Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| triiodothyronine stimulates the release of membrane-bound alkaline phosphatase in osteoblastic cells. | thyroid hormone deficient osteoblastic cells in cell culture released a significantly higher amount of alkaline phosphate (alp) activity following t3 replacement. t3 increased the release of total and membrane-bound alp activity in these cells significantly more than t4 or inactive thyroid hormone metabolite, dit. the effect of t3 on the membrane-bound alp fraction was dose and time dependent; higher concentrations of t3 and longer incubation time with t3 proportionally increased the enzyme acti ... | 2000 | 11289695 |
| influence of propylthiouracil treatment on oxidative stress and nitric oxide in basedow disease patients. | oxidative stress parameters and nitric oxide (no) values were determined in 27 newly diagnosed basedow patients before and after 1 mo of propylthiouracil (ptu) therapy and in 15 healthy controls. basedow patients exhibited increased triiodothyronine (t3) and thyroxine (t4) and decreased thyroid-stimulating hormone (tsh) values compared to controls. significantly higher thiobarbituric acid-reactive substances (tbars), no and glutathione (gsh) levels, and cuzn superoxide dismutase (cuzn sod) activ ... | 2001 | 11289700 |
| the 3' --> 5' exonuclease of t4 dna polymerase removes premutagenic alkyl mispairs and contributes to futile cycling at o6-methylguanine lesions. | we have studied the processing of o(6)-methylguanine (m6g)-containing oligonucleotides and n-methyl-n-nitrosourea (mnu)-treated dna templates by the 3' --> 5' exonuclease of t4 dna polymerase. in vitro biochemical analyses demonstrate that the exonuclease can remove bases opposite a defined m6g lesion. the efficiency of excision of a terminal m6g.t was similar to that of m6g.c, and both were excised as efficiently as a g.t substrate. partitioning assays between the polymerase and exonuclease act ... | 2001 | 11290737 |
| radiation therapy (rt) after prostatectomy: the case for salvage therapy as opposed to adjuvant therapy. | patients with pathologic stage t3 or t4 prostate cancer who have undetectable psa levels following radical retropubic prostatectomy (rrp) have a substantial risk of recurrence. radiotherapy (rt) can be administered immediately following the rrp (immediate adjuvant rt) or may be postponed until the psa level has risen to a level that is indicative of residual or recurrent prostate cancer (salvage rt). immediate adjuvant rt can significantly reduce the risk of relapse, but does not appear to incre ... | 2001 | 11291092 |
| circulating sialyl lewis(x), sialyl lewis(a), and sialyl tn antigens in colorectal cancer patients: multivariate analysis of predictive factors for serum antigen levels. | preoperative serum levels of sialyl lewis(a) (ca 19-9), sialyl lewis(x) (slx), and sialyl tn (stn) antigens in colorectal cancer patients were examined to establish predictive factors for serum levels of these antigens compared with carcinoembryonic antigen (cea). a total of 308 patients who underwent resection for a colorectal cancer were divided into low and high antigen groups (higher or lower than a selected diagnostic-based cutoff value). the cutoff values were 37 u/ml for ca19-9, 38 u/ml f ... | 2001 | 11291879 |
| subjective response to and tolerability of long-term supraphysiological doses of levothyroxine in refractory mood disorders. | although supplementation with supraphysiological doses of levothyroxine (t4) has been an effective treatment for refractory affective disorders in open studies, questions remain as to the tolerability of this treatment. this is the first study to investigate subjective patient response and tolerability to long-term treatment with adjunctive t4. | 2001 | 11292518 |
| euthyroid sick syndrome in children with hodgkin disease. | euthyroid sick syndrome is related to profound changes in thyroid metabolism induced by nonthyroidal diseases. to determine whether children with newly diagnosed hodgkin disease might present thyroid abnormalities and to establish their predictive value, the authors performed regular thyroid function testing. seven children (5 m, 2 f) with a mean age of 10.4 years (range: 4.6-15 years) at diagnosis were studied for a period of 6.9 years (4.2-10.5 years). five patients presented at diagnosis with ... | 2001 | 11293290 |
| anal carcinoma: prognostic value of endorectal ultrasound (erus). results of a prospective multicenter study. | the classification of anal carcinoma is based on the clinical examination and the estimation of the tumor height (union internationale contre le cancer (uicc) 1987 classification). this classification has a direct therapeutic application since tumors which are designated t1 and t2 are generally treated by radiotherapy whereas t3, t4 or n+ lesions are treated by concomitant radiation and chemotherapy. the aim of this prospective multicenter study was to evaluate endorectal ultrasound (erus) and t ... | 2001 | 11293755 |
| renal cell carcinoma with extension of tumor thrombus into the vena cava: surgical strategy and prognosis. | the outcome of patients who underwent radical resection of renal cell carcinoma extending into the vena cava was retrospectively analyzed, and risk factors for long-term survival were investigated. | 2001 | 11296333 |
| [a thyrotoxic myopathy accompanied with unusual muscle symptoms and mri muscle findings]. | we report a patient with thyrotoxic myopathy associated with unusual muscle symptoms. a 29-year-old man developed hyperhidrosis, diarrhea, increase in appetite, and excitability in july, 1999. in august, he experienced muscle stiffness in bilateral lower extremities after maintaining postures such as driving a car or sitting on a chair. he was admitted to our hospital, in january, 2000. on physical examination, goiter was noted. neurological examination was normal except for proximal muscle weak ... | 2001 | 11296402 |
| hypothalamic-pituitary-thyroidal axis dysfunction and cortisol secretion in patients with nonclassical congenital adrenal hyperplasia. | the purpose of this study was to evaluate thyroid function and tsh and cortisol (f) secretion in hyperandrogenemic women with nonclassical congenital adrenal hyperplasia (nc-cah) due to 21-hydroxylase deficiency (group a) when compared with women with hyperandrogenemic symptoms (menstrual irregularities, hirsutism, acne, seborrhea and sterility) of other etiologies (group b). | 2001 | 11296811 |
| are the parameters of various stabilization factors estimated from mutant human lysozymes compatible with other proteins? | the various factors which contribute to protein stability have been extensively examined using mutant proteins, but the same kinds of substitutions have given different results depending on the substitution sites. recently, the contributions of some stabilization factors have been quantitatively derived as parameters by a unique equation, considering the conformational changes due to the mutations using mutant human lysozymes [funahashi et al. (1999) protein eng: 12, 841-850]. to evaluate these ... | 2001 | 11297670 |
| congenital thyrotoxicosis in premature infants. | graves' disease (gd) complicates 0.1% to 0.2% of pregnancies, but congenital thyrotoxicosis is rare occurring in one in 70 of these pregnancies independent of maternal disease status. antenatal prediction of affected infants is imprecise; however, maternal history, coupled with a high maternal serum tsh receptor binding immunoglobulin index (tbii) predict adverse neonatal outcome. mortality is reported to be as high as 25% in affected infants and would therefore be expected to be higher in prema ... | 2001 | 11298090 |
| efficacy of single daily dosage of methimazole vs. propylthiouracil in the induction of euthyroidism. | previous studies of the treatment of hyperthyroidism with a single daily dose of antithyroid drugs have demonstrated a favourable result with methimazole (mmi). however, the efficacy of a single daily dose of propylthiouracil (ptu) was inconsistent. the present prospective randomized study was conducted to compare the efficacy of a single daily dose of mmi and ptu in the induction of euthyroidism in patients with graves' disease. | 2001 | 11298092 |
| positional change of the hyoid bone after bilateral sagittal split osteotomy with rigid and wire fixation. | the purpose of this study was to compare positional changes of the hyoid bone and the amount of postsurgical compensation in mandibular position in patients who received either wire or rigid fixation after surgery. data were analyzed from 97 patients (25 males and 72 females) who were randomized to receive wire (43) or rigid (54) fixation after mandibular advancement surgery as part of a multicenter clinical trial. radiographs were digitized before surgery (t2), immediately after surgery (t3), a ... | 2001 | 11298311 |
| thyrotropin-releasing hormone stimulation test to assess thyroid function in severely sick cats. | basal serum thyroxine (t4) concentration and the thyrotropin-releasing hormone (trh) stimulation test were used to assess thyroid function in 36 critically ill cats examined between july 1996 and october 1998. of the 36 cats. hyperthyroidism (as underlying or complicating disease) was suspected in 22 based on clinical signs, palpable thyroid nodules, and abnormal thyroid gland histology (study group). hyperthyroidism was not suspected in the remaining 14 cats, which served as the control group. ... | 2001 | 11300603 |
| molecular motion of spin labeled side chains in alpha-helices: analysis by variation of side chain structure. | two single cysteine substitution mutants at helix surface sites in t4 lysozyme (d72c and v131c) have been modified with a series of nitroxide methanethiosulfonate reagents to investigate the structural and dynamical origins of their electron paramagnetic resonance spectra. the novel reagents include 4-substituted derivatives of either the pyrroline or pyrrolidine series of nitroxides. the spectral line shapes were analyzed as a function of side chain structure and temperature using a simulation ... | 2001 | 11300763 |
| hydroxypropyl-beta-cyclodextrin as delivery system for thyroid hormones, regulating glutathione s-transferase expression in rat hepatocyte co-cultures. | thyroid hormones play a role in the regulation of glutathione s-transferase (gst) expression. here, co-cultures of rat hepatocytes with bile duct epithelial cells have been used to study the direct effects of both triiodothyronine (t3) and thyroxine (t4) on gst activities and proteins. because t3 and t4 are poorly water soluble and organic solvents used to dissolve them often interfere with biotransformation pathways, an alternative delivery system namely hydroxypropyl-beta-cyclodextrin (hpbc) h ... | 2001 | 11301040 |
| duration of symptoms prior to diagnosis is related inversely to presenting disease stage in children with medulloblastoma. | the authors tested the hypothesis that children with a longer duration of symptoms prior to diagnosis of medulloblastoma have more advanced disease. in addition, they evaluated whether there are correlations between gender, duration of presenting symptoms, and disease stage. | 2001 | 11301391 |
| vertebral morphometry: a comparison of long-term precision of morphometric x-ray absorptiometry and morphometric radiography in normal and osteoporotic subjects. | vertebral morphometry, the quantification of vertebral body shape, has proved a useful tool in the identification and evaluation of osteoporotic vertebral deformities in both epidemiologic surveys and clinical trials. although conventionally it has been performed on lateral radiographs of the thoracolumbar spine (morphometric radiography, mrx), it may now be accomplished on morphometric x-ray absorptiometry (mxa) scans, acquired on dual-energy x-ray absorptiometry (dxa) machines. in this study t ... | 2001 | 11303717 |
| comparative bioavailability of two oral l-thyroxine formulations after multiple dose administration in patients with hypothyroidism and its relation with therapeutic endpoints and dissolution profiles. | the aim of the present study was to evaluate the bioequivalence and therapeutic equivalence of the two most commonly prescribed l-thyroxine (monsodium l-thyroxine hydrate, cas 25416-65-3) formulations in brazil in patients treated for hypothyroidism. twenty-four patients received 100 micrograms l-thyroxine daily of either puran t4 (test) or the brazilian reference formulation (reference) during 42 days, in a two-period crossover design. serum samples obtained over a 24-h interval were analyzed f ... | 2001 | 11304941 |
| sex hormone-binding globulin in gestational diabetes. | insulin is an important regulator of serum sex hormone-binding globulin (shbg) concentration which works by inhibiting its production in hepatocytes. low shbg level is associated with increased insulin resistance and hyperinsulinemia. our purpose was to compare maternal serum shbg level between normal and gestational diabetic pregnant women and to study the relationships between shbg, shbg/insulin and shbg/glucose ratio and several endocrine, metabolic and clinical parameters. | 2000 | 11304966 |
| [staging and neoadjuvant therapy of squamous cell carcinoma of esophagus]. | once the diagnosis of esophageal cancer is established, the decision on treatment will depend on the stage of the disease. since improvement of prognosis can only be expected in patients with complete removal of their tumor, preoperative staging plays a pivotal role in the decision-making process. preoperative diagnostic procedures should define the tumor in its relation to the tracheal bifurcation (site), determine the depth of tumor invasion (t status), evaluate regional lymph node metastases ... | 2001 | 11305155 |
| thyroid function and serum prolactin levels in patients with juvenile systemic lupus erythematosus. | to evaluate both thyroid function and serum prolactin levels in patients with juvenile systemic lupus erythematosus (jsle) and to detect possible correlation with disease activity. | 2001 | 11305794 |
| thyroid hormone profile in children with goiter in an endemic goiter area. | the thyroid hormone profile was investigated in goitrous schoolchildren aged 6-11 years living in antalya, an area with mild/ moderate iodine deficiency. with few exceptions, the serum levels of t4 and tsh were in the normal range in children with different grades of goiter. compensatory elevated t3 levels were detected in 24% of the subjects. thyroid hormones did not differ significantly with respect to the urinary iodine (ui) level. no correlations were found between thyroid volume, ui excreti ... | 2001 | 11305795 |
| molecular cloning of cdna encoding thyroid stimulating hormone beta subunit of bighead carp aristichthys nobilis and regulation of its gene expression. | the complementary dna (cdna) encoding pituitary thyroid stimulating hormone beta subunit (tsh-beta) of bighead carp was cloned and regulation of its gene expression was investigated for understanding phylogenetic divergence and evolution of tsh molecule. the cdna was obtained from bighead carp pituitary total rna by reverse transcription and polymerase chain reaction. oligonucleotide primers were designed from the sequence of common carp. the full length sequence was then obtained by 3' and 5' r ... | 2001 | 11306166 |
| excretion of matrix metalloproteinases 2 and 9 in urine is associated with a high stage and grade of bladder carcinoma. | to analyze the excretion of matrix metalloproteinases (mmps) 2 and 9 in the urine of patients with bladder cancer according to the stage and grade of tumor and to evaluate their diagnostic clinical validity. in numerous carcinomas, increased expression of mmps is associated with a higher grade of malignancy and poor prognosis. | 2001 | 11306379 |
| calgb 9380: a prospective trial of the feasibility of thoracoscopy/laparoscopy in staging esophageal cancer. | the staging of esophageal cancer is imprecise. thoracoscopic/laparoscopic (ts/ls) staging has been proposed as a more accurate lymph node (ln) staging method. we report the experience of an intergroup nci trial (calgb 9380) evaluating the feasibility and accuracy of this staging modality. | 2001 | 11308139 |
| haemodynamic changes and vasopressin release are not consistently associated with carbon dioxide pneumoperitoneum in humans. | conflicting haemodynamic changes, suggested to be caused by vasopressin release, have been reported during carbon dioxide (co2) pneumoperitoneum. however, peritoneal stimulations including open surgery cause both a systemic vasopressor response and a vasopressin release, which are suppressed by opiate administration. also, a decreased venous return of blood to the heart causes vasopressin release. furthermore, previous haemodynamic assessments of laparoscopic surgery have been conducted using va ... | 2001 | 11308999 |
| identification and mapping of protein-protein interactions between gp32 and gp59 by cross-linking. | the bacteriophage t4 59 protein (gp59) plays a vital role in recombination and replication by promoting the assembly of the gene 41 helicase (gp41) onto dna, thus enabling replication as well as strand exchange in recombination. loading of the helicase onto gp32 (the t4 single strand binding protein)-coated single-stranded dna requires gp59 to remove gp32 and replace it with gp41. cross-linking studies between gp32 and gp59 reveal an interaction between cys-166 of gp32 and cys-42 of gp59. since ... | 2001 | 11309384 |
| an optimized recipe for cloning of the polymerase chain reaction-amplified dna inserts into plasmid vectors. | this study compares a number of parameters that are important in the ligation of the polymerase chain reaction-amplified dna inserts into plasmid vectors and their efficient transformation to bacterial cells. the parameters covered were: t4 polynucleotide kinase treatment followed by either the large fragment of e. coli dna polymerase or t4 dna polymerase reactions, the amount of t4 dna ligase, temperature and duration of ligation, molar ratio of insert to vector as well as the total dna concent ... | 2000 | 11310983 |
| thyroxine signal transduction in liver cells involves phospholipase c and phospholipase d activation. genomic independent action of thyroid hormone. | numerous investigations demonstrate a novel role of thyroid hormone as a modulator of signal transduction. protein kinase c (pkc) is critical to the mechanism by which thyroid hormones potentiate both the antiviral and immunomodulatory actions of ifngamma in different cells and regulate the exchange of signalling phospholipids in hepatocytes. because nothing is known about accumulation of pkc modulator - diacylglycerol in cells treated with t4, we examined the nongenomic effect of thyroid hormon ... | 2001 | 11312999 |
| thyrotropin dysregulation during a non-thyroidal illness: transient hypothalamic hypothyroidism? | both the basal tsh concentration and the tsh response to iv trh administration are noted to be decreased at the peak of an acute critical illness. moreover, an impaired release from hypothalamus has been documented in rats with uncontrolled diabetes, suggesting hypothalamic dysfunction in a non-thyroidal illness. however, the exact inference and mechanism of this impaired tsh secretary pattern is not well defined in humans during a non-thyroidal illness. therefore, this study assessed hypothalam ... | 2001 | 11314747 |
| iodine speciation in human serum by reversed-phase liquid chromatography-icp-mass spectrometry. | reversed-phase liquid chromatography-inductively coupled plasma mass spectrometric hyphenation was used for iodine speciation in human serum. first investigations showed that iodine species nearly quantitatively were eluted in the void volume. the result indicated that protein-linked thyroid hormones were not interacting with the stationary phase, thus being not retained. investigations were performed about t4-tbg (thyroxin-thyroxin-binding globulin) complex generation and its retention during c ... | 2000 | 11314990 |
| endocrine problems in the chronically critically ill patient. | the endocrine adaptations to critical illness are varied. in the diabetic patient, counterregulatory hormones predispose to insulin resistance and hyperglycemia, a derangement accentuated by the use of glucocorticoids and enteral or parenteral nutrition. thyroid abnormalities include the euthyroid sick syndrome, which may manifest as a low t3, low t4, low tsh, or all three. illness in patients with pre-existing hypothyroidism or hyperthyroidism may precipitate myxedema coma or thyroid storm, res ... | 2001 | 11315456 |
| voluntary head stabilisation in space during oscillatory trunk movements in the frontal plane performed before, during and after a prolonged period of weightlessness. | the ability to voluntarily stabilise the head in space exhibited by two subjects during lateral rhythmic oscillations of the trunk has been investigated before, during and after a prolonged period of microgravity (microg) exposure. in flight acquisitions were performed onboard the core module of the russian space station mir as part of the t4 "human posture in microgravity" experiment of the 179-days esa-rka mission euromir-95. data collection and kinematic analysis were performed by means of a ... | 2001 | 11315545 |
| the relation of ct-determined tumor parameters and local and regional outcome of tonsillar cancer after definitive radiation treatment. | to investigate the value of ct-derived tumor parameters as predictor of local and regional outcome of tonsillar squamous cell carcinoma treated by definitive radiation therapy. | 2001 | 11316544 |
| structural and thermodynamic analysis of the binding of solvent at internal sites in t4 lysozyme. | to investigate the structural and thermodynamic basis of the binding of solvent at internal sites within proteins a number of mutations were constructed in t4 lysozyme. some of these were designed to introduce new solvent-binding sites. others were intended to displace solvent from preexisting sites. in one case val-149 was replaced with alanine, serine, cysteine, threonine, isoleucine, and glycine. crystallographic analysis shows that, with the exception of isoleucine, each of these substitutio ... | 2001 | 11316887 |
| subacute thyroiditis in a single lobe. | a 33-year-old woman with no history of thyroid disease reported pain in her neck and a sore throat. on physical examination, the thyroid gland was palpable. serum t3 and t4 levels were increased, and the thyroid-stimulating hormone level was decreased. thyroid scintigraphy with tc-99m pertechnetate revealed nonvisualization of the left lobe of the thyroid. ultrasonographic examination confirmed the presence of the left thyroid lobe. fine-needle aspiration biopsy revealed thyroiditis of the left ... | 2001 | 11317018 |
| hormonal and metabolic response in elite female gymnasts undergoing strenuous training and supplementation with supro brand isolated soy protein. | this study evaluates the metabolic and hormonal response in elite female gymnasts undergoing strenuous training and supplementation with supro brand isolated soy protein. methods. | 2001 | 11317154 |
| a placebo-controlled, double-blind, randomised trial of magnesium-pyridoxal-5'-phosphate-glutamate for hypercholesterolaemia and other clinical-chemical risk factors of cardiovascular disease in a primary care setting. | lipid-lowering drugs are extensively used in primary care to reduce the risk of cardiovascular disease (cvd). apart from high total cholesterol (tc), several other clinical-chemical variables are associated with the risk of cvd. magnesium-pyridoxal-5'-phosphate-glutamate (mppg) has been found to have a positive influence on tc levels and other clinical-chemical values in some selected populations. | 2001 | 11317472 |
| presence and possible significance of immunohistochemically demonstrable metallothionein expression in pterygium versus pinguecula and normal conjunctiva. | to investigate metallothionein (mt) expression in pterygium, pinguecula and normal conjunctiva and define its possible significance in this area of the eye. in order to further elucidate the mechanism of mt expression we correlated it with lymphocyte subpopulations (t4, t8), macrophages (cd68), langerhans' cells (s100) and the proliferation-associated indices (pcna, ki67). | 2001 | 11318304 |
| [t3 and t4 cancer of the oral cavity, surgical treatment with oral tongue resection]. | amputation of the oral tongue is required to treat t3 and t4 bilateral tumors of the anterior two third of the tongue with or without extension to the floor of the mouth. this partial glossectomy was performed initially for 27 patients and as salvage therapy for 35 patients with recurrent diseases. the reconstruction required a flap in all cases, including 8 microvascular free flaps. two months after surgery, two third of patients had a satisfactory swallowing hability. the functional results we ... | 2001 | 11319407 |
| [use of neck platysma myocutaneous flap for reconstruction substance defects after surgical resection of oropharyngeal tumors. report of 70 cases]. | we analyzed our experience with the cervical platysma myocutaneous flap and reviewed the pertinent literature. we evaluated the harvesting technique, surgical indications, reliability of the flap, its advantages and drawbacks compared with other reconstruction techniques and assessed survival and local control. | 2001 | 11319411 |
| prognosis of t4 gastric carcinoma patients: an appraisal of aggressive surgical treatment. | despite precipitous drop in the incidence of gastric carcinoma in japan, it is still one of the leading causes of death associated with malignant disease. once the contiguous organs are involved the prognosis becomes dismal. prognostic factors governing the survival of patients with t4 gastric carcinoma remain unclear. | 2001 | 11320520 |
| [long-term observations on type distribution of clinical isolated of beta-hemolytic streptococci in sapporo. i. t-type distribution of group a hemolytic streptococci during the thirty one-year period between 1969 and 1999]. | during the thirty one-year period between 1969 and 1999, a total of 6,876 strains of group a streptococci isolated in sapporo city general hospital were classified by t-agglutination method. this research consisted of 5,866 strains of scarlet fever-patients (scarlet fever-strains), 450 of child-patients (child-strains), 141 of adult-patients (adult-strains) and 409 strains of the indistinct. the results were summarized as follows: 1. total of 6,866 strains were classified to sixteen kinds of t-t ... | 2001 | 11321775 |
| stereotactic navigation for placement of pedicle screws in the thoracic spine. | pedicle screw fixation in the lumbar spine has become the standard of care for various causes of spinal instability. however, because of the smaller size and more complex morphology of the thoracic pedicle, screw placement in the thoracic spine can be extremely challenging. in several published series, cortical violations have been reported in up to 50% of screws placed with standard fluoroscopic techniques. the goal of this study is to evaluate the accuracy of thoracic pedicle screw placement b ... | 2001 | 11322437 |
| self-assembled pearling structure of long duplex dna with histone h1. | we report that complexes of giant dna molecules with histone h1 proteins form a pearl necklace-like structure when the complexes are prepared by natural dilution from a high-salt solution (2 m nacl) to a low-salt solution (0.2 m and 50 mm nacl). we performed real-time observations on the conformational changes of individual t4 phage dna (166 kb) molecules in bulk solution by fluorescence microscopy. to identify h1-binding regions on individual dna molecules, we also performed immunofluorescence ... | 2001 | 11322878 |
| nitric oxide is involved in arsenite inhibition of pyrimidine dimer excision. | arsenite is a human carcinogen reported to inhibit dna repair. the binding of arsenite to functional thiol groups of dna repair enzymes has in the past been suggested as a possible mechanism for the effect of arsenite on dna repair. however, recent studies indicate that reactive oxygen species and nitric oxide are involved in arsenite toxicity. this research aims to elucidate the role of these possible mechanisms in the inhibition of uv-induced dna repair by arsenite. as arsenite inhibits uv-dna ... | 2001 | 11323388 |
| a comparison between automatically generated linear and parabolic tetrahedra when used to mesh a human femur. | finite element models of bone segments generated from computed tomography data using automatic mesh generation algorithms are becoming common not only in research but also in clinical applications such as computer aided orthopaedic surgery. especially in the case of the latter application, the models cannot be verified against an experimental measurement, therefore their inherent accuracy should be well known before drawing conclusions based on the calculated results. this study was carried out ... | 2001 | 11323989 |
| thermodynamic molecular switch in macromolecular interactions. | it is known that most living systems can live and operate optimally only at a sharply defined temperature, or over a limited temperature range, at best, which implies that many basic biochemical interactions exhibit a well-defined gibbs free energy minimum as a function of temperature. the gibbs free energy change, deltag(o) (t), for biological systems shows a complicated behavior, in which deltag(o)(t) changes from positive to negative, then reaches a negative value of maximum magnitude (favora ... | 2000 | 11325035 |
| enzymatic mutation detection method evaluated for detection of p53 mutations in cdna from breast cancers. | rapid, reproducible, and easily run methods with high sensitivity and specificity are required for mutation screening of clinical samples. we evaluated the enzymatic mutation detection (emd(tm)) method by analysis of archival cdna from 203 breast cancer patients and comparison with results of cdna-based sequencing of the tumor suppressor gene p53. | 2001 | 11325884 |
| use of subgenic 18s ribosomal dna pcr and sequencing for genus and genotype identification of acanthamoebae from humans with keratitis and from sewage sludge. | this study identified subgenic pcr amplimers from 18s rdna that were (i) highly specific for the genus acanthamoeba, (ii) obtainable from all known genotypes, and (iii) useful for identification of individual genotypes. a 423- to 551-bp acanthamoeba-specific amplimer asa.s1 obtained with primers jdp1 and jdp2 was the most reliable for purposes i and ii. a variable region within this amplimer also identified genotype clusters, but purpose iii was best achieved with sequencing of the genotype-spec ... | 2001 | 11326011 |
| immunohistochemical examinations (ki67, p53, nm23) and dna cytophotometry in bladder cancer. | bladder cancer is clinically characterized by a high recurrence rate for superficial tumours up to 70% and by the invasiveness of advanced bladder cancer. to learn more about the biological behaviour of an individual bladder cancer different tumour markers have been investigated. the aim of our study was to compare the potential of aggression of both superficial and invasive bladder tumours by means of the proliferation marker ki67, the tumour suppressor gene p53, the non metastasizing protein n ... | 2000 | 11326661 |
| [effects of tripchlorolide (t4) of tripterygium wilfordii hook on the production of immunoglobulins by peripheral blood mononuclear cells and by synovial cells of rheumatoid arthritis patients in vitro]. | tripchlorolide(t4) is an active ingredient recently isolated from tripterygium wilfordii hook. the in vitro effects of t4 on the peripheral blood mononuclear cells(pbmc) of healthy persons(n = 10), the digested single synovium cells(dssc) (n = 3) and pbmc(n = 6) from ra patients on production of immunoglobulins (ig) were studied using elisa method. the results showed that t4 at concentrations from 5 ng.ml-1 to 35 ng.ml-1 significantly reduced the production of ig by pwm-stimulated pbmc of health ... | 1997 | 11327015 |
| comparison of some different methods for analysis of thyroid autoantibodies: importance of thyroglobulin autoantibodies. | blood samples from 141 children and adolescents were used to evaluate differences between commercial kits and radioimmunoassay (ria) methods for detecting thyroid autoantibodies. thyroglobulin autoantibodies (tg-ab) were analyzed with a hemagglutination kit and a ria; thyroid peroxidase autoantibodies (tpo-ab) were measured with a gelagglutination assay and a ria. the results of the antibody tests were compared with thyroid function tests (triiodothyronine [t3], thyroxine [t4], thyrotropin [tsh] ... | 2001 | 11327618 |
| a pituitary tumor in a patient with thyroid hormone resistance: a diagnostic dilemma. | resistance to thyroid hormone (rth) is due to mutations in the beta-isoform of the thyroid hormone receptor (tr-beta). rth patients display inappropriate secretion of thyrotropin-releasing hormone (trh) from the hypothalamus and thyrotropin (tsh) from the anterior pituitary, despite elevated levels of thyroid hormone thyroxine (t4) and triiodothyronine (t3). thyrotropin-secreting tumors are presumed to represent clonal expansion of abnormal cells. because the diagnosis of tsh-secreting tumors te ... | 2001 | 11327621 |
| conformational flexibility in t4 endonuclease vii revealed by crystallography: implications for substrate binding and cleavage. | the structure of the n62d mutant of the junction-resolving endonuclease vii (endovii) from phage t4 has been refined at 1.3 a, and a second wild-type crystal form solved and refined at 2.8 a resolution. comparison of the mutant with the wild-type protein structure in two different crystal environments reveals considerable conformational flexibility at the dimer level affecting the substrate-binding cleft, the dimerization interface and the orientation of the c-terminal domains. the opening of th ... | 2001 | 11327769 |
| variations in urinary iodine excretion and thyroid function. a 1-year study in healthy men. | the iodine intake level in a population is determined in cross-sectional studies. a fraction of samples with iodine content below a certain level, e.g. 25 microg/l, may suggest iodine deficiency in part of the population. however, urinary iodine varies considerably from day to day and the fraction of low samples caused by dispersion remains unsettled. | 2001 | 11331211 |
| effects of l-thyroxine administration, tsh-receptor antibodies and smoking on the risk of recurrence in graves' hyperthyroidism treated with antithyroid drugs: a double-blind prospective randomized study. | in graves' hyperthyroidism treated with antithyroid drugs (atd), the overall relapse rate reaches 30-50% following atd discontinuation. conflicting results have previously been reported with regard to the usefulness of combining atd with thyroxine (l-t4), and thereafter maintaining l-t4 treatment after atd withdrawal. also, clinicians are in search of useful parameters to predict the risk of a recurrence of hyperthyroidism after atd treatment. | 2001 | 11331213 |
| dna ligase i and proliferating cell nuclear antigen form a functional complex. | dna ligase i is responsible for joining okazaki fragments during dna replication. an additional proposed role for dna ligase i is sealing nicks generated during excision repair. previous studies have shown that there is a physical interaction between dna ligase i and proliferating cell nuclear antigen (pcna), another important component of dna replication and repair. the results shown here indicate that human pcna enhances the reaction rate of human dna ligase i up to 5-fold. the stimulation is ... | 2001 | 11331287 |
| addition of oxaliplatin to continuous fluorouracil, l-folinic acid, and concomitant radiotherapy in rectal cancer: the lyon r 97-03 phase i trial. | oxaliplatin could increase the efficacy of fluorouracil (5-fu)/folinic acid chemoradiotherapy in rectal cancer. we tested three dose levels to identify a feasible oxaliplatin dose for combination therapy. | 2001 | 11331322 |
| neurokinin-1 receptor-immunoreactive neurons of the ventral respiratory group in the rat. | the rostral end of the ventral respiratory group (vrg) contains neurons that are intensely neurokinin-1 receptor (nk1r) immunoreactive (ir). it has been theorized that some of these cells might be critical to respiratory rhythmogenesis (gray et al. [1999] science 286:1566-1568). in the present study we determined what major transmitter these nk1r-ir cells make and whether they are bulbospinal or propriomedullary. nk1r-ir neurons were found in the vrg between bregma levels -11.7 and -13.6 mm. the ... | 2001 | 11331521 |
| structure of hjc, a holliday junction resolvase, from sulfolobus solfataricus. | the 2.15-a structure of hjc, a holliday junction-resolving enzyme from the archaeon sulfolobus solfataricus, reveals extensive structural homology with a superfamily of nucleases that includes type ii restriction enzymes. hjc is a dimer with a large dna-binding surface consisting of numerous basic residues surrounding the metal-binding residues of the active sites. residues critical for catalysis, identified on the basis of sequence comparisons and site-directed mutagenesis studies, are clustere ... | 2001 | 11331763 |
| [chairman of the german society for child psychiatry and educational therapeutics in world war ii]. | hans heinze was one of the three experts on child euthanasia in the "reichsausschuss zur wissenschaftlichen erfassung erb- und anlagebedingter schwerer leiden" and head of the first "kinderfachabteilung" in brandenburg-görden, germany. he was one of the experts in the euthanasia "aktion t4". he became nominated president of the "deutsche gesellschaft für kinderpsychiatrie und heilpädagogik" after paul schröder who died in 1941. the documents about his function exist in five german archives. | 2001 | 11332133 |
| high-dose radiation therapy and neoadjuvant plus concomitant chemotherapy with 5-fluorouracil and cisplatin in patients with locally advanced squamous-cell anal canal cancer: final results of a phase ii study. | to analyse toxicity and response to a new scheme of neoadjuvant chemotherapy (ct) and concomitant radiochemotherapy (rt-ct) for locally advanced anal canal squamous-cell carcinoma (acc). | 2001 | 11332154 |
| [pregnancy (conception) in hyper- or hypothyroidism]. | pregnancy is accompanied by changes in thyroid function. due to the increased synthesis of thyroid binding globulin and the thyroid-stimulating effect of human chorionic gonadotrophin (hcg), serum concentrations of thyroid hormones will increase in the first trimester of pregnancy (total t4, t3). free t4 levels decrease during the latter half of pregnancy. hyperthyroidism during pregnancy is usually due to graves' disease. definitive therapy may be considered for cases prior to pregnancy, althou ... | 2001 | 11332254 |
| recognition and specific degradation of bacteriophage t4 mrnas. | gene 61.5 of bacteriophage t4 has a unique role in gene expression. when this gene is mutated, mrnas of many late genes are rapidly degraded, resulting in late-gene silencing. here, we characterize an extragenic suppressor, ssf5, of a gene 61.5 mutation. ssf5 was found to be an amber mutation in mota, which encodes a transcription activator for t4 middle genes. when this gene is mutated, both degradation and specific cleavage of late-gene mrna is induced after a delay, as exemplified by soc mrna ... | 2001 | 11333214 |
| repair of topoisomerase-mediated dna damage in bacteriophage t4. | type ii topoisomerase inhibitors are used to treat both tumors and bacterial infections. these inhibitors stabilize covalent dna-topoisomerase cleavage complexes that ultimately cause lethal dna damage. a functional recombinational repair apparatus decreases sensitivity to these drugs, suggesting that topoisomerase-mediated dna damage is amenable to such repair. using a bacteriophage t4 model system, we have developed a novel in vivo plasmid-based assay that allows physical analysis of the repai ... | 2001 | 11333215 |
| identification of a mutant dna polymerase delta in saccharomyces cerevisiae with an antimutator phenotype for frameshift mutations. | we propose that a beta-turn-beta structure, which plays a critical role in exonucleolytic proofreading in the bacteriophage t4 dna polymerase, is also present in the saccharomyces cerevisiae dna pol delta. site-directed mutagenesis was used to test this proposal by introducing a mutation into the yeast pol3 gene in the region that encodes the putative beta-turn-beta structure. the mutant dna pol delta has a serine substitution in place of glycine at position 447. dna replication fidelity of the ... | 2001 | 11333228 |
| clinical evaluation of nine hollow-fibre membrane oxygenators. | in a comparative study we investigated the performance characteristics of nine hollow-fibre oxygenators. in a clinical setting, 10 units of each type of oxygenator were tested for oxygen exchange, transoxygenator pressure drop, heat exchanger performance and blood trauma. the oxygenators included are maxima prf plus, affinity, forte, affinity nt, quantum, optima, capiox 1.8, hilite and quadrox. ninety patients scheduled for elective coronary bypass surgery were enrolled in the study. cardiotomy ... | 2001 | 11334201 |
| domain structure and mutational analysis of t4 polynucleotide kinase. | t4 polynucleotide kinase (pnk) is the founding member of a family of 5'-kinase/3'-phosphatase enzymes that heal broken termini in rna or dna by converting 3'-po(4)/5'-oh ends into 3'-oh/5'-po(4) ends, which are then suitable for sealing by rna or dna ligases. here we employed site-directed mutagenesis and biochemical methods to dissect the domain structure of the homotetrameric t4 pnk protein and to localize essential constituents of the apparently separate active sites for the 5'-kinase and 3'- ... | 2001 | 11335730 |
| intra-arterial plus i.v. chemotherapy for advanced bulky squamous cell carcinoma of the buccal mucosa. | from july 1994 to december 1996, 41 patients with previously untreated, advanced bulky squamous cell carcinoma arising from the buccal mucosa (bscc) were enrolled. all patients were males with a median age of 47 years (range 29-72). the tumor extent was stage iii/iv: three of 38, t4: 85%, n2-3: 20%. patients were initially scheduled to receive intra-arterial (i.a.) chemotherapy, followed by i.v. chemotherapy and regional therapy. the i.a. chemotherapy catheter was properly placed by external car ... | 2001 | 11335789 |
| hashimoto's disease during interferon-alpha therapy in a patient with pre-treatment negative anti-thyroid autoantibodies and with the specific genetic susceptibility to the thyroid disease. | the authors described a case of hashimoto's disease during interferon-alpha (ifn-alpha) treatment for chronic viral c hepatitis in a patient with the specific genetic susceptibility associated with the thyroid disease. | 2001 | 11335880 |
| enteral nutrition during the treatment of head and neck carcinoma: is a percutaneous endoscopic gastrostomy tube preferable to a nasogastric tube? | multimodality treatments for patients with squamous cell head and neck carcinoma often produce significant mucositis and dysphagia, mandating enteral nutritional support. patient preference has resulted in the increasing use of percutaneous endoscopic gastrostomy (peg) tubes rather than nasogastric (ng) tubes. anecdotal observations of prolonged peg dependence and of a need for pharyngoesophageal dilatation in peg patients prompted a retrospective review of the use of both types of feeding tubes ... | 2001 | 11335904 |
| in vitro replication and mutagenesis of a novel reversion vector with selective dna damage in the supf gene. | the ability to detect the most common type of uv-induced mutation, the c to t transition, at the previously characterized hotspot at position 99 of the supf gene has been demonstrated in a selectively irradiated reversion vector, pls189(rev). the supf region was amplified, irradiated with 500j/m(2) uvc or unirradiated, and ligated into the pls189(rev) plasmid. a portion of ligated product plasmid containing the irradiated fragment was sensitive to nicking by t4 endonuclease v, indicating the pre ... | 2001 | 11336980 |
| inhalation of [123i]alpha1-protease inhibitor: toward a new therapeutic concept of alpha1-protease inhibitor deficiency? | the alpha1-protease inhibitor (alpha1-pi) is separated from human serum and is therefore extremely expensive. because only 2%-3% concentrates in the lung after intravenous administration, inhalational therapy for alpha1-pi deficiency would seem likely to be better. the aims of this study were therefore to determine the pattern of deposition of inhaled alpha1-pi labeled with 123i and measure the amount deposited in the lungs. | 2001 | 11337570 |
| idiopathic spinal cord herniation associated with intervertebral disc extrusion: a case report and review of the literature. | a case of idiopathic spinal cord herniation is reported, and the literature is reviewed. | 2001 | 11337631 |
| negative correlation between plasma thyroid hormone levels and chlorinated hydrocarbon levels accumulated in seals from the coast of hokkaido, japan. | chlorinated hydrocarbon (chc) levels in the blubber of larga seals (phoca largha) and ribbon seals (phoca fasciata) collected from the coastal waters of hokkaido, japan, were determined in order to assess the hormonal effects of chc exposure in free-ranging pinnipeds. plasma thyroid hormone levels, including total thyroxine (t4), free thyroxine (free t4), total triiodothyronine (t3), and free triiodothyronine (free t3), were also measured. higher concentrations of polychlorinated biphenyl congen ... | 2001 | 11337873 |
| cortical representation of the vestibular system as evidenced by brain electrical activity mapping of vestibular late evoked potentials. | we examined the space and temporal distributions of the rotatory evoked brain electrical activity patterns (brain electrical activity mapping of vestibular evoked potentials [vestep]) in humans. we performed a longitudinal scalp line analysis, transversal line analysis, and clockwise/counterclockwise rotation analysis of the vestep principal components in 75 healthy persons aged 22 to 30 years (mean: 25.8). we found that the shortest vestep latencies and the highest amplitudes were registered in ... | 2001 | 11338651 |
| [several hormonal indices during hemofiltration in patients with acute kidney failure]. | to study hormonal aspects of hemofiltration (hf) in acute renal failure (arf). | 2001 | 11338856 |
| plasma levels of persistent organohalogens and hormone levels in adult male humans. | in this study the authors assessed the possible relationship between high dietary exposure to persistent organohalogens (ohs) through fatty fish from the baltic sea and hormone levels in adult men. blood samples were drawn from 110 men who consumed varying amounts of fish (i.e., 0-32 meals per month) for analysis of plasma levels of 18 polychlorinated biphenyl (pcb) congeners, 5 hydroxy-pcbs, 1,1,1-trichloro-2,2-bis(4-chlorophenyl)-ethane (p,p'-ddt), 1,1-dichloro-2,2-bis(4-chlorophenyl)-ethene ( ... | 2001 | 11339677 |
| fatigue and hyponatremia in a 75-year-old woman: unusual presentation of hypophysitis. | a 75-year-old woman presented with general fatigue progressing to somnolence. laboratory tests showed marked hyponatremia. tsh in the normal range, but low levels of free t3 and free t4. evaluation of pituitary hormones and magnetic resonance imaging of the pituitary unmasked findings characteristic for hypophysitis with secondary adrenal insufficiency and secondary hypothyroidism. hormonal substitution with hydrocortisone and levothyroxine resulted in rapid improvement of all symptoms and signs ... | 2001 | 11341300 |
| prognostic factors in node-negative squamous cell carcinoma of the thoracic esophagus. | among several clinicopathological factors influencing the outcome of patients with esophageal carcinoma, the presence or absence of lymph node metastasis is the most important. prognostic indicators, however, in patients without lymph node involvement have not been fully understood. | 2000 | 11341595 |
| crystallographic analysis of a novel complex of actinomycin d bound to the dna decamer cgatcgatcg. | the potent anticancer drug actinomycin d (actd) acts by binding to dna, thereby interfering with replication and transcription. actd inhibits rna polymerase far more specifically than dna polymerase. such discrimination is not easily understood by the conventional dna binding mode of actd. we have solved and refined at 1.7 a resolution the crystal structure of actd complexed to cgatcgatcg, which contains no canonical gpc binding sequence. the crystal data are space group p4(3)2(1)2, a = b = 47.0 ... | 2001 | 11341823 |
| generation of macrophages from early t progenitors in vitro. | early t progenitors in the thymus have been reported to have the capacity to develop into b cells, thymic dendritic cells, and nk cells. here we describe conditions that induce early t progenitors to develop into macrophages. initially, we observed that early t progenitors could be induced to develop into macrophages by cytokines produced from a thymic stromal cell line, tfgd, and later we found that the cytokine mixture of m-csf plus il-6 plus il-7 also induced macrophage differentiation from p ... | 2001 | 11342611 |
| role of esophagectomy in treatment of esophageal carcinoma with clinical evidence of adjacent organ invasion. | with carcinoma of the thoracic esophagus, clinical evidence of invasion of adjacent organs (t4) indicates a highly advanced stage, and most surgeons avoid esophagectomy. although the therapeutic strategy for such disease is generally selected based on preoperative evaluation and intraoperative inspection, their accuracy and the relation to survival outcomes after esophagectomy have seldom been analyzed on the basis of exact histopathologic evidence. we performed esophagectomy, with perioperative ... | 2001 | 11343176 |
| thyroxine and the treatment of affective disorders: an overview of the results of basic and clinical research. | eight open clinical trials conducted by 7 different study groups and including 78 patients have all demonstrated that augmentation with supraphysiological doses of thyroxine (t4) has antidepressant and prophylactic effects in roughly 50% of patients completely resistant to all other antidepressant and prophylactic therapies. beneficial effects have been observed in unipolar and bipolar (rapid-cycling and non-rapid-cycling) patients, but only when an antidepressant or prophylactic drug was admini ... | 2000 | 11343592 |
| mental improvement after replacement therapy with thyroxine plus triiodothyronine: relationship to cause of hypothyroidism. | we treated 26 hypothyroid women - 11 with autoimmune thyroiditis and 15 who had been treated for thyroid cancer - with their usual dose of thyroxine (t4) or with a regimen in which 50 &mgr;g of t4 had been replaced by 12.5 &mgr;g of triiodothyronine (t3). patients were first randomly assigned to one regimen for 5 wk and then to a second regimen for an additional 5 wk. the substitution of t3 for a portion of t4 caused expected changes in concentrations of thyroid hormones and thyroid-stimulating ... | 2000 | 11343593 |
| a multiple ligand-binding radioimmunoassay of diiodotyrosine. | a radioimmunoassay has been developed for the measurement of 3,5-diiodo-l-tyrosine (dit) in serum. dit was coupled to porcine thyroglobulin (ptg) with a molar ratio of 205:1. rabbits were immunized with 1 mg of immunogen emulsified in complete freund's adjuvant. sera were screened for their ability to bind trace amounts of [125i]dit. a serum that bound 40% of the tracer at a final dilution of 1:1,750 was used in the assay. assay specificity was improved by the use of thyroxine (t4)-binding globu ... | 1974 | 11344555 |
| a new mri technique for imaging scoliosis in the sagittal plane. | the purpose of the present study was to introduce a new magnetic resonance imaging (mri) procedure showing the whole spine in a coronal and sagittal plane, and to study the assessment of sagittal cobb angle measurements using this technique. prospectively we studied 32 patients (average age 14.8 years) with idiopathic scoliosis (mean thoracic cobb angle 33 degrees on radiograph) and 18 patients (average age 14.5 years) without scoliosis. the mri investigation was carried out in a standard supine ... | 2001 | 11345631 |
| alterations of cognitive functions induced by exogenous application of thyroid hormones in healthy men: a double-blind cross-over study using event-related brain potentials. | the influence of experimentally induced subclinical hyperthyroidism on cognitive functions was tested using exogenous thyroxine or placebo. in a double-blind cross-over design, 24 healthy young men received either 300 microg of levothyroxine or placebo for two consecutive 3-week periods. determination of thyrotropin (tsh), total triiodothyronine (t3), thyroxine (t4), and sex hormone binding globulin (shbg) confirmed the induction of subclinical hyperthyroidism. three weeks after treatment with p ... | 2001 | 11349839 |
| increased stability and lifetime of the complex formed between dna and meta-phenyl-substituted hoechst dyes as studied by fluorescence titrations and stopped-flow kinetics. | the large increase in fluorescence upon binding of five para- and meta-phenyl substituted hydroxy and methoxy derivatives of the hoechst dye with poly[d(a-t)], d(cgcgaattcgcg)2, and its corresponding t4-looped 28-mer hairpin was used to monitor the binding by equilibrium titrations and by stopped-flow kinetics. the affinity increases in the same order for the three dnas: p-oh<m-och3, p-oh<m-oh<m-oh, p-och3<bis-m-oh. the association constants k(a) are three to 11 times larger for the aatt site th ... | 2001 | 11350167 |
| what is the average conformation of bacteriophage t4 lysozyme in solution? a domain orientation study using dipolar couplings measured by solution nmr. | lysozyme from t4 bacteriophage is comprised of two domains that are both involved in binding substrate. although wild-type lysozyme has been exclusively crystallized in a closed form that is similar to the peptidoglycan-bound conformation, a more open structure is thought to be required for ligand binding. to determine the relative arrangement of domains within t4 lysozyme in the solution state, dipolar couplings were measured in several different dilute liquid crystalline media by solution nmr ... | 2001 | 11350172 |
| lack of peripheral analgesia mediated by intraplantar administration of neostigmine in carrageenan-injected rats. | the aim of the study was to test the peripheral analgesic action of neostigmine. intraplantar administration of neostigmine was studied in carrageenan-injected rats. | 2001 | 11350472 |
| polyvinyl pyrrolidone-iodine liposome hydrogel improves epithelialization by combining moisture and antisepis. a new concept in wound therapy. | moist treatment of wounds has been shown to improve epithelialization, however at an increased risk of bacterial infection. in this monocentric, randomized, open, phase ii pilot study of polyvinyl pyrrolidone-iodine, a well-established topical antiseptic was tested in a new liposomal complexed form in patients receiving meshed skin grafts after burns or reconstructive procedures. mesh skin graft sites of 36 patients were dressed either with the new polyvinyl-pyrrolidone-iodine liposome hydrogel ... | 2001 | 11350649 |
| preparation and properties of a selenium-containing catalytic antibody as type i deiodinase mimic. | conversion of thyroxine (t4) to 3,5,3'-triiodothyronine is an essential first step in controlling thyroid hormone action. type i deiodinase (di) can catalyze the conversion to produce the bulk of serum 3,5,3'-triiodothyronine. acting as a mimic of di, a selenium-containing catalytic antibody (se-4c5) prepared by converting the serine residues of monoclonal antibody 4c5 raised against t4 into selenocysteines, can catalyze the deiodination of t4 with dithiothreitol (dtt) as cosubstrate. the mimic ... | 2001 | 11350963 |
| immunohistochemical detection of the anti-apoptosis protein, survivin, predicts survival after curative resection of stage ii colorectal carcinomas. | this study examined the role of survivin protein, a novel inhibitor of apoptosis, in determining prognosis after curative resection of stage ii colorectal carcinomas. | 2001 | 11352303 |
| stabilization of short collagen-like triple helices by protein engineering. | recombinant expression of collagens and fragments of collagens is often difficult, as their biosynthesis requires specific post-translational enzymes, in particular prolyl 4-hydroxylase. although the use of hydroxyproline-deficient variants offers one possibility to overcome this difficulty, these proteins usually differ markedly in stability when compared with the hydroxyproline-containing analogs. here, we report a method to stabilize collagen-like peptides by fusing them to the n terminus of ... | 2001 | 11352592 |
| rearrangement of structured rna via branch migration structures catalysed by the highly related dead-box proteins p68 and p72. | rna helicases, like their dna-specific counterparts, can function as processive enzymes, unwinding rna with a defined step size in a unidirectional fashion. recombinant nuclear dead-box protein p68 and its close relative p72 are reported here to function in a similar fashion, though the processivity of both rna helicases appears to be limited to only a few consecutive catalytic steps. the two proteins resemble each other also with regard to other biochemical properties. we have found that both p ... | 2001 | 11353078 |