Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| tracking the fate of iron in early development of human blood flukes. | iron (fe) is an important trace element found in nearly all organisms, and is used as a cofactor in many biological reactions. one role for fe in some invertebrates is in stabilization of extracellular matrices. the human blood fluke, schistosoma japonicum, is responsible for significant human disease in developing and tropical nations. disease in humans arises from host immunological reaction to parasite eggs that lodge in tissues. schistosomes require fe for development in their hosts, and sto ... | 2007 | 17556009 |
| field comparison of immunodiagnostic and parasitological techniques for the detection of schistosomiasis japonica in the people's republic of china. | a total of 1,811 individuals from two villages located in the areas of china endemic for schistosoma japonicum were analyzed by the kato-katz parasitologic examination, indirect hemagglutination assay (iha), and enzyme-linked immunosorbent assay (elisa). statistical analysis of the results showed the kappa indices ranged from 0.106 to 0.234 between iha and the stool examination and ranged from 0.037 to 0.134 between elisa and the fecal examination. the sensitivity value of the iha was 83.7% in v ... | 2007 | 17556625 |
| making the most of mitochondrial genomes--markers for phylogeny, molecular ecology and barcodes in schistosoma (platyhelminthes: digenea). | an increasing number of complete sequences of mitochondrial (mt) genomes provides the opportunity to optimise the choice of molecular markers for phylogenetic and ecological studies. this is particularly the case where mt genomes from closely related taxa have been sequenced; e.g., within schistosoma. these blood flukes include species that are the causative agents of schistosomiasis, where there has been a need to optimise markers for species and strain recognition. for many phylogenetic and po ... | 2007 | 17570370 |
| vaccination of goats with 31 kda and 32 kda schistosoma japonicum antigens by dna priming and protein boosting. | two schistosoma japonicum vaccine candidate antigens sj 31 and sj 32, which have shown particular promise to induce protective immunity in mice, were used to immunize goats by using a dna priming-protein boosting strategy in present work. dna vaccine formulations of the two antigens (vrsj31 and vrsj32) were produced and injected intramuscularly twice at a 2-week interval and then recombinant proteins (rsj31 and rsj32) together with freund complete adjuvant (fca) were used to boost the goats. the ... | 2007 | 17571462 |
| [cloning, expression and characterization of a gene encoding signal transduction protein wnt4 of schistosoma japonicum]. | wnt proteins together with their downstream effectors forms a set of important signal pathways. the wnt signal pathway is important in a wide variety of development processes including cell growth, cell differentiation, cell polarity and apoptosis. wnt4 is a key regulator of gonadal differentiation in humans and mice, playing a pivotal role in early embryogenesis. with race technique based on a est identified in our lab, a novel gene including a complete open reading frame was cloned and named s ... | 2007 | 17577981 |
| gene discovery for the carcinogenic human liver fluke, opisthorchis viverrini. | cholangiocarcinoma (cca)--cancer of the bile ducts--is associated with chronic infection with the liver fluke, opisthorchis viverrini. despite being the only eukaryote that is designated as a 'class i carcinogen' by the international agency for research on cancer, little is known about its genome. | 2007 | 17587442 |
| [a comparison of therapeutic outcomes in liver fibrosis of murine schistosomiasis japonica with interferon-alpha and interferon-gamma]. | 2007 | 17594819 | |
| metal binding ability of glutathione transferases conserved between two animal species, the vanadium-rich ascidian ascidia sydneiensis samea and the schistosome schistosoma japonicum. | glutathione transferases (gsts) are multifunctional enzymes found in many organisms. we recently identified vanadium-binding gsts, designated asgsts, from the vanadium-rich ascidian, ascidia sydneiensis samea. in this study, the metal-selectivity of asgst-i was investigated. immobilized metal ion affinity chromatography (imac) analysis revealed that asgst-i binds to v(iv), fe(iii), and cu(ii) with high affinity in the following order cu(ii)>v(iv)>fe(iii), and to co(ii), ni(ii), and zn(ii) with l ... | 2007 | 17602839 |
| the schistosoma mansoni transcriptome: an update. | large scale est sequencing projects have been carried out for schistosoma mansoni and schistosoma japonicum. this update will briefly review the most recent accomplishments in the area and discuss the use of est data for the purposes of gene discovery, gene model development, genome annotation and snp analysis. in addition, the use of ests for studying other features of the transcriptome such as splice site and transcription initiation variants will be discussed as well as approaches to assignin ... | 2007 | 17624328 |
| prevalence of schistosoma japonicum infection among animals in fifty villages of samar province, the philippines. | in the philippines, there is a need to understand the contribution of different domestic and wild animals in transmitting schistosoma japonicum infection to humans better. the current study describes variation in animal s. japonicum prevalence across 50 endemic villages of samar province, the philippines. a total of 50 villages were selected, 25 with predominantly rain-fed farms and 25 with some irrigation system. at least 35 cats, dogs, pigs, and water buffaloes each were randomly selected and ... | 2007 | 17627431 |
| [effect of n-acetylcysteine on the egg granuloma in hepatic tissue of mice with schistosomosis japonica]. | to study the effect of n-acetylcysteine (nac) on the egg granuloma in hepatic tissue of mice infected with schistosoma japonicum. | 2007 | 17633826 |
| [observation on the change of anti-s. japonicum antibody level in population migrated from outside embankment to new town]. | to detect the change of the anti-s. japonicum antibody level after people migrated from outside embankment to newly established town. | 2007 | 17633828 |
| assessment of the age-specific disability weight of chronic schistosomiasis japonica. | to estimate the age-specific disability weight of chronic schistosomiasis japonica in china. | 2007 | 17639243 |
| [cloning and fusion expression of adenosine deaminase of schistosoma japonicum]. | to clone and express a new protein adenosine deaminase of schistosoma japonicum (sjada). | 2007 | 17639691 |
| [secreted expression of signaling protein 14-3-3 of schistosoma japonicum in pichia pastoris system with primary evaluation on its antigenicity]. | to express signaling protein sj14-3-3 in pichia pastoris and compare its antigenicity with prokaryotic expression one. | 2007 | 17639692 |
| [studies on mechanism of protective immunity against infection of schistosoma japonicum induced by sj26 gene transfected dendritic cell]. | to explore the mechanism of protective immunity against schistosoma japonicum infection induced by sj26 gene transfected dendritic cell (dc). | 2007 | 17639693 |
| schistosoma mansoni genome: closing in on a final gene set. | the schistosoma mansoni genome sequencing consortium has recently released the latest versions of the genome assembly as well as an automated preliminary gene structure annotation. the combined datasets constitute a vast resource for researchers to exploit in a variety of post-genomic studies with an emphasis of transcriptomic and proteomic tools. here we present an innovative method used for combining diverse sources of evidence including ab initio gene predictions, protein and transcript seque ... | 2007 | 17643433 |
| surgical treatment of epilepsy with chronic cerebral granuloma caused by schistosoma japonicum. | to study the surgical treatment of epilepsy with cerebral granuloma caused by schistosoma japonicum. | 2008 | 17662065 |
| lysophospholipase from the human blood fluke, schistosoma japonicum. | given the unusual nature of the schistosome surface (a highly unusual lipid bi-layer) and the central role of the schistosome tegument in host-parasite relations, an enhanced understanding of the lipid biochemistry of the schistosome surface can be expected to provide new insights into schistosome pathogenesis and lead to new interventions. | 2008 | 17709268 |
| helminth infections and risk factor analysis among residents in eryuan county, yunnan province, china. | whilst infections with soil-transmitted helminths are common across china, the public-health significance of schistosoma japonicum and food-borne helminths is more focalized. only few studies have investigated the local epidemiology of helminth infections in rural china, including risk factor analysis. we collected stool and blood samples from 3220 individuals, aged 5-88 years, from 35 randomly selected villages in eryuan county, yunnan province, china. stool samples were subjected to the kato-k ... | 2007 | 17719553 |
| praziquantel and the benzodiazepine ro 11-3128 do not compete for the same binding sites in schistosomes. | the benzodiazepine ro 11-3128 (methyl-clonazepam) presents several similarities with praziquantel with regard to its anti-schistosomal mode of action, since both drugs cause spastic paralysis, calcium influx and tegumental disruption in the parasites. in order to know whether the two compounds share the same binding sites in the schistosomes, we performed in vivo and in vitro competition experiments. we took advantage of the fact that ro 11-3128 is active against immature schistosoma mansoni (wh ... | 2008 | 17767796 |
| [impact of people's social behavior on schistosomiasis transmission in the three gorges reservoir area]. | to investigate the changes in natural ecology and social environment after the construction of the dam in the three gorges reservoir area, and to understand the social behavior status and education backgrounds of the local residents, and to analyze the potential impact of these factors on schistosomiasis spreading. | 2007 | 17767880 |
| prevalence, intensity and associated morbidity of schistosoma japonicum infection in the dongting lake region, china. | to determine the prevalence and intensity of schistosoma japonicum infection and associated morbidity, and to estimate the infected human and buffalo populations in the dongting lake region, hunan province, china. | 2007 | 17768500 |
| erratum. | in gina kolata's article "avoiding the schistosome's tricks" (research news, 18 jan., p. 285), schistosoma mansoni and s. japonicum were inadvertently interchanged in the first full paragraph of the first column on page 286. schistosoma japonicum lives in the mesenteric veins of the upper (small) intestine, and s. mansoni lives in the mesenteric veins of the lower (large) intestine. | 1985 | 17790209 |
| the habitat of the snail host of schistosoma japonicum in the philippines. | 1946 | 17815560 | |
| intrahepatic cholangiocarcinoma with old infestation of schistosoma japonicum: report of a case. | we report a rare case of intrahepatic cholangiocarcinoma associated with old infestation of schistosoma japonicum. the patient was a 76-year-old japanese man who had lived his childhood in an endemic area of this parasite. he presented with jaundice and computed tomography showed a 4-cm, hypodense tumor in segment viii of the liver. microscopically, the resected mass was composed of well-differentiated adenocarcinoma cells. fibrosis and inflammation were seen around the dilated peripheral portal ... | 2007 | 17879045 |
| diagnosis of schistosomiasis japonica infection coincident with hepatocellular carcinoma by fine-needle aspiration. | schistosomiasis japonica is not an endemic parasite in taiwan. previously, there have been no primarily infected cases reported with the exception of chinese war veterans who had immigrated from mainland china. we reported a 79-year-old patient who was diagnosed as having non-b, non-c liver cirrhosis complicated with a 1.2 x 1.2 x 1.2 cm liver tumor in the s5 of the liver. for a definitive diagnosis, we performed ultrasonographic-guided fine needle (21 gauge) aspiration biopsy. in the smear of l ... | 2007 | 17924411 |
| characterization of a partially protective b-cell epitope within the 62 kda antigen of schistosoma japonicum. | approximately 200 million people worldwide currently suffer from schistosomiasis, one of the most important human parasitic diseases. although an established infection can be treated with anthelminthics and praziquantel, vaccination would be the ideal method for integral control of schistosomiasis. schistosoma mansoni irv-5, recommended as a vaccine candidate by the world health organization/special programme for research and training in tropical diseases, produced high protection in animal mode ... | 2007 | 17928926 |
| differential use of protease families for invasion by schistosome cercariae. | schistosomes are parasitic platyhelminths (flatworms) of birds and mammals. as a parasitic disease of humans, schistosomiasis ranks second only to malaria in global importance. schistosome larvae (cercariae) must invade and penetrate skin as an initial step to successful infection of the vertebrate host. proteolytic enzymes secreted from the acetabular glands of cercariae contribute significantly to the invasion process. in this comparative study, we analyzed protease activities secreted by cerc ... | 2008 | 17936488 |
| individual and village-level study of water contact patterns and schistosoma japonicum infection in mountainous rural china. | to describe the exposure patterns related to schistosomiasis transmission in 10 villages in rural xichang county, sichuan, china. | 2007 | 17956502 |
| immunization of mice with cells from juvenile worms of schistosoma japonicum provides immunoprotection against schistosomiasis. | to validate the protective efficacy against schistosomiasis by immunization with cells from juvenile schistosoma japonicum in a murine model and to analyze possible factors related to protection, in this study, two independent repeated vaccination trials were performed. after three subcutaneous vaccinations, in trial one, in the absence of adjuvant, primary juvenile worm cells (pjcs) from s. japonicum induced remarkable average reductions in worm burden (54.3%), liver eggs per gram (lepg) load ( ... | 2007 | 17973094 |
| artemisinins and synthetic trioxolanes in the treatment of helminth infections. | helminthiases, including schistosomiasis and food-borne trematodiasis, affect millions of people. just a few drugs are used for the treatment and control of these diseases. we review recent in-vitro and in-vivo observations with the artemisinins and synthetic trioxolanes against major trematode infections, update clinical findings, and discuss the potential impact that artemisinin-based combination therapy might have on trematode infections in settings where malaria and helminthiases are co-ende ... | 2007 | 17975411 |
| a cluster-randomized bovine intervention trial against schistosoma japonicum in the people's republic of china: design and baseline results. | we describe the design and report baseline results of a cluster-randomized intervention to determine the importance of bovines for schistosoma japonicum transmission in southern china. the study involves four matched village pairs in hunan and jiangxi provinces, with a village within each pair randomly selected as intervention (human and bovine praziquantel treatment) or control (human praziquantel treatment only). total study population prevalences at baseline were 12.4% (n = 5,390) and 15.2% ( ... | 2007 | 17984344 |
| [effect of schistosome ova on trinitrobenzenesulfonic acid induced colitis in mice]. | to investigate the effects of intraperitoneal injected schistosome ova on tnbs-induced colitis and on the intestinal tlr4 expression in mice. | 2007 | 17988539 |
| transgenesis of schistosomes: approaches employing mobile genetic elements. | draft genome sequences for schistosoma mansoni and schistosoma japonicum are now available. however, the identity and importance of most schistosome genes have yet to be determined. recently, progress has been made towards the genetic manipulation and transgenesis of schistosomes. both loss-of-function and gain-of-function approaches appear to be feasible in schistosomes based on findings described in the past 5 years. this review focuses on reports of schistosome transgenesis, specifically thos ... | 2008 | 17991304 |
| application of recombinant sjc26gst for serodiagnosis of schistosoma japonicum infection in water buffalo (bos buffelus). | schistosomiasis japonica is currently the most serious parasitic disease in mainland china and it is estimated that several million people are infected. furthermore, it is also responsible for the deaths of many domestic animals. in order to establish an effective diagnostic method, the gene encoding sjc26gst was cloned and expressed in escherichia coli as a fusion protein with his-tag. the purified resjc26gst was used as an antigen for an enzyme-linked immunosorbent assay (elisa) and for immuno ... | 2007 | 17997225 |
| bursts and horizontal evolution of dna transposons in the speciation of pseudotetraploid salmonids. | several genome duplications have occurred in the evolutionary history of teleost fish. in returning to a stable diploid state, the polyploid genome reorganized, and large portions are lost, while the fish lines evolved to numerous species. large scale transposon movement has been postulated to play an important role in the genome reorganization process. we analyzed the dna sequence of several large loci in salmo salar and other species for the presence of dna transposon families. | 2007 | 18021408 |
| effects of the praziquantel-based control of schistosomiasis japonica in china. | in 2000-2002, the effects of the praziquantel-based control of schistosoma japonicum (in which each subject found infected in the october of each year is given a single dose of the drug) were examined at 20 or 21 surveillance sites across china. the pre- and post-treatment prevalences and intensities of infection were compared at each site and after pooling using a random-effects method. one year after treatment, in the communities that had the higher prevalences of infection (> 10%) before trea ... | 2007 | 18028731 |
| real-time pcr for detection of low intensity schistosoma japonicum infections in a pig model. | decades of successful schistosoma japonicum control have increased the interest in how to diagnose low intensity infections. a real-time pcr assay targeting the mitochondrial nadh dehydrogenase i gene in s. japonicum was evaluated in infected pigs with very low egg output. six out of 12 s. japonicum infected pigs were treated with praziquantel 8 weeks after infection and all pigs were followed for 16 weeks post-infection. one commercial and one non-commercial extraction method were evaluated in ... | 2008 | 18036505 |
| [effectiveness of routinely used assays for the diagnosis of schistosomiasis japonica in the field]. | to evaluate the effectiveness of routinely used assays for schistosomiasis diagnosis in the field. | 2007 | 18038771 |
| [potential risks for transmission of schistosomiasis caused by mobile population in shanghai]. | to understand the potential risk for schistosomiasis transmission caused by introduction of infection source from mobile population in shanghai. | 2007 | 18038772 |
| [production and identification of chicken egg yolk antibodies against soluble egg antigen of schistosoma japonicum]. | to produce and purify egg yolk immunoglobulin against soluble egg antigen (sea) of schistosoma japonicum, and evaluate its specificity and sensitivity. | 2007 | 18038775 |
| [bioinformatics analysis for the structure and function of lactate dehydrogenase from schistosoma japonicum]. | to predict the structure and function of sjldh using bioinformatics method. | 2007 | 18038778 |
| [development and identification of the multiple b cell epitope antigens of schistosoma japonicum]. | to develop multiple b cell epitope antigens of schistosoma japonicum and evaluate their antigenicity. | 2007 | 18038797 |
| analysis of the 5q31 33 locus shows an association between single nucleotide polymorphism variants in the il-5 gene and symptomatic infection with the human blood fluke, schistosoma japonicum. | genetic studies of human susceptibility to schistosoma (blood fluke) infections have previously identified a genetic locus determining infection intensity with the african species, schistosoma mansoni, in the 5q31-33 region of the human genome that is known to contain the th2 immune response cluster, including the genes encoding the il-4, il-5, and il-13 cytokines. these cytokines are key players in inflammatory immune responses and have previously been implicated in human susceptibility to infe ... | 2007 | 18056382 |
| comparative genomics of human-like schistosoma japonicum genes indicates a putative mechanism for host-parasite relationship. | schistosoma japonicum causes schistosomiasis in humans and livestock in the asia-pacific region. we assembled more than 43,700 s. japonicum expressed sequence tags and conducted comparative genomic analyses between s. japonicum and its human host. some schistosome genes showed exceptionally high similarity in nucleotide sequence to their human homologues, of which five exhibited anomalous phylogeny and human codon usage bias. the most plausible explanation for their presence is horizontal gene t ... | 2008 | 18083328 |
| the action of miracil in schistosoma japonicum infections in laboratory animals. | 1948 | 18123796 | |
| bionomics of oncomelania fausti? the intermediate host of schistosoma japonicum. | 1949 | 18126778 | |
| the molluscan intermediate host and schistosomiasis japonica; observations on the conditions governing the hatching of the eggs of schistosoma japonicum. | 1949 | 18129029 | |
| asymptomatic schistosoma japonicum infection associated with rectal carcinoma; report of a case. | 1949 | 18138920 | |
| the control of schistosomiasis japonica; studies on the penetration of various types of unimpregnated uniform cloth by cercariae of schistosoma japonicum. | 1949 | 18141393 | |
| a model for the prediction of oncomelania hupensis in the lake and marshland regions, china. | a model has been developed for predicting the density of oncomelania hupensis, the intermediate host snail of schistosoma japonicum. the model takes into account different environmental factors, including elevation, air and soil temperature, type of vegetation, mean height of preponderant vegetation and soil humidity. deviance and akaike information criteria were used to determine the best model fits. model diagnostics and internal and external validations of model efficiency were also performed ... | 2008 | 18187358 |
| current status of vaccines for schistosomiasis. | schistosomiasis, caused by trematode blood flukes of the genus schistosoma, is recognized as the most important human helminth infection in terms of morbidity and mortality. infection follows direct contact with freshwater harboring free-swimming larval (cercaria) forms of the parasite. despite the existence of the highly effective antischistosome drug praziquantel (pzq), schistosomiasis is spreading into new areas, and although it is the cornerstone of current control programs, pzq chemotherapy ... | 2008 | 18202444 |
| prevalence of schistosoma japonicum infection of oncomelania quadrasi snail colonies in 50 irrigated and rain-fed villages of samar province, the philippines. | a cross-sectional survey of oncomelania quadrasi, the intermediate host for schistosoma japonicum, was conducted between 2004 and 2005 in 50 villages of the province of samar, the philippines. the villages were classified as rain-fed (25) or with some man-made irrigation system (25). the primary objective was to identify all snail colony sites in the 50 villages and to compare snail population density and s. japonicum infection prevalence between the two types of villages. the presence of snail ... | 2008 | 18207119 |
| soluble egg antigen from schistosoma japonicum modulates the progression of chronic progressive experimental autoimmune encephalomyelitis via th2-shift response. | soluble egg antigen (sea) is strongly antigenic and inherently induces th2-biased immune responses. in this study, we tested whether sea from schistosoma japonicum is able to prevent experimental autoimmune encephalomyelitis (eae) induced by mog(35-55) peptide, an established animal model of multiple sclerosis (ms). intraperitoneal administration with sea before eae induction and in the preclinical phase after eae induction successfully ameliorated the severity and progression of eae on mice com ... | 2008 | 18207251 |
| cerebral schistosomiasis japonica without gastrointestinal system involvement. | schistosoma japonicum is the most widespread schistosoma in the world. although gastrointestinal system involvement with s japonicum appears to be considerably common, cerebral schistosomiasis is not frequent. cerebral schistosomiasis japonica intestinal and hepatosplenic involvement is more rare. we collected 2 cases of cerebral schistosomiasis identified by pathological diagnosis, lacking extracranial involvement. in addition, one of them had multiple lesions, which was also rare. | 2009 | 18207555 |
| multi-host transmission dynamics of schistosoma japonicum in samar province, the philippines. | among the 6.7 million people living in areas of the philippines where infection with schistosoma japonicum is considered endemic, even within small geographical areas levels of infection vary considerably. in general, the ecological drivers of this variability are not well described. unlike other schistosomes, s. japonicum is known to infect several mammalian hosts. however, the relative contribution of different hosts to the transmission cycle is not well understood. here, we characterize the t ... | 2008 | 18215106 |
| model-based insights into multi-host transmission and control of schistosomiasis. | 2008 | 18215110 | |
| construction and expression of dna vaccine pires-sj97-sj14-sj26 and its immunogenicity in mice. | to find a new preventive strategy for the infection of schistosoma japonica, plasmid pires-sj97-sj14-sj26 that contains fatty binding protein (sj14), gst (sj26) and paramyocin (sj97) that are expressed on the membrane, was constructed. rt-pcr was used to detect the expression of sj14 mrna, sj26 mrna and sj97 mrna in the hela cells, the indirect immunofluorescent test was employed for the detection of the expression of trans-membrane sj26 after the plasmid was transfected into hela cells. fifty b ... | 2007 | 18231727 |
| the effects of t cell deficiency on the development of worms and granuloma formation in mice infected with schistosoma japonicum. | it is widely accepted that the immune response of the host attacks the parasite and the parasite appears to develop strategies to evade the assault. however, there is increasing evidence that the development of a parasite may be also positively influenced by the immune response of host. in this paper, we explore the effects of t cell deficiency on the development of the worms and granuloma formation in mice infected with cercariae of schistosoma japonicum. t cell-deficient (nude) mice supported ... | 2008 | 18246371 |
| where snails no longer fear to tread. | 2008 | 18256408 | |
| potential impact of climate change on schistosomiasis transmission in china. | appraisal of the present and future impact of climate change and climate variability on the transmission of infectious diseases is a complex but pressing public health issue. we developed a biology-driven model to assess the potential impact of rising temperature on the transmission of schistosomiasis in china. we found a temperature threshold of 15.4 degrees c for development of schistosoma japonicum within the intermediate host snail (i.e., oncomelania hupensis), and a temperature of 5.8 degre ... | 2008 | 18256410 |
| epidemiology of schistosomiasis in the people's republic of china, 2004. | results from the third nationwide cluster sampling survey on the epidemiology of schistosomiasis in the people's republic of china, conducted by the ministry of health in 2004, are presented. a stratified cluster random sampling technique was used, and 239 villages were selected in 7 provinces where schistosoma japonicum remains endemic. a total of 250,987 residents 6-65 years of age were included in the survey. estimated prevalence rates in the provinces of hunan, hubei, jiangxi, anhui, yunnan, ... | 2007 | 18257989 |
| i-stod: a new standardization method for analysing indirect-elisa results of a schistosomiasis field study. | variability among samples analysed using the same elisa protocol generates ambiguity in deciding which assay best quantifies the protein concentration. in this study, we propose a standardization method, called i-stod (improved standardization method for optical density), for the transformation of od values on different plates into relative concentrations of the antibody levels being assessed. we derived an equation relating od values of different test samples to antibody levels according to the ... | 2008 | 18261254 |
| comparative real-time pcr and enzyme analysis of selected gender-associated molecules in schistosoma japonicum. | schistosomes are complex parasitic helminths with discrete life-cycle stages, adapted for survival in their mammalian and snail hosts and the external aquatic environment. recently, we described the fabrication and use of a microarray to investigate gender-specific transcription in schistosoma japonicum. to address transcriptional differences, 8 gender-associated gene transcripts identified previously by the microarray analysis were selected for further study. first, differential transcription p ... | 2008 | 18294425 |
| an integrated approach to identify distribution of oncomelania hupensis, the intermediate host of schistosoma japonicum, in a mountainous region in china. | the aim of this study is to better understand ecological variability related to the distribution of oncomelania hupensis, the snail intermediate host of schistosoma japonicum, and predict the spatial distribution of o. hupensis at the local scale in order to develop a more effective control strategy for schistosomiasis in the hilly and mountainous regions of china. a two-pronged approach was applied in this study consisting of a landscape pattern analysis complemented with bayesian spatial model ... | 2008 | 18294641 |
| t cell epitope-based peptide-dna dual vaccine induces protective immunity against schistosoma japonicum infection in c57bl/6j mice. | schistosomiasis is a major public health problem that primarily affects developing countries. although schistosomicidal drugs exist, the development of an efficacious vaccine would potentially be the most powerful means of controlling this disease. previous studies have shown that vaccination with selected protective epitopes successfully induced partial protection and/or reduced female fecundity in animal models. thus, we investigated whether the t cell epitope p5 from the host-interactive tegu ... | 2008 | 18316223 |
| decision-model estimation of the age-specific disability weight for schistosomiasis japonica: a systematic review of the literature. | schistosomiasis is among the most prevalent parasitic infections worldwide. however, current global burden of disease (gbd) disability-adjusted life year estimates indicate that its population-level impact is negligible. recent studies suggest that gbd methodologies may significantly underestimate the burden of parasitic diseases, including schistosomiasis. furthermore, strain-specific disability weights have not been established for schistosomiasis, and the magnitude of human disease burden due ... | 2008 | 18320018 |
| b cell response is required for granuloma formation in the early infection of schistosoma japonicum. | schistosoma egg-induced liver granuloma is a dynamic inflammatory reaction that results from complex immune responses to the infection. however, the role of b cells in inflammatory granuloma development is not yet fully understood. we report here that b cell function is required for s. japonicum egg-induced granuloma pathology in early infection. both obf-1 knockout mice and micromt mice develop severely reduced hepatic granulomas at five weeks post-infection compared to their wild-type counterp ... | 2008 | 18320044 |
| characterization of a profilin-like protein from schistosoma japonicum, a potential new vaccine candidate. | the tegumental membrane of platyhelminth parasites is of crucial importance for modulation of the host response and parasite survival. a complementary deoxyribonucleic acid (cdna) containing an open reading frame of 390 bp, which encodes a profilin-like tegumental protein with a theoretical isoelectric point of 6.02 and a molecular weight of 14.42 kda, had been identified by bioinformatic analysis. the coding region of the cdna was cloned into the prokaryotic expression vector pgex-4t-1 and expr ... | 2008 | 18320225 |
| the unacknowledged impact of chronic schistosomiasis. | to summarize new knowledge on the range of disease due to chronic schistosomiasis and examine the debilitating burden of both light and heavy infection; to outline goals of disease prevention, including current age-targeted strategies and more extended programmes aimed at preventing transmission. | 2008 | 18322031 |
| expression, immunolocalization and serodiagnostic value of a myophilin-like protein from schistosoma japonicum. | the cdna of a schistosoma japonicum myophilin-like protein was cloned, sequenced, and expressed in escherichia coli as a recombined protein (rsj myophilin-like protein), and the protein was purified by affinity chromatography. the deduced amino acid sequences of the sj myophilin-like protein showed significant homology to myophilin, calponin, np22 and mp20. northern blot and rt-pcr analyzes revealed expression of the sj myophilin-like protein mrna in eggs, sporocysts, cercariae, hepatic schistos ... | 2008 | 18329020 |
| the protective efficacy against schistosoma japonicum infection by immunization with dna vaccine and levamisole as adjuvant in mice. | levamisole (lms) as an adjuvant enhances cell-mediated immunity in dna vaccination; we investigated the efficacy and liver immunopathology alleviation of a dna vaccine, vr1012-sjgst-32, in a lms formulation in the murine challenge model. compared to controls, the vr1012-sjgst-32 plus lms can reduce worm and egg burdens, as well as, immunopathological complications associated chronic inflammation significantly in liver, which were apparently associated with th1-type response. together, these resu ... | 2008 | 18329762 |
| evaluation of recombinant fructose-1,6-bisphosphate aldolase elisa test for the diagnosis of schistosoma japonicum in water buffaloes. | fructose-1,6-bisphosphate aldolase (fbpa) is an ubiquitous enzyme essential for glycolysis, gluconeogenesis and the calvin cycle. it has been demonstrated to induce immune responses and to be useful in the immunodiagnosis of malaria. in this study, fbpa was cloned from the adult worms of schistosoma japonicum and tested as an antigen for the diagnosis of s. japonicum infection in water buffaloes. enzyme-linked immunosorbent assay (elisa) was performed on the sera from 32 infected water buffaloes ... | 2008 | 18371996 |
| schistosomiasis in travelers and immigrants. | schistosomiasis is caused by trematode parasites of one of five species, schistosoma mansoni, schistosoma haematobium, schistosoma japonicum, schistosoma intercalatum, or schistosoma mekongi, acquired via skin contact with fresh water containing infectious larval cercariae. between 1 and 8 weeks after exposure, acute schistosomiasis presents as dermatitis, fever, fatigue, or eosinophilia. if untreated, long-term infection may cause anemia or undernutrition and may progress to liver fibrosis, por ... | 2008 | 18377815 |
| a trifunctional enzyme with glutathione s-transferase, glutathione peroxidase and superoxide dismutase activity. | superoxide dismutase (sod), glutathione peroxidase (gpx), glutathione s-transferase (gst) and glutathione reductase (gr) play crucial roles in balancing the production and decomposition of reactive oxygen species (ros) in living organisms. these enzymes act cooperatively and synergistically to scavenge ros, as not one of them can singlehandedly clear all forms of ros. in order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a schistosoma ... | 2008 | 18381079 |
| evaluation of a rapid elisa technique for detection of circulating antigens of toxoplasma gondii. | to evaluate a modified rapid elisa method for detecting cag during toxoplasma gondii infection, we analyzed the specificity and sensitivity of the elisa method by using experimental toxoplasma infection in rabbits and also tested this method in human samples including 5428 serum, 548 cerebrospinal fluid and two breast milk samples. we prepared pcab, and used it for rapid one-step sandwich elisa testing in which an incubation time in the regular elisa procedure was omitted. this method detected c ... | 2008 | 18402600 |
| pilot-scale production and characterization of paramyosin, a vaccine candidate for schistosomiasis japonica. | despite effective chemotherapy, schistosomiasis remains a major public health problem in the developing world, with at least 200 million active infections resulting in significant morbidity. rapid reinfection after treatment, accompanied by extensive residual morbidity, mandates alternative control strategies, including vaccine development. paramyosin, a myofibrillar protein found only in invertebrates, has been widely studied as a vaccine candidate for both schistosoma mansoni and schistosoma j ... | 2008 | 18426875 |
| [parasite-origin ige-dependent histamine-releasing factors in inducing histamine release from sensitized mast cells]. | to obtain the recombinant ige-dependent histamine-releasing factors of schistosoma japonicum and clonorchis sinensis (rsjhrf and rcshrf) and to study the effect of recombinant hrfs to induce histamine release from sensitized rat mast cells. | 2007 | 18441893 |
| [endemic situation of schistosomiasis at the national surveillance sites of nanjing, 2006]. | three endemic villages were selected as the national surveillance sites and schistosoma japonicum infection in residents and livestock was investigated in 2006. the positive rate of serological (iha) and stool examinations was 10.06% (307/3,053) and 0.13% (4/3,053) for local residents, 10.8% (7/65) and 0 (0/7) for moving people respectively. no infected livestock was found. | 2007 | 18441901 |
| [cloning, expression of schistosoma japonicum elastase gene and its stage-specific transcription]. | to clone, express and purify schistosoma japonicum elastase-2b gene (sjce-2b), and analyze its stage-specific transcription. and expression. | 2007 | 18441984 |
| [suppression of schistosoma japonicum eggs on tnbs-induced colitis in mice]. | to study the suppression of schistosoma japonicum eggs against the trinitrobenzene sulfonic acid (tnbs)-induced colitis in mice. | 2007 | 18441989 |
| [the effect of gossypol, praziquantel and artemether on recombinant lactate dehydrogenase from schistosoma japonicum]. | to detect the effect of gossypol, praziquantel and artemether on activity of the recombinant lactate dehydrogenase of schistosoma japonicum (rsjldh). | 2007 | 18441994 |
| [construction of phage display cdna library from adult worms of schistosoma japonicum]. | to screen protective antigen genes and construct the t7 phage display library from adult worms of schistosoma japonicum. | 2007 | 18441996 |
| [current status and prospects on candidate vaccines against schistosoma japonicum]. | great progress has been made on vaccine research for schistosomiasis, including those on immune mechanism and schistosoma genome which have made active effect to vaccine development. this paper reviews the progress on the candidate vaccine antigens including protein vaccine, dna vaccine and multivalent vaccine against schistosoma japonicum. | 2007 | 18442002 |
| schistosoma japonicum: inhibition of mago nashi gene expression by shrna-mediated rna interference. | rna interference (rnai) mediated by short interfering rna (sirna) is a powerful reverse genetics tool and holds enormous therapeutic potential for various diseases, including parasite infections. sirnas bind their complementary mrna and lead to degradation of their specific mrna targets. rnai has been widely used for functional analysis of specific genes in various cells and organisms. in this paper, we tested the potential of silencing the expression of the mago nashi gene in schistosoma japoni ... | 2008 | 18466902 |
| rosiglitazone prevents murine hepatic fibrosis induced by schistosoma japonicum. | to evaluate the effect of rosiglitazone in a murine model of liver fibrosis induced by schistosoma japonicum infection. | 2008 | 18473419 |
| routine kato-katz technique underestimates the prevalence of schistosoma japonicum: a case study in an endemic area of the people's republic of china. | there is an evidence that the kato-katz technique lacks sensitivity and may hence be an unsuitable method for the assessment of the 'real infection status' in community with low-intensity infections. in this study, six kato-katz thick smears (examination of two stool samples with three thick smears each) were used as the diagnostic 'gold' standard for estimating the prevalence of schistosoma japonicum infection and the results were compared with results based on fewer kato-katz thick smear readi ... | 2008 | 18485807 |
| molecular characterization of schistosoma japonicum tegument protein tetraspanin-2: sequence variation and possible implications for immune evasion. | members of tetraspanin family expressed on the tegument of schistosoma mansoni have been regarded as potential protective antigens. in this work, we were surprised to identify seven tetraspanin-2 (tsp-2) homologs of the protective antigen sm-tsp-2 in schistosoma japonicum and found that the transcription profiles of sj-tsp-2 subclasses were highly variable in individual adult worms. rt-pcr revealed that sj-tsp-2 genes were transcribed in cercariae, schistosomula, adult worms, and eggs; however, ... | 2008 | 18486598 |
| determination of schistosoma japonicum circulating antigens in dilution serum by piezoelectric immunosensor and s/n enhancement. | a piezoelectric immunosensor assay was developed with immobilizing immunoglobulin g (igg) as a probe to detect schistosoma japonicum circulating antigens (sjcag). analytical strategy utilizes the polyclonal antibodies with broad-spectrum recognition to a complex target with high specificity. the immobilized antibodies were purified from immunized rabbit's sera (im-s) and infected rabbit's sera (inf-s) by s. japonicum. the detection capacities of antibodies were compared between the sera of diffe ... | 2008 | 18490150 |
| effect of floods on the transmission of schistosomiasis in the yangtze river valley, people's republic of china. | the aim of the present study was to assess the effect of floods on the transmission of schistosomiasis in the yangtze river valley, people's republic of china. retrospective analyses of malacologic, clinical and epidemiologic data, covering a 22-year period, were carried out to elucidate the dispersal patterns of intermediate host snails (oncomelania hupensis), and acute and chronic infections with schistosoma japonicum in humans in relation to floods. potential o. hupensis habitats in years wit ... | 2008 | 18499513 |
| coupling hydrologic and infectious disease models to explain regional differences in schistosomiasis transmission in southwestern china. | rainfall-runoff models have become essential tools for conceptualizing and predicting the response of hydrologic processes to changing environments, but they have rarely been applied to challenges facing health scientists. yet with their efficient parameterization and modest data requirements, they hold great promise for epidemiological application. a modeling analysis incorporating simple hydrologic constraints on transmission of the human parasite schistosoma japonicum in southwestern china wa ... | 2008 | 18505010 |
| prevalence survey of schistosomiasis in mindanao and the visayas, the philippines. | the first two phases of a national prevalence survey of schistosomiasis in the philippines were completed in mindanao in 2005 and the visayas in the first quarter of 2007. the design was a stratified two-step systematic cluster sampling, with two kato-katz thick smears examined from each participant. in mindanao, a total of 22 provinces spread in six regions were covered by the survey with five barangays (equivalent to a village) per province for a total of 110 barangays. the response rate was 7 ... | 2008 | 18508406 |
| the synergistic effect of concomitant schistosomiasis, hookworm, and trichuris infections on children's anemia burden. | to estimate the degree of synergism between helminth species in their combined effects on anemia. | 2008 | 18523547 |
| schistosomiasis in indonesia: past and present. | schistosomiasis is endemic in indonesia in two isolated areas, lindu valley and napu valley, both located in the province of central sulawesi. in 1940, a prevalence survey was initiated in lake lindu, which indicated a schistosoma japonicum infection prevalence of 56% among the population of anca, tomado and langko villages. another survey was conducted in 1973 in napu valley and very high infection prevalences of up to 72% were found among the population in winowanga village. since then, compre ... | 2008 | 18534900 |
| current developments on schistosoma proteomics. | several papers on schistosoma mansoni and schistosomajaponicum proteomes have been published worldwide in the past few years, representing an emerging field of study. knowledge of the schistosome proteome may greatly enhance our understanding of schistosome physiological processes at the molecular level, and may provide new models for development of vaccines or drugs. despite the importance of this approach, schistosome proteomic research in brazil is still incipient. here we review the developm ... | 2008 | 18539255 |
| artemether treatment of prepatent schistosoma japonicum induces resistance to reinfection in association with reduced pathology. | artemether (art) is a well-described antimalarial with efficacy against juvenile schistosomes, with 7-day-old schistosomula being particularly susceptible. both art-affected worms and parasites developing from irradiated cercariae die at similar times after infection. our aim was to determine if art treatment of prepatent schistosomiasis japonica may result in the generation of a protective immune response. female cba mice were treated with a single dose of art at defined time points after percu ... | 2008 | 18541772 |
| bayesian spatio-temporal modeling of schistosoma japonicum prevalence data in the absence of a diagnostic 'gold' standard. | spatial modeling is increasingly utilized to elucidate relationships between demographic, environmental, and socioeconomic factors, and infectious disease prevalence data. however, there is a paucity of studies focusing on spatio-temporal modeling that take into account the uncertainty of diagnostic techniques. | 2008 | 18545696 |
| a chronological research on parasitic endemic disease, "katayama-disease" (schistosomiasis japonica) in hiroshima prefecture in the 20th century (the 3rd communication on the historical research on schistosomiasis japonica in hiroshima prefecture). | a chronological research on the parasitic endemic disease, "katayama disease" in hiroshima prefecture was carried out. the present study was concerned mainly with the documents in the 20th century. it was confirmed that the development of excellent chemotherapeutic agents were not pivotal as a result. the eradication of the vector snails was clearly shown to be responsible for that of the infectious disease. | 2007 | 18548885 |
| enhancement by il-18 of the protective effect of a schistosoma japonicum 26kda gst plasmid dna vaccine in mice. | two recombinant plasmids pvax/sj26gst and pvax/mil-18 containing schistosoma japonicum 26kda gst and murine il-18 were evaluated for their ability to protect mice against s. japonicum challenge. mice were given 2 intramuscular immunizations 3 weeks apart, and challenged with s. japonicum cercariae 4 weeks later. adult worm and egg burdens were determined 48 days post-challenge. all animals vaccinated with pvax/sj26gst alone or with pvax/mil-18 developed specific anti-swap (soluble worm antigen p ... | 2008 | 18562051 |