Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a multiplex oligonucleotide ligation-pcr as a complementary tool for subtyping of salmonella typhimurium. | subtyping below the serovar level is essential for surveillance and outbreak detection and investigation of salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) and its monophasic variant 1,4,[5],12:i:- (s. 1,4,[5],12:i:-), frequent causes of foodborne infections. in an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation-pcr (mol-pcr) assay was developed which combines different types of molecular markers ... | 2015 | 26205523 |
| vasoactive intestinal peptide (vip) differentially affects inflammatory immune responses in human monocytes infected with viable salmonella or stimulated with lps. | we compared the effect of vip on human blood monocytes infected with salmonella typhimurium 4/74 or stimulated with lps. vip (10(-7)m) increased monocyte viability by 24% and 9% when cultured for 24h with 4/74 or salmonella lps (100ng/ml), respectively. significantly increased (p<0.05) numbers of 4/74 were also recovered from monocytes co-cultured with vip after 6h post-infection (pi) and this remained high after 24h pi. both 4/74 and lps increased (p<0.05) the concentration of tnf-α, il-1β and ... | 2015 | 26206287 |
| effect of dietary nonphytate phosphorus content on ileal lymphocyte subpopulations and cytokine expression in the cecal tonsils and spleen of laying hens that were or were not orally inoculated with salmonella typhimurium. | to evaluate the effects of dietary nonphytate phosphorus (npp) content on ileal lymphocyte subpopulations and cytokine expression in the cecal tonsils and spleen of hens that were or were not inoculated with salmonella typhimurium. | 2015 | 26207969 |
| investigation of nalidixic acid resistance mechanism in salmonella enterica using molecular simulation techniques. | the emergence of nalidixic acid-resistant strains of salmonella typhimurium remains to be a major public health problem. in particular, the substitution of asn in place of asp at the 87 loci in the gyra of s. typhimurium was experimentally stated for nalidixic acid resistance. however, the data on the possible mechanism of nalidixic acid resistance are limited. in this study, i-mutant2.0 and duet program were employed to explore the impact of mutation on the stability of gyra protein. subsequent ... | 2015 | 26208690 |
| exposure of salmonella enterica serovar typhimurium to three humectants used in the food industry induces different osmoadaptation systems. | common salt (nacl) is frequently used by the food industry to add flavor and to act as a humectant in order to reduce the water content of a food product. the improved health awareness of consumers is leading to a demand for food products with reduced salt content; thus, manufacturers require alternative water activity-reducing agents which elicit the same general effects as nacl. two examples include kcl and glycerol. these agents lower the water activity of a food matrix and also contribute to ... | 2015 | 26209672 |
| salmonella occurrence and enterobacteriaceae counts in pig feed ingredients and compound feed from feed mills in ireland. | the purpose of this study was to assess the occurrence of non-typhoidal salmonellae and enterobacteriaceae counts in raw ingredients and compound feeds sampled from feed mills manufacturing pig diets. between november 2012 and september 2013, feed ingredients (n=340) and compound pig feed (n=313) samples were collected from five commercial feed mills and one home compounder at various locations throughout ireland. feed ingredients included cereals, vegetable protein sources and by-products of oi ... | 2015 | 26211839 |
| [outbreak of salmonella typhimurium infections associated with consumption of chorizo in bizkaia]. | a report is presented on an outbreak of salmonella enterica serovar typhimurium infection that affected six people. the epidemiological and laboratory investigation associated the outbreak with the consumption of homemade chorizo purchased at a local street market. the vendor and producer were informed that the sale of meat products without sanitary authorization is prohibited, and the product was withdrawn from sale. | 2016 | 26211840 |
| polyphenols rich fraction from geoffroea decorticans fruits flour affects key enzymes involved in metabolic syndrome, oxidative stress and inflammatory process. | geoffroea decorticans (chañar), is widely distributed throughout northwestern argentina. its fruit is consumed as flour, arrope or hydroalcoholic beverage. the chañar fruits flour was obtained and 39 phenolic compounds were tentatively identified by hplc-ms/ms(n). the compounds comprised caffeic acid glycosides, simple phenolics (protocatechuic acid and vanillic acid), a glycoside of vanillic acid, p-coumaric acid and its phenethyl ester as well as free and glycosylated flavonoids. the polypheno ... | 2016 | 26212988 |
| a tpl2 (map3k8) disease-risk polymorphism increases tpl2 expression thereby leading to increased pattern recognition receptor-initiated caspase-1 and caspase-8 activation, signalling and cytokine secretion. | ibd is characterised by dysregulated intestinal immune homeostasis and cytokine secretion. in the intestine, properly regulating pattern recognition receptor (prr)-mediated signalling and cytokines is crucial given the ongoing host-microbial interactions. tpl2 (map3k8, cot) contributes to prr-initiated pathways, yet the mechanisms for tpl2 signalling contributions in primary human myeloid cells are incompletely understood and its role in intestinal myeloid cells is poorly defined. furthermore, f ... | 2016 | 26215868 |
| in vitro synergistic effect of the cm11 antimicrobial peptide in combination with common antibiotics against clinical isolates of six species of multidrug-resistant pathogenic bacteria. | during the last decades, increase of antibiotic resistance among pathogenic bacteria has been considered as a global concern. therefore, it is important to find new antimicrobial agents and/or therapeutic strategies. in previous studies we investigated antibacterial activity of the cm11 peptide against multiple drug resistant clinical isolates of six bacteria species including pseudomonas aeruginosa, staphylococcus aureus, acinetobacter baumannii, escherichia coli, klebsiella pneumoniae and salm ... | 2015 | 26216264 |
| distribution and molecular characterization of salmonella enterica hypermutators in retail food in china. | hypermutable pathogens can easily acquire mutation opportunities, as well as antimicrobial resistance, and are tremendous hazards to food safety and public health. in this study, a total of 96 (7.6%) hypermutators were identified from 1,264 salmonella isolates recovered from retail foods. pulsed-field gel electrophoresis analysis indicated that hypermutators were genetically diverse. amino acid substitution of val421phe was detected in muts in one hypermutator and val246ala in 56 other hypermuta ... | 2015 | 26219361 |
| intestinal inflammation leads to a long-lasting increase in resistance to systemic salmonellosis that requires macrophages but not b or t lymphocytes at the time of pathogen challenge. | intestinal inflammation is associated with systemic translocation of commensal antigens and the consequent activation of b and t lymphocytes. the long-term consequences of such immune activation are not completely understood. | 2015 | 26222341 |
| methionine sulfoxide reductase a (msra) contributes to salmonella typhimurium survival against oxidative attack of neutrophils. | salmonella typhimurium (st) must evade neutrophil assault for infection establishment in the host. myeloperoxidase generated hocl is the key antimicrobial agent produced by the neutrophils; and methionine (met) residues are the primary targets of this oxidant. oxidation of mets leads to methionine sulfoxide (met-so) formation and consequently compromises the protein function(s). methionine sulfoxide reductase a (msra) reductively repairs met-so to mets. in this manner, msra maintains the functio ... | 2015 | 26224245 |
| immunological study of an attenuated salmonella typhimurium expressing apxia, apxiia, apxiiia and ompa of actinobacillus pleuropneumoniae in a mouse model. | salmonella typhimurium strain expressing the actinobacillus pleuropneumoniae antigens, apxia, apxiia, apxiiia and ompa, was previously constructed as a vaccine candidate for porcine pleuropneumonia. this strain was a live attenuated (∆lon∆cpxr∆asd)salmonella as a delivery host and contained a vector containing asd. an immunological study of lymphocyte proliferation, t-lymphocyte subsets and cytokines in the splenocytes of a mouse model was carried out after stimulation with the candidate salmone ... | 2016 | 26227587 |
| proteomics evidence for the activity of the putative antibacterial plant alkaloid (-)-roemerine: mainstreaming omics-guided drug discovery. | discovery of new antibacterials with novel mechanisms is important to counteract the ingenious resistance mechanisms of bacteria. in this connection, omics-guided drug discovery offers a rigorous method in the quest of new antibacterials. (-)-roemerine is a plant alkaloid that has been reported to possess putative antibacterial activity against escherichia coli, bacillus subtilis, and salmonella typhimurium. the aim of the present study was to characterize the activity of (-)-roemerine in escher ... | 2015 | 26230533 |
| regulation and function of the salmonella mgtc virulence protein. | salmonella enterica serovar typhimurium produces many virulence proteins to cause diseases. the salmonella mgtc protein is one of such virulence proteins specially required for intracellular proliferation inside macrophages and mouse virulence. in this review, we will cover how the mgtc gene is turned on or off and what the signals required for mgtc expression are. later in this review, we will discuss a recent understanding of mgtc function in salmonella pathogenesis by identifying its target p ... | 2015 | 26231375 |
| monitoring the incidence and causes of diseases potentially transmitted by food in australia: annual report of the ozfoodnet network, 2011. | this report summarises the incidence of diseases potentially transmitted by food in australia and details outbreaks associated with food in 2011. ozfoodnet sites reported 30,957 notifications of 9 diseases or conditions that may be transmitted by food. the most commonly notified infections were campylobacter (17,733 notifications) followed by salmonella (12,271 notifications). the most frequently notified salmonella serotype was salmonella typhimurium, accounting for 48% of all salmonella notifi ... | 2015 | 26234259 |
| tailored delivery of analgesic ziconotide across a blood brain barrier model using viral nanocontainers. | the blood brain barrier (bbb) is often an insurmountable obstacle for a large number of candidate drugs, including peptides, antibiotics, and chemotherapeutic agents. devising an adroit delivery method to cross the bbb is essential to unlocking widespread application of peptide therapeutics. presented here is an engineered nanocontainer for delivering peptidic drugs across the bbb encapsulating the analgesic marine snail peptide ziconotide (prialt®). we developed a bi-functional viral nanocontai ... | 2015 | 26234920 |
| tumor-targeting salmonella typhimurium a1-r arrests a chemo-resistant patient soft-tissue sarcoma in nude mice. | a patient-derived nude-mouse model of soft-tissue sarcoma has been established and treated in the following groups: (1) untreated controls; (2) gemcitabine (gem) (80 mg/kg, ip, weekly, 3 weeks); (3) pazopanib (100 mg/kg, orally, daily, 3 weeks) and (4) salmonella typhimurium a1-r (5 × 10(7) cfu/body, ip, weekly, 3 weeks). the sarcoma was resistant to gem (p = 0.879). pazopanib tended to reduce the tumor volume compared to the untreated mice, but there was no significant difference (p = 0.115). s ... | 2015 | 26237416 |
| gold nanoparticles enhanced sers aptasensor for the simultaneous detection of salmonella typhimurium and staphylococcus aureus. | salmonella typhimurium and staphylococcus aureus are most common causes of food-associated disease. a raman based biosensor was developed for s. typhimurium and s. aureus detection simultaneously. the biosensor was based on nanoparticles enhanced raman intensity and the specific recognition of aptamer. the raman signal probe and the capture probe are built. gold nanoparticles (gnps) modified with raman molecules (mercaptobenzoic acid and 5,5'-dithiobis(2-nitrobenzoic acid)) and aptamer are used ... | 2015 | 26241735 |
| structures of substrate- and nucleotide-bound propionate kinase from salmonella typhimurium: substrate specificity and phosphate-transfer mechanism. | kinases are ubiquitous enzymes that are pivotal to many biochemical processes. there are contrasting views on the phosphoryl-transfer mechanism in propionate kinase, an enzyme that reversibly transfers a phosphoryl group from propionyl phosphate to adp in the final step of non-oxidative catabolism of l-threonine to propionate. here, x-ray crystal structures of propionate- and nucleotide-bound salmonella typhimurium propionate kinase are reported at 1.8-2.0 å resolution. although the mode of nucl ... | 2015 | 26249345 |
| salmonella typhimurium strain expressing oprf-opri protects mice against fatal infection by pseudomonas aeruginosa. | pseudomonas aeruginosa poses a major threat to human health and to the mink industry. thus, development of vaccines that elicit robust humoral and cellular immunity against p. aeruginosa is greatly needed. in this study, a recombinant attenuated salmonella vaccine (rasv) that expresses the outer membrane proteins fusion oprf190-342 -opri21-83 (f1i2) from p. aeruginosa was constructed and the potency of this vaccine candidate assessed by measuring f1i2-specific humoral immune responses upon vacci ... | 2015 | 26249788 |
| heat-killed lactobacillus spp. cells enhance survivals of caenorhabditis elegans against salmonella and yersinia infections. | this study examined the effect of feeding heat-killed lactobacillus cells on the survival of caenorhabditis elegans nematodes after salmonella typhimurium and yersinia enterocolitica infection. the feeding of heat-killed lactobacillus plantarum 133 (lp133) and lactobacillus fermentum 21 (lp21) cells to nematodes was shown to significantly increase the survival rate as well as stimulate the expression of pmk-1 gene that key factor for c. elegans immunity upon infection compared with control nemat ... | 2015 | 26250615 |
| streptomycin induced stress response in salmonella enterica serovar typhimurium shows distinct colony scatter signature. | we investigated the streptomycin-induced stress response in salmonella enterica serovars with a laser optical sensor, bardot (bacterial rapid detection using optical scattering technology). initially, the top 20 s. enterica serovars were screened for their response to streptomycin at 100 μg/ml. all, but four s. enterica serovars were resistant to streptomycin. the mic of streptomycin-sensitive serovars (enteritidis, muenchen, mississippi, and schwarzengrund) varied from 12.5 to 50 μg/ml, while s ... | 2015 | 26252374 |
| correction: correction: signatures of adaptation in human invasive salmonella typhimurium st313 populations from sub-saharan africa. | 2015 | 26252636 | |
| use of caprylic acid to control pathogens (escherichia coli o157:h7 and salmonella enterica serovar typhimurium) in apple juice at mild heat temperature. | the aim of this study was to examine the effects of caprylic acid (ca) on pathogens in apple juice having intrinsic organic acids, and to determine any synergistic effects. | 2015 | 26257401 |
| biofilm formation ability of salmonella enterica serovar typhimurium acrab mutants. | recent studies offer contradictory findings about the role of multidrug efflux pumps in bacterial biofilm development. thus, the aim of this study was to investigate the involvement of the acrab efflux pump in biofilm formation by investigating the ability of acrb and acrab null mutants of salmonella enterica serovar typhimurium to produce biofilms. three models were used to compare the ability of s. typhimurium wild-type and its mutants to form biofilms: formation of biofilm on polystyrene surf ... | 2015 | 26260191 |
| inactivated salmonella enterica serovar typhimurium monophasic variant (s. typhimurium 1,4,[5],12:i-) in sows is effective to control infection in piglets under field condition. | the monophasic variant of salmonella enterica serovar typhimurium, namely salmonella 1,4,[5],12:i-, has been increasingly responsible for foodborne human cases of disease and is most frequently detected in pork, since the variant is widely spread in pig farms. the aim of this study was to assess the efficacy of an autologous vaccine in decreasing the prevalence of salmonella 1,4,[5],12:i-, in pigs. the trial was performed in a multisite pig production system of northern italy. the autogenous vac ... | 2015 | 26260858 |
| ulleungamides a and b, modified α,β-dehydropipecolic acid containing cyclic depsipeptides from streptomyces sp. kcb13f003. | two novel cyclic depsipeptides, ulleungamides a (1) and b (2), were isolated from cultures of terrestrial streptomyces sp. their structures were determined by analyses of spectroscopic data and various chemical transformations, including modified mosher's method, advanced marfey's method, pgme, gitc derivatizations, and snatzke's method. ulleungamides were determined to be a new class of peptides bearing unprecedented units, such as 5-hydroxy-6-methyl-2,3-dehydropipecolic acid, 4,5-dihydroxy-6-m ... | 2015 | 26262430 |
| symmetry controlled, genetic presentation of bioactive proteins on the p22 virus-like particle using an external decoration protein. | viruses use spatial control of constituent proteins as a means of manipulating and evading host immune systems. similarly, precise spatial control of proteins encapsulated or presented on designed nanoparticles has the potential to biomimetically amplify or shield biological interactions. previously, we have shown the ability to encapsulate a wide range of guest proteins within the virus-like particle (vlp) from salmonella typhimurium bacteriophage p22, including antigenic proteins from human pa ... | 2015 | 26266824 |
| characterization of novel factors involved in swimming and swarming motility in salmonella enterica serovar typhimurium. | salmonella enterica utilizes flagellar motility to swim through liquid environments and on surfaces. the biosynthesis of the flagellum is regulated on various levels, including transcriptional and posttranscriptional mechanisms. here, we investigated the motility phenotype of 24 selected single gene deletions that were previously described to display swimming and swarming motility effects. mutations in flge, flih, ydiv, rfag, yjcc, stm1267 and stm3363 showed an altered motility phenotype. deleti ... | 2015 | 26267246 |
| activity-based proteomic profiling of deubiquitinating enzymes in salmonella-infected macrophages leads to identification of putative function of uch-l5 in inflammasome regulation. | although protein ubiquitination has been shown to regulate multiple processes during host response to salmonella enterica serovar typhimurium infection, specific functions of host deubiquitinating enzymes remain unknown in this bacterial infection. by using chemical proteomics approach, in which deubiquitinating enzymes were labeled by an active-site probe and analyzed by quantitative proteomics, we identified novel deubiquitinases in chicken macrophages based on their reactivity with the probe. ... | 2015 | 26267804 |
| genotoxicity evaluation of oryeong-san water extract using in vitro and in vivo tests. | oryeong-san, a mixture of five herbal plants, is a well-known therapy for renal-associated diseases such as those manifesting edema, dysuria, and oliguria. | 2015 | 26268991 |
| effect of rosemary essential oil and modified-atmosphere packaging (map) on meat quality and survival of pathogens in poultry fillets. | the effect of rosemary (rosmarinus officinalis l.) essential oil (reo) and modified-atmosphere packaging (map) on the survival of certain pathogens (salmonella typhimurium and listeria monocytogenes) in poultry fillets and on their meat quality during 7 days of refrigerated storage were investigated. because reo at 0.05% and 0.1% had weak antibacterial activity and reo at 0.3%, 0.5% and 1.0% imparted unacceptable organoleptic properties, only reo at 0.2% was used to treat the poultry meat. the r ... | 2015 | 26273279 |
| quantification of cytosolic vs. vacuolar salmonella in primary macrophages by differential permeabilization. | intracellular bacterial pathogens can replicate in the cytosol or in specialized pathogen-containing vacuoles (pcvs). to reach the cytosol, bacteria like shigella flexneri and francisella novicida need to induce the rupture of the phagosome. in contrast, salmonella typhimurium replicates in a vacuolar compartment, known as salmonella-containing vacuole (scv). however certain mutants of salmonella fail to maintain scv integrity and are thus released into the cytosol. the percentage of cytosolic v ... | 2015 | 26274778 |
| prazosin-conjugated matrices based on biodegradable polymers and α-amino acids--synthesis, characterization, and in vitro release study. | novel and promising macromolecular conjugates of the α1-adrenergic blocker prazosin were directly synthesized by covalent incorporation of the drug to matrices composed of biodegradable polymers and α-amino acids for the development of a polymeric implantable drug delivery carrier. the cyto- and genotoxicity of the synthesized matrices were evaluated using a bacterial luminescence test, protozoan assay, and salmonella typhimurium ta1535. a new urethane bond was formed between the hydroxyl end-gr ... | 2015 | 26274943 |
| non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium. | the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ... | 2015 | 26280244 |
| antibacterial effects and action modes of asiatic acid. | in this study, the antibacterial effects and action modes of asiatic acid against the foodborne bacterial pathogens escherichia coli o157:h7, salmonella typhimurium dt104, pseudomonas aeruginosa, listeria monocytogenes, staphylococcus aureus, enterococcus faecalis, and bacillus cereus were examined. minimal inhibitory concentrations (mics) of asiatic acid against these bacteria were in the range of 20-40 μg/ml. minimum bactericidal concentrations of asiatic acid were in the range of 32-52 μg/ml. ... | 2015 | 26280399 |
| toxic potentials of ten herbs commonly used for aphrodisiac effect in turkey. | sexual dysfunction is a serious problem worldwide. in turkey, herbal products are used by some people suffering from sexual dysfunction. despite their therapeutic advantages, some constituents of herbs are potentially toxic and pose health risks because they can be bought from the market without a prescription. therefore, we aimed to determine the safety of herbs possessing aphrodisiac effects, chosen on the basis of their frequency of medicinal use and commercial importance in turkey. | 2015 | 26281312 |
| mdsabc-mediated pathway for pathogenicity in salmonella enterica serovar typhimurium. | mdsabc is a salmonella-specific tripartite efflux pump that has been implicated in the virulence of salmonella enterica serovar typhimurium; however, little is known about the virulence factors associated with this pump. we observed mdsabc expression-dependent alterations in the degree of resistance to extracellular oxidative stress and macrophage-mediated killing. thin-layer chromatography and tandem mass spectrometry analyses revealed that overexpression of mdsabc led to increased secretion of ... | 2015 | 26283336 |
| hha has a defined regulatory role that is not dependent upon h-ns or stpa. | the hha family of proteins is involved in the regulation of gene expression in enterobacteria by forming complexes with h-ns-like proteins. whereas several amino acid residues of both proteins participate in the interaction, some of them play a key role. residue d48 of hha protein is essential for the interaction with h-ns, thus the d48n substitution in hha protein abrogates h-ns/hha interaction. despite being a paralog of h-ns protein, stpa interacts with hhad48n with higher affinity than with ... | 2015 | 26284052 |
| vasculature disruption enhances bacterial targeting of autochthonous tumors. | attenuated salmonella enterica serovar typhimurium (s. typhimurium) has been developed as a vector to deliver therapeutic agents to tumors. the potential of s. typhimurium in cancer therapy is largely due to its reported propensity to accumulate at greater than 1,000-fold higher concentrations in tumors relative to healthy tissues. in this study, we compared bacterial colonization of tumors in a subcutaneous transplantation model with a more clinically relevant autochthonous tumor model. followi ... | 2015 | 26284135 |
| an avian influenza a (h7n9) virus vaccine candidate based on the fusion protein of hemagglutinin globular head and salmonella typhimurium flagellin. | a novel influenza virus, subtype h7n9, circulated through china in 2013-2014. its higher rates of human infection in a wide range of locations within china and the associated increased likelihood of human-to-human transmission have caused global concern. recombinant subunit vaccines provide safe and targeted protection against viral infections. however, the protective efficacy of recombinant subunit vaccines tends to be less potent than vaccines made from whole viruses. studies have shown that b ... | 2015 | 26286143 |
| genetically modified "obligate" anaerobic salmonella typhimurium as a therapeutic strategy for neuroblastoma. | neuroblastoma currently has poor prognosis, therefore we proposed a new strategy by targeting neuroblastoma with genetically engineered anaerobic salmonella (sal-yb1). | 2015 | 26286454 |
| antioxidant, mutagenic, and antimutagenic activities of tragopogon longirostis var. longirostis, an edible wild plant in turkey. | the ethanolic extract of tragopogon longirostis var. longirostis, a wild edible plant in anatolia was isolated, and its antioxidant, mutagenic, and antimutagenic properties were investigated. | 2017 | 26288475 |
| salmonella induces pd-l1 expression in b cells. | salmonella persists for a long time in b cells; however, the mechanism(s) through which infected b cells avoid effector cd8 t cell responses has not been characterized. in this study, we show that salmonella infects and survives within all b1 and b2 cell subpopulations. b cells are infected with a salmonella typhimurium strain expressing an ovalbumin (ova) peptide (siinfekl) to evaluate whether b cells process and present salmonella antigens in the context of mhc-i molecules. our data showed tha ... | 2015 | 26292028 |
| biofilm formation and genetic diversity of salmonella isolates recovered from clinical, food, poultry and environmental sources. | in the present study, salmonella isolates (n=40) recovered from clinical, food, poultry and environmental sources were characterized for serotype identification, genetic diversity and biofilm formation capability. serotype identification using multiplex pcr assay revealed six isolates to be salmonella typhimurium, 14 as salmonella enteritidis, 11 as salmonella typhi, and the remaining nine isolates unidentified were considered as other salmonella spp. most of the salmonella isolates (85%) produc ... | 2015 | 26292170 |
| protection of neonatal broiler chickens following in ovo delivery of oligodeoxynucleotides containing cpg motifs (cpg-odn) formulated with carbon nanotubes or liposomes. | unformulated oligodeoxynucleotides (odn) containing cpg motifs (cpg-odn) have been shown to stimulate the innate immune system against a variety of bacterial, viral, and protozoan infections in a variety of vertebrate species. we have previously shown that in ovo delivery of unformulated cpg-odn was able to significantly protect neonatal broiler chickens against escherichia coli or salmonella typhimurium infections. the objectives of this study were to examine the safety and immunoprotective eff ... | 2015 | 26292531 |
| motility modulation by the small non-coding rna sroc in salmonella typhimurium. | bacterial regulatory networks of gene expression include the interaction of diverse types of molecules such as the small non-coding rnas (srnas) and their cognate messenger rnas (mrnas). in this study, we demonstrated that the salmonella typhimurium srna sroc is significantly expressed between the late-exponential and stationary phase of growth in an rpos-dependent manner. the expression of flagellar genes predicted as targets of this srna was quantitatively analyzed in both a δsroc mutant and a ... | 2015 | 26293911 |
| genetic control of bacterial biofilms. | nearly all bacterial species, including pathogens, have the ability to form biofilms. biofilms are defined as structured ecosystems in which microbes are attached to surfaces and embedded in a matrix composed of polysaccharides, edna, and proteins, and their development is a multistep process. bacterial biofilms constitute a large medical problem due to their extremely high resistance to various types of therapeutics, including conventional antibiotics. several environmental and genetic signals ... | 2016 | 26294280 |
| draft genome sequences of 19 salmonella enterica serovar typhimurium [4,5:i:-] strains resistant to nalidixic acid from a long-term outbreak in italy. | here, we present the draft genome sequences of 19 salmonella enterica serovar typhimurium monophasic variant [4,5:i:-] strains involved in a long-term salmonellosis outbreak that occurred in central italy in 2013 to 2014. | 2015 | 26294627 |
| construction of an oral vaccine for transmissible gastroenteritis virus based on the tgev n gene expressed in an attenuated salmonella typhimurium vector. | this research aimed to develop an oral vaccine for transmissible gastroenteritis virus (tgev) based on the tgev n gene expressed in an attenuated salmonella typhimurium vector and aimed to evaluate the vaccine's immune response in piglets. recombinant plasmid pvax-n was transformed into competent cells of attenuated s. typhimurium sl7207 via electroporation. after it was identified via rt-pcr and double digestion, the screened recombinant bacteria presenting pvax-n were named sl7207 (pvax-n). to ... | 2016 | 26297958 |
| assessment by ames test and comet assay of toxicity potential of polymer used to develop field-capable rapid-detection device to analyze environmental samples. | there is need for devices that decrease detection time of food-borne pathogens from days to real-time. in this study, a rapid-detection device is being developed and assessed for potential cytotoxicity. the device is comprised of melt-spun polypropylene coupons coated via oxidative chemical vapor deposition (ocvd) with 3,4-ethylenedioxythiophene (edot), for conductivity and 3-thiopheneethanol (3te), allowing antibody attachment. the ames test and comet assay have been used in this study to exami ... | 2017 | 26301164 |
| structure-based design of inhibitors of the crucial cysteine biosynthetic pathway enzyme o-acetyl serine sulfhydrylase. | the cysteine biosynthetic pathway is of fundamental importance for the growth, survival, and pathogenicity of the many pathogens. this pathway is present in many species but is absent in mammals. the ability of pathogens to counteract the oxidative defences of a host is critical for the survival of these pathogens during their long latent phases, especially in anaerobic pathogens such as entamoeba histolytica, leishmania donovani, trichomonas vaginalis, and salmonella typhimurium. all of these o ... | 2016 | 26303427 |
| genotypic diversity, pathogenic potential and the resistance profile of salmonella typhimurium strains isolated from humans and food from 1983 to 2013 in brazil. | salmonella enterica subsp. enterica serovar typhimurium is one of the leading serovars that causes salmonellosis worldwide. however, few studies have molecularly characterized s. typhimurium strains in brazil. in this study, we genotyped 92 s. typhimurium strains isolated from humans (43) and food (49) between 1983 and 2013 in brazil using pfge, multiple-locus variable number of tandem repeats analysis (mlva) and enterobacterial repetitive intergenic consensus pcr (eric-pcr). moreover, we assess ... | 2015 | 26307078 |
| [structure and function of the bacterial flagellar type iii protein export system in salmonella ]. | the bacterial flagellum is a filamentous organelle that propels the bacterial cell body in liquid media. for construction of the bacterial flagellum beyond the cytoplasmic membrane, flagellar component proteins are transported by its specific protein export apparatus from the cytoplasm to the distal end of the growing flagellar structure. the flagellar export apparatus consists of a transmembrane export gate complex and a cytoplasmic atpase ring complex. flagellar substrate-specific chaperones b ... | 2015 | 26310179 |
| genomic variability of serial human isolates of salmonella enterica serovar typhimurium associated with prolonged carriage. | salmonella enterica serovar typhimurium is an important foodborne human pathogen that often causes self-limiting but severe gastroenteritis. prolonged excretion of s. typhimurium after the infection can lead to secondary transmissions. however, little is known about within-host genomic variation in bacteria associated with asymptomatic shedding. genomes of 35 longitudinal isolates of s. typhimurium recovered from 11 patients (children and adults) with culture-confirmed gastroenteritis were seque ... | 2015 | 26311853 |
| centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection. | we present a centrifugal microfluidic device which enables multiplex foodborne pathogen identification by loop-mediated isothermal amplification (lamp) and colorimetric detection using eriochrome black t (ebt). five identical structures were designed in the centrifugal microfluidic system to perform the genetic analysis of 25 pathogen samples in a high-throughput manner. the sequential loading and aliquoting of the lamp cocktail, the primer mixtures, and the dna sample solutions were accomplishe ... | 2016 | 26322592 |
| insights into stabilizing interactions in the distorted domain-swapped dimer of salmonella typhimurium survival protein. | the survival protein sure from salmonella typhimurium (stsure) is a dimeric protein that functions as a phosphatase. sure dimers are formed by the swapping of a loop with a pair of β-strands and a c-terminal helix between two protomers. in a previous study, the asp230 and his234 residues were mutated to ala to abolish a hydrogen bond that was thought to be crucial for c-terminal helix swapping. these mutations led to functionally inactive and distorted dimers in which the two protomers were rela ... | 2015 | 26327371 |
| seasonal fluctuations in air pollution in dazaifu, japan, and effect of long-range transport from mainland east asia. | to clarify the seasonal fluctuations in air pollution and the effect of long-range transport, we collected airborne particles (n=118) at dazaifu in fukuoka, japan, from june 2012 to may 2013 and measured pb and so4(2-), which are indicators of the long-range transport of anthropogenic air pollutants, as well as their mutagenicity, and other factors. the levels of airborne particles, pb, and so4(2-) were very high on march 4, 8, 9, and 19, and may 13, 21, and 22, 2013. the backward trajectories i ... | 2015 | 26328496 |
| molecular level understanding of resistance to nalidixic acid in salmonella enteric serovar typhimurium associates with the s83f sequence type. | nalidixic acid is an antibiotic drug used for treatment of salmonellosis, a gastrointestinal infection. dna gyrase subunit a (gyra) of salmonella typhimurium is the drug target for nalidixic acid. resistance of gyra to nalidixic acid, because of a point mutation in s. typhimurium, was recently reported. substitution of phe in place of ser at locus 83 in gyra of s. typhimurium has been experimentally associated with nalidixic acid resistance. despite recent efforts, the mechanism of this resistan ... | 2016 | 26329667 |
| lipocalin-2 ensures host defense against salmonella typhimurium by controlling macrophage iron homeostasis and immune response. | lipocalin-2 (lcn2) is an innate immune peptide with pleiotropic effects. lcn2 binds iron-laden bacterial siderophores, chemo-attracts neutrophils and has immunomodulatory and apoptosis-regulating effects. in this study, we show that upon infection with salmonella enterica serovar typhimurium, lcn2 promotes iron export from salmonella-infected macrophages, which reduces cellular iron content and enhances the generation of pro-inflammatory cytokines. lcn2 represses il-10 production while augmentin ... | 2015 | 26332507 |
| residue-dependent thermodynamic cost and barrel plasticity balances activity in the phopq-activated enzyme pagp of salmonella typhimurium. | pagp is an eight-stranded transmembrane β-barrel enzyme indispensable for lipid a palmitoylation in gram-negative bacteria. the severity of infection by pathogens, including salmonella, legionella, and bordetella, and resistance to antimicrobial peptides, relies on lipid a remodeling by pagp, rendering pagp a sought-after drug target. despite a conserved sequence, more robust palmitoylation of lipid a is observed in salmonella typhimurium compared to escherichia coli, a possible consequence of t ... | 2015 | 26334694 |
| genotoxic, cytotoxic, antigenotoxic, and anticytotoxic effects of sulfonamide chalcone using the ames test and the mouse bone marrow micronucleus test. | chalcones present several biological activities and sulfonamide chalcone derivatives have shown important biological applications, including antitumor activity. in this study, genotoxic, cytotoxic, antigenotoxic, and anticytotoxic activities of the sulfonamide chalcone n-{4-[3-(4-nitrophenyl)prop-2-enoyl]phenyl} benzenesulfonamide (cpn) were assessed using the salmonella typhimurium reverse mutation test (ames test) and the mouse bone marrow micronucleus test. the results showed that cpn caused ... | 2015 | 26335560 |
| growth of healthy and sanitizer-injured salmonella cells on mung bean sprouts in different commercial enrichment broths. | the ability of nine commercial broths to enrich healthy and 90% sanitizer-injured salmonella typhimurium and salmonella cocktail on mung bean sprouts was evaluated to select an optimum broth for detection. results showed that s. typhimurium multiplied faster and reached a higher population in buffered peptone water (bpw), salmonella ad media (ad) and one broth-salmonella (ob), compared with other broths. healthy and 90% sanitizer-injured salmonella at low concentrations increased by 4.0 log cfu/ ... | 2015 | 26338131 |
| binding interface between the salmonella σ(s)/rpos subunit of rna polymerase and crl: hints from bacterial species lacking crl. | in many gram-negative bacteria, including salmonella enterica serovar typhimurium (s. typhimurium), the sigma factor rpos/σ(s) accumulates during stationary phase of growth, and associates with the core rna polymerase enzyme (e) to promote transcription initiation of genes involved in general stress resistance and starvation survival. whereas σ factors are usually inactivated upon interaction with anti-σ proteins, σ(s) binding to the crl protein increases σ(s) activity by favouring its associati ... | 2015 | 26338235 |
| deletion of invasion protein b in salmonella enterica serovar typhimurium influences bacterial invasion and virulence. | salmonella enterica serovar typhimurium (s. typhimurium) has a wide host range and causes infections ranging from severe gastroenteritis to systemic infections in human, as well as causing typhoid-like disease in murine models of infection. s. typhimurium translocates its effector proteins through the salmonella pathogenicity island-i (spi-i)-encoded t3ss-i needle complex. this study focuses on invasion protein b (sipb) of s. typhimurium, which plays an active role in spi-i invasion efficiency. ... | 2015 | 26341924 |
| graphene oxide-modified zno particles: synthesis, characterization, and antibacterial properties. | nanosized zno particles with diameters of 15 nm were prepared with a solution precipitation method at low cost and high yield. the synthesis of the particles was functionalized by the organic solvent dimethylformamide, and the particles were covalently bonded to the surface of graphene oxide. the morphology of the graphene oxide sheets and zno particles was confirmed with field emission scanning electron microscopy and biological atomic force microscopy. fourier transform infrared spectroscopy a ... | 2015 | 26347126 |
| neoadjuvant administration of semliki forest virus expressing interleukin-12 combined with attenuated salmonella eradicates breast cancer metastasis and achieves long-term survival in immunocompetent mice. | metastatic breast cancer is a major cause of death among women worldwide; therefore efficient therapeutic strategies are extremely needed. in this work we have developed a gene therapy- and bacteria-based combined neoadjuvant approach and evaluated its antitumor effect in a clinically relevant animal model of metastatic breast cancer. | 2015 | 26347489 |
| sugar-protein connectivity impacts on the immunogenicity of site-selective salmonella o-antigen glycoconjugate vaccines. | a series of glycoconjugates with defined connectivity were synthesized to investigate the impact of coupling salmonella typhimurium o-antigen to different amino acids of crm197 protein carrier. in particular, two novel methods for site-selective glycan conjugation were developed to obtain conjugates with single attachment site on the protein, based on chemical modification of a disulfide bond and ph-controlled transglutaminase-catalyzed modification of lysine, respectively. importantly, conjugat ... | 2015 | 26350581 |
| refined live attenuated salmonella enterica serovar typhimurium and enteritidis vaccines mediate homologous and heterologous serogroup protection in mice. | invasive nontyphoidal salmonella (nts) infections constitute a major health problem among infants and toddlers in sub-saharan africa; these infections also occur in infants and the elderly in developed countries. we genetically engineered a salmonella enterica serovar typhimurium strain of multilocus sequence type 313, the predominant genotype circulating in sub-saharan africa. we evaluated the capacities of s. typhimurium and salmonella enterica serovar enteritidis δguaba δclpx live oral vaccin ... | 2015 | 26351285 |
| chemical composition and in vitro antibacterial activity of mentha spicata essential oil against common food-borne pathogenic bacteria. | the aim of the present study was to investigate chemical composition and antibacterial activity of essential oil from the leaf of mentha spicata plant against common food-borne pathogenic bacteria (staphylococcus aureus, bacillus subtilis, bacillus cereus, listeria monocytogenes, salmonella typhimurium, and escherichia coli o157:h7). chemical composition of the essential oil was identified by gas chromatography coupled with mass spectrometer detector (gc-ms). the antibacterial activity of the es ... | 2015 | 26351584 |
| effect of detergents on galactoside binding by melibiose permeases. | the effect of various detergents on the stability and function of the melibiose permeases of escherichia coli (melbec) and salmonella typhimurium (melbst) was studied. in n-dodecyl-β-d-maltoside (ddm) or n-undecyl-β-d-maltoside (udm), wt melbst binds melibiose with an affinity similar to that in the membrane. however, with wt melbec or melbst mutants (arg141 → cys, arg295 → cys, or arg363 → cys), galactoside binding is not detected in these detergents, but binding to the phosphotransferase prote ... | 2015 | 26352464 |
| prevalence and characterization of monophasic salmonella serovar 1,4,[5],12:i:- of food origin in china. | salmonella enterica subsp. enterica serovar 1,4,[5],12:i:- is a monophasic variant of salmonella typhimurium, which has recently been recognized as an emerging cause of infection worldwide. this bacterium has also ranked among the four most frequent serovars causing human salmonellosis in china. however, there are no reports on its contamination in chinese food. serotyping, polymerase chain reaction, antibiotic resistance, virulotyping, and multilocus sequence typing (mlst) assays were used to i ... | 2015 | 26360603 |
| evaluation of the mutagenicity of simple substituted quinoxalines in salmonella typhimurium. | limited information is available on the genotoxicity of simple quinoxalines, distinct from the food related carcinogenic derivatives bearing an aromatic amino group. isolated positive results, with no apparent structure-activity relationships, were reported in earlier studies on alkyl substituted quinoxalines, raising a safety concern in some regulatory authorities in view of the potential human exposure related to their use as food flavors. in order to elucidate the genotoxic hazard posed by si ... | 2016 | 26365056 |
| photodecomposition, photomutagenicity and photocytotoxicity of retinyl palmitate under he-ne laser photoirradiation and its effects on photodynamic therapy of cancer cells in vitro. | our aim was to study photodecomposition, photomutagenicity and cytotoxicity of retinyl palmitate (rp), a principal storage form of vitamin a in humans and animals, under he-ne laser photoirradiation. moreover, the effect of different concentrations and timing protocol of antioxidants on photodynamic therapy (pdt) is contradictory, so the effect of rp (as antioxidant) on the pdt cytotoxicity was studied. | 2016 | 26365099 |
| multicopy single-stranded dna directs intestinal colonization of enteric pathogens. | multicopy single-stranded dnas (msdnas) are hybrid rna-dna molecules encoded on retroelements called retrons and produced by the action of retron reverse transcriptases. retrons are widespread in bacteria but the natural function of msdna has remained elusive despite 30 years of study. the major roadblock to elucidation of the function of these unique molecules has been the lack of any identifiable phenotypes for mutants unable to make msdna. we report that msdna of the zoonotic pathogen salmone ... | 2015 | 26367458 |
| next generation genome sequencing reveals phylogenetic clades with different level of virulence among salmonella typhimurium clinical human isolates in hong kong. | salmonella typhimurium is frequently isolated from foodborne infection cases in hong kong, but the lack of genome sequences has hindered in-depth epidemiological and phylogenetic studies. in this study, we sought to reconstruct the phylogenetic relationship and investigate the distribution and mutation patterns of virulence determinants among local s. typhimurium clinical isolates using their genome sequences. | 2015 | 26370680 |
| branched-chain amino acid supplementation promotes aerobic growth of salmonella typhimurium under nitrosative stress conditions. | nitric oxide (no) inactivates iron-sulfur enzymes in bacterial amino acid biosynthetic pathways, causing amino acid auxotrophy. we demonstrate that exogenous supplementation with branched-chain amino acids (bcaa) can restore the no resistance of hmp mutant salmonella typhimurium lacking principal no-metabolizing enzyme flavohemoglobin, and of mutants further lacking iron-sulfur enzymes dihydroxy-acid dehydratase (ilvd) and isopropylmalate isomerase (leucd) that are essential for bcaa biosynthesi ... | 2015 | 26374245 |
| therapeutic efficacy of tumor-targeting salmonella typhimurium a1-r on human colorectal cancer liver metastasis in orthotopic nude-mouse models. | liver metastasis is the most frequent cause of death from colon and other cancers. generally, liver metastasis is recalcitrant to treatment. the aim of this study is to determine the efficacy of tumor-targeting salmonella typhimurium a1-r on liver metastasis in orthotopic mouse models. ht-29 human colon cancer cells expressing red fluorescent protein (rfp) were used in the present study. s. typhimurium a1-r infected ht-29 cells in a time-dependent manner, inhibiting cancer-cell proliferation in ... | 2015 | 26375054 |
| caspase-11 cleaves gasdermin d for non-canonical inflammasome signalling. | intracellular lipopolysaccharide from gram-negative bacteria including escherichia coli, salmonella typhimurium, shigella flexneri, and burkholderia thailandensis activates mouse caspase-11, causing pyroptotic cell death, interleukin-1β processing, and lethal septic shock. how caspase-11 executes these downstream signalling events is largely unknown. here we show that gasdermin d is essential for caspase-11-dependent pyroptosis and interleukin-1β maturation. a forward genetic screen with ethyl-n ... | 2015 | 26375259 |
| an orally administered dna vaccine targeting vascular endothelial growth factor receptor-3 inhibits lung carcinoma growth. | lung cancer is the leading cause of mortality and 5-year survival rate is very low worldwide. recent studies show that vascular endothelial growth factor receptor-3 (vegfr-3) signaling pathway contributes to lung cancer progression. so we hypothesize that an oral dna vaccine that targets vegfr-3 carried by attenuated salmonella enterica serovar typhimurium strain sl3261 has impacts on lung cancer progression. in this study, the oral vegfr-3-based vaccine-immunized mice showed appreciable inhibit ... | 2016 | 26376999 |
| antibacterial activities of ankaferd hemostat (abs) on shiga toxin-producing escherichia coli and other pathogens significant in foodborne diseases. | ankaferd hemostat (ankaferd blood stopper®, abs)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. topical endoscopic abs application is effective in the controlling of gastrointestinal (gi) system hemorrhages and/or infected gi wounds. escherichia coli o157:h7, the predominant serotype of enterohemorrhagic e. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. the aim of this study i ... | 2017 | 26377624 |
| development and evaluation of live attenuated salmonella vaccines in newly hatched duckings. | domestic ducks remain a major source of zoonotic salmonella enterica infections for man worldwide and approaches to protection should include vaccine-mediated immunity. with this in mind we developed several genetically defined mutants in a virulent duck salmonella typhimurium isolate tt-1. from initial tests for virulence in day-old ducks, δrpos, δhila, and δslya mutants retained some virulence so were not studied further. amongst the mutants showing greater attenuation, δssrb, δphopq, δompr, a ... | 2015 | 26382600 |
| evaluation of safety of arrabidaea chica verlot (bignoniaceae), a plant with healing properties. | arrabidaea chica verlot (bignoniaceae) has been used as a medicinal herb to treat anemia, hemorrhage, inflammation, intestinal colic, hepatitis, and skin infections in the brazilian amazon region. studies have demonstrated the healing properties of extracts obtained from a. chica leaves, which contain anthocyanins and flavonoids. however, few investigations have assessed the safe use of this plant species. in this study, mutagenic and genotoxic effects of a crude aqueous extract, a butanolic fra ... | 2015 | 26383782 |
| emergence of vim-1-producing salmonella enterica serovar typhimurium in a paediatric patient. | 2015 | 26384861 | |
| using uvc light-emitting diodes at wavelengths of 266 to 279 nanometers to inactivate foodborne pathogens and pasteurize sliced cheese. | uvc light is a widely used sterilization technology. however, uv lamps have several limitations, including low activity at refrigeration temperatures, a long warm-up time, and risk of mercury exposure. uv-type lamps only emit light at 254 nm, so as an alternative, uv light-emitting diodes (uv-leds) which can produce the desired wavelengths have been developed. in this study, we validated the inactivation efficacy of uv-leds by wavelength and compared the results to those of conventional uv lamps ... | 2015 | 26386061 |
| global transcriptome and mutagenic analyses of the acid tolerance response of salmonella enterica serovar typhimurium. | salmonella enterica serovar typhimurium (s. typhimurium) is one of the leading causative agents of food-borne bacterial gastroenteritis. swift invasion through the intestinal tract and successful establishment in systemic organs are associated with the adaptability of s. typhimurium to different stress environments. low-ph stress serves as one of the first lines of defense in mammalian hosts, which s. typhimurium must efficiently overcome to establish an infection. therefore, a better understand ... | 2015 | 26386064 |
| blu-ray optomagnetic measurement based competitive immunoassay for salmonella detection. | a turn-on competitive immunoassay using a low-cost blu-ray optomagnetic setup and two differently sized magnetic particles (micron-sized particles acting as capture particles and nano-sized particles acting as detection particles) is here presented. for salmonella detection, a limit of detection of 8×10(4)cfu/ml is achieved within a total assay time of 3h. the combination of a competitive strategy and an optomagnetic setup not only enables a turn-on read-out format, but also results in a sensiti ... | 2016 | 26386328 |
| quantitative proteomics and bioinformatic analysis provide new insight into the dynamic response of porcine intestine to salmonella typhimurium. | the enteropathogen salmonella typhimurium (s. typhimurium) is the most commonly non-typhoideal serotype isolated in pig worldwide. currently, one of the main sources of human infection is by consumption of pork meat. therefore, prevention and control of salmonellosis in pigs is crucial for minimizing risks to public health. the aim of the present study was to use isobaric tags for relative and absolute quantification (itraq) to explore differences in the response to salmonella in two segment of ... | 2015 | 26389078 |
| chip-seq analysis of the σe regulon of salmonella enterica serovar typhimurium reveals new genes implicated in heat shock and oxidative stress response. | the alternative sigma factor σe functions to maintain bacterial homeostasis and membrane integrity in response to extracytoplasmic stress by regulating thousands of genes both directly and indirectly. the transcriptional regulatory network governed by σe in salmonella and e. coli has been examined using microarray, however a genome-wide analysis of σe-binding sites in salmonella has not yet been reported. we infected macrophages with salmonella typhimurium over a select time course. using chroma ... | 2015 | 26389830 |
| trim38 negatively regulates tlr3/4-mediated innate immune and inflammatory responses by two sequential and distinct mechanisms. | tripartite motif (trim)38 is an e3 ubiquitin ligase that was reported to regulate signaling in innate immune and inflammatory responses in certain cell lines. in this study, we show that trim38 deficiency markedly increased tlr3- and tlr4-mediated induction of type i ifns and proinflammatory cytokines, such as tnf-α, il-1β, and il-6, in immune cells and in vivo. trim38 deficiency also caused the mice to be more susceptible to death triggered by polyinosinic-polycytidylic acid, lps, and salmonell ... | 2015 | 26392463 |
| ssek3 is a salmonella effector that binds trim32 and modulates the host's nf-κb signalling activity. | salmonella typhimurium employs an array of type iii secretion system effectors that facilitate intracellular survival and replication during infection. the salmonella effector ssek3 was originally identified due to amino acid sequence similarity with nleb; an effector secreted by epec/ehec that possesses n-acetylglucoasmine (glcnac) transferase activity and modifies death domain containing proteins to block extrinsic apoptosis. in this study, immunoprecipitation of ssek3 defined a novel molecula ... | 2015 | 26394407 |
| modulation of cytokine gene expression by selected lactobacillus isolates in the ileum, caecal tonsils and spleen of salmonella-challenged broilers. | probiotics have been used to control salmonella colonization in the chicken intestine. recently, we demonstrated that certain selected lactobacillus isolates were able to reduce salmonella infection in the chicken spleen and liver as well as down-regulated salmonella pathogenicity island 1 virulence gene expression in the chicken caecum. to further understand the mechanisms through which lactobacillus protected chickens from salmonella infection, the present study has investigated the lactobacil ... | 2015 | 26395945 |
| rosa canina l.--new possibilities for an old medicinal herb. | health beneficial properties of rosa canina species are mainly attributed to rose hips, while the leaves are usually discarded as waste. in the present study we investigated chemical constituents as well as antimicrobial and antibiofilm potential of r. canina methanolic leaf extract. chemical analysis showed that dominant phenolic compounds are quercetin and isorhamnetin derivatives (isoquercetin and isorhamnetin-3-o-rutinoside). among the tested bacteria, pseudomonas aeruginosa and salmonella t ... | 2015 | 26399901 |
| secondary metabolites constituents and antioxidant, anticancer and antibacterial activities of etlingera elatior (jack) r.m.sm grown in different locations of malaysia. | etlingera elatior is a well-known herb in malaysia with various pharmaceutical properties. | 2015 | 26399961 |
| [disinfectants - bacterial cells interactions in the view of hygiene and public health]. | in recent years, the use of biocides has increased rapidly. one common example is triclosan, with wide application in households as well as medical and industrial fields, especially food industry and animal husbandry. chemical disinfection is a major mean to control and eliminate pathogenic bacteria, particularly those with multidrug resistance (mdr) phenotype. however, exposition to biocides results in an adaptive response in microorganisms, causing them to display a wide range of resistance me ... | 2015 | 26400890 |
| perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. | perforin-2 (mpeg1) is a pore-forming, antibacterial protein with broad-spectrum activity. perforin-2 is expressed constitutively in phagocytes and inducibly in parenchymal, tissue-forming cells. in vitro, perforin-2 prevents the intracellular replication and proliferation of bacterial pathogens in these cells. perforin-2 knockout mice are unable to control the systemic dissemination of methicillin-resistant staphylococcus aureus (mrsa) or salmonella typhimurium and perish shortly after epicutane ... | 2015 | 26402460 |
| isolation and characterization of bacteriocinogenic lactic bacteria from m-tuba and tepache, two traditional fermented beverages in méxico. | mexican tuba (m-tuba) and tepache are mexican fermented beverages prepared mainly with pineapple pulp and coconut palm, respectively. at present, reports on the microbiota and nutritional effects of both beverages are lacking. the purpose of this study was to determine whether m-tuba and tepache contain cultivable lactic acid bacteria (lab) capable of producing bacteriocins. tepache and m-tuba contain mesophilic aerobic bacteria, lab, and yeast. bacillus subtilis, listeria monocytogenes, listeri ... | 2015 | 26405529 |
| rapid detection of salmonella enterica serovar typhimurium dt104 strains by the polymerase chain reaction. | infection with salmonella enterica is a major public health concern in developed countries, and multidrug-resistant strains have become increasingly prevalent. s. enterica serovar typhimurium dt104 (dt104) strains are prevalent in livestock in japan and include numerous strains of multidrug-resistant s. enterica. epidemiological analysis of these strains is critical for both agriculture and public health; however, diagnostic tests for these strains have yielded inconsistent results. | 2015 | 26408088 |