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survival and transfer efficacy of mixed strain salmonella enterica ser. typhimurium from beef burgers to abiotic surfaces and determination of individual strain contribution.the aim of the study was to evaluate the survival and transfer efficacy of 3 salmonella typhimurium strains from beef burgers to abiotic surfaces and determine the individual strain distribution. s. typhimurium population on beef burgers during incubation remained constant at initial levels of contamination approximately 3 and 5 log cfu/g. additionally, the survival of pathogens on soiled hdpe surfaces was significant during incubation at both initial inocula, while ca 1.5 log cfu/cm(2) reductio ...201728431296
effect of salmonella enterica serovar typhimurium vnp20009 and vnp20009 with restored chemotaxis on 4t1 mouse mammary carcinoma progression.a variety of bacterial strains have been evaluated as bio-therapeutic and immunomodulatory agents to treat cancer. one such strain, salmonella enterica serovar typhimurium vnp20009, which is attenuated by a purine auxotrophic mutation and modified lipid a, is characterized in previous models as a safely administered, tumor colonizing agent. however, earlier work tended to use less aggressive cancer cell lines and immunocompromised animal models. here, we investigated the safety and efficacy of v ...201728431394
sensitivity to antibiotics of bacteria exposed to gamma radiation emitted from hot soils of the high background radiation areas of ramsar, northern iran.over the past several years our laboratories have investigated different aspects of the challenging issue of the alterations in bacterial susceptibility to antibiotics induced by physical stresses.201728432369
electrostatic spraying of organic acids on biofilms formed by e. coli o157:h7 and salmonella typhimurium on fresh produce.electrostatic spraying which has an even and retained surface coverage could be an effective novel technique to completely cover the surface of fresh produce to disrupt biofilm formation by pathogenic bacteria. spinach leaves and cantaloupe rind were spot-inoculated with a bacterial culture and stored at 8°c for 72h to allow biofilm formation. among various green fluorescent protein-labeled strains, ed 14 strain of e. coli o157:h7 and sd 10strain of salmonella typhimurium had the best attachment ...201528433292
eggshell penetration by salmonella typhimurium in table eggs: examination of underlying eggshell structures by micro-computed tomography and scanning electron microscopy.horizontal infection of table eggs by food borne, human infection causative agents such as salmonella is a serious concern for consumers and industry. in this study, we investigated the relationship between eggshell translucency, mammillary layer abnormalities and pore structure using computed tomography (ct) and scanning electron microscopy. the effects of eggshell pore structure, size and number on salmonella typhimurium penetration was also investigated. the eggs were infected with s. typhimu ...201528433301
optimizing the restored chemotactic behavior of anticancer agent salmonella enterica serovar typhimurium vnp20009.bacteria, including strains of salmonella, have been researched and applied as therapeutic cancer agents for centuries. salmonella are particularly of interest due to their facultative anaerobic nature, facilitating colonization of differentially oxygenated tumor regions. additionally, salmonella can be manipulated with relative ease, resulting in the ability to attenuate the pathogen or engineer vectors for drug delivery. it was recently discovered that the anti-cancer salmonella enterica serov ...201728433721
multidrug resistance salmonella genomic island 1 in a morganella morganii subsp. morganii human clinical isolate from france.salmonella genomic island 1 (sgi1) is a multidrug resistance integrative mobilizable element that harbors a great diversity of antimicrobial resistance gene clusters described in numerous salmonella enterica serovars and also in proteus mirabilis. a serious threat to public health was revealed in the recent description in p. mirabilis of a sgi1-derivative multidrug resistance island named pgi1 (proteus genomic island 1) carrying extended-spectrum-β-lactamase (esbl) and metallo-β-lactamase resist ...201728435889
qsar modeling for predicting mutagenic toxicity of diverse chemicals for regulatory purposes.the safety assessment process of chemicals requires information on their mutagenic potential. the experimental determination of mutagenicity of a large number of chemicals is tedious and time and cost intensive, thus compelling for alternative methods. we have established local and global qsar models for discriminating low and high mutagenic compounds and predicting their mutagenic activity in a quantitative manner in salmonella typhimurium (ta) bacterial strains (ta98 and ta100). the decision t ...201728435990
immune response to salmonella infections in vaccinated and non-vaccinated turkeys.vaccination has been widely used to reduce the salmonella burden in poultry and subsequently the transmission to humans. concerning turkey, there is little knowledge on the immune response to colonization and invasion by salmonella species or about efficacy of vaccination and involved immune mechanisms. in the present study, turkeys were vaccinated at the day of hatch and infected with salmonella typhimurium (st) or enteritidis (se) field strains three weeks later. a control group was kept uninf ...201728437624
invs coordinates expression of prgh and fimz and is required for invasion of epithelial cells by salmonella enterica serovar typhimurium.deep sequencing has revolutionized our understanding of the bacterial rna world and has facilitated the identification of 280 small rnas (srnas) in salmonella despite the suspicions that srnas may play important roles in salmonella pathogenesis, the functions of most srnas remain unknown. to advance our understanding of rna biology in salmonella virulence, we searched for srnas required for bacterial invasion into nonphagocytic cells. after screening 75 srnas, we discovered that the ablation of ...201728439039
evaluation of the probiotic strain bifidobacterium longum subsp. infantis cect 7210 capacities to improve health status and fight digestive pathogens in a piglet model.probiotics have been demonstrated to be useful to enhance gut health and prevent gastrointestinal infections. the objective of this study is to demonstrate the potential of the probiotic strain bifidobacterium longum subsp. infantis cect 7210 (b. infantis im1) to prevent and fight intestinal disease by using a salmonella typhimurium (trial 1) or an enterotoxigenic escherichia coli k88 (trial 2) oral challenge in a weaning piglet model. seventy-two piglets were used in each trial. after an adapta ...201728443068
genetic and dietary iron overload differentially affect the course of salmonella typhimurium infection.genetic and dietary forms of iron overload have distinctive clinical and pathophysiological features. hfe-associated hereditary hemochromatosis is characterized by overwhelming intestinal iron absorption, parenchymal iron deposition, and macrophage iron depletion. in contrast, excessive dietary iron intake results in iron deposition in macrophages. however, the functional consequences of genetic and dietary iron overload for the control of microbes are incompletely understood. using hfe(+/+) and ...201728443246
the legacy of genetic analysis advances contemporary research with escherichia coli k-12 and salmonella enterica serovar typhimurium lt2.escherichia coli k-12 and salmonella enterica serovar typhimurium lt2 became standard organisms for genetic analysis during the truman administration. half a century later, genetic analysis with these strains had become an art form, interpreted through 23 articles in the ambitious two-volume masterpiece edited by the late fred neidhardt and colleagues. these legacy articles now are available through ecosal plus, so as to inform and inspire contemporary genetic analyses in these standard organism ...201728443537
draft genome sequences of salmonella enterica subsp. enterica serovar typhimurium strains isolated from chicken and swine carcasses in two distinct geographical regions from rio de janeiro state, brazil.salmonella enterica subsp. enterica serovar typhimurium is a surveyed worldwide serotype with well-characterized genomes for several different strains. in brazil, very few studies have submitted whole-genome sequences to genbank. this genome may be useful to analyze the genetic mechanisms comparable to those of other related studies conducted in brazil and globally.201728450502
molecular characterization of silver-stearate nanoparticles (agstnps): a hydrophobic and antimicrobial material against foodborne pathogens.in this study, silver-stearate nanoparticles (agstnps) have been produced from silver nitrate solutions by replacing the nitrate by stearate ions and then reducing by thermal treatment. agstnps were characterized by particle size, zeta-potential, atr-ftir, contact angle and sem (scanning electron microscopy) analyses. the mean particle size and zeta potential of agstnps were determined to be 69.22±7.30nm and +8.02±0.88mv. atr-ftir analysis showed characteristic ir bands of stearate, revealing th ...201528455024
isolation and characterization of salmonella enteritidis and salmonella typhimurium from chicken meat in egypt.salmonella enterica serovars enteritidis and typhimurium represent the major serovars associated with human salmonellosis. contamination of meat products with these serovars is considered the main source of infection.201728459222
the transfer rate of salmonella typhimurium from contaminated parsley to other consecutively chopped batches via cutting boards under different food handling scenarios.in many mediterranean and middle eastern countries, leafy green parsley is typically eaten raw and prepared by fine chopping several batches. this study aimed to quantify the transfer rate of s. typhimurium (tr) across all chopped batches in scenarios that resemble normally occurring operations in restaurants and home kitchens. fresh parsley leaves were inoculated at concentrations of 6 and 3logcfu/g and chopped on a polyethylene cutting board (cb). uninoculated parsleys were sequentially choppe ...201628460944
quantification of transfer of salmonella from citrus fruits to peel, edible portion, and gloved hands during hand peeling.although studies have quantified bacterial transfer between hands and various materials, cross-contamination between the surface of fresh citrus fruit and the edible portions during hand peeling has not been reported. this study quantifies transfer of salmonella to the edible portion of citrus fruit from a contaminated peel during hand peeling. citrus fruits used for this study were citrus sinensis (sweet orange) cultivars 'valencia' and 'navel', citrus unshiu (satsuma mandarins), citrus reticul ...201728463082
2-nitroanisole-induced oxidative dna damage in salmonella typhimurium and in rat urinary bladder cells.2-nitroanisole (2-na) is used in the manufacturing of azo dyes and causes cancer, mainly in the urinary bladder. previous in vivo genotoxic data seems to be insufficient to explain the mechanism through which 2-na induces carcinogenesis, and several bladder carcinogens were reported to induce oxidative dna damage. thus, we examined the potential induction of oxidative dna damage by 2-na using bacterial strain yg3008, a mutmst-deficient derivative of strain ta100. consequently, strain yg3008, whe ...201728464992
salmonella typhimurium infection reduces schistosoma japonicum worm burden in mice.coinfection of microorganisms is a common phenomenon in humans and animals. in order to further our understanding of the progress of coinfection and the possible interaction between different pathogens, we have built a coinfection mouse model with schistosoma japonicum and salmonella typhimurium, and used this model to investigate the systemic metabolic and immune responses using nmr-based metabonomics and immunological techniques. our results show that salmonella typhimurium (atcc14028) infecti ...201728465515
subtractive protein profiling of salmonella typhimurium biofilm treated with dmso.salmonella typhimurium is an important biofilm-forming bacteria. it is known to be resistant to a wide range of antimicrobials. the present study was carried out to evaluate the effects of dimethyl sulfoxide (dmso) against s. typhimurium biofilm and investigate whole-cell protein expression by biofilm cells following treatment with dmso. antibiofilm activities were assessed using pellicle assay, crystal violet assay, colony-forming unit counting and extracellular polymeric substance (eps) matrix ...201728470375
anti-tumor activity of an immunotoxin (tgfα-pe38) delivered by attenuated salmonella typhimurium.the anticancer strategy underlying the use of immunotoxins is as follows: the cancer-binding domain delivers the toxin to a cancer cell, after which the toxin enters and kills the cell. tgfα-pe38 is an immunotoxin comprising transforming growth factor alpha (tgfα), a natural ligand of epidermal growth factor receptor (egfr), and a modified pseudomonas exotoxin a (pe38) lacking n terminal cell-binding domain, a highly potent cytotoxic protein moiety. tumor cells with high level of egfr undergo ap ...201728473665
modeling of salmonella typhimurium growth under the effects of carum copticum essential oil, temperature, ph and inoculum size.the purpose of this study was to elucidate some factors affecting the growth of salmonella typhimurium. these factors included carum copticum essential oil (0%, 0.015%, 0.03% and 0.06%), temperature (25 ˚c and 35 ˚c), ph (5, 6 and 7) and inoculum size (10(3) and 10(5) cfu ml(-1)). brain heart infusion broth was used as the growth medium. there were 48 treatment combinations and the experiment was carried out in triplicate. growth was monitored by visible turbidity over a 30 days period. a parame ...201728473899
assessment of consumer exposure to salmonella spp., campylobacter spp., and shiga toxin-producing escherichia coli in meat products at retail in the city of sao paulo, brazil.meat products may be vehicles of bacterial pathogens to humans, and salmonella spp., campylobacter spp., and shiga toxin-producing escherichia coli (stec) are the most relevant. the aim of this study was to generate data on prevalence of these three pathogens in 552 samples of meat products (hot dogs, pork sausages, raw ground beef, and raw chicken legs) sold at retail in the city of sao paulo, brazil. salmonella spp. was detected in 5.8% (32/552) of samples, comprising pork sausages 62.5% (20/3 ...201728475359
efficacy of nh3 as a secondary barrier treatment for inactivation of salmonella typhimurium and methicillin-resistant staphylococcus aureus in digestate of animal carcasses: proof-of-concept.managing the disposal of infectious animal carcasses from routine and catastrophic disease outbreaks is a global concern. recent research suggests that burial in lined and aerated trenches provides the rapid pathogen containment provided by burial, while reducing air and water pollution potential and the length of time that land is taken out of agricultural production. survival of pathogens in the digestate remains a concern, however. a potential answer is a 'dual'-barrier approach in which ammo ...201728475586
emergence of multidrug resistance in locally-acquired human infections with salmonella typhimurium in australia owing to a new clade harbouring blactx-m-9.antimicrobial resistance in non-typhoidal salmonella is a critical problem globally, with the emergence of resistance to third-generation cephalosporins (3gcs) a particular concern. the aim of this study was to use whole-genome sequencing (wgs) to characterise recently identified human and non-human isolates of 3gc-resistant salmonella enterica subsp. enterica serovar typhimurium from australia. the illumina nextseq sequencing platform was used to determine the genome sequences of 78 s. typhimur ...201728476613
holocranohistochemistry enables the visualization of α-synuclein expression in the murine olfactory system and discovery of its systemic anti-microbial effects.braak and del tredici have proposed that typical parkinson disease (pd) has its origins in the olfactory bulb and gastrointestinal tract. however, the role of the olfactory system has insufficiently been explored in the pathogeneses of pd and alzheimer disease (ad) in laboratory models. here, we demonstrate applications of a new method to process mouse heads for microscopy by sectioning, mounting, and staining whole skulls ('holocranohistochemistry'). this technique permits the visualization of ...201728477284
microbial compositional changes in broiler chicken cecal contents from birds challenged with different salmonella vaccine candidate strains.previously, we constructed and characterized the vaccine efficacy of salmonella typhimurium mutant strains in poultry with either inducible mvin expression (pbad-mvin) or methionine auxotrophy (δδmetrmetd). the aim of the present study was to assess potential impact of these salmonella vaccine strains on the cecal microbiota using a next generation sequencing (ngs). the cecal microbial community obtained from unvaccinated (group 1) and vaccinated chickens (group 2, vaccinated with pbad-mvin; gro ...201728479173
evaluation of anticancer potential of eight vegetal species from the state of oaxaca.eight plant species from oaxaca, some of them used in traditional medicine, were subjected to screening of several biological activities to provide data regarding their anticancer potential, although no scientific information is available about their pharmacological effects.201728480417
lubac-synthesized linear ubiquitin chains restrict cytosol-invading bacteria by activating autophagy and nf-κb.cell-autonomous immunity relies on the ubiquitin coat surrounding cytosol-invading bacteria functioning as an 'eat-me' signal for xenophagy. the origin, composition and precise mode of action of the ubiquitin coat remain incompletely understood. here, by studying salmonella typhimurium, we show that the e3 ligase lubac generates linear (m1-linked) polyubiquitin patches in the ubiquitin coat, which serve as antibacterial and pro-inflammatory signalling platforms. lubac is recruited via its subuni ...201728481331
linear ubiquitination of cytosolic salmonella typhimurium activates nf-κb and restricts bacterial proliferation.ubiquitination of invading salmonella typhimurium triggers autophagy of cytosolic bacteria and restricts their spread in epithelial cells. ubiquitin (ub) chains recruit autophagy receptors such as p62/sqstm1, ndp52/calcoco and optineurin (optn), which initiate the formation of double-membrane autophagosomal structures and lysosomal destruction in a process known as xenophagy. besides this, the functional consequences and mechanistic regulation of differentially linked ub chains at the host-salmo ...201728481361
genotypic and phenotypic characterization of multidrug resistant salmonella typhimurium and salmonella kentucky strains recovered from chicken carcasses.salmonella typhimurium is the leading cause of human non-typhoidal gastroenteritis in the us. s. kentucky is one the most commonly recovered serovars from commercially processed poultry carcasses. this study compared the genotypic and phenotypic properties of two salmonella enterica strains typhimurium (st221_31b) and kentucky (sk222_32b) recovered from commercially processed chicken carcasses using whole genome sequencing, phenotype characterizations and an intracellular killing assay. illumina ...201728481935
characterization of clinically isolated antibiotic-resistant salmonella typhimurium exposed to subinhibitory concentrations of ceftriaxone and ciprofloxacin.this study was designed mainly to assess the phenotypic properties of clinically isolated salmonella typhimurium exposed to ceftriaxone and ciprofloxacin. the antibiotic susceptibility, β-lactamase activity, efflux activity, bacterial motility, biofilm-forming ability, and gene expression were determined in s. typhimurium atcc 19585 and s. typhimurium ccarm 8009 when exposed to subinhibitory concentrations of ceftriaxone and ciprofloxacin. s. typhimurium ccarm 8009 was highly resistant to ampici ...201728486078
sodium bisulfate and a sodium bisulfate/tannin mixture decreases ph when added to an in vitro incubated poultry cecal or fecal contents while reducing salmonella typhimurium marker strain survival and altering the microbiome.the objective of the present study was to investigate the ability of animal feed-grade sodium bisulfate (sbs) and a mixture of sodium bisulfate/tannin to inhibit the growth of salmonella using an anerobic in vitro mixed cecal culture to mimic the conditions within the chicken cecum. an initial inoculum of salmonella typhimurium was introduced to an anerobic dilution solution containing 1/3000 diluted cecal bacteria and solids consisting of ground chicken feed and different percentages of solid s ...201728489978
4,4'-diaponeurosporene-producing bacillus subtilis increased mouse resistance against salmonella typhimurium infection in a cd36-dependent manner.deficient mucosal innate immunity is a hallmark of infectious diarrhea, such as salmonella typhimurium (s. typhimurium)-induced gastroenteritis. here, we report that oral administration of a 4,4'-diaponeurosporene-producing bacillus subtilis (b.s-dia) could improve mice mucosal immunity, as showed by an increased resistance against s. typhimurium infection. intragastric administration of b.s-dia for 7 days could increase the secretion of ccl20 by intestinal epithelial cells (iecs) and then recru ...201728491061
intra-arterial administration of tumor-targeting salmonella typhimurium a1-r regresses a cisplatin-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (pdox) mouse model.previously, a patient-derived orthotopic xenograft (pdox) model was established with a lung metastasis from an osteosarcoma patient which developed after adjuvant cisplatinum (cddp) treatment. in this model, we previously demonstrated the efficacy of trabectedin (trab) and temozolomide (tem) compared with cddp. in the present report, osteosarcoma tissue was implanted orthotopically in the distal femur of mice which were randomized into the following groups when tumor volume reached approximately ...201728494180
anco3, a new member of the emerging family of phage-like plasmids.a phage-like plasmid isolated from a clinical isolate of salmonella enterica serovar derby has strong nucleotide sequence identity to the phage-like plasmids pstm_phi isolated from salmonella enterica serovar typhimurium l495, anco1 and anco2 from escherichia coli 243 and escherichia coli 244, and the virulent salmonella-specific ssu5 bacteriophage.201728495760
implication of sialidases in salmonella infection: genome release of sialidase knockout strains from salmonella enterica serovar typhimurium lt2.sialidases, which are widely distributed in nature, cleave the α-ketosidic bond of terminal sialic acid residue. these emerging virulence factors degrade the host glycan. we report here the release of seven sialidase and one sialic acid transporter deletion in salmonella enterica serovar typhimurium strain lt2, which are important in cellular invasion during infection.201728495784
microwave ablation combined with attenuated salmonella typhimurium for treating hepatocellular carcinoma in a rat model.we aim to investigate the safety and efficacy of microwave ablation (mwa) combined with attenuated salmonella typhimurium strain vnp20009 in treating hepatocellular carcinoma. portions of tumor tissues were orthotopically implanted in the livers of 40 male rats weighed 150~200 g to establish tumor models. three weeks later, the rats were randomly divided into four groups: (a) mwa plus vnp20009 group; (b) mwa group; (c) vnp20009 group; and (d) control group. incomplete mwa was performed (20~30 w, ...201728498813
large-scale recoding of a bacterial genome by iterative recombineering of synthetic dna.the ability to rewrite large stretches of genomic dna enables the creation of new organisms with customized functions. however, few methods currently exist for accumulating such widespread genomic changes in a single organism. in this study, we demonstrate a rapid approach for rewriting bacterial genomes with modified synthetic dna. we recode 200 kb of the salmonella typhimurium lt2 genome through a process we term sircas (stepwise integration of rolling circle amplified segments), towards const ...201728499033
persistence of salmonella typhimurium lt2 in soil enhanced after growth in lettuce medium.the persistence of salmonella in the environment is influenced by a multitude of biotic and abiotic factors. in addition, its persistence can be influenced by preadaptation before the introduction into the environment. in order to study how preadaptation changes the survival of salmonella in soil and therefore its potential to colonize the phytosphere, we developed a new medium based on lettuce material [lettuce medium (lm)]. salmonella enterica serovar typhimurium strain lt2 was used as a model ...201728503171
prebiotic efficacy and mechanism of inulin combined with inulin-degrading lactobacillus paracasei i321 in competition with salmonella.inulin efficacy was proven for raising anti-salmonella activity of the inulin-degrading lactobacillus paracasei i321 (infant isolate) through the mechanisms of antibacterial secretion and competitive adhesion. inulin significantly promoted competitive adhesion of l. paracasei i321 against many gram-negative foodborne pathogenic bacteria. salmonella typhimurium sa2093 was completely suppressed under highly competitive niche of fecal microflora when inulin, l. paracasei i321, and their synbiotic c ...201728504142
synergistic antimutagenic effect of isothiocyanates against varied mutagens.although plant foods provide an array of nutrients in the human diet, our knowledge of how these nutrients are interacting among each other at molecular level is limited. among these, glucosinolates are the most important secondary metabolites, which are readily hydrolyzed to a variety of products including isothiocyanates. the current study emphasizes on the antimutagenic potential of three isothiocyanates (itcs) viz. allyl, benzyl and 3-butenyl itcs (individually and in binary combinations) in ...201728506700
tumor necrosis factor receptor-associated factor 6 (traf6) mediates ubiquitination-dependent stat3 activation upon salmonella enterica serovar typhimurium infection.salmonella enterica serovar typhimurium can inject effector proteins into host cells via type iii secretion systems (t3sss). these effector proteins modulate a variety of host transcriptional responses to facilitate bacterial growth and survival. here we show that infection of host cells with s typhimurium specifically induces the ubiquitination of tumor necrosis factor receptor-associated factor 6 (traf6). this traf6 ubiquitination is triggered by the salmonella pathogenicity island 1 (spi-1) t ...201728507064
iron- and hepcidin-independent downregulation of the iron exporter ferroportin in macrophages during salmonella infection.retention of iron in tissue macrophages via upregulation of hepcidin (hamp) and downregulation of the iron exporter ferroportin (fpn) is thought to participate in the establishment of anemia of inflammation after infection. however, an upregulation of fpn has been proposed to limit macrophages iron access to intracellular pathogens. therefore, we studied the iron homeostasis and in particular the regulation of fpn after infection with salmonella enterica serovar typhimurium in mice presenting ti ...201728507548
antibacterial mechanism of the action of enteromorpha linza l. essential oil against escherichia coli and salmonella typhimurium.identification of natural antibacterial agents from various sources that can act effectively against disease causing foodborne bacteria is one of the major concerns throughout the world. however, the natural antibacterial agents identified to date are primarily effective against gram positive bacteria, but less effective against gram negative bacteria. in the present study, enteromorpha linza l. essential oil (eeo) was evaluated for antibacterial activity against escherichia coli and salmonella ...201528510822
effect of intraperitoneal injection of saffron on the treatment of experimental endotoxin induced uveitis in the rabbit.saffron with scientific name of "crocus sativus" from the family "iridaceae" is a plant without stem and root. antioxidative and immunomodulatory effects of saffron has been demonstrated in different studies.201728511420
detecting and discriminating shigella sonnei using an aptamer-based fluorescent biosensor platform.in this paper, a whole-bacteria selex (wb-selex) strategy was adopted to isolate specific aptamers against shigella sonnei. real-time pcr amplification and post-selex experiment revealed that the selected aptmers possessed a high binding affinity and specificity for s. sonnei. of the 21 aptamers tested, the c(t) values of the ss-3 and ss-4 aptamers (ct = 13.89 and ct = 12.23, respectively) had the lowest value compared to other aptamer candidates. the ss-3 and ss-4 aptamers also displayed a bind ...201728513559
phenotypic and genotypic characterization of salmonella spp. isolated from foods and clinical samples in brazil.increasing antimicrobial resistance in salmonella species has been a serious problem for public health worldwide. this study examines salmonella spp. recovered from foods and clinical samples on serotype, antimicrobial resistance and pfge genotypes. it identified 91 salmonellae, belonging to 31 different serotypes, from 36 isolates from food and 55 clinical samples. salmonella infantis (16.5%) and salmonella enteritidis (13.7%) are the most common among food isolates, whereas salmonella enteriti ...201728513778
loss of humoral and cellular immunity to invasive nontyphoidal salmonella during current or convalescent plasmodium falciparum infection in malawian children.invasive nontyphoidal salmonella (ints) infections are commonly associated with plasmodium falciparum infections, but the immunologic basis for this linkage is poorly understood. we hypothesized that p. falciparum infection compromises the humoral and cellular immunity of the host to nts, which increases the susceptibility of the host to ints infection. we prospectively recruited children aged between 6 and 60 months at a community health centre in blantyre, malawi, and allocated them to the fol ...201728515136
zinc supplementation, via gpr39, upregulates pkcζ to protect intestinal barrier integrity in caco-2 cells challenged by salmonella enterica serovar typhimurium.background: zinc has been shown to improve intestinal barrier function against salmonella enterica serovar typhimurium (s. typhimurium) infection, but the mechanisms involved in this process remain undefined.objective: we aimed to explore the roles of g protein-coupled receptor (gpr)39 and protein kinase cζ (pkcζ) in the regulation by zinc of intestinal barrier function.methods: a transwell caco-2 monolayer was pretreated with 0, 50, or 100 μm zn and then incubated with s. typhimurium for 0-6 h. ...201728515165
ca(2+) in hybridization solutions for fluorescence in situ hybridization facilitates the detection of enterobacteriaceae.fluorescence in situ hybridization (fish) has been employed to identify microorganisms at the single cell level under a microscope. extensive efforts have been made to improve and extend the fish technique; however, the development of a widely applicable protocol is a continuing challenge. the present study evaluated the effects of divalent cations in the hybridization solution on the fish-based detection of various species of bacteria and archaea with rrna-targeted probes. a flow cytometric ana ...201728515389
assessment of microbial and radioactive contaminations in korean cold duck meats and electron-beam application for quality improvement.animal-origin food products pose serious threat to public food safety due to high microbial loads. the microbial and radioactive contaminations in commercial cold duck meat products were evaluated. ten different lots of commercial samples (c1-c10) were classified based on type and smoking process. all samples were highly contaminated (< 4-7 log cfu/g) with total aerobic bacteria (tab), yeasts and molds (y&m), and 7 samples (c1-c7) were positive for coliforms. furthermore, three samples were cont ...201728515653
salmonella diva vaccine reduces disease, colonization and shedding due to virulent s. typhimurium infection in swine.non-host-adapted salmonella serovars, including the common human food-borne pathogen salmonella enterica serovar typhimurium (s. typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. interventions against salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (diva).201728516860
genomic analysis of salmonella enterica serovar typhimurium dt160 associated with a 14-year outbreak, new zealand, 1998-2012.during 1998-2012, an extended outbreak of salmonella enterica serovar typhimurium definitive type 160 (dt160) affected >3,000 humans and killed wild birds in new zealand. however, the relationship between dt160 within these 2 host groups and the origin of the outbreak are unknown. whole-genome sequencing was used to compare 109 salmonella typhimurium dt160 isolates from sources throughout new zealand. we provide evidence that dt160 was introduced into new zealand around 1997 and rapidly propagat ...201728516864
gut microbiota-mediated protection against diarrheal infections.the mammalian gut microbiota is a highly abundant and diverse microbial community that resides in the gastrointestinal tract. one major benefit that the gut microbiota provides to its host is colonization resistance-the ability to prevent colonization by foreign microbes, including diarrheal pathogens such as clostridium difficile , salmonella enterica serovar typhimurium and diarrheagenic escherichia coli .201728520994
influence of gsh s-transferase on the mutagenicity induced by dichloromethane and 1,2-dichloropropane.it has been suggested that dichloromethane (dcm) and 1,2-dichloropropane (dcp) are responsible for occupational cholangiocarcinoma. dihaloalkanes are metabolically activated by gsh s-transferase theta1 (gstt1) to yield products such as episulfonium ions. however, whether the gstt1-mediated step of these dihaloalkanes is related to occupational cholangiocarcinoma is not known. in the present study, we investigated the influence of gstt1 activation on the mutagenicity of dcm and 1,2-dcp using gstt ...201728521016
t cell immunity induced by a bivalent salmonella-based ceacam6 and 4-1bbl vaccines in a rat colorectal cancer model.the present study investigated the anti-tumor mechanisms of recombinant non-specific cross-reacting antigen (ceacam6) and 4-1bb ligand (4-1bbl) salmonella-based vaccines, and the effect that these vaccinations have on memory t cells and t helper (th) cell polarization. colon tumors were induced in rats via 1,2-dimethylhydrazine (dmh) injections. rats were then treated with injections of attenuated salmonella typhimurium carrying pires-ceacam6, pires-4-1bbl or pires-ceacam6-4-1bbl. in total, 4 va ...201728521477
amylases and their importance during glycan degradation: genome sequence release of salmonella amylase knockout strains.amylases catalyze the cleavage of α-d-1,4 and α-d-1,6-glycosidic bonds in starch and related carbohydrates. amylases are widely distributed in nature and are important in carbohydrate metabolism. this is the release of four single and two double deletions in salmonella enterica serovar typhimurium lt2 that are important for glycan degradation during infection.201728522713
proteome remodelling by the stress sigma factor rpos/σ(s) in salmonella: identification of small proteins and evidence for post-transcriptional regulation.the rpos/σ(s) sigma subunit of rna polymerase is the master regulator of the general stress response in many gram-negative bacteria. extensive studies have been conducted on σ(s)-regulated gene expression at the transcriptional level. in contrast, very limited information regarding the impact of σ(s) on global protein production is available. in this study, we used a mass spectrometry-based proteomics approach to explore the wide σ(s)-dependent proteome of the human pathogen salmonella enterica ...201728522802
safety evaluation of an oat grain alkaloid gramine by genotoxicity assays.gramine is a natural indole alkaloid that has been isolated from different raw plants occurring mainly in avena sativa, etc. the study was aimed to investigate the possible in vitro antioxidant, in vitro mutagenic, in vitro antimutagenic, and in vivo genotoxic activity of gramine using ferric reducing ability of plasma (frap) assay, metal chelating, ames bacterial reverse mutation test, and the mouse bone marrow micronucleus assay as well as chromosomal aberration. four concentrations of gramine ...201728523965
investigation of optimum ohmic heating conditions for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in apple juice.control of foodborne pathogens is an important issue for the fruit juice industry and ohmic heating treatment has been considered as one of the promising antimicrobial interventions. however, to date, evaluation of the relationship between inactivation of foodborne pathogens and system performance efficiency based on differing soluble solids content of apple juice during ohmic heating treatment has not been well studied. this study aims to investigate effective voltage gradients of an ohmic heat ...201728525985
impact of acanthamoeba cysts on stress resistance of salmonella enterica serovar typhimurium, yersinia enterocolitica 4/o:3, listeria monocytogenes 1/2a, and escherichia coli o:26.the formation of robust resting cysts enables acanthamoeba to resist harsh environmental conditions. this study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cro ...201728526786
getting "inside" type i ifns: type i ifns in intracellular bacterial infections.type i interferons represent a unique and complex group of cytokines, serving many purposes during innate and adaptive immunity. discovered in the context of viral infections, type i ifns are now known to have myriad effects in infectious and autoimmune disease settings. type i ifn signaling during bacterial infections is dependent on many factors including whether the infecting bacterium is intracellular or extracellular, as different signaling pathways are activated. as such, the repercussions ...201728529959
efficacy of disinfectants and detergents intended for a pig farm environment where salmonella is present.disinfection is a useful component of disease control, although products and chemical groups vary in their activity against different pathogens. this study investigated the ability of fifteen disinfectants to eliminate pig-associated salmonella. active compounds of products included chlorocresol, glutaraldehyde/formaldehyde, glutaraldehyde/quaternary ammonium compounds (qac), iodine, peracetic acid and potassium peroxomonosulphate. six detergents were also tested for their ability to dislodge fa ...201728532805
structure and intermolecular interactions between l-type straight flagellar filaments.bacterial mobility is powered by rotation of helical flagellar filaments driven by rotary motors. flagellin isolated from the salmonella typhimurium sjw1660 strain, which differs by a point mutation from the wild-type strain, assembles into straight filaments in which flagellin monomers are arranged in a left-handed helix. using small-angle x-ray scattering and osmotic stress methods, we investigated the structure of sjw1660 flagellar filaments as well as the intermolecular forces that govern th ...201728538155
assessment of mexican arnica (heterotheca inuloides cass) and rosemary (rosmarinus officinalis) extracts on dopamine and selected biomarkers of oxidative stress in stomach and brain of salmonella typhimurium infected rats.the effects of some natural products on dopamine (da) and 5-hydroxyindole acetic acid (5-hiaa) in brain of infected models are still unclear.201728539708
antibiofilm activity and mode of action of dmso alone and its combination with afatinib against gram-negative pathogens.biofilms are complex microbial communities that tend to attach to either biotic or abiotic surface. enclosed in a self-produced extracellular polymeric substance (eps) matrix, the biofilms often cause persistent infections. the objective of this study was to investigate the antibiofilm activity of dimethyl sulfoxide (dmso) and afatinib against gram-negative pathogens. test microorganisms used in this study were escherichia coli atcc 1299, pseudomonas aeruginosa atcc 10145, and salmonella typhimu ...201728540585
quantitative proteomic analysis of host epithelial cells infected by salmonella enterica serovar typhimurium.systems-level analyses have the capability to offer new insight into host-pathogen interactions on the molecular level. using salmonella infection of host epithelial cells as a model system, we previously analyzed intracellular bacterial proteome as a window into pathogens' adaptations to their host environment [infect. immun. 2015; j. proteome res. 2017]. herein we extended our efforts to quantitatively examine protein expression of host cells during infection. in total, we identified more than ...201728544771
two dynamical regimes of the substrate radical rearrangement reaction in b12-dependent ethanolamine ammonia-lyase resolve contributions of native protein configurations and collective configurational fluctuations to catalysis.the kinetics of the substrate radical rearrangement reaction step in b12-dependent ethanolamine ammonia-lyase (eal) from salmonella typhimurium are measured over a 92 k temperature range. the observed first-order rate constants display a piecewise-continuous arrhenius dependence, with linear regions over 295 → 220 k (monoexponential) and 214 → 203 k (biexponential) that are delineated by a kinetic bifurcation and kinks at 219 and 217 k, respectively. the results are interpreted by using a free e ...201728548844
flagellin fljb as an adjuvant to the recombinant adenovirus rabies glycoprotein vaccine increases immune responses against rabies in mice.rabies virus (rabv) causes an acute progressive viral encephalitis. although currently licensed vaccines have an excellent safety and efficacy record, the development of a safer and more cost-effective vaccine is still being sought. an e1-deleted, replication-defective human adenovirus type 5 (had5) vector expressing rabv glycoprotein (had5-g) is thought to be a promising candidate vaccine for immune prophylaxis against rabies. salmonella enterica serovar typhimurium (s. typhimurium) flagellin i ...201728550434
evaluation of genetic toxicity of 6-diazo-5-oxo-l-norleucine (don).don (6-diazo-5-oxo-l-norleucine), a glutamine antagonist, was demonstrated to exhibit analgesic, antibacterial, antiviral and anticancer properties. the study was performed to characterize its in vitro and in vivo genetic toxicity potential. don was tested in the bacterial reverse mutation assay (ames test) using salmonella typhimurium tester strains (ta98, ta100, ta1535 and ta1537) and escherichia coli tester strain (wp2 uvra) with and without s9 and also with reductive s9. in addition, don was ...201728552037
resveratrol induces membrane and dna disruption via pro-oxidant activity against salmonella typhimurium.resveratrol is a flavonoid found in various plants including grapes, which has been reported to be active against various pathogenic bacteria. however, antibacterial effects and mechanisms via pro-oxidant property of resveratrol remain unknown and speculative. this research investigated antibacterial mechanism of resveratrol against a food-borne human pathogen salmonella typhimurium, and confirmed the cell death associated oxidative damage. resveratrol increased outer membrane permeability and m ...201728552524
the in vitro and in vivo protective effects of tannin derivatives against salmonella enterica serovar typhimurium infection.in this study, we investigated the protective effects of tannin-derived components, gallic acid (ga) and tannic acid (ta), in vitro and in vivo against salmonella infection in mice. both ga and ta showed antibacterial effects against salmonella (s.) typhimurium as well as inhibitory effects on the adherence, invasion, and intracellular growth of the pathogens in macrophages. following a lethal dose of salmonella infection in mice, reduced virulence in both ga- and ta-treated groups was observed ...201728552635
effect of high dietary manganese on the immune responses of broilers following oral salmonella typhimurium inoculation.manganese (mn) is an essential nutrient for both host and pathogen. recent studies have demonstrated the nutritional immunity of mn against salmonella infection in mammals. to investigate the effect of high dietary mn on immune responses of broilers following salmonella challenge, 144 1-day-old male broilers were fed a basal diet (containing 20.04 mg mn/kg) plus an additional 40 (the control group) or 400 mg mn/kg (the h-mn group) for 7 days. the 72 broilers in each group were then orally inocul ...201728555440
sugar sulfates are not hydrolyzed by the acid-inducible sulfatase asla from salmonella enterica enteritidis nal(r) and kentucky 3795 at ph 5.5.the open reading frames sen0085 and seka_a4361, from salmonella enterica serovar enteritidis nal(r) and serovar kentucky 3795, respectively, corresponding to the acid-inducible sulfatase gene asla from salmonella enterica serovar typhimurium, were previously suggested by microarray analysis to be differentially expressed under acid conditions. however, growth and enzyme activity tests in the present study demonstrated that both wild-type strains exhibited sulfatase activity with 4-nitrophenyl su ...201728558253
three new lactobacillus plantarum strains in the probiotic toolbox against gut pathogen salmonella enterica serotype typhimurium.the benefits of probiotic bacteria have been widely explored. however, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. in this study we present three new lactobacillus plantarum strains isolated from different dairy products: cow's cheese, sheep's cheese and whey. in order to determine the antibacterial activity of yet unexplored l. plantarum strains against salmonella enterica serotype typhimurium, in vitro comp ...201728559733
salmonella-mediated cancer therapy: roles and potential.the use of bacteria for cancer therapy, which was proposed many years ago, was not recognized as a potential therapeutic strategy until recently. technological advances and updated knowledge have enabled the genetic engineering of bacteria for their safe and effective application in the treatment of cancer. the efficacy of radiotherapy depends mainly on tissue oxygen levels, and low oxygen concentrations in necrotic and hypoxic regions are a common cause of treatment failure. in addition, the di ...201728559936
multidrug resistant invasive nontyphoidal salmonella isolated from and masquerading healed tubercular constrictive pericarditis and study of virulence markers.among the infectious causes of pericarditis are various bacteria, viruses, fungal and parasitic infections. the course disease may progress to a chronic constrictive pattern especially with tubercular etiology. non-typhoidal salmonella has rarely been reported as a cause of pericarditis. we describe here a case from which the pericardial fluid from an old case of tubercular pericarditis sent for culture to microbiology laboratory grew a salmonella typhimurium. we studied the antibiotic resistanc ...201728560124
production of recombinant antimicrobial polymeric protein beta casein-e 50-52 and its antimicrobial synergistic effects assessment with thymol.accelerating emergence of antimicrobial resistance among food pathogens and consumers' increasing demands for preservative-free foods are two contemporary challenging aspects within the food industry. antimicrobial packaging and the use of natural preservatives are promising solutions. in the present study, we used beta-casein-one of the primary self-assembly proteins in milk with a high polymeric film production capability-as a fusion partner for the recombinant expression of e 50-52 antimicrob ...201728561787
differences in carbon source utilization of salmonella oranienburg and saintpaul isolated from river water.long-term exposure to river water by non-indigenous micro-organisms such as salmonella may affect metabolic adaptation to carbon sources. this study was conducted to determine differences in carbon source utilization of salmonella oranienburg and salmonella saintpaul (isolated from tropical river water) as well as the control strain salmonella typhimurium exposed to laboratory, river water, and host cells (hep-2 cell line) growth conditions. results showed that salmonella oranienburg and salmone ...201728565917
genetic structure and diversity within local populations of bacillus mycoides.sixty strains of bacillus mycoides were isolated from each of two sites and characterized by their responses to standard metabolic tests used in bacterial taxonomy, by multilocus enzyme electrophoresis (mlee), and by restriction-fragment-length polymorphism (rflp) analysis of southern blots probed with both a conserved dna fragment derived from a salmonella typhimurium ribosomal cistron and with two cosmid probes derived from b. mycoides atcc strain 6463. both mlee and rflp analyses indicated th ...199428568417
characterization of pertussis-like toxin from salmonella spp. that catalyzes adp-ribosylation of g proteins.salmonella typhimurium definitive phage type (dt) 104 produces a pertussis-like toxin (artab-dt104), which catalyzes adp-ribosylation of pertussis toxin sensitive g proteins. however, the prevalence of artab and its toxicity have not been established. we report here that, in addition to dt104, s. worthington, and s. bongori, produce artab homologs, designated artab-sw and artab-sb, respectively. we purified and characterized these artab toxins, which comprise a 27-kda a subunit (arta) and 13.8-k ...201728572615
signal transduction pathway mediated by the novel regulator loia for low oxygen tension induced salmonella typhimurium invasion.salmonella enterica serovar typhimurium (s. typhimurium) is a major intestinal pathogen of both humans and animals. salmonella pathogenicity island 1 (spi-1)-encoded virulence genes are required for s. typhimurium invasion. while oxygen (o2) limitation is an important signal for spi-1 induction under host conditions, how the signal is received and integrated to the central spi-1 regulatory system in s. typhimurium is not clear. here, we report a signal transduction pathway that activates spi-1 e ...201728575106
characterization of antimicrobial properties of salmonella phage felix o1 and listeria phage a511 embedded in xanthan coatings on poly(lactic acid) films.beyond simply providing a barrier between food and external contaminants, active packaging technologies aim to inhibit pathogen survival and growth within the packaged environment. bacteriophages have a proven track record as targeted antimicrobials but have yet to be successfully integrated in active packaging without serious loss of activity. we have developed two bacteriophage based xanthan coatings on poly(lactic acid) (pla) film which significantly inhibits salmonella typhimurium and lister ...201728576360
factors influencing the salmonella internalization into seedpods and whole plants of arachis hypogaea (l.).here we investigated whether salmonella enterica serovar typhimurium atcc 14028 was capable of internalizing in peanut seedpods and plants when exposed to inoculated soil and the edaphic factors that influenced uptake. intact dry virginia (dv) and fresh green virginia (gv) seedpods were exposed to soil containing 6.5 log (cfu/g) salmonella under different soil moisture conditions. internalization of s. typhimurium into peanut plants germinated in inoculated soil was also examined with and withou ...201728576367
tlr9 regulates the nf-κb-nlrp3-il-1β pathway negatively in salmonella-induced nkg2d-mediated intestinal inflammation.tlrs are key sensors for conserved bacterial molecules and play a critical role in host defense against invading pathogens. although the roles of tlrs in defense against pathogen infection and in maintaining gut immune homeostasis have been studied, the precise functions of different tlrs in response to pathogen infection in the gut remain elusive. the present study investigated the role of tlr signaling in defense against the gram-negative bacterial pathogen salmonella typhimurium the results i ...201728576980
bio-mining the forest ecosystem of north east india for identification of antimicrobial metabolites from fungi through submerged fermentation.in this study, fungi isolated from less explored forest soil ecosystem of northeast india were studied for the production of potential antimicrobial metabolites (amm). out of the 68 fungi isolated from forest soil of manipur, 7 of them showed ama against the test pathogens. among them, aspergillus terreus (ibsd-f4) showed the most significant activity against staphylococcus aureus (atcc-25923), bacillus anthracis (ibsd-c370), pseudomonas fluorescens (atcc-13525), salmonella typhimurium (atcc-140 ...201728578806
exploiting a host-commensal interaction to promote intestinal barrier function and enteric pathogen tolerance.commensal intestinal bacteria can prevent pathogenic infection; however, limited knowledge of the mechanisms by which individual bacterial species contribute to pathogen resistance has restricted their potential for therapeutic application. here, we examined how colonization of mice with a human commensal enterococcus faecium protects against enteric infections. we show that e. faecium improves host intestinal epithelial defense programs to limit salmonella enterica serotype typhimurium pathogen ...201628580440
insights into pg-binding, conformational change, and dimerization of the ompa c-terminal domains from salmonella enterica serovar typhimurium and borrelia burgdorferi.salmonella enterica serovar typhimurium can induce both humoral and cell-mediated responses when establishing itself in the host. these responses are primarily stimulated against the lipopolysaccharide and major outer membrane (om) proteins. ompa is one of these major om proteins. it comprises a n-terminal eight-stranded β-barrel transmembrane domain and a c-terminal domain (ompa(ctd) ). the ompa(ctd) and its homologs are believed to bind to peptidoglycan (pg) within the periplasm, maintaining b ...201728580643
the boosting potential of bacteria in cancer immunotherapy.salmonella typhimurium, engineered to express flagellin b, recently demonstrated unprecedented tumor control through a tlr-dependent mechanism. here, we review new observations that support the potential of utilizing modified bacteria to enhance antitumor immunity. we also discuss the implications of these findings for clinical applications, including immune checkpoint blockade therapies.201728583420
combined treatment of thymus vulgaris l., rosmarinus officinalis l. and myrtus communis l. essential oils against salmonella typhimurium: optimization of antibacterial activity by mixture design methodology.to increase the sensibility of salmonella typhimurium strain, a mixture of thymus vulgaris l. (t. vulgaris l.), rosmarinus officinalis l. (r. officinalis l.) and myrtus communis l. (m. communis l.) essential oils (eos) was used in combined treatment by experimental design methodology (mixture design). the chemical composition of eos was firstly identified by gc and gc/ms and their antibacterial activity was evaluated. the results of this first step have shown that thymol and borneol were the maj ...201728583590
dietary safety of a dual-enzyme preparation for animal feed: acute and subchronic oral toxicity and genotoxicity studies.animal feed is routinely supplemented with exogenous enzymes to improve nutrient utilization, such as proteases to enhance protein hydrolysis in vivo and xylanases to alleviate feed related anti-nutritional factors. the present studies were conducted to evaluate the potential oral toxicity and genotoxicity of a dual-enzyme preparation, vegpro(®) concentrate (vpr-c). acute oral toxicity studies were conducted in adult male and female sprague-dawley crl cd rats and chs swiss ico:ofi (iops caw) mic ...201728583667
mass spectrometry imaging identifies palmitoylcarnitine as an immunological mediator during salmonella typhimurium infection.salmonella typhimurium causes a self-limiting gastroenteritis that may lead to systemic disease. bacteria invade the small intestine, crossing the intestinal epithelium from where they are transported to the mesenteric lymph nodes (mlns) within migrating immune cells. mlns are an important site at which the innate and adaptive immune responses converge but their architecture and function is severely disrupted during s. typhimurium infection. to further understand host-pathogen interactions at th ...201728584281
copper-modified palygorskite is effective in preventing and treating diarrhea caused by salmonella typhimurium.the aim of this research was to develop effective alternative therapies to reduce antibiotic use in animal agriculture. in this study, the efficacy of copper-modified palygorskite (cm-pal) in preventing diarrhea caused by salmonella was specifically examined both in vitro and in vivo. the cm-pal was prepared with palygorskite (pal) and copper nitrate. the antibacterial activity of the cm-pal was detected by comparing the differences in cell numbers on plate count agar before and after adding the ...201728585423
contamination of carcasses and utensils in small swine slaughterhouses by salmonella in the northwestern region of the state of rio de janeiro, brazil.salmonella is a major foodborne pathogen that constantly threatens food safety in developed countries and underdeveloped countries such as brazil, where it is responsible for 38% of notified cases of foodborne illness. swine are one of the main meat-producing species that may asymptomatically carry salmonella, periodically shedding the bacteria through feces. the state of rio de janeiro is not a major producer of swine meat, but small slaughterhouses are operational and produce meat for consumpt ...201728585863
impact of sweet potato starch-based nanocomposite films activated with thyme essential oil on the shelf-life of baby spinach leaves.salmonella typhimurium (s. typhi) and escherichia coli (e. coli) have been responsible for an increasing number of outbreaks linked to fresh produce, such as baby spinach leaves, in the last two decades. more recently, antimicrobial biodegradable packaging systems have been attracting much attention in the food packaging industry as eco-friendly alternatives to conventional plastic packaging. the objective of this study was to evaluate the effect of antibacterial nanocomposite films on inoculate ...201728587199
the rcs-regulated colanic acid capsule maintains membrane potential in salmonella enterica serovar typhimurium.the rcs phosphorelay and psp (phage shock protein) systems are envelope stress responses that are highly conserved in gammaproteobacteria. the rcs regulon was found to be strongly induced during metal deprivation of salmonella enterica serovar typhimurium lacking the psp response. nineteen genes activated by the rcsa-rcsb response regulator make up an operon responsible for the production of colanic acid capsular polysaccharide, which promotes biofilm development. despite more than half a centur ...201728588134
transcriptomic analysis of the swarm motility phenotype of salmonella enterica serovar typhimurium mutant defective in periplasmic glucan synthesis.movement of food-borne pathogens on moist surfaces enables them to migrate towards more favorable niches and facilitate their survival for extended periods of time. salmonella enterica serovar typhimurium mutants defective in osmoregulated periplasmic glucans (opg) synthesis are unable to exhibit motility on moist surfaces (swarming); however, their mobility in liquid (swim motility) remains unaffected. in order to understand the role of opg in swarm motility, transcriptomic analysis was perform ...201728593349
live-attenuated auxotrophic mutant of salmonella typhimurium expressing immunogenic ha1 protein enhances immunity and protective efficacy against h1n1 influenza virus infection.to evaluate the efficacy of attenuated salmonella typhimurium (jol912) as a live bacterial vaccine vector.201728594235
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