Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| congruent spinopelvic alignment on standing lateral radiographs of adult volunteers. | radiographs of 75 healthy volunteers were measured to decide parameters and ranges for "congruent" sagittal spinopelvic alignments using the pelvic radius technique. a subset of 30 of the volunteers subsequently had a second radiograph to assess for changes in the repeated measurements. | 2000 | 11064527 |
| the perception of lexical tone in mambila. | the issue of the perception of lexical tone has been addressed mainly through studies of southeast asian languages which feature phonological contour tones as well as level tones. little attention has been paid to african languages which have, almost exclusively, only level tones. this paper examines tone perception in mambila, a benue-congo language with four level lexical tones. a categorization experiment was run to determine some of the salient aspects of the perceptual nature of these tones ... | 2000 | 11064955 |
| salvage radiotherapy for prostate cancer recurrence after cryosurgical ablation. | to retrospectively determine the outcome of patients treated with salvage three-dimensional conformal radiotherapy (rt) for prostate cancer recurrence after cryosurgical ablation of the prostate (csap). biochemical control rates and morbidity were analyzed. | 2000 | 11068312 |
| [clinical epidemiology of basedow's disease in belgium]. | graves' disease (gd) patients treated with antithyroid drugs (atd) have overall relapse rates of 30-50% after atd discontinuation. conflicting data have been reported with regard to the usefulness of adding thyroxine (i-t4) during and after atd treatment. also, clinicians are still in search of useful factors to predict remission/recurrence after atd withdrawal. eighty two consecutive patients were treated with atd for 15 months, combined with 12 months of i-t4. then, patients were randomized (p ... | 2000 | 11068483 |
| thyroxin and thiouracyl influence upon some biochemical parameters in chicken thymuses. | administration of l-thyroxin in a dose of 30 micrograms/chicken, i.m. and 2-thiouracyl in a dose of 0.3 mg/chicken, i.m. for an interval of 3 days, determined, at the thymus level, increases of total protein content in the tu group, in parallel with decreases of the nitrogen of free amino acids level in the tx group, increases of dna content in the tx group and an increase of the thymus weight in both groups. modifications are due to the action of thyroxin, on the one hand, and on the other to t ... | 1999 | 11068610 |
| randomized, double-blind clinical trial, controlled with placebo, of the toxicology of chronic melatonin treatment. | the objective of the present study was to assess the toxicology of melatonin (10 mg), administered for 28 days to 40 volunteers randomly assigned to groups receiving either melatonin (n = 30) or placebo (n = 10) in a double-blind fashion. the following measurements were performed: polysomnography (psg), laboratory examinations, including complete blood count, urinalysis, sodium, potassium and calcium levels, total protein levels, albumin, blood glucose, triglycerides, total cholesterol, high-den ... | 2000 | 11068941 |
| [the analysis of the selected epidemiological aspects in patients with inhalant allergy treated at the allergology outpatient clinic in zabrze]. | the study was designed to analyse the selected epidemiological aspects in patients with inhalant allergy treated at the allergology outpatient clinic in zabrze from 1989 till 1994. the analysis was based on the data from the files of 11,540 patients. the data of the pollinotic patients (1571 subjects) and of those with bronchial asthma (521 subjects) were analysed with regard to frequency of the diseases, place of residence (urban or country area), sex, date of birth, age when the illness had oc ... | 2000 | 11070761 |
| [operative stress during aorto-bifemoral reconstruction as dependent on a type of anesthesia]. | abdominal aortic reconstruction surgery in patients with arteriosclerosis obliterans usually has been carried out under standard intratracheal anaesthesia and only a some few centres have included continuous suprameningeal anaesthesia. on the basis of that scanty data it seems that combined intratracheal and suprameningeal anaesthesia might have some advantages over the standard anaesthesia owing to reducing general symptoms of perioperative stress. the aim of this study was to investigate the o ... | 2000 | 11070762 |
| prevention of renal cell carcinoma by active vitamin d3. | we studied the serum levels of 1,25-dihydroxyvitamin d [1,25(oh)2d (vit d)] in patients with renal cell carcinoma (rcc) and the influence of 1,25(oh)2d3 (vit d3) on gap junctional intercellular communication (gjic) during carcinogenesis. the serum vit d levels were measured by a competitive protein-binding assay using the chromatographic method. using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (mtt) assay, noncytotoxic concentrations of vit d3 and the tumor promoters n-ni ... | 2000 | 11071463 |
| squamous cell carcinoma of the oropharynx: ki-67 and p53 can identify patients at high risk for local recurrence after surgery and postoperative radiotherapy. | to assess the prognostic value of biologic (p53, ki-67) and clinical factors in squamous cell carcinoma of the oropharynx after radical surgery and postoperative radiotherapy (rt). | 2000 | 11072161 |
| prognostic significance of postchemoradiation stage following preoperative chemotherapy and radiation for advanced/recurrent rectal cancers. | to evaluate the prognostic significance of postchemoradiation pathologic stage and implications for further therapy following preoperative chemoradiation and surgery for advanced/recurrent rectal cancer. | 2000 | 11072165 |
| bcmsun, a candidate gene for b-cell chronic lymphocytic leukemia and mantle-cell lymphoma, has an independently expressed homolog on 1p22-p31, bcmsun-like. | the most frequent chromosomal imbalance in b-cell chronic lymphocytic leukemia (b-cll) and mantle-cell lymphoma (mcl) is loss of material from 13q14.3. bcmsun (previously leu2, t4, cdna 1b4) is one of 3 proposed candidate genes isolated from the minimally deleted region. we identified a homolog of bcmsun, termed bcmsunl (for bcmsun-like). radiation-hybrid mapping with a pcr-amplified fragment and fluorescence in situ hybridization with 2 pac clones containing coding information for bcmsunl revea ... | 2000 | 11072235 |
| high complete pathological response in locally advanced breast cancer using paclitaxel and cisplatin. | in an earlier study, we have demonstrated a high response rate in metastatic breast cancer using paclitaxel (p) and cisplatin (c). a phase ii study using the same regimen (pc) has been conducted in locally advanced breast cancer (labc). | 2000 | 11072788 |
| vasoactive intestinal polypeptide in rat heart atria: the effect of hyperthyroidism. | the effects of transient and sustained hyperthyroidism on vasoactive intestinal polypeptide-like immunoreactivity (vip-li) levels were studied in the heart atria of developing and adult rats. newborn rats were divided into 5 groups. neo-t animals were treated with thyroxine (t4) during postnatal days 1-8 and sacrificed at the age of 60 days. neo-s rats were treated with t4 during postnatal days 1-60 and sacrificed one day later. adult-1 and adult-2 animals received t4 during days 52-60 and were ... | 2000 | 11072802 |
| a novel mutation (m310l) in the thyroid hormone receptor beta causing resistance to thyroid hormone in a brazilian kindred and a neonate. | resistance to thyroid hormone (rth) is an inherited syndrome of reduced tissue responsiveness to thyroid hormone (t3) caused by mutations in the thyroid hormone receptor beta (trbeta). the index patient of the family reported here, a 17-year-old woman, came to medical attention because of a diffuse goiter, short stature, and learning disabilities. biochemical tests revealed an elevated free t4 of 5.2 ng/dl (0.8-2.1), a t3 of 270 ng/dl (80-220), and a nonsuppressed tsh of 1.79 mu/l (0.4-4). admin ... | 2000 | 11073720 |
| virus mutators and antimutators: roles in evolution, pathogenesis and emergence. | virus mutators (mutant alleles that confer a higher mutant-frequency phenotype than that of the wild type) and antimutators (mutant alleles that confer a lower mutant-frequency phenotype) were discovered in bacteriophage t4 over three decades ago, but there is only limited detailed knowledge about such genetic variants in viruses that infect humans and threaten public health. the creation of mutators and antimutators during the course of viral infection (particularly in the case of rna viruses) ... | 2000 | 11074294 |
| thyroid hormones and lipid metabolism in a group of patients over seventy. | numerous studies have suggested a marked correlation between thyroid functionality indices and lipid metabolism. in this trial we assessed the functional parameters of 165 individuals over 70, 87 women and 78 men, correlating the serum values of t3, t4, ft4, tsh with cholesterol, triglycerides, hdl, apo-a and apo-b levels. the correlation was performed over the whole population studied and subsequently, after dividing the population by sex and age (3 age groups: a, 70-75; b, 76-80; c, over 80) i ... | 1999 | 11075619 |
| selective proteolysis of human type 2 deiodinase: a novel ubiquitin-proteasomal mediated mechanism for regulation of hormone activation. | we investigated the mechanism by which t4 regulates its activation to t3 by the type 2 iodothyronine deiodinase (d2). d2 is a short- lived (t1/2 50 min), 31-kda endoplasmic reticulum (er) integral membrane selenoenzyme that generates intracellular t3. inhibition of the ubiquitin (ub) activating enzyme, e1, or mg132, a proteasome blocker, inhibits both the basal and substrate-induced acceleration of d2 degradation. using a catalytically active transiently expressed flag-tagged-nh2-d2, we found ra ... | 2000 | 11075806 |
| postnatal thyroxine supplementation in infants less than 32 weeks' gestation: effects on pulmonary morbidity. | transient hypothyroxinemia in premature newborns has been linked with poor neonatal outcomes. we designed this study to evaluate the effects of early thyroxine (t4) administration in the premature infant. | 2000 | 11076326 |
| induction chemotherapy followed by alternating chemo-radiotherapy in stage iv undifferentiated nasopharyngeal carcinoma. | in locally advanced undifferentiated nasopharyngeal carcinoma (unpc), concomitant chemo-radiotherapy is the only strategy that gave better results over radiation alone in a phase iii trial. adding effective chemotherapy to a concomitant chemo-radiotherapy programme may be a way to improve the results further. 30 patients with previously untreated t4 and/or n2-3 undifferentiated nasopharyngeal carcinoma were consecutively enrolled and initially treated with 3 courses of epidoxorubicin, 90 mg/m2, ... | 2000 | 11076650 |
| changes in cardiac function at rest before and after treatment in primary hypothyroidism. | the aim of the present study was to investigate in hypothyroid patients the reversibility of the prolongation of electromechanical delay and of the impairment of early diastolic relaxation as measured by radionuclide ventriculography after restoration of euthyroidism. we also evaluated doppler echocardiography in relation to scintigraphic findings. twenty-six consecutive hypothyroid patients were studied at diagnosis and after treatment. the time to peak ejection decreased (161 +/- 6 vs 145 +/- ... | 2000 | 11078310 |
| association of hyperthyroidism with serum leptin levels. | the present study was undertaken to determine whether thyroid hormones affect serum leptin levels in patients with hyperthyroidism. in 32 female patients with hyperthyroidism and 30 body mass index (bmi)-matched control subjects, there was no difference in serum leptin concentrations (9.0+/-1.0 v 8.2+/-0.9 microg/l). the serum leptin levels were compared with the expected values for serum leptin derived from the corresponding bmi with the formula for the control subjects (serum leptin [microgram ... | 2000 | 11079817 |
| endoscopic ultrasonography in the preoperative staging of gastric cancer: accuracy and impact on surgical therapy. | endoscopic ultrasonography (eus) is a standard procedure in the preoperative staging of patients with gastric carcinomas. herein we present our experience with eus and discuss the results and their implications for surgical therapy. | 2000 | 11080410 |
| simultaneous determination of plasma ovarian and thyroid hormones during sexual maturation of the hen (gallus domesticus). | the concentrations of ovarian steroids (estradiol--e2, progesterone--p4 and testosterone--t) and thyroid hormones (thyroxine--t4 and triiodothyronine--t3) were determined in blood plasma of the domestic hen during sexual maturation and the initial period of egg lay. blood samples were collected from hy-line pullets at 3 day intervals from days 87 to 144 day of life, i.e. 42 days before and 14 days after the onset of egg lay (oel). ovarian and thyroid hormones were measured by ria methods. during ... | 2000 | 11080911 |
| efficacy of concomitant chemoradiation and surgical salvage for n3 nodal disease associated with upper aerodigestive tract carcinoma. | to determine whether an aggressive approach using trimodality therapy would improve the outcome in head and neck cancer patients with advanced (n3) nodal disease. | 2000 | 11081585 |
| resection of cancer of the tongue base and tonsil via the transhyoid approach. | to assess whether transhyoid resection of the tongue base and tonsil lesions is an effective approach to tumors involving this region compared with more traditional anterior surgical approaches that arbitrarily involve either segmental resection or division of the mandible. | 2000 | 11081588 |
| [amiodarone-induced hyperthyroidism]. | amiodarone is extensively used in cardiology practice because of its excellent antiarrhythmic properties. it produces alterations in thyroid functional because it contains 37% iodine and it is structurally similar to the thyroid hormones. amiodarone inhibits 5'-deiodinase in the liver. the incidence of amiodarone-induced hyperthyroidism is between 6% and 12% of treated patients. the figures for pediatric patients are similar. determination of tri-iodothyronine (t3), thyroxine (t4) and thyroid-st ... | 2000 | 11083993 |
| [positive antithyroglobulin antibodies in patients with differentiated thyroid carcinoma. what significance?]. | the aim of this retrospective study was to determine the predictive value of antithyroglobulin antibodies (tgab) in 238 patients with undetectable thyroglobulin after whole thyroidectomy for differentiated cancer and ablative dose of radioiodine. tgab titers were elevated in 30 patients with undetectable tg level after withdrawal of t4 treatment. in 21 patients, initially positive tgab titers became undetectable without clinical event within 3-84 months after therapy (median 12 months). in 2 cas ... | 2000 | 11084393 |
| effect of hyperthyroidism on insulin-like growth factor-i (igf-i) and igf-binding proteins in adolescent children. | a study was performed on adolescent hyperthyroid patients to determine the effects of hyperthyroidism on insulin-like growth factor (igf)-i and its binding proteins. serum concentrations of immunoreactive total and free igf-i, and igf binding protein (igfbp)-2 and igfbp-3 were determined before and after correction of hyperthyroidism in eight patients with grave's disease and compared to control patients matched for age, sex and pubertal stage. the concentration of serum total igf-i was not sign ... | 2000 | 11085184 |
| subtype specific downregulation of thyroid hormone receptor mrna by beta-adrenoreceptor blockade in the myocardium. | the beta-adrenergic system is very important in cardiovascular medicine. thyroid hormone (t3) affects beta-adrenergic receptors. in cell culture, isoproterenol, a beta-adrenergic agonist, has been shown to upregulate thyroid hormone receptor (tr) mrna, thus indicating a bi-directional regulation. the aim of this study was to evaluate if beta-adrenoreceptor blockade may affect subtype tr mrna expression in vivo. propranolol or vehicle was delivered by implanting an alzet osmotic pump subcutaneous ... | 2000 | 11085356 |
| [an effective case of intraperitoneal and intra-arterial combined chemotherapy for unresectably advanced borrmann type iv gastric carcinoma]. | we report an effective case of intraperitoneal and intra-arterial combined chemotherapy for unresectably advanced borrmann type iv gastric carcinoma. a 59-year-old man was admitted with advanced borrmann type iv gastric carcinoma in june 1999. with informed consent, the patient underwent laparotomy, which revealed far advanced stage iv gastric carcinoma of t4, n3, p1, h0, m1, cy1 for resection. three ports to both subphrenic pouches and douglas' pouch were placed for intraperitoneal infusion che ... | 2000 | 11086465 |
| activation of hypothalamic neuronal nitric oxide synthase in lithium-induced diabetes insipidus rats. | the expression of the neuronal nitric oxide synthase (nnos) gene in the paraventricular (pvn) and supraoptic nuclei (son) in rats with lithium (li)-induced polyuria was examined by using in situ hybridization histochemistry. the state of the thyroid axis in these rats was also examined by in situ hybridization histochemistry for thyrotropin-releasing hormone (trh) and thyroid-stimulating hormone (tsh) mrnas and radioimmunoassay for circulating thyroid hormones. adult male wistar rats consuming a ... | 2001 | 11087959 |
| modeling hiv transfer between dendritic cells and t cells: importance of hiv phenotype, dendritic cell-t cell contact and t-cell activation. | to study the requirements for hiv transfer between dendritic cells (dc) and cd4 t cells, using an in vitro model, combined with flow cytometry. | 2000 | 11089618 |
| analysis of the okazaki fragment distributions along single long dnas replicated by the bacteriophage t4 proteins. | rolling circle replication from m13 dna circles was previously reconstituted in vitro using purified factors encoded by bacteriophage t4. the products are duplex circles with linear tails >100 kb. when t4 dna polymerase deficient in 3' to 5' exonuclease activity was employed, electron microscopy revealed short single-stranded dna "flaps" along the replicated tails. this marked the beginning of each okazaki fragment, allowing an analysis of the lengths of sequential okazaki fragments on individua ... | 2000 | 11090619 |
| serum soluble interleukin-2 receptor levels in patients with breast cancer. | preoperative levels of serum soluble interleukin-2 receptor (1l-2r) were examined in 37 patients with breast cancer. we investigated the correlations of serum soluble il-2r levels with various factors such as stage grouping, lymph node metastasis, distant metastasis, tumor size, histophthological type, estrogen receptor (er), progesterone receptor (pgr) and ca 15-3. serum soluble 1l-2r levels were measured with an enzyme-linked immunosorbent assay. levels of serum soluble 1l-2r in the patients w ... | 1997 | 11091572 |
| genetic analysis of bacteriophage-encoded cochaperonins. | early genetic studies identified the escherichia coli groes and groel genes because mutations in them blocked the growth of bacteriophages lambda and t4. subsequent genetic and biochemical analyses have shown that groes and groel constitute a chaperonin machine, absolutely essential for e. coli growth, because it is needed for the correct folding of many of its proteins. in spite of very little sequence identity to groes, the bacteriophage t4-encoded gp31 protein and the bacteriophage rb49-encod ... | 2000 | 11092834 |
| accelerated growth signals and low tumor-infiltrating lymphocyte levels predict poor outcome in t4 esophageal squamous cell carcinoma. | little is known about the biological nature of t4 esophageal carcinoma growth signals and host defenses. | 2000 | 11093500 |
| renal excretion and saline intake during post-stress immobilization period in rats. | an experiment in which the rats access either to 0.5% or 1.5% saline was designed in order to further characterise the relationship between sodium intake and renal excretion after acute immobilization stress. a saline solution for 3 days was provided to the rats previous to the experimental day. on that day, after finishing acute immobilization stress, all variables under observation were measured every 6 h for 24 h. these periods were denominated as follows: t1 (12.00 to 18.00 h), t2 (18.00 to ... | 2000 | 11094380 |
| revised t3 uptake and t4-to-t3 conversion in brain and cerebellum of 10-day-old rats: a compartment analysis. | the peripheral and cerebral metabolism of thyroid hormones in 10-day-old rats was evaluated by measuring the kinetics of thyroxine (t4) and 3,5,3'-triiodothyronine (t3) fluxes. labeled iodo-compounds were measured in the plasma, cerebellum and brain (without cerebellum) for 24 hours after the intravenous injection of [125i]t4 plus [131i]t3. data were interpreted by compartment analysis. t4 was produced at 8.93 pmol x h(-1) and t3 at 2.26 pmol x h(-1) for 22.7 g body weight. the t4 and t3 distrib ... | 2000 | 11094382 |
| is neuropsychological development related to maternal hypothyroidism or to maternal hypothyroxinemia? | several recent publications have drawn attention to the role of the thyroid hormone status of the mother on the future neuropsychological development of the child. the screening of pregnant women for clinical or subclinical hypothyroidism based on second trimester elevated maternal tsh values has been proposed. here, we have summarized present epidemiological and experimental evidence strongly suggesting that conditions resulting in first trimester hypothyroxinemia (a low for gestational age cir ... | 2000 | 11095417 |
| a characteristic serpin cleavage product of thyroxine-binding globulin appears in sepsis sera. | t4-binding globulin (tbg), the principal thyroid hormone-binding protein of serum, is a member of the serine protease inhibitor (serpin) superfamily. we report a characteristic serpin cleavage product of tbg in sepsis sera. at 49-50 kda, the tbg remnant is 4-5 kda smaller than the intact protein and is the same molecular mass as a tbg cleavage product produced by incubation with polymorphonuclear elastase. incubation with polymorphonuclear leukocytes also produces the 49- to 50-kda remnant, and ... | 2000 | 11095421 |
| persistent increase in bone turnover in graves' patients with subclinical hyperthyroidism. | hyperthyroid patients exhibit accelerated bone loss by increased bone turnover, and normalization of thyroid function is associated with a significant attenuation of increased bone turnover, followed by an increase in bone mineral density. however, of patients with graves' disease (gd) maintained on antithyroid drug (atd) treatment, some exhibit persistent suppression of tsh long after normalization of their serum free t3 (ft3) and free t4 (ft4) levels. the aim of this study was to examine wheth ... | 2000 | 11095447 |
| the effect of droloxifene and estrogen on thyroid function in postmenopausal women. | estrogen is known to increase serum t4-binding globulin (tbg) concentrations, thereby increasing serum total t4 concentrations. serum free t4 concentrations, however, remain normal. tamoxifen, a selective estrogen receptor modifier (serm), also raises serum tbg concentrations, but whether newer serms with less stimulatory action on the endometrium do so is not known. we, therefore, compared the effect of droloxifene, a serm, and conjugated equine estrogen on pituitary-thyroid function in normal ... | 2000 | 11095487 |
| nucleotide-sequence-specific and non-specific interactions of t4 dna polymerase with its own mrna. | the dna-binding dna polymerase (gp43) of phage t4 is also an rna-binding protein that represses translation of its own mrna. previous studies implicated two segments of the untranslated 5'-leader of the mrna in repressor binding, an rna hairpin structure and the adjacent rna to the 3' side, which contains the shine-dalgarno sequence. here, we show by in vitro gp43-rna binding assays that both translated and untranslated segments of the mrna contribute to the high affinity of gp43 to its mrna tar ... | 2000 | 11095675 |
| steroid concentrations in follicular fluid aspirated repeatedly from transitional and cyclic mares. | the objectives of the present study were to determine follicular progesterone (p4) and estradiol-17beta (e2) in transitional mares and to compare follicular steroid concentrations between transitional and cyclic mares. follicles > 8 mm were aspirated under transvaginal ultrasound-guidance 4 times at 3 to 4 day intervals (t1-t4) in norwegian pony mares during vernal transition. during the breeding season, follicular aspirations were conducted in each mare on day 6, day 14 and day 18 after ovulati ... | 2000 | 11097041 |
| [role of lymph nodes in rectal carcinoma]. | the significance of the lymph nodes for the therapy and the prognostics of the rectal cancer are reviewed. not only the depth of penetrations, but the probability of lymph node involvement (in our series tumors staged t1 had in 12% positive lymph nodes, t2 in 20% respectively, t3 in 37% and t4 in 80%) determine the possible surgical treatment. the available data of different techniques in lymphadenectomy and lymph node staging are compared and discussed. the aim of this paper is to give the onco ... | 2000 | 11098582 |
| primary percutaneous approach to upper urinary tract transitional cell carcinoma. | the optimal approach to upper tract tcc remains to be redefined. a routine nephroureterectomy for every filling defect in the upper urinary system, even in the case of a normal contralateral kidney, constitutes an unnecessary mutilation in more than two thirds of the cases. nephroureterectomy does not reduce the need for a long-term cystoscopic follow-up because of the high rate of bladder tumor recurrence that may happen years later after nephroureterectomy. relying solely on radiography and cy ... | 2000 | 11098771 |
| effects of amplification facilitators on diagnostic pcr in the presence of blood, feces, and meat. | the full potential of diagnostic pcr is limited, in part, by the presence of inhibitors in complex biological samples that reduce the amplification efficiency. therefore, different pre-pcr treatments are being used to reduce the effects of pcr inhibitors. the aim of the present study was to investigate the effects of 16 amplification facilitators to enhance dna amplification in the presence of blood, feces, or meat. different concentrations of amplification facilitators and inhibitory samples we ... | 2000 | 11101581 |
| [significance of neoadjuvant chemotherapy for gastric cancer]. | neoadjuvant chemotherapy for high-risk patients with advanced gastric cancer is important to increase the chance for curative resection and make unresectable gastric cancer tumors resectable by down-staging of the tumor. tumors with h0, p0, t3, t4, or n3 are the best candidates for this therapy. randomized controlled phase iii studies are needed in conjunction with accurate staging of the disease by laparoscopy. the results of histopathologic evaluation of resected materials following preoperati ... | 2000 | 11103233 |
| identification of a sea urchin na(+)/k(+)/2cl(-) cotransporter (nkcc): microfilament-dependent surface expression is mediated by hypotonic shock and cyclic amp. | we report the identification of an invertebrate na(+)/k(+)/2cl(-) cotransporter, nkcc. as a model system, we used the immune cells (coelomocytes) of the mediterranean sea urchin paracentrotus lividus. these cells are particularly interesting because they can be activated to undergo a rapid and dynamic change in cell shape. we demonstrate that forskolin, a cyclic amp agonist known to regulate nkcc, induced coelomocyte transformation at doses of 10 micromol l(-)(1) and greater. using two distinct ... | 2001 | 11104718 |
| pseudo-t-even bacteriophage rb49 encodes coco, a cochaperonin for groel, which can substitute for escherichia coli's groes and bacteriophage t4's gp31. | bacteriophage t4-encoded gp31 is a functional ortholog of the escherichia coli groes cochaperonin protein. both of these proteins form transient, productive complexes with the groel chaperonin, required for protein folding and other related functions in the cell. however, gp31 is specifically required, in conjunction with groel, for the correct folding of gp23, the major capsid protein of t4. to better understand the interaction between groel and its cochaperonin cognates, we determined whether ... | 2001 | 11104767 |
| the structure of udp-n-acetylglucosamine 2-epimerase reveals homology to phosphoglycosyl transferases. | bacterial udp-n-acetylglucosamine 2-epimerase catalyzes the reversible epimerization at c-2 of udp-n-acetylglucosamine (udp-glcnac) and thereby provides bacteria with udp-n-acetylmannosamine (udp-mannac), the activated donor of mannac residues. mannac is critical for several processes in bacteria, including formation of the antiphagocytic capsular polysaccharide of pathogens such as streptococcus pneumoniae types 19f and 19a. we have determined the x-ray structure (2.5 a) of udp-glcnac 2-epimera ... | 2000 | 11106477 |
| the role of selenocysteine 133 in catalysis by the human type 2 iodothyronine deiodinase. | human type 2 iodothyronine deiodinase (hd2) catalyzes the activation of t4 to t3. d2, like types 1 and 3 deiodinases, contains selenocysteine (sec) in the highly conserved active center at position 133. to evaluate the contribution of sec133 to the catalytic properties of hd2, we generated mutants in which cysteine (cys) or alanine (ala) replaced sec133. the km (t4) of cys133d2 was 2.1 microm, strikingly higher than that of native d2 (1.4 nm). in contrast, the relative turnover number was 10-fol ... | 2000 | 11108274 |
| [therapy concept in differentiated thyroid gland carcinoma--results of 25 years with 257 patients]. | differentiated thyroid carcinoma is a unique tumour in that a low risk-patient population with a tumour-related death rate of near 0% can be found. yet in these patients the risk and risk factors of curable recurrences must be considered. the question therefore arises, whether in defined subgroups of low risk-patients a reduced extent of treatment (hemithyroidectomy, total thyroidectomy without 131i ablation) may result in cure without recurrence, including low morbidity of treatment and reduced ... | 2000 | 11109916 |
| thyroid function in very-low-birth-weight infants with intraventricular hemorrhage. | the objective of this investigation was to study the natural course of thyroid function in infants with intraventricular hemorrhage (ivh). a cohort of infants < 1,500 grams birth weight, n=247, were included in the analysis. total t4 and thyrotropin from newborn screening during the 1st week of life (test 1) and from repeat screening at 2-4 weeks postnatal age (test 2) were compared in infants with ivh (n=43) and a group of infants without ivh. fifty-nine percent of infants still had transient h ... | 2000 | 11110365 |
| nuclear morphometry for the prediction of regional lymph nodes metastases in patients with cancer of the larynx. | the changes in cell nuclei reflect their activity. quantitative morphometric analyses of tumor nuclei could be instrumental in providing prognostic information. we studied whether, and if so, which specific nuclear parameters and histoclinical factors in patients with cancer of the larynx could be related to the lymph node metastases. specimens were taken from 61 patients surgically treated in the department of otolaryngology, jagiellonian university, cracow, poland, between 1987 and 1988. the p ... | 2000 | 11112977 |
| short-term effects of fiberotomy on relapse of anterior crowding. | the effects of fiberotomy were evaluated in alleviating dental relapse of incisors after orthodontic treatment. the study sample consisted of 23 patients with crowded maxillary and mandibular incisors before orthodontic treatment. the amount of initial crowding was determined according to little's irregularity index. fiberotomy procedures were performed on 11 of the patients 1 week before debonding. the other 12 subjects served as the control group. all patients wore hawley retainers. lateral ce ... | 2000 | 11113795 |
| phylogeny of the major head and tail genes of the wide-ranging t4-type bacteriophages. | we examined a number of bacteriophages with t4-type morphology that propagate in different genera of enterobacteria, aeromonas, burkholderia, and vibrio. most of these phages had a prolate icosahedral head, a contractile tail, and a genome size that was similar to that of t4. a few of them had more elongated heads and larger genomes. all these phages are phylogenetically related, since they each had sequences homologous to the capsid gene (gene 23), tail sheath gene (gene 18), and tail tube gene ... | 2001 | 11114936 |
| k-ras point mutation in the nerve plexuses around the superior mesenteric artery in resectable adenocarcinoma of the pancreatic head: distribution pattern and related factors. | adenocarcinoma of the pancreas is likely to spread into the nerve plexuses around the superior mesenteric artery (sma) at a microscopic level. since there has been no detailed report on how minute cancer invasion is distributed among the peri-sma plexuses or which cases are more vulnerable to such an event, it has long been controversial how to treat this area when resecting the pancreatic head cancer. | 2000 | 11115351 |
| ct or eus for the initial staging of esophageal cancer? a cost minimization analysis. | patients with advanced (t4 and/or m1) esophageal cancer are offered palliative therapy. computed tomography (ct) is sensitive for distant metastases but is less sensitive than endosonography for t4 disease and celiac lymphadenopathy. the aim of this study was to determine whether initial ct or endosonography costs less to diagnose advanced esophageal cancer. | 2000 | 11115901 |
| thyroid hormones and their effects: a new perspective. | the thyroid hormones are very hydrophobic and those that exhibit biological activity are 3',5',3,5-l-tetraiodothyronine (t4), 3',5,3-l-triiodothyronine (t3), 3',5',3-l-triiodothyronine (rt3) and 3,5',-l-diiothyronine (3,5-t2). at physiological ph, dissociation of the phenolic -oh group of these iodothyronines is an important determinant of their physical chemistry that impacts on their biological effects. when non-ionized these iodothyronines are strongly amphipathic. it is proposed that iodothy ... | 2000 | 11117200 |
| successful withdrawal of thyroid hormone therapy in nursing home patients. | studies of community-dwelling patients have indicated that substantial numbers of patients might have had thyroid hormone therapy prescribed inappropriately and that thyroid hormone therapy in some can be discontinued without adverse effects or evidence of clinical hypothyroidism. we wanted to find out whether thyroid hormone therapy in selected nursing home patients could be withdrawn without adverse effect. | 2000 | 11117336 |
| allelic deletions of cell growth regulators during progression of bladder cancer. | cell growth regulators include proteins of the p53 pathway encoded by the genes cdkn2a (p16, p14arf), mdm2, tp53, and cdkn1a (p21) as well as proteins encoded by genes like rb1, e2f, and mycl. in the present study we investigated allelic deletions of all these genes in each recurrent bladder tumor from well-defined clinical material with more than 3 years of follow-up. we followed three groups (22 or 23 patients/group) of patients with: (a) recurrent noninvasive tumors (ta); (b) primary muscle-i ... | 2000 | 11118045 |
| role of the substrate conformation and of the s1 protein in the cleavage efficiency of the t4 endoribonuclease regb. | the t4 endoribonuclease regb is involved in the inactivation of the phage early messengers. it cuts specifically in the middle of ggag sequences found in early messenger intergenic regions but not ggag sequences located in coding sequences or in late messengers. in vitro regb activity is very low but is enhanced by a factor up to 100 by the ribosomal protein s1. in the absence of clear sequence motif distinguishing substrate and non-substrate ggag-containing rnas, we postulated the existence of ... | 2001 | 11118457 |
| docking molecules by families to increase the diversity of hits in database screens: computational strategy and experimental evaluation. | molecular docking programs screen chemical databases for novel ligands that fit protein binding sites. when one compound fits the site well, close analogs typically do the same. therefore, many of the compounds that are found in such screens resemble one another. this reduces the variety and novelty of the compounds suggested. in an attempt to increase the diversity of docking hit lists, the available chemicals directory was grouped into families of related structures. all members of every famil ... | 2001 | 11119652 |
| anemia is associated with decreased local control of surgically treated squamous cell carcinomas of the glottic larynx. | a strong association between hemoglobin levels and tumor control exists in head and neck cancer treated with radiotherapy. this retrospective study has been performed to determine whether or not this association can also be found in the surgical setting. | 2000 | 11121632 |
| which patients with newly diagnosed prostate cancer need a radionuclide bone scan? an analysis based on 631 patients. | although radionuclide bone scans are frequently recommended as part of the staging evaluation for newly diagnosed prostate cancer, most scans are negative for metastases. we hypothesized that gleason score, prostate-specific antigen (psa), and clinical stage could predict for a positive bone scan (bs), and that a low-risk group of patients could be identified in whom bs might be omitted. | 2000 | 11121646 |
| from minichaperone to groel 2: importance of avidity of the multisite ring structure. | structural studies on minichaperones and groel imply a continuous ring of binding sites around the neck of groel. to investigate the importance of this ring, we constructed an artificial heptameric assembly of minichaperones to mimic their arrangement in groel. the heptameric gp31 co-chaperonin from bacteriophage t4, an analogue of groes, was used as a scaffold to display the groel minichaperones. a fusion protein, mc(7), was generated by replacing a part of the highly mobile loop of gp31 (resid ... | 2000 | 11124034 |
| changes in plasma thyroid hormone levels after a single dose of triiodothyronine in premature infants of less than 30 weeks gestational age. | evaluation of thyroid hormone response to a single administration of triiodothyronine (t3) early postnatally to premature infants of <30 weeks gestational age. | 2000 | 11124855 |
| combination cisplatin, 5-fluorouracil and radiation therapy for locally advanced unresectable or medically unfit bladder cancer cases: a southwest oncology group study. | patients with locally advanced bladder cancer or who are not medically fit for surgery are a therapeutic dilemma. radiotherapy with or without single agent cisplatin has been the major therapeutic modality. a phase ii southwest oncology group trial investigated the efficacy and feasibility of 5-fluorouracil, cisplatin and radiation in this patient subset. | 2001 | 11125363 |
| changes in serum thyroxine and thyroid-stimulating hormone concentrations in epileptic dogs receiving phenobarbital for one year. | a multicentric prospective study was conducted to monitor the effect of phenobarbital on serum total thyroxine (t4) and thyroid-stimulating hormone (tsh) concentrations in epileptic dogs. serum t4 concentrations were determined for 22 epileptic dogs prior to initiation of phenobarbital therapy (time 0), and 3 weeks, 6 months, and 12 months after the start of phenobarbital. median t4 concentration was significantly lower at 3 weeks and 6 months compared to time 0. thirty-two percent of dogs had t ... | 2000 | 11126325 |
| reduced ligation during dna base excision repair supported by brca2 mutant cells. | the breast cancer predisposing genes brca1 and brca2 appear to be involved in dna repair. in particular, the sensitivity of brca2-deficient mouse embryonic fibroblasts to ionizing radiation and the demonstrated interaction of the brca2 protein with rad51, a major factor in recombinational repair, indicate that brca2 is important for double strand break repair. the human brca2-deficient human cell line capan-1, whilst being sensitive to ionizing radiation, is also sensitive to the alkylating agen ... | 2000 | 11126365 |
| thyroid hormones regulate hypertrophic chondrocyte differentiation and expression of parathyroid hormone-related peptide and its receptor during endochondral bone formation. | hypothyroidism in children causes developmental abnormalities in bone and growth arrest, while thyrotoxicosis accelerates growth rate and advances bone age. to determine the effects of thyroid hormones on endochondral bone formation, we examined epiphyseal growth plates in control, hypothyroid, thyrotoxic, and hypothyroid-thyroxine (hypo-t4)-treated rats. hypothyroid growth plates were grossly disorganized, contained an abnormal matrix rich in heparan sulfate, and hypertrophic chondrocyte differ ... | 2000 | 11127207 |
| the auditory threshold in a school-age population is related to iodine intake and thyroid function. | the aim of this study was to determine the relationship between auditory capacity and urinary iodine, taking into account thyroid volume and function, in a population of school-age children. audiometry was carried out in 150 children (ages 6-14 years), together with measurements of thyroid volume, thyrotropin (tsh), free t3, free t4, thyroglobulin, antiperoxidase and anti-tsh receptor antibodies, as well as iodine in a casual urine sample. children with a tsh >5 microu/ml were excluded from the ... | 2000 | 11128728 |
| prognostic value of lymph node involvement in oral cancers: a study of 137 cases. | the aim of this study was to assess the prognostic value of lymph node involvement in patients with squamous cell carcinoma of the oral cavity. | 2000 | 11129021 |
| preoperative staging of rectal cancer with mri: accuracy and clinical usefulness. | preoperative staging is essential for planning of optimal therapy for patients with rectal cancer. recently, magnetic resonance imaging (mri) is used frequently because of its benefits of clear pelvic image are better than other diagnostic methods. the purpose of this study was to determine accuracy rates and clinical usefulness of mri in preoperative staging of rectal cancer. | 2000 | 11129420 |
| characterization of thyroid fibrosis in a murine model of granulomatous experimental autoimmune thyroiditis. | this study was initiated to identify and characterize thyroid fibrosis in a murine model of granulomatous experimental autoimmune thyroiditis (g-eat) and determine if tgf-beta1 might be involved in fibrosis. g-eat was induced by transfer of mouse thyroglobulin-sensitized spleen cells activated in vitro with thyroglobulin, anti-il-2r, and il-12. there was almost complete destruction of thyroid follicles, leading to fibrosis of the gland and reduced serum t4 levels. fibrosis was confirmed by stain ... | 2000 | 11129650 |
| myxedema coma: diagnosis and treatment. | myxedema coma, the extreme manifestation of hypothyroidism, is an uncommon but potentially lethal condition. patients with hypothyroidism may exhibit a number of physiologic alterations to compensate for the lack of thyroid hormone. if these homeostatic mechanisms are overwhelmed by factors such as infection, the patient may decompensate into myxedema coma. patients with hypothyroidism typically have a history of fatigue, weight gain, constipation and cold intolerance. physicians should include ... | 2000 | 11130234 |
| clinical evaluation of staging system for nasopharyngeal carcinoma: comparison of fourth and fifth editions of uicc tnm classification. | staging nasopharyngeal carcinoma (npc) by the uicc 4th-edition tnm classification system (the old system) did not give an accurate prognosis because of the uneven distribution of patients in each stage. this system was revised in 1997 (the new system). to evaluate the performance of the new system, 35 patients with npc who had been staged by the old system were restaged according to the new system. restaging of the patients resulted in an overall "downstaging." differences in the overall surviva ... | 2000 | 11130824 |
| in vivo studies of the escherichia coli recb polypeptide lacking its nuclease center. | in vitro, recb1-929, the truncated escherichia coli recb polypeptide, comprising the n-terminal (helicase) domain of recb, can combine with recc and recd subunits of recbcd enzyme. the resulting recb1-929cd heterotrimer is a potent helicase; due to the loss of the nuclease center of recb, it is devoid of dnase activities. by making use of the recb1-929-producing plasmid pmy100, the in vivo behavior of this truncated polypeptide was studied. the following observations were made. (i) large amounts ... | 2000 | 11130867 |
| self-cleavage of p2sp1 rna with mg2+ and non-ionic detergent (brij 58). | the precursor of an rna molecule from t4-infected e. coli cells (p2sp1 rna) has the capacity to cleave itself at specific positions [(upa (139-140) and cpa (170-171)], within a putative loop and stem structure. this sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. we studied the self-cleavage reaction of an rna fragment (guuucguacaaac) (r1) with the sequence corresponding to the p2sp1 rna in the presence of mg2+ and non-ionic detergents. it requires mg2+ ... | 2000 | 11132630 |
| effect of short-term feed restriction and calorie source on hormonal and metabolic responses in geldings receiving a small meal. | the metabolic effects of short-term feed restriction and dietary calorie source were studied in horses receiving high-roughage or high-concentrate diets. four thoroughbred geldings were assigned to four treatment groups in a 4 x 4 latin square experiment. the four treatments were 1) a nutritionally adequate high-roughage ration (70% roughage, 30% concentrate; ahr), 2) a nutritionally adequate high-concentrate ration (40% roughage, 60% concentrate; ahc), 3) 70% of the intake of the ahr diet (rhr) ... | 2000 | 11132825 |
| inactivation of gram-negative bacteria by lysozyme, denatured lysozyme, and lysozyme-derived peptides under high hydrostatic pressure. | we have studied the inactivation of six gram-negative bacteria (escherichia coli, pseudomonas fluorescens, salmonella enterica serovar typhimurium, salmonella enteritidis, shigella sonnei, and shigella flexneri) by high hydrostatic pressure treatment in the presence of hen egg-white lysozyme, partially or completely denatured lysozyme, or a synthetic cationic peptide derived from either hen egg white or coliphage t4 lysozyme. none of these compounds had a bactericidal or bacteriostatic effect on ... | 2001 | 11133464 |
| interacting fidelity defects in the replicative dna polymerase of bacteriophage rb69. | the dna polymerases (gp43s) of the related bacteriophages t4 and rb69 are b family (polymerase alpha class) enzymes that determine the fidelity of phage dna replication. a t4 whose gene 43 has been mutationally inactivated can be replicated by a cognate rb69 gp43 encoded by a recombinant plasmid in t4-infected escherichia coli. we used this phage-plasmid complementation assay to obtain rapid and sensitive measurements of the mutational specificities of mutator derivatives of the rb69 enzyme. rb6 ... | 2001 | 11133987 |
| impact of number of nodes retrieved on outcome in patients with rectal cancer. | we postulated that the pathologic evaluation of the lymph nodes of surgical specimens from patients with rectal cancer can have a substantial impact on time to relapse and survival. | 2001 | 11134208 |
| joining of short dna oligonucleotides with base pair mismatches by t4 dna ligase. | oligonucleotide-directed mutagenesis is a widely used method for studying enzymes and improving their properties. the number of mutants that can be obtained with this method is limited by the number of synthetic 25-30mer oligonucleotides containing the mutation mismatch, becoming impracticably large with increasing size of a mutant library. to make this approach more practical, shorter mismatching oligonucleotides (7-12mer) might be employed. however, the introduction of these oligonucleotides i ... | 2001 | 11134958 |
| perioperative activation of hemostasis in vascular surgery patients. | perioperative activation of hemostasis could play an important role in the occurrence of postoperative cardiac events. the authors conducted a prospective study to assess platelet function, coagulation, and fibrinolysis status during and after infrarenal aortic surgery. | 2001 | 11135724 |
| thyroid disease in hcv carriers undergoing antiviral therapy with interferon plus ribavirin. | high prevalence of thyroid autoantibodies, with or without overt thyroiditis, has been described in hepatitis c patients, particularly in those undergoing alpha-interferon treatment. the aim of this study was to describe the frequency and clinical outcome of thyroid disease in a cohort of hcv carriers before and during combination therapy with interferon plus ribavirin. between may, 1997, and may, 1999, 111 previously untreated (naive) patients were treated with a-interferon plus ribavirin for 6 ... | 2000 | 11136525 |
| autoimmune thyroiditis with severe hypothyroidism resistant to the treatment with high peroral doses of thyroxine: case report. | female patient (42 yr) suffered from autoimmune thyroiditis resulting in severe hypothyroidism. she was treated for several years by district physician with the dose of 150 microg l-thyroxine daily. since the level of tsh was repeatedly very high and no improvement of clinical signs has been observed, she was referred to the medical faculty hospital. thyroid ultrasound showed remarkable diffuse hypoechogenicity, thyroid scintigraphy showed enlarged thyroid with low 99mtc uptake, trh test was nor ... | 2000 | 11137979 |
| gastric tonometer monitoring in infants undergoing repair of coarctation of the aorta. | to evaluate gastric tonometer monitoring for splanchnic hypoperfusion in infants during surgical intervention for aortic coarctation, especially within aortic cross-clamp periods. | 2000 | 11139107 |
| rna-templated dna ligation for transcript analysis. | ligase-mediated gene detection has proven valuable for detection and precise distinction of dna sequence variants. we have recently shown that t4 dna ligase can also be used to distinguish single nucleotide variants of rna sequences. here we describe parameters that influence rna-templated dna ligation by t4 dna ligase. the reaction proceeds much more slowly, requiring more enzyme, compared to ligation of the same oligonucleotides hybridized to the corresponding dna sequence. the reaction is inh ... | 2001 | 11139629 |
| [surgical treatment of pancreatic carcinoma. our experience]. | currently, pancreatic exocrine carcinoma presents high mortality, poor survival after curative surgery and poor resectability rates at the time of diagnosis. the factors which mostly influence prognosis and therapeutic management (curative surgery or palliative treatments) of the patient affected by pancreatic carcinoma, particularly the peroperative stage, are analyzed and discussed in this article. | 2000 | 11140104 |
| [short-term hypofractionated radiotherapy followed by total mesorectal excision]. | is preoperative short-term radiotherapy of operable rectal carcinoma feasible with regard to early side effects and perioperative complications? | 2000 | 11140149 |
| osteoporosis of the spine: correlation between vertebral deformity and bone mineral density in postmenopausal women. | the purpose of this study is to evaluate the relation of bone mineral density (bmd) or the age-matched z score and osteoporotic vertebral fractures. the radiographs and spinal bone mineral density of 131 consecutive postmenopausal patients were retrospectively reviewed. the vertebral fractures between t4 and l4 were graded. sixty-two patients had no fractures, and 69 had 1 or more fractures. in the nonfracture group, the mean age was 62.6 years, bmd was 0.88 g/cm2, and the z score was -1.00. in ... | 2000 | 11140350 |
| herpes zoster in breast cancer patients after radiotherapy. | we have studied the incidence of herpes zoster in patients with adjuvant radiotherapy for breast cancer with special emphasis on possible correlations with other prognostic factors or survival. | 2000 | 11143525 |
| characterization of a novel vibrio parahaemolyticus phage, kvp241, and its relatives frequently isolated from seawater. | a vibriophage, kvp241, and six of its relatives were isolated independently from seawater using vibrio parahaemolyticus as the host. all of the phages had the same morphology (a hexagonal head and a tail with a contractile sheath) and the same host range (specific for some v. parahaemolyticus strains). dna-dna hybridization experiments elucidated that their genomes are highly homologous to each other. analyses of amino acid sequences of putative major capsid proteins indicated that kvp241 may be ... | 2000 | 11145278 |
| linkage between the hormone binding site and the reactive center loop of thyroxine binding globulin. | thyroxine binding globulin (tbg) is the major carrier of the thyroid hormones triiodothyronine (t3) and thyroxine (t4) in plasma. tbg is member of the serpin family of proteins although it has no proteinase inhibitory activity. in this study we show that tbg has properties typical of a metastable serpin and provide evidence that occupancy of the hormone binding site alters the conformation of the reactive center loop. after reactive center loop cleavage by endoproteinase asp-n or neutrophil elas ... | 2000 | 11147833 |
| unwinding of unnatural substrates by a dna helicase. | helicases separate double-stranded dna into single-stranded dna intermediates that are required during replication and recombination. these enzymes are believed to transduce free energy available from atpase activity to unwind the duplex and translocate along the nucleic acid lattice. the nature of enzyme-substrate interactions between helicases and duplex dna substrates has not been well-defined. most helicases require a single-stranded dna overhang adjacent to duplex dna in order to initiate u ... | 2001 | 11148049 |
| the reaction mechanism of dna glycosylase/ap lyases at abasic sites. | dna glycosylase and glycosylase/abasic (ap) lyases are the enzymes responsible for initiating the base excision repair pathway by recognizing the damaged target base and catalyzing the breakage of the base-sugar glycosyl bond. the subset of glycosylases that have an associated ap lyase activity also catalyze dna strand breakage at the resulting or preexisting ap site via a beta-elimination reaction, proceeding from an enzyme-dna imino intermediate. two distinct mechanisms have been proposed for ... | 2001 | 11148051 |
| combined resection of the involved organs in t4 gastric cancer. | it remains controversial whether extended surgeries for gastric cancer can improve the postoperative survival rate. this study was designed to evaluate the effectiveness of a combined resection of the involved organs for improving the survival of gastric cancer patients. | 2000 | 11149053 |