answered, some general recommendations for the treatment of rectal cancer focussed on the small pelvis can be given. surgery with total mesorectal excision is the standard therapy for cancers of ... | 2002 | 12232483 |
| uniportal endoscopic thoracic sympathectomy for treatment of palmar and axillary hyperhidrosis: analysis of 2000 cases. | primary hyperhidrosis of the upper limbs is a common and troublesome condition in taiwan. therefore, we present our experience in treating hyperhidrosis via uniportal endoscopic thoracic sympathectomy. | 2002 | 12234434 |
| effects of choice of reverse-transcriptase enzyme and use of t4 gene 32 protein on banding patterns in agarose gel differential display. | 2002 | 12234483 | |
| a randomised comparison of bicalutamide ('casodex') 150 mg versus placebo as immediate therapy either alone or as adjuvant to standard care for early non-metastatic prostate cancer. first report from the scandinavian prostatic cancer group study no. 6. | to assess the efficacy and tolerability of bicalutamide 150 mg ('casodex'(1)) as immediate therapy, either alone or as adjuvant to treatment of curative intent, in patients with early (t1b-t4, any n, m0) prostate cancer. | 2002 | 12234503 |
| sequence analysis of bacteriophage t4 dna packaging/terminase genes 16 and 17 reveals a common atpase center in the large subunit of viral terminases. | phage dna packaging is believed to be driven by a rotary device coupled to an atpase 'motor'. recent evidence suggests that the phage dna packaging motor is one of the strongest force-generating molecular motors reported to date. however, the atpase center that is responsible for generating this force is unknown. in order to identify the dna translocating atpase, the sequences of the packaging/terminase genes of coliphages t4 and rb49 and vibriophages kvp40 and kvp20 have been analyzed. alignmen ... | 2002 | 12235385 |
| coupling of a targeting peptide to plasmid dna using a new type of padlock oligonucleotide. | we have recently described a new method for attaching padlock oligonucleotides to supercoiled plasmid dna at specific sequences. a variant of this method has been developed in order to allow the coupling of targeting moieties to plasmids using a convenient strategy. after sequence-specific winding around the double-stranded target dna sequence by ligand-induced triple helix formation, the extremities of a triplex-forming oligonucleotide hybridize to each other, leaving a dangling single-stranded ... | 2002 | 12236796 |
| endocrinology in intensive care medicine: new insights and therapeutic consequences. | sepsis, excessive inflammation, multiple organ failure and weakness prolong the need for intensive care in critically ill patients. furthermore, the risk of death is high in the prolonged critically ill patient (20% after two weeks and 30% after 3 weeks). in prolonged critical illness, protein hypercatabolism and relative preservation of adipose tissue with fatty infiltration of vital organ systems is present. in view of the crucial role of the hypothalamus-pituitary axis for metabolic homeostas ... | 2002 | 12238241 |
| [cis-atracurium--an equivalent substitution for atracurium in pediatric anesthesia?]. | cis-atracurium is a stereoisomer of atracurium, about five times more potent than the racemate. whereas cis-atracurium is routinely used in adults, its effects on children are still poorly defined. we compared equipotent doses of atracurium and cis-atracurium in children aged between 2 and 12 years regarding the quality of neuromuscular blockade, the intubation conditions and the occurrence of side-effects. after approval by the ethics committee and with informed parental consent, 84 children (a ... | 2002 | 12238266 |
| clinical performance and biocompatibility of poly(2-methoxyethylacrylate)-coated extracorporeal circuits. | poly(2-methoxyethylacrylate) is an amphiphilic organic polymer consisting of a hydrophobic backbone with pendant hydrophilic groups that has been reported to reduce protein and platelet adsorption in in vitro and ex vivo studies. | 2002 | 12238845 |
| results of laparoscopic versus open resections for non-early rectal cancer in patients with a minimum follow-up of four years. | laparoscopic rectal resection for malignancy is still debated. concern has been expressed regarding the lack of significant data from larger patient series with longer periods of follow-up. the aims of this study were to compare long-term outcome with a minimum follow-up of four years in unselected patients undergoing either laparoscopic rectal resection or open rectal resection for cancer. | 2002 | 12239902 |
| effects of continuous light and melatonin treatment on energy metabolism of the rat. | melatonin affects food intake, body mass and adiposity of several mammals, but the effects of melatonin on energy metabolism remain largely unknown. this study investigated subacute effects of persistent melatonin treatment and continuous light on carbohydrate and fat metabolism of rat liver and kidney. the male and female rats (no.=40) were maintained either in 12l:12d photoperiod or in constant light. half the rats in both lighting conditions were treated with continuous-release melatonin impl ... | 2002 | 12240904 |
| diffuse sclerosing variant of papillary thyroid carcinoma with primary squamous cell carcinoma. | a case of a very rare combination of diffuse sclerosing variant of papillary thyroid carcinoma (dspc) and primary squamous thyroid carcinoma (psc) is presented. a 25-yr-old woman with right-sided neck mass and hypothyroidism was admitted. us showed that the right lobe of the thyroid gland was enlarged with irregular margins and heterogen echogenity and there were multiple small punctate echogenic foci in the central portion. a scintigraphy with 99mtc showed decreased uptake in the right lobe. fn ... | 2002 | 12240907 |
| olfactory neuroblastomas: survival rate and prognostic factor. | olfactory neuroblastomas are known to be rare and clinically malignant tumors. the authors analyzed the clinical features, long-term treatment outcomes, and prognostic factors related to 21 cases of olfactory neuroblastomas, treated between january 1979 and october 2000, retrospectively reviewed with medical records and radiologic findings. the mean follow-up period was 28.7 months (range 4-178). the extent of tumor was classified according to the ucla staging system. statistical analysis for su ... | 2002 | 12241118 |
| mutational analysis of bacteriophage t4 asia: involvement of n- and c-terminal regions in binding to sigma(70) of escherichia coli in vivo. | the t4 asia is an anti-sigma factor encoded by an early gene of bacteriophage t4. asia has been shown to inhibit t4 early promoters in vitro and expression of this protein from a plasmid causes transcriptional shut off in the host cells leading to cell death. by reasoning that mutant asia expression in escherichia coli will not inhibit the host transcription and hence lead to healthy colony formation, a strategy was developed wherein inactive or partially active mutants of asia could be isolated ... | 2002 | 12242019 |
| comparison of toxicity studies based on the draft protocol for the 'enhanced oecd test guideline no. 407' and the research protocol of 'pubertal development and thyroid function in immature male rats' with 6-n-propyl-2-thiouracil. | two repeated-dose studies of 6-n-propyl-2-thiouracil (ptu) in male rats based on the research protocol 'pubertal development and thyroid function in immature male rats' (pubertal assay) proposed by the endocrine disrupter screening and testing advisory committee (edstac) and the draft protocol of the 'enhanced oecd test guideline 407' (enhanced tg 407) were performed to investigate the suitability of both assays as screening methods for the detection of endocrine-mediated effects and to compare ... | 2002 | 12242606 |
| the role of thyroid hormone on phenylhydrazine hydrochloride mediated inhibitory effects on blood acetylcholinesterase: an in vivo and in vitro study. | a novel phenomenon of protective counteraction by thyroid hormone has been demonstrated in phenylhydrazine hydrochloride (phh) induced insult on blood acetylcholinesterase (ache, ec 3.1.1.7) activity, in both, in vivo and in vitro conditions. injection of phh (20 microg/g) to juvenile male rats for three consecutive days caused a 48% decrease (p < 0.001) in the total blood ache activity on the third day (i.e. 24 h after injections for three consecutive days) in comparison to the control animals. ... | 2002 | 12242684 |
| [tumor angiogenesis--value and significance in squamous epithelial carcinoma of the mouth cavity]. | angiogenesis is an essential condition for the development and proliferation of malignant tumors larger than 3 mm3. furthermore, the development of new blood vessels is necessary for the growth of metastases in the target organ. a relationship between angiogenic factors and stage of disease was shown for different types of malignancies whereas the data available for oral cancer are limited. this prospective study evaluates 51 primary untreated squamous cell carcinoma of the oral cavity. the expr ... | 2002 | 12242930 |
| adjustment of sweep gas flow during cardiopulmonary bypass. | cardiopulmonary bypass (cpb) is one of the major tools of cardiac surgery. however, no clear data are available for the ideal value of sweep gas flow to oxygenator during cpb. the aim of this study was to determine the best value for sweep gas flow during cpb. thirty patients undergoing isolated cabg were randomly and equally allocated into three groups. sweep gas flow to oxygenator was kept at 1.35 l/min/m2 in group 1, 1.60 l/min/m2 in group 2, and 2.0 l/min/m2 in group 3. all patients were ope ... | 2002 | 12243439 |
| impact of t and n substage on survival and disease relapse in adjuvant rectal cancer: a pooled analysis. | to determine the rates of survival and disease control by tnm and mac stage in three randomized north american rectal adjuvant studies. | 2002 | 12243812 |
| radiochemotherapy with short daily infusion of low-dose oxaliplatin, leucovorin, and 5-fu in t3-t4 unresectable rectal cancer: a phase ii iattgi study. | oxaliplatin (oxa)/5-fluorouracil (5-fu) have confirmed their preclinical synergy in advanced colorectal cancer patients. chemoradiotherapy with 5-fu + leucovorin (lv) is considered the standard treatment in unresectable rectal cancer patients. the objective was to evaluate oxa with 5-fu + lv and concurrent radiotherapy in unresectable rectal cancer patients. | 2002 | 12243813 |
| preoperative chemoradiation using oral capecitabine in locally advanced rectal cancer. | capecitabine (xeloda) is a new orally administered fluoropyrimidine carbamate that was rationally designed to exert its effect by tumor-selective activation. we attempted to evaluate the efficacy and toxicity of preoperative chemoradiation using capecitabine in locally advanced rectal cancer. | 2002 | 12243814 |
| thyroid hormones before and after weight loss in obesity. | little is known about changes in thyroid function in obese children. an influence of leptin on thyroid hormone synthesis has been proposed. | 2002 | 12244007 |
| drug interference with laboratory tests: oral contraceptives. | changes in serum protein levels are produced by oral contraceptives. they reflect hepatic synthesis of proteins. changes range from a 40% decrease in orosomucoid to an 180% increase in ceruloplasmin. alterations in the concentration of some globulin proteins lead to altered serum levels of some hormones and trace metals. plasma levels of cortisol and thyroxine are most affected. an estrogen-induced increase in thyroxine-binding globulin causes a transient reduction in free thyroxine (t4). ... | 1977 | 12260155 |
| thyroid function tests under the effect of steroidal contraceptives compared to normal pregnancy. | the effects of the oral contraceptive pill on th blood and urinary levels of various hormones was studies using 5 groups of women. group 1 consisted of 59 women using combined contraceptive pills for 1 to 12 years under the commercial names of primovlar (25 cases) anovlar (19 cases) and ovral (15) cases; group 2 consisted of 50 pregnant women during the 1st trimester; group 3 consisted of 50 pregnant women during th 2nd trimester; group 4 consisted of 50 pregnant women during the 3rd trimester ... | 1982 | 12266214 |
| remission of hyperthyroidims and oral contraceptive therapy. [answer to question]. | there is no endocrine contraindication to the use of oral contraceptives (ocs) in a 31-year-old nulliparous woman who presumably is in remission from hyperthyroidism. yet, several important physiological and pathophysiological alterations must be appreciated to evaluate properly thyroid function in persons taking estrogen-containing preparations. estrogens induce increased hepatic synthesis and release of thyroxine binding globulins (tbgs). increased serum tbg concentrations result in a new t ... | 1984 | 12266542 |
| circulating thyroid hormone levels in recycled turkey breeder hens during a short day prelighting period and renewal of photosensitivity for egg production. | two experiments were conducted with yearling turkey hens at the end of their first season of egg laying. the purpose was to examine changes in plasma thyroid hormone levels during recycling and renewal of photosensitivity for egg production. plasma concentrations of thyroid hormones were determined weekly or biweekly for 8 wk following a change from existing photoperiods of long days (ld) to short days (sd) and during the associated complete renewal (recycling) of photosensitivity for egg produc ... | 2002 | 12269614 |
| use of adjuvant, preoperative chemo-radiotherapy in patients with locally advanced rectal cancer. | the objective of this study was to perform a non-randomised prospective examination of the efficacy of adjuvant, preoperative chemo-radiotherapy in patients with locally advanced rectal cancer. | 2002 | 12269914 |
| contraception and hiv / std infections. | copper-releasing iuds cause severe dermatitis (e.g., urticarial eruptions and eczema) in copper-sensitive women. such exposure of subcutaneous areas is a cofactor for hiv infection. the excessive menstrual bleeding that accompanies iud use and absorption of virions and infected cells in the semen of hiv-infected males by iud tails facilitates hiv infection. iud tails have the highest concentration of hiv in infected women. hiv causes cervicitis in hiv-infected women. the cross reactivity between ... | 1994 | 12287844 |
| thyrotropin screening for congenital hypothyroidism in queen sirikit national institute of child health, thailand (during year 1995-2000). | to detect newborns with congenital hypothyroidism (ch) by tsh screening and to treat the affected infants as early as possible. to find the incidence of ch in neonates born at rajavithi hospital from 1995 to 2000. the effects of thyroid pathology, ages at the initiation of treatment, associated conditions, and severity of ch on the intellectual outcome of the patients were also analyzed. | 2002 | 12296410 |
| hiv vaccine development and africa. | the genetic variation of hiv poses a great problem in the development of a vaccine that will work against the viral subtypes that predominate in africa. hiv-1 exists in as many as 10 subtypes and these subtypes have 20-30% inter-subtype variation, while differences within a subtype can differ up to 15%. moreover, hiv differs from person to person; it creates so many different versions of itself, which overwhelms the person's immune system. however, many areas of the code are conserved or shar ... | 2000 | 12322293 |
| effects of short-term trimethoprim-sulfamethoxazole administration on thyroid function in dogs. | to determine how rapidly trimethoprim-sulfamethoxazole affects serum total thyroxine (t4) and thyroid-stimulating hormone (tsh) concentrations in euthyroid dogs and how quickly hormone concentrations return to reference values following discontinuation of administration. | 2002 | 12322917 |
| thyroid function in lactating women using the progestasert system. | this study determines the thyroid hormone levels, in serum and milk, of 46 healthy lactating women fitted with the progestasert system immediately postpartum. the ages of the women ranged from 28 to 35 years, parity from 3 to 6. they were divided into 2 groups: 1) menstruating and lactating women, and 2) amenorrheic and lactating mothers. the 1st group was subdivided into: 1) non-iud users (n=11); 2) cu t 200 users (n=9); and 3) progestasert users (n=6). the 2nd group was subdivided into: 1) ... | 1981 | 12336866 |
| [aids: the children also]. | it is no longer possible for health officials or politicians in africa to deny the existence of acquired immune deficiency syndrome (aids) in their countries in the name of nationalism. educational and research programs and appeals for international aid to fight the epidemic have become the rule. almost all sub-saharan african nations have sought world health organization (who) assistance for aids control. children contracting the virus transplacentally before birth are among the population g ... | 1987 | 12341552 |
| [aids: "we will win"]. | an international colloquium on aids held near paris from october 26-28, 1989, unlike the world conference on aids in montreal the year before, was able to find reasons for optimism. significant progress was reported in immunotherapy and in chemotherapy. successful experiments in vaccinating monkeys against the aids virus were reported from the us, france, and zaire. time is needed to prove the efficacy of the vaccines because of the slow development in aids. a vaccine is being tested by jona ... | 1989 | 12342689 |
| effects of oral zinc and magnesium supplementation on serum thyroid hormone and lipid levels in experimentally induced diabetic rats. | this study was designed to investigate the effects of oral zinc and magnesium supplementation on serum thyroid hormone and lipid levels in alloxan-induced diabetic rats. thirty-two albino male rats, weighing 234 +/- 34 g, were divided into four experimental groups (control, diabetic, diabetic+zinc supplemented and diabetic+ magnesium supplemented). the experiment lasted for 60 d. the first 45 d of the experiment was the supplementation and last 15 d was the supplementation and diabetes-inducing ... | 2002 | 12350134 |
| stereological analysis reveals striking differences in the structural plasticity of two readily identifiable glomeruli in the antennal lobes of the adult worker honeybee. | the primary antennal sensory centers (antennal lobes) in the brain of the honeybee are highly compartmentalized into discrete spheres of synaptic neuropil called glomeruli, many of which can be identified according to their predictable size and location. glomeruli undergo significant changes in volume during the lifetime of the adult worker bee, at least some of which are activity dependent. this study tests the commonly expressed assumption that increases in neuropil volume are accompanied by a ... | 2002 | 12351725 |
| preoperative staging of gastric cancer by endoscopic ultrasound: the prognostic usefulness of ascites detected by endoscopic ultrasound. | endoscopic ultrasound (eus) is the standard modality in local preoperative staging of gastric cancers and is reputedly able to detect ascites. however, the association between ascites detected by eus and local tumor staging, peritoneal carcinomatosis, or survival after surgery is not well documented. | 2002 | 12352295 |
| intracellular localization and transcriptional regulation of tumor necrosis factor (tnf) receptor-associated factor 4 (traf4). | to gain insight in the subcellular localization of tumor necrosis factor receptor-associated factor (traf4) we analyzed gfp chimeras of full-length traf4 and various deletion mutants derived thereof. while traf4-gfp (t4-gfp) was clearly localized in the cytoplasm, the n-terminal deletion mutant, t4(259-470), comprising the traf domain of the molecule, and a c-terminal deletion mutant consisting mainly of the ring and zinc finger domains of traf4 were both localized predominantly to the nucleus. ... | 2002 | 12354113 |
| postpartum recurrence of graves' hyperthyroidism can be prevented by the continuation of antithyroid drugs during pregnancy. | previous studies recommend the discontinuation of antithyroid drug (atd) therapy during pregnancy in women with well-controlled graves' hyperthyroidism (gh). in this study, we investigated whether this termination of atd therapy during pregnancy is beneficial in terms of postpartum gh recurrence. | 2002 | 12354128 |
| iodized salt improves the effectiveness of l-thyroxine therapy after surgery for nontoxic goitre: a prospective and randomized study. | to investigate whether the addition of iodized salt to daily diet in thyroidectomized patients for nontoxic goitre could influence the effectiveness of nonsuppressive l-thyroxine (l-t4) therapy on thyroid remnant size, during 12 months' follow-up after thyroid surgery. | 2002 | 12354133 |
| serum concentrations of tumour necrosis factor-alpha (tnf-alpha) and soluble tnf-alpha receptor p55 in patients with hypothyroidism and hyperthyroidism before and after normalization of thyroid function. | tumour necrosis factor-alpha (tnf-alpha) is a cytokine with numerous immunological and metabolic activities. receptors for tnf-alpha have been demonstrated in thyroid follicular cells and tnf-alpha and its receptors have been implicated in the cytotoxic mechanisms that characterize the thyroid destruction in autoimmune thyroid disease. in patients with graves' disease, serum levels of tnf-alpha have been reported to be elevated and administration of tnf-alpha to humans has been shown to induce h ... | 2002 | 12354134 |
| preparation for radioactive iodine administration in differentiated thyroid cancer patients. | because in recent years the practice of tsh suppression has changed, and thyroxine doses have been reduced significantly in the treatment of patients with low-risk differentiated thyroid cancer, the goal of this study was to determine the time needed to attain a target tsh level (of 30 miu/l) following levothyroxine withdrawal in patients treated with thyroxine according to current guidelines, in anticipation of radioactive iodine (rai) administration. | 2002 | 12354135 |
| dynamics of the plasma concentrations of tsh, ft4 and t3 following thyroxine supplementation in congenital hypothyroidism. | the dynamics of the plasma concentrations of various diagnostic determinants of thyroid function were analysed in children with congenital hypothyroidism (ch) after the start of t4 supplementation. the description of the biochemical dynamics of tsh and free t4 (ft4) during the first period of thyroxine treatment is important to depict the practical outlines of the initial dosage of t4 and dosage adjustments for newborns with variable forms of ch. | 2002 | 12354136 |
| surgical treatment for locally advanced (t4) squamous cell carcinoma of the thoracic esophagus. | it is still difficult to decide on the treatment modalities for advanced esophageal carcinoma when the prognostic factors of t4 esophageal cancer are not fully understood. in this article, we report that among 71 patients with t4 thoracic esophageal cancer, 49 underwent esophagectomy, 9 had curative resection (r0 group), and 40 had palliative resection (r1/2 group). a total of 22 patients had palliative treatments: bypass in 5 (bypass group), gastrostomy or jejunostomy in 6 (stoma group), and ra ... | 2002 | 12355140 |
| polyamine levels of human colorectal adenocarcinomas are correlated with tumor stage and grade. | cellular proliferation and differentiation are regulated by polyamines and their rate-limiting enzyme ornithine decarboxylase (odc), both of which are correlated with tumor growth, but their role in differentiation is less clear. we investigated the correlation of odc activity and polyamine levels with tumor stage and grade with respect to sample recruitment. | 2002 | 12355213 |
| serum cortisol and thyroid hormone levels in neonates with sepsis. | to evaluate the thyroid hormone and cortisol levels in neonates with sepsis in relation to the final outcome. it was hypothesized that the hormonal level could act as some prognostic guideline. | 2002 | 12356216 |
| effects of rapeseed-press cake glucosinolates and iodine on the performance, the thyroid gland and the liver vitamin a status of pigs. | rapeseed press cake (per kg dm 181 g ee, 341 g cp and 23.3 mmol glucosinolates) was tested in a long-term experiment with a total of sixty pigs (live weight range 24 to 104 kg). the 3 x 2 factorial design consisted of three rapeseed press cake levels (no rapeseed press cake--control, 75 g or 150 g rapeseed press cake per kg diet) each with two iodine dosages (125 or 250 micrograms supplementary iodine per kg diet). reduced feed intake and depressed weight gain were found in groups receiving 150 ... | 2001 | 12357593 |
| phenotypic reversion or death of cancer cells by altering signaling pathways in three-dimensional contexts. | we previously used a three-dimensional (3d) reconstituted basement membrane (rbm) assay to demonstrate that tumorigenic hmt-3522 t4-2 human breast cells can be induced to form morphologically normal structures ("reversion") by treatment with inhibitors of beta1 integrin, the epidermal growth factor receptor (egfr), or mitogen-activated protein kinase (mapk). we have now used this assay to identify reversion and/or death requirements of several more aggressive human breast cancer cell lines. | 2002 | 12359858 |
| [adjuvant and neoadjuvant radiochemotherapy of rectal carcinoma]. | adequate surgical treatment is a prerequisite for any adjuvant and neoadjuvant therapy of rectal cancer. randomized studies have investigated the role of radiotherapy for more than 20 years. at least two conclusions can be drawn from the data available by now: 1. radiotherapy combined with 5-fu-chemotherapy is more effective than radiotherapy alone in the adjuvant setting. this prompted a national cancer institute consensus conference in the usa in 1990 and a german cancer society consensus conf ... | 2002 | 12360683 |
| palpable thyroid and parathyroid nodules in asymptomatic cats. | client-owned cats underwent surgery to remove palpable cervical masses in cats with normal total t4 values and no clinical signs of hyperthyroidism, renal disease, or hyperparathyroidism. non-functional thyroid and parathyroid adenomas were found and identified by histopathological examination. the significance of these findings is discussed in relation to palpating a goiterous mass in an asymptomatic cat. | 2002 | 12360953 |
| after cystectomy, is it justified to perform a bladder replacement for patients with lymph node positive bladder cancer? | after cystectomy for bladder cancer, when pelvic lymph nodes are positive, bladder replacement remains controversial. the aim of this study was to evaluate the outcome of patients who underwent neobladder replacement despite bladder cancer metastasis to the regional lymph nodes. | 2002 | 12361899 |
| predicting the structure of protein cavities created by mutation. | to assist in the efficient design of protein cavities, we have developed a minimization strategy that can predict with accuracy the fate of cavities created by mutation. we first modelled, under different conditions, the structures of six t4 lysozyme and cytochrome c peroxidase mutants of known crystal structure (where long, hydrophobic, buried side chains have been replaced by shorter ones) by minimizing the virtual structures derived from the corresponding wild-type co-ordinates. an unconstrai ... | 2002 | 12364581 |