Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a selenium-containing catalytic antibody with type i deiodinase activity. | acting as a mimic of type i deiodinase (di), a selenium-containing catalytic antibody (se-4c5) prepared by converting the serine residues of monoclonal antibody 4c5 raised against thyroxine (t4) into selenocysteines, can catalyze the deiodination of t(4) to 3,5,3'-triiodothyronine (t(3)) with dithiothreitol (dtt) as cosubstrate. investigations into the deiodinative reaction by se-4c5 revealed the relationship between the initial velocity and substrate concentration was subjected to michaelis-men ... | 2001 | 11355872 |
| estradiol up-regulates antiapoptotic bcl-2 messenger ribonucleic acid and protein in tumorigenic ovarian surface epithelium cells. | most epithelial ovarian tumors appear to arise from the ovarian surface epithelium (ose). even though it has been suggested that estrogen may be associated with ovarian tumorigenesis, the exact role of estrogen in the regulation of apoptosis in neoplastic ose cells remains uncertain. immortalized ose (iose) cell lines were generated from human normal ose. these cell lines represent early neoplastic (iose-29), tumorigenic (iose-29ec), and late neoplastic (iose-29ec/t4 and iose-29ec/t5) transforma ... | 2001 | 11356682 |
| [epidemiological survey for hemolytic streptococci isolated from children in tokyo]. | during the 20-year period between 1979-1998, a total of 4,176 strains of hemolytic streptococci have been isolated from 20,118 healthy primary school children and little children in tokyo metropolitan (tokubetsuku, tama and tosho). culture of throat swabs every november and the following february during the 20-year period were made and serological grouping and typing for isolates were done by t agglutination method. the results were as follows. 1) serological group of hemolytic streptococci isol ... | 2001 | 11357322 |
| scintigraphic evaluation of primary congenital hypothyroidism: results of the greek screening program. | the greek screening program for primary congenital hypothyroidism was initiated in 1979. by early 2000, thyrotropin measurements had been performed in 1,976,719 newborns, using dried blood spots obtained by heel prick. among these children, 584 were diagnosed with congenital hypothyroidism (incidence: 1/3,384 births) and were given l-thyroxine (l-t4) replacement therapy. in order to further evaluate and classify the children as having either an aplastic (at) or an ectopic thyroid gland (et) or a ... | 2001 | 11357505 |
| soluble e-cadherin is a valid prognostic marker in gastric carcinoma. | gastric cancer remains a major cause of cancer mortality globally but no good prognostic tumour marker is available. soluble fragment of e-cadherin protein has been reported to increase in the sera of patients with cancer and recently was found to be elevated in 67% of patients with gastric cancer. | 2001 | 11358900 |
| reversal of lidocaine with epinephrine epidural anesthesia using epidural saline washout. | prolonged motor and sensory block following epidural anesthesia can be associated with extended postoperative care unit stays and patient dissatisfaction. previous studies have demonstrated a more rapid motor recovery following the administration of epidural crystalloids in patients who had received plain bupivacaine and lidocaine epidural anesthesia. however, epinephrine is commonly added to local anesthetics to improve the quality and prolong the duration of the epidural block. the objective o ... | 2001 | 11359224 |
| tt virus (ttv) loads associated with different peripheral blood cell types and evidence for ttv replication in activated mononuclear cells. | tt virus (ttv) loads associated with the peripheral blood cells of seven patients known to carry the virus in plasma were investigated by real-time pcr. whereas red cells/platelets were uniformly negative, six and four patients yielded positive peripheral blood mononuclear cells (pbmcs) and polymorphonuclear leukocytes, respectively, but viral titres were generally low. fractionation of pbmcs into monocyte- and b, t4, and t8 lymphocyte-enriched subpopulations showed no pattern in the viral loads ... | 2001 | 11360252 |
| effect of exogenous thyroid-stimulating hormone on thyroid papillary carcinoma cells in tissue culture. | to determine the prognostic value of thyroid suppression therapy in patients with thyroid carcinoma, we studied the effect of thyroid-stimulating hormone (tsh) on the morphology, proliferation rate, and the t3, t4 production rate of primary thyroid carcinoma cells in culture. | 2001 | 11360310 |
| proliferative capacity in head and neck cancer. | shortening of overall treatment time (accelerated radiotherapy) appears to result in an increase of the efficacy of irradiation. in this study, we compared the proliferative capacity between tumors originating in different sites of the head and neck region. tumors with a large proliferating capacity might, theoretically, benefit most from accelerated radiotherapy. | 2001 | 11360311 |
| [prescription of thyroid function tests in adults as a function of clinical circumstances and of patient management]. | a group of experts composed of clinicians and biologists in 7 university hospitals in the aquitaine and paris regions working within the framework of the coordination committee for clinical evaluation and quality in aquitaine (ccecqa) have developed prescription guidelines for thyroid function tests to be performed in adults. the goal was to determine strategies for prescribing thyroid hormones (t3 and t4) and thyroid stimulating hormone (tsh) to screen for thyroid function disorders and follow ... | 2001 | 11360733 |
| effect of climate on the seasonal endocrine profile of native and crossbred sheep under semi-arid conditions. | thirty-four 2-year-old ewes, of which 17 were native (chokla) and 17 were avivastra (chokla 67.5% x 32.5% rambouillet) breeds of similar body weights, were selected. both groups were maintained under the same management conditions with 8 hours daily grazing. in addition to grazing, each ewe received 300 g/day of a concentrate mixture comprising crude protein 20% and 70% total digestible nutrients. the ewes of both breeds were not mated during the study. the daily environmental parameters were re ... | 2001 | 11360802 |
| effect of pre- and post-partum feed supplementation on the productive and reproductive performance of grazing primiparous brahman cows. | this experiment studied the combined effect of pre- and post-calving feed supplementation on the productive and reproductive performance of primiparous brahman cows. the animals were 4 years old (420 kg live weight) and pregnant, and grazed rotationally on stargrass (cynodon nlemfuensis) pastures. supplementation was given before and after (t1, n = 15), only before (t2, n = 15) or only after (t3, n = 16) calving, and there was an unsupplemented control (t4, n = 16). the supplement (1.5 kg/animal ... | 2001 | 11360803 |
| functional analysis of the phage t4 holin in a lambda context. | phage lambda hybrids were constructed by inserting the t gene of phage t4 in place of the lambda holin gene, s. induction of the hybrid phage resulted in lysis that was just as abrupt as, but occurred much earlier in the vegetative cycle than, that obtained with lambda, indicating that t is indeed a holin gene. moreover, it was possible to impose lysis inhibition (lin) on induction of the hybrid phage, but not of the parental lambda phage, by superinfection with lin-competent t4. the imposition ... | 2001 | 11361346 |
| thyroid dysfunction in hiv-infected children: is l-thyroxine therapy beneficial? | to assess thyroid function in symptomatic hiv-infected infants and children with failure to thrive and to evaluate clinical response in patients with hyperthyrotropinemia to levothyroxine. | 1996 | 11361500 |
| treatment for hiv-related inflammation. | a new 4-month long study is testing the combination of hydroxychloroquine and azt in hiv-positive people. hydroxychloroquine has been used for treating malaria, rheumatoid arthritis, and lupus. participants must be 18 years old or older with a t4 cell count between 200 and 500. two groups will be studied, one using both drugs, and the other using azt only. | 1995 | 11362590 |
| new protease inhibitor studies. | three studies involving the merck protease inhibitor mk-639 are enrolling subjects. these studies involve combining mk-639 with azt in people with t4 cell counts between 50 and 500, with 3tc and azt in people with t4 cell counts between 50 to 400, and with 3tc and azt in people with t4 cell counts under 50. to participate, a person must be 18 years or older and have blood test results in the normal range. persons with a history of elevated, indirect bilirubin will not be allowed to participate. | 1995 | 11362591 |
| about interleukin-2. | interleukin 2 (il-2) is a cytokine that helps boost the body's immune system by causing t4 cells to proliferate, or increase in numbers. il-2 is an approved drug, but not for treatment of aids or hiv-related conditions. il-2 causes hiv growth, although growth rates appear to eventually slow down. researchers are experimenting with low-dose il-2 given by subcutaneous injection to see if the rise in t4 counts will restore the normal immune system function. three new studies combining il-2 with two ... | 1995 | 11362594 |
| interleukin-2 studies. | four interleukin-2 (il-2) studies open to aids patients are outlined. the studies involve a comparison of il-2, thalidomide, and anti-tnf monoclonal antibody; subcutaneous il-2 injections for the treatment of hiv-infected people with over 500 t4 cells counts; intravenous il-2 for treating hiv-infected people with 200 to 500 t4 cells; and subcutaneous injections of il-2 for the treatment of hiv infection in asymptomatic individuals with t4 cells between 200 and 500. | 1995 | 11362595 |
| protease inhibitors. | protease inhibitors, a new class of anti-hiv medications, inhibit the actions of hiv protease, a compound necessary for proper hiv replication. when the actions of this protease are blocked, defective copies of hiv are made or new virus is not produced. these new medications are less damaging to cells than nucleoside analogues (such as azt and 3tc) because their activities are targeted and more specific. research has shown that protease inhibitors reduce the amount of measurable hiv virus in the ... | 1995 | 11362753 |
| merck expanded access program august 11, 1995 deadline. | people with fewer than 50 t4 cells may register before august 11, 1995 to be considered for crixivan, a protease inhibitor made by merck. the registration number is 1-800-497-8383. due to limited drug supply, a lottery will be necessary if more than 1,100 people apply. potential participants must be at least 18 years of age; are permitted to have taken/be taking 3tc, d4t, ddi, ddc or azt; must have more than 50,000 platelets; may not have acute hepatitis; and may not be pregnant. those not selec ... | 1995 | 11362754 |
| another option for less than 50 t4 cells. | people with fewer than 50 t4 cells may be eligible to participate in a clinical trial examining three drugs administered singly or in combination. the drugs are crixivan (mk-639), azt, and 3tc. participants will receive either azt plus 3tc; crixivan, a protease inhibitor; or crixivan plus azt plus 3tc. participants must have taken and tolerated azt for more than six months at the standard dose. azt-naive individuals are eligible for a special, open-label arm of the study. | 1995 | 11362755 |
| new protease inhibitor studies. | several drug companies are testing different brands of protease inhibitors. two new york studies are enrolling people with t4 cell counts between 150 and 500, and between 100 and 500. the first trial is studying saquinavir in combination with azt and 3tc. the second is testing a new soft gelatin capsule formulation of saquinavir. all participants will be able to continue taking study medications after the trials. | 1995 | 11363020 |
| [hiv infection]. | questions and answers about hiv infection are explained using a boxing analogy, with t4 and t8 cells attacking the opponent, hiv-antibodies. how b cells and lymph nodes attack the protein exterior of the hiv virus is described. viral rna combines with the cell's dna to allow the virus to reproduce. it remains there until the cell overflows, reappearing in the blood as the victim notices the first symptoms of aids. | 1995 | 11363196 |
| [which is the strategy? the latest in the use of antiretrovirals]. | the four main antiretroviral drugs, or nucleoside analogs, used in aids treatment are azt, ddi, ddc and d4t. azt is generally prescribed first, adding combinations of ddc of ddi if resistance or intolerance appear. researchers are determining, in vitro, the effects of combining three of these drugs to significantly reduce the reproduction of hiv. the combination of azt and 3tc in studies has produced a potent antiretroviral combination, showing prolonged suppression of hiv production, and an inc ... | 1995 | 11363205 |
| [interleukin 2. how to reconstruct the immune system]. | interleukin-2 (il-2), an immune system protein, raised t-cell levels in laboratory experiments. experiments with this drug began in 1982 for cancer patients, and 1983 for aids patients. t4 cells direct the operations of the immune system. a healthy person has 800 to 1,200 t4 cells per cubic millimeter. an aids patient with a severely impaired immune system may have below 200 t-cells. one study involved 25 persons, 10 who had more than 200 t-cells per cubic millimeter in their blood, and 15 who h ... | 1995 | 11363212 |
| combination therapy advances. | a new class of drugs, protease inhibitors, has been shown to help prevent disease progression and death in people with low t4 cell counts. a combination treatment reduces the amount of hiv in the body, and also raises t4 cell counts even in people who are already taking azt. another protease inhibitor, when taken together with other anti-hiv drugs, dramatically reduces the amount of hiv in the blood. deciding which combination to take depends on many factors, such as side effects, numbers of pil ... | 1996 | 11363394 |
| azt and 3tc. | 3tc can raise t4 counts and lower hiv levels, and its use may increase the effectiveness of azt. studies show that hiv levels decrease by more than 90 percent in people taking the 2 drugs in combination. side effects are manageable, and few participants drop out of the studies. another study showed that 3tc also lowered the amount of hepatitis b virus in the blood to a level where it could not be detected. side effects include headache, nausea, fatigue, diarrhea, neuropathy, and lowered levels o ... | 1996 | 11363395 |
| indinavir (crixivan). | indinavir (crixivan) is one of the protease inhibitors approved for hiv treatment by the food and drug administration (fda). all studies to date have been limited to examining its effect on t4 cell changes and hiv viral load levels. current studies are looking at indinavir in combination with azt and 3tc. after sixteen weeks of combination therapy, viral loads in patients dropped to levels below those that can be measured with standard tests. however, these studies were done on small groups, and ... | 1996 | 11363398 |
| viral load testing. | currently, viral-load testing is still experimental, and while doctors can order tests, insurance companies may not be willing to pay for them. the two viral-load tests now available, the hoffmann-la roche pcr test and chiron's bdna test, are described in terms of how they measure viral load. concerns of these tests' accuracy and use of the test as a marker of disease progression are addressed. a study examining the viral load test is currently enrolling. decisions on hiv treatment will be made ... | 1996 | 11363399 |
| f-dda for hiv. | the national cancer institute (nci) is currently testing f-dda, related to ddi but easier to absorb. to qualify for the trial, patients must have 100-400 t4 counts and have been off all anti-hiv drugs for 3 weeks. interested patients are directed to call the aids treatment data network for a referral. | 1996 | 11363812 |
| hiv adult standard of care. act up. | the revised hiv adult standard of care guidelines, produced by act up/philadelphia, are printed. the extensive table summarizes the minimum standard of care needed for individuals with hiv infection to maximize their quality and length of life. divided into sections by t4 cell count, the table includes diagnostic tests and exams that patients may wish to have done at certain times in their disease progression. treatment options are suggested for hiv disease, opportunistic infections, and other a ... | 1996 | 11363824 |
| [the fourth retroviruses conference: good news with precautions]. | the fourth conference on retroviruses and opportunistic infections was held in washington, d.c. in january 1997. topics that were presented at the conference include reduced aids mortalities in new york, reduced hospitalizations for opportunistic infections, clinical advances in antiretroviral therapies, and treatment and prevention of opportunistic infections. drug therapies, viral load change, t4 cell counts, and peripheral neurodegeneration are discussed. special attention is given to combina ... | 1997 | 11364489 |
| saquinavir update. | actg 333 tested the effectiveness of other protease inhibitors in patients who had been on saquinavir for two years. early data showed that neither indinavir (crixivan) nor the new version of saquinavir known as the soft gel cap (sgc) had an effect on t4 cell counts or hiv levels. the people in the study only switched their protease inhibitor, not the other anti-hiv drugs that they were taking. as a result, new federal guidelines specify that all anti-hiv drugs should be changed when protease in ... | 1997 | 11364616 |
| expanded access programs. | sustiva (dmp 266 or efavirenz) has been available on an expanded access program since october 1. sustiva is a non-nucleoside reverse transcriptase inhibitor, has fewer side effects than other drugs of its class, and is taken once a day at bedtime. criteria for the program are t4 counts under 50 in patients whose current therapy is not working. adefovir dipivoxil (bis-pom pmea or preveon) is a nucleotide analog that is also available through an expanded access program. adefovir's properties make ... | 1997 | 11364931 |
| hiv treatment guidelines. | in response to the rapidly changing treatment options for hiv, the federal government issued draft guidelines for using hiv drugs in adults and adolescents. the main points of the new guidelines are described. no study has yet definitively determined the best time to start anti-hiv treatment, although the drugs have been shown to help people with aids live longer. people with aids or symptomatic hiv should begin treatment immediately, monitoring progress through viral load and t4 cell testing. v ... | 1997 | 11364934 |
| napwa sends thrush alert. national association of people with aids. | the national association of people with aids (napwa) is warning people that drug-resistant strains of oral candidiasis (thrush) can be transmitted. the group recommends that people with greater than 200 t4 cell counts discontinue prophylactic treatment, and that people with thrush receive treatment as needed to minimize the chance of developing resistant strains. | 1998 | 11364992 |
| understanding and managing resistance. | as many as 25 to 45 percent of patients using triple therapy with protease inhibitors will develop resistance due to a change in the genetic hiv code. however, patients who develop resistance may still benefit clinically when protease inhibitors are used in combination with other antiretrovirals. these patients may not have undetectable viral loads although they may have stable t4-cell counts. resistance does not always lead to disease progression. newer drugs under development or available thro ... | 1998 | 11364997 |
| latest opportunistic infection prevention guidelines. | although people with hiv are living longer than ever before, they continue to suffer from infections that are associated with low t4-cell counts. the u.s. public health service (phs) and the infectious diseases society of america (idsa) have updated the guidelines for preventing opportunistic infections, including their positions on when prophylaxis is indicated. summaries are given for preventing the following infections: cytomegalovirus (cmv), cryptosporidiosis, fungal infections, human papill ... | 1998 | 11365000 |
| hydroxyurea for hiv? | the anticancer drug, hydroxyurea, can help certain anti-hiv drugs work better. hydroxyurea's side effects, dosage requirements, and method of reducing hiv in the body are discussed. results from several small studies of hydroxyurea indicate that the addition of hydroxyurea to ddi produces a stronger anti-hiv effect than ddi alone. hydroxyurea may also help to increase t4 cell counts. it is uncertain how hydroxyurea combined with ddi will work in patients with low t4 cell counts and more advanced ... | 1998 | 11365419 |
| good news about hiv treatments. | new and stronger anti-hiv treatments are decreasing aids mortality and opportunistic infections statistics. studies in baltimore, new orleans, and france showed significant drops in new cases of cytomegalovirus (cmv), mycobacterium avium complex (mac), kaposi's sarcoma, and pcp. drug side effects and drug adherence remain problematic. it appears that even a short-term reduction in viral load can produce long-term improvement in health and increase t4 cell count. researchers speculate that hiv is ... | 1998 | 11365424 |
| taking anti-hiv drugs. | thirteen anti-hiv drugs are currently available by prescription, and most treatment regimens usually consist of a three- or four-drug combination called highly active antiretroviral therapy (haart). haart can be very effective in people who have aids or symptoms of hiv infection. however, for people who feel well and are in the earlier stages of infection, the best time to begin treatment is unclear. side effects of the drugs can be so unpleasant that quality of life is impacted. if the risk of ... | 1999 | 11366273 |
| immune control. | the immune system's job is protection from illness. following a long period of hiv infection, the immune system often fails. opportunistic infections may ensue during this slow process of disease progression. some hiv-positive people, however, do not experience immune system damage, do not develop opportunistic infections, and have undetectable levels of hiv. these long-term nonprogressors are being studied by dr. bruce walker from harvard university. background information on how the immune sys ... | 1999 | 11366299 |
| [effect of wulong dan on treatment of acute cerebral infarction and change of hormones in hypothalamus-pituitary-thyroid axis]. | to explore the effect of wulong dan (wld) on treatment of acute cerebral infarction and change of hormones in hypothalamus-pituitary-thyroid axis. | 1998 | 11367657 |
| thyroid function of former opioid addicts on naltrexone treatment. | in order to assess thyroid function in former opioid addicts undergoing adjunctive naltrexone (na) p.o. treatment, we studied 24 subjects (bmi +/- sd: 23.3 +/- 3.2 kg/m2) on 50 mg na p.o. daily for 15 days to 14.5 months continuously. measurements included thyrotropin (tsh), total thyroxin (tt4), total triiodothyronine (tt3), while the tt3/tt4100 ratio was calculated as a marker of peripheral conversion of t4 to t3. reverse t3 (rt3) and serum interleukin-6 (il-6) levels were also measured. stati ... | 2001 | 11367890 |
| breast cancer occurred after treatment for hodgkin's disease: analysis of 133 cases. | to assess the clinical and histological characteristics of breast cancer (bc) occurring after hodgkin's disease (hd) and give possible therapies and prevention methods. | 2001 | 11369065 |
| [efficacy of elective lymph node dissection in malignant melanoma of the extremities and trunk. an analysis in view of the sentinel lymph node biopsy]. | elective lymph node dissection (elnd) is increasingly displaced by the sentinel-lymph node biopsy. in this view we analyzed the efficacy of elnd in the treatment of malignant melanoma of the extremities and the trunc. between 1979 and 1998 we performed a lymph node dissection in 834 patients (336 male, 498 female; average age 52 years). the analysis of the prospectively collected data was based on those 650 patients in whom an elnd was performed for uicc-stage i (t2) to stage iii melanoma. the 5 ... | 2001 | 11370389 |
| [anastomosis protection and preservation of continence in the surgical treatment of rectal carcinoma]. | to find clues to a risk-adjusted therapy with regard to the use of protective colostomies and the value of hartmann-resection. | 2001 | 11370394 |
| impairment in the pituitary-thyroid axis of the camelus dromedarius infected with trypanosoma evansi. | camels infected with trypanosoma evansi were diagnosed by the passive haemagglutination test and ag-elisa and subjected to hormonal blood analyses to estimate the function of the thyroid gland. the results indicated that t. evansi caused a significant case of hypothyroidism evidenced as a decrease in the thyroid triiodothyronine (t3) and thyroxine (t4) blood levels associated with a parallel decrease in the level of blood pituitary thyrotropic hormone (tsh) as measured by the ria technique. | 2001 | 11370479 |
| [change in kidney function in elderly patients in the perioperative phase]. | reduction in organ function is a common problem in the perioperative period of the elderly patient. especially renal function is often decreased in this situation. | 2001 | 11370601 |
| increased serum level of sialyl lewis(x) antigen in blood from the tumor drainage vein in patients with non-polypoid growth type of colorectal cancer. | two types of colorectal cancer with distinct morphologies have been described in recent studies: polypoid growth type (pg-type) and non-polypoid growth type (npg-type). we hypothesize that the morphologic differences may correspond to additional biological distinctions. ratios of sialyl lewisa (ca 19-9), sialyl lewisx (slx), or carcinoembryonic antigen (cea) in the venous blood drainage from the tumor to that of the respective antigen in the peripheral venous blood (d/p ratio) was examined in or ... | 2001 | 11370835 |
| [standards, options and recommendations for the management of locally advanced non small cell lung carcinoma]. | the "standards, options and recommendations" (sor) project, started in 1993, is a collaboration between the federation of the french cancer centres (fnclcc), the 20 french cancer centres and specialists from french public universities, general hospitals and private clinics. the main objective is the development of clinical practice guidelines to improve the quality of health care and outcome for cancer patients. the methodology is based on literature review and critical appraisal by a multidisci ... | 2001 | 11371371 |
| disappearance of the negative charge in giant dna with a folding transition. | in the present study we measure the electrophoretic mobility of giant t4 dna (166 kbp) by electrophoretic light scattering for the elongated and folded compact states at different spermidine (trivalent cation) concentrations in 50 mm sodium maleate buffer (ph 6.0). it is found that the electrophoretic mobility of elongated dna in the absence of the multivalent cation is seven times greater than that of fully folded compact dna, where, with the increase of the concentration of spermidine, an abru ... | 2001 | 11371456 |
| results of salvage cryoablation of the prostate after radiation: identifying predictors of treatment failure and complications. | we conduct a critical evaluation of cryoablation of prostate cancer after failure of full dose radiotherapy to identify predictors of treatment failure and complications. | 2001 | 11371885 |
| thymine-metal ion interactions: relevance for thymine quartet structures. | apart from their function as counter ions for the charge neutralization of nucleic acids, alkali metal ions play important roles in stabilizing particular multistranded nucleic acids, e.g. guanine quartets in telomeres and uracil (u) or thymine (t) quartets. here x-ray crystal structure determinations of a series of alkali metal ions (na+, k+, rb+, cs+) as well as of mg2+ and h5o2+ adducts with the model bases 1-methylthymine and 1-ethylthymine are reported, which bear relevance to the question ... | 2001 | 11372196 |
| [a study on membrane fluidity of thyroid gland in rats with iodine deficiency or iodine excess]. | to explore the effect of iodine deficiency and excess on thyroid membrane fluidity and its pathogenesis. | 2000 | 11372391 |
| phenotypic and genotypic characterization of antagonistic bacteria associated with roots of transgenic and non-transgenic potato plants. | rhizobacteria obtained during a risk assessment study from parental and transgenic t4 lysozyme-expressing potato plants were investigated to determine whether or not the strains could be grouped based on the source of isolation, transgenic or non-transgenic plants, respectively. a total of 68 representative bacterial strains of the group of enterics and pseudomonads were investigated by phenotypic profiling (the antagonistic activity towards bacterial and fungal plant pathogens, the production o ... | 2001 | 11372657 |
| injection of thyrotrophin-releasing hormone in turkey embryos elevates plasma thyroxine concentrations. | the effectiveness of thyrotrophin-releasing-hormone (trh) as a secretagogue in turkey embryos was tested. fertilized turkey eggs were injected with trh after 24 d of incubation. in an experiment to determine an effective route and dose for trh administration, it was shown that a single manual injection of 200 microl containing 2.15 microg of trh, into the air cell or the same injection containing 5.0 microg through the bottom of the egg, was effective in elevating plasma concentrations of thyrox ... | 2001 | 11372716 |
| the course of anxiety, depression and drinking behaviours after completed detoxification in alcoholics with and without comorbid anxiety and depressive disorders. | we studied the associations between comorbid anxiety and depressive disorders in treated alcoholics, the course of current anxiety and depression during the early and late post-detoxification periods, and drinking behaviours after discharge. lifetime psychiatric comorbidity was assessed in 100 alcoholics using the composite international diagnostic interview (cidi). three subgroups defined as group da (comorbid depressive and anxiety disorders, n = 15), group a (anxiety disorder only, n = 23), a ... | 2001 | 11373263 |
| differential cell adhesion molecule expression and lymphocyte mobilisation during prolonged aerobic exercise. | this study was undertaken to determine the cell adhesion molecule profile of cd4+, cd8hi and cd56+ lymphocytes, which are mobilised to and from the peripheral blood during and after prolonged aerobic exercise. ten healthy males (21-35 years old) were tested on two occasions, separated by at least 14 days. on the first occasion, subjects were examined in a rested state but did not exercise. on the second occasion, the same subjects were examined at the same time of day before, during and after 2 ... | 2001 | 11374110 |
| bacteriophage t4 rnase h removes both rna primers and adjacent dna from the 5' end of lagging strand fragments. | bacteriophage t4 rnase h belongs to a family of prokaryotic and eukaryotic nucleases that remove rna primers from lagging strand fragments during dna replication. each enzyme has a flap endonuclease activity, cutting at or near the junction between single- and double-stranded dna, and a 5'- to 3'-exonuclease, degrading both rna.dna and dna.dna duplexes. on model substrates for lagging strand synthesis, t4 rnase h functions as an exonuclease removing short oligonucleotides, rather than as an endo ... | 2001 | 11376000 |
| a dual role for substrate s-adenosyl-l-methionine in the methylation reaction with bacteriophage t4 dam dna-[n6-adenine]-methyltransferase. | the fluorescence of 2-aminopurine ((2)a)-substituted duplexes (contained in the gatc target site) was investigated by titration with t4 dam dna-(n6-adenine)-methyltransferase. with an unmethylated target ((2)a/a duplex) or its methylated derivative ((2)a/(m)a duplex), t4 dam produced up to a 50-fold increase in fluorescence, consistent with (2)a being flipped out of the dna helix. though neither s-adenosyl-l-homocysteine nor sinefungin had any significant effect, addition of substrate s-adenosyl ... | 2001 | 11376154 |
| immunolocalization of the na(+)-k(+)-2cl(-) cotransporter in peripheral nervous tissue of vertebrates. | efflux of cl(-) through gaba(a)-gated anion channels depolarizes the cell bodies and intraspinal terminals of sensory neurons, and contributes to the generation of presynaptic inhibition in the spinal cord. active accumulation of cl(-) inside sensory neurons occurs through an na(+)-k(+)-2cl(-) cotransport system that generates and maintains the electrochemical gradient for this outward cl(-) current. we studied the immunolocalization of the na(+)-k(+)-2cl(-) cotransporter protein using a monoclo ... | 2001 | 11377856 |
| influence of prophylactic use of pentoxifylline on postoperative organ function in elderly cardiac surgery patients. | to study the effects of pretreatment with pentoxifylline before cardiac surgery on postoperative organ function in elderly patients (>80 yrs) undergoing cardiac surgery. | 2001 | 11378603 |
| the 1989 patterns of care study for prostate cancer: five-year outcomes. | five-year results from the 1989 patterns of care study (pcs) for prostate cancer are now ready for analysis. the pcs was initiated to determine national averages for treatments and examine outcomes prospectively; the 1989 prostate study is the first to have collected pre- and post-treatment serum psa data. | 2001 | 11380218 |
| squamous cell carcinomas of the soft palate treated with radiation therapy alone or followed by planned neck dissection. | the present study presents the experience at the university of florida with treatment of unselected patients with carcinomas of the soft palate with radiation therapy (rt) alone or followed by planned neck dissection. | 2001 | 11380222 |
| self-splicing of the tetrahymena group i ribozyme without conserved base-triples. | group i introns share a conserved core region consisting of two domains, p8-p3-p7 and p4-p6, joined by four base-triples. we showed previously that the t4 td intron can perform phosphoester transfer reactions at two splice sites in the absence of both p4-p6 and the conserved base-triples, whereas it is barely able to perform the intact splicing reaction due to the difficulty of conducting the sequential reactions. | 2001 | 11380619 |
| [primary carcinoma of the ear canal. clinical aspects, intratemporal growth behavior and surgical strategy]. | the symptoms and microscopic findings in carcinoma of the external auditory meatus are often unspecific and misinterpreted as recurrent otitis externa. a definite diagnosis is therefore often delayed, frequently resulting in advanced tumor stages with unfavorable prognosis at the time of diagnosis. | 2001 | 11382105 |
| [sporadic primary hypothyroidism in children yesterday and today]. | the evolution of congenital primary hypothyroidism has extremely capitalized on early l-t4 substitutive therapy, as a result of neonatal screening techniques, that allow early diagnosis. however, iq and neurodevelopment of patients diagnosed and treated in this manner are yet quite different from those normal controls. latest efforts to achieve precocious neonatal diagnosis have led to earlier initiation of treatments. these circumstances, in addition to the use of higher l-t4 doses (between 10 ... | 2000 | 11382160 |
| fidelity of enzymatic ligation for dna computing. | we describe a convenient assay for rapid qualitative evaluation of hybridization/ligation fidelity. the approach uses randomized probe strands of dna and restriction enzyme digestion after amplification of reaction products by the polymerase chain reaction (pcr). we report ligation efficiencies and fidelities of two dna ligases, t4 dna ligase and thermus aquaticus (taq) dna ligase, over a range of temperatures. | 2000 | 11382365 |
| pullout strength of pedicle screws versus pedicle and laminar hooks in the thoracic spine. | while the biomechanical properties of pedicle screws have proven to be superior in the lumbar spine, little is known concerning pullout strength of pedicle screws in comparison to hooks in the thoracic spine. in vitro biomechanical pullout testing was performed to evaluate the axial pullout strength of pedicle screws versus pedicle and laminar hooks in the thoracic spine with regard to surgical correction techniques in scoliosis. nine human cadaveric thoracic spines were harvested and disarticul ... | 2001 | 11383294 |
| prophylactic use of pentoxifylline on inflammation in elderly cardiac surgery patients. | inflammation plays a pivotal role in the pathogenesis of organ injury after cardiopulmonary bypass (cpb). elderly patients appear to be especially prone to develop general inflammation. use of pentoxifylline (ptx) before surgery may be a promising approach to minimize the negative effects of cpb in these patients. | 2001 | 11383794 |
| effects of thyroid hormones on bovine granulosa and thecal cell function in vitro: dependence on insulin and gonadotropins. | the objective of this study was to evaluate the influence of thyroxine (t4) and triiodothryonine (t3) on steroid production by bovine granulosa and thecal cells. granulosa and thecal cells were obtained from small (1 to 5 mm) and large (> or = 8 mm) follicles of cattle, respectively, and cultured for 4 d. we conducted six experiments to evaluate the effect of 2 d of exposure to various doses of t3 or t4. in insulin- or insulin plus fsh-treated granulosa cells of experiment 1, 30 and 100 ng/ml of ... | 2001 | 11384033 |
| nmp 22, bta stat test and cytology in the diagnosis of bladder cancer: a comparative study. | to evaluate the usefulness of the nmp 22 and bta stat test in the diagnosis and follow-up of bladder cancer and to compare these tests to cytology and cystoscopy, routine diagnostic methods. | 2001 | 11385303 |
| [effect of cultivation conditions on the functional activity of thyrocytes in the organ culture of the thyroid gland in newborn piglets]. | in new-born piglets' thyroid gland culture, thyrocytes were capable of synthesising thyroxine and triiodothyronine for over 12 days. a 48-hour decline of the medium ph to 6.45 did not affect further functional activity of the cells. decrease in temperature to 26-28 degrees c augmented the thyroid hormones level as opposed to continuous cultivation at 37 degrees c. degradation of the thyroid gland follicular structure induced a change in the hormones biosynthesis. the thyrocytes not arranged in t ... | 2001 | 11386162 |
| programmed escherichia coli cell lysis by expression of cloned t4 phage lysis genes. | self-disruptive escherichia coli that produces foreign target protein was developed. e. coli was co-transformed with two vector plasmids, a target gene expression vector and a lysis gene expression vector. the lytic protein was produced after the expression of the target gene, resulting in simplification of the cell disruption process. in this study, the expression of cloned t4 phage gene e or t was used for the disruption of e. coli that produced beta-glucuronidase (gus) as a model target prote ... | 2001 | 11386882 |
| development of a phantom for morphometric x-ray absorptiometry. | morphometric x-ray absorptiometry (mxa) has recently been developed to assess vertebral deformity status using dual energy x-ray absorptiometry (dxa) machines. in contrast to bone densitometry, a vertebral morphometry phantom is not supplied by any machine manufacturer. the aim of this study was to develop a suitable phantom to quantify the accuracy and precision of the vertebral measurement software on three dxa scanners in vitro and to perform a weekly quality control (qc) scan over a 30-month ... | 2001 | 11387153 |
| myelination of prospective large fibres in chicken ventral funiculus. | in mammals, the oligodendrocyte population includes morphological and molecular varieties. we reported previously that an antiserum against the t4-o molecule labels a subgroup of oligodendrocytes related to large myelinated axons in adult chicken white matter. we also reported that t4-o immunoreactive cells first appear in the developing ventral funiculus (vf) at embryonic day (e)15, subsequently increasing rapidly in number. relevant fine structural data for comparison are not available in the ... | 2000 | 11387549 |
| [relationship between expression of p16 protein and prognosis in carcinoma of nasopharynx]. | we observed the relationship between p16 gene and the occurrence, development and prognosis of carcinoma of nasopharynx by means of lsab immunohistochemistry method and investigated the expression of protein p16 in 73 cases of carcinoma of nasopharynx and 10 cases of epithelia of chronic inflammation of nasopharynx. the results showed that p16 positive rate was 100% for the epithelia of chronic inflammation of nasopharynx. it was significantly higher than the p16 positive rate for the carcinoma ... | 1999 | 11387949 |
| prognostic value of p53 mutations in patients with locally advanced esophageal carcinoma treated with definitive chemoradiotherapy. | a significant correlation has been found between p53 mutation and response to chemotherapy or radiotherapy. to determine the prognostic value of p53 mutation in patients with locally advanced esophageal carcinoma treated with definitive chemoradiotherapy, p53 mutation was analyzed using the biopsied specimens taken for diagnosis. | 2001 | 11388392 |
| mechanical testing of a single rod versus a double rod in a long-segment animal model. | this study involved the mechanical testing of single-rod segmental hook fixation and double-rod segmental hook fixation in a long-segment animal model. the goals were first to compare the flexibility of a single-rod scoliosis construct with that of a double-rod construct when tested in torsion, and second, to determine the effect of not using instrumentation with every vertebral segment for the single rod. another study found that the single-rod construct was as stiff in torsion as the standard ... | 2001 | 11389374 |
| conditional coupling of leading-strand and lagging-strand dna synthesis at bacteriophage t4 replication forks. | eight proteins encoded by bacteriophage t4 are required for the replicative synthesis of the leading and lagging strands of t4 dna. we show here that active t4 replication forks, which catalyze the coordinated synthesis of leading and lagging strands, remain stable in the face of dilution provided that the gp44/62 clamp loader, the gp45 sliding clamp, and the gp32 ssdna-binding protein are present at sufficient levels after dilution. if any of these accessory proteins is omitted from the dilutio ... | 2001 | 11390383 |
| changes in synaptic inputs to sympathetic preganglionic neurons after spinal cord injury. | spinal cord injury (sci) leads to plastic changes in organization that impact significantly on central nervous control of arterial pressure. sci causes hypotension and autonomic dysreflexia, an episodic hypertension induced by spinal reflexes. sympathetic preganglionic neurons (spns) respond to sci by retracting and then regrowing their dendrites within 2 weeks of injury. we examined changes in synaptic input to spns during this time by comparing the density and amino acid content of synaptic in ... | 2001 | 11391643 |
| [thyroid function tests]. | about 80% of thyroid disease consists of thyroid-specific autoimmune diseases, hashimoto's disease and grave's disease. to diagnose thyroid diseases, testings for (1) thyroid function and (2) pathogenetic autoantibodies are indispensable. to assess thyroid function, serum hormone concentrations, such as tsh, ft4 and ft3 are measured. among these hormones, serum tsh concentrations are the most reliable and informative regarding thyroid function, correcting indicating a hyperthyroid, euthyroid or ... | 2001 | 11391943 |
| effects of continuous and intermittent exposure of lactating mothers to aroclor 1242 on testicular steroidogenic function in the adult male offspring. | polychlorinated biphenyls (pcbs) are worldwide pollutants and have caused hazardous effects on many animal species including humans. they have been detected in human milk and therefore exposure of newborns to pcbs is unavoidable if they are breast-fed. we present our findings on two experiments performed to test the effects of intermittent and continuous exposure of lactating rats to two different doses (80 microg and 8 microg) of aroclor 1242 (a pcb congener) on testicular steroidogenic functio ... | 2001 | 11392670 |
| examining prognostic factors and patterns of failure in nasopharyngeal carcinoma following concomitant radiotherapy and chemotherapy: impact on future clinical trials. | concomitant chemotherapy and radiotherapy (ccrt), followed by adjuvant chemotherapy, has improved the outcome of nasopharyngeal carcinoma (npc). however, the prognosis and patterns of failure after this combined-modality treatment are not yet clear. in this report, the prognostic factors and failure patterns we observed with ccrt may shed new light in the design of future trials. | 2001 | 11395240 |
| replisome-mediated dna replication. | the elaborate process of genomic replication requires a large collection of proteins properly assembled at a dna replication fork. several decades of research on the bacterium escherichia coli and its bacteriophages t4 and t7 have defined the roles of many proteins central to dna replication. these three different prokaryotic replication systems use the same fundamental components for synthesis at a moving dna replication fork even though the number and nature of some individual proteins are dif ... | 2001 | 11395406 |
| reduced levels of thyroid hormones, insulin, and glucose, and lower body core temperature in the growth hormone receptor/binding protein knockout mouse. | the mechanisms that are responsible for the extension of lifespan in the mouse with targeted disruption (knockout [ko]) of the growth hormone (gh) receptor/binding protein (ghr-ko) are unknown. however, in the long-living ames dwarf mouse, blood glucose and body core temperature (tco) are consistently lower than in normal mice. in addition, insulin levels are reduced and corticosterone levels are elevated in male dwarfs. these functional alterations, similar to those seen in animals under calori ... | 2001 | 11395925 |
| update on intrathyroidal iodine metabolism. | the thyroid concentrates iodide from the serum and oxidizes it at the apical membrane, attaching it to tyrosyl residues within thyroglobulin (tg) to make diiodotyrosine and monoiodotyrosine. major players in this process are tg, thyroperoxidase (tpo), hydrogen peroxide, pendrin, and nicotinamide adenine dinucleotide phosphate (nadph). further action of tpo, hydrogen peroxide (h2o2), and iodinated tg produce thyroxine (t4) and triiodothyronine (t3). hormone-containing tg is stored in the follicul ... | 2001 | 11396699 |
| pregnancy and iodine. | hormonal changes and metabolic demands during pregnancy result in profound alterations in the biochemical parameters of thyroid function. for thyroid economy, the main events occurring during pregnancy are a marked increase in serum thyroxine-binding globulin levels; a marginal decrease in free hormone concentrations (in iodine-sufficient areas) that is significantly amplified when there is iodine restriction or overt iodine deficiency; a frequent trend toward a slight rise in basal thyrotropin ... | 2001 | 11396705 |
| iodine-induced hypothyroidism. | iodine is an essential element for thyroid hormone synthesis. the thyroid gland has the capacity and holds the machinery to handle the iodine efficiently when the availability of iodine becomes scarce, as well as when iodine is available in excessive quantities. the latter situation is handled by the thyroid by acutely inhibiting the organification of iodine, the so-called acute wolff-chaikoff effect, by a mechanism not well understood 52 years after the original description. it is proposed that ... | 2001 | 11396709 |
| uveodermatological syndrome (vogt-koyanagi-harada-like syndrome) with generalized depigmentation in a dachshund. | a 3-year-old, female, black and tan dachshund was referred with visual impairment, bilateral anterior and posterior uveitis, poliosis, and generalized dermal depigmentation. complete blood counts and biochemical parameters, including t3 and t4, were normal. the skin biopsy showed lichenoid dermatoses with dermal infiltration of histiocytes and lymphocytes suggesting uveodermatological syndrome. medical treatment was initiated with oral prednisone and azathioprine, and topical prednisolone and at ... | 1998 | 11397209 |
| intracavitary afterloading boost in anal canal carcinoma. results, function and quality of life. | first clinical data on a new intracavitary afterloading boost method for anal canal carcinoma is reported. | 2001 | 11398611 |
| dissection of the atp-driven reaction cycle of the bacteriophage t4 dna replication processivity clamp loading system. | processive dna replication requires the loading of a multisubunit ring-shaped protein complex, known as a sliding or processivity clamp, onto the primer-template (p/t) dna. this clamp then binds to the replication polymerase to form a processive polymerase holoenzyme. the processivity of the holoenzyme derives from the topological properties of the clamp, which encircles the dna without actually binding to it. multisubunit complexes known as clamp-loaders utilize atp to drive the placement of th ... | 2001 | 11399065 |
| analysis of the roles of trna structure, ribosomal protein l9, and the bacteriophage t4 gene 60 bypassing signals during ribosome slippage on mrna. | a 50-nucleotide coding gap divides bacteriophage t4 gene 60 into two open reading frames. in response to cis-acting stimulatory signals encrypted in the mrna, the anticodon of the ribosome-bound peptidyl trna dissociates from a gga codon at the end of the first open reading frame and pairs with a gga codon 47 nucleotides downstream just before the second open reading frame. mutations affecting ribosomal protein l9 or trna(gly)(2), the trna that decodes gga, alter the efficiency of bypassing. to ... | 2001 | 11399077 |
| entry of thyroid hormones into tilapia oocytes. | the patterns of entry of thyroid hormones into live tilapia oocytes were examined by incubating ovarian follicles in l-15 medium containing 125i-labeled thyroxine (t4) or 3,5,3'-triiodothyronine (t3). as judged from hplc profiles, radioactivity in extracts of follicles immersed in t3 was identified to reside in t3, while most of the radioactivity in the extract of t4 immersed follicle was not associated with t4. radioactivity of t3 immersed follicles reached a constant level after 18 h of incuba ... | 2001 | 11399496 |
| the significance of comorbidity in advanced laryngeal cancer. | cancer patients often have concurrent diseases and conditions known as comorbidities. the aim of this project is to demonstrate the significance of comorbidity in the treatment and outcomes of advanced laryngeal carcinoma. | 2001 | 11400245 |
| discrimination of anesthetic states using mid-latency auditory evoked potential and artificial neural networks. | this study was undertaken to determine whether artificial neural network (ann) processing of mid-latency auditory evoked potentials (mlaeps) can identify different anesthetic states during propofol anesthesia, and to determine those parameters that are most useful in the identification process. twenty-one patients undergoing elective abdominal surgery were studied. to maintain general anesthesia, the patients received propofol (3-5 mgkg(-1) h(-1) intravenously). epidural analgesia at the level o ... | 2001 | 11400725 |
| neonatal screening for hypothyroidism at a university hospital in thailand. | neonatal screening is an essential program for early identification of congenital hypothyroidism. between july 1991 and may 1998, 37, 262 infants born at chulalongkorn hospital were enrolled to the screening program. blood tsh levels were determined on infants at > or = 48 hours after birth. they were performed in dried blood sample taken by heel prick on filter papers. tsh levels were measured by fluoroimmunoassay from july 1991 to december 1997 and by immunoradiometric assay from january to ma ... | 1999 | 11400772 |
| newborn screening for pku and congenital hypothyroidism in latvia. | the newborn screening program in latvia was started in 1980 as pilot study project. mass screening for phenylketonuria (pku) in the whole republic was started in 1987, but for congenital hypothyroidism (ch) it begun in 1996. last two years the latvian state medical genetics center (smgc) screened nearly 25,000 newborn dried blood specimens per year. approximately 25% are repeat and control specimens. efficiency of the screening program is nearly 97%. fluorometric method is used for pku screening ... | 1999 | 11400783 |
| dynamics of translesion dna synthesis catalyzed by the bacteriophage t4 exonuclease-deficient dna polymerase. | the mechanism and dynamics of translesion dna synthesis were evaluated using primer/templates containing a tetrahydrofuran moiety designed to mimic an abasic site. steady-state kinetic analysis reveals that the t4 dna polymerase preferentially incorporates datp across from the abasic site with 100-fold higher efficiency than the other nucleoside triphosphates. under steady-state conditions, the catalytic efficiency of datp incorporation across from an abasic site is only 220-fold lower than that ... | 2001 | 11401565 |