Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| genetic characterization of ovine herpesvirus 2 strains involved in water buffaloes malignant catarrhal fever outbreaks in southern italy. | ovine herpesvirus 2 (ovhv-2) was responsible for two outbreaks of malignant catarrhal fever (mcf) on two water buffalo farms in southern italy. in this study, the presence of this virus in the nasal swabs from sick animals as well as in the organs of dead buffaloes was ascertained by a real-time pcr assay. positive samples also underwent a relative quantitative analysis of the viral dna in them. all the dead animals had the highest relative viral quantities, while buffaloes recovering from the v ... | 2017 | 28110782 |
| identification of genetic variation in nod-like receptor 2 gene and influence of polymorphism on gene structure and function in buffalo (bubalus bubalis). | nucleotide-binding oligomerization domain (nod)-like receptor 2 is one of the important mediators of innate as well as adaptive immune response to microbial infections. in this study, nod-like receptor-2 was characterized by determining the full gene sequence and analyzing genetic diversity in indian buffaloes. sequence analysis of buffalo nod2 revealed 3042 nucleotides long orf, encoding 1013 amino acids from 12 exons. domain structure analysis indicated existence of 8 leucine-rich repeat (lrr) ... | 2017 | 28135669 |
| transcriptomic responses of water buffalo liver to infection with the digenetic fluke fasciola gigantica. | fasciola gigantica, the tropical liver fluke, infects buffaloes in asian and african countries and causes significant economic losses and poses public health threat in these countries. however, little is known of the transcriptional response of buffaloes to infection with f. gigantica. the objective of the present study was to perform the first transcriptomic analysis of buffalo liver infected by f. gigantica. understanding the mechanisms that underpin f. gigantica infection in buffaloes will co ... | 2017 | 28143561 |
| a brief and critical review on hydrofluorosis in diverse species of domestic animals in india. | india is one of the fluoride-endemic countries where the maximum numbers of ground or drinking water sources are naturally fluoridated. in india, a total of 23, out of 36 states and union territories have drinking water contaminated with fluoride in varying concentration. in the present scenario, especially in rural india, besides the surface waters (perennial ponds, dams, rivers, etc.), bore wells and hand pumps are the principal drinking water sources for domestic animals such as cattle (bos t ... | 2017 | 28144801 |
| comparative glycomics of fat globule membrane glycoconjugates from buffalo (bubalus bubalis) milk and colostrum. | the health-promoting effects of milk fat globule membrane (mfgm) glycoconjugates has attracted curiosity especially with regard to the challenges encountered to unravel the glycan complexities of mfgm glycoproteins and glycosphingolipids. in this context, we characterized glycans present in buffalo milk and colostrum fat globule membranes by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms analysis by adopting chemoselective glycoblotting technique. unlike human and bovi ... | 2017 | 28145111 |
| origins and global context of brucella abortus in italy. | brucellosis is a common and chronic disease of cattle and other bovids that often causes reproductive disorders. natural infection in cattle is caused by brucella abortus and transmission typically occurs during abortions, calving, or nursing. brucellosis is also a major zoonotic disease due to contamination of dairy products or contact with the tissues of infected animals. brucellosis has been eradicated from most of the developed world in the last 40 years but persists in many regions-the dise ... | 2017 | 28152976 |
| inhibition of snake venom metalloproteinase by β-lactoglobulin peptide from buffalo (bubalus bubalis) colostrum. | bioactive peptide research has experienced considerable therapeutic interest owing to varied physiological functions, efficacy in excretion, and tolerability of peptides. colostrum is a rich natural source of bioactive peptides with many properties elucidated such as anti-thrombotic, anti-hypertensive, opioid, immunomodulatory, etc. in this study, a variant peptide derived from β-lactoglobulin from buffalo colostrum was evaluated for the anti-ophidian property by targeting snake venom metallopro ... | 2017 | 28155167 |
| study of gastrointestinal parasites in water buffalo (bubalus bubalis) reared under mexican humid tropical conditions. | the objective was to determine the frequency of gastrointestinal parasites (gp) genera affecting water buffalo (bubalus bubalis) reared under humid tropical conditions of mexico. three hundred eighty-three munrah breed water buffalo were included, 251 adult females and 132 calves. feces were directly collected form the rectum of the animals and processed by the mcmaster technique. coprocultures were made to identify the genera of the nematodes. the frequency of gp in b. bubalis was 42%, independ ... | 2017 | 28161847 |
| urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed-artificial insemination in murrah buffalo (bubalus bubalis). | assessment of urine levels of luteinizing hormone (lh) for predicting the reproductive status of animals is in practice. the aim of this study was to predict the period of ovulation based on the urine levels of lh for timed-artificial insemination to increase the conception rate in buffaloes, which are naturally silent-oestrous animals. level of lh in urine was assessed using elisa, and a cut-off lh concentration for prediction of ovulation period was obtained using receiver operating characteri ... | 2017 | 28176388 |
| comparative study of the gut microbiome potentially related to milk protein in murrah buffaloes (bubalus bubalis) and chinese holstein cattle. | previous studies suggested a close relationship between ruminant gut microbes and the mammary gland. in this study, shotgun metagenomic sequencing was used to reveal the differences in the intestinal microbiome potentially related to milk components in murrah buffaloes and chinese holstein cattle. a pcoa based on the weighted unifrac distances showed an apparent clustering pattern in the structure of intestinal microbiota between buffalo and cattle. we could attribute the structural difference t ... | 2017 | 28176851 |
| congenital malformations in river buffalo (bubalus bubalis). | the world buffalo population is about 168 million, and it is still growing, in india, china, brazil, and italy. in these countries, buffalo genetic breeding programs have been performed for many decades. the occurrence of congenital malformations has caused a slowing of the genetic progress and economic loss for the breeders, due to the death of animals, or damage to their reproductive ability or failing of milk production. moreover, they cause animal welfare reduction because they can imply foe ... | 2017 | 28208595 |
| basic fibroblast growth factor is critical to reprogramming buffalo (bubalus bubalis) primordial germ cells into embryonic germ stem cell-like cells. | primordial germ cells (pgcs) are destined to form gametes in vivo, and they can be reprogrammed into pluripotent embryonic germ (eg) cells in vitro. buffalo pgc have been reported to be reprogrammed into eg-like cells, but the identities of the major signaling pathways and culture media involved in this derivation remain unclear. here, the effects of basic fibroblast growth factor (bfgf) and downstream signaling pathways on the reprogramming of buffalo pgcs into eg-like cells were investigated. ... | 2017 | 28215675 |
| cryoprotectant effect of trehalose in extender on post-thaw quality and in vivo fertility of water buffalo (bubalus bubalis) bull spermatozoa. | this study was designed to ascertain the cryoprotectant effects of different concentrations of trehalose [0 (t0), 25 (t25), 35 (t35), 45 (t45) mm], egg yolk [20% (e20), 15% (e15) v/v] and glycerol [7% (g7), 5% (g5) v/v] in tris-citric acid-based extender on post-thaw quality and in vivo fertility of buffalo bull spermatozoa. twenty-five ejaculates were collected from five bulls and split into four parts. after that, the split ejaculates from each of the bull were diluted either in t0e20g7 (contr ... | 2017 | 28224657 |
| extra and intracellular calcium signaling pathway(s) differentially regulate histamine-induced myometrial contractions during early and mid-pregnancy stages in buffaloes (bubalus bubalis). | this study examines the differential role of calcium signaling pathway(s) in histamine-induced uterotonic action during early and mid-pregnancy stages in buffaloes. compared to mid pregnancy, tonic contraction, amplitude and mean-integral tension were significantly increased by histamine to produce myometrial contraction during early pregnancy with small effects on phasic contraction and frequency. although uterotonic action of histamine during both stages of pregnancy is sensitive to nifedipine ... | 2017 | 28228338 |
| effect of feeding of blend of essential oils on methane production, growth, nutrient utilization in growing buffaloes. | an experiment was conducted to study the effect of a blend of essential oils (beo) on enteric methane emission and growth performance of buffaloes (bubalus bubalis). | 2017 | 28231698 |
| effect on quanti-quality milk and mozzarella cheese characteristics with further increasing the level of dried stoned olive pomace in diet for lactating buffalo. | following a previous report, an experiment was conducted to determine the effect of increasing level of dsop (dried stoned olives pomaces) in the diet of lactating buffaloes on milk and mozzarella cheese yield and characteristics. | 2017 | 28231700 |
| controlled breeding and reproductive management in water buffaloes (<i>bubalus bubalis</i>) using eazi breed controlled internal drug release. | buffalo reproduction is considerably affected by late maturity, poor oestrus symptoms and long postpartum periods. this study was undertaken to evaluate the efficiency of eazi breed controlled internal drug release (cidr), an intravaginal progesterone-releasing device, in relation to oestrus and fertility. five hundred true anoestrus buffalo cows, in the age group 4-6 years in 10 villages of dharwad district in karnataka state in india, were randomly selected and treated with cidr for 9 days. tw ... | 2015 | 28235292 |
| serum biochemical and haematological reference intervals for water buffalo (<i>bubalus bubalis</i>) heifers. | based on a review of the literature, reference intervals for water buffalo (bubalus bubalis) serum biochemistry and haematology have not previously been published. the current study was done to establish reference intervals for water buffalo heifers. the international federation of clinical chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. a total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the stud ... | 2014 | 28235298 |
| functional involvement of protein kinase c, rho-kinase and trpc3 decreases while plc increases with advancement of pregnancy in mediating oxytocin-induced myometrial contractions in water buffaloes (bubalus bubalis). | present study unravels the involvement of different calcium signaling pathways in oxytocin-induced contractions in myometrium of non-pregnant and pregnant buffaloes during early and mid-pregnancy stages. uteri of pregnant animals were more sensitive than of non-pregnant buffaloes. phasic contractions and frequency of contraction significantly increased with advancement of pregnancy, while tonic contractions non-significantly and amplitude significantly decreased from six months pregnancy onward. ... | 2017 | 28237334 |
| identification and evaluation of reference genes for accurate gene expression normalization of fresh and frozen-thawed spermatozoa of water buffalo (bubalus bubalis). | the quantitative real time pcr (qrt-pcr) has become an important tool for gene-expression analysis for a selected number of genes in life science. although large dynamic range, sensitivity and reproducibility of qrt-pcr is good, the reliability majorly depend on the selection of proper reference genes (rgs) employed for normalization. although, rgs expression has been reported to vary considerably within same cell type with different experimental treatments. no systematic study has been conducte ... | 2017 | 28237344 |
| functional gene profiling through metarnaseq approach reveals diet-dependent variation in rumen microbiota of buffalo (bubalus bubalis). | recent advances in next generation sequencing technology have enabled analysis of complex microbial community from genome to transcriptome level. in the present study, metatranscriptomic approach was applied to elucidate functionally active bacteria and their biological processes in rumen of buffalo (bubalus bubalis) adapted to different dietary treatments. buffaloes were adapted to a diet containing 50:50, 75:25 and 100:0 forage to concentrate ratio, each for 6 weeks, before ruminal content sam ... | 2017 | 28246035 |
| spermatozoa with high mitochondrial membrane potential and low tyrosine phosphorylation preferentially bind to oviduct explants in the water buffalo (bubalus bubalis). | although it is understood that spermatozoa are subjected to selection processes to form a functional sperm reservoir in the oviduct, the mechanism remains obscure. with the aim to understand the sperm selection process in the oviduct, in the present in vitro study, we analyzed mitochondrial membrane potential and tyrosine phosphorylation status in oviduct-explants bound and unbound spermatozoa. frozen semen from murrah buffalo bulls (n=10) used under progeny testing programme were utilized for t ... | 2017 | 28262463 |
| differential surface glycoprofile of buffalo bull spermatozoa during mating and non-mating periods. | the buffalo has a seasonal reproduction activity with mating and non-mating periods occurring from late autumn to winter and from late spring to beginning of autumn, respectively. sperm glycocalyx plays an important role in reproduction as it is the first interface between sperm and environment. semen quality is poorer during non-mating periods, so we aimed to evaluate if there were also seasonal differences in the surface glycosylation pattern of mating period spermatozoa (mps) compared with no ... | 2017 | 28264742 |
| 32 next-generation sequencing discloses differences in microrna expression profiles of buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization. | mammalian embryo development is a complex process with a series of critical events taking place at every stage of development. it is an established fact that the birth rate of animals produced by nuclear transfer (nt) is far less (<2%) than that of ivf embryos (40%) after successful embryo transfers in different farm animal species. micro(mi)rnas are small non-coding rnas of 17 to 25 nucleotides that alter the function of their target genes by either degrading them or inhibiting their expression ... | 2016 | 28278829 |
| 33 buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization differ in their global transcriptome profile. | although the blastocyst rate obtained with nuclear transferred (nt) embryos is higher than that obtained following in vitro fertilization (ivf) in buffalo, the live birth rate of nt embryos is <2% across different farm animal species compared with a birth rate >40% obtained with ivf embryos. this is believed to be due primarily to incomplete or incorrect nuclear reprogramming of the donor somatic cell by the oocyte, which results in aberrant embryonic gene expression. we compared the global tran ... | 2016 | 28278830 |
| 121 three-dimensional assessment of early corpus luteum vascularity in buffalo (bubalus bubalis). | the aim of the study was to develop an objective method to assess the vascular flow to the early corpus luteum (cl) in buffaloes using colour doppler ultrasound data. our hypothesis was that 3-dimensional (3d) volumetric analysis of vascularity would demonstrate lower variability between animals compared with conventional 2-dimensional (2d) analysis of single images. wave emergence and ovulation was synchronized in buffalo (n=16) using prostaglandin-gnrh based protocols. colour doppler ultrasono ... | 2016 | 28278918 |
| 151 effect of ascorbic acid on oxidative stress and its thermoprotectant role on in vitro embryonic development of buffalo (bubalus bubalis) embryos. | the most important factors that lead to stress in farm animals are oxidative and thermal stress, leading to reduced reproductive efficiency. oxidative stress leads to an increase in proportion of reactive oxygen species, whereas heat stress affects the physiology of animals, which lowers the conception rates of dairy cattle. in vitro culture systems have been enhanced by manipulating media with various supplements such as vitamins, growth factors, and antioxidants that have overcome these proble ... | 2016 | 28278948 |
| 164 carnitine improves post-thawing sperm motility by increasing adenosine triphosphate content in buffalo (bubalus bubalis). | semen cryopreservation plays a critical role for a wide application of both ai and in vitro embryo production in buffalo. in this species, spermatozoa are more susceptible to hazards during freezing and thawing than cattle spermatozoa, thus resulting in lower fertilizing potential (andrabi et al. 2008 anim. reprod. sci. 104, 427-433). carnitine is a quaternary ammonium compound with antioxidant capacities, able to reduce the availability of lipids for peroxidation by transporting fatty acids int ... | 2016 | 28278961 |
| 198 successful transplantation of buffalo (bubalus bubalis) germ cells to homologous recipients. | transplantation of isolated germ cells from a fertile donor male into the seminiferous tubules of infertile recipients can result in donor germ cells-derived sperm production. this technique has the potential to be used as an alternative strategy for producing transgenic livestock with higher efficiency and less time and capital requirement than the current methods. the objective of the present study was to investigate whether the homologous transplantation of isolated buffalo germ cells could r ... | 2016 | 28278995 |
| 208 production of transgenic cloned buffalo (bubalus bubalis) embryos containing human insulin gene through hand-guided cloning. | diabetes is a growing disease worldwide and has emerged as a major healthcare problem in india. insulin is an essential medicine for the treatment of diabetes. large dairy animals, such as buffaloes and cows, may be used as bioreactors for cost-effective production of human insulin. the present study was aimed to produce transgenic buffalo embryos containing the human insulin gene through hand-guided cloning for production of transgenic animals. buffalo female fetal fibroblast cells at passage n ... | 2016 | 28279005 |
| 209 production of buffalo (bubalus bubalis) embryos containing human lysozyme gene. | lysozyme is a ubiquitous enzyme found in all major taxa of living organisms with a diverse role in human and animal health. it plays an important role as a nonspecific immune factor and anti-inflammatory factor and is a part of the innate immune system. research on the expression of recombinant human lysozyme is, thus, potentially valuable to the dairy industry. therefore, the present study was carried out to observe the developmental competence and quality of cloned embryos containing the human ... | 2016 | 28279006 |
| changes in the total lipid, neutral lipid, phospholipid and fatty acid composition of phospholipid fractions during pastırma processing, a dry-cured meat product. | pastırma is a dry-cured meat product, produced from whole beef or water buffalo muscles. this study was carried out to investigate the effect of production stages (raw meat, after curing, after 2(nd) drying and pastırma) on the total lipid, neutral lipid, phospholipid and fatty acid composition of phospholipid fraction of pastırma produced from beef m. longissimus dorsi muscles. the ph and colour (l*, a* and b*) analyses were also performed in raw meat and pastırma. it was found that pastırma pr ... | 2017 | 28316467 |
| empirical and bioinformatic characterization of buffalo (bubalus bubalis) colostrum whey peptides & their angiotensin i-converting enzyme inhibition. | whey based peptides are well known for their nutritional and multifunctional properties. in this context, whey proteins from buffalo colostrum & milk were digested by in vitro simulation digestion and analyzed by nano-lc-ms/ms. functional protein association networks, gene annotations and localization of identified proteins were carried out. an ace inhibitory peptide sorted from the library was custom synthesized and an in vitro ace assay was performed. the study led to the identification of 74 ... | 2017 | 28317767 |
| relationship between mozzarella yield and milk composition, processing factors, and recovery of whey constituents. | our aim was to identify the relationship between mozzarella cheese yield and buffalo milk composition, processing factors, and recovery of whey constituents. a production of 30 batches of mozzarella cheese at a dairy industry in northeast brazil (rio grande do norte) was monitored between march and november, 2015. mozzarella yield and 32 other variables were observed for each batch, and divided into 3 groups: milk composition variables (12); variables involved in the cheesemaking process (14); a ... | 2017 | 28342605 |
| profile and artificial insemination practices of technicians and the artificial insemination success rates in leyte, samar, and biliran, philippines (2011-2015). | artificial insemination (ai) is a reproductive biotechnology that may be influenced by several factors, including the profile of the technicians and the practices used. assessing technician's profile and their ai practices can be significant in improving ai success rate. | 2017 | 28344401 |
| identification of single nucleotide polymorphism in protein phosphatase 1 regulatory subunit 11 gene in murrah bulls. | this study was conducted with the objective to identify single nucleotide polymorphism (snp) in protein phosphatase 1 regulatory subunit 11 (ppp1r11) gene in murrah bulls. | 2017 | 28344410 |
| molecular cloning, identification, and expression patterns of myostatin gene in water buffalo (bubalus bubalis). | myostatin (mstn), also named growth differentiation factor 8 (gdf8), is a transforming growth factor-β (tgf-β) family member with a key role in the negative regulation of skeletal muscle growth. however, its role in ovarian folliculogenesis remains unclear. to provide us with a basis for understanding this role, we cloned mstn and examined its expression patterns in water buffalo (bubalus bubalis). the complete orf of the water buffalo mstn gene is 1,128 nucleotides, which encode a 375 amino aci ... | 2017 | 28355117 |
| complement component 3: characterization and association with mastitis resistance in egyptian water buffalo and cattle. | mastitis is an infectious disease of the mammary gland that leads to reduced milk production and change in milk composition. complement component c3 plays a major role as a central molecule of the complement cascade involving in killing of microorganisms, either directly or in cooperation with phagocytic cells. c3 cdna were isolated, from egyptian buffalo and cattle, sequenced and characterized. the c3 cdna sequences of buffalo and cattle consist of 5025 and 5019 bp, respectively. buffalo and ca ... | 2017 | 28360391 |
| cryopreservation of nili-ravi buffalo bull sperm in cryodiluent supplemented with lolium perenne protein preparations. | semen from the nili-ravi buffalo bull, bubalus bubalis, shows poor survival after freeze storage compared to bovine (bos taurus and bos indicus) semen. freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes. | 2017 | 28376139 |
| comparative antinociceptive and sedative effects of epidural romifidine and detomidine in buffalo (bubalus bubalis). | in this study, comparative antinociceptive and sedative effects of epidural administration of romifidine and detomidine in buffalo were evaluated. eighteen healthy adult buffalo, allocated randomly in three groups (two experimental and one control; n=6) received either 50 μg/kg of romifidine or detomidine diluted in sterile saline (0.9 per cent) to a final volume of 20 ml, or an equivalent volume of sterile saline epidurally. antinociception, sedation and ataxia parameters were recorded immediat ... | 2017 | 28386033 |
| heterologous fibrin sealant derived from snake venom: from bench to bedside - an overview. | hemostatic and adhesive agents date back to world war ii, when homologous fibrin sealant came onto scene. considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. its components were a serine protease (a thrombin-like enzyme) extracted from the venom of crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of bubalus bubalis buffaloes. this new bioproduct has been used as ... | 2017 | 28396682 |
| lumpy skin disease in cattle: frequency of occurrence in a dairy farm and a preliminary assessment of its possible impact on egyptian buffaloes. | lumpy skin disease (lsd) is an endemic infectious disease of cattle in egypt. this survey aimed to define the prevalence of clinical and sub-clinical lsd virus (lsdv) infection among cattle and investigate their contact with water buffaloes (bubalus bubalis) in order to improve the understanding of lsd epidemiology. cattle and buffalo were examined owing to the appearance of skin lesions. because clinical signs were consistent with lsdv infection, samples from cattle in a non-grazing dairy farm ... | 2017 | 28397517 |
| intermolecular interactions between dmα and dmβ proteins in bula-dm complex of water buffalo bubalus bubalis. | the major histocompatibility complex (mhc) with its three classes represents a cluster of tightly linked genes with defined immunological and non-immunological functions. the dm, a mhc class ii molecule is formed by the non-covalent association of dmα and dmβ chains. it binds with the processed peptide antigens and presents them to t lymphocytes, thereby triggering the immune responses. startlingly, the expression pattern and structural organization of dmα and dmβ proteins in buffalo remains und ... | 2017 | 28419545 |
| characterization of leukocyte subsets in buffalo (bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine mhc class i and class ii molecules and leukocyte differentiation molecules. | although buffaloes (bubalus bubalis) are a major component of the livestock industry worldwide, limited progress has been made in the study of the mechanisms regulating the immune response to pathogens and parasites affecting their health and productivity. this has been, in part, attributable to the limited availability of reagents to study immune responses in buffalo. as reported here, a set of cross-reactive monoclonal antibodies (mabs), developed against bovine, ovine and caprine leukocyte di ... | 2017 | 28433527 |
| buffalo leukemia inhibitory factor induces differentiation and dome-like secondary structures in cos-1 cells. | this study aimed to understand the molecular characteristics of buffalo leukemia inhibitory factor (bulif) and the generation of a stably transfected cos-1_bulif cell line for its functional characterization. cumulus cells, isolated from oocytes, were separated, and total cdna was prepared. the bulif gene was ligated into the cloning vector pjet1.2/blunt and expression vector pacgfp-n1 which was transfected into cos-1 cells and confirmed by qrt-pcr and western blot. bulif was immunoprecipitated ... | 2017 | 28441662 |
| mastitis outcomes on pre-ovulatory follicle diameter, estradiol concentrations, subsequent luteal profiles and conception rate in buffaloes. | the objectives of this study was to investigate the outcome of mastitis, in its clinical or subclinical forms, on the mean diameter of pre-ovulatory follicle (pof), plasma estradiol concentration on the day of estrus, subsequent luteal profile and subsequent conception rate in buffaloes. sixty dairy buffalo (bubalus bubalus) conducted in this study were divided into three groups {healthy (h), n=20; subclinical mastitis (scm), n=18; and clinical mastitis (cm), n=22}. ultrasonography of ovaries re ... | 2017 | 28442176 |
| plasma proteome of buffaloes. | the proteomic approach has aroused the interest of veterinary medicine researchers, especially regarding the production of biopharmaceuticals and diagnosis of diseases in farm animals. water buffaloes have gained prominence in the world economy due to the quality of their milk, meat, and leather, in addition to being an important donor of blood components. this work aimed to identify and characterize the proteins present in the blood plasma of murrah buffaloes (bubalus bubalis) through 2d electr ... | 2017 | 28452126 |
| ovulatory and fertility response using modified heatsynch and ovsynch protocols in the anovular murrah buffalo (bubalus bubalis). | we studied the effect of modified heatsynch and ovsynch protocols on the ovulatory response (or), estrus induction rate (eir) and conception rate (cr) in the anovular postpartum murrah buffalo (n = 35). in the modified heatsynch protocol (group i; n = 12), buffaloes were given two gnrh at 2 h interval on treatment day 0, pgf (pgf2α) on day 7 and estradiol (e2) 1 mg on day 8. two ftai were done at 20 h intervals after e2 administration. in the modified ovsynch protocol (group ii; n = 15), gnrh wa ... | 2017 | 28460685 |
| effect of sexual excitation on testosterone and nitric oxide levels of water buffalo bulls (bubalus bubalis) with different categories of sexual behavior and their correlation with each other. | we studied the effect of sexual excitation on serum testosterone and nitric oxide (no) levels in water buffalo bulls with different categories of sexual behavior and their correlation with each other. buffalo bulls were classified according to their sexual behavior (including reaction time, sexual aggressiveness and mating ability): acceptable (good to excellent) (n=5), fair (n=5), and unacceptable (poor) (n=5) sexual behavior. blood samples were collected from all animals immediately before and ... | 2017 | 28477950 |
| posttranslational modifications in spermatozoa and effects on male fertility and sperm viability. | spermatogenesis is a complex and highly regulated process. the ability of spermatozoa to perform its function depends on multiple physiological and genetic factors that are not fully understood. notably, due to lack of transcriptional and translational activity in spermatozoa, posttranslational modifications (ptms) play key roles in determining their viability. ptms not only confer structural changes in the proteome of the spermatozoa cells, but also increase the diversity of the proteome and in ... | 2017 | 28481731 |
| comparison of three different extenders on murrah buffaloes (bubalus bubalis) semen freezability. | the use of frozen semen for artificial insemination is the main approach utilised for the genetic improvement of most domesticated species. the advantages include lower transportation costs, continuous availability of semen, fewer occurrences of sexually transmitted diseases and the incorporation of desirable genes in a relatively short amount of time. nevertheless, the use of frozen semen in buffalo herds remains limited due to the loss of sperm quality when buffalo semen is frozen. so, the goa ... | 2018 | 28488738 |
| testicular thermoregulation, scrotal surface temperature patterns and semen quality of water buffalo bulls reared in a tropical climate. | this study evaluated the capacity of thermoregulation and its consequences on the scrotal surface temperature patterns and semen quality of buffalo bulls raised in a wet tropical climate. eleven water buffaloes were evaluated in the rainiest, in the transitional and in the less rainy season. air temperature and humidity were consistently high, but the animals did not show thermal stress in any season. the scrotal temperature gradient of buffalo bulls using infrared thermography was described, an ... | 2017 | 28517368 |
| a novel polymorphism in the oxytocin receptor encoding gene (oxtr) affects milk fatty acid composition in italian mediterranean river buffalo. | the oxytocin receptor, also known as oxtr, is a protein which functions as receptor for the hormone and neurotransmitter oxytocin and the complex oxytocin-oxytocin receptor plays an important role in the uterus during calving. a characterisation of the river buffalo oxtr gene, amino acid sequences and phylogenetic analysis is presented. the dna regions of the oxtr gene spanning exons 1, 2 and 3 of ten mediterranean river buffalo dna samples were analysed and 7 single nucleotide polymorphisms wer ... | 2017 | 28524009 |
| retinoic acid induces differentiation of buffalo (bubalus bubalis) embryonic stem cells into germ cells. | development of precise and reproducible culture system for in vitro differentiation of embryonic stem (es) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (pgcs), but in finally treating the catastrophic infertility. taking into account the significant role of retinoic acid (ra) during in vivo gametogenesis, we designed the present study to invest ... | 2017 | 28526652 |
| trace elements in raw milk of buffaloes (bubalus bubalis) from campania, italy. | the profile of 18 trace elements was traced in 68 milk samples collected from buffalo farms in the territory known as the "land of fires" in the campania region (italy). this area has been polluted by the illegal dumping in fields of industrial or domestic waste, wich is sometimes then burned spreading toxic contaminants. milk from buffaloes raised on rural farms might be a good indicator of environmental contamination risk in the human food chain. trace element analysis in milk was performed us ... | 2017 | 28530587 |
| transient arrest of germinal vesicle breakdown improved in vitro development potential of buffalo (bubalus bubalis) oocytes. | germinal vesicle breakdown (gvbd) is the first milestone that an oocyte needs to achieve toward completing the maturation and gaining potential to fertilize. significantly lower in vitro embryo production rate in buffaloes can be attributed to heterogeneity of gvbd occurrence among oocytes obtained from abattoir derived ovaries. evidence from our earlier work had suggested that different qualities of buffalo oocytes differ significantly in their timing of gvbd. besides, these oocytes also differ ... | 2018 | 28543358 |
| detection and localization of regucalcin in spermatozoa of water buffalo (bubalus bubalis): a calcium-regulating multifunctional protein. | regucalcin (rgn) is a calcium-regulating, anti-apoptotic, antioxidative and antiproliferative multifunctional protein predominantly seen in liver and kidney. all these functions are very crucial during spermatogenesis and sperm maturation process until fertilization of the ovum. although many studies have reported the wide distribution of regucalcin in the male reproductive tract of the rat, human and bovine, its presence in spermatozoa is yet to be demonstrated wherein calcium has a pivotal rol ... | 2017 | 28543743 |
| comparison of two methods of seminal plasma removal on buffalo (bubalus bubalis) sperm cryopreservation. | cryopreservation causes damage to spermatozoa, and methods minimizing this damage are therefore needed. although much discussed, seminal plasma removal has become an alternative to improve sperm quality and viability after freezing and has been applied to different species in attempt to obtain good results. the objective of this study was to evaluate semen quality in buffaloes submitted to two methods for seminal plasma removal (filtration and centrifugation). semen samples were collected from s ... | 2017 | 28543808 |
| fixed differences in the 3'utr of buffalo prnp gene provide binding sites for mirnas post-transcriptional regulation. | bovine spongiform encephalopathy, a member of transmissible spongiform encephalopathies, has not been reported in buffaloes, bubalus bubalis. prion protein (prp), encoded by the prion protein gene (prnp), is fundamental in the pathogenesis of transmissible spongiform encephalopathies. we previously showed that buffaloes express more prp proteins but lower prnp mrna than cattle in several pivotal tissues like the obex. therefore, we sought to establish whether genetic variability in prnp 3'utr, m ... | 2017 | 28545018 |
| characteristics of yak platelet derived growth factors-alpha gene and expression in brain tissues. | platelet derived growth factors (pdgfs) are key components of autocrine and paracrine signaling, both of which play important roles in mammalian developmental processes. pdgf expression levels also relate to oxygen levels. the characteristics of yak pdgfs, which are indigenous to hypoxic environments, have not been clearly described until the current study. | 2017 | 28553854 |
| sarcocystis dehongensis n. sp. (apicomplexa: sarcocystidae) from water buffalo (bubalus bubalis) in china. | water buffalo (bubalus bubalis) is the intermediate host for at least four species of sarcocystis: s. fusiformis, s. buffalonis, s. levinei, and s. sinensis/s. dubeyi. here, a new species, sarcocystis dehongensis, is reported in 51 of 756 (6.7%) water buffaloes in china. by light microscopy, the cysts of s. dehongensis were macroscopic, up to 18.5 mm long and 95 μm in diameter; 4.9-11.9 μm villous protrusions extended beyond the sarcocyst wall. using transmission electron microscopy, the sarcocy ... | 2017 | 28560573 |
| comprehensive characterization of bioactive peptides from buffalo (bubalus bubalis) colostrum and milk fat globule membrane proteins. | milk fat is dispersed in milk as small spherical globules stabilized in the form of emulsion by its surrounding membrane, often referred to as fat globule membrane (fgm). buffalo, a major milking mammal of asia and second most milking mammal across the globe presents physicochemical features different from that of other ruminant species containing higher content of lipids and proteins. the present study describes characterization of fgm proteins isolated from both buffalo milk and colostrum. a d ... | 2017 | 28578070 |
| hematologic ris for healthy water buffaloes (bubalus bubalis) in southern mexico. | baseline hematologic data are used by veterinarians and wildlife biologists to perform health assessments on target animal species. hematologic measurements are influenced by various factors including geography. baseline hematologic ris have been established for domestic water buffaloes (bubalus bubalis) from various countries in the eastern hemisphere, but these data are not readily available for the western hemisphere. | 2017 | 28582601 |
| pregnancy-associated glycoproteins (pags) concentrations in water buffaloes (bubalus bubalis) during gestation and the postpartum period. | for the first time in literature this study describes the pregnancy-associated glycoprotein (pag) profile of buffalo cows during gestation and the post-partum period using antiserum raised against pag-molecules purified from buffalo placenta (as#860). ninety-eight buffalo cows, belonging to a buffalo herd subjected to a synchronization and artificial insemination (ai) program, were enrolled in this study. blood samples were taken on days 0 (ai), 23, 25, 28, 30 and then biweekly until the end of ... | 2017 | 28583611 |
| prevalence and microscopic studies of sarcocystis infection in naturally infected water buffaloes (bubalus bubalis) of andhra pradesh. | sarcocystis species are the obligate tissue protozoan parasites of livestock causing clinical and subclinical disease resulting in downgrading of the meat and their products thereby leading to economic losses. the present study reveals the prevalence and distribution of sarcocystosis in water buffaloes (bubalus bubalis) slaughtered at local abattoirs in a.p for a period of 1 year from june 2014 to may 2015. a total of 137 buffalo carcasses were screened grossly and microscopically organ wise viz ... | 2017 | 28615863 |
| pcr-sscp analysis of mdgi gene and its association with milk production traits in river buffalo (bubalus bubalis). | in this study, we investigated the genetic variation within 3'utr of mammary-derived growth inhibitor (mdgi) gene of buffalo using pcr-sscp and sequencing; and also analyzed association of polymorphism with the milk production traits. the study revealed two conformational patterns, 'a' and 'b' among 234 mehsana buffaloes maintained with their records in the field and at farm. the frequency of sscp variant 'a' was found to be invariably high in the buffalo population under study. further, associa ... | 2017 | 28647599 |
| expression, localization of sumo-1, and analyses of potential sumoylated proteins in bubalus bubalis spermatozoa. | mature spermatozoa have highly condensed dna that is essentially silent both transcriptionally and translationally. therefore, post translational modifications are very important for regulating sperm motility, morphology, and for male fertility in general. protein sumoylation was recently demonstrated in human and rodent spermatozoa, with potential consequences for sperm motility and dna integrity. we examined the expression and localization of small ubiquitin-related modifier-1 (sumo-1) in the ... | 2017 | 28659810 |
| cryopreservation of nili-ravi buffalo (bubalus bubalis) semen in andromed® extender; in vitro and in vivo evaluation. | the study was designed to evaluate andromed® for the freezability and fertility of nili-ravi buffalo semen. semen was collected from four adult nili-ravi buffalo (bubalus bubalis) bulls for 3 weeks (replicate). semen ejaculates from each buffalo bull were divided into three aliquots. one aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, dna integrity and normal apical ridge. remaining two aliquots were diluted (37°c; 50 × 106 spermatozoa/ml) in tris-c ... | 2017 | 28660630 |
| ovum pick-up interval in buffalo (bubalus bubalis) managed under wetland conditions in argentina: effect on follicular population, oocyte recovery, and in vitro embryo development. | the excellent adaptation of water buffalo (bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. the objective of this study was to evaluate the effect of the ovum pick-up (opu) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. twelve murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or ... | 2017 | 28662882 |
| comparative efficacy of oestrus synchronization protocols in buffalo (bubalus bubalis). | this study was conducted to test the efficacy of gonadotropic hormone (gnrh)-based synchronization protocols (ovsynch, heatsynch, and ovsynch plus) in buffaloes under field condition. based on anamnesis and transrectal palpation twice at 10-day interval and serum progesterone (p4) concentration, 150 anoestrous buffaloes and delayed pubertal heifers were selected to induce oestrus using gnrh-based protocols. these selected animals were randomly divided into three groups: group i: ovsynch (n = 50) ... | 2017 | 28669062 |
| serological survey of neospora caninum and toxoplasma gondii in cattle (bos indicus) and water buffaloes (bubalus bubalis) in ten provinces of brazil. | the objective of this study was to determine the prevalence of antibodies to neospora caninum and toxoplasma gondii among 500 cattle (bos indicus) and 500 buffaloes (bubalus bubalis) using the indirect fluorescent antibody test (ifat) technique. blood samples from were collected from water buffalo and cattle in 10 municipalities in the northern region of brazil. the frequency of cattle and water buffaloes seropositive for neospora caninum in pará state, brazil, was 55% and 44%, respectively, and ... | 2017 | 28673459 |
| whole mitogenomes reveal the history of swamp buffalo: initially shaped by glacial periods and eventually modelled by domestication. | the newly sequenced mitochondrial genomes of 107 asian swamp buffalo (bubalus bubalis carabensis) allowed the reconstruction of the matrilineal divergence since ~900 kya. phylogenetic trees and bayesian skyline plots suggest a role of the glacial periods in the demographic history of swamp buffalo. the ancestral swamp-buffalo mitogenome is dated ~232 ± 35 kya. two major macro-lineages diverged during the 2nd pleistocene glacial period (~200-130 kya), but most (~99%) of the current matrilines der ... | 2017 | 28680070 |
| ethnomedicinal and cultural practices of mammals and birds in the vicinity of river chenab, punjab-pakistan. | although, use of animal species in disease treatment and culture practices is as ancient as that of plant species; however ethnomedicinal uses and cultural values of animal species have rarely been reported. present study is the first report on the medicinal uses of mammals and bird species in pakistan. | 2017 | 28701165 |
| expression and molecular cloning of interferon stimulated genes in buffalo (bubalus bubalis). | buffalo, the most important livestock species in tropical india, remains to be a poor breeder mainly due to embryonic mortality (65%) occurring mostly between 16 and 18 days of pregnancy. early and accurate diagnosis of pregnancy can thus become a boon for successful herd management in buffalo. however, most of the currently available methods allow diagnosis only after 30 days post ai. interferon tau (ifnt), the first pregnancy recognition signal in ruminants is one such molecule, which stimulat ... | 2017 | 28708533 |
| ultrastructure changes in buffalo (bubalus bubalis) oocytes before and after maturation in vitro with sericin. | the aim of this research was to identify the changes in the cytoplasmic ultrastructure of immature and matured oocytes in buffalo (bubalus bubalis). oocytes were matured in vitro in tissue culture medium-199 with and without sericin, and then analyzed by light and transmission electron microscopy. the experiment result showed that the nuclear maturation rate of buffalo oocytes was significantly higher in the presence of sericin (80.6%) than without sericin (68.1%) (p < 0.05). the immature oocyte ... | 2017 | 28722264 |
| influence of body condition score at calving on the metabolic status and production performance of murrah buffaloes (bubalus bubalis) during the transition period. | the purpose of this study was to evaluate the influence of body condition score (bcs) at calving on the metabolic status of female murrah buffaloes in the transition period. | 2017 | 28728402 |
| genomic differentiation between swamp and river buffalo using a cattle high-density single nucleotide polymorphisms panel. | buffalo (bubalus bubalis) is an important livestock species in many tropical and subtropical regions. in recent decades, the interest in buffalo's milk have expanded and intensive buffalo farms start to emerge. however, breeding programs and population genetics information for this species is scarce or inexistent. the present study aims to test the suitability of the commercial high-density single nucleotide polymorphisms (snp) genotyping panel, the illumina bovinehd beadchip, to estimate popula ... | 2017 | 28735584 |
| retinoic acid induces differentiation of buffalo (bubalus bubalis) embryonic stem cells into germ cells. | development of precise and reproducible culture system for in vitro differentiation of embryonic stem (es) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by limited availability of precursor primordial germ cells (pgcs), but in finally treating the catastrophic infertility. taking into account the significant role of retinoic acid (ra) during in vivo gametogenesis, we designed the present study to invest ... | 2017 | 28736154 |
| in-gel and offgel-based proteomic approach for authentication of meat species from minced meat and meat products. | fraudulent mislabelling of processed meat products on a global scale that cannot be detected using conventional techniques necessitates sensitive, robust and accurate methods of meat authentication to ensure food safety and public health. in the present study, we developed an in-gel (two-dimensional gel electrophoresis, 2de) and offgel-based proteomic method for authenticating raw and cooked water buffalo (bubalus bubalis), sheep (ovis aries) and goat (caprus hircus) meat and their mixes. | 2018 | 28737240 |
| determination of an optimal membrane-permeable cryoprotectant addition and dilution protocol for water buffalo spermatozoa. | there is a possibility to reduce the toxicity of glycerol and osmotic stress of dmso by lowering their concentrations in freezing extenders. | 2017 | 28767747 |
| changes in some blood parameters, milk composition and yield of buffaloes (bubalus bubalis) during the transition period. | in this study changes in hematochemical parameters, milk composition and yield were investigated in buffaloes during the transition period. a total of 93 buffaloes 113.9 ± 8.03 months old and 535 ± 50 kg average body weight were used. parity was recorded, blood samples were collected from 80 days pre-partum until 70 days post-partum; milk samples were collected from 5 days to 70 days post-partum. on serum samples, the values of non-esterified fatty acids, β-hydroxybutyrate, glucose, cholesterol, ... | 2017 | 28776872 |
| transcript analysis at dgat1 reveals different mrna profiles in river buffaloes with extreme phenotypes for milk fat. | buffalo dgat1 (diacylglycerol o-acyltransferase 1) was mainly investigated for the characterization of the gene itself and for the identification of the k232a polymorphism, similar to what has been accomplished in cattle, although no information has been reported so far at the mrna level. the importance of dgat1 for lipid metabolism led us to investigate the transcript profiles of lactating buffaloes characterized as high (9.13 ± 0.23) and low (7.94 ± 0.29) for milk fat percentage, and to explor ... | 2017 | 28780112 |
| effect of supplementation of recombinant regucalcin in extender on cryopreservation of spermatozoa of water buffalo (bubalus bubalis). | elevated intracellular calcium concentration and oxidative damage are two major factors contributing to the poor fertility of cryopreserved spermatozoa. regucalcin (rgn), also known as senescence marker protein-30 (smp-30), is a calcium-binding protein with multiple roles that include calcium homeostasis, anti-oxidative, anti-apoptosis, and anti-proliferation. in drosophila, rgn is reportedly a putative cold-tolerance gene and a cytoprotective role for rgn against intracellular calcium elevation ... | 2017 | 28782859 |
| influence of season, age and management on scrotal thermal profile in murrah bulls using scrotal infrared digital thermography. | the aim of the present study was to examine the effects of non-genetic factors on scrotal thermographic profile viz., proximal pole temperature (ppt °c), mid pole temperature (mpt °c), distal pole temperature (dpt °c) and ocular temperature (oct) of murrah (bubalus bubalis) breeding bulls. a total of 109 buffalo bulls, maintained at three semen stations (ss), were monitored for scrotal surface and ocular temperatures using infrared thermography twice daily during rainy, winter and summer seasons ... | 2017 | 28791483 |
| molecular characterization and comparison of phospholipase c zeta (plcz1) gene between swamp (bubalus carabanensis) and riverine (bubalus bubalis) buffaloes: its implications and future perspectives. | phospholipase c zeta, a novel sperm-specific protein which is widely known to induce oocyte activation following fertilization, had already been characterized in various mammalian species, but not in water buffaloes thus far. the present study was conducted to initially characterize and compare the sequences of plcz1 gene of swamp and riverine buffaloes. semen samples were collected; total rna was extracted and reverse-transcribed. plcz1 cdna was then amplified, and submitted for sequencing. buf ... | 2017 | 28799828 |
| evaluation of α-linolenic acid for freezability and in vivo fertility of nili ravi (bubalus bubalis) buffalo semen. | alpha linolenic acid (ala) is integral component of cell membrane that protects the cell in stressful events and involves in many metabolic pathways. it was hypothesized that ala have the ability to protect the structural and functional integrity of buffalo spermatozoa during freeze-thawing. therefore, study was designed to evaluate ala supplementation (0, 5, 10 and 20 ng/ml) in extender on freezability and in vivo fertility of buffalo bull spermatozoa. semen from three adult nili-ravi buffalo b ... | 2017 | 28802112 |
| biochemical analysis of uterine fluid for identification of indicators for subclinical endometritis in the water buffalo (bubalus bubalis). | alterations in biochemical constituents of uterine fluid have been suggested for diagnosis of subclinical uterine infection in the bovine. this study was undertaken to investigate whether uterine fluid biomolecules could act as tool for diagnosis of subclinical endometritis in the buffalo. uterine fluid samples from normal (n = 22) and subclinical endometritis (n = 18; diagnosed based on uterine cytology)-affected buffaloes were subjected to biochemical analysis. among the different biochemical ... | 2018 | 28804962 |
| surveillance for hemorrhagic septicemia in buffalo (bubalus bubalis) as an aid to range expansion of the javan rhinoceros (rhinoceros sondaicus) in ujung kulon national park, indonesia. | the javan rhinoceros (rhinoceros sondaicus) of ujung kulon national park (uknp) is the crown jewel of indonesia's rich natural history. the park lies on a peninsula surrounded by coastline and agriculture-dominated landscapes. the invasion of water buffalo (bubalus bubalis) into the park carries a substantial health risk to the javan rhinoceros and threatens plans to establish a new population outside of its only current range in uknp. hemorrhagic septicemia (hs), known locally as septicemia epi ... | 2017 | 28820298 |
| serotyping, antibiotic susceptibility, and virulence genes screening of escherichia coli isolates obtained from diarrheic buffalo calves in egyptian farms. | in egypt as in many other countries, river water buffalo (bubalus bubalis) is considered an important source of high-quality milk and meat supply. the objective of this study was to investigate serotypes, virulence genes, and antibiotic resistance determinants profiles of escherichia coli isolated from buffalo at some places in egypt; noticibly, this issue was not discussed in the country yet. | 2017 | 28831220 |
| comparative therapeutic effect of steroidal and non-steroidal anti-inflammatory drugs on pro-inflammatory cytokine production in water buffalo calves (bubalus bubalis) naturally infected with bronchopneumonia: a randomized clinical trial. | in the current study, we compared the therapeutic effects of a non-steroidal and a steroidal anti-inflammatory drug on the production of pro-inflammatory cytokines, interleukin-1β (il-1β), interleukin-6 (il-6), interleukin-12p40 (il-12p40), interferon gamma (ifnγ), and tumor necrosis factor alpha (tnf-α) in the blood of water buffalo (bubalus bubalis) calves naturally infected by bronchopneumonia. twenty-seven buffalo calves (7 ± 2-month-old, 163 ± 12 kg) reared in smallholder farms in el-dakahl ... | 2017 | 28831754 |
| analysis of microsatellite markers in a cuban water buffalo breed. | the aim of this regional research communication was to validate a panel of 30 microsatellite markers recommended by fao/isag for studies of biodiversity in cattle to improve the characterisation of cuban buffalo populations. the water buffalo (bubalus bubalis) is an economically important livestock species. therefore, research focused on the study of the genetic relationships among water buffalo populations is useful to support conservation decisions and to design breeding schemes. twenty-eight ... | 2017 | 28831973 |
| morphological studies of the tongue of the egyptian water buffalo (bubalus bubalis) and their lingual papillae adaptation for its feeding habits. | this work was conducted to describe the morphological characters of the tongue of egyptian water buffalo (bubalus bubalis). the lingual root and the dorsal middle region of apex and body in addition to the dorsal and ventral surface of lingual tip were devoided from any fungiform papillae. the lingual tip contains conical papillae only. the ventral surface of lingual apex was divided into two portions by the u-shaped fungiform line into papillary and non-papillary region. histological investigat ... | 2017 | 28833390 |
| biocomputational identification and validation of novel micrornas predicted from bubaline whole genome shotgun sequences. | micrornas (mirnas) are small (19-25 base long), non-coding rnas that regulate post-transcriptional gene expression by cleaving targeted mrnas in several eukaryotes. the mirnas play vital roles in multiple biological and metabolic processes, including developmental timing, signal transduction, cell maintenance and differentiation, diseases and cancers. experimental identification of micrornas is expensive and lab-intensive. alternatively, computational approaches for predicting putative mirnas fr ... | 2017 | 28844020 |
| the first serological evidence for rift valley fever infection in the camel, goitered gazelle and anatolian water buffaloes in turkey. | rift valley fever (rvf), a vector-borne zoonotic disease, is caused by a phlebovirus (family bunyaviridae). the virus was initially characterized approximately 80 years ago in kenya and disseminated to many countries in the continental africa, saudi arabia, and yemen. the infection has not been reported in turkey. in this study, blood serum samples collected from camel (camelus dromedairus), goitered gazelle (gazella subgutturosa subgutturosa), and buffaloes (bubalus bubalis linneaus) from 2000 ... | 2017 | 28861841 |
| anandamide exerts a suppressive effect on sperm binding to oviduct explants through cb1 receptors in the water buffalo (bubalus bubalis). | an endocannabinoid system comprising of anandamide (aea) and its receptor has been shown to play a role in sperm acquisition of fertilizing potential and sperm-oviduct interaction. in the present study, we assessed the effect of sperm pre-treatment with aea or co-incubation of sperm-oviduct explants with aea in the presence or absence of cb1 receptor antagonist (sr141716a) on sperm-oviduct binding in the water buffalo. cryopreserved spermatozoa from 3 murrah buffalo bulls (3 ejaculates from each ... | 2017 | 28869110 |
| selection of suitable internal control genes for accurate normalization of real-time quantitative pcr data of buffalo (bubalus bubalis) blastocysts produced by scnt and ivf. | we evaluated the suitability of 10 candidate internal control genes (icgs), belonging to different functional classes, namely actb, eef1a1, gapdh, hprt1, hmbs, rps15, rps18, rps23, sdha, and ubc for normalizing the real-time quantitative polymerase chain reaction (qpcr) data of blastocyst-stage buffalo embryos produced by hand-made cloning and in vitro fertilization (ivf). total rna was isolated from three pools, each of cloned and ivf blastocysts (n = 50/pool) for cdna synthesis. two different ... | 2017 | 28880574 |
| determination of naturally occurring estrogenic hormones in cow's and river buffalo's meat by hplc-fld method. | this study was performed to measure and compare the levels of steroid hormones [estrone (e1), 17β-estradiol (e2), and estriol (e3)] and their conjugated metabolites in cow's and river buffalo's meat in two distinct follicular and luteal phases. moreover, the possible effect of a heating process on steroid hormone concentration was also investigated. the collected meat (biceps femoris muscle) samples were subjected to liquid extraction, enzymatical deconjugation, and c18 solid-phase extraction. e ... | 2016 | 28911549 |
| withdrawn: differential involvement of l- and t-type ca2+ channels, store-operated calcium channel (trpc) and rho-kinase signaling pathway(s) in pgf2α-induced contractions in myometrium of non-pregnant and pregnant buffaloes (bubalus bubalis). | this article has been withdrawn at the request of the author(s) and/or editor. the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at https://www.elsevier.com/about/our-business/policies/article-withdrawal. | 2017 | 28916261 |
| detomidine and butorphanol for standing sedation in a range of zoo-kept ungulate species. | general anesthesia poses risks for larger zoo species, like cardiorespiratory depression, myopathy, and hyperthermia. in ruminants, ruminal bloat and regurgitation of rumen contents with potential aspiration pneumonia are added risks. thus, the use of sedation to perform minor procedures is justified in zoo animals. a combination of detomidine and butorphanol has been routinely used in domestic animals. this drug combination, administered by remote intramuscular injection, can also be applied fo ... | 2017 | 28920785 |
| genetic perspective of milk yield persistency in the first three lactations of iranian buffaloes (bubalus bubalis). | the objectives of this study were to estimate the genetic parameters and genetic trends for different measures of persistency (pm) for milk yield in iranian buffaloes using a random regression test day model. test day records of the first three lactations of buffaloes within 715 herds comprising 43 014, 38 941 and 33 937 records, respectively, were obtained from the animal breeding center of iran during 1992 to 2012. legendre polynomial functions were chosen to fit the lactation curves in the fr ... | 2017 | 28929983 |