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variable coordination and c-s bond cleavage activity of n-substituted imidazolidine-2-thiones towards copper: synthesis, spectroscopy, structures, esi-mass and antimicrobial studies.an equimolar reaction of copper(i) iodide with n-ethyl-imidazolidine-2-thione (l-et) in acetonitrile formed black prismatic crystals over a period of three weeks. the x-ray structure determination of black crystals revealed that the thio-ligand l-et has transformed into a new thio-ligand, 1-ethyl-3-(1-ethyl-4,5-dihydro-1h-imidazol-2-yl)imidazolidine-2-thione (l-net), through c-s rupture/c-n bond formation which was coordinated in the unusual 2d polymer, {(cu(ii)(κ(2)-n,s-l-net)2)·(cui4)·(cui2)}n ...201728070570
promyelocytic leukemia protein (pml) controls listeria monocytogenes infection.the promyelocytic leukemia protein (pml) is the main organizer of stress-responsive subnuclear structures called pml nuclear bodies. these structures recruit multiple interactors and modulate their abundance or their posttranslational modifications, notably by the sumo ubiquitin-like modifiers. the involvement of pml in antiviral responses is well established. in contrast, the role of pml in bacterial infection remains poorly characterized. here, we show that pml restricts infection by the patho ...201728074026
anti-folates potentiate bactericidal effects of other antimicrobial agents.synergies between sulfonamides and other antimicrobial agents have long been reported, but the reason still remains unclear. previously, vilchèze et al. found that, sulfamethoxazole (smx) could potentiate the bacterialcidal activity of isoniazid (inh) and rifampin (rif) in mycobacterium tuberculosis. to test if this was also the case in other bacteria, the ability to potentiate bactericidal effect of rif by smx was evaluated in escherichia coli, staphylococcus aureus, salmonella typhimurium and ...201728074051
isolation and characterization of antimutagenic components of glycyrrhiza aspera against n-methyl-n-nitrosourea.a powdered ethanolic extract of glycyrrhiza aspera root exhibits antimutagenic activity against n-methyl-n-nitrosourea (mnu) based on the ames assay with salmonella typhimurium ta1535. the aim of this study was to identify the antimutagenic components of the powdered ethanolic extract of g. aspera root.201728074112
molecular characterization and antibiotic resistance of salmonella serovars isolated in the apulia region of italy.during the period january 2013-december 2015, 175 cases of human salmonellosis were reported in the apulia region of italy. the aim of this study was to characterize salmonella strains from the standpoints of serovars prevalence, antimicrobial resistance and clonal origin. the serological typing was performed by agglutination against antisera followed by a multiplex polymerase chain reaction (m-pcr). the obtained results were analyzed following the kauffmann-white scheme. susceptibility to antim ...201728078872
smart metal-organic framework coatings: triggered antibiofilm compound release.metal-organic frameworks (mofs) have a large potential for delivery of active molecules. here, a mof coating is investigated as a smart host matrix for triggered release of antibiofilm compounds. in addition to a coating consisting of the regular fe-terephthalate mil-88b(fe), a new hydrophobic mil-88b(fe) coating is synthesized in hydrothermal conditions using palmitic acid as a lattice terminating group. these porous materials are used as a host matrix for the antibiofilm compound 5-(4-chloroph ...201728081368
evolution of variable number tandem repeats and its relationship with genomic diversity in salmonella typhimurium.salmonella enterica serovar typhimurium is the most common salmonella serovar causing human infections in australia and many other countries. a total of 12,112 s. typhimurium isolates from new south wales were analyzed by multi-locus variable number of tandem repeat (vntr) analysis (mlva) using five vntrs from 2007 to 2014. we found that mid ranges of repeat units of 8-14 in vntr locus sttr5, 6-13 in sttr6, and 9-12 in sttr10 were always predominant in the population (>50%). in vitro passaging e ...201628082952
structural basis for the recognition and degradation of host trim proteins by salmonella effector sopa.the hallmark of salmonella typhimurium infection is an acute intestinal inflammatory response, which is mediated through the action of secreted bacterial effector proteins. the pro-inflammatory salmonella effector sopa is a hect-like e3 ligase, which was previously proposed to activate host ring ligases trim56 and trim65. here we elucidate an inhibitory mechanism of trim56 and trim65 targeting by sopa. we present the crystal structure of sopa in complex with the ring domain of human trim56, reve ...201728084320
differential susceptibility and maturation of thymocyte subsets during salmonella typhimurium infection: insights on the roles of glucocorticoids and interferon-gamma.the thymus is known to atrophy during infections; however, a systematic study of changes in thymocyte subpopulations has not been performed. this aspect was investigated, using multi-color flow cytometry, during oral infection of mice with salmonella typhimurium (s. typhimurium). the major highlights are: first, a block in the developmental pathway of cd4(-)cd8(-) double negative (dn) thymocytes is observed. second, cd4(+)cd8(+) double positive (dp) thymocytes, mainly in the dp1 (cd5(lo)cd3(lo)) ...201728091621
mplex: a method for simultaneous pathogen inactivation and extraction of samples for multi-omics profiling.the continued emergence and spread of infectious agents is of great concern, and systems biology approaches to infectious disease research can advance our understanding of host-pathogen relationships and facilitate the development of new therapies and vaccines. molecular characterization of infectious samples outside of appropriate biosafety containment can take place only subsequent to pathogen inactivation. herein, we describe a modified folch extraction using chloroform/methanol that facilita ...201728091625
functional constituents of wild and cultivated goji (l. barbarum l.) leaves: phytochemical characterization, biological profile, and computational studies.goji (lycium barbarum l.) leaves are emphasized as a functional tea or as dietary supplements. the phenolic compound profile, antioxidant, enzyme inhibitory, antimicrobial, and antimutagenic activities of leaf extracts from two selected cultivars in comparison with wild-growing plants have been evaluated. hplc-dad/esi-tof-ms analysis revealed the presence of phenolic acids and flavonoids with chlorogenic acid and rutin being the dominant compounds in the cultivated plants, whereas rutin and kaem ...201728095717
bacterium-derived cell-penetrating peptides deliver gentamicin to kill intracellular pathogens.commonly used antimicrobials show poor cellular uptake and often have limited access to intracellular targets, resulting in low antimicrobial activity against intracellular pathogens. an efficient delivery system to transport these drugs to the intracellular site of action is needed. cell-penetrating peptides (cpps) mediate the internalization of biologically active molecules into the cytoplasm. here, we characterized two cpps, α1h and α2h, derived from the yersinia enterocolitica yopm effector ...201728096156
phenotypic and genotypic characterization of locally isolated salmonella strains used in preparation of salmonella antigens in egypt.this work was conducted to study the phenotypic and genotypic characterization of locally isolated salmonella strains (salmonella pullorum, salmonella enteritidis, and salmonella typhimurium) from poultry used in the preparation of salmonella antigens in egypt.201628096617
mcr-1-harboring salmonella enterica serovar typhimurium sequence type 34 in pigs, china.we detected the mcr-1 gene in 21 (14.8%) salmonella isolates from pigs at slaughter; 19 were serovar typhimurium sequence type 34. the gene was located on inchi2-like plasmids that also harbored incf replicons and lacked a conjugative transfer region. these findings highlight the need to prevent further spread of colistin resistance in animals and humans.201728098547
tumor-targeting salmonella typhimurium a1-r sensitizes melanoma with a braf-v600e mutation to vemurafenib in a patient-derived orthotopic xenograft (pdox) nude mouse model.previously, a braf-v600e-mutant melanoma obtained from the right chest wall of a patient was grown orthotopically in the right chest wall of nude mice to establish a patient-derived orthotopic xenograft (pdox) model. trametinib (tra), an mek inhibitor, caused tumor regression. in contrast, another mek inhibitor, cobimetinib (cob) could slow but not arrest growth or cause regression of the melanoma pdox. first-line therapy temozolomide (tem) could slow but not arrest tumor growth or cause regress ...201728106277
a novel aptasensor for the colorimetric detection of s. typhimurium based on gold nanoparticles.a simple, fast and convenient colorimetric aptasensor was fabricated for the detection of salmonella typhimurium (s. typhimurium) which was based on the color change effect of gold nanoparticles (gnps). s. typhimurium is one of the most common causes of food-associated disease. aptamers with specific recognition toward s. typhimurium was modified to the surface of prepared gnps. they play a role for the protection of gnps from aggregation toward high concentrations of nacl. with the addition of ...201728107686
a chemiluminescent aptasensor based on rolling circle amplification and co(2+)/n-(aminobutyl)-n-(ethylisoluminol) functional flowerlike gold nanoparticles for salmonella typhimurium detection.a sensitive steady-state chemiluminescent aptasensor based on rolling circle amplification (rca) was fabricated for the detection of salmonella typhimurium. the sensor utilized aptamer modified fe3o4 magnetic nanoparticles (mnps) as capture probes, aptamer as recognition molecules, and co(2+) enhanced n‑(aminobutyl)-n-(ethylisoluminol) (abei) functional flowerlike gold nanoparticles (aunfs) and complementary strand (cdna) complex (co(2+)/abei-aunfs-cdna) as signal probes. and p-iodophenol (pip) ...201728107930
from the cover: an investigation of the genotoxicity and interference of gold nanoparticles in commonly used in vitro mutagenicity and genotoxicity assays.the suitability of 4 in vitro assays, commonly used for mutagenicity and genotoxicity assessment, was investigated in relation to treatment with 14 nm citrate-stabilized gold nanoparticles (aunps). specifically, the ames test was conducted without metabolic activation, where no mutagenic effects were observed. high resolution transmission electron microscopy and cytoviva dark-field image analysis showed that aunps did not enter the bacterial cells, thus confirming the unreliability of the ames t ...201728108664
the bactericidal lectin regiiiβ prolongs gut colonization and enteropathy in the streptomycin mouse model for salmonella diarrhea.the bactericidal lectin regiiiβ is inducibly produced by intestinal epithelial cells as a defense against infection by enteropathogens. in the gut lumen, regiiiβ kills not only certain enteropathogens, but also some commensal bacteria; thus, regiiiβ is also thought to be an innate immune effector shaping microbiota composition and establishing intestinal homeostasis. using the streptomycin mouse model for salmonella colitis, we show that regiiiβ can promote sustained gut colonization of salmonel ...201728111202
hyperforin exhibits antigenotoxic activity on human and bacterial cells.hyperforin (hf), a substance that accumulates in the leaves and flowers of hypericum perforatum l. (st. john's wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. hf exhibits several medicinal properties and is mainly used as an antidepressant. so far, the antigenotoxicity of hf has not been investigated at the level of primary genetic damage, gene mutations, and chromosome aberrations, simultaneously. the present work is designed to investigate the potential antigenoto ...201728117734
the role of universal stress proteins in bacterial infections.universal stress proteins are ubiquitously expressed in bacteria, archaea and plants and other eukaryotes. a general property of usps is their role in adaptation of bacteria to oxidative stress, high temperature, low ph and/or hypoxia. there is increasing evidence that these proteins facilitate the adaption of bacterial pathogens to the human host environment, thereby facilitating colonisation and pathogenicity. usps in mycobacterium tuberculosis are well studied and may play a role in latency o ...201728120710
'obligate' anaerobic salmonella strain yb1 suppresses liver tumor growth and metastasis in nude mice.the antitumor properties of bacteria have been demonstrated over the past decades. however, the efficacy is limited and unclear. furthermore, systemic infection remains a serious concern in bacteria treatment. in this study, the effect of yb1, a rationally designed 'obligate' anaerobic salmonella typhimurium strain, on liver tumor growth and metastasis in a nude mouse orthotopic liver tumor model was investigated. the orthotopic liver tumor model was established in nude mice using the hepatocell ...201728123538
evaluation of genotoxic and mutagenic activity of organic extracts from drinking water sources.an increasing number of industrial, agricultural and commercial chemicals in the aquatic environment lead to various deleterious effects on organisms, which is becoming a serious global health concern. in this study, the ames test and sos/umu test were conducted to investigate the potential genotoxicity and mutagenicity caused by organic extracts from drinking water sources. organic content of source water was extracted with xad-2 resin column and organic solvents. four doses of the extract equi ...201728125725
design, synthesis and spectroscopic characterization of metal (ii) complexes derived from a tetradentate macrocyclic ligand: study on antimicrobial and antioxidant capacity of complexes.the paper presents the synthesis of co(ii), ni(ii) and cu(ii) complexes of macrocyclic schiff base ligand derived from 1, 4-dicarbonyl-phenyl-dihydrazide and ethyl 3-oxobutanoate (2:2). the synthesized ligand and its metal complexes were characterized by elemental analyses, magnetic susceptibility measurements, ftir, uv-vis., mass (1)h nmr and x-ray diffraction. the cu(ii) complex exhibit distorted octahedral geometry, whereas an octahedral geometry is suggested for other complexes. the synthesi ...201728126666
stevia-based sweeteners as a promising alternative to table sugar: the effect on listeria monocytogenes and salmonella typhimurium growth dynamics.sugar is commonly substituted with stevia-based products in food industry and in our daily-life. this substitution results in a change in food product characteristic formula and properties that may affect the growth dynamics of food pathogenic and spoilage bacteria. this work studies the effect of table sugar (ts), laboratory sucrose (ls), commercial stevia (st) and steviol glycosides (sg) on the growth dynamics of salmonella typhimurium and listeria monocytogenes. experiments were carried out i ...201728126702
silver ion-mediated killing of a food pathogen: melting curve analysis data of silver resistance genes and growth curve data.limited antibacterial activity of silver ions leached from silver-impregnated food contact materials could be due to: 1) the presence of silver resistance genes in tested bacteria; or 2) lack of susceptibility to silver ion-mediated killing in the bacterial strain (k. williams, l. valencia, k. gokulan, r. trbojevich, s. khare, 2016 [1]). this study contains data to address the specificity of silver resistance genes in salmonella typhimurium during the real time pcr using melting curve analysis a ...201728127583
pathogen-mediated inhibition of anorexia promotes host survival and transmission.sickness-induced anorexia is a conserved behavior induced during infections. here, we report that an intestinal pathogen, salmonella typhimurium, inhibits anorexia by manipulating the gut-brain axis. inhibition of inflammasome activation by the s. typhimurium effector, slrp, prevented anorexia caused by il-1β-mediated signaling to the hypothalamus via the vagus nerve. rather than compromising host defenses, pathogen-mediated inhibition of anorexia increased host survival. slrp-mediated inhibitio ...201728129542
novel aldo-keto reductases for the biocatalytic conversion of 3-hydroxybutanal to 1,3-butanediol: structural and biochemical studies.the nonnatural alcohol 1,3-butanediol (1,3-bdo) is a valuable building block for the synthesis of various polymers. one of the potential pathways for the biosynthesis of 1,3-bdo includes the biotransformation of acetaldehyde to 1,3-bdo via 3-hydroxybutanal (3-hb) using aldolases and aldo-keto reductases (akrs). this pathway requires an akr selective for 3-hb, but inactive toward acetaldehyde, so it can be used for one-pot synthesis. in this work, we screened more than 20 purified uncharacterized ...201728130301
assessment of the genotoxicity of the tyrosine kinase inhibitor imatinib mesylate in cultured fish and human cells.the selective tyrosine kinase inhibitor imatinib mesylate (im) is a widely used anticancer drug. recent studies showing that im can induce dna and chromosomal damage in crustaceans and higher plants prompted us to re-examine its potential genotoxicity. im was not mutagenic in the ames assay (salmonella typhimurium). cytotoxicity and genotoxicity were evaluated in vitro in zebrafish (danio rerio) liver (zfl), human hepatoma (hepg2), and human peripheral blood lymphocyte (hpbl) cells. genotoxicity ...201728137363
the mechanism of killing by the proline-rich peptide bac7(1-35) against clinical strains of pseudomonas aeruginosa differs from that against other gram-negative bacteria.pseudomonas aeruginosa infections represent a serious threat to worldwide health. proline-rich antimicrobial peptides (pr-amps), a particular group of peptide antibiotics, have demonstrated in vitro activity against p. aeruginosa strains. here we show that the mammalian pr-amp bac7(1-35) is active against some multidrug-resistant cystic fibrosis isolates of p. aeruginosa by confocal microscopy and cytometric analyses, we investigated the mechanism of killing against p. aeruginosa strain pao1 and ...201728137800
effect of chitosan physical form on its antibacterial activity against pathogenic bacteria.the antibacterial activity of chitosan (cs) nanospheres, in comparison with other physical forms, was investigated against salmonella enterica serovar typhimurium and staphylococcus aureus, which are 2 foodborne harmful pathogens. results showed that the antibacterial efficacy of cs nanospheres: (1) was superior to that displayed by cs in powder and solution form; (2) was higher against s. aureus than against salmonella typhimurium; and (3) was dependent on the temperature and ph of the medium d ...201728140469
a novel salmonella strain inactivated by a regulated autolysis system and expressing the b subunit of shiga toxin 2e efficiently elicits immune responses and confers protection against virulent stx2e-producing escherichia coli.salmonella typhimurium (s. typhimurium) inactivated by a regulated autolysis system was genetically engineered to express the homo-pentameric b subunit of shiga toxin 2e (stx2eb) on its surface. to prepare a strain able to yield autolyzed salmonella bearing stx2eb, the plasmid pjhl184 harboring stx 2eb gene was transformed into the attenuated s. typhimurium strain, jol1454. stx2eb subcloned into the antigen delivery cassette of the plasmid was expressed as fusion protein with the outer membrane ...201728143511
the nuclear receptor lxr limits bacterial infection of host macrophages through a mechanism that impacts cellular nad metabolism.macrophages exert potent effector functions against invading microorganisms but constitute, paradoxically, a preferential niche for many bacterial strains to replicate. using a model of infection by salmonella typhimurium, we have identified a molecular mechanism regulated by the nuclear receptor lxr that limits infection of host macrophages through transcriptional activation of the multifunctional enzyme cd38. lxr agonists reduced the intracellular levels of nad(+) in a cd38-dependent manner, c ...201728147278
detailed analysis of c-di-gmp mediated regulation of csgd expression in salmonella typhimurium.the secondary messenger cyclic di-gmp promotes biofilm formation by up regulating the expression of csgd, encoding the major regulator of rdar biofilm formation in salmonella typhimurium. the ggdef/eal domain proteins regulate the c-di-gmp turnover. there are twenty- two ggdef/eal domain proteins in the genome of s. typhimurium. in this study, we dissect the role of individual ggdef/eal proteins for csgd expression and rdar biofilm development.201728148244
anti-quorum sensing and anti-biofilm activity of amomum tsaoko (amommum tsao-ko crevost et lemarie) on foodborne pathogens.cell-to-cell communication or quorum sensing (qs) leads to biofilm formation and causing other virulence factors which are extreme problems for food safety, biofilm related infectious diseases etc. this study evaluated the anti-qs activity of the amomum tsaoko extract (0.5-4 mg/ml) by using chromobacterium violaceum a biosensor strain and biofilm formation by crystal violate assay. experimental results demonstrated that the overall yield of amomum tsao-ko extract was 11.33 ± 0.3% (w/w). mic for ...201728149169
epigenetic regulation of nitric oxide synthase 2, inducible (nos2) by nlrc4 inflammasomes involves parp1 cleavage.nitric oxide synthase 2, inducible (nos2) expression is necessary for the microbicidal activity of macrophages. however, nos2 over-activation causes multiple inflammatory disorders, suggesting a tight gene regulation is necessary. using cytosolic flagellin as a model for inflammasome-dependent nos2 activation, we discovered a surprising new role for nlrc4/caspase-1 axis in regulating chromatin accessibility of the nos2 promoter. we found that activation of two independent mechanisms is necessary ...201728150715
quantitative proteomics charts the landscape of salmonella carbon metabolism within host epithelial cells.we performed a proteomic survey of salmonella enterica serovar typhimurium during infection of host epithelial cells. our data reveal substantial metabolic reshuffling of salmonella in the host in addition to severe degeneration of bacterial flagella and chemotaxis systems. the large-scale quantitative data allowed us to chart an overview of intracellular salmonella carbon metabolism. notably, we found preferential utilization of glycolysis, the pentose phosphate pathway, mixed acid fermentation ...201728152601
draft genome sequence of the tumor-targeting salmonella enterica serovar typhimurium strain sl7207.salmonella enterica serovar typhimurium strain sl7207 is a genetically modified derivative of strain sl1344, which preferentially accumulates in tumors and can be used as a vehicle for tissue-specific gene delivery in vivo here, we report the draft genome sequence of sl7207, confirming a purported aroa deletion and four single-nucleotide polymorphisms compared to sl1344.201728153911
[study of the prevalence of nosocomial infections and associated factors in the two university hospitals of lubumbashi, democratic republic of congo].to estimate the prevalence "on any given day" of nosocomial infections and to determine their associated factors. then, to estimate the prevalence of microorganisms responsible for nosocomial infections in lubumbashi, democratic republic of congo.201628154630
sensory neuron regulation of gastrointestinal inflammation and bacterial host defence.sensory neurons in the gastrointestinal tract have multifaceted roles in maintaining homeostasis, detecting danger and initiating protective responses. the gastrointestinal tract is innervated by three types of sensory neurons: dorsal root ganglia, nodose/jugular ganglia and intrinsic primary afferent neurons. here, we examine how these distinct sensory neurons and their signal transducers participate in regulating gastrointestinal inflammation and host defence. sensory neurons are equipped with ...201728155242
towards an understanding of salmonella enterica serovar typhimurium persistence in swine.salmonella enterica is an important food borne pathogen that is frequently carried by swine. carrier animals pose a food safety risk because they can transmit s. enterica to finished food products in the processing plant or by contamination of the environment. environmental contamination has become increasingly important as non-animal foods (plant-based) have been implicated as sources of s. enterica. the prevalence of s. enterica in swine is high and yet carrier animals remain healthy. s. enter ...201628155802
live-cell imaging of salmonella typhimurium interaction with zebrafish larvae after injection and immersion delivery methods.the zebrafish model has been used to determine the role of vertebrate innate immunity during bacterial infections. here, we compare the in vivo immune response induced by gfp-tagged salmonella typhimurium inoculated by immersion and microinjection in transgenic zebrafish larvae. our novel infection protocols in zebrafish allow live-cell imaging of salmonella colonization.201728161588
potential anti-inflammatory, antioxidant and antimicrobial activities of sambucus australis.sambucus australis cham. & schltdl. (adoxaceae) is used in brazilian folk medicine to treat inflammatory disorders.201728166708
correlating bacterial shedding with fecal corticosterone levels and serological responses from layer hens experimentally infected with salmonella typhimurium.salmonella enteriditis and salmonella typhimurium are commonly isolated during egg-related outbreaks of salmonellosis and represent a significant international public health issue. in australia, salmonella typhimurium is the most common serovar identified in egg product related foodborne outbreaks. while a number of studies have investigated salmonella shedding and host responses to infection, they have been conducted over a short time period. the present study sought to characterise bacterial s ...201728166810
ca(2+)-dependent focal exocytosis of golgi-derived vesicles helps phagocytic uptake in macrophages.the role of golgi apparatus during phagocytic uptake by macrophages has been ruled out in the past. notably, all such reports were limited to fcγ receptor-mediated phagocytosis. here, we unravel a highly devolved mechanism for recruitment of golgi-derived secretory vesicles during phagosome biogenesis, which was important for uptake of most cargos, except the igg-coated ones. we report recruitment of mannosidase-ii-positive golgi-derived vesicles during uptake of diverse targets, including latex ...201728174296
ceruloplasmin-derived peptide is the strongest regulator of oxidative stress and leukotriene synthesis in neutrophils.ceruloplasmin, an acute-phase protein, can affect the activity of leukocytes through its various enzymatic activities and protein-protein interactions (with lactoferrin, myeloperoxidase, eosinophil peroxidase, serprocidins, and 5-lipoxygenase (5-lox), among others). however, the molecular mechanisms of ceruloplasmin activity are not clearly understood. in this study, we tested the ability of two synthetic peptides, rpylkvfnpr (883-892) (p1) and rrpylkvfnprr (882-893) (p2), corresponding to the i ...201728177760
antimicrobial activities of phenolic extracts derived from seed coats of selected soybean varieties.soybean hulls or seed coats consist of complex carbohydrates, proteins, lipids, and polyphenols such as anthocyanidins, proanthocyanidins, and isoflavones. the polyphenolics in the seed coats give them various colors such as black, brown, green, yellow, or even a mottled appearance. in this study, the antimicrobial effects of phenolic extracts from the seed coats of different colored soybeans (yellow, dark brown, brown, and black) were evaluated against foodborne pathogens such as salmonella typ ...201728178372
self-oligomerizing structure of the flagellar cap protein flid and its implication in filament assembly.flid is a self-oligomerizing structural protein that caps the growing end of the bacterial flagellar filament. flid also plays a key role in the flagellar system by continuously adding a new flagellin protein to the tip of the filament. to structurally characterize flid oligomerization and to provide a flid-mediated flagellin polymerization mechanism, we have determined the crystal structures of flid proteins from escherichia coli and salmonella enterica serovar typhimurium (ecflid and stflid, r ...201728179186
two-step enhanced cancer immunotherapy with engineered salmonella typhimurium secreting heterologous flagellin.we report a method of cancer immunotherapy using an attenuated salmonella typhimurium strain engineered to secrete vibrio vulnificus flagellin b (flab) in tumor tissues. engineered flab-secreting bacteria effectively suppressed tumor growth and metastasis in mouse models and prolonged survival. by using toll-like receptor 5 (tlr5)-negative colon cancer cell lines, we provided evidence that the flab-mediated tumor suppression upon bacterial colonization is associated with tlr5-mediated host react ...201728179508
elongation factor p restricts salmonella's growth by controlling translation of a mg(2+) transporter gene during infection.when a ribosome translates mrna sequences, the ribosome often stalls at certain codons because it is hard to translate. consecutive proline codons are such examples that induce ribosome stalling and elongation factor p (ef-p) is required for the stalled ribosome to continue translation at those consecutive proline codons. we found that ef-p is required for translation of the mgtb gene encoding a mg(2+) transporter in the mgtcbr virulence operon from the intracellular pathogen salmonella enterica ...201728181542
modeling the potential for vaccination to diminish the burden of invasive non-typhoidal salmonella disease in young children in mali, west africa.in sub-saharan africa, systematic surveillance of young children with suspected invasive bacterial disease (e.g., septicemia, meningitis) has revealed non-typhoidal salmonella (nts) to be a major pathogen exhibiting high case fatality (~20%). where infant vaccination against haemophilus influenzae type b (hib) and streptococcus pneumoniae has been introduced to prevent invasive disease caused by these pathogens, as in bamako, mali, their burden has decreased markedly. in parallel, nts has become ...201728182657
draft genome sequences of two strains of salmonella enterica serovar typhimurium displaying different virulence in an experimental chicken model.salmonella enterica serovar typhimurium strains 22495 and 22792, obtained from wild birds, were found to display different virulence attributes in an experimental chicken model. closed genome sequences were assembled after sequencing with the roche 454 and illumina miseq platforms. an additional plasmid was present in the more virulent strain 22495.201728183752
chemical cell lysis system applicable to lab-on-a-disc.the design and fabrication of a heating system has been a significant challenge in implementing chemical lysis on a lab-on-a-disc (lod). the proposed system contains a sample inlet, phase change material (pcm) array, heating chamber, and valve in a single disc, providing cost-effective, rapid, and fully automated chemical cell lysis. compared to the conventional cell lysis system, our cell lysis system has many advantages, such as a compact structure that is easily integrated into the lod and re ...201728185056
salmonella effector sopd2 interferes with rab34 function.many intracellular pathogens have evolved highly specialized mechanisms to isolate themselves from the host cell's innate immune response while still obtaining the necessary nutrients to survive. salmonella utilizes type 3 secretion systems (t3sss) to deliver bacterial proteins called effectors, across the encompassing salmonella containing vacuole (scv) membrane, to subvert the host's membrane trafficking pathways and alter other cellular processes. the salmonella pathogenicity island (spi)-2 e ...201728185347
enhanced immune response to a dual-promoter anti-caries dna vaccine orally delivered by attenuated salmonella typhimurium.the strength of immune responses induced by dna vaccine is closely associated with the expression level of cloned antigens available to the antigen presenting cells (apcs). to acquire a larger and more persistent amount of antigen, a dual-promoter, which could double the target antigen output through its expression both in prokaryotic and eukaryotic cells, was employed in the constructed anti-caries dna vaccine with attenuated salmonella as mucosal delivery vector in this study. here, both cmv a ...201728187901
management of salmonella typhimurium sepsis with thoracic infectious aortitis using (18)f-fdg pet/ct. 201728188443
probiotic potential of a lactobacillus bacterium of canine faecal-origin and its impact on select gut health indices and immune response of dogs.the objective of the present study was to develop a probiotic of canine-origin for its potential application in pet nutrition. accordingly, 32 lactic acid bacteria (lab) strains were isolated from faeces of dogs, out of which 9 strains were short-listed for further in vitro testing based on the aggregation time and cell surface hydrophobicity. the results of acid-, bile- and phenol-tolerance tests indicated that out of the nine, isolate cpro23 was having better resistance to these adverse condit ...201728188477
synthesis of l-cysteine derivatives containing stable sulfur isotopes and application of this synthesis to reactive sulfur metabolome.cysteine persulfide is an l-cysteine derivative having one additional sulfur atom bound to a cysteinyl thiol group, and it serves as a reactive sulfur species that regulates redox homeostasis in cells. here, we describe a rapid and efficient method of synthesis of l-cysteine derivatives containing isotopic sulfur atoms and application of this method to a reactive sulfur metabolome. we used bacterial cysteine syntheses to incorporate isotopic sulfur atoms into the sulfhydryl moiety of l-cysteine. ...201728189853
seaweed extracts and galacto-oligosaccharides improve intestinal health in pigs following salmonella typhimurium challenge.pork and pork products are recognised as vehicles of salmonella typhimurium infection in humans. seaweed-derived polysaccharides (swe) and galacto-oligosaccharides (gos) have shown to exhibit antimicrobial, prebiotic and immunomodulatory activity. the objective of this study was to assess the effects of dietary gos and swe supplementation on reducing s. typhimurium numbers and intestinal inflammation in vivo. in total, 30 pigs (n=10/treatment, bw 30.9 kg) were randomly assigned to three dietary ...201728190419
plant lectins conbr and cfl modulate expression toll-like receptors, pro-inflammatory cytokines and reduce the bacterial burden in macrophages infected with salmonella enterica serovar typhimurium.plant lectins have long been used in biomedical research as immunomodulators against tumor cells and microbial infections.201728190471
sonographically detected thickened small bowel in a case of paediatric salmonella typhimurium enteritis.introduction: a five-year-old year old girl developed fever of 40.3° c, vomiting, mild general abdominal pain, followed by bloody diarrhea. description: ultrasonography revealed a length of symmetrically thickened terminal ileum, more than 20 cm long, with walls greater than 0.5 cm containing a long narrow lumen < 1 mm wide. conclusion: given the difficulty of diagnosing the cause of enteritis in young children, ultrasonography represents a simple, non invasive technique which may demonstrate su ...201428191208
salmonella typhimurium disrupts sirt1/ampk checkpoint control of mtor to impair autophagy.during intracellular infections, autophagy significantly contributes to the elimination of pathogens, regulation of pro-inflammatory signaling, secretion of immune mediators and in coordinating the adaptive immune system. intracellular pathogens such as s. typhimurium have evolved mechanisms to circumvent autophagy. however, the regulatory mechanisms targeted by s. typhimurium to modulate autophagy have not been fully resolved. here we report that cytosolic energy loss during s. typhimurium infe ...201728192515
rational design of reversible inhibitors for trehalose 6-phosphate phosphatases.in some organisms, environmental stress triggers trehalose biosynthesis that is catalyzed collectively by trehalose 6-phosphate synthase, and trehalose 6-phosphate phosphatase (t6pp). t6pp catalyzes the hydrolysis of trehalose 6-phosphate (t6p) to trehalose and inorganic phosphate and is a promising target for the development of antibacterial, antifungal and antihelminthic therapeutics. herein, we report the design, synthesis and evaluation of a library of aryl d-glucopyranoside 6-sulfates to se ...201728192710
salmonella proteomics under oxidative stress reveals coordinated regulation of antioxidant defense with iron metabolism and bacterial virulence.salmonella typhimurium is a bacterial pathogen that can cause widespread gastroenteritis. salmonella encounters reactive oxygen species both under free-living conditions and within their mammalian host during infection. to study its response to oxidative stress, we performed the first large-scale proteomic profiling of salmonella upon exposure to h2o2. among 1600 detected proteins, 83 proteins showed significantly altered abundance. interestingly, only a subset of known antioxidants was induced, ...201728193547
prevalence and molecular characterization of mcr-1-positive salmonella strains recovered from clinical specimens in china.the recently discovered colistin resistance-encoding element, mcr-1, adds to the list of antimicrobial resistance genes that rapidly erodes the antimicrobial efficacy of not only the commonly used antibiotics, but also the last line agents of carbapenems and colistin. this study investigated the prevalence of the mobile colistin resistance determinant mcr-1 in salmonella strains recovered from clinical settings in china, and the transmission potential of mcr-1-bearing mobile elements harbored by ...201728193662
prevalence and characterization of salmonella among humans in ghana.non-typhoidal salmonella (nts) is a public health problem worldwide and particularly in africa with high disease burden. this study characterized salmonella isolates from humans in ghana to determine serovar distribution, phage types, and antimicrobial resistance. further, the clonal relatedness among isolates was determined.201728194090
salmonella typhimurium bacteraemia complicated by meningitis and brain abscess in a 3-month-old boy. 201728194898
sumoylation as an integral mechanism in bacterial infection and disease progression.post translational modification pathways regulate fundamental processes of cells and thus govern vital functions. among these, particularly the modification with small ubiquitin-like modifiers (sumo) is being recognized as a pathway crucial for cell homeostasis and health. understandably, bacterial pathogens intervene with the sumo pathway of the host for ensuring successful infection. among the bacterial pathogens known to target host sumoylation varied points of intervention are utilized. majo ...201728197924
attachment of 13 types of foodborne bacteria to jalapeño and serrano peppers and antibacterial effect of roselle calyx extracts, sodium hypochlorite, colloidal silver, and acetic acid against these foodborne bacteria on peppers.chili peppers are a very important crop in mexico. however, these peppers have been associated with salmonella infection outbreaks in the united states, and salmonella and diarrheagenic escherichia coli pathotypes have been isolated from jalapeño and serrano peppers in mexico. to decrease microbial contamination of fruits and vegetables, chemical agents are commonly used; however, chemical agents used to eliminate pathogenic bacteria on vegetables have a limited antimicrobial effect. roselle ( h ...201728199144
nontyphoidal salmonella gastroenteritis in baoshan, shanghai, china, 2010 to 2014: an etiological surveillance and case-control study.nontyphoidal salmonella (nts) gastroenteritis is a widespread global foodborne disease. to identify the epidemiologic characteristics, sources of food contamination, and risk factors of nts gastroenteritis, epidemiologic data and stool specimens of diarrheal patients were collected from sentinel hospitals in baoshan, shanghai, people's republic of china, between 2010 and 2014. food products from nearby farmers' markets and animal feces from live poultry markets and livestock farms were sampled t ...201728207307
absence of genotoxic effects of the chalcone (e)-1-(2-hydroxyphenyl)-3-(4-methylphenyl)-prop-2-en-1-one) and its potential chemoprevention against dna damage using in vitro and in vivo assays.the chalcone (e)-1-(2-hydroxyphenyl)-3-(4-methylphenyl)-prop-2-en-1-one), or 2hmc, displays antileishmanial, antimalarial, and antioxidant activities. the aim of this study was to investigate the cytotoxic, genotoxic, mutagenic, and protective effects of 2hmc using the ames mutagenicity test, the mouse bone marrow micronucleus test, and the comet assay in mice. in the assessment using the ames test, 2hmc did not increase the number of his+ revertants in salmonella typhimurium strains, demonstrat ...201728207781
two dechlorinated chlordecone derivatives formed by in situ chemical reduction are devoid of genotoxicity and mutagenicity and have lower proangiogenic properties compared to the parent compound.chlordecone (cld) is a chlorinated hydrocarbon insecticide, now classified as a persistent organic pollutant. several studies have previously reported that chronic exposure to cld leads to hepatotoxicity, neurotoxicity, raises early child development and pregnancy complications, and increases the risk of liver and prostate cancer. in situ chemical reduction (iscr) has been identified as a possible way for the remediation of soils contaminated by cld. in the present study, the objectives were (i) ...201728210952
regulation of nrf operon expression in pathogenic enteric bacteria: sequence divergence reveals new regulatory complexity.the escherichia coli k-12 nrf operon encodes a periplasmic nitrite reductase, the expression of which is driven from a single promoter, pnrf. expression from pnrf is activated by the fnr transcription factor in response to anaerobiosis and further increased in response to nitrite by the response regulator proteins, narl and narp. fnr-dependent transcription is suppressed by the binding of two nucleoid associated proteins, ihf and fis. as fis levels increase in cells grown in rich medium, the pos ...201728211111
detection of salmonella typhimurium on spinach using phage-based magnetoelastic biosensors.phage-based magnetoelastic (me) biosensors have been studied as an in-situ, real-time, wireless, direct detection method of foodborne pathogens in recent years. this paper investigates an me biosensor method for the detection of salmonella typhimurium on fresh spinach leaves. a procedure to obtain a concentrated suspension of salmonella from contaminated spinach leaves is described that is based on methods outlined in the u.s. fda bacteriological analytical manual for the detection of salmonella ...201728212322
scarless deletion of up to seven methyl-accepting chemotaxis genes with an optimized method highlights key function of chem in salmonella typhimurium.site-directed scarless mutagenesis is an essential tool of modern pathogenesis research. we describe an optimized two-step protocol for genome editing in salmonella enterica serovar typhimurium to enable multiple sequential mutagenesis steps in a single strain. the system is based on the λ red recombinase-catalyzed integration of a selectable antibiotics resistance marker followed by replacement of this cassette. markerless mutants are selected by expressing the meganuclease i-scei which induces ...201728212413
pathogen biofilm formation on cantaloupe surface and its impact on the antibacterial effect of lauroyl arginate ethyl.pathogen biofilm at fruit surface may pose a particular risk to food safety. in this study, the biofilms of listeria monocytogenes v7 and salmonella enterica serovar typhimurium atcc 13311 on cantaloupe fruit surface were visualized, and the resistance of biofilms against lauroyl arginate ethyl (lae, an antibacterial compound) was evaluated. each bacterium was inoculated on isolated cantaloupe rind surfaces at 10(5)-10(6) cfu/cm(2) and after incubation for 2, 12, 24, and 48 h, the surfaces were ...201728213018
inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in ready-to-bake cookie dough by gamma and electron beam irradiation.this study was conducted to investigate the efficacy of gamma and electron beam irradiation to inactivate foodborne pathogens in ready-to-bake cookie dough and to determine the effect on quality by measuring color and texture changes. cookie dough inoculated with escherichia coli o157:h7, salmonella typhimurium, or listeria monocytogenes was subjected to gamma and electron beam irradiation, with doses ranging from 0 to 3 kgy. as the radiation dose increased, the inactivation effect increased amo ...201728213023
fusion of flagellin 2 with bivalent white spot syndrome virus vaccine increases survival in freshwater shrimp.despite large economic losses attributable to white spot syndrome virus (wssv), an infectious pathogen of penaeid shrimp and other crustaceans worldwide, no efficient vaccines or antiviral agents to control the virus are available at present. here, we designed and constructed baculovirus-based vaccines delivering genes encoding the wssv envelope proteins, vp28 and vp19. to enhance the immunogenicity of the baculovirus-based vaccine, we fused a salmonella typhimurium flagellin 2 (fl2) gene with v ...201728216094
fate of pathogenic bacteria associated with production of pickled sausage by using a cold fill process.preservation by pickling has been used for many years to extend the shelf life of various types of food products. by storing meat products in a brine solution containing an organic acid, salt, spices, as well as other preservatives, the ph of the product is reduced, thus increasing the safety and shelf life of the product. pickling may involve the use of heated brines to further add to the safety of the food product. when precooked, ready-to-eat (rte) sausages are pickled with a heated brine sol ...201628221846
neural network model for thermal inactivation of salmonella typhimurium to elimination in ground chicken: acquisition of data by whole sample enrichment, miniature most-probable-number method.predictive models are valuable tools for assessing food safety. existing thermal inactivation models for salmonella and ground chicken do not provide predictions above 71°c, which is below the recommended final cooked temperature of 73.9°c for chicken. they also do not predict when all salmonella are eliminated without extrapolating beyond the data used to develop them. thus, a study was undertaken to develop a model for thermal inactivation of salmonella to elimination in ground chicken at temp ...201728221884
inactivation of gram-positive bacteria by novel phenolic branched-chain fatty acids.novel phenolic branched-chain fatty acids (pbc-fas) were evaluated for their antimicrobial properties against both gram-positive ( listeria innocua , bacillus subtilis , enterococcus faecium ) and gram-negative ( escherichia coli , salmonella typhimurium, and pseudomonas tolaasii ) bacteria. in addition, pbc-fa derivatives, such as pbc-fa methyl ester mixture, methyl-branched fatty acid mixtures, and trimethylsilyl-pbc-fa methyl esters, were synthesized to study the structure activity relationsh ...201728221889
reduction of bacterial pathogens and potential surrogates on the surface of almonds using high-intensity 405-nanometer light.the disinfecting properties of high-intensity monochromatic blue light (mbl) were investigated against escherichia coli o157:h7, salmonella , and nonpathogenic bacteria inoculated onto the surface of almonds. mbl was generated from an array of narrow-band 405-nm light-emitting diodes. almonds were inoculated with higher or lower levels (8 or 5 cfu/g) of pathogenic e. coli o157:h7 and salmonella , as well as nonpathogenic e. coli k-12 and an avirulent strain of salmonella typhimurium, for evaluat ...201628221904
effect of temperature and nutrient concentration on survival of foodborne pathogens in deciduous fruit processing environments for effective hygiene management.temperature and good sanitation practices are important factors for controlling growth of microorganisms. fresh produce is stored at various temperatures to ensure quality and to prolong shelf life. when foodborne pathogens survive and grow on fresh produce at storage temperatures, then additional control strategies are needed to inactivate these pathogens. the aim of this study was to determine how temperatures associated with deciduous fruit processing and storage facilities (0.5, 4, and 21°c) ...201628221909
characterization and antimicrobial resistance of salmonella typhimurium isolates from clinically diseased pigs in korea.this study investigated the prevalence of salmonella enterica serovar and antimicrobial resistance in salmonella typhimurium isolates from clinically diseased pigs collected from 2008 to 2014 in korea. isolates were also characterized according to the presence of antimicrobial resistance genes and pulsed-field gel electrophoresis patterns. among 94 salmonella isolates, 81 (86.2%) were identified as being of the salmonella typhimurium serotype, followed by salmonella derby (6 of 94, 6.4%), salmon ...201628221916
effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste using 915-megahertz microwave and ohmic heating.the effect of power levels on inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes in tomato paste was investigated using 915-mhz microwave heating (mw) and ohmic heating (oh). heating uniformity, pathogen inactivation, and quality aspects were determined with 1.8-, 2.1-, 2.4-, and 3.0-kw mw and corresponding oh. ginafit was used to analyze pathogen inactivation. the heating uniformity of mw-treated samples was inferior to that of oh-treated samples at low ...201628221946
isolation, identification, and characterization of foodborne pathogens isolated from egg internal contents in china.eggs continue to be significant in terms of the world economy and human nutrition. the aim of this study was to estimate the prevalence of foodborne pathogens in a cross section of egg production types in china and to examine the virulence features of the isolated pathogens. three hundred eggs from three provinces were tested for salmonella , escherichia coli , staphylococcus aureus , and campylobacter . two eggs were positive for salmonella , one was positive for both e. coli and s. aureus , an ...201628221968
a bioprocessed polysaccharide from lentinus edodes mycelia cultures with turmeric protects chicks from a lethal challenge of salmonella gallinarum.our previous studies demonstrated that a bioprocessed polysaccharide (bpp) isolated from lentinus edodes mushroom mycelia cultures supplemented with black rice bran can protect mice against salmonella lipopolysaccharide-induced endotoxemia and reduce the mortality from salmonella typhimurium infection through upregulated t-helper 1 immunity. here, we report that a bpp from l. edodes mushroom mycelia liquid cultures supplemented with turmeric (referred to as bpp-turmeric) alters chicken macrophag ...201728221973
evaluation of a multiplex pcr assay for the identification of salmonella serovars enteritidis and typhimurium using retail and abattoir samples.a multiplex pcr was developed to identify the two most common serovars of salmonella causing foodborne illness in canada, namely, serovars enteritidis and typhimurium. the pcr was designed to amplify dna fragments from four salmonella genes, namely, inva gene (211-bp fragment), irob gene (309-bp fragment), typhimurium stm 4497 (523-bp fragment), and enteritidis se147228 (612-bp fragment). in addition, a 1,026-bp ribosomal dna (rdna) fragment universally present in bacterial species was included ...201728221989
alpk1 controls tifa/traf6-dependent innate immunity against heptose-1,7-bisphosphate of gram-negative bacteria.during infection by invasive bacteria, epithelial cells contribute to innate immunity via the local secretion of inflammatory cytokines. these are directly produced by infected cells or by uninfected bystanders via connexin-dependent cell-cell communication. however, the cellular pathways underlying this process remain largely unknown. here we perform a genome-wide rna interference screen and identify tifa and traf6 as central players of shigella flexneri and salmonella typhimurium-induced inter ...201728222186
selection of lactic acid bacteria with probiotic potential isolated from the fermentation process of "cupuaçu" (theobroma grandiflorum).in the present study, nine lactic acid bacteria isolated from the fermentation process of "cupuaçu" (theobroma grandiflorum) were selected for probiotic use. in vitro (resistance to gastrointestinal environment, in vitro antagonism and co-aggregation with pathogens) and in vivo (intestinal colonization and ex vivo antagonism in germ-free mice, cumulative mortality, translocation to liver and spleen, histopathological examination of liver and ileum and mrna cytokine gene expression during an expe ...201728224483
allograft inflammatory factor 1 is a regulator of transcytosis in m cells.m cells in follicle-associated epithelium (fae) are specialized antigen-sampling cells that take up intestinal luminal antigens. transcription factor spi-b regulates m-cell maturation, but the molecules that promote transcytosis within m cells are not fully identified. here we show that mouse allograft inflammatory factor 1 (aif1) is expressed by m cells and contributes to m-cell transcytosis. fae in aif1(-/-) mice has suppressed uptake of particles and commensal bacteria, compared with wild-typ ...201728224999
antibacterial and antioxidant activities of essential oils from artemisia herba-alba asso., pelargonium capitatum × radens and laurus nobilis l.essential oils are natural antimicrobials that have the potential to provide a safer alternative to synthetic antimicrobials currently used in the food industry. therefore, the aim of this study was to evaluate the antimicrobial and antioxidant activities of essential oils from white wormwood, rose-scented geranium and bay laurel against salmonella typhimurium and escherichia coli o157:h7 on fresh produce and to examine consumer acceptability of fresh produce treated with these essential oils. o ...201628231123
erratum for mccollister et al., whole-genome sequencing identifies in vivo acquisition of a blactx-m-27-carrying incfii transmissible plasmid as the cause of ceftriaxone treatment failure for an invasive salmonella enterica serovar typhimurium infection. 201728232307
detection of foodborne salmonella typhimurium outbreaks. 201728237433
efficient enrichment and analyses of bacteria at ultralow concentration with quick-response magnetic nanospheres.enrichment and purification of bacteria from complex matrices are crucial for their detection and investigation, in which magnetic separation techniques have recently show great application advantages. however, currently used magnetic particles all have their own limitations: magnetic microparticles exhibit poor binding capacity with targets, while magnetic nanoparticles suffer slow magnetic response and high loss rate during treatment process. herein, we used a highly controllable layer-by-laye ...201728241111
the antibacterial activity of extracts of nine plant species with good activity against escherichia coli against five other bacteria and cytotoxicity of extracts.the development of antibiotic resistant bacteria stems from a number of factors, including inappropriate use of antibiotics in human and animal health and their prolonged use as growth promoters at sub-clinical doses in poultry and livestock production. we were interested in investigating plants that could be useful in protecting humans or animals against diarrhoea. we decided to work on extracts of nine plant species with good activity against escherichia coli based on earlier work in the phyto ...201728241818
antimutagenic and anticlastogenic effects of turkish black tea on ta98 and ta100 strains of salmonella typhimurium (in vitro) and mice (in vivo).black tea has been reported to have significant antimutagenic and anticarcinogenic properties associated with its polyphenols theaflavins (tf) and thearubigins (tr). similarly, turkish black tea (tbt) also contains a considerable amount of tf and tr.201728245735
ecotoxicological survey of mnei and y65r-mnei proteins as new potential high-intensity sweeteners.low-calorie sweeteners are widespread. they are routinely introduced into commonly consumed food such as diet sodas, cereals, and sugar-free desserts. recent data revealed the presence in considerable quantities of some of these artificial sweeteners in water samples qualifying them as a class of potential new emerging contaminants. this study aimed at evaluating the ecotoxicity profile of mnei and y65r-mnei, two engineered products derived from the natural protein monellin, employing representa ...201728251536
prevalence, molecular and antimicrobial resistance of salmonella isolated from sausages in meknes, morocco.salmonella is among the most important food borne pathogens worldwide contaminating a wide range of animal products including meat products. the aims of this study go through two steps: the first step is to estimate the proportion of sausages products contaminated with salmonella in meknes city (morocco), which were collected from various shopping sites: butchery, street vendors, supermarket and souk (weekly market combines the population of the small villages around meknes city). the second one ...201728258000
antibacterial activity and synergistic antibacterial potential of biosynthesized silver nanoparticles against foodborne pathogenic bacteria along with its anticandidal and antioxidant effects.silver nanoparticles plays a vital role in the development of new antimicrobial substances against a number of pathogenic microorganisms. these nanoparticles due to their smaller size could be very effective as they can improve the antibacterial activity through lysis of bacterial cell wall. green synthesis of metal nanoparticles using various plants and plant products has recently been successfully accomplished. however, few studies have investigated the use of industrial waste materials in nan ...201728261161
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