Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| the roles of e6-ap and mdm2 in p53 regulation in human papillomavirus-positive cervical cancer cells. | the p53 tumor suppressor is regulated by the mdm2 oncoprotein through a negative feedback mechanism. mdm2 promotes the ubiquitination and proteasome-dependent degradation of p53, possibly by acting as a ubiquitin ligase. in cervical cancer cells containing high-risk human papillomaviruses (hpv), p53 is also targeted for degradation by the hpv e6 oncoprotein in combination with the cellular e6-ap ubiquitin ligase. in this report, we describe the identification of efficient antisense oligonucleoti ... | 2000 | 10726657 |
| the amyloid beta peptide abeta (25-35) induces apoptosis independent of p53. | apoptosis of neuronal cells apparently plays a role in alzheimer's disease (ad). the amyloid beta (abeta) peptide derived from beta-amyloid precursor protein is found in ad brain in vivo and can induce apoptosis in vitro. while p53 accumulates in cells of ad brain, it is not known if p53 plays an active role in abeta-induced apoptosis. we show here that inactivation of p53 in two experimental cell lines, either by expression of the papillomavirus e6 protein or by a shift to restrictive temperatu ... | 2000 | 10734238 |
| development and assessment of a general theory of cervical carcinogenesis utilizing a severe combined immunodeficiency murine-human xenograft model. | currently, we lack a theoretical explanation for why squamous cell cervical cancer develops predominantly in specific sites (i.e., along the squamocolumnar junction). we therefore implanted human cervical tissues containing the transformation zone in severe combined immunodeficiency (scid) mice and studied morphology, steroid effects, gene expression, and human papillomavirus (hpv) factors. | 2000 | 10739703 |
| 5-fluorouracil-induced apoptosis in cultured oral cancer cells. | chemotherapy is commonly used to treat advanced oral squamous cell carcinoma (scc) and is known to kill cancer cells through apoptosis. our hypothesis states that 5-fluorouracil (5fu) also kills cultured oral epithelial cells through programmed cell death or apoptosis. cultured oral cancer cells were exposed to an optimum dose of 20 mg/ml of 5fu. cells were analyzed for changes in cell cycle distribution and induction of cell death including apoptosis. normal control, human papilloma virus-immor ... | 2000 | 10745178 |
| epstein-barr virus (ebv) genes expression in cervical intraepithelial neoplasia and invasive cervical cancer: a comparative study with human papillomavirus (hpv) infection. | to elucidate a causative role of epstein-barr virus (ebv) for cervical cancer, presence and expression of ebv genes were examined in 31 cervical carcinomas (icc), 23 cervical intraepithelial neoplasias (cin), and 35 normal cervices (ncx). in reverse transcription polymerase chain reaction (rt-pcr) analysis, eber-1 mrnawas expressed in 74% (23/31) of icc, 83% (19/23) of cin, 37% (13/35) of ncx. lmp-1 was expressed in 52% (16/31) of icc, 70% (16/23) of cin, and 23% (8/35) of ncx, and ebna-2 was ex ... | 2000 | 10746674 |
| role of routine human papillomavirus subtyping in cervical screening. | human papillomavirus testing has been evaluated as an adjunct to cervical cytologic screening. there is a well-established association between high-risk malignant subtypes of human papillomavirus infection and the development of neoplastic change. although human papillomavirus infection is a necessary condition for malignant transformation, it is not a sufficient condition. multiple cofactors are necessary for progression to dysplasia and then to invasive cervical cancer. because of the multifac ... | 2000 | 10752510 |
| p53 codon 72 polymorphism and susceptibility to skin cancer after renal transplantation. | infection with human papillomavirus (hpv) is an important risk factor for the development of skin cancer after renal transplantation. it has recently been suggested that degradation of the tumor suppressor gene p53 is an important mechanism for human papillomavirus-induced carcinogenesis. a common genomic polymorphism occurs at codon 72 of the p53 gene, and in vitro the codon 72arg variant appears to be particularly susceptible to degradation. | 2000 | 10755564 |
| advances in cervical screening technology. | the pap smear unquestionably is a successful screening test for cervical cancer. however, recent advances in technology have raised questions regarding whether the conventional pap smear is still the standard of care. this article relates issues of screening and cost-effectiveness to the state of the art in thin layer preparations, cytology automation, human papillomavirus screening, human papillomavirus vaccines, and other cervical screening adjuncts. perhaps nowhere in medicine is clinical dec ... | 2000 | 10757338 |
| human papilloma virus in melanoma biopsy specimens and its relation to melanoma progression. | to evaluate melanoma biopsy specimens for human papilloma virus (hpv) and determine the relation between the presence of hpv, in vitro growth, and clinical progression of melanoma in the patients from whom the biopsy specimens were derived. | 2000 | 10767787 |
| [effects of human papilloma virus types on course of juvenile respiratory papillomatosis and effectiveness of interferon therapy in children]. | identification of the type of human papilloma virus (hpv) was done by means of polymerase chain reaction in 40 children with juvenile respiratory papillomatosis (jrp). adjuvant to surgery interferon treatment was carried out in 18 patients (study group) with severe jrp. the rest 22 patients (control group) with less severe papillomatosis received only surgical treatment. the majority of the patients from both the groups had the virus type 6/11 (72.2 and 77.3% for the study and control groups, re ... | 2000 | 10771610 |
| human papillomavirus type 16 e7 oncoprotein represses transcription of human fibronectin. | the e7 oncoprotein encoded by human papillomavirus (hpv) type 16 repressed the transcription of fibronectin, a key component of the extracellular matrix. this repression, detected in several hpv-positive nontumorigenic and tumorigenic cell lines, was abolished when the cys-x-x-cys repeats in e7 were disrupted. | 2000 | 10775635 |
| human papillomaviruses and vulvar vestibulitis. | to assess the relationship between human papillomavirus (hpv) infection and vulvar vestibulitis syndrome. | 2000 | 10775729 |
| wild-type p53 transactivates the killer/dr5 gene through an intronic sequence-specific dna-binding site. | killer/dr5, a tumor necrosis factor-related apoptosis-inducing ligand (trail) death receptor gene, has been shown to be induced by dna damaging agents and radiation in a p53-dependent manner. although trail is a potential therapeutic agent for cancer, the induction mechanism of its receptors is poorly understood. here we show the identification of three p53 dna-binding sites in the killer/dr5 genomic locus located upstream (bs1; -0.82 kb) of the atg site, within intron 1 (bs2; +0.25 kb downstrea ... | 2000 | 10777207 |
| mutagenesis of the prb pocket reveals that cell cycle arrest functions are separable from binding to viral oncoproteins. | the pocket domain of prb is required for prb to arrest the cell cycle. this domain was originally defined as the region of the protein that is necessary and sufficient for prb's interaction with adenovirus e1a and simian virus s40 large t antigen. these oncoproteins, and other prb-binding proteins that are encoded by a variety of plant and animal viruses, use a conserved lxcxe motif to interact with prb. similar sequences have been identified in multiple cellular prb-binding proteins, suggesting ... | 2000 | 10779361 |
| characterization of pdz-binding kinase, a mitotic kinase. | hdlg, the human homologue of the drosophila discs-large (dlg) tumor suppressor protein, is known to interact with the tumor suppressor protein apc and the human papillomavirus e6 transforming protein. in a two-hybrid screen, we identified a 322-aa serine/threonine kinase that binds to the pdz2 domain of hdlg. the mrna for this pdz-binding kinase, or pbk, is most abundant in placenta and absent from adult brain tissue. the protein sequence of pbk has all the characteristic protein kinase subdomai ... | 2000 | 10779557 |
| a simplified and reliable hpv testing of archival papanicolaou-stained cervical smears: application to cervical smears from cancer patients starting with cytologically normal smears. | the efficacy of four methods to recover dna from papanicolaou (pap)-stained archival cervical smears for optimal detection of human papillomavirus (hpv) dna by gp5+/biogp6+ polymerase chain reaction (pcr) was investigated. two of the methods were based on proteinase k treatment and two based on treatment with guanidinium thiocyanate (gtc). the quality of the dna as measured by pcr assays amplifying different sizes of the beta-globin gene appeared to be superior for the gtc-based assays. using co ... | 2000 | 10780521 |
| widespread elevated expression of the human papilloma virus (hpv)-activating cellular transcription factor brn-3a in the cervix of women with cin3 (cervical intraepithelial neoplasia stage 3). | 2000 | 10781392 | |
| prognostic implication of human papillomavirus infection in squamous cell carcinoma of the lung. | on the subtropical island of okinawa, squamous cell carcinoma (scc), particularly the well-differentiated form, is the most frequent type of lung cancer, while this form is relatively rare on the japanese mainland and in other countries. furthermore, in okinawa, in 1993, 80% of scc cases of the lung were found to be infected with human papillomavirus (hpv). we studied the prognosis of scc of the lung with hpv infection (n = 25) and compared it with non-hpv-infected scc (n = 16). using the kaplan ... | 2000 | 10782464 |
| cancer in husbands of cervical cancer patients. | we used the swedish family-cancer database to analyze the spectrum of cancers diagnosed in husbands of women with in situ or invasive cervical cancer, and we compared these to second carcinogenic events in women presenting with these cancers. our hypothesis was increased cancer susceptibility from human papilloma virus (hpv). when the wives had in situ or invasive cervical cancer, the standard incidence ratios (sirs) for anal cancer in husbands were 1.75 (95% ci = 1.05-2.62) and 1.92 (95% ci = 0 ... | 2000 | 10784257 |
| retinoic acid resistance at late stages of human papillomavirus type 16-mediated transformation of human keratinocytes arises despite intact retinoid signaling and is due to a loss of sensitivity to transforming growth factor-beta. | in our in vitro model of human cell carcinogenesis, normal human foreskin keratinocytes (hkc) transfected with human papillomavirus type 16 dna (hkc/hpv16) progress toward malignancy through several phenotypically defined and reproducible "steps" that include immortalization, growth factor independence (hkc/gfi), differentiation resistance (hkc/dr), and ultimately malignant conversion. while hkc/hpv16 are very sensitive to growth inhibition by all-trans-retinoic acid (ra) at early passages, they ... | 2000 | 10792999 |
| genetic and epigenetic changes in human epithelial cells immortalized by telomerase. | exogenous expression of htert, the catalytic component of telomerase, is sufficient for the immortalization of human fibroblasts but insufficient for the immortalization of human foreskin keratinocytes (hfks) and human mammary epithelial cells (hmecs). these latter cell types can overcome senescence by coexpression of htert and human papillomavirus (hpv) e7 or by expression of htert and loss of p16(ink4a) expression, indicating that the retinoblastoma (rb) pathway, along with a telomere maintena ... | 2000 | 10793065 |
| folding of a dimeric beta-barrel: residual structure in the urea denatured state of the human papillomavirus e2 dna binding domain. | the dimeric beta-barrel is a characteristic topology initially found in the transcriptional regulatory domain of the e2 dna binding domain from papillomaviruses. we have previously described the kinetic folding mechanism of the human hpv-16 domain, and, as part of these studies, we present a structural characterization of the urea-denatured state of the protein. we have obtained a set of chemical shift assignments for the c-terminal domain in urea using heteronuclear nmr methods and found region ... | 2000 | 10794423 |
| p53 codon 72 polymorphism does not affect the risk of cervical cancer in patients from northern italy. | a case-control study was performed to investigate the risk of cervical cancer associated with p53 polymorphism at codon 72, encoding either arginine or proline. it has been recently suggested that the arginine isoform increases the susceptibility to invasive cervical cancer; however, data remain controversial. the polymorphism was examined by both allele-specific pcr and rflp analysis in 101 patients with primary cervical cancer and in 140 healthy women of the same age and from the same geograph ... | 2000 | 10794489 |
| differential cell cycle response of nontumorigenic and tumorigenic human papillomavirus-positive keratinocytes towards transforming growth factor-beta1. | human papillomaviruses (hpvs) are causative agents of a number of malignancies in humans, including cervical cancer. their tumorigenic potential is linked to expression of the viral e6/e7 genes which can interfere with normal cell cycle control by targeting p53, p21waf1, p27kip1, and prb. we show here that nontumorigenic and tumorigenic hpv-positive keratinocytes (hpk) exhibit striking differences in the response of cell cycle regulatory genes towards transforming growth factor beta-beta1. treat ... | 2000 | 10794545 |
| analysis of mutations in the urr and e6/e7 oncogenes of hpv 16 cervical cancer isolates from central china. | high rates of cervical cancer have been reported from parts of china and this may reflect a predominance of cervical infection with particularly aggressive human papillomavirus (hpv) variants. this pcr-based investigation of cervical tumours from sichuan province in central china demonstrated an hpv positivity rate of 88%. hpv 16 was most common (21/34, 61%), followed by hpv 18 (3/34, 9%), while types 33, 45, 58 and 59 were each identified in one specimen. sequencing of up to 1349 bases of the 2 ... | 2000 | 10797293 |
| factor analysis of confocal image sequences of human papillomavirus dna revealed with fast red in cervical tissue sections stained with toto-iodide. | to visualize and localize specific dna sequences by fluorescence in situ hybridization, confocal laser scanning microscopy (clsm) and factor analysis of biomedical image sequences (famis). | 2000 | 10800619 |
| pkn binds and phosphorylates human papillomavirus e6 oncoprotein. | the high risk human papillomaviruses (hpvs) are associated with carcinomas of cervix and other genital tumors. previous studies have identified two viral oncoproteins e6 and e7, which are expressed in the majority of hpv-associated carcinomas. the ability of high risk hpv e6 protein to immortalize human mammary epithelial cells has provided a single gene model to study the mechanisms of e6-induced oncogenic transformation. in recent years, it has become clear that in addition to e6-induced degra ... | 2000 | 10809724 |
| human papillomavirus testing as a screening tool for cervical cancer | 2000 | 10815111 | |
| induction of s phase and apoptosis by the human papillomavirus type 16 e7 protein are separable events in immortalized rodent fibroblasts. | the hpv16 e7 oncoprotein neutralizes several cell cycle checkpoints, favouring the entry of quiescent cells into s phase. this activity is mediated in part by association of e7 with the pocket proteins and consequent activation of e2f transcription factors. in addition, hpv16 e7 protein is able to promote apoptosis. in this study we demonstrate that the ability to induce apoptosis is a common property of e7s belonging to both benign and malignant hpv types. the e7-induced apoptosis is mediated b ... | 2000 | 10822378 |
| screening and treatment of sexually transmitted diseases part 2: trichomonas, human papillomavirus infection, and genital herpes simplex virus. | 2000 | 10823973 | |
| induction of apoptosis in human papillomaviruspositive cancer cells by peptide aptamers targeting the viral e6 oncoprotein. | certain types of human papillomaviruses (hpvs) are closely linked to the development of human cancers. herein, it is shown that intracellular targeting of the hpv16 e6 oncoprotein by e6-binding peptide aptamers resulted in the apoptotic elimination of hpv16-positive cancer cells, whereas hpv-negative cells were not affected. these results provide direct experimental evidence that the hpv e6 oncoprotein has antiapoptotic activity in hpv-positive tumor cells that is required for their survival. th ... | 2000 | 10829072 |
| human papillomavirus testing in women with mild cytologic atypia. | to evaluate the efficiency of human papillomavirus (hpv) testing by hybrid capture ii (digene diagnostics inc., silver spring, md) with regard to detecting biopsy-confirmed cervical intraepithelial neoplasia (cin) or high-grade cin in women with mild atypia, compared with the efficiencies of polymerase chain reaction (pcr), southern blot hybridization, and cytology. | 2000 | 10831974 |
| comparison of human papillomavirus types 16, 18, and 6 capsid antibody responses following incident infection. | the relationship between human papillomavirus (hpv) dna in the genital mucosa and serum igg to hpv-16, -18, and -6 was studied in a cohort of 588 college women. among women with incident hpv infections, 59.5%, 54.1%, and 68.8% seroconverted for hpv-16, -18, or -6, respectively, within 18 months of detecting the corresponding hpv dna. transient hpv dna was associated with a failure to seroconvert following incident hpv infection; however, some women with persistent hpv dna never seroconverted. an ... | 2000 | 10837170 |
| [diagnosis and treatment modalities in sinonasal inverted papillomas]. | today, etiology and pathogenesis of sinunasal inverted papillomas (ip) remain unknown. surgical approaches and extension of surgical resections are still controversial. | 2000 | 10838687 |
| detection of human papillomavirus in cholesteatomas. | the presence of human papillomavirus dna in cholesteatoma may have some role in the development of middle ear cholesteatoma as well as in papilloma. in the present study, polymerase chain reaction and in situ hybridization with human papillomavirus (hpv)-6 and -11 dna probes were used to detect the presence of hpv dna in 32 human middle ear cholesteatomas. only one specimen contained hpv-6 dna. although its occurrence may have been coincidental, it is also possible that the hyperproliferative ep ... | 2000 | 10839482 |
| solitary squamous cell papilloma of the lung in a 40-year-old woman with recurrent laryngeal papillomatosis. | a rare case of recurrent respiratory papillomatosis (rrp) is reported with a review of the literature. a 40-year-old japanese woman had suffered from rrp since 1 year of age. she developed a pulmonary squamous papilloma with a thin-walled cavity, which was suspected as being lung carcinoma. the trachea and bronchi around the tumor were intact, and no malignant transformation was present. two types of human papillomavirus, 6 and 16, were detected, both in the laryngeal and pulmonary papillomas by ... | 2000 | 10849335 |
| [papillomavirus infection in healthy women from st.petersburg]. | the study was aimed to evaluate the features of human papillomavirus (hpv) infection in healthy female inhabitants of st. petersburg. 309 attendants of gynecological clinics were recruited for the screening. all participants were of reproductive age and were lacking the clinical or morphological symptoms of hpv-related diseases. pcr revealed hpv-specific sequences in 90 (29%) women. hpv type distribution was similar to the one in usa and europe. hpv-positivity did not correlate with age of femal ... | 2000 | 10853416 |
| immunomodulatory therapy in the management of viral infections in patients with hiv infection. | human immunodeficiency virus (hiv) causes disease by infecting lymphocytes and progressively destroying critical regulatory and effector cells of the immune system, leaving patients vulnerable to a number of bacterial, fungal, and viral infections. facial herpes (herpes simplex virus-1 [hsv-1]), genital herpes (hsv-2), herpes zoster (varicella zoster virus), oral hairy leukoplakia (epstein-barr virus), kaposi's sarcoma (hhv-8), molluscum contagiosum, condyloma acuminata (human papillomavirus [hp ... | 2000 | 10861104 |
| human papillomavirus infection and cervical carcinoma. | 2000 | 10863634 | |
| human papillomavirus type 16 e6 induces self-ubiquitination of the e6ap ubiquitin-protein ligase. | the e6 protein of the high-risk human papillomaviruses (hpvs) and the cellular ubiquitin-protein ligase e6ap form a complex which causes the ubiquitination and degradation of p53. we show here that hpv16 e6 promotes the ubiquitination and degradation of e6ap itself. the half-life of e6ap is shorter in hpv-positive cervical cancer cells than in hpv-negative cervical cancer cells, and e6ap is stabilized in hpv-positive cancer cells when expression of the viral oncoproteins is repressed. expression ... | 2000 | 10864652 |
| the human papillomavirus type 16 e7 oncogene is required for the productive stage of the viral life cycle. | the production of the human papillomavirus type 16 (hpv-16) is intimately tied to the differentiation of the host epithelium that it infects. infection occurs in the basal layer of the epithelium at a site of wounding, where the virus utilizes the host dna replication machinery to establish itself as a low-copy-number episome. the productive stage of the hpv-16 life cycle occurs in the postmitotic suprabasal layers of the epithelium, where the virus amplifies its dna to high copy number, synthes ... | 2000 | 10864676 |
| cutaneous and mucous infections from human papilloma virus: new therapeutical approach. | 2000 | 10876965 | |
| enhanced oncogenicity of human papillomavirus type 16 (hpv16) variants in japanese population. | to investigate whether hpv16 e6 variants carry an elevated risk for cervical cancer in japanese population, we investigated the e6 sequence variation in 40 cervical intraepithelial neoplasias (cins) i-iii and 43 invasive cervical cancers (iccs), all positive for hpv16. hpv16 e6 variants were frequently found in iccs than in cins (88 vs. 65%, p=0.01). the e6 d25e, a rare variant in western countries, was most frequently observed in icc (44%). cin i/ii lesions with hpv16 variants were less likely ... | 2000 | 10880765 |
| consistent high viral load of human papillomavirus 16 and risk of cervical carcinoma in situ: a nested case-control study. | persistent infection with certain types of human papillomavirus (hpv) is believed to be a prerequisite for the development of cervical neoplasia. persistence may depend on certain characteristics, such as viral load, which has so far been given little attention. we investigated the association between hpv 16 viral load and cervical carcinoma in situ. | 2000 | 10881892 |
| efficacy in treatment of subclinical cervical hpv infection without intraepithelial neoplasia: systematic review. | the treatment of the subclinical human papillomavirus (hpv) infection of the uterine cervix is controversial. | 2000 | 10887387 |
| tumor viruses and endogenous retrotransposons in mammary tumorigenesis. | much of our knowledge about the intricate pathways and molecular mechanisms involved in the conversion of a normal mammary epithelial cell to malignancy derives from studies on mammary tumorigenesis induced by the retrovirus mouse mammary tumor virus. in addition, three dna tumor viruses, simian virus 40, polyomavirus, and human papillomavirus, have been instrumental in dissecting the series of steps comprising neoplastic progression of mammary epithelium, particularly with cultured human breast ... | 1996 | 10887480 |
| genome-wide analysis of oral cancer--early results from the cancer genome anatomy project. | the cancer genome anatomy project (cgap) is a large cooperative effort sponsored by the us national institutes of health designed to find, catalog and annotate genes that are expressed during cancer development. in the past 2 years, the cgap has sequenced over 700,000 clones from approximately 140 cdna libraries, resulting in the identification of over 30,000 new human genes. as a first step in applying this project to oral cancer we entered four cell lines--two from oral cancer, one from primar ... | 2000 | 10889913 |
| human papillomavirus and human cytomegalovirus dnas presence in patients suspected of condylomatosis or papillomatosis. | 17 samples of total cell dna isolated from cervical smears from women suspected of condyloma or papilloma were analysed by pcr with appropriate primers, in order to establish the presence of viral dnas (hpv and/or hcmv). hpv dna was found in seven cases, and so was for hcmv dna. only in three cases a coinfection was present. the rflp allowed to specify the involvement of hpv6 in 3 cases suspected of condyloma and in one suspected of papilloma; the other three hpv positive samples had another gen ... | 1998 | 10892429 |
| the role of human papillomavirus in gynecological diseases. | papanicolaou (pap) smears of the cervix and biopsies of the cervix, vagina, vulva, and penis are a substantial portion of the work load of any anatomic pathology laboratory. the importance of samples from these sites is highlighted by the fact that their most common disease is dysplasia, which is sexually transmitted and a recognized risk factor for carcinoma. human papillomavirus (hpv) is an essential co-factor for the development of lower genital tract dysplasia. molecular testing for hpv has ... | 2000 | 10894184 |
| the restricted cellular host range of human herpesvirus 8. | a selection of primary and transformed cell types were evaluated for their susceptibility to infection with human herpesvirus 8 (hhv-8)/kaposi's sarcoma-associated herpesvirus. | 2000 | 10894276 |
| anal verrucous carcinoma and penile condylomata acuminata. | a 50-year-old man presented with recurrent tumorous lesions on the penis and the anal region. the anal lesion was histologically diagnosed as verrucous carcinoma (vc) and the penile lesions were in line with condylomata acuminata. samples taken from tumors of both sites were human papillomavirus (hpv) dna positive. two of them taken from the penis and the perianal region scored hpv dna 6 positive by using polymerase chain reaction and the southern blot method. treatment of both vc and condylomat ... | 2000 | 10894964 |
| variants of the long control region of human papillomavirus type 16. | expression of the human papillomavirus (hpv) e6 and e7 oncogenes is regulated on the transcriptional level by specific protein-binding sites contained in the viral long control region (lcr). sequence changes within the lcr region may have an impact on the transcription of viral oncogenes, possibly resulting in differences in the oncogenic potential of the virus. the present study was designed to determine the sequence variability of the lcr of hpv 16 and to assess whether certain lcr variants do ... | 2000 | 10899654 |
| chromium(vi) induces p53-dependent apoptosis in diploid human lung and mouse dermal fibroblasts. | some forms of hexavalent chromium [cr(vi)] are known to cause damage to respiratory-tract tissue and dna and are thought to be human lung carcinogens. in general, cr(vi) is mutagenic and carcinogenic at doses that also evoke some cell death, and we previously showed that the predominant mode of death is apoptosis. because p53 has been shown to initiate apoptosis after genotoxic insults, the objective of these experiments was to determine whether p53 is activated in and necessary for apoptosis of ... | 2000 | 10900468 |
| leaky scanning is the predominant mechanism for translation of human papillomavirus type 16 e7 oncoprotein from e6/e7 bicistronic mrna. | human papillomaviruses (hpv) are unique in that they generate mrnas that apparently can express multiple proteins from tandemly arranged open reading frames. the mechanisms by which this is achieved are uncertain and are at odds with the basic predictions of the scanning model for translation initiation. we investigated the unorthodox mechanism by which the e6 and e7 oncoproteins from human papillomavirus type 16 (hpv-16) can be translated from a single, bicistronic mrna. the short e6 5' untrans ... | 2000 | 10906182 |
| identification of domains of the human papillomavirus type 11 e1 helicase involved in oligomerization and binding to the viral origin. | the e1 helicase of papillomavirus is required, in addition to host cell dna replication factors, during the initiation and elongation phases of viral episome replication. during initiation, the viral e2 protein promotes the assembly of enzymatically active multimeric e1 complexes at the viral origin of dna replication. in this study we used the two-hybrid system and chemical cross-linking to demonstrate that human papillomavirus type 11 (hpv11) e1 can self-associate in yeast and form hexamers in ... | 2000 | 10906188 |
| papillary squamous cell carcinoma of the uterine cervix: report of three cases and a review of its classification. | papillary squamous cell carcinoma (scc) of the uterine cervix has been defined as a malignant squamous cell lesion characterized by a papillary architecture with fibrovascular cores and moderate to severe dysplasia devoid of frank keratinization and koilocytic change. papillary scc should be histopathologically delineated from other rare variants of scc with papillary features including verrucous and condylomatous carcinoma and the recently recognized (squamo-)transitional cell carcinoma of the ... | 2000 | 10907171 |
| persistence of human papillomavirus type 16 infection is associated with lack of cytotoxic t lymphocyte response to the e6 antigens. | our cross-sectional study suggested that cytotoxic t lymphocyte (ctl) responses have a protective effect in squamous intraepithelial lesion (sil) development. more ctl responses in women with human papillomavirus type 16 (hpv 16) infection without sils than with sils were detected. in the current longitudinal study, the role of ctl in clearing hpv 16 infection in women without sils was investigated. women with hpv 16 infection (n=51) were enrolled, along with hpv 16-negative control women (n=3). ... | 2000 | 10915094 |
| case-control study of plasma folate, homocysteine, vitamin b(12), and cysteine as markers of cervical dysplasia. | an association between b-complex vitamins and related compounds with the development of cervical neoplasia is biologically plausible, yet to the authors' knowledge epidemiologic investigations of these potential biomarkers are limited. | 2000 | 10918169 |
| p53 polymorphism in codon 72 and risk of human papillomavirus-induced cervical cancer: effect of inter-laboratory variation. | an association between codon-72 p53 polymorphism and risk of human papillomavirus (hpv)-induced cervical cancer has been found recently, but it has been difficult to replicate. in this study, we assess the impact of inter-laboratory variation in p53 genotyping on the validity of the proposed association. dna specimens were randomly selected from 54 invasive, squamous cell carcinoma cases, 52 hpv-negative, and 39 hpv-positive controls from a previous case-control study in brazil. codon-72 polymor ... | 2000 | 10918193 |
| cost-effectiveness of the conventional papanicolaou test with a new adjunct to cytological screening for squamous cell carcinoma of the uterine cervix and its precursors. | to estimate costs and outcomes of conventional annual papanicolaou (pap) test screening compared with biennial pap test plus speculoscopy (pps) screening for cervical neoplasms. | 2000 | 10927709 |
| modulation of the epidermal growth factor receptor by the human papillomavirus type 16 e5 protein in raft cultures of human keratinocytes. | it has been shown that the e5 protein of the human papillomavirus type 16 modulates epidermal growth factor receptor downregulation in monolayer cultures of human keratinocytes and mouse fibroblasts. we have now analysed the effect of this protein on the expression, the distribution and the activation of egf receptors in raft cultures derived from an e5-transfected human keratinocyte cell line. the epithelia generated in these cultures were stratified and exhibited suprabasal expression of cytok ... | 2000 | 10928456 |
| human papillomavirus in venous ulcers with and without squamous cell carcinoma. | malignant transformation of chronic wounds is a well-known, albeit rare, phenomenon. we examined archival paraffin blocks of samples of squamous cell carcinoma (scc) in chronic venous leg ulcers previously taken from 23 patients and of chronic noncancerous venous leg ulcers from 35 patients for the presence of human papillomavirus (hpv) dna. the methods used were the polymerase chain reaction (pcr) with gp05+/06+ (mucosal) and nested pcr with cp65/70 and cp66/69 (ev-associated) primers. a subseq ... | 2000 | 10929767 |
| inhibition of human papillomavirus type 16 gene expression by nordihydroguaiaretic acid plant lignan derivatives. | several methylated derivatives of a plant lignan, nordihydroguaiaretic acid (ndga) were found to be potent anti-viral agents by suppressing sp1 regulated transcription within the sexually transmitted viruses human immunodeficiency virus (hiv) and herpes simplex virus (hsv). a prominent sp1 dna binding site within many human papillomavirus (hpv) promoters has been noted to play an active role in hpv gene expression. in this report it is shown that the three ndga derivatives, mal.4, m(4)n, and tet ... | 2000 | 10930643 |
| human papillomavirus types 56 and 59 in novel cervical cancer cell lines. | 2000 | 10937347 | |
| differential behavior of vegf receptor expression and response to tnp-470 in two immortalized human endothelial cell lines. | angiogenesis consists of endothelial cell proliferation, migration and tube formation. it is useful to investigate endothelial cell behavior using immortalized endothelial cell lines. we characterized cell growth property, growth factor dependency and response to angioinhibitory drugs; tnp-470, staurosporine, radicicol and genistein, using human umbilical vein endothelial cells (huvecs) immortalized by human papilloma virus (hpv)-16 e6-e7, named huvecs/e6-e7, and huvecs/e6-e7 transformed by v-ki ... | 2000 | 10938394 |
| identification in humans of hpv-16 e6 and e7 protein epitopes recognized by cytolytic t lymphocytes in association with hla-b18 and determination of the hla-b18-specific binding motif. | human papilloma virus type 16 (hpv-16) is the hpv most frequently associated with cervical carcinoma in humans. for the prevention or treatment of cervical carcinoma, the e6 and e7 oncoproteins appear to be good targets for vaccine-induced cytotoxic t lymphocytes (ctl). lipopeptide vaccination is an efficient way of stimulating cellular responses. however, to synthesize effective lipopeptides, it is necessary to define which epitopes are immunogenic. in this study we first determined that peptid ... | 2000 | 10940919 |
| sexually transmitted infection and teenage sexuality. | the twin epidemics of sexually transmitted infection and nonmarital pregnancy and their consequences are two of the most significant issues faced by americans today. approximately 900,000 teenagers become pregnant each year. research indicates that these pregnancies often limit future educational and economic opportunities for mother, father, and child. sexually transmitted infections also pose a major threat to adolescents. for example, during a 3-year study of sexually active women at a major ... | 2000 | 10942466 |
| the human papillomavirus type 16 e6 and e7 oncoproteins cooperate to induce mitotic defects and genomic instability by uncoupling centrosome duplication from the cell division cycle. | loss of genomic integrity is a defining feature of many human malignancies, including human papillomavirus (hpv)-associated preinvasive and invasive genital squamous lesions. here we show that aberrant mitotic spindle pole formation caused by abnormal centrosome numbers represents an important mechanism in accounting for numeric chromosomal alterations in hpv-associated carcinogenesis. similar to what we found in histopathological specimens, hpv-16 e6 and e7 oncoproteins cooperate to induce abno ... | 2000 | 10944189 |
| association between activated k-ras and c-erbb-2 oncogenes with "high-risk" and "low-risk" human papilloma virus types in preinvasive cervical lesions. | clinical and epidemiological data have linked cervical cancer to the human papilloma virus (hpv) infection. however, the presence of hpv infection alone is not enough to cause tumorigenesis, suggesting a role for additional host-cell genetic factors. the aim of the present work was to study the association of k-ras and c-erbb-2 mutations in cervical tissue samples with different grades of dysplasia and infected with hpv-6 ("low-risk" type) or hpv-16 and hpv-18 ("high-risk" types). negative hpv-d ... | 2000 | 10946249 |
| penile hybrid verrucous-squamous carcinoma associated with a superficial inguinal lymph node metastasis. | a 62-year-old japanese man who presented with penile carcinoma is reported. the initial exophytic neoplasm excised from the coronal sulcus and prepuce on the abdominal side of the penis was diagnosed histologically as verrucous carcinoma. twenty-six months after the primary operation, an epithelial neoplasm recurred within the scar of the primary operation. the neoplasm histologically showed verrucous carcinoma and multiple invasive foci of conventional squamous cell carcinoma in the advancing e ... | 2000 | 10949460 |
| anal squamous intraepithelial lesions in human immunodeficiency virus-positive men and women. | studies from the era prior to the introduction of highly active antiretroviral therapy (haart) have shown that the prevalence of anal human papillomavirus (hpv) infection and anal squamous intraepithelial lesions (asil) was very high among human immunodeficiency virus (hiv)-positive homosexual men, and to a lesser extent, among hiv-negative homosexual men. prospective data also show that the incidence of high-grade asil (hsil), the putative invasive cancer precursor lesion, was high among both h ... | 2000 | 10950374 |
| erythroplasia of queyrat: coinfection with cutaneous carcinogenic human papillomavirus type 8 and genital papillomaviruses in a carcinoma in situ. | erythroplasia of queyrat is a carcinoma in situ that mainly occurs on the glans penis, the prepuce, or the urethral meatus of elderly males. up to 30% progress to squamous cell carcinoma. the cause of erythroplasia of queyrat is largely unknown. human papillomavirus type 16 dna has previously been detected only in very few distinctly characterized patients. we have investigated 12 paraffin-embedded biopsies from eight patients with penile erythroplasia of queyrat and control biopsies of inflamma ... | 2000 | 10951274 |
| connexin 43 expression is downregulated in raft cultures of human keratinocytes expressing the human papillomavirus type 16 e5 protein. | a decrease in gap junction-mediated cell-to-cell communication has previously been observed in monolayer cultures of human keratinocytes (hacat cells) expressing the human papillomavirus type 16 e5 (hpv16 e5) gene and attributed to the reduced phosphorylation of connexin 43, the most abundant connexin in hacat cells. in line with this observation, we have now analyzed the effect of hpv16 e5 on connexin 43 expression in raft cultures produced by transfected hacat cells. these keratinocytes transc ... | 2000 | 10955727 |
| iga antibodies to the 27-kda heat-shock protein in the genital tracts of women with gynecologic cancers. | heat-shock proteins promote cell survival under adverse environmental conditions. synthesis of the 27-kda (hsp27), 70-kda (hsp70), and 90-kda (hsp90) heat-shock proteins is increased in malignantly transformed cells and has been associated with tumor proliferation, metastasis, and resistance to chemotherapeutic agents. the increased expression of heat-shock proteins and their association with tumor-specific antigens may result in local immunity to the heat-shock proteins. we examined the occurre ... | 2000 | 10956393 |
| detection of human papillomavirus in laryngeal squamous dysplasia and carcinoma. an in situ hybridization and signal amplification study. | we examined the presence of human papillomavirus (hpv) dna in 65 cases of laryngeal squamous dysplasia and carcinomas using in situ hybridization with signal amplification in paraffin sections. hybridization was performed with biotinylated dna probes for hpv 6/11, 16/18, 31/33 and wide-spectrum hpv (6, 11, 16, 30, 31, 45, 51 and 52). hpv dna was found in 7 cases of the total sample (10.7%); it was also found in 4 out of 45 (8.8%) cases of invasive carcinoma and in 5 out of 33 (15.5%) cases of sq ... | 2000 | 10958409 |
| uncoupling between phenotypic senescence and cell cycle arrest in aging p21-deficient fibroblasts. | irreversible g(1) arrest in senescent human fibroblasts is mediated by two inhibitors of cyclin-dependent kinases (cdks), p21(cip1/sdi1/waf1) and p16(ink4a). to determine the physiological and molecular events that specifically require p21, we studied senescence in human diploid fibroblasts expressing the human papillomavirus type 16 e6 oncogene, which confers low p21 levels via enhanced p53 degradation. we show that in late-passage e6 cells, high cdk activity drives the cell cycle, but populati ... | 2000 | 10958672 |
| prediction of cervical intraepithelial neoplasia grade 2-3 using risk assessment and human papillomavirus testing in women with atypia on papanicolaou smears. | to determine whether human papillomavirus (hpv) dna testing and risk assessment can predict cervical intraepithelial neoplasia (cin) 2-3 on biopsies in women with atypia on papanicolaou smears. | 2000 | 10960635 |
| european course on hpv associated pathology: guidelines for primary care physicians for the diagnosis and management of anogenital warts. | the european course on hpv associated pathology (echpv) was founded in 1990 by a group of clinicians, pathologists, and virologists to teach important principles for the practice and management of human papillomavirus (hpv) disease to gynaecologists, dermatologists, and other medical disciplines. these guidelines are intended to assist the practice of primary care physicians for diagnosis and treatment of anogenital warts. | 2000 | 10961190 |
| human papillomavirus type 16 e6/e7-specific cytotoxic t lymphocytes in women with cervical neoplasia. | infection with oncogenic human papillomavirus (hpv) types is associated with the development of cervical neoplasia (cin). the e6 and e7 oncoproteins are constitutively expressed in these lesions and are therefore putative targets for the immune response against hpv. the relation between hpv 16-specific memory cytotoxic t-cell precursor (mctlp) activity to both oncoproteins and the natural course of cervical dysplasia was analyzed in 38 patients participating in a nonintervention cohort study of ... | 2000 | 10962445 |
| brca1 activation of the gadd45 promoter. | breast cancer susceptibility gene brca1 has been implicated in the control of gene regulation and such regulated genes are thought to mediate the biological role of brca1. overexpression of brca1 induces gadd45, a p53-regulated and stress-inducible gene. however, the molecular mechanism by which brca1 induces the expression gadd45 remains unclear. in this report, we have shown that the gadd45 promoter is strongly activated following expression of wild-type brca1. in contrast, both the tumor-deri ... | 2000 | 10962562 |
| casein kinase ii phosphorylation of the human papillomavirus-18 e7 protein is critical for promoting s-phase entry. | the human papillomavirus type 18 e7 protein subverts the prb/e2f pathway to promote s-phase reentry by postmitotic, differentiated primary human keratinocytes in support of viral dna amplification. we prepared a panel of hpv-18 e7 mutations in prb binding or in casein kinase ii (ckii) phosphorylation. our results showed that the ability of e7 binding to prb correlated with the activation of dna polymerase alpha or cyclin e to various extents in differentiated keratinocytes of organotypic culture ... | 2000 | 10965847 |
| immunotherapy of gynaecological high-risk human papilloma virus infection with human leukocyte ultrafiltrate. | in this preliminary presentation the outcome of women following systemic treatment of cervical human papilloma virus (hpv) with a leukocyte ultrafiltrate is reported. | 2000 | 10968593 |
| detection of human papillomavirus type 16 dna in consecutive genital samples does not always represent persistent infection as determined by molecular variant analysis. | persistent human papillomavirus (hpv) infection of the uterine cervix is a risk factor for progression to high-grade squamous intraepithelial lesions. detection in consecutive genital samples of hpv-16 dna, a frequently encountered hpv type, may represent persistent infection or reinfection. we undertook a study using pcr-single-strand conformation polymorphism (sscp) analysis and sequencing of pcr products (pcr-sequencing) to determine if consecutive hpv-16-positive samples contained the same h ... | 2000 | 10970388 |
| regulation of the 26s proteasome by adenovirus e1a. | we have identified the n-terminus of adenovirus early region 1a (ade1a) as a region that can regulate the 26s proteasome. specifically, in vitro and in vivo co-precipitation studies have revealed that the 19s regulatory components of the proteasome, sug1 (s8) and s4, bind through amino acids (aa) 4-25 of ad5 e1a. in vivo expression of wild-type (wt) ade1a, in contrast to the n-terminal ade1a mutant that does not bind the proteasome, reduces atpase activity associated with anti-s4 immunoprecipita ... | 2000 | 10970867 |
| preventive and therapeutic vaccines for human papillomavirus-associated cervical cancers. | 'high risk' genotypes of the human papillomavirus (hpv), particularly hpv type 16, are the primary etiologic agent of cervical cancer. thus, hpv-associated cervical malignancies might be prevented or treated by induction of the appropriate virus-specific immune responses in patients. sexual transmission of hpv may be prevented by the generation of neutralizing antibodies that are specific for the virus capsid. in ongoing clinical trials, hpv virus-like particles (vlps) show great promise as prop ... | 2000 | 10971133 |
| requirement of wild-type p53 protein for maintenance of chromosomal integrity. | chromosomal double-strand breaks (dsbs) occurring in mammalian cells can initiate genomic instability, and their misrepairs result in chromosomal deletion, amplification, and translocation, common findings in human tumors. the tumor-suppressor protein p53 is involved in maintaining genomic stability. in this study, we demonstrate that the deficiency of wild-type p53 protein may allow unrepaired dsbs to initiate chromosomal instability. the human lymphoblastoid cell line tk6-e6 was established by ... | 2000 | 10972990 |
| new technologies in cervical cancer precursor detection. | the current literature reflects three routes toward improving cervical cancer screening. the first is to improve the test qualities of cytology-based screening. the use of liquid-based cytology and computerized analysis of papanicolaou tests are examples of attempts at this approach. secondly, through various combinations of parallel or sequential tests, either the sensitivity or the specificity of a given test could be improved depending on the tests chosen and the order in which they were perf ... | 2000 | 10975554 |
| molecular identification of 7 human papillomavirus types in recurrent respiratory papillomatosis. | recurrent respiratory papillomatosis (rrp) is the most frequent benign neoplasm in childhood; it originates as a mild dysphonia and results in asphyxia. the rrp has been associated with an infection caused by human papillomavirus (hpv), mainly types 6 and 11, the latter being associated with more severe rrp. | 2000 | 10979126 |
| association of vitamin a deficiency with cervical squamous intraepithelial lesions in human immunodeficiency virus-infected women. | to explore the relationship between vitamin a (retinol) deficiency and cervical squamous intraepithelial lesions (sils) in human immunodeficiency virus (hiv)-infected women, we measured serum retinol concentrations in 1314 women enrolled in the women's interagency hiv study and correlated the results with concurrent cervical cytology. at the baseline visit, 204 (15.5%) of the 1314 patients had retinol concentrations consistent with deficiency (<1.05 micromol/l). analysis of papanicolaou smears s ... | 2000 | 10979903 |
| [human papillomavirus and the development of cervical cancer: concept of carcinogenesis]. | infection with high risk human papillomavirus (hrhpv) plays a central aetiological role in cervical cancer. still, cervical carcinogenesis is a multistep process which requires other events in addition to hrhpv infection. recent data have resulted in the following concept of cervical carcinogenesis: hrhpv infects normal squamous epithelium. in most cases this will not lead to a lesion or at worst give rise to a regressing low grade cervical intraepithelial neoplasia (cin). both phenomena involve ... | 2000 | 10981234 |
| telomerase activity in hpv-associated vulvar vestibulitis. | to find a possible correlation between telomerase activity, mean telomere length and human papillomavirus (hpv) presence and type in vulvar vestibulitis. | 2000 | 10986682 |
| pregnancy-associated invasive squamous cell carcinoma of the vulva in a 28-year-old, hiv-negative woman. a case report. | the incidence of invasive squamous cell carcinoma of the vulva in women under 40 years of age has been increasing, particularly in association with human papillomavirus. invasive vulvar carcinoma is rare in women under 30, as is an association with pregnancy. we report on a 28-year-old woman who was diagnosed with invasive squamous cell carcinoma of the vulva during pregnancy. | 2000 | 10986685 |
| human papillomavirus infection in danish female sex workers. decreasing prevalence with age despite continuously high sexual activity. | the prevalence of the human papillomavirus (hpv) has been found to decrease with age, but whether this applies to all populations is not clear. knowledge about the generalizability of this age dependency is important to understand the natural history of the infection, but may also have implications in relation to the use of hpv testing in cervical cancer screening. | 2000 | 10987448 |
| a population-based five-year follow-up study of cervical human papillomavirus infection. | the purpose of this study was to determine the long-term tendency for cervical human papillomavirus infections to persist in the general population. | 2000 | 10992174 |
| up-regulation of epidermal growth factor-receptors (egf-r) by nicotine in cervical cancer cell lines: this effect may be mediated by egf. | over-expression of epidermal growth factor-receptors (egf-r) has been described in a variety of cancers, including cervical cancer. nicotine may increase cellular proliferation rates through a mechanism involving egf or egf-r. in this study, we ascertain the effect of egf antibodies on nicotine-enhanced proliferation rates in two cervical cancer cell lines. | 2000 | 10994639 |
| human papillomavirus-associated cancers in patients with human immunodeficiency virus infection and acquired immunodeficiency syndrome. | human papillomavirus (hpv)-associated anogenital malignancies occur frequently in patients with human immunodeficiency virus (hiv) infection and the acquired immunodeficiency syndrome (aids). the purpose of our study was to determine if the high frequency of these cancers is due to lifestyle factors associated with both hpv and hiv infections or to immunosuppression following hiv infection. | 2000 | 10995805 |
| amplification with molecular beacon primers and reverse line blotting for the detection and typing of human papillomaviruses. | a novel method for the detection and typing of human papillomavirus (hpv) was developed using molecular beacon primers. the method is based on the use of hpv-specific primers containing a hairpin loop structure in which fluorescent donor and quencher groups are held in close proximity such that fluorescence is quenched. amplification of the target sequence results in the opening of the loop and the resulting fluorescence can be detected on a sequence detector system (sds) 7700 (applied biosystem ... | 2000 | 10996637 |
| a recombinant vaccinia virus containing the papilloma e2 protein promotes tumor regression by stimulating macrophage antibody-dependent cytotoxicity. | human papillomavirus infection is associated with cervical cancer. the e6 and e7 papillomavirus proteins are normally required for the maintenance of the malignant phenotype. expression of these proteins in infected cells is negatively regulated by the binding of the papilloma e2 protein to the long terminal control region of the papilloma virus genome. the e2 protein can also promote cell arrest and apoptosis in hela cells. therefore, it is clear that this protein has the potential of inhibitin ... | 2000 | 10999461 |
| usefulness of human papilloma virus testing in the screening of cervical cancer precursor lesions: a retrospective study in 314 cases. | to assess the usefulness of human papilloma virus (hpv) typing for predicting pre-malignant and malignant cervical lesions. | 2000 | 11000508 |
| validity of adolescent and young adult self-report of papanicolaou smear results. | to assess the validity of adolescent and young adult report of papanicolaou smear results and to determine sociodemographic, cognitive, and behavioral factors associated with incorrect reporting. | 2000 | 11004371 |