Publications

rapid high-yield expression of a candidate influenza vaccine based on the ectodomain of m2 protein linked to flagellin in plants using viral vectors.	the extracellular domain of matrix protein 2 (m2e) of influenza a virus is a promising target for the development of a universal vaccine against influenza because m2e sequences are highly conserved among human influenza a strains. however, native m2e is poorly immunogenic, but its immunogenicity can be increased by delivery in combination with adjuvants or carrier particles. it was previously shown that fusion of m2e to bacterial flagellin, the ligand for toll-like receptor (tlr) 5 and powerful ...	2015	26022390
quantitative evaluation of dna damage and mutation rate by atmospheric and room-temperature plasma (artp) and conventional mutagenesis.	dna damage is the dominant source of mutation, which is the driving force of evolution. therefore, it is important to quantitatively analyze the dna damage caused by different mutagenesis methods, the subsequent mutation rates, and their relationship. atmospheric and room temperature plasma (artp) mutagenesis has been used for the mutation breeding of more than 40 microorganisms. however, artp mutagenesis has not been quantitatively compared with conventional mutation methods. in this study, the ...	2015	26025015
microbiological survey of birds of prey pellets.	a microbiological survey of 73 pellets collected from different birds of prey species housed at the wildlife rescue and rehabilitation center of napoli (southern italy) was performed. pellets were analyzed by culture and biochemical methods as well as by serotyping and polymerase chain reaction. we isolated a wide range of bacteria some of them also pathogens for humans (i.e. salmonella enterica serotype typhimurium, campylobacter coli, escherichia coli o serogroups). this study highlights the p ...	2015	26026881
antimutagenic and anticancer activity of al madinah alhasawy mint (mentha longifolia) leaves extract.	mentha is one of the genera of lamiaceae family. the aim of the present study was to evaluate the antimutagenic and anticancer activity of the water and methanolic extract of alhasawy mint (mentha longifolia), that grown in madina province, western region, saudi arabia using three different bioassays namely; brine shrimp bioassay, ames mutagenicity bioassay using 3 hist-salmonella typhimurium strains of different mutations (ta98, ta97 and ta100) and 2 reference mutagenic drugs nitrosopiperidine ...	2014	26027170
interferon-gamma and nitric oxide synthase 2 mediate the aggregation of resident adherent peritoneal exudate cells: implications for the host response to pathogens.	interferon-gamma (ifnγ), a key macrophage activating cytokine, plays pleiotropic roles in host immunity. in this study, the ability of ifnγ to induce the aggregation of resident mouse adherent peritoneal exudate cells (apecs), consisting primarily of macrophages, was investigated. cell-cell interactions involve adhesion molecules and, upon addition of ifnγ, cd11b re-localizes preferentially to the sites of interaction on apecs. a functional role of cd11b in enhancing aggregation is demonstrated ...	2015	26029930
burkholderia cenocepacia and salmonella enterica arnt proteins that transfer 4-amino-4-deoxy-l-arabinose to lipopolysaccharide share membrane topology and functional amino acids.	we recently demonstrated that incorporation of 4-amino-4-deoxy-l-arabinose (l-ara4n) to the lipid a moiety of lipopolysaccharide (lps) is required for transport of lps to the outer membrane and viability of the gram-negative bacterium burkholderia cenocepacia. arnt is a membrane protein catalyzing the transfer of l-ara4n to the lps molecule at the periplasmic face of the inner membrane, but its topology and mechanism of action are not well characterized. here, we elucidate the topology of arnt a ...	2015	26030265
outbreak of uncommon o4 non-agglutinating salmonella typhimurium linked to minced pork, saxony-anhalt, germany, january to april 2013.	in january 2013, the national reference centre for salmonella (nrc) detected a salmonellosis cluster in saxony-anhalt, germany, caused by uncommon o4 non-agglutinating, monophasic salmonella (s.) typhimurium dt193. circulating predominant monophasic s. typhimurium dt193 clones typically display resistance phenotype assut. we investigated common exposures to control the outbreak, and conducted microbiological investigations to assess the strains' phenotype.	2015	26030424
a mutagenicity and cytotoxicity study of limonium effusum aqueous extracts by allium, ames and mtt tests.	nowadays plants or plant extracts have become very important for alternative medicine. plants and their extracts have many therapeutical advantages but some of them are potentially toxic, mutagenic, carcinogenic and teratogenic. root, stem and leafparts of limonium effusum were used in this study and this species is an endemic species for turkey. mutagenic and cytotoxic effects of root, stem and leaf aqueous extracts were observed with allium, ames and mtt tests. allium root growth inhibition te ...	2015	26030975
antimicrobial and cytotoxic activities from jatropha dioica roots.	the antimicrobial and cytotoxic activities of organic extracts obtained from roots of the medicinal plant jatropha dioica (euphorbiaceae) were investigated. in order to evaluate their antimicrobial activity, the organic extracts were tested against clinical isolates of the human pathogens bacillus cereus, escherichia coli, salmonella typhi, staphylococcus aureus, enterobacter aerogenes, enterobacter cloacae, salmonella typhimurium, cryptococcus neoformans, candida albicans, candida parapsilosis ...	2014	26031013
synergistic effect of slightly acidic electrolyzed water and ultrasound at mild heat temperature in microbial reduction and shelf-life extension of fresh-cut bell pepper.	the objectives of this study were to evaluate the effect of combined treatments (slightly acidic electrolyzed water (saew), ultrasound (us), or mild heat (60°c)) on the growth of listeria monocytogenes and salmonella enterica serovar typhimurium in fresh-cut bell pepper, and the shelf-life and sensory quality (color and texture) were followed during storage at 4°c and 25°c. an additional 0.65, 1.72, and 2.70 log cfu/g reduction was achieved by heat treatments at 60°c for 1 min for dw, saew, and ...	2015	26032362
inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in apple juice at different ph levels by gaseous ozone treatment.	we investigated the effect of ozone treatment of apple juice at different ph levels for inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes.	2015	26033289
antimicrobial activities of the combined use of cuminum cyminum l. essential oil, nisin and storage temperature against salmonella typhimurium and staphylococcus aureus in vitro.	foodborne diseases are considered as major health problems in different countries. concerns over the safety of some chemical preservatives and negative consumer reactions to them have prompted interest in natural alternatives for the maintenance or extension of food shelf life. in this respect, the combination of a plant essential oil and nisin has used for controlling the growth of foodborne pathogens as natural food preservative using the mathematical model.	2015	26034554
kinetics of bacterial inactivation by 405nm and 520nm light emitting diodes and the role of endogenous coproporphyrin on bacterial susceptibility.	photodynamic inactivation studies of microbial pathogens have focused on the use of an external photosensitizer or a precursor compound to eliminate bacteria. the present study investigated the inactivation kinetics of six bacterial pathogens by a 405nm light emitting diode (led) without the addition of any external compound. the role of endogenous coproporphyrin on the bacterial susceptibility to leds was also examined. pathogens were illuminated with leds at 25, 10 and 4°c for 9h and the inact ...	2015	26036659
soluble flagellin coimmunization attenuates th1 priming to salmonella and clearance by modulating dendritic cell activation and cytokine production.	soluble flagellin (sflic) from salmonella typhimurium (stm) can induce a th2 response to itself and coadministered antigens through ligation of tlr5. these properties suggest that sflic could potentially modulate responses to th1 antigens like live stm if both antigens are given concurrently. after coimmunization of mice with sflic and stm there was a reduction in th1 t cells (t-bet(+) ifn-γ(+) cd4 t cells) compared to stm alone and there was impaired clearance of stm. in contrast, there was no ...	2015	26036767
comparative effects of ohmic and conventional heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in skim milk and cream.	ohmic heating has proven advantages over conventional thermal processing and novel thermal alternative technologies. in this study, the effect of ohmic and conventional heating for pasteurizing skim milk and cream was examined. all treatment conditions for ohmic and conventional heating were identical except for composition of the heating chamber. in most cases, the reduction of three pathogens did not differ significantly between ohmic heating and conventional heating at fixed treatment tempera ...	2015	26038915
antimicrobial effects of nisin, essential oil, and γ-irradiation treatments against high load of salmonella typhimurium on mini-carrots.	this study aimed at using essential oil (eo) alone or combined eo with nisin and γ-irradiation to control salmonella typhimurium during the refrigerated storage of mini-carrots. peeled mini-carrots were inoculated with s. typhimurium at a final concentration of approximately 7 log cfu/g. inoculated samples were coated by 5 different coating solutions: (i) nisin solution at final concentration of 10(3) iu/ml; (ii) mountain savory eo solution at 0.35%; (iii) carvacrol solution at 0.35%; (iv) mount ...	2015	26040299
effect of milk fat content on the performance of ohmic heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes.	the effect of milk fat content on ohmic heating compared to conventional heating for inactivation of food-borne pathogens was investigated.	2015	26043029
antibacterial activity of ovary extract from sea urchin diadema setosum.	sea urchin gonad is considered as a highly prized delicacy in several countries. it is also rich in valuable bioactive compounds including polyunsaturated fatty acids (pufas) and β-carotene. this study was undertaken to examine the antimicrobial properties of the ovary extract from sea urchin diadema setosum against selected gram-negative and gram-positive bacteria.	2015	26044237
low dietary iron intake restrains the intestinal inflammatory response and pathology of enteric infection by food-borne bacterial pathogens.	orally administrated iron is suspected to increase susceptibility to enteric infections among children in infection endemic regions. here we investigated the effect of dietary iron on the pathology and local immune responses in intestinal infection models. mice were held on iron-deficient, normal iron, or high iron diets and after 2 weeks they were orally challenged with the pathogen citrobacter rodentium. microbiome analysis by pyrosequencing revealed profound iron- and infection-induced shifts ...	2015	26046550
tumor-targeting salmonella typhimurium a1-r in combination with trastuzumab eradicates her-2-positive cervical cancer cells in patient-derived mouse models.	we have previously developed mouse models of her-2-positive cervical cancer. tumors in nude mice had histological structures similar to the original tumor and were stained by anti-her-2 antibody in the same pattern as the patient's cancer. we have also previously developed tumor-targeting salmonella typhimurium a1-r and have demonstrated its efficacy against patient-derived tumor mouse models, both alone and in combination. in the current study, we determined the efficacy of s. typhimurium a1-r ...	2015	26047477
flagellin is required for host cell invasion and normal salmonella pathogenicity island 1 expression by salmonella enterica serovar paratyphi a.	salmonella enterica serovar paratyphi a is a human-specific serovar that, together with salmonella enterica serovar typhi and salmonella enterica serovar sendai, causes enteric fever. unlike the nontyphoidal salmonella enterica serovar typhimurium, the genomes of s. typhi and s. paratyphi a are characterized by inactivation of multiple genes, including in the flagellum-chemotaxis pathway. here, we explored the motility phenotype of s. paratyphi a and the role of flagellin in key virulence-associ ...	2015	26056383
effect of cell immobilization on the growth dynamics of salmonella typhimurium and escherichia coli at suboptimal temperatures.	predictive microbiology has recently acknowledged the impact of the solid(like) food structure on microbial behavior. the presence of this solid(like) structure causes microorganisms to grow as colonies and no longer planktonically as in liquid. in this paper, the growth dynamics of salmonella typhimurium and escherichia coli were studied as a function of temperature, considering different growth morphologies, i.e., (i) planktonic cells, (ii) immersed colonies and (iii) surface colonies. for all ...	2015	26057111
small molecules with big effects: cyclic di-gmp-mediated stimulation of cellulose production by the amino acid ʟ-arginine.	in this issue of science signaling, mills et al. show that the amino acid ʟ-arginine increases the concentration of the second messenger c-di-gmp in salmonella enterica serovar typhimurium through a specific diguanylate cyclase, leading to increased production of the exopolysaccharide cellulose, which is an extracellular matrix component of environmental and host-associated biofilms.	2015	26060328
a direct screen for c-di-gmp modulators reveals a salmonella typhimurium periplasmic ʟ-arginine-sensing pathway.	cyclic-di-gmp (c-di-gmp) is a bacterial second messenger that transduces internal and external signals and regulates bacterial motility and biofilm formation. some organisms encode more than 100 c-di-gmp-modulating enzymes, but only for a few has a signal been defined that modulates their activity. we developed and applied a high-throughput, real-time flow cytometry method that uses a fluorescence resonance energy transfer (fret)-based biosensor of free c-di-gmp to screen for signals that modula ...	2015	26060330
diffusion and persistence of multidrug resistant salmonella typhimurium strains phage type dt120 in southern italy.	sixty-two multidrug resistant salmonella enterica serovar typhimurium strains isolated from 255 clinical strains collected in southern italy in 2006-2008 were characterised for antimicrobial resistance genes, pulsotype, and phage type. most strains (83.9%) were resistant to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline (acssut) encoded in 88.5% by the salmonella genomic island (sgi1) and in 11.5% by the inh-like integron (bla oxa-30-aada1) and cata1, sul1, and tet ...	2015	26060815
molecular epidemiology of nontyphoidal salmonella in poultry and poultry products in india: implications for human health.	human infections with non-typhoidal salmonella (nts) serovars are increasingly becoming a threat to human health globally. while all motile salmonellae have zoonotic potential, salmonella enteritidis and salmonella typhimurium are most commonly associated with human disease, for which poultry are a major source. despite the increasing number of human nts infections, the epidemiology of nts in poultry in india has not been fully understood. hence, as a first step, we carried out epidemiological a ...	2015	26063942
herd- and individual-level prevalences of and risk factors for salmonella spp. fecal shedding in dairy farms in al-dhulail valley, jordan.	salmonellosis is an important disease frequently associated with diarrhea in calves. from january to september 2009, a cross-sectional study involving 91 dairy farms was conducted to determine the prevalence of salmonella spp. infection in cattle in al-dhulail valley, jordan. a total of 910 calve and cow fecal samples were collected. information on farm management practices was obtained through personal interviews using a standardized questionnaire and was tested as risk factors for salmonella s ...	2015	26065698
bacteria moving into focus of human cancer.	although bacteria have long been associated with human cancer, drawing causal relationships has been difficult. in this issue of cell host & microbe, scanu et al. (2015) provide evidence for a transforming activity of salmonella typhimurium on predisposed host cells, which can subsequently form tumors in a xenograft model.	2015	26067598
in vitro and in vivo toxicological evaluations of methyl ferulate, methyl p-coumarate, and pulegone 1,2-epoxide.	toxicological screening of natural compounds for medicinal purposes.	2016	26067677
infection with salmonella enterica serovar typhimurium leads to increased proportions of f4/80+ red pulp macrophages and decreased proportions of b and t lymphocytes in the spleen.	infection of mice with salmonella enterica serovar typhimurium (salmonella) causes systemic inflammatory disease and enlargement of the spleen (splenomegaly). splenomegaly has been attributed to a general increase in the numbers of phagocytes, lymphocytes, as well as to the expansion of immature cd71+ter119+ reticulocytes. the spleen is important for recycling senescent red blood cells (rbcs) and for the capture and eradication of blood-borne pathogens. conservation of splenic tissue architectur ...	2015	26068006
statistical approaches to developing a multiplex immunoassay for determining human exposure to environmental pathogens.	there are numerous pathogens that can be transmitted through water. identifying and understanding the routes and magnitude of exposure or infection to these microbial contaminants are critical to assessing and mitigating risk. conventional approaches of studying immunological responses to exposure or infection such as enzyme-linked immunosorbent assays (elisas) and other monoplex antibody-based immunoassays can be very costly, laborious, and consume large quantities of patient sample. a major li ...	2015	26070441
acetylation regulates survival of salmonella enterica serovar typhimurium under acid stress.	the ability to acetylate lysine residues is conserved across organisms, and acetylation of lysine residues plays important roles in various cellular functions. maintaining intracellular ph homeostasis is crucial for the survival of enteric bacteria in the acidic gastric tract. it has been shown that eukaryotes can stabilize the intracellular ph by histone deacetylation. however, it remains unknown whether bacteria can utilize a reversible protein acetylation system to adapt to an acidic environm ...	2015	26070677
an enterobacterial common antigen mutant of salmonella enterica serovar typhimurium as a vaccine candidate.	due to increasing rates of invasive salmonella enterica serovar typhimurium infection, there is a need for an effective vaccine to prevent this disease. previous studies showed that a mutation in the first gene of the enterobacterial common antigen biosynthetic pathway, weca, resulted in attenuation of s. typhimurium in a murine model of salmonellosis. furthermore, immunization with a weca(-) strain protected against lethal challenge with the parental wild type s. typhimurium strain. herein, we ...	2015	26070977
back to the future: are tumor-targeting bacteria the next-generation cancer therapy?	cancer patients infected with various bacteria were reported, for at least two centuries, to have spontaneous remission. w.b. coley, of what is now the memorial sloan-kettering cancer center, pioneered bacterial therapy of cancer in the clinic with considerable success beginning in the late nineteenth century. after coley died in 1936, bacterial therapy of cancer essentially ended. currently there is much excitement in developing bacterial therapy for treating cancer using either obligate or fac ...	2015	26072411
synergistic antibacterial effect of silver nanoparticles combined with ineffective antibiotics on drug resistant salmonella typhimurium dt104.	synergistic antibacterial activity of combined silver nanoparticles (agnps) with tetracycline (polykeptide), neomycin (aminoglycoside), and penicillin (β-lactam) was tested against the multidrug resistant bacterium salmonella typhimurium dt104. dose-dependent inhibition of salmonella typhimurium dt104 growth is observed for tetracycline-agnps and neomycin-agnps combination with ic50 of 0.07 μg/ml and 0.43 μg/ml, respectively. there is no inhibition by the penicillin-agnps combination. these resu ...	2015	26072671
toxicogenetic effects of low concentrations of the pesticides imidacloprid and sulfentrazone individually and in combination in in vitro tests with hepg2 cells and salmonella typhimurium.	the insecticide imidacloprid and the herbicide sulfentrazone are two different classes of pesticides that are used for pest control in sugarcane agriculture. to evaluate the genotoxic potential of low concentrations of these two pesticides alone and in mixture, the comet assay and the micronucleus (mn) test employing fluorescence in situ hybridization (fish) with a centromeric probe were applied in human hepatoma cell lines (hepg2), in a 24-h assay. mutagenicity was assessed by salmonella/micros ...	2015	26074310
characterization of salmonella enterica serovar typhimurium and salmonella enterica serovar 4,[5],12:i:- isolates from pigs presenting with diarrhea in korea.	between 2011 and 2012, a total of 896 pig fecal samples were collected from nine provinces in korea, and 50 salmonella enterica susp. enterica serovar typhimurium (s. typhimurium) was isolated. the characteristics of the 50 strains were analyzed, and 4 strains were identified as salmonella enterica subsp. enterica serovar 4,[5],12:i:-. salmonella 4,[5],12:i:- could not be distinguished from s. typhimurium through phage typing, antimicrobial resistance testing or multiple-locus variable-number ta ...	2015	26074410
melissa officinalis l. decoctions as functional beverages: a bioactive approach and chemical characterization.	lemon balm (melissa officinalis l.) is a member of the lamiaceae family with a long story of human consumption. it has been consumed for decades, directly in food and as a decoction or an infusion for its medicinal purposes. in this manuscript, a detailed chemical characterization of the decoction of this plant is described, encompassing antimicrobial, antioxidant and antitumor activities. rosmarinic acid and lithospermic acid a were the most abundant phenolic compounds. quinic acid, fructose, g ...	2015	26075899
correction: signatures of adaptation in human invasive salmonella typhimurium st313 populations from sub-saharan africa.		2015	26076129
fluoroquinolone induction of phage-mediated gene transfer in multidrug-resistant salmonella.	fluoroquinolones are broad-spectrum antibiotics that inhibit bacterial dna gyrase and topoisomerase activity, which can cause dna damage and result in bacterial cell death. in response to dna damage, bacteria induce an sos response to stimulate dna repair. however, the sos response may also induce prophage with production of infectious virions. salmonella strains typically contain multiple prophages, and certain strains including phage types dt120 and dt104 contain prophage that upon induction a ...	2015	26078016
crystal structure of dsba from corynebacterium diphtheriae and its functional implications for cuep in gram-positive bacteria.	in gram-negative bacteria in the periplasmic space, the dimeric thioredoxin-fold protein dsbc isomerizes and reduces incorrect disulfide bonds of unfolded proteins, while the monomeric thioredoxin-fold protein dsba introduces disulfide bonds in folding proteins. in the gram-negative bacteria salmonella enterica serovar typhimurium, the reduced form of cuep scavenges the production of hydroxyl radicals in the copper-mediated fenton reaction, and dsbc is responsible for keeping cuep in the reduced ...	2015	26082031
the anti-infective activity of punicalagin against salmonella enterica subsp. enterica serovar typhimurium in mice.	punicalagin, a major bioactive component of pomegranate peel, has been proven to have antioxidant, antiviral, anti-apoptosis, and hepatoprotective properties. the aim of this study was to investigate the anti-infective activity of punicalagin in a mouse model. c57bl/6 mice were initially challenged with salmonella enterica subsp. enterica serovar typhimurium (s. typhimurium) and then treated with punicalagin. food and water consumption and body weight were recorded daily. on day 8 post infection ...	2015	26084785
pichia pastoris x-33 has probiotic properties with remarkable antibacterial activity against salmonella typhimurium.	probiotics are live microorganisms which are beneficial for the host when ingested at high enough concentrations. the methylotrophic yeast pichia pastoris is widely used as heterologous protein production platform. however, its use as probiotic is poorly studied. the objective of this study was to evaluate some probiotic properties of the p. pastoris strain x-33 wild type. the resistance to in vitro and in vivo gastrointestinal conditions, stability in feed, safety, and antibacterial activity ag ...	2015	26088173
fatty acids composition and biofilm production of attenuated salmonella typhimurium dam and seqa mutants after exposure to uv-c.	the goal of this work was the investigation of correlation between some peculiarities of membrane fatty acids composition, biofilm formation, and motility of dam and/or seqa mutants in salmonella typhimurium bacterial cells and uv-c radiations. the exposure changed the fatty acids composition of dam and seqa/dam strains. significant increase of unsaturated fatty acids was observed. swarming and swimming were enhanced only in dam mutant and biofilm formation increased significantly in all tested ...	2015	26088950
complete genome sequencing of a multidrug-resistant and human-invasive salmonella enterica serovar typhimurium strain of the emerging sequence type 213 genotype.	salmonella enterica subsp. enterica serovar typhimurium strain yu39 was isolated in 2005 in the state of yucatán, mexico, from a human systemic infection. the yu39 strain is representative of the multidrug-resistant emergent sequence type 213 (st213) genotype. the yu39 complete genome is composed of a chromosome and seven plasmids.	2015	26089426
characterization of the invasive, multidrug resistant non-typhoidal salmonella strain d23580 in a murine model of infection.	a distinct pathovar of salmonella enterica serovar typhimurium, st313, has emerged in sub-saharan africa as a major cause of fatal bacteremia in young children and hiv-infected adults. d23580, a multidrug resistant clinical isolate of st313, was previously shown to have undergone genome reduction in a manner that resembles that of the more human-restricted pathogen, salmonella enterica serovar typhi. it has since been shown through tissue distribution studies that d23580 is able to establish an ...	2015	26091096
cold plasma inactivation of internalised bacteria and biofilms for salmonella enterica serovar typhimurium, listeria monocytogenes and escherichia coli.	microbial biofilms and bacteria internalised in produce tissue may reduce the effectiveness of decontamination methods. in this study, the inactivation efficacy of in-package atmospheric cold plasma (acp) afterglow was investigated against salmonella typhimurium, listeria monocytogenes and escherichia coli in the forms of planktonic cultures, biofilms formed on lettuce and associated bacteria internalised in lettuce tissue. prepared lettuce broth (3%) was inoculated with bacteria resulting in a ...	2015	26093991
salmonella typhimurium and escherichia coli dissimilarity: closely related bacteria with distinct metabolic profiles.	live attenuated strains of salmonella typhimurium have been extensively investigated as vaccines for a number of infectious diseases. however, there is still little information available concerning aspects of their metabolism. s. typhimurium and escherichia coli show a high degree of similarity in terms of their genome contents and metabolic networks. however, this work presents experimental evidence showing that significant differences exist in their abilities to direct carbon fluxes to biomass ...	2015	26097206
inactivation of pathogenic bacteria inoculated onto a bacto™ agar model surface using tio2-uvc photocatalysis, uvc and chlorine treatments.	the aim of this study was to study inactivation of different pathogenic bacteria on agar model surface using tio2-uv photocatalysis (tuvp).	2015	26099167
the periplasmic nitrate reductase napabc supports luminal growth of salmonella enterica serovar typhimurium during colitis.	the food-borne pathogen salmonella enterica serovar typhimurium benefits from acute inflammation in part by using host-derived nitrate to respire anaerobically and compete successfully with the commensal microbes during growth in the intestinal lumen. the s. typhimurium genome contains three nitrate reductases, encoded by the narghi, narzyv, and napabc genes. work on homologous genes present in escherichia coli suggests that nitrate reductase a, encoded by the narghi genes, is the main enzyme pr ...	2015	26099579
salmonella engages host micrornas to modulate sumoylation: a new arsenal for intracellular survival.	posttranslational modifications (ptms) can alter many fundamental properties of a protein. one or combinations of them have been known to regulate the dynamics of many cellular pathways and consequently regulate all vital processes. understandably, pathogens have evolved sophisticated strategies to subvert these mechanisms to achieve instantaneous control over host functions. here, we present the first report of modulation by intestinal pathogen salmonella enterica serovar typhimurium (s. typhim ...	2015	26100020
a mannose family phosphotransferase system permease and associated enzymes are required for utilization of fructoselysine and glucoselysine in salmonella enterica serovar typhimurium.	salmonella enteric serovar typhimurium, a major cause of food-borne illness, is capable of using a variety of carbon and nitrogen sources. fructoselysine and glucoselysine are maillard reaction products formed by the reaction of glucose or fructose, respectively, with the ε-amine group of lysine. we report here that s. typhimurium utilizes fructoselysine and glucoselysine as carbon and nitrogen sources via a mannose family phosphotransferase (pts) encoded by gfrabcd (glucoselysine/fructoselysine ...	2015	26100043
generating a metal-responsive transcriptional regulator to test what confers metal sensing in cells.	frmr from salmonella enterica serovar typhimurium (a csor/rcnr-like transcriptional de-repressor) is shown to repress the frmra operator-promoter, and repression is alleviated by formaldehyde but not manganese, iron, cobalt, nickel, copper, or zn(ii) within cells. in contrast, repression by a mutant frmre64h (which gains an rcnr metal ligand) is alleviated by cobalt and zn(ii). unexpectedly, frmr was found to already bind co(ii), zn(ii), and cu(i), and moreover metals, as well as formaldehyde, t ...	2015	26109070
salmonella typhimurium outbreak associated with a contaminated food container in a school in sichuan province, china.	on 13 june 2013, a fever and diarrhoea outbreak occurred in a boarding school in sichuan province. we conducted a field investigation and compared food exposure of 81 case students and 104 control students (years 7 and 8) in order to identify the source of infection. there were 401 cases identified (399 students and two cooks). the attack rates were 23-46% in nursery, primary, and secondary schools, but 0% in the high school. eighty-five percent of case students, consumed cowpea salad compared t ...	2016	26119077
quantification of viable but nonculturable salmonella spp. and shigella spp. during sludge anaerobic digestion and their reactivation during cake storage.	the presence of viable but nonculturable (vbnc) bacterial pathogens which often fail to be detected by cultivation and can regain the cultivability if the living conditions improve were reported. the objective of this study was to determine the occurrence of vbnc salmonella spp. and shigella spp. in the biosolids during anaerobic digestion and its reactivation during the cake storage.	2015	26119588
antimicrobial activity of customary medicinal plants of the yaegl aboriginal community of northern new south wales, australia: a preliminary study.	this study is a collaboration between macquarie university researchers and the yaegl aboriginal community of northern nsw, australia to investigate the antimicrobial potential of plants used in the topical treatment of wounds, sores and skin infections. based on previously documented medicinal applications, aqueous and aqueous ethanolic extracts of alocasia brisbanensis, canavalia rosea, corymbia intermedia, hibbertia scandens, ipomoea brasiliensis, lophostemon suaveolens and syncarpia glomulife ...	2015	26122212
a boundary value approach to optimization with an application to salmonella competition.	we develop a novel optimization framework to study strategies in ecological competition processes. the optimization method uses theory from dynamical systems describing the asymptotic behavior of a bistable system based on initial conditions, which we implement using a numerical boundary value problem. as an application of our method, we develop a model of the competition between salmonella typhimurium and the host's native microflora, which constantly and densely inhabit the intestinal lining o ...	2015	26122824
quantitative distribution of salmonella spp. and escherichia coli on beef carcasses and raw beef at retail establishments.	salmonella is a foodborne pathogen that commonly inhabits the gastrointestinal tract of a healthy feedlot cattle and can be transferred to the carcass surface during hide removal and evisceration procedures. numerous investigations on salmonella prevalence throughout different stages of the beef chain have been conducted. in contrast, limited studies are available on quantitative determinations of salmonella at different steps in raw meat production. quantitative data, particularly for pathogeni ...	2015	26125489
characterization of salmonella typhimurium isolates from domestically acquired infections in finland by phage typing, antimicrobial susceptibility testing, pfge and mlva.	salmonella enterica spp. enterica serotype typhimurium (stm) is the most common agent of domestically acquired salmonellosis in finland. subtyping methods which allow the characterization of stm are essential for effective laboratory-based stm surveillance and for recognition of outbreaks. this study describes the diversity of finnish stm isolates using phage typing, antimicrobial susceptible testing, pulsed-field gel electrophoresis (pfge) and multilocus variable-number tandem repeat analysis ( ...	2015	26129826
evaluation of synergistic antibacterial and antioxidant efficacy of essential oils of spices and herbs in combination.	the present study was carried out to evaluate the possible synergistic interactions on antibacterial and antioxidant efficacy of essential oils of some selected spices and herbs [bay leaf, black pepper, coriander (seed and leaf), cumin, garlic, ginger, mustard, onion and turmeric] in combination. antibacterial combination effect was evaluated against six important food-borne bacteria (bacillus cereus, listeria monocytogenes, micrococcus luteus, staphylococcus aureus, escherichia coli and salmone ...	2015	26132146
expression of the blood-group-related gene b4galnt2 alters susceptibility to salmonella infection.	glycans play important roles in host-microbe interactions. tissue-specific expression patterns of the blood group glycosyltransferase β-1,4-n-acetylgalactosaminyltransferase 2 (b4galnt2) are variable in wild mouse populations, and loss of b4galnt2 expression is associated with altered intestinal microbiota. we hypothesized that variation in b4galnt2 expression alters susceptibility to intestinal pathogens. to test this, we challenged mice genetically engineered to express different b4galnt2 tiss ...	2015	26133982
selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting.	the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ...	2014	26139877
electrospun antimicrobial hybrid mats: innovative packaging material for meat and meat-products.	to prevent the development and spread of spoilage/pathogenic microorganisms via meat foodstuffs, antimicrobial nanocomposite packaging can serve as a potential alternative. the objective of this study was to develop a new class of antimicrobial hybrid packaging mat composed of biodegradable polyurethane supplemented with virgin olive oil and zinc oxide via electrospinning. instead of mixing antimicrobial compounds directly with food, incorporation in packaging materials allows the functional eff ...	2015	26139931
in vitro probiotic characteristics of lactobacillus plantarum zdy 2013 and its modulatory effect on gut microbiota of mice.	lactobacillus plantarum zdy 2013, a novel strain isolated from chinese traditional fermented acid beans, was systematically evaluated for its survival capacity under stress conditions (ph, bile salt, simulated gastrointestinal tract, and antibiotics), production of exopolysaccharide and antagonism against 8 pathogens. its effect on mice gut microbiota was also investigated by quantitative pcr and pcr-denaturing gradient gel electrophoresis. the results showed that zdy 2013 can grow at ph 3.5 and ...	2015	26142853
identification of salmonella typhimurium deubiquitinase ssel substrates by immunoaffinity enrichment and quantitative proteomic analysis.	ubiquitination is a key protein post-translational modification that regulates many important cellular pathways and whose levels are regulated by equilibrium between the activities of ubiquitin ligases and deubiquitinases. here, we present a method to identify specific deubiquitinase substrates based on treatment of cell lysates with recombinant enzymes, immunoaffinity purification, and global quantitative proteomic analysis. as a model system to identify substrates, we used a virulence-related ...	2015	26147956
recombinant salmonella expressing burkholderia mallei lps o antigen provides protection in a murine model of melioidosis and glanders.	burkholderia pseudomallei and burkholderia mallei are the etiologic agents of melioidosis and glanders, respectively. these bacteria are highly infectious via the respiratory route and can cause severe and often fatal diseases in humans and animals. both species are considered potential agents of biological warfare; they are classified as category b priority pathogens. currently there are no human or veterinary vaccines available against these pathogens. consequently efforts are directed towards ...	2015	26148026
functional analysis of a novel cysteine-rich antimicrobial peptide from the salivary glands of the tick rhipicephalus haemaphysaloides.	ticks encounter various microbes while sucking blood from an infected host and carrying these pathogens in themselves. ticks can then transmit these pathogens to vertebrate hosts. the immune system of ticks can be stimulated to produce many bioactive molecules during feeding and pathogen invasion. antimicrobial peptides (amps) are key effector molecules of a tick's immune response, as they can kill invading pathogenic microorganisms. in this study, we identified a novel cysteine-rich amp, design ...	2015	26152423
stool bacteriomic profiling in patients with metastatic renal cell carcinoma receiving vascular endothelial growth factor-tyrosine kinase inhibitors.	diarrhea occurs in approximately half of patients with metastatic renal cell carcinoma (mrcc) receiving vascular endothelial growth factor-tyrosine kinase inhibitors (vegf-tki). we evaluated the relationship between vegf-tki-related diarrhea and stool microbiota.	2015	26152743
factors affecting pathogen survival in finished dairy compost with different particle sizes under greenhouse conditions.	this study investigated the survival of escherichia coli o157:h7 and salmonella typhimurium in finished dairy compost with different particle sizes during storage as affected by moisture content and temperature under greenhouse conditions. the mixture of e. coli o157:h7 and s. typhimurium strains was inoculated into the finished composts with moisture contents of 20, 30, and 40%, separately. the finished compost samples were then sieved into 3 different particle sizes (>1000, 500-1000, and <500 ...	2015	26153914
synthesis, in vitro and in silico studies of some novel 5-nitrofuran-2-yl hydrazones as antimicrobial and antitubercular agents.	in this study, we synthesized two series of novel 5-nitrofuran-2-carbohydrazides 21a-h and 22a-e in addition to a third series of thiophene-2-carbohydrazides 23a-g to develop potent antimicrobial and/or antitubercular agents. the newly synthesized compounds were evaluated in vitro for their antimicrobial and antimycobacterial activities. most of the 5-nitrofuran-2-carbohydrazides 21a-h and 22a-e displayed variable activity against aspergillus fumigates, staphylococcus aureus, streptococcus pneum ...	2015	26155871
efficacy of neutral ph electrolyzed water in reducing escherichia coli o157:h7 and salmonella typhimurium dt 104 on fresh produce items using an automated washer at simulated food service conditions.	the objective of this study was to determine the efficacy of neutral ph electrolyzed (neo) water (155 mg/l free chlorine, ph 7.5) in reducing escherichia coli o157:h7 and salmonella typhimurium dt 104 on romaine lettuce, iceberg lettuce, and tomatoes washed in an automated produce washer for different times and washing speeds. tomatoes and lettuce leaves were spot inoculated with 100 μl of a 5 strain cocktail mixture of either pathogen and washed with 10 or 8 l of neo water, respectively. washin ...	2015	26155998
attenuated mutant strain of salmonella typhimurium lacking the znuabc transporter contrasts tumor growth promoting anti-cancer immune response.	salmonella typhimurium has been shown to be highly effective as antitumor agent. the aim of this study was to investigate the tumor targeting efficacy and the mechanism of action of a specific attenuated mutant strain of salmonella typhimurium (stm) devoid of the whole operon coding for the high-affinity zinc transporter znuabc, which is required for bacterial growth in environments poor in zinc and for conferring full virulence to different gram-negative pathogens.we showed that stm is able to ...	2015	26158862
fundamental characteristics of deep-uv light-emitting diodes and their application to control foodborne pathogens.	low-pressure mercury uv (lp-uv) lamps have long been used for bacterial inactivation, but due to certain disadvantages, such as the possibility of mercury leakage, deep-uv-c light-emitting diodes (duv-leds) for disinfection have recently been of great interest as an alternative. therefore, in this study, we examined the basic spectral properties of duv-leds and the effects of uv-c irradiation for inactivating foodborne pathogens, including escherichia coli o157:h7, salmonella enterica serovar ty ...	2015	26162872
induction of immunocastration in pre-pubertal boars immunized with recombinant gonadotropin-releasing hormone conjugated with salmonella typhimurium flagellin fljb.	immunocastration is an alternative method used to replace surgical castration commonly performed in swine farms. in boars, the main effects of immunocastration are reduction of gonadotropin-releasing hormone (gnrh) and the resulting inhibition of testicular function. the aim of this study was to evaluate immunocastration efficacy in pre-pubertal boars vaccinated with a recombinant gnrh protein conjugated with salmonella typhimurium flagellin fljb (stf2). a total of 35 boars were assigned to thre ...	2015	26164876
plasma deposition of silver nanoparticles on ultrafiltration membranes: antibacterial and anti-biofouling properties.	a novel and versatile plasma reactor was used to modify polyethersulphone commercial membranes. the equipment was applied to: i) functionalize the membranes with low-temperature plasmas, ii) deposit a film of poly(methyl methacrylate) (pmma) by plasma enhanced chemical vapor deposition (pecvd) and, iii) deposit silver nanoparticles (snp) by gas flow sputtering. each modification process was performed in the same reactor consecutively, without exposure of the membranes to atmospheric air. scannin ...	2015	26166926
gastroprotective potential against indomethacin and safety assessment of the homology of medicine and food formula cuttlebone complex.	cuttlebone complex (cbc), a homology of medicine and food formula, is comprised of five herbal medicines (endoconcha sepiae, radix paeoniae rubra, fresh ginger, fructus amomi, and radix glycyrrhizae) and two food ingredients (zingiber zerumbet and chitosan). herein, the gastroprotective potential against indomethacin and a safety assessment of cbc were investigated. in a gastroprotective model, cbc effectively decreased the indomethacin-increased gastric ulcerous lesions, and increased the indom ...	2015	26169959
structural features reminiscent of atp-driven protein translocases are essential for the function of a type iii secretion-associated atpase.	many bacterial pathogens and symbionts utilize type iii secretion systems to interact with their hosts. these machines have evolved to deliver bacterial effector proteins into eukaryotic target cells to modulate a variety of cellular functions. one of the most conserved components of these systems is an atpase, which plays an essential role in the recognition and unfolding of proteins destined for secretion by the type iii pathway. here we show that structural features reminiscent of other atp-d ...	2015	26170413
nlrp3 inflammasome activation downstream of cytoplasmic lps recognition by both caspase-4 and caspase-5.	humans encode two inflammatory caspases that detect cytoplasmic lps, caspase-4 and caspase-5. when activated, these trigger pyroptotic cell death and caspase-1-dependent il-1β production; however the mechanism underlying this process is not yet confirmed. we now show that a specific nlrp3 inhibitor, mcc950, prevents caspase-4/5-dependent il-1β production elicited by transfected lps. given that both caspase-4 and caspase-5 can detect cytoplasmic lps, it is possible that these proteins exhibit som ...	2015	26173988
blue 470 nm light suppresses the growth of salmonella enterica and methicillin-resistant staphylococcus aureus (mrsa) in vitro.	emerging evidence suggests that blue light can photo-inactivate some bacteria of clinical importance. consequently, we tested the hypothesis that 470 nm light can suppress growth of two recalcitrant bacteria, mrsa and salmonella.	2015	26174877
evaluation of a water extract of so-cheong-ryong-tang for acute toxicity and genotoxicity using in vitro and in vivo tests.	so-cheong-ryong-tang, a traditional korean medicine, has been used empirically for the treatment of asthma, allergic rhinitis, and colds for hundreds of years. however, its genotoxicity has been rarely examined.	2015	26178777
understanding the complexities of salmonella-host crosstalk as revealed by in vivo model organisms.	foodborne infections caused by non-typhoidal salmonellae, such as salmonella enterica serovar typhimurium (st), pose a major challenge in the developed and developing world. with constant rise of drug-resistant strains, understanding the epidemiology, microbiology, pathogenesis and host-pathogen interactions biology is a mandatory requirement to enable health systems to be ready to combat these illnesses. patient data from hospitals, at least from some parts of the world, have aided in epidemiol ...	2015	26179888
laminarin from irish brown seaweeds ascophyllum nodosum and laminaria hyperborea: ultrasound assisted extraction, characterization and bioactivity.	ultrasound assisted extraction (uae), purification, characterization and antioxidant activity of laminarin from irish brown seaweeds ascophyllum nodosum and laminarina hyperborea were investigated. uae was carried out using 60% ultrasonic power amplitude and 0.1 m hydrochloric acid for 15 min. separately, solid-liquid extraction was carried in an orbital shaker using 0.1 m hydrochloric acid at 70 °c for 2.5 h. uae with hydrochloric acid resulted in the highest concentration of laminarin, 5.82% a ...	2015	26184235
from pasteur to probiotics: a historical overview of cheese and microbes.	cheese is a food which has been produced for centuries. while cheese was originally developed as a product which extended the shelf life of milk, over time distinct cheese varieties arose, being shaped by geographic, climate, cultural, and economic factors. global demand for artisan cheeses is creating new economic opportunities. consumers seeking distinctive products with regional flavor, or terroir, are becoming connoisseurs of hand-crafted cheeses with distinctive tastes and character. these ...	2013	26184821
correction: granulocytes impose a tight bottleneck upon the gut luminal pathogen population during salmonella typhimurium colitis.		2015	26186334
salmonella infection drives promiscuous b cell activation followed by extrafollicular affinity maturation.	the b cell response to salmonella typhimurium (stm) occurs massively at extrafollicular sites, without notable germinal centers (gcs). little is known in terms of its specificity. to expand the knowledge of antigen targets, we screened plasmablast (pb)-derived monoclonal antibodies (mabs) for salmonella specificity, using elisa, flow cytometry, and antigen microarray. only a small fraction (0.5%-2%) of the response appeared to be salmonella-specific. yet, infection of mice with limited b cell re ...	2015	26187411
crystal structure of β-n-acetylglucosaminidase cbsa from thermotoga neapolitana.	cbsa from the thermophilic marine bacteria thermotoga neapolitana is a chitinolyitc enzyme that can cleave a glycosidic bond of the polymer n-acetylglucosamine at the non-reducing end. this enzyme has particularly high activity on di-n-acetylchitobiose. cbsa consists of a family of 3 glycoside hydrolase (gh3)-type catalytic domains and a unique c-terminal domain. the c-terminal domain distinguishes cbsa from other gh3-type enzymes. sequence analyses have suggested that cbsa has the asp-his dyad ...	2015	26187666
cold plasma treatment for the microbiological safety of cabbage, lettuce, and dried figs.	microwave-powered cold plasma treatment (cpt) was evaluated as a means to improve the microbiological safety of fresh vegetables and dried fruits. the cpt at 900 w, conducted for 10 min using nitrogen as a plasma-forming gas, inactivated salmonella typhimurium inoculated on cabbage and lettuce by approximately 1.5 log cfu/g. the cpt at 400-900 w and 667 pa, conducted for 1-10 min using a helium-oxygen gas mixture, inactivated listeria monocytogenes on cabbage by 0.3-2.1 log cfu/g in a time-depen ...	2015	26187830
evaluation of in vitro and in vivo safety profile of the indian traditional medicinal plant grewia tiliaefolia.	grewia tiliaefolia vahl., is a subtropical tree used in ayurvedic formulations and also by tribal communities in india for treatment of wounds, urinary infection and skin diseases. despite of its medicinal use, the plant has not been previously tested for its safety. this study evaluated the toxicological profile of the methanolic extract of g. tiliaefolia leaves (megt) through in vitro (cytotoxic, mutagenic, genotoxic) and in vivo (acute and sub-acute) studies. in vitro assays conclude that the ...	2015	26188119
comparative cytotoxic and genotoxic potential of 13 drinking water disinfection by-products using a microplate-based cytotoxicity assay and a developed sos/umu assay.	the implications of disinfection by-products (dbps) present in drinking water are of public health concern because of their potential mutagenic, carcinogenic and other toxic effects on humans. in this study, we selected 13 main dbps found in drinking water to quantitatively analyse their cytotoxicity and genotoxicity using a microplate-based cytotoxicity assay and a developed sos/umu assay in salmonella typhimurium ta1535/psk1002. with the developed sos/umu test, eight dbps: 3-chloro-4-(dichloro ...	2016	26188195
microbiological toxicity of tilmicosin on human colonic microflora in chemostats.	to evaluate the microbiological safety of tilmicosin on human intestinal microflora, four chemostat models of healthy human colonic ecosystems were exposed to tilmicosin (0, 0.436, 4.36, and 43.6 μg/ml) for 7 days. prior to and during drug exposure, three microbiological endpoints were monitored daily including short-chain fatty acids, bacterial counts and macrolide susceptibility. colonization resistance of each community was determined by 3 successive daily challenges of salmonella typhimurium ...	2015	26190303
iron regulatory proteins mediate host resistance to salmonella infection.	macrophages are essential for systemic iron recycling, and also control iron availability to pathogens. iron metabolism in mammalian cells is orchestrated posttranscriptionally by iron-regulatory proteins (irp)-1 and -2. here, we generated mice with selective and combined ablation of both irps in macrophages to investigate the role of irps in controlling iron availability. these animals are hyperferritinemic but otherwise display normal clinical iron parameters. however, mutant mice rapidly succ ...	2015	26190773
characterization of salmonella enterica serovar 4,[5],12:i:- isolates from korean food animals.	salmonella enterica subspecies enterica serovar 4,[5],12:i:-, a monophasic variant of salmonella typhimurium, has emerged as one of the most common serotypes related to human salmonellosis. in this study, the 22 isolates of s. 4,[5],12:i:- from food animals were identified by a specific multiplex polymerase chain reaction between 2009 and 2012. the isolation rate of s. 4,[5],12:i:- accounted for 1.7% (22/1271) of salmonella spp. isolates from food animal origins: more specifically, 7.6% (18/235) ...	2015	26192872
inhibition of pathogenic bacterial growth on excision wound by green synthesized copper oxide nanoparticles leads to accelerated wound healing activity in wistar albino rats.	an impaired wound healing is one of the major health related problem in diabetic and non-diabetic patients around the globe. the pathogenic bacteria play a predominant role in delayed wound healing, owing to interaction in the wound area. in our previous work we developed green chemistry mediated copper oxide nanoparticles using ficus religiosa leaf extract. in the present study we make an attempt to evaluate the anti-bacterial, and wound healing activity of green synthesized copper oxide nanopa ...	2015	26194977
the o-antigen capsule of salmonella enterica serovar typhimurium facilitates serum resistance and surface expression of flic.	group iv polysaccharide capsules are common in enteric bacteria and have more recently been described in nontyphoidal salmonella species. such capsules are known as o-antigen (o-ag) capsules, due to their high degree of similarity to the o-ag of the lipopolysaccharide (lpso-ag). capsular polysaccharides are known virulence factors of many bacterial pathogens, facilitating evasion of immune recognition and systemic dissemination within the host. previous studies on the o-ag capsule of salmonellae ...	2015	26195553
efficacy of lytic bacteriophage preparation in reducing salmonella in vitro, on turkey breast cutlets, and on ground turkey.	the efficacy of the recently approved salmonella lytic bacteriophage preparation (salmofresh) in reducing salmonella enterica serotype heidelberg on turkey breast cutlets and ground turkey was evaluated. in a broth model assay, the phage preparation completely inhibited the growth of four s. enterica serotypes (salmonella enteritidis, salmonella heidelberg, salmonella kentucky, and salmonella typhimurium) at 37°c at a multiplicity of infection of 10,000 pfu/cfu. at 4°c in 0.1% peptone water (pw) ...	2015	26197288
microarray on digital versatile disc for identification and genotyping of salmonella and campylobacter in meat products.	highly portable, cost-effective, and rapid-response devices are required for the subtyping of the most frequent food-borne bacteria; thereby the sample rejection strategies and hygienization techniques along the food chain can be tailor-designed. here, a novel biosensor is presented for the generic detection of salmonella and campylobacter and the discrimination between their most prevalent serovars (salmonella enteritidis, salmonella typhimurium) and species (campylobacter jejuni, campylobacter ...	2015	26198111
rescuing chemotaxis of the anticancer agent salmonella enterica serovar typhimurium vnp20009.	the role of chemotaxis and motility in salmonella enterica serovar typhimurium tumor colonization remains unclear. we determined through swim plate assays that the well-established anticancer agent s. typhimurium vnp20009 is deficient in chemotaxis, and that this phenotype is suppressible. through genome sequencing, we revealed that vnp20009 and four selected suppressor mutants had a single nucleotide polymorphism (snp) in chey causing a mutation in the conserved proline residue at position 110. ...	2015	26200833
combination of dynamic magnetophoretic separation and stationary magnetic trap for highly sensitive and selective detection of salmonella typhimurium in complex matrix.	foodborne illnesses have always been a serious problem that threats public health, so it is necessary to develop a method that can detect the pathogens rapidly and sensitively. in this study, we designed a magnetic controlled microfluidic device which integrated the dynamic magnetophoretic separation and stationary magnetic trap together for sensitive and selective detection of salmonella typhimurium (s. typhimurium). coupled with immunomagnetic nanospheres (imns), this device could separate and ...	2015	26201979
salmonella typhimurium co-opts the host type i ifn system to restrict macrophage innate immune transcriptional responses selectively.	innate immune inflammatory responses are subject to complex layers of negative regulation at intestinal mucosal surfaces. although the type i ifn system is critical for amplifying antiviral immunity, it has been shown to play a homeostatic role in some models of autoimmune inflammation. type i ifn is triggered in the gut by select bacterial pathogens, but whether and how the type i ifn might regulate innate immunity in the intestinal environment have not been investigated in the context of salmo ...	2015	26202980
microencapsulated sorbic acid and pure botanicals affect salmonella typhimurium shedding in pigs: a close-up look from weaning to slaughter in controlled and field conditions.	the aim of this study was to assess the efficacy of a combination of sorbic acid, thymol, and carvacrol in reducing the prevalence and shedding level of salmonella typhimurium in pigs either in a controlled challenge environment or in a production setting. in the first study, 24 weaned piglets were separated in 4 isolation units (6 piglets/isolation unit). each unit received either a basal diet (no treatment) or a microencapsulated mixture of sorbic acid, thymol, and carvacrol at 1, 2, or 5 g/kg ...	2015	26203634