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specific lung mucosal and systemic immune responses after oral immunization of mice with salmonella typhimurium aroa, salmonella typhi ty21a, and invasive escherichia coli expressing recombinant pertussis toxin s1 subunit.pertussis toxin (pt) is considered an essential protective component for incorporation into new generation vaccines against bordetella pertussis, the causative agent of whooping cough. traditionally, antipertussis vaccination has employed an intramuscular route. an alternative to this approach is to stimulate mucosal and systemic immune responses by oral immunization with live vaccine carrier strains of salmonella spp. or escherichia coli. recombinant s1 subunit of pertussis toxin was expressed ...19921398937
vacb, a novel chromosomal gene required for expression of virulence genes on the large plasmid of shigella flexneri.shigellae, the causative agents of bacillary dysentery, are capable of adhering to and invading epithelial cells and spreading into adjacent cells. a chromosomal mutant of shigella flexneri 2a ysh6000 with reduced invasive capacity was isolated by tn5 insertion mutagenesis. the linkage of the mutant phenotype to the tn5 insertion was determined by p1 phage transduction. the site of the tn5 insertion was assigned to a noti chromosomal restriction map, confirming that the virulence-associated locu ...19921400189
molecular analysis of the glpfkx regions of escherichia coli and shigella flexneri.we have identified a new gene, glpx, belonging to the glp regulon of escherichia coli, located directly downstream of the glpk gene. the transcription of glpx is inducible with glycerol and sn-glycerol-3-phosphate and is constitutive in a glpr mutant. glpx is the third gene in the glpfkx operon. the function of glpx remains unknown. glpx has an apparent molecular weight of 40,000 on sodium dodecyl sulfate-polyacrylamide gels. in addition to determining the e. coli glpx sequence, we also sequence ...19921400248
functional roles assigned to the periplasmic, linker, and receiver domains of the agrobacterium tumefaciens vira protein.vira and virg activate the agrobacterium tumefaciens vir regulon in response to phenolic compounds, monosaccharides, and acidity released from plant wound sites. vira contains an amino-terminal periplasmic domain and three cytoplasmic domains: a linker, a protein kinase, and a phosphoryl receiver. we constructed internal deletions of vira that truncate one or more domains and tested the ability of the resulting proteins to mediate environmentally responsive vir gene activation in vivo. the perip ...19921400253
altered-function mutations of the transcriptional regulatory gene virg of agrobacterium tumefaciens.three point mutations were isolated in the agrobacterium tumefaciens virg gene by screening for vir gene expression in the absence of added phenolic inducing compounds. all three mutations were localized in the predicted amino-terminal phosphoryl receiver domain of the protein. one mutant (n54d) bypasses the requirement for vira and phenolic inducers both for transcriptional activation of all tested vir promoters and for plant tumorigenesis. this mutant also activates vir gene expression efficie ...19921400254
primary nerve repair in the upper limb. our preferred methods: theory and practical applications.after years of controversy, it is now generally agreed that primary nerve repair by end-to-end coaptation, whenever feasible, yields better results than secondary procedures. we reviewed the theoretic basis of current methods of repair and described our preferred techniques and indications.20051400607
ingrown toenails: an evaluation of elevating the nail bed-periosteal flap.many procedures to correct ingrown toenails are available; however, they cannot avoid recurrence and nail deformities. we have performed a procedure which corrects ingrown toenail without reducing the width of the nail plate by elevating the nail bed-periosteal flap. sixty patients were observed for longer than six months and exhibited excellent results. only two cases of incurvated nails (2) required re-operation.20061401497
block of voltage-gated potassium currents by anticonvulsant u-54494a in mouse neuroblastoma cells.u-54494a [(+-)-cis-3,4-dichloro-n-methyl-n-[2-(1- pyrrolidinyl)-cyclohexyl]-benzamide], an anticonvulsant and na channel blocker, was examined for its interaction with delayed rectifier k channels in mouse neuroblastoma cells (nie-115) using whole-cell and inside-out configurations of the patch clamp techniques. u-54494a at anticonvulsant doses reversibly blocked the tetraethylammonium-sensitive outward k current in a time-dependent manner without affecting the current-voltage relationship. anal ...19921403785
temperature regulation of shigella virulence: identification of the repressor gene virr, an analogue of hns, and partial complementation by tyrosyl transfer rna (trna1(tyr)).virr is the central regulatory locus required for coordinate temperature-regulated virulence gene expression in the human enteric pathogens of shigella species. detailed characterization of virr+ clones revealed that virr consisted of a 411 bp open reading frame (orf) that mapped to a chromosomally located 1.8kb ecori-acci dna fragment from shigella flexneri. insertional inactivation of the virr orf at a unique hpai restriction site resulted in a loss of virr+ activity. the virr orf nucleotide s ...19921406252
identification and characterization of virk, a virulence-associated large plasmid gene essential for intercellular spreading of shigella flexneri.seven virulence loci have been identified by tn5 insertion mutagenesis on the large 230 kb plasmid (pmysh6000) of shigella flexneri 2a. in this study, we used tn10 insertion mutagenesis and identified a novel virulence locus on pmysh6000 responsible for bacterial spread. characterization of the invading bacteria of the tn10 insertion mutants in the epithelial cells revealed that the bacteria were capable of at least some intracellular spreading but not intercellular spreading. immunoblot analysi ...19921406277
preliminary observations concerning treatment of visual discomfort and associated perceptual distortion.a system for therapeutic precision tinting is described. some individuals who are subject to perceptual distortion of text no longer perceive the distortion when the text has a particular colour. a simple colorimeter enables an observer to illuminate text with light of a particular chromaticity, varying first cie 1976 hue angle (huv), then saturation (suv), and then luminance, to obtain a setting that maximizes visual comfort and reduces any perceptual distortion. the colorimeter setting is then ...19721408185
pain on intravenous injection. 19681409410
c-receptor ligand blocks pulmonary clearance of atrial natriuretic peptide in isolated rat lungs.pulmonary clearance of atrial natriuretic peptide (anp) was measured by indicator dilution technique in isolated perfused rat lungs with and without anp clearance receptor (c-receptor) blockade. approximately 50% of a bolus injection of 125i-anp was removed during a single pass through the lungs compared with the intravascular marker 14c-dextran. pulmonary clearance of 125i-anp was suppressed in a dose-dependent fashion by unlabeled anp. c-receptor blockade suppressed pulmonary clearance of 125i ...19921409730
induction of pancreatic acinar pathology via inhalation of nicotine.this study was conducted to determine the effects of nicotine inhalation on the onset, progression, and sequential development of pancreatic lesions. male sprague-dawley rats in groups of five were exposed to saline or nicotine aerosol twice daily for 15, 30, 45, and 60 min for 21 days. after sacrifice, blood samples were analyzed for plasma levels of nicotine, glucose, gastrin, and cholecystokinin. pancreatic tissues were examined for pathological lesions. while there were no significant differ ...19921409731
[the mechanism of the pathogenetic action of mud in treating the irritable bowel syndrome]. 20061413586
miliary tuberculosis involving the brain: mr findings. 20041414778
[competence levels]. 20061418631
[rapid detection of neurologic morbidity in full term newborn infants].the purpose of this study is to evaluate the efficacy of the simplified method to detect neurological morbidity in newborn and to estimate the predictive parameter, i.e. sensitivity, specificity and predictive value. the sample consisted of 100 newborn infants from the maternity clinic of hospital regional docente trujillo-perú, during the period from march to may 1990. all infants were born full-term, of normal delivery and apgar score of greater than or equal to 7 at one minute, and no congeni ...20061418650
regulation of erythropoiesis in the newborn: a complex system. 20061418910
the relation of communication and language development to mental retardation. 20061418929
the correlation of middle ear aeration with mastoid pneumatization. the mastoid as a pressure buffer.atelectatic ears, which by definition are poorly aerated, are also usually associated with poor mastoid pneumatization. on the other hand, otosclerotic patients, whose middle ears are usually exceptionally well aerated, also have excellent mastoid pneumatization. three unusual cases are presented, in which partial atelectasis developed in stapedectomized patients. in each case the mastoid was later found to be nonpneumatized, and further analysis revealed that their stapes fixation had in effect ...20061418937
elimination kinetics of volatile organics in humans using breath measurements.during the past decade significant strides have been made toward understanding the sources and factors which lead to volatile organic chemical (voc) exposure in the general population. less is known, however, about the impact of low-level environmental exposure on human health. investigations are underway in a number of laboratories in an effort to determine the uptake, distribution, metabolism, and elimination kinetics for vocs in humans. we examined the elimination kinetics for the third phase ...19921422163
epididymitis in children and adolescents on clean intermittent catheterisation.the records of 119 children and adolescents on clean intermittent catheterisation (cic) were reviewed to assess the incidence and predisposing factors, if any, of epididymo-orchitis. cic had been performed for a total of 448 patient years with a mean of 4.5 years (range 3 months to 19 years). of the evaluable 99 patients, 10 suffered attacks of epididymo-orchitis after commencing cic. there was an equal incidence on both sides. three patients had recurrent attacks. epididymitis occurred at the r ...20061425846
a study of the 14 kda phospholipase a2 from rat liver using a continuous fluorescent displacement assay for the enzyme. 19921426571
[intravascular autorosette-formation in hemolytic anemia].it has been established that monocyte and neutrophil autorosette-formation resultant in exocytic lysis of erythrocytes--constituents of autorosettes--takes a low-intensive course in the blood of children with chronic non-hematologic diseases. this process is significantly activated in hereditary microspherocytic and elliptocytic anemias. it has been suggested that autorosette-formation culminating in exocytic lysis of erythrocytes is a mechanism of eliminating pathological, damaged and old eryth ...19921426915
purification, partial characterization, and heterologous radioimmunoassay of growth hormone (cgh) in red deer.red deer growth hormone (cgh; 3.3 mg) was purified from an aqueous extract of seven pituitary glands (4.01 g wet weight) by preparative gel filtration on sephadex g-100, gel filtration on sephadex g-100 sf, and anion exchange chromatography on deae-sepharose cl-6b. purified cgh gave a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with a molecular weight under reducing conditions of 20,000 da and gave a single peak on reverse-phase high-performance liquid chromatography ...19921426952
lorenz-mie light scattering in cellular biology.the lorenz-mie light scattering is discussed as a tool allowing living cell characterization. the scattered light carries information about the size, shape, internal structure and refractive index of the cell. the advantages of light scattering methods consist in high speed, nondestructive, sensitive and relatively easy measurements. light scattering methods are compatible with other methods. in light scattering in both static and flow systems. for sphere-like cells reliable size and refractive ...20061426967
candida albicans--saprophyte or pathogen?skin scrapings taken from toe spaces of 200 healthy volunteers and from toe webs and groins of 150 pediatric patients were cultured for candida albicans using the serum germ-tube test. the results showed that candida albicans can be isolated in about 15% of normal toe spaces and 14% of children with normal groins. although candida albicans can be found in various grades of athlete's foot and also in some abnormal groins, we believe that it is not necessarily responsible for these conditions and ...19921428429
[combined resections in the surgery of lung cancer].operations were performed on 3,313 patients for lung carcinoma in the department of surgery of the lungs and mediastinum of the national research center of surgery, ams ussr, from 1963 till 1.01.90. among these patients 263 (7.9%) were treated by combined operations (resection of the lung together with part of the chest wall, trachea and its bifurcation, pericardium, diaphragm, superior vena cava, pulmonary artery, aorta, and atrium, and also with removal of metastatic lymph nodes of the contral ...19881434353
[bacteriological examination of paper money].bacteriological survey of one hundred twenty currency notes was done. aerobic spore-forming bacilli (91%), staphylococcus epidermidis (63.3%), staphylococcus aureus (4.2%), enterococcus (24.1%), alpha-hem. streptococcus (4.1%), streptococcus pneumoniae (1.7%), corynebacterium (7.5%), lactobacilli (10.8%), klebsiella pneumoniae (31.7%), enterobacter (19.2%), e. coli (17.5%), proteus (1.7%), pseudomonas aeruginosa (0.8%), shigella flexneri (0.8%) were isolated from paper money samples. currency no ...19921435365
thrombocytopenia and the neglected megakaryocyte. 20061435936
genotypic and phenotypic characterization of an arod deletion-attenuated escherichia coli k12-shigella flexneri hybrid vaccine expressing s. flexneri 2a somatic antigen.the construction and characterization of ecsf2a-2, an arod-deleted escherichia coli-shigella hybrid vaccine carrying chromosomal and plasmid genes from shigella flexneri and expressing s. flexneri 2a somatic antigen in association with e. coli k12 core are described. expression of hybrid lipopolysaccharide and deletion of arod resulted in the attenuation of phenotypic characteristics associated with pathogenicity. the addition of an arod deletion results in a requirement for an aromatic precurso ...19921441732
[lipopolysaccharide changes in smooth-type avirulent shigella in comparison with initial virulent strains].the comparative study of the lipopolysaccharides (lps) of virulent and avirulent strains of s. sonnei, phase i (smooth colonies), has been made. electrophoresis of lps and subsequent densitometry of electrophoregrams have revealed the increase of the fraction of long 0-chains with a considerable number of recurring elements in 2 out of 3 lps preparations obtained from avirulent shigellae. in mice immunized with these lps preparations a considerably greater number of antibody-producing cells can ...19921441805
gametophytic self-incompatibility: biochemical, molecular genetic, and evolutionary aspects. 20061446981
"raynaud's disease" in migraineurs: one entity or two?two cases are reported of patients who were diagnosed as having both migraine and raynaud's disease. because they were able to achieve effective control of both headaches and extremity temperature after undergoing a comprehensive approach--utilizing medication, biofeedback, and psychotherapy--it is suggested that both conditions are manifestations of the same disorder. it is recommended that a first step in approaching concurrent diagnoses of this type involve combined treatment as is often used ...20061446993
empty wenckebach pauses. 20061447006
the resurgence of free radicals. 20061447450
concentration of milk secretory immunoglobulin a against shigella virulence plasmid-associated antigens as a predictor of symptom status in shigella-infected breast-fed infants.we conducted a prospective, community-based study of healthy breast-fed mexican infants to determine the protective effects of anti-shigella secretory iga antibodies in milk. milk samples were collected monthly, and stool culture specimens were obtained weekly and at the time of episodes of diarrhea. nineteen breast-fed infants were found to have shigella flexneri, shigella boydii, or shigella sonnei in stool samples. ages of the 10 infants with symptomatic infection and the nine with asymptomat ...19921447644
[characteristics of the dynamics of hematopoietic precursor cells cloned in diffusion chambers and recorded in experiments on mice and dogs irradiated in median lethal doses].in experiments with mice and dogs irradiated with ld50, it was shown the postirradiation depopulation of haemopoietic polypotent (cfus) cell-precursors in mouse bone marrow was more pronounced than that of granulocytic and macrophagal cells (cfudc). the rate of repopulation of cfus during the first week was higher than that of cfudc (t1/2 was 2.5 and 8.8 days respectively). in dogs, one could notice a partial change in the colony formation, a prolonged plateau period in the postirradiation cfudc ...19921448562
diaphragmatic movement using ultrasound during spontaneous and mechanical ventilation: effect of tidal volume.using ultrasound (us) the effect of various tidal volumes on the movement of ventral, dome and dorsal parts of the right hemidiaphragm was studied, both during spontaneous and mechanical ventilation. six healthy non-medicated volunteers who were in the supine position breathed spontaneously shallowly (tidal volume (vt) being 400 ml) (sb), and deeply (vt 1000 ml) (sb-deep). in addition, they were mechanically ventilated with intermittent positive pressure ventilation at three different vt's: 500 ...20061449048
haloperidol does not alter expression of ethanol-induced conditioned place preference.a recent experiment (risinger et al., psychopharmacology, 107 (1992) 453-456) has shown that haloperidol does not prevent acquisition of ethanol-induced conditioned place preference, suggesting that dopaminergic mechanisms do not mediate the primary rewarding properties of ethanol. the present experiment examined whether haloperidol would prevent the expression of conditioned reward to ethanol-paired stimuli using the place conditioning paradigm. dba/2j mice received four pairings of a tactile s ...20061449638
[bacteremia caused by shigella flexneri]. 19921450269
shigellosis in children: a prospective hospital based study.from 1985 to 1988, fecal samples of 950 hospitalized children suffering from diarrhea or dysentery were screened for shigella species using standard methods. shigella species were isolated as sole pathogen from 192 (20.2%) cases and s. flexneri type 2 was the predominant serotype. shigella infection was prevalent throughout the year with high isolation rate during the summer and early monsoon months. shigella strains isolated during the period were resistant to most of the commonly used drugs fo ...19921452309
pathogenesis of providencia alcalifaciens-induced diarrhea.providencia alcalifaciens is a member of the family enterobacteriaceae. there are reports that p. alcalifaciens can cause diarrhea, but the mechanism(s) by which it causes diarrhea is known. we studied p. alcalifaciens isolated from a child and two adults with diarrhea for enteropathogenicity. the three isolates did not exhibit any characteristic adherence to cultured hep-2 cell monolayers, and they did not produce enterotoxins, cytotoxins, or keratoconjunctivitis in the sereny test. two isolate ...19921452332
detection of shigella dysenteriae type 1 and shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction.a combination of immunomagnetic separation (ims) and a polymerase chain reaction (pcr) procedure was used for direct isolation and identification of shigella dysenteriae type 1 and shigella flexneri from feces. immunomagnetic particles were coated with monoclonal antibody masfb, which is specific for a common epitope of the o polysaccharides of s. dysenteriae type 1 and s. flexneri. bacteria bound to the beads were boiled in water, and target dna was amplified with a primer pair specific for a g ...19921452650
[active tuberculosis in children who received inh chemoprophylaxis].twelve children who developed active tuberculosis even after receiving isoniazid (inh) chemoprophylaxis were seen at tokyo metropolitan children's hospital from 1982 through 1991. all cases received inh more than 9 mg/kg/day, except for one case in which the amount of inh administered at the referring hospital was unknown and streptomycin was administered together with inh. the age of starting inh prophylaxis ranged from 2 months to 13 years, and the age at which clinical symptoms and/or laborat ...19921453566
present status of research abroad concerning the effect of acupuncture and moxibustion on immunologic functions.japan is one of the countries that have done extensive research on the effect of acupuncture and moxibustion on the immunologic functions. with china first, japan ranks second among the countries in this study. concerning research in this field concerned the level abroad can scarcely reach that in china. scholars abroad fail to base their research on tcm theory. in addition not only the type of diseases studied, and the indexes observed are far less than those in china, but also the scope of the ...20061453763
[disorders of tubular transport of uric acid leading to hyperuricemia]. 19841454659
[the regulation of dna repair processes in mammalian cells. iii. the effect of epidermal growth factor on the postradiation recovery of the cell cycle in human a 431 cells and embryonic fibroblasts].recovery of the cell cycle in cells a 431 and in human embryo fibroblasts (efh) differs much. unlike efh, a 431 cells have: 1) synchronized exit of cells from g1 into s phase after 5 gr irradiation; 2) g2-block; 3) much less manifestation of these two phenomena in the presence of egf; 4) a lesser effectiveness of the repair of dna single-strand breaks. egf stimulation of the repair of radiation-induced dna lesions, ssb in particular, may be of great importance for the postirradiation cell cycle ...19921455555
retinal and cortical activity in human subjects during color flicker fusion.pattern electroretinograms (perg) and cortical visually evoked potentials (vep) were simultaneously recorded from 7 visually normal and 1 protanopic subjects. stimuli were color checkerboards (0.5 degrees check size), phase-reversing at 17 hz (i.e. 34 reversals/sec). using a stepwise sweep procedure, the luminance of the red (lambda peak = 550 nm) and green (lambda peak = 630 nm) checks varied in 11 steps in opposite directions from 0 to 30 cd/m2, embracing the subjective equiluminance point. fo ...19921455696
failure of rivalry at low contrast: evidence of a suprathreshold binocular summation process.presentation of different images to the two eyes normally results in a time-varying alternation between the two images (binocular rivalry). however, we find that when orthogonal gratings are viewed dichoptically at low contrast, a stable summation between the two images is perceived in the form of a dichoptic plaid. the range of perception of the dichoptic plaid depends on spatial frequency, contrast and luminance of the gratings. this phenomenon differs from the "false fusion", a fleeting summa ...19711455720
[the immunocorrective therapy of patients with acute dysentery]. 19921455821
[the excretion of shigella antigens in persons recovering from dysentery].shigella antigens can be detected in the excreta of convalescents after dysentery for a long time. most frequently these antigens occur in feces, less frequently in urine and rarely in saliva. according to indirect data, s. flexneri 1-6 antigens can be detected in excreta for a longer period after convalescence than s. sonnei antigens. when antigen indication is used for the diagnosis of dysentery and epidemiological analysis is carried out, one should bear in mind the length of the agent persis ...19921455962
[the etiological structure of shigellosis in the former ussr--an indicator of the activity of the main routes of infection transmission].the data on the etiological structure of shigella infections in the ussr in 1988-1989 are presented. the study showed the dominating role of s. flexneri with s. sonnei also retaining great importance in shigella infections. the process of the liquidation of s. dysenteriae and s. boydii infections began in some large cities. the domination of dysentery caused by s. flexneri and a high typhoid rate, particularly in central asia, were due to poor water supply of the population. the spread of dysent ...19921455964
[an increase in the immunogenicity of bacterial antigens under the influence of one of the derivatives of muramyl dipeptide].as revealed in animal experiments, glucosaminylmuramyl dipeptide (gmdp), the synthetic analog of muramyl dipeptide, when introduced intraperitoneally in a single injection or orally, exhibits adjuvant activity with respect to citrobacter 0-antigens, shigella flexneri and enhances the protective properties of dysentery and pertussis vaccines. the stimulating properties of gmdp depend on its dose, the route of its administration, the time elapsed after its administration, its ratio to the concomit ...19921455969
[the clinical assessment of the immune status of patients with acute flexner shigellosis].a total of 192 patients with flexner's dysentery 1 have been examined. as a result, sharply pronounced unbalance of cell-mediated and humoral immunity factors has developed in the acute period of dysentery, reaching its maximal manifestations in severe course of the disease. in case of a tendency to a prolonged course of dysentery at the period of convalescence, essential shifts in immunological characteristics persist and the infective agent is repeatedly isolated.19921455972
apparatus criticus: methods used to evaluate growth modification in class ii malocclusion. 20061456234
red blood cells as an antigen-delivery system.the use of adjuvants is usually required to induce strong immunological responses to protein antigens. however, in many cases these adjuvants cannot be extensively applied in human and veterinary vaccinations because of associated inflammatory reactions or granuloma formation. we show here that protein antigens (bovine serum albumin, hog liver uricase, and yeast hexokinase), coupled to autologous red blood cells by way of a biotin-avidin-biotin bridge, elicit an immunological response in mice si ...19921457052
[roentgeno-morphologic changes in the bones of battered children].the demonstrated characteristic bone lesions should raise the suspicion of "battered child syndrome". the important role of the radiologist is stressed.19791457479
caution advised when changing to new therapies. 20061458280
sister chromatid exchange (sce) frequency in lymphocytes of patients with colorectal carcinoma treated with razoxane.the increased risk of neoplasia following cytotoxic therapy for both malignant and nonmalignant disease is well known. sister chromatid exchange (sce) frequencies in patients receiving such chemotherapy are often elevated, and the persistence of high levels after treatment may provide an indicator for susceptibility to secondary neoplasia. the cytostatic drug razoxane has been used for the treatment of psoriasis, acute myeloid leukemia (aml), and colorectal carcinoma. prolonged use of this drug, ...20061458513
loose stools in the early neonatal period.during a two-year period, the various factors associated with loose stools in the early neonatal period were studied among hospital born babies. low birth weight babies had a lower incidence of non-infective loose stools when compared to neonates with a birth weight of more than 2500 g (p < 0.001). newborns delivered by cesarean section (p < 0.001) and those born to women with more than two children (p < 0.02) had a greater frequency of loose stools. initiation of supplementary feeding and admin ...19921459697
aminoglycoside nephrotoxicity in clinical practice. 20061459713
cancer-cell traffic in the liver. i. growth kinetics of cancer cells after portal-vein delivery.following the intrasplenic injection of b16f10 melanoma cells into mice, at first single cells, and later multicellular tumor foci were observed at different times in the liver. cell numbers and tumor volumes were determined over the next 12 days, by confocal microscopy of thick liver sections. fifteen minutes after injection, approximately 20% of the melanoma cells were identified in the liver microvasculature; after 48 hr, only 0.68% of these retained morphologic integrity; by 5 days only 0.13 ...20061459739
determining the variability of performance on wingate anaerobic tests in children aged 6-12 years.in two parts, this study investigated variability of performance in wingate anaerobic tests (wants) of normal, healthy, male children aged six, eight, ten and twelve years. the purpose of the first part of the study was to investigate the mean coefficient of variation (c.v. = [s.d./x].100) of children who performed four wants over a period of four weeks. the results revealed age-related differences in the performance data of peak power (pp) and mean power (mp) but no differences between age grou ...20061459745
response of beta-endorphin and estradiol to resistance exercise in females during energy balance and energy restriction.the acute effect of weightlifting on beta-endorphin and estradiol was studied in experienced female recreational weightlifters. five eumenorrheic females completed two months of testing, each with a different sequence of testing conditions (seq1 and seq2). in seq1, a week of weight maintenance diet and prescribed exercise (3 d.wk-1, 3 sets, approximately 85% 1 rm, 10-12 reps, eight lifts) beginning on d 11 of their menstrual cycle was followed by measurement of hormone response to a weightliftin ...20061459750
[diagnosis of acute diarrheal infections]. 19921460834
characteristics of polymorphism at a vntr locus 3' to the apolipoprotein b gene in five human populations.we have analyzed the allele frequency distribution at the hypervariable locus 3' to the apolipoprotein b gene (apob 3' vntr) in five well-defined human populations (kacharis of northeast india, new guinea highlanders of papua new guinea, dogrib indians of canada, pehuenche indians of chile, and a relatively homogeneous caucasian population of northern german extraction) by using the pcr technique. a total of 12 segregating alleles were detected in the pooled sample of 319 individuals. a fairly c ...19921463014
the gene for familial mediterranean fever in both armenians and non-ashkenazi jews is linked to the alpha-globin complex on 16p: evidence for locus homogeneity.familial mediterranean fever (fmf) is a recurrent inflammatory disorder characterized by short episodes of fever, peritonitis, pleuritis, and arthritis. while fmf has been shown to be inherited in an autosomal recessive fashion in both non-ashkenazi jews and armenian families, clinical differences have raised the possibility of genetic heterogeneity. as its pathogenesis is unknown, mapping of the gene for fmf may provide the first objective method for early and accurate diagnosis of this disease ...19921463015
comparative effects of thyroxine and/or retinoic acid treatment in vivo on growth hormone synthesis and release by pituitaries from thyroidectomized rats.thyroid hormones and retinoic acid (ra) coregulate growth hormone (gh) synthesis and release from cultured pituitary tumor cells by interacting with nuclear receptors that activate gh gene transcription. whether these two compounds share overlapping gh regulatory activities in vivo is unclear. therefore we compared the effects of in vivo replacement therapy with thyroxine (t4) and/or retinoic acid (ra) on gh synthesis and release in pituitaries from hypothyroid rats. three weeks after thyroidect ...19921464411
the effect of insulin treatment on hba1c, body weight and lipids in type 2 diabetic patients with secondary-failure to sulfonylureas. a five year follow-up study.no conclusive data are available about the long-term effect of insulin treatment in type 2 diabetic patients failing to maximal doses of sulfonylureas and caloric restriction. in total 160 non-obese type 2 diabetic patients with secondary failure were substituted with insulin using a diabetic teaching and care program. from these 160 patients 40 died within the observation period of five years; 102 patients had a complete five year follow-up, whereas the remaining 18 patients did not come to reg ...20061464414
effects of 5-fluorouracil on hematopoietic stem cells in normal and irradiated mice.the effects of 5-fluorouracil (5-fu) on hematopoietic stem cells (cfu-s) and nucleated cells in mouse femur and spleen were studied in normal and x-irradiated mice (ddy-slc male, 8-10 weeks old). changes in the number of circulating blood cells also were investigated in mice treated with 5-fu. a single dose of 5-fu (150 mg/kg) was injected i.p. the femoral cfu-s decreased after 5-fu treatment up to day 3 when it reached 2% of the control value. the cells then increased, reaching a maximum about ...19921464854
[the theoretical and practical interpretations of the basic manifestations of the epidemic process in shigellosis]. 19921466177
expression of active octameric chicken cardiac mitochondrial creatine kinase in escherichia coli.sarcomeric mitochondrial creatine kinase (mib-ck) of chicken was expressed in escherichia coli as a soluble enzyme by using an inducible phage-t7 promoter. up to one third of the protein in e. coli extracts consisted of soluble recombinant mib-ck in an enzymically active form. approx. 20 mg of nearly-homogenous mib-ck was isolated in a two-step isolation procedure starting with 1 litre of isopropyl beta-d-thiogalactopyranoside-induced e. coli culture, whereas previous attempts to express other c ...19921471992
solitary cervical lymphoma presenting as a neurofibroma.a patient is described who presented with cervical cord compression. the imaging and operative findings were typical of a neurofibroma of the right third cervical root. however, histological studies confirmed that the tumour was a b-cell lymphoma. isolated spinal lymphomas can therefore occur and may present as nerve sheath tumours.20061472324
determinants of pathogenicity in xanthomonas campestris pv. vesicatoria are related to proteins involved in secretion in bacterial pathogens of animals.one of the model systems investigated for studying plant bacterial pathogenesis is xanthomonas campestris pv vesicatoria, the causal agent of bacterial spot disease of pepper and tomato. genes necessary for both basic pathogenicity and the induction of the hypersensitive response in resistant plants (hrp genes) were previously isolated from x. c. pv. vesicatoria and characterized genetically. as a first step toward functional analysis, part of the hrp gene cluster, making up several loci, was se ...19921472717
in memoriam: peter mitchell, 1920-1992. 20061474903
antibody response to oral streptococci in behçet's disease.the serum antibody titers against oral streptococci were studied by enzyme-linked immunosorbent assay (elisa) both in patients with behçet's disease (bd) and control groups. the patients with bd showed significantly higher antibody titers to s. sanguis strains 113-20, 114-23, and 118-1 which were isolated from patients with bd, in comparison with control groups. also, the reactions of high-titered sera to the crude cell wall and soluble (or membrane) fractions of the 113-20 strain were observed ...19921474932
[bacillary dysentery--1990]. 19921475396
glyceryl trinitrate for angina: tablet or spray? 20061478166
interictal hm-pao spect: a routine investigation in patients with medically intractable complex partial seizures?single photon emission computed tomography (spect) is increasingly being used as an adjunctive technique in the localisation of epileptogenic foci prior to surgery. as yet, few studies have been undertaken to establish the clinical associations of areas of reduced cerebral perfusion. sixty-three consecutive patients (15 male, 48 female; median age 30 years, range 14-57 years) with medically intractable complex partial seizures (median/month 8, range 1-36) were investigated as outpatients. all ha ...20061478200
[free radicals and diseases]. 20061479233
[the causes of cerebral complications in patients with ischemic heart disease following myocardial revascularization operations].in the period from jan. 1988 to jan. 1991 operations for the formation of an aortocoronary shunt were carried out in 437 patients at the department of combined affections of the coronary and peripheral arteries, bakulev research institute. in some cases the operation was combined with resection of a postinfarction aneurysm of the left ventricle. cerebral complications developed in the postoperative period in 28 (6.4%) patients. their analysis showed the prevalence of severe forms of consciousnes ...20061482586
16th annual symposium on computer applications in medical care. proceedings. baltimore, maryland, november 8-11, 1992. 20061482848
collaborative care documentation by exception system.development of a workstation-based documentation system supporting collaborative care and nurse charting by exception has been underway for the last two and one-half years at vanderbilt university medical center in nashville, tennessee. the pathways system has undergone two major revisions and is currently implemented on three nursing units. the system supports collaborative care by providing a mechanism for entry and printing of collaborative care pathways and collection of exception data. pape ...20061482850
rounds: a customizable help results review program for hospital staff physicians.physicians rely on the ready availability of clinical data such as laboratory results and procedure reports for the delivery of quality patient care. such data may be obtained through telephone calls, printed reports or results review programs provided by hospital information systems, but the process is often time-consuming and laborious. to facilitate rapid access to critical patient data, an online m.d. rounds report program, rounds, was developed as an adjunct to the help system at rex hospit ...20061482891
increases in knowledge and use of information technology by entering medical students at mcmaster university in successive annual surveys.to determine self-reported microcomputer and information technology competency, access, and usage by entering medical students and their perceptions of the need for training in additional applications.20061482936
the process and outcome of a collaborative effort to design and implement a clinician order processing system. 20061482980
scut: clinical data organization for physicians using pen computers.the role of computers in assisting physicians with patient care is rapidly advancing. one of the significant obstacles to efficient use of computers in patient care has been the unavailability of reasonably configured portable computers. lightweight portable computers are becoming more attractive as physician data-management devices, but still pose a significant problem with bedside use. the advent of computers designed to accept input from a pen and having no keyboard present a usable computer ...20061483012
chartline: providing bibliographic references relevant to patient charts using the umls metathesaurus knowledge sources.a successful medical informatics program helps its users to match their information needs as closely and efficiently as possible to the capabilities of the system. chartline is a computer program whose input is a free text, "natural language" patient chart in ascii format. using the umls metathesaurus knowledge sources, chartline can suggest bibliographic references relevant to the patient case described in the chart. the program does not attempt to "understand" the natural language content of t ...20061483014
watching a generation die: canadian doctors and the battlefields of world war i. 20061483240
[chemical and serological study of lipopolysaccharide isolated from shigella flexneri 88-893 possessing a new type-antigen].the o-specific polysaccharide chain which represents a new type-antigen in lipopolysaccharide (lps) of shigella flexneri 88-893 was investigated. the o-polysaccharide chain was found to be composed of repeating units comprising rhamnose, n-acetylglucosamine and glucose (3:1:2). in the passive hemolysis test, group-6 antiserum of s. flexneri exhibited a high hemolytic titer (50% hemolysis titer: 7,900) against sheep red blood cells (srbc) sensitized with intact 893 lps, but virtually no hemolytic ...19921484404
the mode of action of cfad of escherichia coli and virf of shigella flexneri and other members of the arac family of positive regulators. 19921484494
radiotherapy of malignant melanoma of the uvea with i-125 seeds.nineteen patients with malignant uveal melanomas were treated with i-125 applicators. there were 10 males and 9 females with a median age of 61 years (range 42-76). the tumour was located in the choroid in 12 eyes and in the choroid and ciliary body in 7 eyes. the size of the tumours was 7-18 mm in maximal basal diameter (median 12), 5-16 mm in minimal basal diameter (median 10), and 5.5-15 mm in thickness (median 8.5). the volume of the tumours was 123-1890 mm3 (median 540). all tumours were cl ...20061488887
academic success of integrated hearing-impaired pupils who had enrolled in the early written-oral language program.data on academic success were analyzed for 37 hearing-impaired pupils who had been trained by the written-oral language method before school age. seventy-one percent of pupils whose average hearing level was less than 89 db and 77% of pupils whose hearing was 90 to 120 db succeeded academically. degree of hearing impairment, speech intelligibility, and gender were not important to their success. linguistic competence evaluated just before enrollment in a normal educational setting at 6 years of ...20061489281
[significance of drug determination in pilocarpine sweat for detection of past drug abuse].the presence of cocaine, morphine and methadone in sweat samples obtained after stimulation of the eccrine sweat glands, from drugs users after six drugs-free days, was investigated. the stimulation of the sweat elimination was proved using pilocarpine-iontophoresis every hour for 7 hours. the drugs concentrations were determined by radioimmunoassay. consequently, the values measured represent the sum of the drug and its metabolites. measurable levels of cocaine, morphine and methadone were obta ...20061489310
[multifactorial studies of 154 fatalities of psychotropic drug poisoning].the documents of 154 drug victims dissected in the centre of legal medicine of the university of frankfurt am main in 1987 and 1988 were examined by computer. the average age i.e. was 26 years, the share of women 23 per cent. in 47 per cent the main cause of death was an overdoses of heroin and in 48 per cent heroin was the first drug that was taken. 58 per cent of the drug victims came from totally intact families.20061489313
[rectal temperature-time of death nomogram: dependence of corrective factors of body weight in significant thermal insulating conditions].98 test coolings were made under various cooling conditions (moving air, two types of both clothing and covering) on dummies of real masses of 1, 3.3, 9.9, 24.5 and 33.4 kg resp. which cool under standard conditions (unclothed, uncovered, still air) like human bodies of 14, 33, 41, 83 and 104 kg resp. the results provide evidence on a nonlinear dependence of corrective factors of body weight upon the body weight. the dynamics of the dependence increases with the thickness of thermic isolation. t ...19921489321
rotator cuff disease: assessment with mr arthrography versus standard mr imaging in 36 patients with arthroscopic confirmation.standard proton-density- and t2-weighted magnetic resonance (mr) imaging and mr arthrography were used to depict rotator cuff disease in 36 shoulders in 36 patients; the findings were compared with arthroscopic findings in every patient. in 19 rotator cuffs normal at arthroscopy, mr arthrography revealed no tear in 16 patients, a partial tear in one patient, and a full-thickness tear in two patients. standard proton-density- and t2-weighted images were normal in 15 of these patients and revealed ...20061489363
asbestos: the turbulent interface between science and policy. 20061489439
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