Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| use of isotopically chiral [4'-13c]penciclovir and 13c nmr to determine the specificity and absolute configuration of penciclovir phosphate esters formed in hsv-1 and hsv-2 infected cells and by hsv-1-encoded thymidine kinase. | penciclovir is a potent antiherpesvirus agent which is highly selective due to its phosphorylation only in virus infected cells. phosphorylation of one of the hydroxymethyl groups of penciclovir (pcv) creates a chiral centre leading to the possible formation of (r)- and (s)-enantiomers. the absolute configuration and stereospecificity of the pcv-phosphates produced in cells infected with herpes simplex viruses types 1 and 2 (hsv-1 and hsv-2), as well as by hsv-1-encoded thymidine kinase, were de ... | 1993 | 8305285 |
| flow cytometric indirect immunofluorescence assay with high sensitivity and specificity for the detection of antibodies to hsv-1 and hsv-2. | cells infected with hsv-1 or hsv-2 develop viral antigens which can be detected by immunofluorescence. we developed a flow cytometric indirect immunofluorescence assay to detect and quantitate antibodies to hsv-1 and hsv-2 in human sera. results obtained by flow cytometry for detecting antibodies against hsv-1, when compared with results obtained by elisa, showed an index of overall agreement of 100%. the correlation between the antibody titers obtained with each method was found to be highly si ... | 1993 | 8307141 |
| cytomegalovirus/herpesvirus and carotid atherosclerosis: the aric study. | ubiquitous viruses such as members of the human herpes virus group, particularly cytomegalovirus (cmv), have been proposed to be clinically important agents in the initiation and progression of atherosclerosis. antibodies to cmv and herpes simplex virus type 1 (hsv1) and type 2 (hsv2) were determined in 340 matched case-control pairs from the atherosclerosis risk in communities (aric) study. cases were defined by b-mode ultrasonography as persons with thickened carotid artery walls consistent wi ... | 1994 | 8308517 |
| sexually transmitted agents and cervical neoplasia in colombia and spain. | case-control studies of cervical intra-epithelial neoplasia grade iii (cin iii) and of invasive cervical cancer were carried out in spain and colombia to assess the relationship between cervical cancer and 6 common sexually transmitted agents (stas). the cin-iii studies included 525 cases and 512 controls matched for age and for the place of recruitment; the invasive-cancer studies included 373 histologically confirmed cases of squamous-cell carcinoma and 387 age-stratified controls selected ran ... | 1994 | 8314322 |
| the herpes simplex virus type 2 ul3 open reading frame encodes a nuclear localizing phosphoprotein. | the herpes simplex virus type 2 (hsv-2, strain 333) ul3 open reading frame (orf) codes for a protein with a predicted molecular weight of 29,681. comparisons of the ul3, strain 333 orf show essentially complete amino acid identity with hsv-2 (strain hg52) and a 75% amino acid identity with hsv type 1 (strain 17). to characterize the expression of this gene, a hydrophilic region of the hsv-2 ul3 gene was cloned into a bacterial expression vector. the resulting fusion protein was used to generate ... | 1993 | 8337818 |
| detection of herpesviruses by polymerase chain reaction in lymphocytes from patients with rheumatoid arthritis. | to investigate the occurrence of herpesviruses, including epstein-barr virus (ebv), herpes simplex viruses types 1 and 2 (hsv-1; hsv-2), and human herpesvirus 6 (hhv-6), in lymphocytes from patients with rheumatoid arthritis (ra) of less than 1 year's duration. | 1993 | 8343184 |
| mucosal immunity and protection after intranasal immunization with recombinant adenovirus expressing herpes simplex virus glycoprotein b. | a recombinant adenovirus (ad) expressing glycoprotein b (gb) of herpes simplex virus (hsv) type 1 (adgb8) was evaluated as a mucosal vaccine candidate. when administered intranasally (inl) to c57b1/6 mice, adgb8 induced levels of serum anti-hsv gb igg antibodies similar to those of mice immunized intraperitoneally (ip), which neutralized both hsv-1 and -2. mice immunized inl with adgb8 produced secretory iga specific for hsv gb, but mice immunized ip did not. splenic anti-hsv cytotoxic t lymphoc ... | 1993 | 8354903 |
| human papillomaviruses and other influences on survival from cervical cancer in panama. | to determine the influence on survival from cervical cancer of human papillomaviruses (hpvs) and other factors including age, herpes simplex virus type 2 (hsv-2) antibody status, and number of pregnancies. | 1993 | 8380103 |
| [demonstration of herpes simplex virus (hsv)-2 igg and igm using elisa in transsexuals and homosexuals]. | in this study, the sera of 12 cases of transsexual with vaginoplasty and 15 cases of homosexual have been investigated with elisa from the view point of hsv-2 igg and igm antibodies. twenty-five cases were within the 18-26 age groups and the other 2 transsexuals were 36 years old. our studying group have no significant clinical pathology. in all the 27 cases of transsexual and homosexual have been found to be positive for hsv-2 igg. in 7 cases, hsv-2 igm have been found positive, the other 20 ca ... | 1993 | 8380622 |
| herpes laryngitis and tracheitis causing respiratory distress in a neonate. | a newborn presented 1 week after birth with the sudden onset of stridor and respiratory distress. at endoscopy, the supraglottic and subglottic tissues were edematous and erythematous, and numerous plaquelike ulcers were seen on the mucosal surface. cultures of the lesions yielded herpes simplex virus type 2. the infant required a prolonged course of acyclovir and remained intubated for 31 days, but ultimately had both virological and clinical cure. evaluation and further treatment of this unusu ... | 1993 | 8381288 |
| rapid detection of herpes simplex virus in culture by in situ hybridization. | detection of herpes simplex virus (hsv) by a newly developed hsv-dna in situ hybridization (ish) technique (diagnostic hybrids, inc., athens, oh, usa) was compared with a reference standard that combines observation for cytopathic effect in shell vial cultures with subsequent identification of virus by staining with fluorescein-labeled hsv-specific monoclonal antibody. the new technique utilizes a probe consisting of an alkaline phosphatase direct labeled, cloned, single stranded dna fragment th ... | 1993 | 8381792 |
| hla-unrestricted killing of hsv-1-infected mononuclear cells. involvement of either gamma/delta+ or alpha/beta+ human cytotoxic t lymphocytes. | the characterization of hsv-specific human ctl, obtained in short term cultures by stimulating pbmc of healthy hsv-immune donors with autologous, pha-activated, hsv-1-infected mononuclear cells, is described. ctl induced by using this technique are able to mediate a strong lytic activity against both hsv-1- and hsv-2-infected targets, whereas they do not kill autologous ebv-lymphoblastoid cell lines unless they are superinfected with hsv-1. tcr-gamma/delta+ cells are mainly responsible for hsv-s ... | 1993 | 8381835 |
| adjuvant effects of imiquimod on a herpes simplex virus type 2 glycoprotein vaccine in guinea pigs. | the adjuvant effects of imiquimod for a herpes simplex virus (hsv) vaccine were evaluated. guinea pigs were immunized with 35 micrograms of lectin-purified hsv-2 glycoproteins 14 and 35 days before intravaginal hsv-2 inoculation. immunizations were given either alone, with complete freund's adjuvant, or with three different 5-day regimens of imiquimod. although immunization alone decreased the severity of the acute disease and viral replication, in two separate experiments, the addition of imiqu ... | 1993 | 8382722 |
| herpes simplex virus infection of human fibroblasts and keratinocytes inhibits recognition by cloned cd8+ cytotoxic t lymphocytes. | cd8+ cytotoxic t lymphocytes (ctl) clones with specificity for herpes simplex virus (hsv) were derived from two donors with genital hsv-2 infection. these ctl clones specifically lysed hsv-infected autologous b lymphoblastoid cells, but not hsv-infected fibroblasts. exogenous peptide loading sensitized both cell types to lysis by an hsv-specific ctl clone of known specificity. hsv infection rendered fibroblasts refractory to peptide sensitization. hsv infection also rendered fibroblasts and kera ... | 1993 | 8383706 |
| risk of acquisition of genital herpes simplex virus type 2 in sex partners of persons with genital herpes: a prospective couple study. | in a prospective study of factors associated with genital herpes simplex virus (hsv) type 2 transmission in couples in whom 1 partner had clinical genital hsv and the other did not, 11 (19%) of the 57 history-negative partners had hsv-2 antibody by western blot at entry. in follow-up (mean, 16 months) of 29 hsv-2-seronegative partners, overall 4 (14%) seroconverted compared with 3 (23%) of 13 hsv-1- and -2-seronegative partners and 1 (6%) of 16 hsv-1-seropositive partners. since all seroconverte ... | 1993 | 8383724 |
| differential mechanism of cytostatic effect of (e)-5-(2-bromovinyl)-2'-deoxyuridine, 9-(1,3-dihydroxy-2-propoxymethyl)guanine, and other antiherpetic drugs on tumor cells transfected by the thymidine kinase gene of herpes simplex virus type 1 or type 2. | after they have been transfected with the herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) thymidine kinase (tk) gene murine mammary carcinoma (fm3a) cells become highly sensitive to the growth inhibitory properties of the antiherpetic agents (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu), 9(-)[(2-hydroxyethoxy)methyl]guanine (acyclovir, acv), 9(-)[(1,3-dihydroxy-2-propoxy)methyl]guanine (dhpg, ganciclovir), and 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-methyluracil (fmau). bvdu was 100-f ... | 1993 | 8384209 |
| the pseudorabies virus host-shutoff homolog gene: nucleotide sequence and comparison with alphaherpesvirus protein counterparts. | the virion host shutoff function (vhs) of many herpesviruses is required for inhibition of cellular gene expression in infected cells. this function corresponds to the ul41 open reading frame of herpes simplex virus type 1 (hsv-1) and homolog sequences have been found in other alphaherpesvirus, like hsv-2, varicella-zoster virus (vzv) and equine herpesvirus type 1 (ehv-1). in this work, we have cloned and sequenced a pseudorabies virus (prv) gene which is homologous to the vhs genes of the other ... | 1993 | 8384744 |
| antiviral effect of oligo(nucleoside methylphosphonates) complementary to the herpes simplex virus type 1 immediate early mrnas 4 and 5. | we have previously shown that an oligo(nucleoside methylphosphonate) (deoxynucleoside methylphosphonate residues in italics) complementary to the acceptor splice junction of herpes simplex virus type 1 (hsv-1) immediate-early (ie) pre-mrnas 4,5 [d(tptcctcctgcgg)], causes sequence-specific inhibition of virus growth in infected cell cultures (smith et al., 1986; kulka et al., 1989). here we report a similar inhibition of hsv-1 growth by oligo(nucleoside methylphosphonates) complementary to the sp ... | 1993 | 8384823 |
| encephalitis in immunocompetent patients due to herpes simplex virus type 1 or 2 as determined by type-specific polymerase chain reaction and antibody assays of cerebrospinal fluid. | a herpes simplex virus type 2 (hsv 2) etiology was sought in 93 consecutive cases of herpes simplex encephalitis (hse) in immunocompetent post neonate patients. antibodies to hsv 2 glycoprotein g antigen were determined by an enzyme-linked immunosorbent assay (elisa) and hsv 2 dna in cerebrospinal fluid (csf) by a nested polymerase chain reaction (pcr) assay with primer pairs in the glycoprotein g gene. evidence of hsv 2 infection was found in 6 patients; hsv 2 dna was demonstrated in csf and th ... | 1993 | 8385702 |
| baculovirus-expressed herpes simplex virus type 2 glycoprotein d is immunogenic and protective against lethal hsv challenge. | herpes simplex virus type 2 glycoprotein d (gd2) was cloned and expressed in the baculovirus-spodoptera frugiperda system. milligram quantities of glycoprotein were recovered from suspension culture and subjected to purification by ion-exchange and immunoaffinity chromatography. the resultant purified gd existed as a homogeneous 57,500 mw monomeric species demonstrating reactivity with anti-gd monoclonal antibodies including those directed at a non-sequential neutralizing epitope of gd. immuniza ... | 1993 | 8385842 |
| sequence analysis of herpes simplex virus gb gene homologs of two platyrrhine monkey alpha-herpesviruses. | homologs of the herpes simplex virus gb gene were identified in two alpha-herpesviruses of platyrrhine monkeys, herpesvirus saimiri 1 (hvs 1) and h. ateles 1 (hva 1). these genes were cloned and sequenced in their entirety. analysis of the predicted amino acid sequences indicated that the gb glycoproteins of these two viruses are of similar size, have 10 cys residues and 5 potential n-linked glycosylation sites which align exactly with those in other primate alpha-herpesvirus gb polypeptides, an ... | 1993 | 8385913 |
| nucleotide sequence of the major dna-binding protein gene of herpes simplex virus type 2 and a comparison with the type 1. | the nucleotide sequence of a region encompassing about 5,200 base pairs (bp) of the left side of the origin of replication in the long unique region of the herpes simplex virus type 2 (hsv-2) has been determined. this region contained the major dna-binding protein or the infected-cell protein 8 (icp 8) gene and 5'-part of the counterpart of hsv-1 icp 18.5 gene. a comparison of the nucleotide sequence of the icp8 gene between hsv-1 and hsv-2 showed an 89.8% homology. a primer extension analysis f ... | 1993 | 8385914 |
| analysis of a herpes simplex virus 2 fragment from the open reading frame of the large subunit of ribonucleotide reductase with transcriptional regulatory activity. | expression of herpes simplex virus 2 (hsv-2)-encoded ribonucleotide reductase (rr) is required for growth in nondividing cells. the functional enzyme is composed of a large and a small subunit. in virus-infected cells, rr is expressed temporally as a delayed early protein. however, the promoter regulatory region of the large subunit can function as an immediate early promoter in transient transfection assays, suggesting that expression may be quite complex. in this study, a 95-bp fragment derive ... | 1993 | 8385955 |
| human papillomavirus, herpes simplex virus and other potential risk factors for cervical cancer in a high-risk area (greenland) and a low-risk area (denmark)--a second look. | the prevalence of human papillomavirus (hpv) infection and other risk factors were studied in a high risk area for cervical cancer (greenland) and in a low risk area (denmark). from nuuk (greenland) and nykøbing falster (denmark), random samples of 150 women aged 20-39 years were drawn. a total of 129 and 126 women were included in greenland and denmark, respectively. the proportion of hpv infected women assessed by virapap was similar in denmark and greenland (4.8 vs 3.9%). when type specific p ... | 1993 | 8385982 |
| antiviral properties of the seed extract of an indian medicinal plant, pongamia pinnata, linn., against herpes simplex viruses: in-vitro studies on vero cells. | pongamia pinnata, linn., an indian medicinal plant used in the ayurvedha and siddha traditional medicine systems, for treatment of clinical lesions of skin and genitalia, was evaluated for antiviral properties against herpes simplex virus type-1 (hsv-1) and type-2 (hsv-2) by in-vitro studies in vero cells. a crude aqueous seed extract of p. pinnata completely inhibited the growth of hsv-1 and hsv-2 at concentrations of 1 and 20 mg/ml (w/v), respectively, as shown by complete absence of cytopathi ... | 1993 | 8386251 |
| resistance of herpes simplex virus type 2 to neomycin maps to the n-terminal portion of glycoprotein c. | entry of herpes simplex virus (hsv) into cells is believed to be mediated by specific binding of envelope proteins to a cellular receptor. neomycin specifically blocks this initial step in infection by hsv-1 but not hsv-2. resistance of hsv-2 to this compound maps to a region of the genome encoding glycoprotein c (gc-2). we have studied the function of gc-2 in the initial interaction of the virus with the host cell, using hsv-2 mutants deleted for gc-2 and gc-2-rescued recombinants. resistance t ... | 1993 | 8386261 |
| orientation of the cleavage site of the herpes simplex virus glycoprotein g-2. | during the synthesis of glycoprotein g-2 (gg-2) of herpes simplex virus type 2, the 104,000-da gg-2 precursor (104k precursor) is cleaved to generate the 72k and the 31k intermediates. the 72k product is processed to generate the mature gg-2 (molecular mass, 108,000 da), while the 31k product is additionally processed and secreted into the extracellular medium as the 34k component (h. k. su, r. eberle, and r. j. courtney, j. virol. 61:1735-1737, 1987). in this study, the orientations of the 31k ... | 1993 | 8386284 |
| the proportion of herpes simplex virus-specific cytotoxic t lymphocytes (tc) that recognize glycoprotein c varies between individual mice and is dependent on the form of immunization. | in mice the immune response to hsv-1 includes a brisk tc response that is intimately associated with the control of infection. this report evaluated the tc response to gc, one of the envelope glycoproteins of hsv-1. this protein was recognized as a target antigen for tc from hsv-1 immune mice only if they expressed the h-2kb mhc allele. however, even within these "responder" strains of mice the proportion of gc specific tc was highly variable. the failure of hsv-induced tc to recognize gc in the ... | 1993 | 8386515 |
| efficacy of the herpes simplex virus types 1 and 2 mutant viruses to confer protection against zosteriform spread in mice. | two mutant viruses, hsv-2 xd192 and hsv-1 1716, failed to generate zosteriform lesions when injected in high dose into balb/c and c3h mice. mice exposed to mutant viruses were solidly immune to challenge by wild-type homologous or heterologous virus. however, at lower immunizing doses protection was evident against lethality, but not skin lesions, especially in the case of mutant xd192. protection could be conferred with lymphoid cells from mutant virus immune mice and again, protection against ... | 1993 | 8386516 |
| herpes simplex virus type-2 infection by a footpad route results in neuronal death in mouse spinal ganglia. | we have examined the effects of herpes simplex virus type 2 (hsv-2) infection on neuron numbers in mouse dorsal root ganglia (drg) following unilateral hind footpad inoculation. one month following hsv-2 strain ms inoculation, tissue sections of decalcified spine containing the paired 4th and 5th lumbar drgs were stained with cresyl violet. neuronal numbers, somal areas and ganglion volumes were determined for ganglia ipsilateral and contralateral to both hsv-2 and medium inoculations. one month ... | 1993 | 8387099 |
| in vitro antiviral activity of polyoxomolybdates. mechanism of inhibitory effect of pm-104 (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o on human immunodeficiency virus type 1. | a screening for inhibitors of human immunodeficiency virus type 1 (hiv-1) among various types of isopolyoxomolybdates and heteropolyoxomolybdates was carried out by using an in vitro assay system measuring the cytopathogenicity of hiv-1 in cd4+ human mt-4 cells. a novel heteropolyoxomolybdate named pm-104 with the chemical formula (nh4)12h2(eu4(moo4)(h2o)16(mo7o24)4).13h2o was found to be associated with potent anti-hiv-1 activity. pm-104 interferes with virus infection at a very early step such ... | 1993 | 8387260 |
| induction and enhancement of immune responses to herpes simplex virus type 2 in humans by use of a recombinant glycoprotein d vaccine. | a vaccine for a chronic or recurrent viral infection should induce immune responses that protect against primary disease or that augment preexisting defenses sufficiently to diminish the likelihood of disease recurrence or progression. such a vaccine was sought for genital herpes, a sexually transmitted infection of epidemic proportion. vaccine containing recombinant herpes simplex virus type 2 glycoprotein d expressed in cho cells was given repeatedly and safely to 24 human volunteers. in previ ... | 1993 | 8387560 |
| synthesis and antiviral activity of novel isonucleoside analogs. | a series of branched-chain sugar isonucleosides was synthesized and evaluated for antiviral activity against herpesviruses. the preparation of homochiral [3s-(3 alpha, 4 beta, 5 alpha)]-2-amino-1, 9-dihydro-9-[tetrahydro-4,5-bis(hydroxymethyl)-3-furanyl]-6h-purin-6-one (7, bms-181,164) and related compounds was stereospecifically achieved starting from 1,2-isopropylidene-d-xylofuranose (10). an efficient two-step reduction of the anomeric center of bis-acetate 18 involved formation of the chlori ... | 1993 | 8387600 |
| herpes simplex virus type 2 antibody in patients attending antenatal or std clinics. | to determine the prevalence of antibody to herpes simplex virus type 2 (hsv-2) in patients attending a general public antenatal clinic and three public sexually transmitted disease (std) clinics in sydney. | 1993 | 8387628 |
| kinetic studies of the predicted substrate-binding site of varicella-zoster virus thymidine kinase. | to investigate the mechanism of kinetic action and substrate recognition of varicella-zoster virus (vzv) thymidine kinase (tk), we designed and isolated a site-directed mutant vzv tk which has double amino acid substitutions, 136threonine to leucine and 137isoleucine to leucine (sdm tk). this mutant was designed to alter the substrate-binding site of the vzv tk to duplicate that of the herpes simplex virus type 2 enzyme. kinetic studies of the activity of wild-type tk indicated that the binding ... | 1993 | 8389797 |
| characterization of human keratinocytes transformed by high risk human papillomavirus types 16 or 18 and herpes simplex virus type 2. | recent reports implicate two dna tumour viruses, herpes simplex virus type 2 (hsv-2) and human papillomavirus types 16 or 18 (hpv-16 and -18), in the pathogenesis of cervical cancer. previous studies have indicated that primary human fibroblasts transfected with hpv-16 and hsv-2 morphological transforming region iii (mtr iii) are more aneuploid than fibroblasts immortalized with hpv-16 and that hsv-2 dna sequences are retained in transformed cells. since hsv-2 and hpv typically infect cells of e ... | 1993 | 8389810 |
| guanylylation and adenylylation of the alpha regulatory proteins of herpes simplex virus require a viral beta or gamma function. | herpes simplex virus genes form several groups whose expression is coordinately regulated and sequentially ordered in a cascade fashion. most of the products of the first group, the alpha genes, appear to have regulatory functions. we report that the alpha proteins, infected cell proteins 4, 0, 22, and 27 of herpes simplex virus 1 and 4, 0, and 27 of herpes simplex virus 2, were labeled in the isolated nuclei of infected hela cells with [alpha-32p]gtp or [alpha-32p]atp late in infection and that ... | 1993 | 8389911 |
| herpes simplex virus infection in pregnancy. | genital infections in pregnancy caused by herpes simplex virus types 1 and 2 are a difficult management problem. both primary and recurrent genital herpes can range from severe, extensive ulceration to asymptomatic virus shedding. although neonatal herpes is a well recognized complication of symptomatic maternal primary genital infection at the time of delivery, most cases are associated with asymptomatic virus shedding and absence of a history of genital herpes. neonatal herpes may also be acqu ... | 1993 | 8390339 |
| oral acyclovir and recurrent genital herpes during late pregnancy. | to assess plasma acyclovir levels in pregnant women given oral acyclovir during late gestation and to determine the role and effect of oral acyclovir on asymptomatic shedding of virus in cases of recurrent genital herpes. | 1993 | 8390630 |
| herpesvirus infections of the cns: mr findings. | herpesvirus infections of the cns are prevalent in all segments of the population, and are an important health care issue. the family of herpesviruses consists of a large group of double-stranded dna viruses that includes herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), cytomegalovirus, epstein-barr virus, varicella-zoster virus, b virus, herpesvirus 6, and herpesvirus 7. in addition to producing infection when the host initially acquires the virus, an important property ... | 1993 | 8390790 |
| the characteristic which makes the cell-coded hsv-inducible u90 distinctive in transformed cells is its greatly increased half-life. | a set of polypeptides detected in transformed cells of m(r) values 90,000 (a doublet) 40,000 and 32,000 that are recognised by immunoprecipitation of l-[35s]methionine-labelled tumour cell lysates, with sera from tumour-bearing rats and with antisera raised against herpes simplex virus type 2 (hsv-2)-infected cells, were previously reported (macnab et al., 1985, 1992; hewitt et al., 1991). these proteins are cell-coded and cannot be precipitated from similarly radiolabelled control cells. two of ... | 1993 | 8391013 |
| phosphorylation of neurotropic alphaherpesvirus envelope glycoproteins: herpes simplex virus type 2 ge2 and pseudorabies virus gi. | i have examined the state of phosphorylation of the envelope glycoproteins of two neurotropic herpesviruses, hsv-2 and prv. hsv-2 ge2 and prv gi, which are the homologues to hsv-1 ge, were found to be phosphorylated and phosphoaminoacid analysis revealed that both contained phosphoserine. these findings are consistent with a conserved phosphorylation of the glycoprotein homologues to hsv ge among the neurotropic alphaherpesviruses. | 1993 | 8391184 |
| evaluation of hpmpc therapy for primary and recurrent genital herpes in mice and guinea pigs. | the nucleoside analogue (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (hpmpc) inhibited the replication of herpes simplex virus (hsv) types 1 and 2 in tissue culture cells at about 1.0 micrograms/ml, whereas acyclovir (acv) had an ec50 of about 0.10-0.50 micrograms/ml. the purpose of these studies was to evaluate the efficacy of topically applied hpmpc in animal models of primary and recurrent genital hsv-2 infections. mice treated with 5%, 1% or 0.5% hpmpc three times daily, beginning 6 o ... | 1993 | 8391249 |
| passive transfer of anti-herpes simplex virus type 2 monoclonal and polyclonal antibodies protect against herpes simplex virus type 1-induced but not herpes simplex virus type 2-induced stromal keratitis. | to investigate whether passive transfer of antibodies to viral glycoproteins would protect against herpes simplex virus type 2-induced stromal keratitis. | 1993 | 8392037 |
| molecular analysis of a neurovirulent herpes simplex virus type 2 strain with reduced thymidine kinase activity. | thymidine kinase (tk) of herpes simplex virus (hsv) has been identified as one of the factors responsible for its virulence. we have previously isolated acyclovir (acv)-resistant hsv type 2 (hsv-2), strain ys-4 c-1, by simple plaque cloning from a clinical isolate. although ys-4 c-1 had extremely low tk activity, it retained high virulence in mice. to determine the mechanism of the reduction of tk activity, a molecular analysis of the ys-4 c-1 tk gene was performed. ys-4 c-1 produced tk mrna, wh ... | 1993 | 8392322 |
| differentiation of herpes simplex virus types 1 and 2 by plaque appearances on semicontinuous rabbit lens epithelial cells in the clinical laboratory. | herpes simplex virus type 1 (hsv1) and type 2 (hsv2) were differentiated on the basis of different plaque appearance on semicontinuous rabbit lens epithelial (rle) cells. plaques produced by hsv1 strains were small; the mean diameter was 1.29 +/- 0.37 mm 3 days after inoculation. hsv2 strains produced large and small plaques, with the ratio of large to small about 20:1. the mean diameters of the large and the small plaques of hsv2 were 3.34 +/- 0.56 mm and 0.97 +/- 0.31 mm respectively 3 days af ... | 1993 | 8393061 |
| s-adenosylmethionine metabolism in herpes simplex virus type 2-infected cells. | infection of primary rat embryo cells with herpes simplex virus type 2 has previously been reported to produce a dramatic and rapid inhibition of cellular dna methylation. however, it has neither an immediate effect on s-adenosylmethionine breakdown nor on the relative pool sizes of s-adenosylmethionine and s-adenosylhomocysteine. | 1993 | 8393082 |
| synthesis and antiherpes virus activity of 1,5-anhydrohexitol nucleosides. | the synthesis of 1,5-anhydrohexitol nucleosides is described. these nucleoside analogues were obtained by alkylation of the heterocyclic bases with the tosylate 10 or by alkylation of the bases with the alcohol 12 under mitsunobu conditions. the compounds were evaluated for antiviral and cytostatic activity. highly selective activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) was noted for 1,5-anhydro-2,3-dideoxy-2-(5-iodouracil-1-yl)-d-arabino-hexitol 4b at a concentration o ... | 1993 | 8393114 |
| purification and characterization of the herpes simplex virus type 2-encoded uracil-dna glycosylase. | the herpes simplex virus type 2 (hsv-2) uracil-dna glycosylase (ung) was from the nuclei of infected kb cells using ion exchange, affinity, and chromatofocusing chromatography techniques. chromatography on dna cellulose revealed two distinct hsv-2-encoded ungs. one species, designated a, was purified approximately 324-fold, while the second species, designated b, was purified approximately 130-fold. the hsv ung species b was observed to unidirectionally convert to the a species, suggesting that ... | 1993 | 8393229 |
| transcriptional analysis of the herpes simplex virus type 1 region containing the trl/ul junction. | in this study we have used a combination of northern blot, cdna, and rnase protection analysis to characterize transcription occurring at the junction of the long terminal repeat (trl) and long unique segment (ul) of herpes simplex virus type 1 (hsv-1). two low abundance 5' colinear transcripts mapped between the junction of the trl/ul and the cap site of the primary latency associated transcripts (lat). analysis of the region containing the first four open translational reading frames (orfs) of ... | 1993 | 8395115 |
| inhibition of viral growth by an alpha-oligonucleotide directed to the splice junction of herpes simplex virus type-1 immediate-early pre-mrna species 22 and 47. | a 13-residue alpha-anomeric oligonucleotide [alpha-5'-d(gggcgtcctcctt)3'], 5'-substituted with a psoralen derivative, pso-alpha-13 psoralen linked to the 5' end of an alpha-anomeric n-residue oligonucleotide, was targeted to the acceptor splice junction of herpes simplex virus type-1 immediate-early pre-mrna species 22 and 47. inhibition of viral growth was observed upon irradiation of vero cells infected with herpes simplex virus type-1 and treatment with pso-alpha-13. the virus titer was decre ... | 1993 | 8396028 |
| the reliability of serological tests for the diagnosis of genital herpes: a critique. | the reliability and limitations of the currently used routine tests for herpes simplex virus type 2 (hsv-2) antibody in australia are reviewed. six case reports illustrate the clinical dangers of overinterpretation of the currently available kits and the need for a readily available specific hsv-2 antibody test. in sydney, hsv-2 causes approximately 85% of primary genital herpes and > 95% of recurrent genital herpes. due to the extensive serological cross-reactivity between hsv-1 and hsv-2, curr ... | 1993 | 8396232 |
| the rr1 gene of herpes simplex virus type 1 is uniquely trans activated by icp0 during infection. | as has been demonstrated for herpes simplex virus type 2, we show in this report that the herpes simplex virus type 1 ribonucleotide reductase large subunit (rr1) gene is trans activated in transient transfection assays by vp16 and icp0 but not by icp4. deletion analysis demonstrated that responsiveness to induction to vp16 resides in an octamer/taatgarat sequence of the rr1 promoter and that the tata box alone is sufficient to provide induction by icp0. the induction of the rr1 gene by icp0 but ... | 1993 | 8396674 |
| the effect of cytoplasmic domain mutations on membrane anchoring and glycoprotein processing of herpes simplex virus type 1 glycoprotein c. | the predicted amino acid sequence of herpes simplex virus type 1 glycoprotein c (hsv-1 gc) shows that it has the features of a typical type 1 integral membrane protein: a cleavable n-terminal signal sequence, a glycosylated ectodomain, a single transmembrane domain, and a small, charged cytoplasmic domain. in an earlier investigation of the function of the gc cytoplasmic domain, it was shown that the gc synthesized by a gc mutant, dl2, which lacked the last three residues of the transmembrane do ... | 1993 | 8396808 |
| nonoxynol-9 protects mice against vaginal transmission of genital herpes infections. | a vaginal application of a commercially available contraceptive jelly containing nonoxynol-9 (n9) prevented vaginal transmission of herpes simplex virus type 2 (hsv-2) infections in the mouse. when n9 jelly was delivered to the vagina with the virus inoculum, 20 s before inoculum, or 5 min before inoculum, mice were completely protected from visible infection (p < .001). protection lasted for at least 30 min (p < .03), and significant protection occurred even when the n9 jelly was delivered 15 m ... | 1993 | 8397261 |
| effect of indomethacin on ultraviolet radiation-induced recurrent herpes simplex virus disease in guinea-pigs. | exposure to u.v. radiation increases the local level of prostaglandins which may play a role in u.v. radiation-induced herpes simplex virus (hsv) recurrences. we used the guinea-pig model of u.v. radiation-induced recurrent genital hsv-2 disease for examining the effects of indomethacin, a prostaglandin inhibitor, on u.v.-induced recurrences. in the first experiment, performed 100 days after hsv-2 inoculation, treatment with indomethacin for 5 days begun 24 h before u.v.-irradiation decreased th ... | 1993 | 8397285 |
| [hevizos ointment efficacy in view of molecular pharmacology]. | the antiviral effect of 5-isopropyl-2'-deoxyuridine (ipdu) on herpes viruses (hsv-1 and hsv-2) is based on the sensitivity of virus dna to nucleases due to ipdu incorporation into the virus dna and the resulting change in dna conformation. virus selectivity is based on virus induced thymidine kinase activity and on hsv induction of polymerase enzymes catalyzing the incorporation. hevizos ointment containing ipdu has the following advantageous pharmacokinetic properties: high accumulation in the ... | 1993 | 8397468 |
| herpes simplex virus glycoprotein c: molecular mimicry of complement regulatory proteins by a viral protein. | herpes simplex virus (hsv) encodes a protein, glycoprotein c (gc), which binds to the third complement component, the central mediator of complement activation. in this study the structural and functional relationships of gc from hsv type 1 (hsv-1) and known human complement regulatory proteins factor h, properdin, factor b, complement receptor 1 (cr1) and 2 (cr2) were investigated. the interaction of gc with c3b was studied using purified complement components, synthetic peptides, antisera agai ... | 1993 | 8406590 |
| evaluation of a monoclonal antibody-based enzyme immunoassay for early detection of herpes simplex virus genital infection. | a monoclonal antibody-based enzyme immunoassay test for detection of herpes simplex virus (hsv) type-common antigen was evaluated in 40 women with vulvar lesion suspicious for genital hsv infection. the assay gave interpretable readings in 90% of the cases, with sensitivity and specificity of 100% and 92% respectively, a negative predictive value of 100%, and a positive predictive value of 75%. with a few limitations, the assay may be used for early diagnosis of genital hsv infection. | 1993 | 8407269 |
| an evaluation of oral ulcers in patients with aids and aids-related complex. | patients with hiv infection can have recurrent and persistent oral ulcers, not attributable to known infectious agents. | 1993 | 8408791 |
| autocrine secretion of interferon-alpha/beta and tumour necrosis factor-alpha synergistically activates mouse macrophages after infection with herpes simplex virus type 2. | resistance of mice to infection with herpes simplex virus type 2 (hsv-2) is strongly dependent on the function of macrophages (m phi). infection of mouse m phi with hsv-2 results in an early (4 to 10 h) activation of the cells with an enhanced respiratory burst generated after membrane triggering with a phorbol ester. the role of monokines produced during this infection was analysed. both interferon-alpha/beta (ifn-alpha/beta) and tumour necrosis factor-alpha (tnf-alpha) were produced within the ... | 1993 | 8409942 |
| [from herpes to zona]. | 1993 | 8410632 | |
| mollaret's meningitis associated with herpes simplex type 2 infection. | we describe three patients with benign recurrent aseptic meningitis (mollaret's meningitis). for one of these cases, the episodes of meningitis were associated with herpetic outbreaks. mollaret cells, which are a hallmark of mollaret's meningitis, were present in the csf from two of the three patients. in all cases, herpes simplex virus type 2 dna was present in the csf during the acute illness as detected by polymerase chain reaction amplification, although viral cultures from csf were all nega ... | 1993 | 8414021 |
| antiviral agents: characteristic activity spectrum depending on the molecular target with which they interact. | the target protein (enzyme) with which antiviral agents interact determines their antiviral activity spectrum. based on their activity spectrum, antiviral compounds could be divided into the following classes: (1) sulfated polysaccharides (i.e., dextran sulfate), which interact with the viral envelope glycoproteins and are inhibitory to a broad variety of enveloped viruses (i.e., retro-, herpes-, rhabdo-, and arenaviruses): (2) sah hydrolase inhibitors (i.e., neplanocin a derivatives), which are ... | 1993 | 8430518 |
| [clinical and pharmacologic review of antiviral agents]. | the author presents a review if pharmacokinetics, undesirable effects, drug interactions, dosage, indications and drug forms of antiviral chemotherapeutic agents used for systemic treatment: amantadine and rimantadine (influenza virus a), acyclovir (hsv-1, hsv-2 and vzv), ganciclovir (cmv), zidovudine (hiv), ribavirine (broad spectrum) and fosfocarnet (herpes viruses and hiv), mentioning also vidarabine. all mentioned preparations are at least in some countries commercially available. the author ... | 1993 | 8435845 |
| clinical reactivation of herpes simplex virus type 2 infection in seropositive pregnant women with no history of genital herpes. | to determine the risk for genital herpes and asymptomatic herpes simplex virus (hsv) shedding in late pregnancy and delivery in a population of hsv type 2 (hsv-2)-seropositive but previously asymptomatic pregnant women. | 1993 | 8439114 |
| serum antibodies and subsequent cervical neoplasms: a prospective study with 12 years of follow-up. | approximately 40,000 blood samples drawn in 1968-1972 by the social insurance institution's mobile clinic in finland have been stored. linking cancer incidence data obtained from the finnish cancer registry with this material yielded 32 cases of cervical cancer diagnosed after drawing of the blood sample. these 32 individuals and 64 matched controls were analyzed for serum antibodies indicative of past infection with chlamydia trachomatis, neisseria gonorrhoeae, cytomegalovirus, epstein-barr vir ... | 1993 | 8452120 |
| novel acyclonucleotides: synthesis and antiviral activity of alkenylphosphonic acid derivatives of purines and a pyrimidine. | a series of phosphonoalkenyl and (phosphonoalkenyl)oxy derivatives of purines and a pyrimidine were synthesized. these compounds are the first reported acyclonucleotides which incorporate the alpha,beta-unsaturated phosphonic acid moiety as the phosphate mimic and include compounds in which the acyclic substituent is attached to n-9 of a purine or n-1 of a pyrimidine by either a nitrogen-carbon or a nitrogen-oxygen bond. the phosphonoalkenyl-substituted compounds 7a-c, 8a-c, 9, 10, and 12 were p ... | 1993 | 8496903 |
| famciclovir. a review of its pharmacological properties and therapeutic efficacy in herpesvirus infections. | famciclovir, a synthetic acyclic guanine derivative, is a prodrug which, after oral administration, is rapidly metabolised to the highly bioavailable antiviral compound penciclovir. penciclovir is active in vitro against the herpesviruses herpes simplex virus (hsv)-1, hsv-2 and varicella zoster virus (vzv). famciclovir is an effective treatment of immunocompetent patients with acute herpes zoster (shingles) caused by vzv. comparative studies have demonstrated that famciclovir has therapeutic eff ... | 1995 | 8521764 |
| differences in the role of glycoprotein c of hsv-1 and hsv-2 in viral binding may contribute to serotype differences in cell tropism. | heparan sulfate serves as a receptor for several herpesviruses. for herpes simplex virus 1 (hsv-1), pseudorabies virus, and bovine herpesvirus 1, glycoprotein c homologues have been shown to mediate the binding to cell-surface heparan sulfate. it has been assumed that glycoprotein c of hsv-2 (gc-2) plays a similar role in hsv-2 entry, but this has not been established experimentally. we first determined, using heparin-affinity chromatography, that gc-2 is a heparin-binding glycoprotein. to exami ... | 1995 | 8525631 |
| effect of antibody alone and combined with acyclovir on neonatal herpes simplex virus infection in guinea pigs. | neonatal herpes simplex virus (hsv) infection produces severe disease with unacceptable morbidity and mortality with current antiviral therapies. the effect of therapy with passive anti-hsv antibody and acyclovir was evaluated using a guinea pig model of neonatal hsv. newborn animals were inoculated intranasally and treated with acyclovir (60 mg/kg/day) or antibody (or both), beginning on days 0, 2, or 3 after hsv-2 inoculation. acyclovir alone was effective only when begun on day 0, and antibod ... | 1996 | 8537645 |
| decline of herpes simplex virus type 2 and chlamydia trachomatis infections from 1970 to 1993 indicated by a similar change in antibody pattern. | antibodies to herpes simplex virus type 2 (hsv-2) and chlamydia trachomatis (ct) were determined in sera from pregnant women from 1970 and at intervals up to 1993. the trends for hsv-2 and ct infections were deduced from the observed antibody rates in different age groups during the observation period. total antibody rates for hsv-2 tended to decline toward the end of the period when age-matched groups were compared, while the ct antibody rates peaked in 1979 and then declined gradually. age-spe ... | 1995 | 8539540 |
| the ribonucleotide reductase inhibitor (e)-2'-fluoromethylene-2'-deoxycytidine (mdl 101,731): a potential topical therapy for herpes simplex virus infection. | the ribonucleotide reductase inhibitor mdl 101,731 was examined for antiviral activity against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) in vitro and in combination with acyclovir in the murine zosteriform model of hsv-1 infection. the in vitro antiviral activity (ic50) for both serotypes of hsv was similar and in the range 23-98 nm for vero cells. comparable activities were obtained against acyclovir-resistant viruses. in the zosteriform model, topical combination therapy of mdl 10 ... | 1995 | 8540753 |
| herpes simplex virus latency in tissue culture cells. | we have developed tissue culture systems which mimic certain aspects of herpes simplex virus (hsv) latency, in order to facilitate molecular studies of the phenomenon. the initial approach utilised incubation at 42 degrees c to suppress virus replication and establish stable latency after infection of human fibroblasts at low multiplicity with hsv-2. latent virus was reactivated efficiently by superinfection of cultures with hsv-1 or human cytomegalovirus. improvement of the system was achieved ... | 1994 | 8542396 |
| evidence of type 2 herpes simplex infection in human coronary arteries at the time of coronary artery bypass surgery. | to examine histologically biopsies from the coronary arteries of patients undergoing coronary artery bypass grafting (cabg) for evidence of herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) antigen and to correlate the incidence with pathological and clinical data. | 1995 | 8542544 |
| herpes simplex virus resistance to acyclovir: clinical relevance. | herpes simplex virus (hsv) infections are very common in the general population and can be treated with the nucleoside analogue acyclovir. acyclovir is initially phosphorylated intracellularly in hsv-infected cells by a viral-specific thymidine kinase to acyclovir-monophosphate. the monophosphate is subsequently di- and triphosphorylated by host cellular kinases to the active form of the drug, which inhibits hsv dna polymerase and incorporates into the elongating viral dna and causes chain termi ... | 1995 | 8548189 |
| [determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions]. | the effects of different experimental conditions on the antiviral activity of the sulphated xylomannan f6, isolated from the red seaweed nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (hsv-1 and hsv-2) were studied. the compound was equally effective against several strains of hsv-1 (f, kos, b-2006 and field) and hsv-2 (g) in different cell lines (vero, hep-2 and bhk-21). furthermore, the antiviral activity of f6 was independent of the method employed to dete ... | 1995 | 8552762 |
| synthesis and antiviral activity of 2'-deoxy-4'-thio purine nucleosides. | a series of 2'-deoxy-4'-thioribo purine nucleosides was prepared by trans-n-deoxyribosylase-catalyzed reaction of 2'-deoxy-4'-thiouridine with a variety of purine bases. this synthetic procedure is an improvement over methods previously used to prepare purine 4'-thio nucleosides. the compounds were tested against hepatitis b virus (hbv), human cytomegalovirus (hcmv), herpes simplex virus (hsv-1 and hsv-2), varicella zoster virus (vzv), and human immunodeficiency virus (hiv-1). cytotoxicity was d ... | 1996 | 8558524 |
| the product of a 1.9-kb mrna which overlaps the hsv-1 alkaline nuclease gene (ul12) cannot relieve the growth defects of a null mutant. | alkaline nuclease, a relatively abundant viral phosphoprotein in herpes simplex virus type 1 (hsv-1)- or hsv-2-infected cells, is encoded by a 2.3-kb mrna (r. h. costa, k. g. draper, l. banks, k. l. powell, g. cohen, r. eisenberg, and e. k. wagner, 1983. j. virol. 48, 591-603). this mrna is a member of a family of five unspliced 3'-coterminal messages. costa et al. proposed that another member of this family of mrnas (1.9-kb) may encode an n-terminally truncated protein which shares its carboxy- ... | 1996 | 8560762 |
| detection of herpes simplex virus after penetrating keratoplasty by polymerase chain reaction: correlation of clinical and laboratory findings. | the study was carried out to investigate the possible correlation of clinical findings, histopathologic features and detection of herpes simplex virus dna in corneal buttons obtained after penetrating keratoplasty. | 1995 | 8566829 |
| primary and secondary syphilis lesions contain mrna for th1 cytokines. | phagocytosis of treponema pallidum by cytokine-activated macrophages aids bacterial clearance and lesion resolution in early syphilis. to investigate the cytokine profiles of cells infiltrating primary and secondary syphilis lesions, reverse transcription and polymerase chain reaction (rt-pcr) were used to detect cytokine mrna in 13 lesion biopsies. both primary and secondary lesions contained mrna encoding interleukin (il)-2, interferon-gamma (ifn-gamma), il-12p40, and il-10. in contrast to a l ... | 1996 | 8568320 |
| detection and genotyping of herpes simplex virus types 1 and 2 by polymerase chain reaction. | a simple and rapid polymerase chain reaction (pcr) procedure was developed for simultaneous detection and typing of herpes simplex virus (hsv) types 1 and 2. it was possible to detect and type hsv using two primers pairs in a simultaneous double pcr reaction, where the type of hsv present was determined on the basis of an ethidium-bromide-stained band after agarose gel electrophoresis. this pcr assay was tested on about 500 clinical specimens. | 1995 | 8569766 |
| internal controls as performance monitors and quantitative standards in the detection by polymerase chain reaction of herpes simplex virus and cytomegalovirus in clinical specimens. | internal controls (ic) were produced and characterized for an hsv and a cmv pcr assay which serve both as test performance monitors and as quantitative standards. in each of the pcr assays the ic and native targets were amplified with equal efficiency and were detected with the same sensitivity, i.e. < 10 target copies. an algorithm was developed for the use of ic as a quantitative standard which entailed coamplifying a test specimen with four two-fold dilutions of the respective ic target (63-5 ... | 1995 | 8569776 |
| douching and sexually transmitted diseases in pregnant women in surabaya, indonesia. | our purpose was to investigate the association between douching (douching agents and timing) and sexually transmitted disease. | 1996 | 8571993 |
| characterization of the us8.5 protein of herpes simplex virus. | in a previous study a novel gene designated as us8.5 was identified in the unique short component of the herpes simplex virus type 1 (hsv1) genome. epitope tagging experiments suggested the existence of a protein encoded by this gene in hsv1 infected cells. to further analyze the us8.5 gene product and function, a rabbit polyclonal antiserum was raised against a recombinant beta-galactosidase-us8.5 fusion protein expressed in e. coli. this antiserum reacted specifically with a 19 kda protein in ... | 1995 | 8572943 |
| monoclonal antibody blocking tests for the detection of hsv-1- and hsv-2-specific humoral responses: comparison with western blot assay. | monoclonal antibody blocking radioimmunoassays (mab block rias) which detect specific humoral responses to each of the two human herpes simplex virus (hsv) types are described. rias were compared with western blot assay (wba) in a blind study of 64 sera obtained from clinically well-documented cases of genital herpes. wba and mab block ria each detected hsv-1 antibodies in 16/17 (94%) sera from confirmed hsv-1 genital infections (first episodes and recurrent infections). detection of hsv-2 antib ... | 1995 | 8576306 |
| synthesis and anti-herpes virus activity of 2'-deoxy-4'-thiopyrimidine nucleosides. | a series of 5-substituted 2'-deoxy-4'-thiopyrimidine nucleosides was synthesized and evaluated as potential antiviral agents. a number of analogues such as 2'-deoxy-5-propyl-4'-thiouridine (3ii), 2'-deoxy-5-isopropyl-4'-thiouridine (3iii), 5-cyclopropyl-2'-deoxy-4'-thiouridine (3iv), 2'-deoxy-4'-thio-5-vinyluridine (3viii), and 5-(2-chloroethyl)-2'-deoxy-4'-thiouridine (3xx) were found to be highly active against herpes simplex virus type-1 (hsv-1) and varicella zoster virus (vzv) in vitro with ... | 1996 | 8576922 |
| analysis of the relationship between cellular thymidine kinase activity and virulence of thymidine kinase-negative herpes simplex virus types 1 and 2. | the virulence of thymidine kinase-negative herpes simplex virus type 1 (hsv-1; vrtk- strain) and type 2 (hsv-2; uwtk- strain) was studied in comparison with that of their parental strains (vr-3 and uw-268, respectively) in an encephalitis model of adult (4-week-old) and newborn (3-day-old) mice. viral thymidine kinase (tk) activity was essential for the maximum expression of virulence of hsv-1, because the 50% lethal dose (ld50) of vrtk- was 60 times higher than that of vr-3 in the brains of new ... | 1995 | 8577269 |
| specific secretory immune responses in the female genital tract following intranasal immunization with a recombinant adenovirus expressing glycoprotein b of herpes simplex virus. | previously, we demonstrated that intranasal (i.n.) but not intraperitoneal (i.p.) immunization with a recombinant adenovirus vector expressing glycoprotein b (gb) of herpes simplex virus type 1 (hsv-1) induced mucosal immune responses and conveyed long-term protection to mice against an i.n. challenge with heterologous hsv-2. we now show that i.n. immunization of female mice with this same vector, adgb8, provides secretory and serum-derived humoral immune responses in the genital tract. intranas ... | 1995 | 8578847 |
| herpes simplex virus type 2 infections presenting as brainstem encephalitis and recurrent myelitis. | we describe here 3 patients with central nervous system infection caused by herpes simplex virus type 2 (hsv-2); one patient with brainstem encephalitis and two with recurrent transverse thoracic myelitis. all three patients showed increased igg antibodies to hsv in the cerebrospinal fluid (csf). hsv-2 dna was demonstrated in the csf by polymerase chain reaction (pcr) amplification. upon treatment with acyclovir, one patient with myelitis partially recovered and the others completely recovered. ... | 1995 | 8580553 |
| suspension culture of hela cells in protein-free medium: sensitivity to human pathogenic viruses. | human adherent hela-pf cells grown for 5 years in a protein-free 1:1 nutrient mixture of dulbecco's modified mem and ham's f12 (dmem-f12) were established in suspension culture. the cells grew in protein-free dmem-f12 (using magnetically stirred flasks) as a monodisperse suspension with a population doubling time of 28 h. the cells were infected with poliovirus types 2 and 3, herpes simplex virus types 1 and 2 (hsv-1 and hsv-2), respiratory syncytial virus (rsv), echovirus 6 and adenoviruses 3 a ... | 1994 | 8586536 |
| lichen planus after genital herpes simplex virus type 2 infection. | 1995 | 8589493 | |
| serologically diagnosed infection with human papillomavirus type 16 and risk for subsequent development of cervical carcinoma: nested case-control study. | to study human papillomavirus type 16 in the aetiology of cervical carcinoma. | 1996 | 8595281 |
| [characteristics related to std/hiv in men working in mexico city bars where female prostitution takes place]. | this research work intended to describe the characteristics and the seroprevalence of some sexually transmitted diseases (std), in men working in bars where female commercial sex takes place. | 1995 | 8600553 |
| the herpes simplex virus type 2 strain hg52 rl1 gene contains a 154 bp intron as predicted from sequence analysis. | the published sequence of the herpes simplex virus (hsv) type 2 strain hg52 neurovirulence gene rl1 indicated that, unlike the hsv-1 homologue, the gene contains a 154 bp intron. this intron contains six complete and one partial copy of a 19 bp repeat which encodes a stop codon; thus all three forward frames are blocked. by rt-pcr of poly(a)+ rna, using primers on either side of the proposed intron, we have isolated a partial cdna clone corresponding to the predicted spliced mrna. | 1996 | 8601785 |
| asymptomatic maternal shedding of herpes simplex virus at the onset of labor: relationship to preterm labor. | to determine if fetal growth restriction and prematurity are observed with subclinical shedding of herpes simplex virus (hsv) at the onset of labor. | 1996 | 8602295 |
| dna immunization against experimental genital herpes simplex virus infection. | a nucleic acid vaccine, expressing the gene encoding herpes simplex virus (hsv) type 2 glycoprotein d (gd2) under control of the cytomegalovirus immediate-early gene promoter, was used to immunize guinea pigs against genital hsv-2 infection. the vaccine elicited humoral immune responses comparable to those seen after hsv-2 infection. immunized animals exhibited protection from primary genital hsv-2 disease with little or no development of vesicular skin lesions and significantly reduced hsv-2 re ... | 1996 | 8603957 |
| automated microneutralization: method and comparison with western blot for type-specific detection of herpes simplex antibodies in two pregnant populations. | a novel method for the detection of microneutralization was compared to western blot for detection of type-specific antibodies to herpes simplex virus in two pregnant patient populations in vancouver. from an unselected group of women in labor in a tertiary care obstetric hospital (426) and another group of women specially referred for assessment of genital herpes in pregnancy (195) 20 and 88%, respectively, were seropositive for herpes simplex virus type 2 antibodies by western blot. during the ... | 1995 | 8609194 |
| the protein kinase activity of the large subunit of herpes simplex virus type 2 ribonucleotide reductase (icp10) fused to the extracellular domain of the epidermal growth factor receptor is ligand-inducible. | the gene coding for the large subunit of herpes simplex virus type 2 ribonucleotide reductase (rr) (icp10) has a unique 5' terminal domain the product of which has a serine/threonine (ser/thr) protein kinase (pk) catalytic domain preceded by a transmembrane (tm) segment. because icp10 localizes on the cell surface and is internalized by the endocytic pathway like an activated growth factor receptor (hunter et al., 1995, virology 210, 345-360), we asked whether it is ligand-inducible in order to ... | 1996 | 8610433 |
| interleukin-11: stimulation in vivo and in vitro by respiratory viruses and induction of airways hyperresponsiveness. | to address the role of il-11 in viral airways dysfunction, we determined whether infectious agents that exacerbate asthma stimulate stromal cell il-11 production, determined whether il-11 could be detected at sites of viral infection and evaluated the effects of il-11 on airway physiology. respiratory syncytial virus (rsv), parainfluenza virus type 3 (piv3), and rhinovirus (rv) 14 were potent stimulators while cytomegalovirus and adenovirus only weakly stimulated and herpes simplex virus type 2 ... | 1996 | 8613544 |