Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| relationships among ryanodine receptor isoforms expressed in vertebrate skeletal muscles based on immunologic cross-reactivities. | to clarify the relationship among ryanodine receptor isoforms from skeletal muscles of various vertebrates (alpha- and beta-isoforms with smaller and larger mobilities on sds-polyacrylamide gel electrophoresis, respectively), we examined the cross-reactivities of those receptors from 13 animal species by western blot analysis, using specific polyclonal antibodies raised in rabbit against the bullfrog alpha- and beta-isoforms. anti-beta-isoform antibody showed positive reactions against skeletal ... | 1994 | 7896740 |
| oral administration of insulin in winter-acclimatized carp (cyprinus carpio) induces hepatic ultrastructural changes. | 1. the intestinal absorption of insulin in carps was assessed examining the transepithelial passage of ingested gold-labeled hormone by electron microscopy. insulin transfer occurred mainly through the intercellular spaces between the enterocytes. 2. when reaching the lamina propria, the gold-labeled hormone gathered predominantly around the granules of the granular cells, and therefore can enter the circulatory system via the blood capillaries which are found in close contact with these cells. ... | 1993 | 7906626 |
| cystine/glutamate antiporter expression in retinal müller glial cells: implications for dl-alpha-aminoadipate toxicity. | a cytotoxicity of glutamate or related amino acids (10 mm) mediated by a cystine/glutamate antiporter (system xc) has recently been demonstrated in n18 neuroblastoma-rat retina hybrid (n18re105) cells and c6 glioma cells. the antiporter usually transports glutamate outside and cystine inside, thereby maintaining cellular concentrations of glutathione. high concentrations of glutamate inhibit cystine uptake and lead to depletion of cellular levels of glutathione. among related amino acids, dl-alp ... | 1993 | 7906874 |
| [relationship between the glutamine synthetase and glutamate dehydrogenase pathways of detoxification of exogenous ammonia in fish]. | regularities of adaptation reactions of detoxication of exogenic ammonium in fish have been established. under the pre-threshold level of the toxicant concentration level (0.05 mg/l) the function of the detoxication process is performed at the level of glutamine synthetase reaction mainly catalyzed by the enzyme cytoplasmatic form. the inhibition of both mitochondrial and cytoplasmic forms of the carp muscle glutamine synthetase by the mixed type was revealed under the effect of over-threshold c ... | 2015 | 7914038 |
| cloning of a growth hormone from a primitive bony fish and its phylogenetic relationships. | growth hormone (gh), prolactin, and their relatives constitute a multigene family which is considered to have evolved from a common ancestor. the structural and functional domains of gh appear to be highly conserved among vertebrates. in order to investigate the phylogenetic relationships among ghs in the actinopterygii and sarcopterygii, we have cloned and sequenced gh from the pituitary of the primitive bony fish, amia calva. bony fishes (teleosts) and amia (halecomorphi) are purported sister- ... | 1994 | 7926657 |
| spring viraemia of carp. | 1994 | 7941273 | |
| role of the atrial natriuretic peptide for adrenal regulation in the teleost fish cyprinus carpio. | the biological role of atrial natriuretic peptide (anp) for the adrenal tissue of the teleost fish cyprinus carpio, the carp, was investigated using in vitro autoradiography to study anp binding sites, immunohistochemistry to localize anp-like peptides, and in vitro perifusion to measure adrenal cortisol and catecholamine release. 125i-labeled rat anp-(99-126) [125i-ranp-(99-126)] binding was present in adrenal tissue exhibiting positive cooperativity with a half-maximal binding concentration (e ... | 1994 | 7943413 |
| alternate-day steroids affect carpal maturation more than radius, ulna and short bones. | radius, ulna, short bones (rus) and carpal (carp) bone age were assessed in 26 steroid-dependent nephrotic boys after at least 1 year of alternate-day prednisone therapy, and in 26 age- and sex-matched control subjects. no significant difference in rus bone age was found between patients and controls. carp bone age of patients was significantly (p = 0.01) more retarded than in controls. in patients, carp bone age delay was related to their relative height at the time of study (p = 0.01). we conc ... | 1994 | 7947042 |
| monoclonal antibodies against goldfish (carassius auratus) immunoglobulin: application to the quantification of immunoglobulin and antibody-secreting cells by elispot and seric immunoglobulin and antibody levels by elisa in carp (cyprinus carpio). | monoclonal antibodies (mabs) raised against heavy and light chains of goldfish immunoglobulin (ig) were characterized by a western blot technique. a complete cross-reactivity was observed between carp and goldfish ig. these mabs were used for the quantification of carp ig and anti-yersinia ruckeri antibodies by elisa. an elispot assay was also developed in carp to quantify ig-secreting cells (isc) and antibody-secreting cells (asc). the number of asc was maximum on day 18 post-vaccination and de ... | 1994 | 7951348 |
| prolactin gene expression and changes of prolactin pituitary level during the seasonal acclimatization of the carp. | the effect of seasonal acclimatization on the extent of prolactin (prl) gene expression and on the content of this was studied in summer- and winter-carp (cyprinus carpio) hormone pituitary glands. prl content in the rostral pars distalis (rpd) was evaluated by immunocytochemistry using antibodies against a cross-linked synthetic peptide comprising the sequence of 15 amino acids which conform to the primary structure of carp prl. to assess the level of prl gene transcription, a 24-mer synthetic ... | 1994 | 7953074 |
| immunodetection of the ribosomal transcription factor ubf at the nucleolus organizer regions of fish cells. | a human autoimmune serum to nucleolus organizer regions (nors) has been used to localize these structures at the light microscopic level in carp and trout tissue culture cells. in interphase cells, the immunofluorescence pattern indicates that the nors autoantigens are contained exclusively within the nucleolus of carp epithelial (epc) and trout gonad (rtg) cells. this fluorescence is punctuate rather than uniform, and presumably represents transcriptional complexes of ribosomal dna. during mito ... | 1994 | 7954874 |
| giant catfish pangasianodon gigas growth hormone-encoding cdna: cloning and sequencing by one-sided polymerase chain reaction. | cdna clones encoding giant catfish (pangasianodon gigas) growth hormone (gh) have been isolated using a polymerase chain reaction (pcr) strategy. pairwise combinations of degenerate and general primers allowed for the amplification of regions both 3' and 5' to the point of entry into the message. the amplified pcr products were cloned and sequenced. the cdna sequence was found to encode a polypeptide of 200 amino acids (aa), including a putative signal peptide of 22 aa. the 5' and 3' untranslate ... | 1994 | 7959001 |
| similarities and differences between fish oligodendrocytes and schwann cells in vitro. | in light of the striking differences between oligodendrocytes of the optic nerve/tract of adult goldfish and their mammalian counterparts, a further characterization of goldfish oligodendrocytes was performed. a comparison with schwann cells was included because fish optic nerve/tract-derived oligodendrocytes bear remarkable similarities to this type of glial cell. fish optic nerve/tract-derived oligodendrocytes that had differentiated into 04 and 6d2-positive cells and thus expressed early myel ... | 1994 | 7960034 |
| immunocytochemical analysis of mitogen responses of carp (cyprinus carpio l.) peripheral blood leucocytes. | phytohaemagglutinin (pha) and lipopolysaccharide (lps) responses of surface immunoglobulin-positive (sig+) and surface immunoglobulin-negative (sig-) carp peripheral blood leucocytes (pbl) were studied. sig+ cell-enriched and depleted carp pbl populations (sig+ and sig- cell fractions, respectively) were obtained by magnetic cell sorting (macs) and mitogenic stimulation in vitro was measured by 3h-thymidine incorporation. the mitogen responses of sig+ and sig- cells in non-separated carp pbl cul ... | 1994 | 7975192 |
| does a natural dnase-actin complex exist in the carp liver cytosol? | 1994 | 7976016 | |
| muscle glucose utilization during sustained swimming in the carp (cyprinus carpio). | the involvement of circulatory glucose in the energy provision of skeletal muscle and heart of swimming carp was examined. plasma glucose concentration varied from 3 to 17 mm among individual carp, and estimates of glucose turnover rate (rt) were positively correlated with plasma glucose level in resting fish (range 1.6-6.3 mumol.min-1.kg-1) and in swimming fish (range 4.2-10.7 mumol.min-1.kg-1). carp that were exercised at 80% of their critical swimming speed displayed a twofold higher rt at an ... | 1994 | 7977849 |
| ultrastructure of nonspecific cytotoxic cells in teleosts. i. effector-target cell binding in a marine and a freshwater species (seabream: sparus aurata l., and carp: cyprinus carpio l.). | fish cytotoxic effectors form a cell population whose ultrastructure and properties of conjugation with target cells have not been completely established. we report the ultrastructure of the nonspecific cytotoxic cells in a seawater teleost (sparus aurata l.) and compare it to a freshwater species (cyprinus carpio l.). | 1994 | 7978369 |
| divergent selection for antibody production in common carp (cyprinus carpio l.) using gynogenesis. | a base population (n = 101) of carp, consisting of a single hybrid cross, was immunized with the hapten-carrier complex dnp-klh, to perform a divergent selection for antibody response. measurement of the dnp-specific antibody response at 12 and 21 days postimmunization, allowed the classification of a low number of individual carp as early/high (10%) or late/low (13%) responders. three individuals defined as early/high and three defined as late/low responding, were gynogenetically reproduced to ... | 1994 | 7985841 |
| dnase-i-like enzyme from the carp liver--inhibition by muscle and endogenous actin. | 1. dnase-i-like activity occurs in the carp (cyprinus carpio) liver cytosol (supernatant 105,000 g). 2. the enzyme resembles dnase i from bovine pancreas in respect to the molecular mass (approximately 31 kda), ph (7.4) and ion requirements (mg2+, ca2+) and the ability to degrade native as well as denatured dna. 3. as judged by comparison of dnase zymograms obtained after native- and sds-page, the enzyme occurs in the three molecular forms of similar molecular weight and different charges. 4. al ... | 1994 | 7988739 |
| differential effects of temperature on macrophages of ectothermic vertebrates. | the role of macrophages in vertebrate defense reactions is especially important at low temperatures, which commonly affect representatives of ectothermic species. the aim of this work was to compare the effects of in vivo thermal acclimation and in vitro assay temperatures on peritoneal macrophages (pms) from fish (carp and goldfish) and amphibians (salamanders and toads). the substratum adherence of pms was undisturbed over the wide range of temperatures experienced by animals in nature. dehydr ... | 1994 | 7996049 |
| parasexual crosses and hybrid selection with a near haploid variant of the epithelioma papulosum cyprini, cell line epc: a tissue culture model for the analyses of dominance, recessiveness, and complementation of mutant phenotypes. | we describe techniques for insertional mutagenesis of tissue-cultured piscine cells in which we use transfection with g418 and hygromycin b resistance-conferring plasmids, cell matings by electrofusion, and positive selections of fusion hybrids by dual challenge with the antibiotics g418 and hygromycin b. these techniques are designed to facilitate genetic and molecular analyses of tissue-cultured cells. the experiments were conducted with epc-1, a new variant of the carp epithelioma cyprini cel ... | 1994 | 8000480 |
| cloning and characterization of the european seabass, dicentrarchus labrax, mitochondrial genome. | mitochondrial dna (mtdna) from the european seabass, dicentrarchus labrax, has been cloned and characterized. its gene organization was deduced by a comparison of the sequenced termini of different subclones obtained from european seabass mtdna to the completely-sequenced mtdnas from carp and freshwater loach. the difference in genome size between the european seabass mtdna (approximately 18 kb) and most of the other characterized fish mtdnas (approximately 16.5 kb) is accounted for by the displ ... | 1994 | 8001168 |
| properties of the 'neutral zone' explain polarity of retinal spreading depression. | retinal spreading depression was evoked using low cl- ringer's solution and the concomitant field potentials (spreading depression potential; sdp) were recorded. the polarity of the transretinally recorded sdps was not consistent among animal species. the sdps recorded from carp and frog were receptor side negative, while chick and cat induced receptor side positive sdps. according to the k+ hypothesis, the retinal sdp is generated by müller cells responding to an increase in the extracellular k ... | 1994 | 8008248 |
| subunit structures of multiple hemoglobins in carp. | 1994 | 8014260 | |
| studies on the toxic and immunosuppressive effects of cadmium on the common carp. | in the last couple of decades, research on cadmium extended from its toxicologic aspects to its role in environmental pollution. the toxicity of cadmium to different species of fish varies widely. the cadmium sensitivity of batches of common carp, the most important cultured fish species of hungary, was studied. lethality, histopathologic changes and effect of cadmium on the antibody response, lysozyme level and microbicidal capacity were monitored. wide variation was found in individual sensiti ... | 1993 | 8017244 |
| immunocytochemical study on biologically active neurosubstances in daughter sporocysts and cercariae of trichobilharzia ocellata and schistosoma mansoni. | immunocytochemical techniques applied to sections and whole-mount preparations of cercariae from two species of trematodes, trichobilharzia ocellata and schistosoma mansoni, revealed the occurrence of immunoreactivity (ir) to several neurosubstances in the nervous system (ns). immunostaining was localized in cerebral ganglia, in the main commissure, in anterior and posterior nerve trunks, as well as in a pair of nerve fibres running along the tail. in t. ocellata, immunoreactivity (ir) was obser ... | 1994 | 8022656 |
| [structure analysis of the mitochondrial trna(cys) gene and the original region of light-strand replication from carp]. | in this study, we have determined the nucleotide sequences of the cysteine trna gene and the original region of light-strand replication from carp (cyprinus carpio l.) mitochondrion, which were further represented respectively in cloverleaf secondary structure and stem loop structure. by comparing the nucleotide sequences of 5 vertebrate trna(cys) genes, it is found that carp mitochondrial trna(cys) gene have many unusual structural features differing from those of cytoplasmic trna(cys) gene. th ... | 1993 | 8023638 |
| insulin binding and receptor tyrosine kinase activity in skeletal muscle of carnivorous and omnivorous fish. | we characterized the insulin receptors in skeletal muscle from several fish species with different nutritional preferences: brown trout (salmo trutta fario), gilthead sea bream (sparus aurata), tilapia (tilapia mossambica), and carp (cyprinus carpio), semipurified by affinity chromatography (wheat germ agglutinin-agarose). total specific binding and number of receptors per unit weight of piscine white skeletal muscle were lower than those values found in mammalian skeletal muscle. the same param ... | 1994 | 8024051 |
| effect of mercuric chloride and cadmium chloride on gonadal function and its regulation in sexually mature common carp cyprinus carpio. | gonadal function in fish, cyprinus carpio was significantly affected by sublethal doses of mercuric chloride (hgcl2) and cadmium chloride (cdcl2) in chronic (45 days) exposure. parameters investigated were nonesterified (ne) and esterified (e) cholesterol of ovary, liver and serum and ovarian 3 beta-hydroxysteroid and 17 beta-hydroxysteroid dehydrogenase enzyme activity and serum and pituitary gonadotropin (gth) levels. both the pollutants were able to reduce the hypothalamic extract (he) or gon ... | 1994 | 8024715 |
| characterization of gamma-crystallins from a hybrid teleostean fish: multiplicity of isoforms as revealed by cdna sequence analysis. | gamma-crystallins were isolated and characterized from the eye lenses of a hybrid species belonging to the teleostean fish. isoelectric focusing of gamma-crystallin fraction obtained from gel-permeation chromatography revealed that it consists of multiple charge isomers of a protein species with a molecular mass of about 20 kda. to facilitate the cloning of gamma-crystallin gene, cdna was constructed from the poly(a)+mrna isolated from fresh lenses, and amplification by polymerase chain reaction ... | 1994 | 8037758 |
| toxicity identification evaluations for the investigation of fish kills: a case study. | a large fish-kill was observed in the river tajo during the spring-summer of 1991. the mortality was first detected between aranjuez and toledo, affecting several fish species. then it was slowly going downstream, affecting only carp (cyprinus carpio), reaching the spanish-portuguese border several months later. short-term toxicity tests on daphnia magna and in vitro cytotoxicity tests on rtg-2 cells were used as toxicity monitoring systems in water samples and different water fractions. the fis ... | 1994 | 8044634 |
| phylogenetic and ontogenetic study of neuropeptide y-containing nerves in the liver. | the distribution of neuropeptide y was investigated by light and electron microscopic immunohistochemistry in the liver of various vertebrates including the eel, carp, bullfrog, turtle, chicken, mouse, rat, guinea-pig, dog, monkey and human. the ontogenetic development of neuropeptide y was also studied in the mouse liver. in all species examined except the eel, neuropeptide y-like immunoreactivity was detected in nerve fibres. in the carp, bullfrog, turtle, chicken, mouse and rat, positive fibr ... | 1994 | 8045785 |
| [activity of lysosomal proteinases in carb tissue in aeromonas infection]. | the activity of major lysosomal proteinases (cathepsins b and d) increased in carp tissues infested with aeromonas hydrophila. the value and character of changes in the activity of cathepsins depended on the degree of infestation and the physiological state of fishes (normally fed or starved). | 1994 | 8047538 |
| characterisation of immunoglobulin-binding leucocytes in carp (cyprinus carpio l.). | this study demonstrates the immunoglobulin(ig)-binding capacity of ig-positive carp macrophages employing immunofluorescence and immunogold methods. these methods allow for the characterisation of the ig-binding cells. after internalisation of fluorescent- or gold-labelled ig (30 min at room temperature), most macrophages from the hindgut were able to bind added purified carp ig, which could be demonstrated clearly with a second fluorescent or gold label. in pronephros, an important haemopoietic ... | 1994 | 8050616 |
| creatine kinase, energy-rich phosphates and energy metabolism in heart muscle of different vertebrates. | maximal activities of creatine kinase, pyruvate kinase and cytochrome oxidase and total concentrations of creatine and phosphorylated adenylates were measured in cardiac muscle of hagfish, eight teleost species, frog, turtle, pigeon and rat. the ratio of creatine kinase to cytochrome oxidase with cytochrome oxidase as a rough estimate of aerobic capacity and cellular "energy turnover", was increased in myocardia of hagfish, turtle and crucian carp. these myocardia are likely to be frequently exp ... | 1994 | 8056878 |
| structural order of membranes and composition of phospholipids in fish brain cells during thermal acclimatization. | a comparison of the structural orders of membranes of a mixed brain-cell population isolated from cyprinus carpio l. acclimated to either summer (23-25 degrees c) or winter (5 degrees c) revealed a high degree of compensation (80%) for temperature, as assayed by electron spin resonance spectroscopy. the cells rapidly forget their thermal history and adjust the physical properties of the membranes when shifted to the other extreme of temperature either in vivo or in vitro. phospholipids separated ... | 1994 | 8058786 |
| contaminants in fishes from great lakes-influenced sections and above dams of three michigan rivers. i: concentrations of organo chlorine insecticides, polychlorinated biphenyls, dioxin equivalents, and mercury. | fishes of the great lakes contain hazardous chemicals such as synthetic halogenated hydrocarbons and metals. these fish can move from the lakes into the great lakes tributaries of michigan. in doing so, they transport concentrations of contaminants which may represent a risk to wildlife. concentrations of mercury (hg), total polychlorinated biphenyls (pcbs), 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (tcdd-eq), total ddt complex, aldrin, endrin, dieldrin, heptachlor, heptachlor epoxide, lin ... | 1994 | 8060164 |
| contaminants in fishes from great lakes-influenced sections and above dams of three michigan rivers. ii: implications for health of mink. | populations of mink (mustela vison) have declined in many areas of the world. such declines have been linked to exposures to synthetic, halogenated hydrocarbons. in the great lakes region, mink are fewer in areas along the shore of the great lakes and their tributaries where mink have access to fish from the great lakes. recently, there has been discussion of the relative merits of passage of fishes around hydroelectric dams on rivers in michigan. a hazard assessment was conducted to determine t ... | 1994 | 8060165 |
| anoxia and adenosine induce increased cerebral blood flow rate in crucian carp. | the crucian carp (carassius carassius) has the rare ability to survive prolonged anoxia, indicating an extraordinary capacity for glycolytic atp production, especially in a highly energy-consuming organ like the brain. for the brain to be able to increase its glycolytic flux during anoxia and profit from the large liver glycogen store, an increased glucose delivery from the blood would be expected. nevertheless, the effect of anoxia on brain blood flow in crucian carp has never been studied prev ... | 1994 | 8067472 |
| subunit structure of multiple hemoglobins in carp. | three hemoglobin components in carp designated ci, cii, and ciii, were isolated by deae-tokyo-pearl ion-exchange chromatography. constituent globin chains, alpha 1, alpha 2, beta 1, and beta 2, were analyzed by urea-triton acid polyacrylamide gel electrophoresis and isolated by high performance liquid chromatography with a reverse-phase column. tryptic peptide mapping indicated that the alpha-globin chains of the three hemoglobin components have slightly different structures. in addition, n-term ... | 1993 | 8071464 |
| mimosine-induced cell death and related chromatin changes. | the non-protein amino acid mimosine has recently been shown to induce an unusual mode of cell death that differs from necrosis and apoptosis, the two fundamental schemes of cell death. the drug affects primarily the cell nucleus and induces first condensation of the chromatin into a regular network of fibrils and then gradual decondensation. the present study was performed to evaluate effective mimosine concentrations, time dependency of toxicity, and mimosine-related alterations of the chromati ... | 1994 | 8087796 |
| growth hormone and gonadotropin secretion in the common carp (cyprinus carpio l.): in vitro interactions of gonadotropin-releasing hormone, somatostatin, and the dopamine agonist apomorphine. | the effects of salmon gonadotropin-releasing hormone (sgnrh) and the superactive agonist [d-arg6, pro9net]-sgnrh (sgnrh-a) on growth hormone (gh) and gonadotropin (gth) release were examined using a perifusion system for pituitary fragments of the common carp (cyprinus carpio). perifusion of 2-min pulses of different concentrations of sgnrh or sgnrh-a stimulated a rapid and dose-dependent increase in gh release: ed50 values for sgnrh and sgnrh-a in stimulating gh release were 2.8 +/- 0.7 and 0.5 ... | 1993 | 8094060 |
| immunoreactive atrial natriuretic peptides in the oocyte. | 1. the presence and partial characterization of immunoreactive anp (iranp) in eggs were investigated using high performance liquid chromatography (hplc), immunohistochemistry and northern-blot hybridization in mammalian and nonmammalian vertebrates. 2. serial dilution curves of egg extracts from frogs and freshwater teleostean fishes (silver crusian carp and loach) were parallel to the standard curve of atriopeptin iii. rat oocytes also contained iranp (25-30 pg/egg). 3. the hplc profile of iran ... | 1993 | 8095876 |
| sensitivities of achatina giant neurones to peptides isolated from mollusca. | sensitivities of the achatina giant neurones to the peptides isolated from mollusca were examined by their local application of the pneumatic pressure ejection under current clamp. the peptides tested and their effects were as follows: ser2-mip (inhibitory). carp (inhibitory), oxytocin (excitatory), spcb (excitatory), alpha-bcp (no effect). elh (no effect). apgw-amide (inhibitory) and fmrfamide (inhibitory). membrane conductance (g) of a neurone, d-rpean, measured under voltage clamp, was unexpe ... | 1993 | 8098562 |
| polymorphism and estimation of the number of mhccyca class i and class ii genes in laboratory strains of the common carp (cyprinus carpio l.). | restriction fragment length polymorphisms (rflps) have been identified in the mhc of the carp (mhccyca) using class i (cyca-z) and class ii (cyca-yb) specific probes. the k1-5 and k2-1 probes were obtained as pcr products after amplification of genomic dna from a european carp using primers deduced from genomic sequences, and were shown to be 90% and 80% similar to cyca-z exon 3 and cyca-yb exon 2 sequences, respectively. six carp strains of different geographical origins and genomic status were ... | 2003 | 8099021 |
| recent achievements in the chemotherapy of myxosporean infections of fish. | until quite recently, the therapy of fish diseases caused by myxosporeans has been an unsolved problem. fumagillin is the only effective drug which can be successfully applied against several myxosporeoses in the form of prolonged feeding. it has been used with good results against sphaerosporosis of common carp, hoferellosis of goldfish, myxidiosis of eel, and proliferative kidney disease, whirling disease and ceratomyxosis of salmonids. already a 4- to 6-day feeding of medicated diets containi ... | 1993 | 8116501 |
| dendrites of rod dominant on-bipolar cells are coupled by gap junctions in carp retina. | gap junctions are supposed to be the anatomical substrate for electrical coupling between neurons. in fish retina, bipolar cells are electrically coupled and their receptive field diameters are always larger than dendritic field size, however, gap junctions have not been described between dendrites of bipolar cells. in this paper, using immunostaining for protein kinase c, we show that every rod dominant on-bipolar cell is connected with its neighboring dendrites by gap junctions forming a plexu ... | 1993 | 8121632 |
| carp parvalbumin binds to and directly interacts with the sarcoplasmic reticulum for ca2+ translocation. | interaction between two relaxing factors, the sarcoplasmic reticulum and parvalbumin, in carp fast skeletal muscle was investigated. immunoblotting using an anti-parvalbumin antibody revealed that parvalbumin bound to the light sarcoplasmic reticulum isolated from carp fast skeletal muscle in the presence of ca2+. parvalbumin enhanced ca2+ uptake activity of the light sarcoplasmic reticulum. furthermore, using a photoreactive cross-linker, we detected a protein in the light sarcoplasmic reticulu ... | 1994 | 8123046 |
| distribution of free d-serine in vertebrate brains. | free d-serine distribution in vertebrate brains was investigated. in various brain regions of the lower vertebrate species, carp, frog and chick, free d-serine levels were low. on the contrary, in the mammals, mouse, rat and bull, the contents of free d-serine were high in the forebrain (around 400 nmol/g wet weight, and the ratio of d-serine to l-serine, was d/l = 0.4), and low in the hindbrain. in developing mice, d-serine levels in the cerebrum increased with age and attained the adult level ... | 1994 | 8131079 |
| purification and characterization of urotensin ii from the brain of a teleost (trout, oncorhynchus mykiss) and an elasmobranch (skate, raja rhina). | peptides related to urotensin ii have been isolated in pure form from an extract of whole brain of a teleost, the rainbow trout (oncorhynchus mykiss) and of an elasmobranch, the longnose skate (raja rhina). the primary structure of the trout peptide [gly-gly-asn-ser-glu-cys-phe-trp-lys-tyr-cys-val] is similar to that of urotensin ii peptides isolated from the urophyses of other teleost fish. for example, trout urotensin ii contains only one amino acid substitution (thr4-->ser) compared with urot ... | 1993 | 8138107 |
| isolation of a carp complement protein homologous to mammalian factor d. | factor d of carp (cyprinus carpio) complement was purified to apparent homogeneity by a 4-step chromatographic procedure and examined for physicochemical and functional properties. carp factor d proved to be an alpha-globulin with a molecular mass of 29 kda and the serum concentration was estimated to be 6 micrograms/ml. the nh2-terminal amino acid sequence (30 residues including five unidentified positions) of carp factor d showed high homologies (57-60%) to those of mammalian factor d. neither ... | 1994 | 8152436 |
| [the protein level in bothriocephalus acheilognathi cestodes and their hosts, carp fingerlings, in experimental feeding and starving of the fish]. | 1993 | 8152846 | |
| localization of extratectally evoked visual response in the corpus and valvula cerebelli in carp, and cerebellar contribution to 'dorsal light reaction' behavior. | in carp with the optic tectum removed bilaterally, the optic nerve stimulation evoked a surface positive response on the cerebellum, which originated in the granular layer. in the corpus cerebelli, the response was composed of a single component, well localized at the rostromedial part, supporting previous electrophysiological and morphological findings. in the valvula cerebelli, on the other hand, the response consisted of two wave components of oligo-synaptic and poly-synaptic transmission. th ... | 1993 | 8155291 |
| effects of water-borne cadmium on the skin of the common carp (cyprinus carpio). | the skin of carp, cyprinus carpio, was studied at the ultrastructural level after exposure of the fish to low and high concentrations of cadmium in the water (22 and 560 micrograms/l, respectively) for different periods. the effects of the low concentration of cadmium were similar to those of the high concentration, although they appeared later. the basal lamina and the skin surface became highly undulating. chloride cells appeared between the pavement cells. necrotic pavement cells were seen fr ... | 1994 | 8161232 |
| cloning and sequencing the genes encoding goldfish and carp ependymin. | ependymins (epns) are brain glycoproteins thought to function in optic nerve regeneration and long-term memory consolidation. to date, epn genes have been characterized in two orders of teleost fish. in this study, polymerase chain reactions (pcr) were used to amplify the complete 1.6-kb epn genes, gf-i and cc-i, from genomic dna of cypriniformes, goldfish and carp, respectively. amplified bands were cloned and sequenced. each gene consists of six exons and five introns. the exon portion of gf-i ... | 1994 | 8163195 |
| in vivo inhibition of ache and atpase activities in the tissues of freshwater fish, cyprinus carpio exposed to technical grade cypermethrin. | 1994 | 8167458 | |
| the complete nucleotide sequence and gene organization of carp (cyprinus carpio) mitochondrial genome. | the complete sequence of the carp mitochondrial genome of 16,575 base pairs has been determined. the carp mitochondrial genome encodes the same set of genes (13 proteins, 2 rrnas, and 22 trnas) as do other vertebrate mitochondrial dnas. comparison of this teleostean mitochondrial genome with those of other vertebrates reveals a similar gene order and compact genomic organization. the codon usage of proteins of carp mitochondrial genome is similar to that of other vertebrates. the phylogenetic re ... | 1994 | 8169959 |
| seasonal variations in gonadotropin responsiveness, self-priming, and desensitization to gnrh peptides in the common carp pituitary in vitro. | seasonal variations of the gth release response to salmon gnrh (sgnrh) and [d-arg6,pro9net]-sgnrh (sgnrh-a) were investigated in female common carp at different stages of the reproductive cycle using perifused pituitary fragments. the responsiveness to sgnrh and sgnrh-a varied seasonally in common carp pituitaries in vitro, with the greatest gth release response in pituitaries from sexually mature (preovulatory) fish compared to pituitaries from sexually regressed fish. the magnitude of this sea ... | 1994 | 8174932 |
| isolation and stability of partially oxidized intermediates of carp hemoglobin: kinetics of co binding to the mono- and triferric species. | the monoliganded and triliganded forms of the asymmetric valency hybrids of carp hemoglobin were isolated using high-performance liquid chromatography. these partially oxidized hybrids were shown to be sufficiently stable to permit the measurement of the kinetics of co binding. the effects of protons and inositol hexaphosphate on the rates of these reactions were examined. the kinetics of co recombination with these partially oxidized derivatives were compared to the kinetics of co binding to th ... | 1994 | 8180217 |
| a case-control study of oral cancer in beijing, people's republic of china. associations with nutrient intakes, foods and food groups. | a case-control study of oral cancer was conducted in beijing, people's republic of china to examine the association between dietary nutrient intake and risk of oral cancer, both in terms of estimated intake of nutrients and micro-nutrients, and in terms of specific foods and food groups. the study was hospital-based and controls were hospital in-patients matched for age and sex with the cases. the response rate for cases and controls was 100% and 404 case/control pairs were interviewed. the resu ... | 1993 | 8180577 |
| response of serum calcium to administration of 1,25-dihydroxyvitamin d3 in the freshwater carp cyprinus carpio maintained either in artificial freshwater, calcium-rich freshwater or calcium-deficient freshwater. | freshwater carps (cyprinus carpio) were injected daily intraperitoneally either with vehicle (0.1 ml 95% ethanol/100 gm body wt), or 1,25(oh)2d3 (5 iu/100 gm body wt) and maintained either in artificial-freshwater, calcium-rich freshwater or calcium-deficient freshwater for 15 days. the specimens were sacrificed on day 1, 3, 5, 10 and 15 after initiation of the experiment and serum calcium levels were analysed. (i) artificial freshwater: serum calcium level of 1,25(oh)2d3 treated specimens incre ... | 1993 | 8197882 |
| [cystic changes of the skin caused by dermocystidium koi in koi carp]. | skin infection in koi carp by dermocystidium koi is described. symptoms of disease and the parasite are illustrated. | 1994 | 8209371 |
| comparative studies on fish and bird insulin receptors. | the comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). the scatchard plots indicated the presence of two pools of binding sites both in fish and duck. these pools show inter-species differences in binding ability and the number of receptors. specific binding of insulin and the binding affinity are higher in duck than in fish. | 1993 | 8212960 |
| the effect of 75% glycerol on the oxygen binding properties of carp hemoglobin. | at ph 6 in the presence of inositol hexaphosphate, ihp, conditions where ligand-saturated carp hemoglobin is already in the low affinity t state, the addition of glycerol has little effect on affinity and ligand binding remains noncooperative. at all other ph values examined, with and without ihp, the effect of glycerol is to lower oxygen affinity possibly by shifting the equilibrium between the t state and the high affinity r state in the direction of the t state. although glycerol does not app ... | 1993 | 8216252 |
| studies on the hypocalcemic actions of salmon calcitonin and ultimobranchial gland extracts in the freshwater teleost cyprinus carpio. | a single injection of synthetic salmon calcitonin (sct) or homologous ultimobranchial gland (ubg) extract caused a significant reduction in total and ultrafiltrable plasma ca2+ in a freshwater teleost, cyprinus carpio. a time-bound analysis on the effect of sct or ubg extract caused a short-duration hypocalcemia and the response was more pronounced in fish kept in low-ca2+ water. sexually immature fish showed a greater response than did mature fish. in all the studies the protein-bound ca2+ frac ... | 1993 | 8224753 |
| biomonitoring of aquatic systems. | the 32p-postlabelling analysis provides a sensitive means for detecting pollution-related dna adducts in aquatic organisms exposed to environmental carcinogens. however, the following factors need to be taken into consideration during the data interpretation: (1) species-specific, naturally occurring dna modifications (or i-compounds) are found in aquatic organisms at levels which are highly season-dependent; and (2) many aquatic organisms, particularly lower invertebrates, cannot form dna adduc ... | 1993 | 8225508 |
| dna adduct detection: some applications in monitoring exposure to environmental genotoxic chemicals. | in the assessment of genotoxic risk factors in the environment, the measurement of dna adducts in aquatic organisms and in plants may have considerable implications. using 32p-postlabelling, we have detected dna adducts in the liver of carp (chondrostoma nasus) from the river rhône (france), both downstream and upstream from a polychlorinated biphenyl incineration plant. some of the dna adducts were specific to downstream fish, suggesting a differential pattern of exposure. we have also detected ... | 1993 | 8225509 |
| estimation of the genetic variation in complement activity of common carp (cyprinus carpio l.). | the complement status of hybrid, laboratory raised carp was determined by an in vitro approach of the alternate complement activity (ach50) and total haemolytic activity (ch50), and by measurement of serum c3 levels. the lysis of target sheep red blood cells (rbc) in the haemolytic assay for ch50 activity depended, amongst others, on the haemolysin concentration in the assay. rocket electrophoresis showed a mean serum c3 concentration of 0.95 mg ml-1. the variation for both ach50 and ch50 haemol ... | 1993 | 8236805 |
| [localization and activity of several enzymes in tissues of bothriocephalus acheilognathi and khawia sinensis tapeworms parasitizing carp]. | in this study, an attempt has been made to compare the most common species of tapeworms in carp: non-segmentary khawia sinensis and segmentary bothriocephalus acheilognathi on the basis of histoenzymatic examination of 8 enzymes, i.e. alkaline phosphatase, acid phosphatase, adenosine triphosphatase, nonspecific esterase alfa, succinic dehydrogenase, lactate dehydrogenase, leucine aminopeptidase and gamma-glutamylo transferase. | 1993 | 8237009 |
| multiphasic allometry. | a multiphasic linear model is presented that describes the allometric relation between body components and permits a smooth transition from one linear segment to the next. three applications of a diphasic linear model to allometric growth problems were used to illustrate the model. the data sets were selected because a breakpoint exists in the allometric relation. relations between growth of the alimentary tract and of empty body weight in rabbits, growth of length and of body weight in larval-c ... | 1993 | 8244622 |
| neuropeptide y in the carp torus semicircularis: an immunocytochemical study. | the presence of neuropeptide y-like immunoreactivity in the torus semicircularis of the teleost fish cyprinus carpio was studied using an indirect immunoperoxidase technique. in general, both the nuclei lateralis and centralis of the torus semicircularis showed a high density of immunoreactive fibers from rostral to caudal levels. in particular, in the nucleus lateralis a low density of immunoreactive fibers was only found in the subependymal layer, whereas this was high in the small cell, fibri ... | 1993 | 8250673 |
| the expression of rat gap-43 cdna in transgenic carp. | to understand more about growth-associated protein-43 (gap-43), we produced transgenic carp by introduction of a rat gap-43 cdna linked to the rous sarcoma virus-long terminal repeat into fertilized eggs. of 180 eggs microinjected with exogenous gene, 59 embryos hatched and 4 fish were found to contain the exogenous gene sequences in the genomic dna. from a mature female transgenic carp, parthenogenetically, 126 progeny were derived and 52 of them survived for more than 90 days. the exogenous ge ... | 1993 | 8257558 |
| effect of lethal and sublethal concentrations of cadmium on energetics in the gills of fry and fingerlings of cyprinus carpio. | 1993 | 8257822 | |
| in vitro phosphorylation in isolated horizontal cells of the fish retina: effects of the state of light adaptation. | horizontal cells, which are second-order neurons of the vertebrate retina, exhibit synaptic plasticity governed by light and dark adaptation. we have investigated the alterations in the protein phosphorylation patterns of isolated carp (cyprinus carpio) horizontal cells in relation to their state of light adaptation by using an in vitro phosphorylation assay and compared the resulting data with protein synthesis patterns of the whole retina. phosphoproteins and [35s]methionine-labelled proteins ... | 1993 | 8261133 |
| cytoarchitecture, fiber connections, and ultrastructure of the nucleus pretectalis superficialis pars magnocellularis (psm) in carp. | the cytoarchitecture, fiber connections, and ultrastructure of the nucleus pretectalis superficialis pars magnocellularis (psm) were studied in cypriniform teleosts (cyprinus carpio). the psm is an oval nucleus in the pretectum. medium-sized cells and synaptic glomeruli are the main components of the nucleus. a lesser number of small cells are also present. most of the medium-sized cells form one or two cell layers on the periphery of the nucleus, and some cells are scattered among synaptic glom ... | 1993 | 8263230 |
| the branching of insulin-like growth factor 1 and insulin: an immunohistochemical analysis during phylogeny. | the co-existence of insulin-like growth factor 1 (igf-1) with the classical islet hormones insulin (ins), glucagon (gluc), somatostatin (som) and pancreatic polypeptide (pp) in the endocrine pancreas of representative species of cyclostomes (myxine glutinosa), cartilaginous fish (raja clavata, squalus acanthias) and bony fish (cottus scorpius, carassius auratus, cyprinus carpio, anguilla anguilla) was studied by the use of monoclonal and polyclonal antisera and the double immunofluorescence tech ... | 1993 | 8265818 |
| influence of seasonal adaptation on the distribution of cytochemically detectable endogenous calcium in synaptic terminals of the optic tectum of crucian carp (carassius carassius l.). | crucian carps were seasonally adapted to high (23 degrees c) or low (4 degrees c) temperatures and synaptic regions in the optic tectum were analyzed by means of energy-filtering transmission electron microscopy (eftem). the amount of cytochemically detectable endogenous calcium in synaptic clefts was quantified by means of image analysis and the amount of glycogen granules in synaptic regions was assessed. in cold-adapted fish, glycogen is increased, especially in glial cells, while the calcium ... | 1993 | 8270788 |
| antibody dependent haemolysin, complement and opsonin in sera of a major carp, cirrhina mrigala and catfish, clarias batrachus and heteropneustes fossilis. | the present communication is a continuation of our earlier study on the natural serum haemagglutinin/lectins of cirrhina mrigala, clarias batrachus and heteropneustes fossilis. sera of cirrhina mrigala, belonging to the major carp family, could not only agglutinate heterologous rabbit erythrocytes, but also lyse them spontaneously. this lysis of rabbit rbc by cirrhina mrigala sera was calcium ion dependent and heat sensitive, indicating thereby that the haemolysis was mediated by the fish serum ... | 1993 | 8281746 |
| effects of prolonged darkness on light responsiveness and spectral sensitivity of cone horizontal cells in carp retina in vivo. | light responses of cone- and rod-driven horizontal cells were recorded intracellularly from opened eyes of intact, immobilized carp and the effects of prolonged darkness on these cells were examined. when the retina was left in the dark, responses of cone horizontal cells to a moderate test flash presented once every 10 min gradually and steadily decreased in size (dark suppression effect). following a repetitive presentation of the test flash, the light responsiveness of the cells was enhanced. ... | 1994 | 8283240 |
| localized leucocyte response to ichthyophthirius multifiliis establishment in immune carp cyprinus carpio l. | o-group carp (cyprinus carpio) which had been immunized against ichthyophthirius multifiliis by controlled infections were challenged by topical application of theronts to the caudal fin. the parasites which established were examined ultrastructurally, and host leucocyte responses were compared with those observed in primary infections. in the primary exposure group eosinophils and (to a lesser extent) basophils were the predominant cells infiltrating infection sites. in contrast, parasite devel ... | 1993 | 8291210 |
| 'broken backs' in koi carp (cyprinus carpio) following lightning strike. | 1993 | 8296436 | |
| molecular cloning and sequencing of a carp cdna encoding mitogen-activated protein kinase kinase. | we have cloned a full-length cdna encoding the mitogen-activated protein kinase kinase (mkk) from a carp liver cdna library. the cdna contains 1970 bp with a single open reading frame encoding a 397 amino acid protein. by comparing with known mkk sequences from other species, carp mkk is 78%, 80%, 76% and 58% identical to rat mkk1, rat mkk2, xenopus mkk and drosophila mkk. | 1994 | 8312367 |
| the occurrence of sphaerospora renicola k-stages in the choroidal rete mirabile of the common carp. | it is known from earlier studies that the so-called k-protozoa, the late presporogonic stages of sphaerospora renicola, a parasite causing renal sphaerosporosis in common carp (cyprinus carpio), accumulate in the swimbladder wall and give rise to the disease called myxosporean swimbladder inflammation. the present study indicates that in the case of intensive infection these parasites also occur in the rete mirabile of the choroid of the eye, causing haemorrhages and necrosis. the results sugges ... | 1993 | 8314174 |
| purification and properties of an indian major carp (cirrhinus mrigala, ham.) pituitary thyrotropin. | thyroid stimulating hormone (tsh) was isolated and purified from the pituitaries of indian major carp, cirrhinus mrigala, with the help of a sensitive in vitro bioassay system, based on incubating isolated thyroid follicles from murrel, channa gachua. in this assay, addition of test material containing tsh increased thyroxine (t4) release into the medium which was then measured by radioimmunoassay (ria). tsh activity was eluted as one major peak on sephadex g-100 gel filtration (peak i), which a ... | 1993 | 8319876 |
| a coccidian (apicomplexa: eimeriidae) with extracytoplasmally located stages in the kidney tubules of golden carp (carassius auratus gibelio l.) (cyprinidae). | numerous coccidian stages were found in the kidney tubules of the golden carp (carassius auratus gibelio). the merogonial and gamogonial stages were localized extracytoplasmally in the microvillous region of the epithelial cells. the host-parasite interface consisted of i) a large area where the parasite was separated from the host cytoplasm by the parasitophorous vacuole membrane only, and ii) a zone of multiple fusions of the host cell membrane investing the parasite to the neighbouring microv ... | 1993 | 8325562 |
| expansion of genes that encode mhc class i molecules in cyprinid fishes. | we have analyzed the genomic genes and the mrna that encode mhc class i molecules in cyprinid fishes, namely, the ginbuna crucian carp (carassius auratus langsdorfii) and the carp (cyprinus carpio). the presence of several distinct class i genes was revealed in the genome of cyprinid fishes, and distinct class i genes that are expressed in carp cells were identified. the sequences of the alpha 3 domain of these class i genes are relatively well conserved, whereas the sequences of the respective ... | 1993 | 8326125 |
| effects of inoculum route and dose on the immune response of common carp, cyprinus carpio to the blood parasite, trypanoplasma borreli. | carp were inoculated intramuscularly (im) or intraperitoneally (ip) with different doses of live trypanoplasma borreli. prepatent period was shortened by using the im route and by increasing the dose. haematocrit was reduced following im inoculation with 9.5 x 10(5) parasites. most fish recovered between 8 and 13 weeks post-inoculation (wpi). neither parasitaemia nor anaemia was detected in any recovered fish following homologous challenge. an enzyme-linked immunosorbent assay was developed to q ... | 1993 | 8333145 |
| cyclic nucleotide-activated channels in carp olfactory receptor cells. | when applied from the cytoplasmic side, cyclic 3',5'-adenosine and guanosine monophosphates reversibly increased the ion permeability of inside-out patches of carp olfactory neuron plasma membrane. the camp (cgmp)-induced permeability via camp (cgmp) concentration was fitted by hill's equation with the exponents of 1.07 +/- 0.15 (1.12 +/- 0.05) and ec50 = 1.3 +/- 0.6 microm (0.9 +/- 0.3 microm). substitution of nacl in the bathing solution by chlorides of other alkali metals resulted in a slight ... | 1993 | 8334139 |
| regulation of expression of transgenes in developing fish. | the transcriptional regulatory elements of the beta-actin gene of carp (cyprinus carpio) have been examined in zebrafish and goldfish harbouring transgenes. the high sequence conservation of the putative regulatory elements in the beta-actin genes of animals suggested that their function would be conserved, so that transgenic constructs with the same transcriptional control elements would promote similar levels of transgene expression in different species of transgenic animals. to test this assu ... | 1993 | 8353534 |
| expression and inheritance of rsvltr-rtgh1 complementary dna in the transgenic common carp, cyprinus carpio. | transgenic common carp, cyprinus carpio, possessing the long terminal repeat (ltr) sequence of avian rous sarcoma virus (rsv) fused to the rainbow trout (rt) growth hormone (gh1) complementary dna (cdna) were produced by microinjection. initial studies showed that the transgenic common carp transmitted the foreign dna to a significant fraction of their progeny in three of four crosses of transgenic males with control females. these progeny grew 20 to 40% faster than their nontransgenic full sibl ... | 1993 | 8364692 |
| a pulsed photolysis procedure for determining oxygen equilibrium parameters of low-affinity noncooperative hemoglobins. | it is often very difficult to obtain precise values for oxygen equilibrium constants for low-affinity noncooperative hemoglobins owing to the ease with which they are oxidized and denatured with even the most gentle stirring or agitation. we have developed a procedure that eliminates this problem by maintaining the hemoglobin as hbco except during the brief time it is present as hb, and hbo2 forms following photolysis. oxygen is continuously removed by an enzyme system. the solution is photolyze ... | 1993 | 8367536 |
| vimentin persists in the mature glia of fish brain. | adult carp brains were processed for immunohistochemical staining against vimentin and glial fibrillary acidic protein. the shape, density and distribution of immunopositive structures were found to be very similar with both types of antibody. thus, it appears that vimentin, the cytoskeletal protein typical for immature glia in the early phase of ontogenesis in mammals can also be demonstrated in the adult glia of the phylogenetically older fish brain. | 1993 | 8369799 |
| organochlorine pesticides and polychlorinated biphenyl congeners in commercial fish from the great lakes. | fillets from 11 species of commercial fish from the great lakes were analyzed for residues of 39 polychlorinated biphenyl (pcb) congeners and 24 other organochlorine compounds. eel and trout contained the highest amount of pcb (753 and 633 ppb wet weight, respectively) and other organochlorines (607 and 1404 ppb, respectively); perch and carp contained the lowest residues. the penta- and hexachlorinated pcbs were the major congeners in all species except whitefish, in which the tetrachlorinated ... | 2013 | 8374320 |
| differences in mucus and serum immunoglobulin of carp (cyprinus carpio l.). | electrophoretic analysis did not reveal clear differences between skin mucus and serum immunoglobulin (ig) of carp. the majority of both igs were tetrameric (+/- 760 kda) and composed of 25 kda light (l) chains and 70 kda heavy (h) chains, but dimeric and monomeric forms were found as well. monoclonal antibody (mab) wci 12 produced from serum ig appeared to react with the h chain of both molecules. after immunisation of mice with purified mucus ig, mabs could be selected that were reactive with ... | 2003 | 8375566 |
| cloning and characteristics of fish glial fibrillary acidic protein: implications for optic nerve regeneration. | mammalian central nervous system neurons do not regenerate after axonal injury, unlike their counterparts in fish and amphibians. after axonal injury, glial cells in mammals do not support regrowth of axons, while in fish they support the regeneration process. controversy exists as to whether or not the intact fish optic nerve expresses glial fibrillary acidic protein, a well-known marker for mature astrocytes, and thus whether its astrocytes differ in this respect from those of the brain and sp ... | 1993 | 8376626 |
| toxic effects of industrial effluents on hatchability and viability of cyprinus carpio eggs. | 1993 | 8387839 | |
| the phylogenetic position of the zebrafish (danio rerio), a model system in developmental biology: an invitation to the comparative method. | the zebrafish, danio (brachydanio) rerio, has become one of the most widely studied model systems in developmental biology. we present a dna-based phylogeny of zebrafish and other species of the genus danio, and the genera rasbora, puntius and cyprinus. homologous regions of the large (16s) mitochondrial ribosomal rna gene were amplified by the polymerase chain reaction and directly sequenced. the phylogeny revealed: (i) the zebrafish, danio (brachydanio) rerio, is identical in its 16s sequence ... | 1993 | 8394584 |
| efficiency of introns from various origins in fish cells. | several vectors containing (1) regulatory regions from rous sarcoma virus (rsv), human cytomegalovirus (cmv), and herpes simplex thymidine kinase (tk); (2) introns from early or late sv40 genes and from trout growth hormone gene (tgh); (3) chloramphenicol acetyltransferase gene (cat); and (4) transcription terminators from sv40 were transfected into carp epc cells, salmon chse cells, tilapia to2 cells, quail qt6 cells, and hamster cho cells. cat activity was measured in extracts from several cel ... | 1993 | 8395951 |
| hypo-osmotic shock induces an osmolality-dependent permeabilization and structural changes in the membrane of carp sperm. | we carried out spectrofluorimetric and flow cytometric measurements to investigate the effect of hypo-osmotic shock on cell membranes of common carp sperm. the time course of the permeability of the sperm cell membrane, as monitored by dna-related propidium iodide (pi) fluorescence, was followed for 30 min after dilution of semen in hypo-osmotic environments of different ionic strengths. spectrofluorimetric measurements indicated a continuous increase in the total pi emission intensity of a sper ... | 1993 | 8419464 |
| acute toxicity of selected organophosphorus pesticides to cyprinus carpio and barilius vagra. | the acute toxicity of two organophosphorus (op) pesticides, diazinon and malathion to cyprinus carpio and barilius vagra was determined. mortalities and lc50-96 hr values for b. vagra and c. carpio were variable. both juvenile common carp and b. vagra were extremely sensitive to diazinon. adult b. vagra were more sensitive to malathion than were juvenile common carp. | 1993 | 8426062 |