Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| actin cross-linking domain of aeromonas hydrophila repeat in toxin a (rtxa) induces host cell rounding and apoptosis. | the repeat in toxin (rtx) of an environmental isolate atcc 7966 of aeromonas hydrophila consists of six genes (rtxachbde) organized in an operon similar to the gene organization found for the rtx of the vibrio species. the first gene in this operon (rtxa) encodes an exotoxin in vibrios, while other genes code for proteins needed for proper activation of rtxa and in secretion of this toxin from vibrio cholerae. however, the rtxa of atcc 7966, as well as from the clinical isolate ssu of a. hydroph ... | 2012 | 22814176 |
| identification and characterization of the functional toxboxes in the vibrio cholerae cholera toxin promoter. | following the consumption of contaminated food or water by a human host, the vibrio cholerae bacterium produces virulence factors, including cholera toxin (ct), which directly causes voluminous diarrhea, producing cholera. a complex regulatory network controls virulence gene expression and responds to various environmental signals and transcription factors. ultimately, toxt, a member of the arac/xyls transcription regulator family, is responsible for activating the transcription of the virulence ... | 2012 | 22821976 |
| antibacterial & antitoxic effects of the cardiovascular drug lacidipine in an animal model. | vibrio cholerae produces acute infection by liberating potent enterotoxin, called cholera toxin in human intestine. cardiovascular drug lacidipine possessing powerful in vitro action against v. cholerae was tested to determine its possible activity against a toxigenic v. cholerae strain in an established animal model. | 2012 | 22825612 |
| nontoxigenic vibrio cholerae septicemia in an immunocompromised patient. | we report a recent case of non-o1, non-o139, nontoxigenic vibrio cholerae septicemia in a post-liver-transplant immunocompromised patient associated with prior seafood consumption. non-o1, non-o139 v. cholerae strains have been reported in several cases of extraintestinal infections and seem to be emerging infectious agents especially in patients with immunocompromising conditions. | 2012 | 22830064 |
| mixing and matching siderophore clusters: structure and biosynthesis of serratiochelins from serratia sp. v4. | interrogation of the evolutionary history underlying the remarkable structures and biological activities of natural products has been complicated by not knowing the functions they have evolved to fulfill. siderophores-soluble, low molecular weight compounds-have an easily understood and measured function: acquiring iron from the environment. bacteria engage in a fierce competition to acquire iron, which rewards the production of siderophores that bind iron tightly and cannot be used or pirated b ... | 2012 | 22830960 |
| third-generation cephalosporin-resistant vibrio cholerae, india. | vibrio cholerae resistance to third-generation cephalosporins is rarely reported. we detected a strain that was negative for extended-spectrum β-lactamase and positive for the ampc disk test, modified hodge test, and edta disk synergy test and harbored the bladha-1 and blandm-1 genes. the antimicrobial drug susceptibility profile of v. cholerae should be monitored. | 2012 | 22840562 |
| prevalence and antibiotic resistance of bacterial pathogens isolated from childhood diarrhoea in four provinces of kenya. | diarrhoea is one of the main causes of morbidity and mortality among children in sub-saharan africa, and one of the main causes of hospital admissions in rural areas of kenya. in kenya, antimicrobial resistance surveillance has been conducted only at the institutional levels, with limited sharing of information and analysis of data. as a result, the actual scale of regional or national antimicrobial drug resistance is not well defined. | 2012 | 22842944 |
| antimicrobial resistance of vibrio cholerae o1 isolated during a cholera epidemic in 2011 in dry season in cote d'ivoire. | 2012 | 22842949 | |
| antibacterial activity of plasma from crocodile (crocodylus siamensis) against pathogenic bacteria. | the siamese crocodile (crocodylus siamensis) is a critically endangered species of freshwater crocodiles. crocodilians live with opportunistic bacterial infection but normally suffer no adverse effects. they are not totally immune to microbial infection, but their resistance thereto is remarkably effective. in this study, crude and purified plasma extracted from the siamese crocodile were examined for antibacterial activity against clinically isolated, human pathogenic bacterial strains and the ... | 2012 | 22846342 |
| identification of small molecules that antagonize diguanylate cyclase enzymes to inhibit biofilm formation. | bacterial biofilm formation is responsible for numerous chronic infections, causing a severe health burden. many of these infections cannot be resolved, as bacteria in biofilms are resistant to the host's immune defenses and antibiotic therapy. new strategies to treat biofilm-based infections are critically needed. cyclic di-gmp (c-di-gmp) is a widely conserved second-messenger signal essential for biofilm formation. as this signaling system is found only in bacteria, it is an attractive target ... | 2012 | 22850508 |
| holliday junction affinity of the base excision repair factor endo iii contributes to cholera toxin phage integration. | toxigenic conversion of vibrio cholerae bacteria results from the integration of a filamentous phage, ctx phage. integration is driven by the bacterial xer recombinases, which catalyse the exchange of a single pair of strands between the phage single-stranded dna and the host double-stranded dna genomes; replication is thought to convert the resulting pseudo-holliday junction (hj) intermediate into the final recombination product. the natural tendency of the xer recombinases to recycle hj interm ... | 2012 | 22863778 |
| antibiotic resistance of vibrio cholerae o1 el tor strains from the seventh pandemic in china, 1961-2010. | antibiotic resistance is observed with increasing frequency among epidemic vibrio cholerae strains in some countries. in this study, the antibiotic resistance profiles of v. cholerae o1 el tor strains isolated in china from 1961 to 2010 were analysed. the frequency of antibiotic resistance among the seventh pandemic el tor isolates from china remained low, except for resistance to nalidixic acid (45.9%), tetracycline (11%) and trimethoprim/sulfamethoxazole (38.5%). all test strains in the first ... | 2012 | 22867881 |
| x-ray structure of salmonella typhimurium uridine phosphorylase complexed with 5-fluorouracil and molecular modelling of the complex of 5-fluorouracil with uridine phosphorylase from vibrio cholerae. | uridine phosphorylase (uph), which is a key enzyme in the reutilization pathway of pyrimidine nucleoside metabolism, is a validated target for the treatment of infectious diseases and cancer. a detailed analysis of the interactions of uph with the therapeutic ligand 5-fluorouracil (5-fura) is important for the rational design of pharmacological inhibitors of these enzymes in prokaryotes and eukaryotes. expanding on the preliminary analysis of the spatial organization of the active centre of uph ... | 2012 | 22868762 |
| detection, isolation, and identification of vibrio cholerae from the environment. | recent molecular advances in microbiology have greatly improved the detection of bacterial pathogens in the environment. these improvements and a downward trend in the cost of molecular detection methods have contributed to increased frequency of detection of pathogenic microorganisms where traditional culture-based detection methods have failed. culture methods also have been greatly improved, and the confluence of the two suites of methods provides a powerful tool for detection, isolation, and ... | 2012 | 22875567 |
| multidrug-resistant vibrio cholerae o1 in belgaum, south india. | an outbreak of acute diarrhoea occurred in the belgundi area (population 3896) of belgaum taluka (population 815 581) in karnataka, south india, in june 2010. an estimated 16.22 % of people were affected and 0.16 % deaths were reported. vibrio cholerae o1 el tor was isolated from 18 of the 147 stool samples cultured. seven out of eight drinking water samples collected from different sources were found to be grossly contaminated with faecal coliforms. all isolates were multidrug resistant, with s ... | 2012 | 22878247 |
| arginine-specific mono adp-ribosylation in vitro of antimicrobial peptides by adp-ribosylating toxins. | among the several toxins used by pathogenic bacteria to target eukaryotic host cells, proteins that exert adp-ribosylation activity represent a large and studied family of dangerous and potentially lethal toxins. these proteins alter cell physiology catalyzing the transfer of the adp-ribose unit from nad to cellular proteins involved in key metabolic pathways. in the present study, we tested the capability of four of these toxins, to adp-ribosylate α- and β- defensins. cholera toxin (ct) from vi ... | 2012 | 22879887 |
| evaluation of tcpf-a2-ctb chimera and evidence of additive protective efficacy of immunizing with tcpf and ctb in the suckling mouse model of cholera. | the secreted colonization factor, tcpf, which is produced by vibrio cholerae 01 and 0139, has generated interest as a potential protective antigen in the development of a subunit vaccine against cholera. this study evaluated immunogenicity/protective efficacy of a tcpf holotoxin-like chimera (tcpf-a2-ctb) following intraperitoneal immunization compared to tcpf alone, a tcpf+ctb mixture, or ctb alone. immunization with the tcpf-a2-ctb chimera elicited significantly greater amounts of anti-tcpf ig ... | 2012 | 22879984 |
| vibrio cholerae/mimicus in fecal microbiota of healthy children in a cholera endemic urban slum setting in kolkata, india. | during a double-blind, randomized, placebo-controlled probiotic trial among 3758 children residing in an urban slum in kolkata, india, vibrio cholerae/mimicus was detected in fecal microbiota of healthy children. the importance of this finding in the local, regional and global transmission of cholera is discussed. | 2012 | 22882566 |
| antimicrobial activity of bioactive compounds and leaf extracts in jatropha tanjorensis. | jatropha tanjorensis was investigated scientifically to generate evidence for the efficacies reported in traditional systems and the results are given here. | 2012 | 22884742 |
| anti-diarrheal activity of (-)-epicatechin from chiranthodendron pentadactylon larreat: experimental and computational studies. | chiranthodendron pentadactylon larreat is frequently used in mexican traditional medicine as well as in guatemalan for several medicinal purposes, including their use in the control of diarrhea. | 2012 | 22884870 |
| presence of ctx gene cluster in environmental non-o1/o139 vibrio cholerae and its potential clinical significance. | the aim of this study was to understand the epidemiological linkage of clinical and environmental isolates of vibrio cholerae and to determine their genotypes and virulence genes content. | 2012 | 22885193 |
| rapid and sensitive quantification of vibrio cholerae and vibrio mimicus cells in water samples by use of catalyzed reporter deposition fluorescence in situ hybridization combined with solid-phase cytometry. | a new protocol for rapid, specific, and sensitive cell-based quantification of vibrio cholerae/vibrio mimicus in water samples was developed. the protocol is based on catalyzed reporter deposition fluorescence in situ hybridization (card-fish) in combination with solid-phase cytometry. for pure cultures, we were able to quantify down to 6 v. cholerae cells on one membrane with a relative precision of 39% and down to 12 cells with a relative precision of 17% after hybridization with the horseradi ... | 2012 | 22885749 |
| crystal structure of the vgrg1 actin cross-linking domain of the vibrio cholerae type vi secretion system. | vibrio cholerae is the cause of the diarrheal disease cholera. v. cholerae produces rtxa, a large toxin of the martx family, which is targeted to the host cell cytosol, where its actin cross-linking domain (acd) cross-links g-actin, leading to f-actin depolymerization, cytoskeleton rearrangements, and cell rounding. these effects on the cytoskeleton prevent phagocytosis and bacterial engulfment by macrophages, thus preventing v. cholerae clearance from the gut. the v. cholerae type vi secretion- ... | 2012 | 22898822 |
| refolding and functional assembly of the vibrio cholerae porin ompu recombinantly expressed in the cytoplasm of escherichia coli. | ompu is one of the major outer membrane porins of vibrio cholerae. ompu has been biochemically characterized previously for its 'porin'-property. however, previous studies have used the ompu protein extracted from the bacterial outer membrane envelope fractions. such method of isolation imposes limitations on the availability of the protein reagent, and also enhances the possibility of the ompu preparation being contaminated with lipid molecules of bacterial outer membrane origin, especially lip ... | 2012 | 22902612 |
| functional characterization of the stringent response regulatory gene dksa of vibrio cholerae and its role in modulation of virulence phenotypes. | in bacteria, nutrient deprivation evokes the stringent response, which is mediated by the small intracellular signaling molecule ppgpp. in gram negatives, the rela enzyme synthesizes and spot hydrolyzes ppgpp, although the latter protein also has weak synthetase activity. dksa, a recently identified rna polymerase binding transcription factor, acts as a coregulator along with ppgpp for controlling the stringent response. recently, we have shown that three genes, rela, spot, and relv, govern cell ... | 2012 | 22904284 |
| studies of dynamic protein-protein interactions in bacteria using renilla luciferase complementation are undermined by nonspecific enzyme inhibition. | the luciferase protein fragment complementation assay is a powerful tool for studying protein-protein interactions. two inactive fragments of luciferase are genetically fused to interacting proteins, and when these two proteins interact, the luciferase fragments can reversibly associate and reconstitute enzyme activity. though this technology has been used extensively in live eukaryotic cells, split luciferase complementation has not yet been applied to studies of dynamic protein-protein interac ... | 2012 | 22905225 |
| the archetype pseudomonas aeruginosa proteins tssb and tagj form a novel subcomplex in the bacterial type vi secretion system. | in pseudomonas aeruginosa three type vi secretion systems (t6sss) coexist, called h1- to h3-t6sss. several t6ss components are proposed to be part of a macromolecular complex resembling the bacteriophage tail. the t6ss protein hsie1 (tagj) is unique to the h1-t6ss and absent from the h2- and h3-t6sss. we demonstrate that hsie1 interacts with a predicted n-terminal α-helix in hsib1 (tssb) thus forming a novel subcomplex of the t6ss. hsib1 is homologous to the vibrio cholerae vipa component, which ... | 2012 | 22906320 |
| functional importance of a pair of conserved glutamic acid residues and of ca(2+) binding in the cbb(3)-type oxygen reductases from rhodobacter sphaeroides and vibrio cholerae. | the cbb(3)-type cytochrome c oxidases are members of the family of heme-copper proton pumping respiratory oxygen reductases. the structure of the cbb(3)-type oxidase from pseudomonas stutzeri reveals that, in addition to the six redox-active metal centers (two b-type hemes, three c-type hemes, and cu(b)), the enzyme also contains at least one ca(2+). the calcium bridges two propionate carboxyls at the interface between the low-spin heme b and the active-site heme b(3) and, in addition, is ligate ... | 2012 | 22913716 |
| [progress on xercd/dif site-specific recombination]. | in escherichia coli, 10% to 15% of growing bacteria produce chromosome dimers during dna replication. these dimers are resolved by xerc and xerd, two chromosome recombinases that target the dif sequence in the replication terminus of chromosome. phage ctxφ integrates into vibrio cholerae chromosome in a site-specific manner. however, ctxφ genome does not encode any recombinase, while recombinase xerc and xerd, which is coded by vibrio cholerae chromosome are required for the integration of ctxφ i ... | 2012 | 22917905 |
| emerging trends in the epidemiology of human astrovirus infection among infants, children and adults hospitalized with acute watery diarrhea in kolkata, india. | human astroviruses (hastvs) have now emerged as another common cause of non-bacterial acute gastroenteritis (age) in humans worldwide. this study investigated the epidemiology and genetic diversity of human astrovirus strains circulating among infants, younger children (up to 6 years), older children and adolescents (>6-17 years) and adults (18 years and above) hospitalized for diarrhea and their role in age in kolkata, india. a total of 2535 fecal samples were screened for the presence of known ... | 2012 | 22921731 |
| structural and functional importance of outer membrane proteins in vibrio cholerae flagellum. | vibrio cholerae has a sheath-covered monotrichous flagellum that is known to contribute to virulence. although the structural organization of the v. cholerae flagellum has been extensively studied, the involvement of outer membrane proteins as integral components in the flagellum still remains elusive. here we show that flagella produced by v. cholerae o1 el tor strain c6706 were two times thicker than those from two other gram-negative bacteria. a c6706 mutant strain (ssy11) devoid of two outer ... | 2012 | 22923112 |
| sialidase, chondroitinase abc, and combination therapy after spinal cord contusion injury. | axon regeneration in the central nervous system is severely hampered, limiting functional recovery. this is in part because of endogenous axon regeneration inhibitors that accumulate at the injury site. therapeutic targeting of these inhibitors and their receptors may facilitate axon outgrowth and enhance recovery. a rat model of spinal cord contusion injury was used to test the effects of two bacterial enzyme therapies that target independent axon regeneration inhibitors, sialidase (vibrio chol ... | 2013 | 22934782 |
| single point mutation in vibrio cholerae cytolysin compromises the membrane pore-formation mechanism of the toxin. | vibrio cholerae cytolysin (vcc) belongs to the family of β-barrel pore-forming protein toxins. vcc is secreted by the bacteria as water-soluble monomers, which upon binding to target eukaryotic cells form transmembrane heptameric β-barrel channels. high-resolution 3d structures are described both for the water-soluble monomeric form and the transmembrane oligomeric pore; albeit that our understanding of the mechanistic details of the membrane pore-formation process remains incomplete. here, we r ... | 2012 | 22934938 |
| cholera epidemiology in nigeria: an overview. | cholera is an acute diarrhoeal infection caused by ingestion of food or water contaminated with the bacterium, vibrio cholera. choleragenic v. cholera o1 and o139 are the only causative agents of the disease. the two most distinguishing epidemiologic features of the disease are its tendency to appear in explosive outbreaks and its predisposition to causing pandemics that may progressively affect many countries and spread into continents. despite efforts to control cholera, the disease continues ... | 2012 | 22937199 |
| [characteristics of vibrio cholerae cef (cho cell elongating factor): bioinformatics analysis and experimental data]. | bioinformatics analysis of the primary and secondary structure of the vibrio cholerae cef (cho cell elongating factor) protein was conducted. similarity with triacylglycerol lipases and cytotonic toxins of other bacterial species was observed. cef was predicted to be a heat-tolerant serine lipase with the kunitz domain and leucine zipper. these data were confirmed experimentally. the cefs of the two biotypes of v. cholerae o1, as well as o139 and nono1/nono139 serogroups, were purified from the ... | 2012 | 22937564 |
| [molecular-genetic analysis of the epidemical strains of the vibrio cholerae el tor isolated from the siberian and maritime regions of russia]. | the detection of the biotype-specificity, pathogenicity determinants, and sequencing of the ctxb gene and the ctxab promoter was carried out for analysis of the vibrio cholerae el tor strains genome structure. the strains (n = 90) were isolated during cholera epidemic outbreaks in siberia and the far east. all toxigenic vibrio cholerae el tor strains were divided into two groups: the first group included strains isolated during 1970s: they had the genotype ctxb3+rstrel+rstrcl-rstc+tlc+tbr4. all ... | 2012 | 22937565 |
| crystal structures of a ctxphi piii domain unbound and in complex with a vibrio cholerae tola domain reveal novel interaction interfaces. | vibrio cholerae colonize the small intestine where they secrete cholera toxin, an adp-ribosylating enzyme that is responsible for the voluminous diarrhea characteristic of cholera disease. the genes encoding cholera toxin are located on the genome of the filamentous bacteriophage, ctxϕ, that integrates as a prophage into the v. cholerae chromosome. ctxϕ infection of v. cholerae requires the toxin-coregulated pilus and the periplasmic protein tola. this infection process parallels that of escheri ... | 2012 | 22942280 |
| immune-stimulating potential of cell envelope proteins from vibrio cholerae associated to chitosan microparticles: an in vitro study. | cholera is a severe diarrheal disease that remains an important cause of illness and death in many parts of the world. | 2012 | 22947068 |
| investigation of real-time photorepair activity on dna via surface plasmon resonance. | the cyclobutane pyrimidine dimer (cpd) and 6-4 lesion formations along with the specific breaks on strands are the most common type of dna damage caused by ultraviolet light (uv) irradiation. cpd photolyase i and ii construct two subfamilies of flavoproteins, which have recognition and repair capabilities of cpd sites on both single stranded (ssdna) and double stranded dna (dsdna) with the aid of blue light energy. the other types of flavoprotein family consist of cryptochromes (cry) that act as ... | 2012 | 22952969 |
| cholera outbreak in senegal in 2005: was climate a factor? | cholera is an acute diarrheal illness caused by vibrio cholerae and occurs as widespread epidemics in africa. in 2005, there were 31,719 cholera cases, with 458 deaths in the republic of senegal. we retrospectively investigated the climate origin of the devastating floods in mid-august 2005, in the dakar region of senegal and the subsequent outbreak of cholera along with the pattern of cholera outbreaks in three other regions of that country. we compared rainfall patterns between 2002 and 2005 a ... | 2012 | 22952995 |
| clonal transmission, dual peak, and off-season cholera in bangladesh. | vibrio cholerae is an estuarine bacterium associated with a single peak of cholera (march-may) in coastal villages of bangladesh. for an unknown reason, however, cholera occurs in a unique dual peak (march-may and september-november) pattern in the city of dhaka that is bordered by a heavily polluted freshwater river system and flood embankment. in august 2007, extreme flooding was accompanied by an unusually severe diarrhea outbreak in dhaka that resulted in a record high illness. this study wa ... | 2011 | 22957115 |
| antimicrobial activity of the crude extract of piper sarmentosum against methicilin-resistant staphylococcus aureus (mrsa), escherichia coli, vibrio cholera and streptococcus pneumoniae. | the emergence of novel diseases caused by microbial pathogens and the undesirable side effects of certain antibiotics has been a recent dilemma in the medical arena. consequently, it has stirred the discovery of many naturally occurring agents which could possibly provide important ramifications against various pharmacological targets and to combat various ailments. the main aim of the present study was to determine the antimicrobial activity of the crude methanolic extract of piper (p.) sarment ... | 2012 | 22957424 |
| high depth, whole-genome sequencing of cholera isolates from haiti and the dominican republic. | whole-genome sequencing is an important tool for understanding microbial evolution and identifying the emergence of functionally important variants over the course of epidemics. in october 2010, a severe cholera epidemic began in haiti, with additional cases identified in the neighboring dominican republic. we used whole-genome approaches to sequence four vibrio cholerae isolates from haiti and the dominican republic and three additional v. cholerae isolates to a high depth of coverage (>2000x); ... | 2012 | 22963323 |
| frequency of reexposure to vibrio cholerae o1 evaluated by subsequent vibriocidal titer rise after an episode of severe cholera in a highly endemic area in bangladesh. | vibriocidal antibody is a marker of recent exposure to vibrio cholerae o1 infection. we examined vibriocidal titers for 1 year after an episode of severe cholera in patients in dhaka, bangladesh; 16 of 53 (30%) patients had a fourfold or greater increase in vibriocidal titer between 6 and 12 months after an episode of severe cholera, suggesting reexposure to the organism. among patients with rises in titers during follow-up, the patients initially infected with serotype ogawa had earlier rises i ... | 2012 | 22964723 |
| screening helicobacter pylori genes induced during infection of mouse stomachs. | to investigate the effect of in vivo environment on gene expression in helicobacter pylori (h. pylori) as it relates to its survival in the host. | 2012 | 22969195 |
| microbial diversity and potential pathogens in ornamental fish aquarium water. | ornamental fishes are among the most popular and fastest growing categories of pets in the united states (u.s.). the global scope and scale of the ornamental fish trade and growing popularity of pet fish in the u.s. are strong indicators of the myriad economic and social benefits the pet industry provides. relatively little is known about the microbial communities associated with these ornamental fishes or the aquarium water in which they are transported and housed. using conventional molecular ... | 2012 | 22970112 |
| integrating conjugative elements of the sxt/r391 family from fish-isolated vibrios encode restriction-modification systems that confer resistance to bacteriophages. | integrating conjugative elements (ices) of the sxt/r391 family have been described in vibrios, mainly vibrio cholerae, and other bacteria as carriers of variable gene content conferring adaptive advantages upon their hosts, including antimicrobial resistance and motility regulation. however, our knowledge on their host range and ecological significance is still limited. here, we report the identification and characterization of icevsppor3 and icevalspa1, two novel ices of the sxt/r391 family fro ... | 2013 | 22974320 |
| genetic characteristics of drug-resistant vibrio cholerae o1 causing endemic cholera in dhaka, 2006-2011. | vibrio cholerae o1 biotype el tor (et), causing the seventh cholera pandemic, was recently replaced in bangladesh by an altered et possessing ctxb of the classical (cl) biotype, which caused the first six cholera pandemics. in the present study, v. cholerae o1 strains associated with endemic cholera in dhaka between 2006 and 2011 were analysed for major phenotypic and genetic characteristics. of 54 representative v. cholerae isolates tested, all were phenotypically et and showed uniform resistan ... | 2012 | 22977073 |
| [analysis of monitoring results for vibrio cholerae in wuzhou city from 2009 to 2010]. | 2011 | 22991708 | |
| the 1.2 å resolution crystal structure of tcpg, the vibrio cholerae dsba disulfide-forming protein required for pilus and cholera-toxin production. | the enzyme tcpg is a periplasmic protein produced by the gram-negative pathogen vibrio cholerae. tcpg is essential for the production of toxr-regulated proteins, including virulence-factor pilus proteins and cholera toxin, and is therefore a target for the development of a new class of anti-virulence drugs. here, the 1.2 å resolution crystal structure of tcpg is reported using a cryocooled crystal. this structure is compared with a previous crystal structure determined at 2.1 å resolution from d ... | 2012 | 22993083 |
| structural insight into the isc domain of vibb from vibrio cholerae at atomic resolution: a snapshot just before the enzymatic reaction. | the n-terminal isochorismatase (isc) domain of vibb (vibb-isc) catalyzes the vinyl ether hydrolysis of isochorismate to 2,3-dihydro-2,3-dihydroxybenzoate and pyruvate. structures of the isc domain and its complex with isochorismate have been determined at 1.35 and 1.10 å resolution, respectively. two catalytic waters which were absent from previously reported homologous structures were observed adjacent to isochorismate and the catalytic residues (asp35 and lys118) in the vibb-isc complex. molec ... | 2012 | 22993087 |
| structural characterization and comparison of three acyl-carrier-protein synthases from pathogenic bacteria. | some bacterial type ii fatty-acid synthesis (fas ii) enzymes have been shown to be important candidates for drug discovery. the scientific and medical quest for new fas ii protein targets continues to stimulate research in this field. one of the possible additional candidates is the acyl-carrier-protein synthase (acps) enzyme. its holo form post-translationally modifies the apo form of an acyl carrier protein (acp), which assures the constant delivery of thioester intermediates to the discrete e ... | 2012 | 22993090 |
| structure of the cfa/iii major pilin subunit cofa from human enterotoxigenic escherichia coli determined at 0.90 å resolution by sulfur-sad phasing. | cofa, a major pilin subunit of colonization factor antigen iii (cfa/iii), forms pili that mediate small-intestinal colonization by enterotoxigenic escherichia coli (etec). in this study, the crystal structure of an n-terminally truncated version of cofa was determined by single-wavelength anomalous diffraction (sad) phasing using five sulfurs in the protein. given the counterbalance between anomalous signal strength and the undesired x-ray absorption of the solvent, diffraction data were collect ... | 2012 | 22993096 |
| comparison of immune responses to the o-specific polysaccharide and lipopolysaccharide of vibrio cholerae o1 in bangladeshi adult patients with cholera. | immunity against vibrio cholerae o1 is serogroup specific, and serogrouping is defined by the o-specific polysaccharide (osp) part of lipopolysaccharide (lps). despite this, human immune responses to v. cholerae osp have not previously been characterized. we assessed immune responses against v. cholerae osp in adults with cholera caused by v. cholerae o1 el tor serotype inaba or ogawa in dhaka, bangladesh, using o1 osp-core-bovine serum albumin (ospc:bsa) conjugates; responses targeted osp in th ... | 2012 | 22993410 |
| the occurrence of pathogenic bacteria in some ships' ballast water incoming from various marine regions to the sea of marmara, turkey. | the composition and frequency of antibiotic resistance of pathogenic bacteria, the abundance of heterotrophic aerobic bacteria (hpc) and possible in-situ use of chromogenic agar were investigated in the ships' ballast water coming from different regions of the world to the sea of marmara, turkey for the first time. the samples that were taken from 21 unit ships coming from various marine environments of the southern china sea, the atlantic ocean, the mediterranean and the black sea to the sea of ... | 2012 | 22998778 |
| crystal structure of hutz, a heme storage protein from vibrio cholerae: a structural mismatch observed in the region of high sequence conservation. | hutz is the sole heme storage protein identified in the pathogenic bacterium vibrio cholerae and is required for optimal heme utilization. however, no heme oxygenase activity has been observed with this protein. thus far, hutz's structure and heme-binding mechanism are unknown. | 2012 | 23013214 |
| metr-regulated vibrio cholerae metabolism is required for virulence. | lysr-type transcriptional regulators (lttrs) are the largest, most diverse family of prokaryotic transcription factors, with regulatory roles spanning metabolism, cell growth and division, and pathogenesis. using a sequence-defined transposon mutant library, we screened a panel of v. cholerae el tor mutants to identify lttrs required for host intestinal colonization. surprisingly, out of 38 lttrs, only one severely affected intestinal colonization in the suckling mouse model of cholera: the meth ... | 2012 | 23015737 |
| natural competence in vibrio cholerae is controlled by a nucleoside scavenging response that requires cytr-dependent anti-activation. | competence for genetic transformation in vibrio cholerae is triggered by chitin-induced transcription factor tfox and quorum sensing (qs) regulator hapr. transformation requires expression of comea, described as a dna receptor in other competent bacteria. a screen for mutants that poorly expressed a comea-luciferase fusion identified cytr, encoding the nucleoside scavenging cytidine repressor, previously shown in v. cholerae to be a biofilm repressor and positively regulated by tfox, but not lin ... | 2012 | 23016895 |
| preventing maritime transfer of toxigenic vibrio cholerae. | organisms, including vibrio cholerae, can be transferred between harbors in the ballast water of ships. zones in the caribbean region where distance from shore and water depth meet international maritime organization guidelines for ballast water exchange are extremely limited. use of ballast water treatment systems could mitigate the risk for organism transfer. | 2012 | 23017338 |
| activation of cholera toxin production by anaerobic respiration of trimethylamine n-oxide in vibrio cholerae. | vibrio cholerae is a gram-negative bacterium that causes cholera. although the pathogenesis caused by this deadly pathogen takes place in the intestine, commonly thought to be anaerobic, anaerobiosis-induced virulence regulations are not fully elucidated. anerobic growth of the v. cholerae strain, n16961, was promoted when trimethylamine n-oxide (tmao) was used as an alternative electron acceptor. strikingly, cholera toxin (ct) production was markedly induced during anaerobic tmao respiration. n ... | 2012 | 23019319 |
| establishment and characterization of an epithelial cell line from thymus of catla catla (hamilton, 1822). | a cell line, cte, derived from catla (catla catla) thymus has been established by explant method and subcultured for more than 70 passages over a period of 400 days. the cell line has been maintained in l-15 (leibovitz) medium supplemented with 10% fetal bovine serum. cte cell line consists of homogeneous population of epithelial-like cells and grows optimally at 28°c. karyotype analysis revealed that the modal chromosome number of cte cells was 50. partial amplification, sequencing and alignmen ... | 2013 | 23026220 |
| cloning, purification, crystallization and preliminary x-ray analysis of two low-molecular-weight protein tyrosine phosphatases from vibrio cholerae. | low-molecular-weight protein tyrosine phosphatases (lmwptps) are small cytoplasmic enzymes of molecular weight ∼18 kda that belong to the large family of protein tyrosine phosphatases (ptps). despite their wide distribution in both prokaryotes and eukaryotes, their exact biological role in bacterial systems is not yet clear. two low-molecular-weight protein tyrosine phosphatases (vclmwptp-1 and vclmwptp-2) from the gram-negative bacterium vibrio cholerae have been cloned, overexpressed, purified ... | 2012 | 23027748 |
| phase variable o antigen biosynthetic genes control expression of the major protective antigen and bacteriophage receptor in vibrio cholerae o1. | the vibrio cholerae lipopolysaccharide o1 antigen is a major target of bacteriophages and the human immune system and is of critical importance for vaccine design. we used an o1-specific lytic bacteriophage as a tool to probe the capacity of v. cholerae to alter its o1 antigen and identified a novel mechanism by which this organism can modulate o antigen expression and exhibit intra-strain heterogeneity. we identified two phase variable genes required for o1 antigen biosynthesis, mana and wbel. ... | 2012 | 23028317 |
| survival of vibrio cholerae in nutrient-poor environments is associated with a novel "persister" phenotype. | in response to antibiotic and/or environmental stress, some species of bacteria shift to a "persister" phenotype. although toxigenic vibrio cholerae, responsible for the disease cholera, can be found in nutrient-poor aquatic environments in endemic areas, the underlying mechanism(s) by which culturable cells persist in these environmental reservoirs is largely unknown. here we report that introduction of v. cholerae into a nutrient-poor filter sterilized lake water (fslw) microcosm promoted a sh ... | 2012 | 23028836 |
| a study of antimicrobial activity, acute toxicity and cytoprotective effect of a polyherbal extract in a rat ethanol-hcl gastric ulcer model. | the decoction of the aerial parts of rhynchosia recinosa (a.rich.) bak. [fabaceae] is used in combination with the stem barks of ozoroa insignis del. (anacardiaceae), maytenus senegalensis (lam.) excell. [celastraceae] entada abyssinica steud. ex a.rich [fabaceae] and lannea schimperi (hochst.)engl. [anacardiaceae] as a traditional remedy for managing peptic ulcers. however, the safety and efficacy of this polyherbal preparation has not been evaluated. this study reports on the phytochemical pro ... | 2012 | 23031266 |
| distribution of potentially pathogenic enteric bacteria in coastal sea waters along the southern kerala coast, india. | this study evaluated the relationship between the traditional indicators of faecal pollution, total coliforms (tc), faecal coliforms (fc) and faecalstreptococci (fs), and the presence of few potentially pathogenic enteric bacteria, vibrio cholerae (vc), vibrio parahaemolyticus (vp), shigella spp. (sh) and salmonella spp. (sl) in coastal sea water. the distributional statuses of these bacteria were also studied along the southern kerala coast. cluster analyses were done to identify similar groups ... | 2012 | 23033645 |
| non-o1 vibrio cholerae unlinked to cholera in haiti. | 2012 | 23035253 | |
| risk reduction assessment of waterborne salmonella and vibrio by a chlorine contact disinfectant point-of-use device. | unsafe drinking water continues to burden developing countries despite improvements in clean water delivery and sanitation, in response to millennium development goal 7. salmonella serotype typhi and vibrio cholerae bacteria can contaminate drinking water, causing waterborne typhoid fever and cholera, respectively. household water treatment (hwt) systems are widely promoted to consumers in developing countries but it is difficult to establish their benefits to the population for specific disease ... | 2013 | 23041054 |
| the vibrio cholerae trh gene is coordinately regulated in vitro with type iii secretion system genes by vttr(a)/vttr(b) but does not contribute to caco2-bbe cell cytotoxicity. | numerous virulence factors have been associated with pathogenic non-o1/non-o139 serogroup strains of vibrio cholerae. among them are the thermostable direct hemolysin (tdh) and the tdh-related hemolysin (trh), which share amino acid similarities to the tdh and trh proteins of vibrio parahaemolyticus, where they have been shown to contribute to pathogenesis. although tdh and trh homologs can be encoded on extrachromosomal elements in v. cholerae, type iii secretion system (t3ss)-positive strains, ... | 2012 | 23045478 |
| differential effects of intranasal vaccination with recombinant ncpdi in different mouse models of neospora caninum infection. | in this study, mice were vaccinated intranasally with recombinant n. caninum protein disulphide isomerase (ncpdi) emulsified in cholera toxin (ct) or cholera toxin subunit b (ctb) from vibrio cholerae. the effects of vaccination were assessed in the murine nonpregnant model and the foetal infection model, respectively. in the nonpregnant mice, previous results were confirmed, in that intranasal vaccination with recncpdi in ct was highly protective, and low cerebral parasite loads were noted upon ... | 2013 | 23046358 |
| dynamics of classical-el tor switch of vibrio cholerae strains isolated from 1961-2010. | 2012 | 23046757 | |
| assessing point-of-care device specifications and needs for pathogen detection in emergencies and disasters. | background: we assessed point-of-care device specifications and needs for pathogen detection in urgent care, emergencies, and disasters. methods: we surveyed american association for clinical chemistry members and compared responses to those of disaster experts. online surveymonkey questions covered performance characteristics, device design, pathogen targets, and other specifications. results: for disasters, respondents preferred direct sample collection with a disposable test cassette that sto ... | 2012 | 23049471 |
| re-emergence of cholera in the americas: risks, susceptibility, and ecology. | the re-emergence of cholera in haiti has established a new reservoir for the seventh cholera pandemic which threatens to spread to other countries in the americas. | 2012 | 23055647 |
| a bacterial hemerythrin domain regulates the activity of a vibrio cholerae diguanylate cyclase. | the first demonstrated example of a regulatory function for a bacterial hemerythrin (bhr) domain is reported. bhrs have a characteristic sequence motif providing ligand residues for a type of non-heme diiron site that is known to bind o(2) and undergo autoxidation. the amino acid sequence encoded by the vc1216 gene from vibrio cholerae o1 biovar el tor str. n16961 contains an n-terminal bhr domain connected to a c-terminal domain characteristic of bacterial diguanylate cyclases (dgcs) that catal ... | 2012 | 23057727 |
| ndm-1 polymicrobial infections including vibrio cholerae. | 2012 | 23063285 | |
| a multidomain hub anchors the chromosome segregation and chemotactic machinery to the bacterial pole. | the cell poles constitute key subcellular domains that are often critical for motility, chemotaxis, and chromosome segregation in rod-shaped bacteria. however, in nearly all rods, the processes that underlie the formation, recognition, and perpetuation of the polar domains are largely unknown. here, in vibrio cholerae, we identified hubp (hub of the pole), a polar transmembrane protein conserved in all vibrios, that anchors three para-like atpases to the cell poles and, through them, controls po ... | 2012 | 23070816 |
| review of two decades of cholera diagnostics--how far have we really come? | cholera, an ancient scourge, continues to inflict high rates of mortality today. the rising incidence of epidemics in areas of poor sanitation and crowding highlight the need for better epidemic prevention and early response. such interventions require the availability of rapid and accurate diagnostic techniques to trigger timely response and mitigate the scale of the outbreak. the current gold standard of bacterial culture is inadequate for rapid diagnosis, highlighting the overarching neglect ... | 2012 | 23071851 |
| analysis toward innovative herbal antibacterial and antifungal drugs. | the antimicrobial activities of four medicinal plants argemona mexicana, achyranthes aspera, catharanthus roseus, and syzygium cumini were evaluated against escherichia coli, vibrio cholerae, klebsiella pneumoniae, proteus vulgaris, bacillus subtilis, salmonella typhi and three aspergillus species. extracts from achyranthes aspera and catharanthus roseus showed the highest antimicrobial potential (mic 0.375-0.750 mg/ml) while extract from argemona mexicana and syzygium cumini, showed less activi ... | 2012 | 23072646 |
| phage-bacterial interactions in the evolution of toxigenic vibrio cholerae. | understanding the genetic and ecological factors which support the emergence of new clones of pathogenic bacteria is vital to develop preventive measures. vibrio cholerae the causative agent of cholera epidemics represents a paradigm for this process in that this organism evolved from environmental non-pathogenic strains by acquisition of virulence genes. the major virulence factors of v. cholerae, cholera toxin (ct) and toxin coregulated pilus (tcp) are encoded by a lysogenic bacteriophage (ctx ... | 2012 | 23076327 |
| cochleates derived from vibrio cholerae o1 proteoliposomes: the impact of structure transformation on mucosal immunisation. | cochleates are phospholipid-calcium precipitates derived from the interaction of anionic lipid vesicles with divalent cations. proteoliposomes from bacteria may also be used as a source of negatively charged components, to induce calcium-cochleate formation. in this study, proteoliposomes from v. cholerae o1 (plc) (sized 160.7±1.6 nm) were transformed into larger (16.3±4.6 µm) cochleate-like structures (named adjuvant finlay cochleate 2, afco2) and evaluated by electron microscopy (em). measurem ... | 2012 | 23077508 |
| diversity and antibiograms of bacterial organisms isolated from samples of household drinking-water consumed by hiv-positive individuals in rural settings, south africa. | diarrhoea is a hallmark of hiv infections in developing countries, and many diarrhoea-causing agents are often transmitted through water. the objective of the study was to determine the diversity and antibiotic susceptibility profiles of bacterial organisms isolated from samples of household drinking-water consumed by hiv-infected and aids patients. in the present study, household water stored for use by hiv-positive patients was tested for microbial quality, and isolated bacterial organisms wer ... | 2012 | 23082625 |
| investigation into immunological responses against a native recombinant ctb whole-cell vibrio cholerae vaccine in a rabbit model. | the aim of this study was to express and purify the recombinant ctb (rctb) protein from vibrio cholerae and investigate the biological and immunological characteristics of purified protein in rabbit animal model and in combination with iranian inactivated v. cholerae whole cells as a domestic recombinant wc-ctb vaccine. | 2013 | 23082752 |
| presence of sxt integrating conjugative element in marine bacteria isolated from the mucus of the coral fungia echinata from andaman sea. | in this study, we characterize 18 cultivable bacteria associated within the mucus of the coral fungia echinata from andaman sea, india. 16s rrna gene sequence analysis showed that all the 18 strains isolated in this study from the coral mucus belong to the group gammaproteobacteria and majority of them were identified as vibrio core group. our objective was to investigate the presence of the sxt/r391 integrating conjugative elements (ices) targeting integrase int(sxt) and sxt hotspot iv genetic ... | 2013 | 23083057 |
| role of the cai-1 fatty acid tail in the vibrio cholerae quorum sensing response. | quorum sensing is a mechanism of chemical communication among bacteria that enables collective behaviors. in v. cholerae, the etiological agent of the disease cholera, quorum sensing controls group behaviors including virulence factor production and biofilm formation. the major v. cholerae quorum-sensing system consists of the extracellular signal molecule called cai-1 and its cognate membrane bound receptor called cqss. here, the ligand binding activity of cqss is probed with structural analogu ... | 2012 | 23092313 |
| transflp--a method to genetically modify vibrio cholerae based on natural transformation and flp-recombination. | several methods are available to manipulate bacterial chromosomes(1-3). most of these protocols rely on the insertion of conditionally replicative plasmids (e.g. harboring pir-dependent or temperature-sensitive replicons(1,2)). these plasmids are integrated into bacterial chromosomes based on homology-mediated recombination. such insertional mutants are often directly used in experimental settings. alternatively, selection for plasmid excision followed by its loss can be performed, which for gra ... | 2012 | 23093249 |
| transcriptomic profiling of the oyster pathogen vibrio splendidus opens a window on the evolutionary dynamics of the small rna repertoire in the vibrio genus. | work in recent years has led to the recognition of the importance of small regulatory rnas (srnas) in bacterial regulation networks. new high-throughput sequencing technologies are paving the way to the exploration of an expanding srna world in nonmodel bacteria. in the vibrio genus, compared to the enterobacteriaceae, still a limited number of srnas have been characterized, mostly in vibrio cholerae, where they have been shown to be important for virulence, as well as in vibrio harveyi. in addi ... | 2012 | 23097430 |
| semi-nested polymerase chain reaction for detection of toxigenic vibrio cholerae from environmental water samples. | a rapid and sensitive direct cell semi-nested pcr assay was developed for the detection of viable toxigenic v. cholerae in environmental water samples. the semi-nested pcr assay amplified cholera toxin (ctxa2b) gene present in the toxigenic v. cholerae. the detection sensitivity of direct cell semi-nested pcr was 2 × 10(3) cfu of v. cholerae whereas direct cell single-step pcr could detect 2 × 10(4) cfu of v. cholerae. the performance of the assay was evaluated using environmental water samples ... | 2007 | 23100668 |
| detection of aerolysin gene in aeromonas hydrophila isolated from fish and pond water. | aerolysin is a hemolytic toxin encoded by aerolysin gene (1482 bp) that plays a key role in the pathogenesis of aeromonas hydrophila infection in fish. new speciesspecific primers were designed to amplify 326 bp conserved region of aerolysin gene for a. hydrophila. twenty-five isolates of a. hydrophila recovered from fish and pond water were studied for detection of aerolysin gene. aerolysin gene was detected in 85% of the isolates during the study. the designed primers were highly specific and ... | 2008 | 23100746 |
| antimicrobial susceptibility and molecular characterization of vibrio cholerae from cholera outbreaks in chennai. | the genotype and antibiotic resistance pattern of the toxigenic vibrio cholerae strains associated with cholera outbreaks vary frequently. fifty-one v. cholerae strains isolated from cholera outbreaks in chennai (2002-2005) were screened for the presence of virulence and regulatory genes by multiplex polymerase chain reaction (pcr) assay. genotyping of the isolates was done by vc1 primers derived from enterobacterial repetitive intergenic consensus (eric)-related sequence in v. cholerae. all the ... | 2009 | 23100755 |
| characterization of vibrio cholerae from deep ground water in a cholera endemic area in central india. | a total of 8 out of 11 deep ground water samples collected from different villages in central india were found contaminated with vibrio cholerae non o1, non o139. in a multiplex pcr, isolates were found positive for ompw gene but negative for ctxab and rfbo1 genes. however, isolates from two places were positive for tcp and zot genes, indicating their intestinal colonization and toxigenic potential. antibiotic susceptibility studies revealed that all isolates were multidrug resistant. although, ... | 2009 | 23100781 |
| quantitative microbial risk assessment of pathogenic vibrios in marine recreational waters of southern california. | this study investigated the occurrence of three types of vibrios in southern california recreational beach waters during the peak marine bathing season in 2007. over 160 water samples were concentrated and enriched for the detection of vibrios. four sets of pcr primers, specific for vibrio cholerae, v. parahaemolyticus, and v. vulnificus species and the v. parahaemolyticus toxin gene, respectively, were used for the amplification of bacterial genomic dna. of 66 samples from doheny state beach, c ... | 2013 | 23104412 |
| the transcriptional landscape of the deep-sea bacterium photobacterium profundum in both a toxr mutant and its parental strain. | the deep-sea bacterium photobacterium profundum is an established model for studying high pressure adaptation. in this paper we analyse the parental strain db110 and the toxr mutant tw30 by massively parallel cdna sequencing (rna-seq). toxr is a transmembrane dna-binding protein first discovered in vibrio cholerae, where it regulates a considerable number of genes involved in environmental adaptation and virulence. in p. profundum the abundance and activity of this protein is influenced by hydro ... | 2012 | 23107454 |
| constitutive type vi secretion system expression gives vibrio cholerae intra- and interspecific competitive advantages. | the type vi secretion system (t6ss) mediates protein translocation across the cell membrane of gram-negative bacteria, including vibrio cholerae - the causative agent of cholera. all v. cholerae strains examined to date harbor gene clusters encoding a t6ss. structural similarity and sequence homology between components of the t6ss and the t4 bacteriophage cell-puncturing device suggest that the t6ss functions as a contractile molecular syringe to inject effector molecules into prokaryotic and eu ... | 2012 | 23110230 |
| a single native ganglioside gm1-binding site is sufficient for cholera toxin to bind to cells and complete the intoxication pathway. | cholera toxin (ct) from vibrio cholerae is responsible for the majority of the symptoms of the diarrheal disease cholera. ct is a heterohexameric protein complex with a 240-residue a subunit and a pentameric b subunit of identical 103-residue b polypeptides. the a subunit is proteolytically cleaved within a disulfide-linked loop to generate the a1 and a2 fragments. the b subunit of wild-type (wt) ct binds 5 cell surface ganglioside gm(1) (gm(1)) molecules, and the toxin-gm(1) complex traffics fr ... | 2012 | 23111873 |
| study of avidity of antigen-specific antibody as a means of understanding development of long-term immunological memory after vibrio cholerae o1 infection. | the avidity of antibodies to specific antigens and the relationship of avidity to memory b cell responses to these antigens have not been studied in patients with cholera or those receiving oral cholera vaccines. we measured the avidity of antibodies to cholera toxin b subunit (ctb) and vibrio cholerae o1 lipopolysaccharide (lps) in bangladeshi adult cholera patients (n = 30), as well as vaccinees (n = 30) after administration of two doses of a killed oral cholera vaccine. we assessed antibody a ... | 2013 | 23114701 |
| antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. | dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. in this context, the validations of the traditional claim that the leaf extract of d. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. the bacterial biofilms were quantified by crystal violet staining. among the 17 bacterial pathogens screened ... | 2012 | 23115018 |
| 1-alkyl-(n,n-dimethylamino)pyridinium bromides: inhibitory effect on virulence factors of candida albicans and on the growth of bacterial pathogens. | a homologous series of 1-alkyl-(n,n-dimethylamino)pyridinium bromides, termed compounds 1-11, was synthesized and studied for antibacterial and antifungal activity. of these, compound 8, containing a ten-carbon alkyl chain, showed maximum inhibition against all the tested bacterial strains. the highest antibacterial activity using a disc diffusion method was recorded against mycobacterium smegmatis [zone of inhibition (zoi): 45.75±0.25 mm], followed by escherichia coli, proteus mirabilis, vibrio ... | 2013 | 23118472 |
| development of a groel gene-based species-specific multiplex polymerase chain reaction assay for simultaneous detection of vibrio cholerae, vibrio parahaemolyticus and vibrio vulnificus. | to develop an effective multiplex polymerase chain reaction (pcr) for the simultaneous detection of three important vibrio species, vibrio cholerae (vc), v. parahaemolyticus (vp) and v. vulnificus (vv) using the groel gene, a potential phylogenetic marker. | 2013 | 23121500 |
| bacterial quorum sensing: its role in virulence and possibilities for its control. | quorum sensing is a process of cell-cell communication that allows bacteria to share information about cell density and adjust gene expression accordingly. this process enables bacteria to express energetically expensive processes as a collective only when the impact of those processes on the environment or on a host will be maximized. among the many traits controlled by quorum sensing is the expression of virulence factors by pathogenic bacteria. here we review the quorum-sensing circuits of st ... | 2012 | 23125205 |
| a predictive model of vibrio cholerae for combined temperature and organic nutrient in aquatic environments. | to develop a predictive model for vibrio cholerae in sea water. | 2013 | 23126672 |