Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| infectious bronchitis virus population structure defines immune response and protection. | a commercial arkansas (ark) delmarva poultry industry (dpi)-type vaccine and a more homogeneous population of that vaccine obtained previously through adaptation to chicken embryo kidney (cek) cells (cek-arkdpi) were used as a model to further understand the impact of population genetic structure on generation of immune responses and protection. in a first experiment, vaccinated chickens were challenged with an ibv ark99-type virulent strain (al/4614/98). despite extensive sequence similarity be ... | 2020 | 32267126 |
| nationwide survey of infectious bronchitis virus on layer farms in japan during 2015. | after accumulating data through a nationwide survey, we characterized the recent prevalences and geographic distributions of various genotypes of infectious bronchitis virus (ibv) on layer farms in japan. reverse transcription pcr analysis of fecal samples revealed the presence of the ibv nucleoprotein (n) gene on approximately 30% of the farms surveyed. n-gene detection rates were higher in the chugoku and kyushu regions than in the remaining surveyed regions. phylogenetic analysis of s1 gene s ... | 2020 | 32267128 |
| infectious bronchitis virus immune responses in the harderian gland upon initial vaccination. | in recent years, arkansas delmarva poultry industry (arkdpi)-derived infectious bronchitis (ib) virus (ibv) vaccines have been used to characterize the immune responses of chickens subsequent to vaccination on day of hatch or beyond. perhaps because arkdpi vaccines display increased heterogeneity, the results on cell immune responses have shown ambiguity. in the current study, we investigated the effects of vaccination with a highly stable and homogeneous massachusetts (mass)-type vaccine on day ... | 2020 | 32267130 |
| new pa/1220/98-like variant of infectious bronchitis virus in poland. | the aim of the present study was to report the first detection of a new infectious bronchitis virus (ibv) variant in polish commercial flocks which is completely different to any previously known in this region. in 2018, samples from ross 308 breeding hens aged 35 weeks were delivered for ibv diagnosis. ibv presence was detected, but all attempts to amplify the s gene fragment were negative. the field material was analysed using the illumina miseq platform and a 1073-nt fragment of the s1 coding ... | 2020 | 32271091 |
| evaluation of protection conferred by a vaccination program based on the h120 and cr88 commercial vaccines against a field variant of avian infectious bronchitis virus. | due to serotype variations among different avian infectious bronchitis viruses isolated in tunisia since 2000, protection of chicks, especially broiler flocks, with mass h120 vaccine often fails. therefore, association of cr88 (793b type) with h120 vaccines was used for better response. challenge experiments were then conducted to evaluate tracheal and renal cross-protection in chickens immunized via nasal and eye drops. conferred protection was measured by clinical signs and macroscopic lesions ... | 2014 | 32288459 |
| efficacy of intranasal and spray delivery of adjuvanted live vaccine against infectious bronchitis virus in experimentally infected poultry. | live vaccines are widely used in the avian industry. such vaccines can be either injected or delivered on animal mucosa and are usually not adjuvanted. in this study we show that live vaccines efficacy can be improved by formulation with adjuvants in a model of mucosal delivery of live infectious bronchitis vaccine in chicken. three adjuvant technologies have been tested using intranasal and spray delivery methods to poultry. those technologies are water in oil in water emulsion, nanoparticles a ... | 2012 | 32288918 |
| current situation, genetic relationship and control measures of infectious bronchitis virus variants circulating in african regions. | infectious bronchitis virus (ibv) is a major viral pathogen of commercial poultry, affecting chickens of all ages and causing major economic losses in poultry industry worldwide. frequent points of mutations and recombination events in the s1 gene region, result in the emergence of new ibvs variants circulating in the form of several serotypes/genotypes that can be partially or poorly neutralized by current vaccines. ibv is well studied worldwide, nevertheless in african countries epidemiologica ... | 2016 | 32288944 |
| a duplex rt-pcr assay for detection of h9 subtype avian influenza viruses and infectious bronchitis viruses. | h9 subtype avian influenza virus (aiv) and infectious bronchitis virus (ibv) are major pathogens circulating in poultry and have resulted in great economic losses due to respiratory disease and reduced egg production. as similar symptoms are elicited by the two pathogens, it is difficult for their differential diagnosis. so far, no reverse transcription-polymerase chain reaction (rt-pcr) assay has been found to differentiate between h9 aiv and ibv in one reaction. therefore, developing a sensiti ... | 2016 | 32288952 |
| molecular characterization of infectious bronchitis viruses isolated from broiler and layer chicken farms in egypt during 2012. | one of the major problems of avian infectious bronchitis virus (ibv) is the frequent emergence of new variants. in the present study 205 tracheal swabs and organs were collected from broilers and layers chicken farms during january to august 2012 from 19 governorates all over egypt. the chickens demonstrated respiratory signs and mortality. out of the examined samples, 130 of which (about 64%) of suspected farms were positive for ibv with real time rt-pcr. 13 ibv-positive samples were selected f ... | 2013 | 32289036 |
| immunogenicity and efficacy of live infectious bronchitis 793/b.08ir vaccine in spf chickens. | infectious bronchitis virus (ibv) has a variety of serotypes with relatively limited cross-protection leading the disease to be a major problem in the poultry industry. the ibv 793/b strain has identified to circulate in iran; therefore, the development of a specific vaccine to protect against the virulent virus has received attention. in this regard, the live ib 793/b vaccine (793/b.08ir) was developed in the razi vaccine and serum research institute. in this study, the immunogenicity of 793/b. ... | 2020 | 32291999 |
| cocirculation of four infectious bronchitis virus lineages in broiler chickens in the eastern region of saudi arabia from 2012 to 2014. | avian infectious bronchitis virus (ibv) is an evolving and dynamic virus that causes major economic losses for the poultry industry worldwide. continuous evolution and emergence of new variants of this virus are the major challenges for controlling the disease with routine vaccination. successful vaccination usually requires the use of a homologous vaccine, which in turn necessitates continuous investigation of the circulating strains. herein, we performed a reverse transcriptase-polymerase chai ... | 2020 | 32292579 |
| decontamination of face masks with steam for mask reuse in fighting the pandemic covid-19: experimental supports. | the coronavirus disease 2019 pandemic caused by the novel coronavirus sars-cov-2 (severe acute respiratory syndrome coronavirus 2) has claimed many lives worldwide. wearing medical masks (mms) or n95 masks ([n95ms] namely n95 respirators) can slow the virus spread and reduce the infection risk. reuse of these masks can minimize waste, protect the environment, and help solve the current imminent shortage of masks. disinfection of used masks is needed for their reuse with safety, but improper deco ... | 2020 | 32320083 |
| in ovo delivered toll-like receptor 7 ligand, resiquimod enhances host responses against infectious bronchitis corona virus (ibv) infection. | toll-like receptor (tlr) 7 ligand, resiquimod, has been studied as an adjuvant and antiviral agent against several pathogens in chicken. yet, the effectiveness of resiquimod against infectious bronchitis virus (ibv) infection has not been evaluated. in this study, we investigated the effectiveness of resiquimod delivered pre-hatch (in ovo) against ibv infection post-hatch identifying key mechanisms involved in resiquimod driven immune activation. first, we found an upregulation of interleukin (i ... | 2020 | 32326430 |
| phylodynamic analysis and evaluation of the balance between anthropic and environmental factors affecting ibv spreading among italian poultry farms. | infectious bronchitis virus (ibv) control is mainly based on wide vaccine administration. although effective, its efficacy is not absolute, the viral circulation is not prevented and some side effects cannot be denied. despite this, the determinants of ibv epidemiology and the factors affecting its circulation are still largely unknown and poorly investigated. in the present study, 361 ibv qx (the most relevant field genotype in italy) sequences were obtained between 2012 and 2016 from the two m ... | 2020 | 32350378 |
| development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus. | infectious laryngotracheitis disease is an acute, highly contagious viral disease seriously affecting poultry industry worldwide. in this study, a rapid and simple immune colloidal gold test strip for detecting infectious laryngotracheitis virus (iltv) was developed based on membrane chromatography with monoclonal antibodies (mabs) against gj protein of iltv and systematically evaluated for the detection of iltv from clinical samples. mab 2d4 1d7 was conjugated with colloidal gold as the detecto ... | 2020 | 32359575 |
| natural product-derived phytochemicals as potential agents against coronaviruses: a review. | coronaviruses are responsible for a growing economic, social and mortality burden, as the causative agent of diseases such as severe acute respiratory syndrome (sars), middle east respiratory syndrome (mers), avian infectious bronchitis virus (ibv) and covid-19. however, there is a lack of effective antiviral agents for many coronavirus strains. naturally existing compounds provide a wealth of chemical diversity, including antiviral activity, and thus may have utility as therapeutic agents again ... | 2020 | 32360300 |
| high-resolution melting curve analysis for infectious bronchitis virus strain differentiation. | belonging to the coronaviridae family, avian infectious bronchitis virus (ibv) causes respiratory, reproductive, and renal diseases in poultry. preventative measures lie mainly in vaccination, while the gold standard for ibv classification and differentiation is based on the sequence analysis of the spike 1 (s1) gene. in this study, we tested a new assay for ibv strain classification that is less expensive and requires reduced time and effort to perform. we carried out a quantitative real-time p ... | 2020 | 32367941 |
| emergence of variant avian infectious bronchitis virus in india. | infectious bronchitis virus (ibv) is the etiological agent of an acute and highly contagious disease. infectious bronchitis (ib) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. the continuous evolution of the spike protein (s1) of ibv is responsible for the prevalence of many serotypes/genotypes around the world. multiple lineages of ibv strains have been detected in chicken flocks in india since 2003. | 2020 | 32368223 |
| spotlight on avian coronaviruses. | coronaviruses (covs) mainly cause enteric and/or respiratory signs. mammalian covs including covid-19 (now officially named sars-cov-2) belong to either the alphacoronavirus or betacoronavirus genera. in birds, the majority of the known covs belong to the gammacoronavirus genus, whilst a small number are classified as deltacoronaviruses. gammacoronaviruses continue to be reported in an increasing number of avian species, generally by detection of viral rna. apart from infectious bronchitis virus ... | 2020 | 32374218 |
| a highly pathogenic gi-19 lineage infectious bronchitis virus originated from multiple recombination events with broad tissue tropism. | in the present study, an ibv strain i0305/19 was isolated from a diseased commercial broiler flock in 2019 in china with high morbidity and mortality. the isolate i0305/19 was clustered together with viruses in sublineage d of gi-19 lineage on the basis of the complete s1 sequence analysis. isolate i0305/19 and other gi-19 viruses isolated in china have the amino acid sequence mia at positions 110-112 in the s protein. further analysis based on the complete genomic sequence showed that the isola ... | 2020 | 32380209 |
| immunosensor based on antibody-functionalized mos2 for rapid detection of avian coronavirus on cotton thread. | infectious bronchitis virus (ibv), an avian coronavirus, significantly affects the performance of both the egg-laying and meat-type birds causing the foremost of economic loss in poultry industry. this paper aims to develop a rapid, low-cost, and sensitive biosensor for ibv detection by using molybdenum disulfide (mos2). mos2 is a 2-d nanosheet which has strong high fluorescence-quenching ability when applied to a dye-labeled antibody (ab). in this paper, we developed an ab-functionalized mos2-b ... | 2018 | 32390783 |
| pathogenicity of a qx-like avian infectious bronchitis virus isolated in china. | avian infectious bronchitis is a serious and highly contagious disease caused by infectious bronchitis virus (ibv). we isolated a highly virulent ibv strain (ck/ch/js/tahy) from kidneys of diseased chickens. phylogenetic analysis based on the s1 gene revealed that ck/ch/js/tahy clustered with the qx-like type. the s1 gene has 1,620 nucleotides and encoded a polypeptide of 540 amino acids with typical coronavirus cleavage recognition sites of hrrr. about 1-day-old specific pathogen-free white leg ... | 2020 | 32416792 |
| reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus. | the study was conducted to develop a specific, simple, and sensitive method for diagnosis of avian infectious bronchitis virus (ibv). in this experiment, the selected downstream primer was labeled with biotin and the 5' end of raa probe was labeled with fam by reverse transcription recombinase-aided amplification (rt-raa) combined with lateral flow dipstick (lfd). a rt-raa-lfd assay that could be used for detection of ibv was established after optimization of rt-raa reaction time, reaction tempe ... | 2020 | 32416856 |
| immunomodulatory effect of ginseng stem-leaf saponins and selenium on harderian gland in immunization of chickens to newcastle disease vaccine. | our previous study demonstrated that ginseng stem-leaf saponins (gsls) in combination with selenium (gsls-se) have adjuvant effect on the live vaccine of newcastle disease virus (ndv) and infectious bronchitis virus (ibv) in intraocular-and-intranasal immunization in chickens. the present study was to investigate the potential molecular mechanisms involved in the immunomodulation of gsls-se on the harderian gland (hg). it was found that the window allowing animals susceptible to infections due t ... | 2020 | 32422443 |
| infectious bronchitis virus regulates cellular stress granule signaling. | viruses must hijack cellular translation machinery to express viral genes. in many cases, this is impeded by cellular stress responses. these stress responses result in the global inhibition of translation and the storage of stalled mrnas, into rna-protein aggregates called stress granules. this results in the translational silencing of the majority of mrnas excluding those beneficial for the cell to resolve the specific stress. for example, the expression of antiviral factors is maintained duri ... | 2020 | 32422883 |
| pustulan activates chicken bone marrow-derived dendritic cells in vitro and promotes ex vivo cd4+ t cell recall response to infectious bronchitis virus. | infectious bronchitis virus (ibv) is a highly contagious avian coronavirus. ibv causes substantial worldwide economic losses in the poultry industry. vaccination with live-attenuated viral vaccines, therefore, are of critical importance. live-attenuated viral vaccines, however, exhibit the potential for reversion to virulence and recombination with virulent field strains. therefore, alternatives such as subunit vaccines are needed together with the identification of suitable adjuvants, as subuni ... | 2020 | 32429204 |
| characterization of novel, pathogenic field strains of infectious bronchitis virus (ibv) in poultry in trinidad and tobago. | avian coronaviruses, including infectious bronchitis virus (ibv) and turkey coronavirus (tcov), are economically important viruses affecting poultry worldwide. ibv is responsible for causing severe losses to the commercial poultry sector globally. the objectives of this study were to identify the viruses that were causing outbreaks of severe respiratory disease in chickens in trinidad and tobago (t&t) and to characterize the strains. swab samples were collected from birds showing severe respirat ... | 2020 | 32438523 |
| a novel protein chip for simultaneous detection of antibodies against four epidemic swine viruses in china. | at present, pig industry in china is faced with the complex situation of mixed infection caused by multiple pathogens. it is urgent to develop some new high-throughput molecular diagnosis assays to simultaneously detect pathogens or antibodies. biochip array technology has made it possible to screen thousands of samples simultaneously; it has been twice named as one of the top 10 scientific and technological breakthroughs. studies have reported encouraging results using protein biochips for dete ... | 2020 | 32456688 |
| molecular characterization of infectious bronchitis virus based on rna-dependent rna polymerase gene. | extensive rate of variations in the s1 gene (spike glycoprotein subunit gene) of infectious bronchitis virus (ibv) causes challenges for clinicians in counting variants for differentiation of infected from vaccinated birds and addressing the variants of unknown significance. this study investigated the possibility of using an rna-dependent rna polymerase gene (rdrp) as a target for molecular characterization of ibv strains in iran. trachea samples were collected from commercial broiler flocks (n ... | 2020 | 32458483 |
| adjuvant effects of platycodin d on immune responses to infectious bronchitis vaccine in chickens. | adjuvants are common vaccine components. novel adjuvants may improve the protective immunity conferred by vaccines against poultry diseases. here, a less-hemolytic saponin, platycodin d (pd), isolated from the root of platycodon grandiflorum was investigated as a potential alternative adjuvant. pd was tested as an adjuvant in the infectious bronchitis (ib) vaccine, because the existing ib vaccine has often failed to induce effective immune responses. the adjuvant activity of pd in conjunction wi ... | 2020 | 32461731 |
| molecular and pathogenicity of infectious bronchitis virus (gammacoronavirus) in japanese quail (coturnix japonica). | infectious bronchitis virus (ibv) infection is highly infectious respiratory disease in poultry industry with significant economic importance. the prevalence of ibv in quail industry in malaysia was not well documented; therefore, its actual role in the epidemiology of the disease is relatively unknown. this study was to determine the susceptibility of japanese quail, as one of the species in commercial poultry industry, toward ibv. in addition, it will also give a potential impact on the overal ... | 2020 | 32475428 |
| epidemiological investigation of avian infectious bronchitis and locally determined genotype diversity in central china: a 2016-2018 study. | infectious bronchitis (ib), caused by avian ib virus (ibv), is an acute and highly contagious disease of chickens. from 2016 to 2018, 56 ibv strains were isolated and identified from clinical samples obtained from various chicken farms located in central china. the s1 sequencing of these strains revealed nucleotide and amino acid identities of 70.2 to 100% and 62.6 to 100%, respectively, compared with those of reference strains. phylogenetic analysis indicated that the genotypes of the isolates ... | 2020 | 32475435 |
| evolutionary analysis of infectious bronchitis virus reveals marked genetic diversity and recombination events. | in the last 5 years, frequent outbreaks of infectious bronchitis virus (ibv) are observed in both broiler and layer chicken flocks in the kingdom of saudi arabia (ksa) in spite of extensive usage of vaccines. the ibv is a widespread avian coronavirus affecting both vaccinated and unvaccinated chicken flocks and is attributed to significant economic losses, around the globe. in the present study, 58 (n = 58) samples were collected from four different commercial poultry flocks from 8 ksa districts ... | 2020 | 32486006 |
| induction of robust immune responses by cpg-odn-loaded hollow polymeric nanoparticles for antiviral and vaccine applications in chickens. | poultry vaccine has limited choices of adjuvants and is facing severe threat of infectious diseases due to ineffective of widely used commercial vaccines. thus, development of novel adjuvant that offers safe and effective immunity is of urgent need. | 2020 | 32494131 |
| evaluation of inactivated vaccine of the variant 2 (is-1494 /gi-23) genotype of avian infectious bronchitis. | the infectious bronchitis virus (ibv) is the cause of avian infectious bronchitis (ib). ib is one of the most highly contagious diseases, which results in many economic losses in the poultry industry worldwide. the nature of this virus is such that it generates new genotypes continuously. proper vaccination is the most suitable way of combatting ib. one of the novel genotypes of ibv, which has been circulating in the middle eastern countries, is the variant 2 (is-1494/gi-23) genotype. this study ... | 2020 | 32505764 |
| a unifying structural and functional model of the coronavirus replication organelle: tracking down rna synthesis. | zoonotic coronavirus (cov) infections, such as those responsible for the current severe acute respiratory syndrome-cov 2 (sars-cov-2) pandemic, cause grave international public health concern. in infected cells, the cov rna-synthesizing machinery associates with modified endoplasmic reticulum membranes that are transformed into the viral replication organelle (ro). although double-membrane vesicles (dmvs) appear to be a pan-cov ro element, studies to date describe an assortment of additional cov ... | 2020 | 32511245 |
| changes in the percentages of b- and t-lymphocytes and antibody titres in laying hens infested with dermanyssus gallinae-a preliminary study. | (1) background: dermanyssus gallinae, a hematophagous ectoparasite, adversely affects the health status of laying hens, leading to reduced egg production and significant economic losses in commercial farms. the aim of this study was to determine the effect of d. gallinae on the development of post-vaccination immune responses in layer hens. (2) methods: a total of 80 blood samples were collected at four time-points (b1-b4) from 10 hy-line brown hens, randomly selected from a commercial layer far ... | 2020 | 32517104 |
| the construction and immunoadjuvant activities of the oral interleukin-17b expressed by lactobacillus plantarum nc8 strain in the infectious bronchitis virus vaccination of chickens. | interleukin-17b (il-17b) is a protective cytokine of the il-17 family and plays an essential role in the regulation of mucosal inflammation. however, little is known about the role of il-17b in the control of viral infections. in this study, a recombinant lactobacillus plantarum, designated as nc8-chil17b, was constructed to express the chicken il-17b (chil-17b) gene. the recombinant chil17b (rchil17b) protein was about 14 kda and was anchored to the surface of nc8 cells. in vitro, it was found ... | 2020 | 32517220 |
| efficacy of infectious bronchitis gi-13 (793b) vaccine candidate tested according to the current european union requirements and for cross-protection against heterologous qx-like challenge. | infectious bronchitis (ib) is a highly contagious viral disease of chickens, known to cause severe economic losses. vaccination against ib virus (ibv) is an important control measure against the disease. the objective of the present study was to test avishield ib gi-13, the vaccine candidate against ibv, strain v-173/11 (gi-13 genotype), according to european pharmacopoeia (ph. eur.) efficacy requirements. laboratory study on specific-pathogen-free (spf) chickens showed 100% protection against c ... | 2020 | 32522104 |
| infectious bronchitis virus inhibits activation of the tlr7 pathway, but not the tlr3 pathway. | various strains of infectious bronchitis virus (ibv) cause different forms of infectious bronchitis with different clinical signs. here, primary chicken embryo kidney (cek) cells and specific-pathogen-free (spf) chickens were infected with three pathogenic ibv strains, and it was observed that the tlr7-myd88 pathway was inhibited but the tlr3-tirf pathway was activated. after treatment with poly(i:c)-lmw, poly (i:c)-lmw/lyovec, and imiquimod, the replication of ibv was significantly suppressed a ... | 2020 | 32524263 |
| health status of free-ranging ring-necked pheasant chicks (phasianus colchicus) in north-western germany. | being a typical ground-breeding bird of the agricultural landscape in germany, the pheasant has experienced a strong and persistent population decline with a hitherto unexplained cause. contributing factors to the ongoing negative trend, such as the effects of pesticides, diseases, predation, increase in traffic and reduced fallow periods, are currently being controversially discussed. in the present study, 62 free-ranging pheasant chicks were caught within a two-year period in three federal sta ... | 2020 | 32544211 |
| diagnostic accuracy of ultrasonography to detect false layers in a commercial laying flock infected by an infectious bronchitis virus delmarva genotype causing cystic oviducts. | infection of the oviduct by an infectious bronchitis virus (ibv) in laying hens has been associated with the false layer syndrome. because the diagnostic procedure for the detection of cystic oviducts by postmortem examinations in ibv-positive replacement pullet flocks could involve the unnecessary sacrifice of numerous healthy pullets without reproductive tract anomalies, the development of a noninvasive and nonlethal diagnostic procedure would be desirable. the first objective of the study was ... | 2020 | 32550615 |
| infectious coryza: persistence, genotyping, and vaccine testing. | the reemergence of infectious coryza (ic) caused by avibacterium paragallinarum (ap) as an acute and occasionally chronic respiratory disease in domestic poultry has caused severe losses in several u.s. states. the disease is also associated with decreased egg production in layers and increased condemnations from air sac infections in broilers. a series of applied experiments were performed to elucidate the persistence of ap in infected broiler flocks, to genotype ap strains isolated from field ... | 2020 | 32550616 |
| genetic, antigenic, and pathogenic characteristics of infectious bronchitis virus gi-7/tw-ii in china. | nine infectious bronchitis virus (ibv) strains belonging to the gi-7 lineage were isolated between 2009 and 2017 in china. phylogenetic analysis and comparisons of full-length sequences of the s1 gene suggested that the gi-7 lineage should be further classified as taiwan (tw)-i and tw-ii sublineages, which correspond to the previous tw-i and tw-ii genotypes. the nine ibv strains were clustered in the tw-ii sublineage. further investigation revealed that viruses in the tw-i and tw-ii were not onl ... | 2020 | 32550619 |
| limited cross-protection against infectious bronchitis provided by recombinant infectious bronchitis viruses expressing heterologous spike glycoproteins. | gammacoronavirus infectious bronchitis virus (ibv) causes an economically important respiratory disease of poultry. protective immunity is associated with the major structural protein, spike (s) glycoprotein, which induces neutralising antibodies and defines the serotype. cross-protective immunity between serotypes is limited and can be difficult to predict. in this study, the ability of two recombinant ibv vaccine candidates, beaur-m41(s) and beaur-4/91(s), to induce cross-protection against a ... | 2020 | 32580371 |
| infectious bronchitis virus evolution, diagnosis and control. | rna viruses are characterized by high mutation and recombination rates, which allow a rapid adaptation to new environments. most of the emerging diseases and host jumps are therefore sustained by these viruses. rapid evolution may also hinder the understanding of molecular epidemiology, affect the sensitivity of diagnostic assays, limit the vaccine efficacy and favor episodes of immune escape, thus significantly complicating the control of even well-known pathogens. the history of infectious bro ... | 2020 | 32580381 |
| nitric oxide production and fc receptor-mediated phagocytosis as functional readouts of macrophage activity upon stimulation with inactivated poultry vaccines in vitro. | vaccine batches must pass routine quality control to confirm that their ability to induce protection against disease is consistent with batches of proven efficacy from development studies. for poultry vaccines, these tests are often performed in laboratory chickens by vaccination-challenge trials or serological assays. the aim of this study was to investigate innate immune responses against inactivated poultry vaccines and identify candidate immune parameters for in vitro quality tests as altern ... | 2020 | 32580391 |
| comparative protective efficacies of novel avian paramyxovirus-vectored vaccines against virulent infectious bronchitis virus in chickens. | viral vectored vaccines are desirable alternatives for conventional infectious bronchitis virus (ibv) vaccines. we have recently shown that a recombinant newcastle disease virus (rndv) strain lasota expressing the spike (s) protein of ibv strain mass-41 (rlasota/ibv-s) was a promising vaccine candidate for ibv. here we evaluated a novel chimeric rndv/avian paramyxovirus serotype 2 (rndv/apmv-2) as a vaccine vector against ibv. the rndv/apmv-2 vector was chosen because it is much safer than the r ... | 2020 | 32605292 |
| molecular detection of avian infectious bronchitis viruses in live bird markets, gilan province. | coronaviruses (avcov) which include infectious bronchitis virus (ibv) and other bird coronaviruses belong to the genus gammacoronavirus, subfamily coronavirinae. one of the most prominent representatives of gammacoronavirus genus is infectious bronchitis virus (ibv) which is a highly contagious viral pathogen of chickens causing considerable economic losses to the poultry industry. ibvs mostly affect the respiratory, urinary, and reproductive tracts leading to a substantial drop in production. b ... | 2020 | 32621443 |
| molecular monitoring of d1466 genotype of avian infectious bronchitis virus in iran: a retrospective study in 2013-2017. | infectious bronchitis is one of the most common diseases in the poultry industry in many countries, especially in the regions with a dense poultry farming industry. gammacoronavirus is the etiologic agent of this disease, with the chickens and poultries as the natural reservoirs of the virus. various strains of infectious bronchitis virus have been reported in poultry around the world. in terms of pathogenicity, this virus can induce a spectrum of diseases ranging from the moderate respiratory t ... | 2020 | 32621444 |
| emerging infectious bronchitis virus (ibv) in egypt: evidence for an evolutionary advantage of a new s1 variant with a unique gene 3ab constellation. | infectious bronchitis virus (ibv), a gamma-coronavirus, causes infectious bronchitis (ib), a major respiratory disease of chicken. its high mutation rate in conjunction with recombination of the rna genome constantly creates ibv variants that are difficult to control by currently available vaccines. in this study, we addressed the question whether small-scale holdings might harbor ibv variants that serve as a reservoir for newly emerging variants. egyptian ibv isolate egy/nr725/2016 (nr725/16) f ... | 2020 | 32622080 |
| pathogenic characteristics of a qx-like infectious bronchitis virus strain sd in chickens exposed at different ages and protective efficacy of combining live homologous and heterologous vaccination. | continued reports of infections with infectious bronchitis virus (ibv) variants have occurred since its first isolation in the 1930s. currently, qx-like ibvs are the predominant circulating genotype around the world. here, the pathogenicity of qx-like ibv strain sd was characterized in chickens at different ages of exposure to the virus, and the protection efficacy of available vaccine combinations against ibv was evaluated. the results revealed that qx-like ibv strain sd was severely pathogenic ... | 2020 | 32641162 |
| a novel mucosal adjuvant system for immunization against avian coronavirus causing infectious bronchitis. | infectious bronchitis (ib) caused by infectious bronchitis virus (ibv) is currently a major threat to chicken health, with multiple outbreaks being reported in the united states over the past decade. modified live virus (mlv) vaccines used in the field can persist and provide the genetic material needed for recombination and emergence of novel ibv serotypes. inactivated and subunit vaccines overcome some of the limitations of mlv with no risk of virulence reversion and emergence of new virulent ... | 2020 | 32669327 |
| wild birds as reservoirs for diverse and abundant gamma- and deltacoronaviruses. | wild birds interconnect all parts of the globe through annual cycles of migration with little respect for country or continental borders. although wild birds are reservoir hosts for a high diversity of gamma- and deltacoronaviruses, we have little understanding of the ecology or evolution of any of these viruses. in this review, we use genome sequence and ecological data to disentangle the evolution of coronaviruses in wild birds. specifically, we explore host range at the levels of viral genus ... | 2020 | 32672814 |
| temperature sensitivity: a potential method for the generation of vaccines against the avian coronavirus infectious bronchitis virus. | the gammacoronavirus infectious bronchitis virus (ibv) is a highly contagious economically important respiratory pathogen of domestic fowl. reverse genetics allows for the molecular study of pathogenic determinants to enable rational vaccine design. the recombinant ibv (ribv) beau-r, a molecular clone of the apathogenic beaudette strain, has previously been investigated as a vaccine platform. to determine tissues in which beau-r could effectively deliver antigenic genes, an in vivo study in chic ... | 2020 | 32674326 |
| a closed-tube, single-step, real time, reverse transcription-loop-mediated isothermal amplification assay for infectious bronchitis virus detection in chickens. | infectious bronchitis (ib) is a viral infection of the chicken respiratory tract that causes substantial economic burden on the industry. simple, specific and rapid diagnosis of this disease is critical for the initiation of appropriate control measures. conventional molecular diagnostic methods require a relatively sophisticated equipment and skilled staff. here we describe a rapid, simple, semi-quantative, closed-tube, single-step, real-time- reverse transcription-loop-mediated isothermal ampl ... | 2020 | 32687868 |
| sequence analysis and pathogenicity of avian orthoavulavirus 1 strains isolated from poultry flocks during 2015-2019. | newcastle disease (nd) causes severe economic losses in poultry industry worldwide. egyptian poultry industry suffered from severe economic losses since the isolation of velogenic newcastle disease virus (vndv) genotype viid in 2011 and up till now despite the use of different vaccination programs. so, this study aimed to isolate and characterize the vndv from a total of 120 poultry flocks from ten provinces in the egyptian delta region with a history of respiratory manifestation, high mortaliti ... | 2020 | 32698866 |
| effect of restricted feed intake in broiler breeder hens on their stress levels and the growth and immunology of their offspring. | the prenatal environment has been shown to have significant effects on the lifelong health of offspring in humans and other species. such effects have not been studied extensively in avian species but could prove important, especially in the case of severe feed restriction imposed on broiler breeder hens to prevent obesity and reduce rate of lay. feed restriction can potentially affect not only nutrient supply to the embryo but stress hormone levels within the hen. this study investigated the im ... | 2018 | 32704710 |
| multiple novel non-canonically transcribed sub-genomic mrnas produced by avian coronavirus infectious bronchitis virus. | coronavirus sub-genomic mrna (sgmrna) synthesis occurs via a process of discontinuous transcription involving complementary transcription regulatory sequences (trss), one (trs-l) encompassing the leader sequence of the 5' untranslated region (utr), and the other upstream of each structural and accessory gene (trs-b). several coronaviruses have an orf located between the n gene and the 3'-utr, an area previously thought to be non-coding in the gammacoronavirus infectious bronchitis virus (ibv) du ... | 2020 | 32720890 |
| evaluation of the reproductive system development and egg-laying performance of hens infected with tw i-type infectious bronchitis virus. | the prevalence of tw i-type infectious bronchitis virus (ibv) has been increasing rapidly, and it has become the second most common genotype of ibv in china threatening the poultry industry. in this study, 1-day-old specific-pathogen-free (spf) chickens infected with tw i-type ibv were continuously observed for 200 days. tw i-type ibv affected the respiratory, urinary, and female reproductive systems, resulting in a mortality rate of 10% as well as a decrease in egg quantity and an increase in i ... | 2020 | 32736651 |
| risks and disease aetiologies of compromised performance in commercial broilers kept at lower stocking density and limited antimicrobial use. | the health status of broilers raised at lower stocking density and limited antimicrobial use (but routine anticoccidials) was assessed in order to identify prevalent causes of disease, mortality and reduced performance. "dead-on-farm"(dof) broilers from 145 commercial flocks were investigated at two different time points (tp1:7-14 and tp2:28-35 days of age); per sampling, 6-10 dof broilers were selected for post-mortem investigation and gross pathomorphological changes were assessed from 2717 bi ... | 2020 | 32746625 |
| heat shock protein member 8 is an attachment factor for infectious bronchitis virus. | although infectious bronchitis virus (ibv) is the first coronavirus identified, little is known about which membrane protein of host cells could interact with ibv spike protein and facilitate the infection by the virus. in this study, by using a monoclonal antibody to the s1 protein of ibv m41 strain, we found that heat shock protein member 8 (hspa8) could interact with spike protein of ibv. hspa8 was found to be present on the cell membrane and chicken tissues, with highest expression level in ... | 2020 | 32765462 |
| the characterization of chifitms in avian coronavirus infection in vivo, ex vivo and in vitro. | the coronaviruses are a large family of enveloped rna viruses that commonly cause gastrointestinal or respiratory illnesses in the infected host. avian coronavirus infectious bronchitis virus (ibv) is a highly contagious respiratory pathogen of chickens that can affect the kidneys and reproductive systems resulting in bird mortality and decreased reproductivity. the interferon-inducible transmembrane (ifitm) proteins are activated in response to viral infections and represent a class of cellular ... | 2020 | 32785186 |
| construction of an infectious bronchitis virus vaccine strain carrying chimeric s1 gene of a virulent isolate and its pathogenicity analysis. | infectious bronchitis virus (ibv) is a member of genus gamma-coronavirus in the family coronaviridae, causing serious economic losses to the poultry industry. reverse genetics is a common technique to study the biological characteristics of viruses. so far, there is no bac reverse genetic system available for rescue of ibv infectious clone. in the present study, a new strategy for the construction of ibv infectious cdna clone was established. the full-length genomic cdna of ibv vaccine strain h1 ... | 2020 | 32813067 |
| whole-genome sequencing protocols for ibv and other coronaviruses using high-throughput sequencing. | this chapter reports the high-throughput sequencing protocol for sequencing coronaviruses and other positive strand viruses to produce a dataset of significant depth of coverage. the protocol describes sequencing of infectious bronchitis virus propagated in embryonated eggs and harvested in the allantoic fluid. the protocol is composed of three main steps-enrichment of the allantoic fluid using ultracentrifugation, extraction of total rna from allantoic fluid, and library preparation from total ... | 2020 | 32833204 |
| isolation and propagation of coronaviruses in embryonated eggs. | the embryonated egg is a complex structure comprised of an embryo and its supporting membranes (chorioallantoic, amniotic, and yolk). the developing embryo and its membranes provide a diversity of cell types that allow for the successful replication of a wide variety of different viruses. within the family coronaviridae the embryonated egg has been used as a host system primarily for two avian coronaviruses within the genus gammacoronavirus, infectious bronchitis virus (ibv) and turkey coronavir ... | 2020 | 32833208 |
| transient dominant selection for the modification and generation of recombinant infectious bronchitis coronaviruses. | we have developed a reverse genetics system for the avian coronavirus infectious bronchitis virus (ibv) in which a full-length cdna corresponding to the ibv genome is inserted into the vaccinia virus genome under the control of a t7 promoter sequence. vaccinia virus as a vector for the full-length ibv cdna has the advantage that modifications can be introduced into the ibv cdna using homologous recombination, a method frequently used to insert and delete sequences from the vaccinia virus genome. ... | 2020 | 32833211 |
| sequencing and phylogenetic analysis of infectious bronchitis virus variant strain from an outbreak in egg-layer flocks in baghdad, iraq. | infectious bronchitis (ib) has an influential economic impact on the poultry industry, causing huge losses each year due to the condemnation of infected chickens. despite the use of many kinds of vaccines in iraq, it is common to find ib problems in vaccinated chickens. information about the strains that affect iraqi chickens is very limited. therefore, we aimed to detect the currently circulating strains of ib virus that cause frequent outbreaks in egg layers despite the use of vaccination agai ... | 2020 | 32848311 |
| replicase 1a gene plays a critical role in pathogenesis of avian coronavirus infectious bronchitis virus. | avian coronavirus infectious bronchitis virus (ibv) is an important pathogen threatening poultry production worldwide. here, two recombinant ibvs (ryn-1a-ayn and ryn-1b-ayn) were generated in which orf1a or orf1b of the virulent yn genome were replaced by the corresponding regions from the attenuated strain ayn. the pathogenicity and virulence of ribvs were evaluated in ovo and in vivo. the results revealed that mutations in the orf1a gene during passage in embryonated eggs caused the decreased ... | 2020 | 32853833 |
| vaccine design of coronavirus spike (s) glycoprotein in chicken: immunoinformatics and computational approaches. | infectious bronchitis (ib) is a highly contagious respiratory disease in chickens and produces economic loss within the poultry industry. this disease is caused by a single stranded rna virus belonging to cronaviridae family. this study aimed to design a potential multi-epitopes vaccine against infectious bronchitis virus spike protein (s). protein characterization was also performed for ibv spike protein. | 2020 | 32869000 |
| molecular identification of infectious bronchitis virus isolated from respiratory diseases in some iranian broiler flocks. | infectious bronchitis (ib) is an acute, highly contagious disease associated with respiratory signs in young chickens and reduced egg production and quality in layers. the purpose of this study was to isolate and identify the infectious bronchitis virus in broiler flocks with respiratory diseases in four provinces of iran. the specimens from forty ib suspected flocks from different regions of isfahan, east azerbaijan, golestan, and khuzestan provinces were collected, and the trachea, lung, and c ... | 2020 | 32892307 |
| molecular survey of respiratory and immunosuppressive pathogens associated with low pathogenic avian influenza h9n2 subtype and virulent newcastle disease viruses in commercial chicken flocks. | the study was carried out in 48 poultry flocks to elucidate the roles of various complicating pathogens involved along with newcastle disease (nd)/ low pathogenic avian influenza (lpai) outbreaks. necropsy was conducted and samples were collected for the isolation of newcastle disease virus (ndv), influenza a virus, infectious bronchitis virus (ibv), pathogenic bacteria; molecular detection of infectious laryngotracheitis virus (iltv), fowl adeno virus (fav), chicken anaemia virus (cav), mycopla ... | 2017 | 32908424 |
| characterization of s1 gene sequence variations of attenuated qx-like and variant infectious bronchitis virus strains and the pathogenicity of the viruses in specific-pathogen-free chickens. | besides the vaccine strains, the malaysian variant (mv) and qx-like are the predominant ibvs detected on commercial poultry farms. these two virus strains are distinct based on genomic and pathogenicity studies. in this study, we determined the sequence of the s1 gene and compared the pathogenicity of serial passage 70 (p70) of malaysian qx-like (qx/p70) and mv (mv/p70) strains with that of their respective wild-type viruses. the nucleotide and amino acid sequences of the complete s1 genes of qx ... | 2020 | 32964293 |
| coronavirus interactions with the cellular autophagy machinery. | the covid-19 pandemic, caused by the sars-cov-2 virus, is the most recent example of an emergent coronavirus that poses a significant threat to human health. virus-host interactions play a major role in the viral life cycle and disease pathogenesis, and cellular pathways such as macroautophagy/autophagy prove to be either detrimental or beneficial to viral replication and maturation. here, we describe the literature over the past twenty years describing autophagy-coronavirus interactions. there ... | 2020 | 32964796 |
| immunopathogenesis of infectious bronchitis virus q1 in specific pathogen free chicks. | the immunopathogenesis of avian coronavirus, infectious bronchitis virus (ibv) q1, was investigated in specific pathogen free chicks. following infection, chicks exhibited respiratory clinical signs and reduced body weight. oropharyngeal (op) and cloacal (cl) swabs were collected at intervals and found to be rt-pcr positive, with a greater number of partial-s1 amino acid changes noted in cl swabs compared to op swabs. in tissue samples, ibv viral load peaked 9 days post infection (dpi) in the tr ... | 2020 | 32980469 |
| development of hibit-tagged recombinant infectious bronchitis coronavirus for efficient in vitro and in vivo viral quantification. | coronaviruses (covs) are enveloped (+) ssrna viruses of veterinary and medical importance. because recombinant covs with reporter proteins fused with viral proteins are usually non-viable or unstable, a small and quantifiable epitope tag would be beneficial to cov research. in this study, we integrated the nanoluc binary technology to the reverse genetics of infectious bronchitis virus (ibv), a prototypic gammacoronavirus. the 11-amino-acid hibit tag was inserted to the spike (s) or membrane (m) ... | 2020 | 32983065 |
| infectious bronchitis coronavirus infection in chickens: multiple system disease with immune suppression. | in the early 1930s, infectious bronchitis (ib) was first characterized as a respiratory disease in young chickens; later, the disease was also described in older chickens. the etiology of ib was confirmed later as being due to a coronavirus: the infectious bronchitis virus (ibv). being a coronavirus, ibv is subject to constant genome change due to mutation and recombination, with the consequence of changing clinical and pathological manifestations. the potential use of live attenuated vaccines f ... | 2020 | 32987684 |
| efficacy of commercial polyvalent avian infectious bronchitis vaccines against chinese qx-like and tw-like strain via different vaccination strategies. | the infectious bronchitis virus (ibv) is an acute and highly contagious disease, which affects chickens of all ages. vaccination is the most important way to control this disease. nevertheless, novel variant strains are constantly reported because of the lack of proofreading capabilities of rna polymerase and high frequency of homologous rna recombination. cross-protection studies has demonstrated that the vaccines could provide great protective effects against viruses of same serotype or genoty ... | 2020 | 32988513 |
| research note: rapid detection of avian infectious laryngotracheitis virus with real-time fluorescence-based recombinase-aided amplification. | in this study, specific primers and fluorescent probes were designed to target the thymidine kinase (tk) gene sequence of avian infectious laryngotracheitis virus (iltv). through specificity and sensitivity tests, a real-time fluorescence-based recombinase-aided amplification (rf-raa) method for detecting iltv was established. the results showed that the method was specific and could be used to accurately detect iltv, and there was no cross-reaction with newcastle disease virus (ndv), avian infl ... | 2020 | 32988516 |
| a bio-safe multiple antigenic peptide (map) enzyme-linked immunoassay for the detection of antibodies to infectious bronchitis virus in chickens. | the objective of the study was to develop a bio-safe synthetic peptide elisa for the detection of antibodies against the infectious bronchitis virus (ibv) using a novel multiple antigenic peptide approach (map). after initial elisa optimization, diagnostic sensitivity (dsn) and specificity (dsp) for the linear peptides were determined using receiver operator curve (roc) analysis. the peptide ibvp1 showed 90.44% dsn and 88.64% dsp at roc cut off 22.8% while ibvp2 showed 88.24% dsn and 85.23% dsp ... | 2020 | 32999814 |
| treatment with exogenous trypsin expands in vitro cellular tropism of the avian coronavirus infectious bronchitis virus. | the gammacoronavirus infectious bronchitis virus (ibv) causes a highly contagious and economically important respiratory disease in poultry. in the laboratory, most ibv strains are restricted to replication in ex vivo organ cultures or in ovo and do not replicate in cell culture, making the study of their basic virology difficult. entry of ibv into cells is facilitated by the large glycoprotein on the surface of the virion, the spike (s) protein, comprised of s1 and s2 subunits. previous researc ... | 2020 | 33003350 |
| development of a recombinant thermostable newcastle disease virus (ndv) vaccine express infectious bronchitis virus (ibv) multiple epitopes for protecting against ibv and ndv challenges. | newcastle disease (nd) and infectious bronchitis (ib) are two highly contagious diseases that severely threaten the poultry industry. the goal of this study is to prevent these two diseases and reduce the vaccine costs during storage and transportation. in this study, we design a thermostable recombinant newcastle disease virus (ndv) candidate live vaccine strain designated as rls-t-hn-t/b, which expresses the multiple epitope cassette of the identified infectious bronchitis virus (ibv) (s-t/b). ... | 2020 | 33019497 |
| phylogenetic analysis of infectious bronchitis virus circulating in southern china in 2016-2017 and evaluation of an attenuated strain as a vaccine candidate. | avian infectious bronchitis (ib) is a highly contagious viral respiratory disease, caused by infectious bronchitis virus (ibv), that poses an important economic threat to the poultry industry. in recent years, genotypes gi-7, gi-13, and gi-19 have been the most prevalent ibv strains in china. however, in this study, we found that most ibv strains from southern china in 2016-2017 belonged to genotype gvi-1. this genotype, for which there is no vaccine, has been reported sporadically in the region ... | 2020 | 33067648 |
| development and immunogenic potentials of chitosan-saponin encapsulated dna vaccine against avian infectious bronchitis coronavirus. | infectious bronchitis (ib) is an economically important disease of poultry that also serve as model for the understanding of other coronaviruses associated diseases. ib is considered as a major challenge to the poultry industry worldwide as a result of its effect on egg production, weight gain as well as mortality. different ibv genotypes continue to emerge, thus, the need for broad based vaccines to curb the disease. based on bioinformatic data obtained in this study, sets of monovalent (either ... | 2020 | 33068733 |
| viral variant visualizer (vvv): a novel bioinformatic tool for rapid and simple visualization of viral genetic diversity. | here a bioinformatic pipeline vvv has been developed to analyse viral populations in a given sample from next generation sequencing (ngs) data. to date, handling large amounts of data from ngs requires the expertise of bioinformaticians, both for data processing and result analysis. consequently, vvv was designed to help non-bioinformaticians to perform these tasks. by providing only the ngs data file, the developed pipeline generated consensus sequences and determined the composition of the vir ... | 2020 | 33080244 |
| coronavirus: proteomics analysis of chicken kidney tissue infected with variant 2 (is-1494)-like avian infectious bronchitis virus. | avian infectious bronchitis virus is one of the most important gammacoronaviruses, which causes a highly contagious disease. in this study, we investigated changes in the proteome of kidney tissue of specific-pathogen-free (spf) chickens that were infected with an isolate of the nephrotropic variant 2 genotype (is/1494/06) of avian coronavirus. twenty 1-day-old spf white leghorn chickens were randomly divided into two groups, each comprising 10 chickens, which were kept in separate positive-pres ... | 2020 | 33083914 |
| a label-free electrochemical assay for coronavirus ibv h120 strain quantification based on equivalent substitution effect and aunps-assisted signal amplification. | a label-free electrochemical strategy is proposed combining equivalent substitution effect with aunps-assisted signal amplification. according to the differences of s1 protein in various infectious bronchitis virus (ibv) strains, a target dna sequence that can specifically recognize h120 rna forming a dna-rna hybridized double-strand structure has been designed. then, the residual single-stranded target dna is hydrolyzed by s1 nuclease. therefore, the content of target dna becomes equal to the c ... | 2020 | 33094371 |
| novel cyclovirus identified in broiler chickens with transmissible viral proventriculitis in china. | in october 2018, an outbreak of transmissible viral proventriculitis (tvp) occurred in 30-day-old commercial broiler chickens on a farm in weifang, china. tvp, an infectious viral disease characterized by runting and stunting, is associated with many viruses, and has a significant economic impact on the global poultry industry. tvp is diagnosed according to clinical symptoms, gross and histological lesions, and negative pcr results for pathogenic bacteria, avian leukosis virus subgroup j, marek' ... | 2020 | 33134354 |
| genetic and antigenic heterogeneity of gi-1/massachusetts lineage infectious bronchitis virus variants recently isolated in china. | four gi-1/massachusetts-type (gi-1/mass-type) infectious bronchitis virus (ibv) strains were isolated and the complete genomes of these isolates, coupled with the mass-type live-attenuated vaccine h120 and the mass-type pathogenic m41 strains, were sequenced in the present study. our results show that isolates ljl/140820 and i0306/17 may be derived from the ma5 (another mass-type live-attenuated vaccine strain) and h120 vaccine strains, respectively. the i1124/16 strain was found to be a m41 var ... | 2020 | 33142461 |
| the chicken mhc: insights into genetic resistance, immunity, and inflammation following infectious bronchitis virus infections. | the chicken immune system has provided an immense contribution to basic immunology knowledge by establishing major landmarks and discoveries that defined concepts widely used today. one of many special features on chickens is the presence of a compact and simple major histocompatibility complex (mhc). despite its simplicity, the chicken mhc maintains the essential counterpart genes of the mammalian mhc, allowing for a strong association to be detected between the mhc and resistance or susceptibi ... | 2020 | 33147703 |
| comparative trachea transcriptome analysis between avian infectious bronchitis virus and avian pathogenic e. coli individual infection and co-infection in spf chickens. | avian infectious bronchitis virus (ibv) and avian pathogenic escherichia coli (apec) are two important respiratory pathogens in the chicken. the co-infection can lead to chronic complications and considerable economic losses in the poultry industry worldwide. in the present study, we compared differential transcriptional profiles in the trachea tissue of three infected groups (ibv, apec, and co-infection) with the control group to investigate transcriptome profile changes at the early stage of t ... | 2020 | 33151740 |
| the emergence of q1 genotype of avian infectious bronchitis virus in iran, 2019: the first report. | avian infectious bronchitis (ib) is an infectious viral disease of chickens. the effective protection of chickens against many different infectious bronchitis virus (ibv) variants is not achieved unless the circulating genotypes in the region are identified and the cross-protection of the potential of vaccines in use is assessed. | 2020 | 33178303 |
| a highly pathogenic recombinant infectious bronchitis virus with adaptability in cultured cells. | infectious bronchitis virus (ibv) of gi-19 (qx), gi-7 (tw), gi-13 (4/91) and gi-1 (mass) lineages have been frequently detected in china in recent years. here, an ibv strain, referred as gd17/04, was isolated from the dead yellow feather chicken vaccinated with h52 and 4/91 vaccines, whose genome sequence was obtained through high-throughput sequencing. then it has been confirmed by the rdp and simplot analysis that gd17/04 is a recombinant strain deriving from yx10, 4/91, tw 2575/98 and h52 str ... | 2020 | 33207263 |
| experimental study of forsythoside a on prevention and treatment of avian infectious bronchitis. | forsythoside a is the main active ingredient in the chinese medicine forsythia suspensa, which has antiviral, anti-inflammatory, antioxidation, and immunoregulatory effects. it is reported that forsythoside a can significantly inhibit the replication of the avian infectious bronchitis virus(ibv) in cells, but there is no report in chickens. the present study aimed to investigate the effect of forsythoside a on ibv-m41, experiments were designed using 120 chickens at 12 days of age. the chickens ... | 2020 | 33234322 |
| gammacoronavirus avian infectious bronchitis virus and alphacoronavirus porcine epidemic diarrhea virus exploit a cell-survival strategy via upregulation of cfos to promote viral replication. | coronaviruses have evolved a variety of strategies to optimize cellular microenvironment for efficient replication. in this study, we report the induction of ap-1 transcription factors by coronavirus infection based on genome-wide analyses of differentially expressed genes in cells infected with avian coronavirus infectious bronchitis virus (ibv). most members of the ap-1 transcription factors were subsequently found to be upregulated during the course of ibv and porcine epidemic diarrhea virus ... | 2020 | 33239458 |
| molecular epidemiology and genotyping of infectious bronchitis virus and avian metapneumovirus in backyard and commercial chickens in jimma zone, southwestern ethiopia. | poultry production plays a relevant role in the ethiopian economy and represents a source of poverty alleviation for several social classes. infectious diseases can therefore significantly impact the economy and welfare. despite infectious bronchitis virus (ibv) and avian metapneumovirus (ampv) being present, the knowledge of their epidemiology and impact is extremely limited. in the present work, a cross-sectional study based on 500 tracheal swabs collected from 50 intensive and backyard unvacc ... | 2020 | 33255570 |
| evaluation of the antigen relatedness and efficacy of a single vaccination with different infectious bronchitis virus strains against a challenge with malaysian variant and qx-like ibv strains. | the predominant infectious bronchitis virus (ibv) strains detected in chickens in malaysia are the malaysian variant (mv) and qx-like, which are associated with respiratory distress, nephropathy, and high mortality. on the other hand, the antigenic relatedness and efficacy of ibv vaccines against these 2 field ibv strains are not well characterized. | 2020 | 33263227 |
| pathological effect of infectious bronchitis disease virus on broiler chicken trachea and kidney tissues. | this study aimed to investigate the pathological effects of the infectious bronchitis virus (ibv) on chicken trachea and kidney tissues and also desired to diagnose the virus genome using a molecular tool. | 2020 | 33281357 |
| infectious bronchitis virus: identification of gallus gallus apn high-affinity ligands with antiviral effects. | infectious bronchitis virus (ibv) is a coronavirus, causes infectious bronchitis (ib) with high morbidity and mortality, and gives rise to huge economic losses for the poultry industry. aminopeptidase n (apn) may be one of the ibv functional receptors. in this study, gallus gallus apn (gapn) protein was screened by phage-displayed 12-mer peptide library. two high-affinity peptides h (hdylyytftgnp) and t (tkfsppsfwylh) to gapn protein were selected for in depth characterization of their anti-ibv ... | 2020 | 33340637 |
| effects of amino acid-bound zinc and manganese feed additives on mhc haplotype chickens challenged with infectious bronchitis coronavirus. | major histocompatibility complex (mhc) congenic chicken lines have been used as a model to study infectious bronchitis virus (ibv) immune responses in chickens. zinc (zn) and manganese (mn) are trace minerals that act as enzyme cofactors in cellular reactions. in addition, zn is an important modulator of immune responses, especially in the respiratory tract. zinc and zn + mn amino acid complex supplements were tested to alleviate the effects of an ibv challenge using relatively resistant and sus ... | 2020 | 33347551 |