Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| a salmonella typhi ompc fusion protein expressing the cd154 trp140-ser149 amino acid strand binds cd40 and activates a lymphoma b-cell line. | cd154 is a type ii glycoprotein member of the tumour necrosis factor (tnf) ligand family, which is expressed mainly on the surface of activated t lymphocytes. the interaction with its receptor cd40, plays a central role in the control of several functions of the immune system. structural models based on the homology of cd154 with tnf and lymphotoxin indicate that binding to cd40 involves three regions surrounding amino acids k143, r203 and q220, and that strands w140-s149 and s198-a210 are criti ... | 2003 | 14511234 |
| characterization of salmonella enterica serotype typhimurium in the czech republic: phage types, antimicrobial and plasmid profiles. | in this study a collection of 547 s. typhimurium strains isolated in the years 2000 and 2001 both of the human and non-human origin were analysed. 21 different phage types were detected, the most frequent one was dt104 (46%) followed by dt141 (28%) and dt68 (3%). resistance to one or more antimicrobial agents was found mainly in dt104 (77.4%). s. typhimurium isolates resistant to 5 and more antimicrobial agents were found in three phagetypes dt104 (57%), dt120 and dt155. plasmid profiling of dt1 ... | 2003 | 14514170 |
| sseg, a virulence protein that targets salmonella to the golgi network. | intracellular replication of the bacterial pathogen salmonella enterica occurs in membrane-bound compartments called salmonella-containing vacuoles (scvs). maturation of the scv has been shown to occur by selective interactions with the endocytic pathway. we show here that after invasion of epithelial cells and migration to a perinuclear location, the majority of scvs become surrounded by membranes of the golgi network. this process is dependent on the salmonella pathogenicity island 2 type iii ... | 2003 | 14517239 |
| salmonella bloodstream infections: report from the sentry antimicrobial surveillance program (1997-2001). | salmonella spp. are significant bloodstream pathogens and are routinely monitored for antimicrobial resistance by the sentry antimicrobial surveillance program. six hundred and one bloodstream infection (bsi) isolates of salmonella spp., collected over a 5-year period (1997-2001) were tested for their susceptibility against 20 antimicrobial agents, comparing year and geographical region. salmonella enterica serotype typhi was the most frequently identified 'species' (43% of identified strains), ... | 2003 | 14522103 |
| activation of hila expression at low ph requires the signal sensor cpxa, but not the cognate response regulator cpxr, in salmonella enterica serovar typhimurium. | a two-component regulatory system, cpxr-cpxa, plays an important role in the ph-dependent regulation of virf, a global activator for virulence determinants including invasion genes, in shigella sonnei. the authors examined whether the cpxr-cpxa homologues have some function in the expression of salmonella enterica serovar typhimurium invasion genes via the regulation of hila, an activator for these genes. in a salmonella cpxa mutant, the hila expression level was reduced to less than 10 % of tha ... | 2003 | 14523114 |
| regulation of expression of the 2-deoxy-d-ribose utilization regulon, deoqkpx, from salmonella enterica serovar typhimurium. | salmonella enterica, in contrast to escherichia coli k12, can use 2-deoxy-d-ribose as the sole carbon source. the genetic determinants for this capacity in s. enterica serovar typhimurium include four genes, of which three, deok, deop, and deox, constitute an operon. the fourth, deoq, is transcribed in the opposite direction. the deok gene encodes deoxyribokinase. in silico analyses indicated that deop encodes a permease and deoq encodes a regulatory protein of the deor family. the deox gene pro ... | 2003 | 14526015 |
| [phenotypic and genotypic methods for epidemiological typing of veterinary important bacterial pathogens of the genera staphylococcus, salmonella, and pasteurella]. | molecular typing methods are capable of providing detailed strain characteristics which are commonly far beyond the capacities of phenotypic typing methods. such molecular-based characteristics have proved to be very helpful in epidemiological studies of bacterial pathogens. the primary criteria that all typing methods should fulfill include (1) the typeability of the strains in question, (2) the reproducibility of the results, and (3) a high discriminatory power. in general, molecular typing me ... | 2003 | 14526470 |
| molecular analysis of the rfb o antigen gene cluster of salmonella enterica serogroup o:6,14 and development of a serogroup-specific pcr assay. | the kauffmann-white scheme for serotyping salmonella recognizes 46 somatic (o) antigen groups, which together with detection of the flagellar (h) antigens form the basis for serotype identification. although serotyping has become an invaluable typing method for epidemiological investigations of salmonella, it does have some practical limitations. we have been characterizing the genes required for o and h antigen biosynthesis with the goal of developing a dna-based system for the determination of ... | 2003 | 14532067 |
| a model to estimate the optimal sample size for microbiological surveys. | estimating optimal sample size for microbiological surveys is a challenge for laboratory managers. when insufficient sampling is conducted, biased inferences are likely; however, when excessive sampling is conducted valuable laboratory resources are wasted. this report presents a statistical model for the estimation of the sample size appropriate for the accurate identification of the bacterial subtypes of interest in a specimen. this applied model for microbiology laboratory use is based on a b ... | 2003 | 14532078 |
| association between handling of pet treats and infection with salmonella enterica serotype newport expressing the ampc beta-lactamase, cmy-2. | resistance to the extended-spectrum cephalosporins can occur in salmonella species via the production of extended-spectrum and ampc beta-lactamases. we describe human infections with salmonella enterica serotype newport phage type 14 strains resistant to ceftazidime (caz) and cefoxitin (fox) related to the handling of pet treats containing dried beef. these strains were isolated from five patients in calgary, alberta, canada, during 2002 and were compared to a strain cultured from a commercial p ... | 2003 | 14532185 |
| subtyping of salmonella enterica serovar typhimurium outbreak strains isolated from humans and animals in iceland. | a total of 75 salmonella enterica serovar typhimurium strains of various (mainly human and animal) origins were typed by pulsed-field gel electrophoresis (pfge) and phage typing. these strains were collected during an outbreak in iceland in 1999 and 2000. the typing revealed that 84% of the strains belonged to the same pfge and phage type (pt), namely, pfge type 1aa and pt 1. | 2003 | 14532235 |
| isolation of salmonella enterica from slaughtered pigs. | 2003 | 14535410 | |
| preparation and characterization of polyclonal and monoclonal antibodies against lawsonia intracellularis. | proliferative enteropathy is an intestinal infectious disease caused by the obligate intracellular bacterium lawsonia intracellularis. immunohistochemistry staining has superior sensitivity over hematoxylin and eosin and silver staining for detecting l. intracellularis in histological sections. a l. intracellularis-specific monoclonal antibody (mab) produced in the uk (ig4 mab) has been described in the literature. however, no monoclonal or polyclonal antibodies are commercially available. there ... | 2003 | 14535543 |
| an outbreak of salmonella enterica serovar typhimurium (s. typhimurium) phage type 135a in the greater murray. | 2003 | 14552311 | |
| transcriptional analysis of the rpoe gene encoding extracytoplasmic stress response sigma factor sigmae in salmonella enterica serovar typhimurium. | the rpoe gene of salmonella enterica serovar typhimurium (s. typhimurium), which encodes the extracytoplasmic stress response sigma factor sigmae, is critically important for the virulence of s. typhimurium. we analysed expression of rpoe by wild-type and mutant bacteria grown in different conditions by s1-nuclease mapping using rna, and using in vivo reporter gene fusions. three promoters, rpoep1, rpoep2 and rpoep3, were located upstream of the s. typhimurium rpoe gene. the promoters were diffe ... | 2003 | 14553927 |
| acidic ph is required for the functional assembly of the type iii secretion system encoded by salmonella pathogenicity island 2. | salmonella enterica employs two type iii secretion systems (t3ss) for interactions with host cells during pathogenesis. the t3ss encoded by salmonella pathogenicity island 2 (spi2) is required for the intracellular replication of salmonella and the survival inside phagocytes. during growth in vitro, acidic ph is a signal that promotes secretion of proteins by this t3ss. we analyzed protein levels and subcellular localization of various t3ss subunits under in vitro conditions at acidic or neutral ... | 2003 | 14553934 |
| proteome analysis of salmonella enterica serovar typhimurium fis mutant. | salmonella enterica serovar typhimurium is an enteric pathogen and a principal cause of gastroenteritis in humans. the factor-for-inversion stimulation protein (fis) is known to play a pivotal role in the expression of salmonella pathogenicity island (spi)-1 genes in addition to various cellular processes such as recombination, replication, and transcription. in order to understand fis function in pathogenicity of salmonella, we performed two-dimensional gel electrophoresis and identified protei ... | 2003 | 14553938 |
| searching for keys under the devil's lamppost. | 2003 | 14557027 | |
| molecular characterization of the mg2+-responsive phop-phoq regulon in salmonella enterica. | the phop/phoq two-component system controls the extracellular magnesium deprivation response in salmonella enterica. in addition, several virulence-associated genes that are mainly required for intramacrophage survival during the infection process are under the control of its transcriptional regulation. despite shared mg(2+) modulation of the expression of the phop-activated genes, no consensus sequence common to all of them could be detected in their promoter regions. we have investigated the t ... | 2003 | 14563863 |
| a new heat shock gene, agsa, which encodes a small chaperone involved in suppressing protein aggregation in salmonella enterica serovar typhimurium. | we discovered a novel small heat shock protein (shsp) named agsa (aggregation-suppressing protein) in the thermally aggregated fraction from a salmonella enterica serovar typhimurium dnak-null strain. the -10 and -35 regions upstream of the transcriptional start site of the agsa gene are characteristic of sigma(32)- and sigma(72)-dependent promoters. agsa was strongly induced by high temperatures. the similarity between agsa and the other two shsps of salmonella serovar typhimurium, ibpa and ibp ... | 2003 | 14563868 |
| bacteriophage st64b, a genetic mosaic of genes from diverse sources isolated from salmonella enterica serovar typhimurium dt 64. | the complete sequence of the double-stranded dna (dsdna) genome of the salmonella enterica serovar typhimurium st64b bacteriophage was determined. the 40,149-bp genomic sequence of st64b has an overall g+c content of 51.3% and is distinct from that of p22. the genome architecture is similar to that of the lambdoid phages, particularly that of coliphage lambda. most of the putative tail genes showed sequence similarity to tail genes of mu, a nonlambdoid phage. in addition, it is likely that these ... | 2003 | 14563886 |
| recovery of surface bacteria from and surface sanitization of cantaloupes. | practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. cantaloupes (cucumis melo l. var. reticulates) were immersed in an inoculum containing salmonella enterica serovar poona or pantoea agglomerans at ca. 10(4) to 10(5) cfu/ml. an efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. the method consisted of wa ... | 2003 | 14572217 |
| antibacterial activities of phenolic benzaldehydes and benzoic acids against campylobacter jejuni, escherichia coli, listeria monocytogenes, and salmonella enterica. | we evaluated the bactericidal activities of 35 benzaldehydes, 34 benzoic acids, and 1 benzoic acid methyl ester against campylobacter jejuni, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica when these compounds were substituted on the benzene ring with 0, 1, 2, or 3 hydroxy (oh) and/or methoxy (och3) groups in a ph 7.0 buffer. dose-response plots were used to determine the percentage of the sample that induced a 50% decrease in cfu after 60 min (ba50). of the 70 compoun ... | 2003 | 14572218 |
| salmonella enterica serovar typhimurium expressing mutant lipid a with decreased endotoxicity causes maturation of murine dendritic cells. | a major salmonella component involved in cellular activation is the lipopolysaccharide (lps) molecule which can act as a dendritic cell (dc) stimulator. the structure of the lipid a domain of the lps molecule dictates its immunostimulatory capacity with various cell types. in this study, the role of lipid a as an integral component of salmonella in stimulating murine dcs was studied by using a salmonella enterica serovar typhimurium lpxm mutant with defective lipid a. this study revealed that a ... | 2003 | 14573628 |
| autodisplay: efficacious surface exposure of antigenic urea fragments from helicobacter pylori in salmonella vaccine strains. | live attenuated salmonella strains expressing antigens of pathogens are promising oral vaccine candidates. there is growing evidence that the topology of expression of the foreign antigens can have a dramatic impact on the immunogenicity. we examined the potential of the aida-i (escherichia coli adhesin involved in diffuse adherence) autotransporter domain to display antigenic fragments of the urease a subunit of helicobacter pylori for the induction of a protective immune response. in the murin ... | 2003 | 14573651 |
| type 1 fimbriae of salmonella enterica serovar typhimurium bind to enterocytes and contribute to colonization of swine in vivo. | salmonella enterica serovar typhimurium strain 798 is a clinical isolate from a pig and is known to be able to cause persistent, asymptomatic infections. this strain also is known to exist in two phenotypes (adhesive and nonadhesive to enterocytes) and can switch between the two phenotypes at a rate consistent with phase variation. cells in the adhesive phenotype are more readily phagocytosed by leukocytes than nonadhesive cells. once in a leukocyte, adhesive-phase cells survive while nonadhesiv ... | 2003 | 14573666 |
| capg(-/-) mice have specific host defense defects that render them more susceptible than capg(+/+) mice to listeria monocytogenes infection but not to salmonella enterica serovar typhimurium infection. | loss of the actin filament capping protein capg has no apparent effect on the phenotype of mice maintained under sterile conditions; however, bone marrow-derived macrophages from capg(-/-) mice exhibited distinct motility defects. we examined the ability of capg(-/-) mice to clear two intracellular bacteria, listeria monocytogenes and salmonella enterica serovar typhimurium. the 50% lethal dose of listeria was 10-fold lower for capg(-/-) mice than for capg(+/+) mice (6 x 10(3) cfu for capg(-/-) ... | 2003 | 14573680 |
| commensal bacteria increase invasion of intestinal epithelium by salmonella enterica serovar typhi. | the intestinal microflora consists of a heterogeneous population of microorganisms and has many effects on the health status of its human host. here, it is shown that the products of certain strains of bacteria normally present in the intestinal microflora are able to trigger redistribution of the cystic fibrosis transmembrane conductance regulator (cftr) protein in epithelial cells. cftr is used by salmonella enterica serovar typhi as a receptor on epithelial cells which mediate the translocati ... | 2003 | 14573683 |
| toll-like receptor expression in c3h/hen and c3h/hej mice during salmonella enterica serovar typhimurium infection. | here, we have investigated the mrna expression of toll-like receptor 2 (tlr-2), tlr-4, and md-2 in spleens and livers of c3h/hen mice (carrying wild-type tlr-4) and c3h/hej mice (carrying mutated tlr-4) in response to salmonella infection. during salmonella infections, tlr-4 is activated, leading to increased tlr-2 and decreased tlr-4 expression. | 2003 | 14573691 |
| secretion of the orgc gene product by salmonella enterica serovar typhimurium. | hila activates the transcription of genes on salmonella pathogenicity island 1 (spi1), which encodes a type iii secretion system (ttss). previous studies showed that transposon insertions in orgc, a gene located on spi1, increase hila expression. we characterize the orgc gene product and show that it is secreted via the spi1 ttss. we propose a model whereby orgc functions as a secreted repressor of the spi1 virulence genes. | 2003 | 14573697 |
| mutations in topoisomerase genes of fluoroquinolone-resistant salmonellae in hong kong. | a total of 88 salmonella isolates (72 clinical isolates for which the ciprofloxacin mic was >0.06 microg/ml, 15 isolates for which the ciprofloxacin mic was < or =0.06 microg/ml, and salmonella enterica serotype typhimurium atcc 13311) were studied for the presence of genetic alterations in four quinolone resistance genes, gyra, gyrb, parc, and pare, by multiplex pcr amplimer conformation analysis. the genetic alterations were confirmed by direct nucleotide sequencing. a considerable number of s ... | 2003 | 14576119 |
| multiple-drug resistance in d-tartrate-positive salmonella enterica serovar paratyphi b isolates from poultry is mediated by class 2 integrons inserted into the bacterial chromosome. | the presence of integrons in 85 multiresistant german isolates of the predominating salmonella enterica subsp. enterica serovar paratyphi b dt(+) clone was investigated. all isolates possessed a chromosomally located tn7-like class 2 integron carrying the same dfra1-sat1-aada1 array of gene cassettes. only four isolates (4.7%) revealed an additional class 1 integron with two strains each containing the aada1 or dfra1-aada1 gene cassettes. | 2003 | 14576136 |
| salmonella enterica serovar dublin strains which are vi antigen-positive use type ivb pili for bacterial self-association and human intestinal cell entry. | some strains of salmonella enterica serovar dublin are vi antigen-positive. s. enterica serovar typhi uses type ivb pili, encoded adjacent to the viab locus required for vi antigen synthesis, to facilitate both eukaryotic cell attachment and bacterial self-association under conditions that favour dna supercoiling. these pilus-mediated events may be important in typhoid fever pathogenesis. a survey of 17 isolates of s. enterica serovar dublin showed that all strains which carried the viab region ... | 2003 | 14580391 |
| multiplex polymerase chain reaction assay for selective detection of salmonella enterica serovar typhimurium. | a multiplex polymerase chain reaction (pcr) assay was developed for the identification of salmonella enterica serovar typhimurium. three sets of primers were designed for detecting o4, h:i, and h:1,2 antigen genes from the antigen-specific genes rfbj, flic, and fljb, respectively. these were evaluated in a multiplex pcr assay by using dnas from s. enterica serovar typhimurium, 15 other salmonella serovars, and 8 non-salmonella enteric pathogens. multiplex pcr proved to be capable of identifying ... | 2003 | 14583637 |
| combination effect of ciprofloxacin and gentamicin against clinical isolates of salmonella enterica serovar typhi with reduced susceptibility to ciprofloxacin. | the present study evaluated the in vitro efficacy of ciprofloxacin (cpfx) in combination with gentamicin (gm) using agar dilution checkerboard method against six blood culture isolates of salmonella enterica serovar typhi with cpfx minimum inhibitory concentration (mic) values of 0.75 - 1.25 microg/ml and gm mic values of 0.75 - 2 microg/ml. when used in combination, the fractional inhibitory concentration (fic) values of cpfx and gm for the isolates ranged from 0.008 - 0.032 microg/ml and 0.1 - ... | 2003 | 14583638 |
| observations on the distribution and persistence of salmonella enterica serovar enteritidis phage type 29 on a cage layer farm before and after the use of competitive exclusion treatment. | 1. a continuously occupied cage layer house, which had been linked with a human outbreak of salmonella enteritidis pt29, was investigated to assess the distribution of contamination and the options for control. 2. the presence and persistence of salmonella before and after application of competitive exclusion (ce) treatment was investigated by culturing samples from faeces, the environment, spent hens and eggs, and use of an elisa to detect egg yolk antibodies. 3. a high prevalence of salmonella ... | 2003 | 14584845 |
| delivery of a heterologous antigen by a registered salmonella vaccine (stm1). | stm1 is an aro a(-) attenuated mutant of salmonella enterica serovar typhimurium, and is a well-characterised vaccine strain available to the livestock industry for the prevention of salmonellosis in chickens. this strain has potential for heterologous antigen delivery, and here we show that the strain can be used to deliver a model antigen, ovalbumin, to immune cells in vitro and in vivo. two plasmid constructs expressing the ovalbumin gene were utilised, one of which uses a prokaryotic promote ... | 2003 | 14592711 |
| the molecular characterisation of the extended spectrum beta-lactamase (esbl) producing strain of salmonella enterica serovar mbandaka isolated in poland. | the molecular attributes of the first esbl producing isolate 267/99 of salmonella enterica serovar mbandaka isolated in 1999 in poland were characterised. tested strain produced ctx-m-3 and tem-1 beta-lactamases, encoded by genes localised on the about 80 kb self-transferable plasmid, harbouring the 2 kb class 1 integron carrying aada2 gene. | 2003 | 14594405 |
| negative osmoregulation of the salmonella omps1 porin gene independently of ompr in an hns background. | the omps1 gene encodes a quiescent porin in salmonella enterica serovars typhi and typhimurium. by using random mariner transposon mutagenesis, mutations that caused derepression of omps1 expression were isolated, one in s. enterica serovar typhi and two in s. enterica serovar typhimurium. all of them mapped in the hns gene in the region coding for the carboxy terminus of the h-ns nucleoid protein. the derepressed omps1 expression was subject to negative regulation at high osmolarity, both in th ... | 2003 | 14594821 |
| soxrs-regulated expression and genetic analysis of the yggx gene of escherichia coli. | genomic studies with bacteria have identified redox-responsive genes without known roles in counteracting oxidative damage. previous transcriptional profiling showed that expression of one such gene, yggx, was activated by superoxide stress in escherichia coli. here we show that this activation could be mimicked by artificial expression of the regulatory protein soxs. northern analysis confirmed the transcriptional activation of yggx by oxidative stress or soxs expression but not in response to ... | 2003 | 14594836 |
| a mutation in the essential gene gmk (encoding guanlyate kinase) generates a requirement for adenine at low temperature in salmonella enterica. | in salmonella enterica serovar typhimurium, gmk encodes guanylate kinase, an essential enzyme involved in the synthesis and salvage of guanine nucleotides. here we report the isolation of a mutation in gmk that results in a nutritional requirement for adenine at low temperature. comparisons of kinetic parameters from the wild-type and mutant gmk enzymes revealed that the mutant enzyme had a more than 20-fold-higher km for atp than the wild-type enzyme. the growth dependence of the mutant on temp ... | 2003 | 14594851 |
| cysg structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis. | sulfur metabolism depends on the iron-containing porphinoid siroheme. in salmonella enterica, the s-adenosyl-l-methionine (sam)-dependent bismethyltransferase, dehydrogenase and ferrochelatase, cysg, synthesizes siroheme from uroporphyrinogen iii (uro'gen iii). the reactions mediated by cysg encompass two branchpoint intermediates in tetrapyrrole biosynthesis, diverting flux first from protoporphyrin ix biosynthesis and then from cobalamin (vitamin b(12)) biosynthesis. we determined the first st ... | 2003 | 14595395 |
| priming by recombinant chicken interleukin-2 induces selective expression of il-8 and il-18 mrna in chicken heterophils during receptor-mediated phagocytosis of opsonized and nonopsonized salmonella enterica serovar enteritidis. | heterophils, the principal avian polymorphonuclear leukocytes (pmns) equivalent to the mammalian neutrophil, function as professional phagocytes against bacterial infections, mediate acute inflammation, and respond to cytokine stimulation to aid in regulation of innate host defenses. interleukin-2 (il-2) has been found to exercise an array of biological effects on other cell types besides t lymphocytes, including nk cells, b cells, monocytes, and neutrophils. in the present experiments, using re ... | 2003 | 14597163 |
| tetra-amino-acid tandem repeats are involved in hsds complementation in type ic restriction-modification systems. | all known type i restriction and modification (r-m) systems of escherichia coli and salmonella enterica belong to one of four discrete families: type ia, ib, ic or id. the classification of type i systems from a wide range of other genera is mainly based on complementation and molecular evidence derived from the comparison of the amino acid similarity of the corresponding subunits. this affiliation was seldom based on the strictest requirement for membership of a family, which depends on related ... | 2003 | 14600243 |
| trends in antimicrobial drug resistance in salmonella enterica serotypes typhi and paratyphi a isolated in europe, 1999-2001. | results of antimicrobial sensitivity tests for strains of salmonella enterica serotypes typhi and paratyphi a isolated from patients in ten european countries between 1999 and 2001 have been transferred electronically to the enter-net surveillance hub. for typhi between 22 and 29% of isolates were multiresistant (to four drugs or more) with decreased susceptibility to ciprofloxacin (mic 0.25-1.0 mg/l) increasing from 20% in 1999 to 26% in 2001. nineteen of 169 (11%) strains with decreased ciprof ... | 2003 | 14602366 |
| in vitro fluoroquinolone-resistant mutants of salmonella enterica serotype enteritidis: analysis of mechanisms involved in resistance. | this study analysed the mechanisms involved in the acquisition of resistance to quinolones in mutants obtained in vitro of salmonella enterica serotype enteritidis. two nalidixic acid-resistant (minimal inhibitory concentrations, mic>256 mg/l), ciprofloxacin-susceptible (mic 0.5 mg/l) clinical isolates of salmonella enteritidis with a mutation at amino acid codon ser-83 of the gyra gene were grown on plates containing increasing concentrations of ciprofloxacin. the increase in mic to ciprofloxac ... | 2003 | 14602375 |
| induction and resuscitation of viable but nonculturable salmonella enterica serovar typhimurium dt104. | salmonella enterica serovar typhimurium dt104 11601 was tested for its ability to maintain viability in minimal, chemically defined solutions. periodic monitoring of growth and survival in microcosms of different ion concentrations, maintained at various temperatures, showed a gradual decline in culturable organisms ( approximately 235 days) at 5 degrees c. organisms maintained at a higher temperature (21 degrees c) showed continuous, equivalent cfu per milliliter ( approximately 10(6)) up to 40 ... | 2003 | 14602627 |
| the tubex typhoid test based on particle-inhibition immunoassay detects igm but not igg anti-o9 antibodies. | a serological test kit (tubex, idl biotech, sweden) developed recently for the diagnosis of typhoid fever detects antibodies to the salmonella enterica serovar typhi lipopolysaccharide (lps) o9 antigen. the antibodies are detected by their ability to inhibit the interaction between two types of reagent particles: (a). indicator latex microspheres sensitized with an anti-o9 monoclonal antibody, and (b). magnetic microspheres sensitized with s. typhi lps. following rapid mixing of the serum with t ... | 2003 | 14604543 |
| a regional outbreak of s. enteritidis phage type 5, traced back to the flocks of an egg producer, austria. | in the spring and summer of 2002, the nationale referenzzentrale für salmonellen (national reference centre for salmonella - nrcs) in austria noticed a cluster of human salmonella enterica subsp. enterica ser. enteritidis phage type 5 (s. enteritidis pt5) infections in two neighbouring districts of austria. another small outbreak of s. enteritidis pt5 infections that occurred in the same region in 1999 had been traced back to the flocks of a local egg producer (approximately 6 000 hens). attenti ... | 2003 | 14605374 |
| a simple method for semi-preparative-scale production and recovery of enterocin as-48 derived from enterococcus faecalis subsp. liquefaciens a-48-32. | production of enterocin as-48 by enterococcus faecalis a-48-32 was compared between standard and high-cell density batch fermentations. in high-cell density cultures, bacteriocin production was 2.47-fold higher, provided that the ph was controlled during the fermentation. a two-step procedure for recovery of milligram quantities of purified bacteriocin was developed, based on adsorption of the bacteriocin on carboxymethyl sephadex cm-25 followed by reversed-phase chromatography on a semi-prepara ... | 2003 | 14607403 |
| mimotopes of the vi antigen of salmonella enterica serovar typhi identified from phage display peptide library. | the capsular polysaccharide vi antigen (vicps) is an essential virulence factor and also a protective antigen of salmonella enterica serovar typhi. a random 12-mer phage-displayed peptide library was used to identify mimotopes (epitope analogues) of this antigen by panning against a vicps-specific monoclonal antibody (mab) atvi. approximately 75% of the phage clones selected in the fourth round carried the peptide sequence tshhdshglhrv, and the rest of the clones harbored enhspvniahkl and other ... | 2003 | 14607870 |
| a prospective study of phage types & biotypes of salmonella enterica serotype typhi isolated from hospitalized children in kolkata, india. | kolkata and its suburbs in eastern india faced an epidemic of typhoid fever in 1990. a prospective, hospital and laboratory based study over a period of 12 yr (1990-2001), on the phage typing and biotyping pattern of salmonella enterica serotype typhi was carried out, to see if there has been a change. | 2003 | 14609047 |
| cephalosporin-resistant escherichia coli among summer camp attendees with salmonellosis. | 2003 | 14609463 | |
| reference map of soluble proteins from salmonella enterica serovar enteritidis by two-dimensional electrophoresis. | protein identification by peptide mass fingerprinting using matrix-assisted laser desorption ionization time of fight (maldi-tof) mass spectrometry (ms) can analyze unambiguously identity of the spots from a 2-dimensional electrophoresis (2-de) gel. this study developed a technique for 2-de of salmonella enterica serovar enteritidis (s. enteritidis) by improving the dissolution conditions by 2-de using a ph 4 - 7 immobilized ph gradient (ipg) strip. this report examines the protein components fr ... | 2003 | 14610367 |
| pancreatic involvement in salmonella infection. | salmonella has been identified as a causative agent of acute pancreatitis. | 2003 | 14614200 |
| swarming populations of salmonella represent a unique physiological state coupled to multiple mechanisms of antibiotic resistance. | salmonella enterica serovar typhimurium is capable of swarming over semi-solid surfaces. although its swarming behavior shares many readily observable similarities with other swarming bacteria, the phenomenon remains somewhat of an enigma in this bacterium since some attributes skew away from the better characterized systems. swarming is quite distinct from the classic swimming motility, as there is a prerequisite for cells to first undergo a morphological transformation into swarmer cells. in s ... | 2003 | 14615815 |
| a european outbreak of salmonella enterica serotype typhimurium definitive phage type 204b in 2000. | to describe the clinical, epidemiologic and microbiological features of a large outbreak of infection with a multiresistant salmonella enterica serotype typhimurium definitive type dt204b infection involving at least 392 people in five european countries. | 2003 | 14616705 |
| antimicrobial resistance in salmonella enterica var. enteritidis human isolates in bosnia and herzegovina. | 2003 | 14616739 | |
| role of the salmonella pathogenicity island 1 (spi-1) protein invb in type iii secretion of sope and sope2, two salmonella effector proteins encoded outside of spi-1. | salmonella enterica subspecies 1 serovar typhimurium encodes a type iii secretion system (ttss) within salmonella pathogenicity island 1 (spi-1). this ttss injects effector proteins into host cells to trigger invasion and inflammatory responses. effector proteins are recognized by the ttss via signals encoded in their n termini. specific chaperones can be involved in this process. the chaperones invb, sica, and sicp are encoded in spi-1 and are required for transport of spi-1-encoded effectors. ... | 2003 | 14617659 |
| [antimicrobial action of ammonium salts of fused heterocycles containing ortho-nitrogen]. | by alkylation of hexamethylenetetramine with halogenated derivatives of ketones, ethers, esters or amides of acids, alkyl- and aralkyl halides the corresponding n-monoalkylated compounds of hexamethylenetetramine were obtained. the quaternization of pyridine nitrogen in 5,6-benzoquinoline, 8-hydroxyquinoline, quinoline, 1,10-phenanthroline molecules with alkyl- or aralkylhalides was carried out. the susceptibility of gram-positive (streptococcus agalactiae and staphylococcus aureus) and gram-neg ... | 2003 | 14617863 |
| epidemiology of salmonella enterica serovars enteritidis and typhimurium in animals and people in scotland between 1990 and 2001. | two serovars of salmonella which are currently of particular importance in both human and animal infections are salmonella enterica serovars enteritidis phage type 4 (pt4) and typhimurium definitive type 104 (dt104). this paper describes the trends in the relationships between the levels of infection of people and a range of farm animal species with these two serovars and explores some of the reasons behind them. in 1996, there was a peak of 520 reports of s typhimurium dt104 infection in people ... | 2003 | 14620550 |
| bacterial infection induces expression of functional mhc class ii molecules in murine and human osteoblasts. | a growing body of evidence has shown that bacterially challenged osteoblasts can play a significant role in the initiation of inflammatory immune responses at sites of bone disease. we have recently demonstrated the surprising ability of osteoblasts exposed to bacteria to express cd40, a molecule that plays a critical costimulatory role in the activation of t lymphocytes. in the present study, we have extended our investigations into the ability of osteoblasts to interact with cd4+ t lymphocytes ... | 2003 | 14623057 |
| reduction of salmonella enterica on alfalfa seeds with acidic electrolyzed oxidizing water and enhanced uptake of acidic electrolyzed oxidizing water into seeds by gas exchange. | alfalfa sprouts have been implicated in several salmonellosis outbreaks in recent years. the disinfectant effects of acidic electrolyzed oxidizing (eo) water against salmonella enterica both in an aqueous system and on artificially contaminated alfalfa seeds were determined. the optimum ratio of seeds to eo water was determined in order to maximize the antimicrobial effect of eo water. seeds were combined with eo water at ratios (wt/vol) of 1:4, 1:10, 1:20, 1:40, and 1:100, and the characteristi ... | 2003 | 14627277 |
| nitric oxide produced by murine dendritic cells is cytotoxic for intracellular salmonella enterica sv. typhimurium. | the pathogenicity of salmonella enterica serovar typhimurium has traditionally been correlated with its ability to survive and grow in macrophages. macrophage-derived production of nitric oxide (no) has been implicated as a major innate defence, restricting bacterial proliferation both in macrophage cultures and in mice. in the present study, we show that the ability of primary murine dendritic cells (dcs) to ingest salmonella is low, but greatly enhanced by serum complement. ingestion of bacter ... | 2003 | 14629621 |
| the effect of chlortetracycline treatment and its subsequent withdrawal on multi-resistant salmonella enterica serovar typhimurium dt104 and commensal escherichia coli in the pig. | to investigate the effect of a therapeutic and sub-therapeutic chlortetracycline treatment on tetracycline-resistant salmonella enterica serovar typhimurium dt104 and on the commensal escherichia coli in pig. | 2003 | 14632995 |
| nucleic acid sequence based amplification for the rapid and sensitive detection of salmonella enterica from foods. | the purpose of this study was to apply nucleic acid sequence-based amplification (nasba) for the detection of salmonella enterica serovar enteritidis (s. enteritidis) in representative foods. | 2003 | 14633009 |
| dna fingerprinting of human isolates of salmonella enterica serotype paratyphi b in malaysia. | dna fingerprinting of salmonella enterica serotype paratyphi b isolated in malaysia during 1982-83, 1992 and 1996-2002 was carried out by pulsed-field gel electrophoresis (pfge), antimicrobial susceptibility tests and d-tartrate utilization tests to assess the extent of genetic diversity of these isolates in malaysia. | 2003 | 14633043 |
| activity of natural antimicrobial compounds against escherichia coli and salmonella enterica serovar typhimurium. | the objective of this study was to evaluate the inhibitory activity of several natural organic compounds alone or in combination with nisin against escherichia coli and salmonella typhimurium. | 2003 | 14633097 |
| assessment of the presence of salmonella spp. in egyptian dairy products using various detection media. | to make a preliminary assessment of the incidence of salmonella in egyptian dairy products, and to investigate the effectiveness of various protocols for the detection of the pathogen in these products. | 2003 | 14633112 |
| temporal regulation of salmonella virulence effector function by proteasome-dependent protein degradation. | salmonella enterica invasion of host cells requires the reversible activation of the rho-family gtpases cdc42 and rac1 by the bacterially encoded gef sope and the gap sptp, which exert their function at different times during infection and are delivered into host cells by a type iii secretion system. we found that sope and sptp are delivered in equivalent amounts early during infection. however, sope is rapidly degraded through a proteosome-mediated pathway, while sptp exhibits much slower degra ... | 2003 | 14636560 |
| reduced amounts of lps affect both stress tolerance and virulence of salmonella enterica serovar dublin. | signature-tagged mutagenesis (stm) is a widely used technique for identification of virulence genes in bacterial pathogens. while this approach often generates a large number of mutants with a potential reduction in virulence a major task is subsequently to determine the mechanism by which the mutations influence virulence. presently, we have characterised a salmonella enterica serovar dublin stm mutant that, in addition to having reduced virulence, was also impaired when growing under various s ... | 2003 | 14638428 |
| fusidic acid-resistant mutants of salmonella enterica serovar typhimurium with low fitness in vivo are defective in rpos induction. | mutants of salmonella enterica serovar typhimurium resistant to fusidic acid (fusr) have mutations in fusa, the gene encoding translation elongation factor g (ef-g). most fusr mutants have reduced fitness in vitro and in vivo, in part explained by mutant ef-g slowing the rate of protein synthesis and growth. however, some fusr mutants with normal rates of protein synthesis still suffer from reduced fitness in vivo. as shown here, fusr mutants could be similarly ranked in their relative fitness i ... | 2003 | 14638476 |
| genehunter, a transposon tool for identification and isolation of cryptic antibiotic resistance genes. | genehunter is a transposon tool designed for the experimental activation and identification of silent antibiotic resistance genes. the method permits the identification of novel resistance genes that lack previously identified homologues. using salmonella enterica serovar typhimurium strain lt2 as a test organism for the in vivo version of the genehunter method, we were able to activate, clone, and identify two cryptic antibiotic resistance genes, the aminoglycoside acetyltransferase aac(6')-iaa ... | 2003 | 14638492 |
| association of salmonella enterica serovar enteritidis yafd with resistance to chicken egg albumen. | salmonella enterica serovar enteritidis is a major cause of food-borne diseases in industrialized countries. the incidence of s. enterica serovar enteritidis infections has increased substantially in recent decades, and s. enterica serovar enteritidis is now one of the leading serovars of salmonella in the united states. a unique epidemiological characteristic of s. enterica serovar enteritidis is its association with chicken shell eggs, since approximately 80% of all human gastrointestinal dise ... | 2003 | 14638758 |
| characterization and development of t-cell immune responses in b-cell-deficient (igh-6(-/-)) mice with salmonella enterica serovar typhimurium infection. | infection of mice with salmonella enterica serovar typhimurium induces strong th1 t-cell responses that are central to the control of the infection. in the present study, we examined the role of b cells in the development of th1 t-cell responses to salmonella by using gene-targeted b-cell-deficient mice (igh-6(-/-) mice). the development of th1 t-cell responses in igh-6(-/-) mice was impaired in the early stage of a primary infection. this impairment persisted throughout the course of the diseas ... | 2003 | 14638767 |
| role of receptor proteins for enterobactin and 2,3-dihydroxybenzoylserine in virulence of salmonella enterica. | single, double, and triple mutants of an enterobactin-deficient mutant strain of salmonella enterica serovar typhimurium were constructed that were defective in the expression of the iron-regulated outer membrane proteins (iromps) fepa, iron, and cir, which are proposed to function as catecholate receptors. uptake of naturally occurring and chemically synthesized catecholate molecules by these mutants was assessed in standard growth promotion assays. unique patterns of uptake were identified for ... | 2003 | 14638784 |
| comparative analysis of salmonella enterica serovar typhimurium biofilm formation on gallstones and on glass. | in this study, the roles of global regulators, motility, lipopolysaccharide, and exopolysaccharides were further characterized with respect to biofilm formation on both gallstones and glass surfaces. these studies show the complex nature of biofilms and demonstrate that characteristics observed for each biofilm are unique to the particular culture condition. | 2003 | 14638807 |
| emergence of multidrug-resistant salmonella enterica serotype newport infections resistant to expanded-spectrum cephalosporins in the united states. | we describe a field investigation in new england that identified the emergence and epidemiology of new strains of multidrug-resistant salmonella, newport-mdrampc, and summarize the center for disease control and prevention's surveillance data for these infections. in massachusetts, the prevalence of newport-mdrampc among salmonella serotype newport isolates obtained from humans increased from 0% (0/14) in 1998 to 53% (32/60) in 2001 (p<.001). in a retrospective case-control study, infection with ... | 2003 | 14639542 |
| role of prokaryotic cu,zn superoxide dismutase in pathogenesis. | several bacterial pathogens possess sodc genes that encode periplasmic or membrane-associated cu,zn superoxide dismutases. since professional phagocytes generate large amounts of reactive oxygen species to control the growth of invading micro-organisms, cu,zn superoxide dismutase might protect infectious bacteria from oxy-radical damage and facilitate their survival within the host. this idea has gained support from studies showing that sodc -null mutants of different bacteria are less virulent ... | 2003 | 14641055 |
| intracellular activities of salmonella enterica in murine dendritic cells. | dendritic cells (dc) efficiently phagocytose invading bacteria, but fail to kill intracellular pathogens such as salmonella enterica serovar typhimurium (s. typhimurium). we analysed the intracellular fate of salmonella in murine bone marrow-derived dc (bm-dc). the intracellular proliferation and subcellular localization were investigated for wild-type s. typhimurium and mutants deficient in salmonella pathogenicity island 2 (spi2), a complex virulence factor that is essential for systemic infec ... | 2003 | 14641178 |
| rpos and rpon are involved in the growth-dependent regulation of rfah transcription and o antigen expression in salmonella enterica serovar typhi. | we reported earlier that the production of o antigen lipopolysaccharide (lps) by salmonella enterica serovar typhi (salmonella typhi) increases at the onset of stationary phase and correlates with a growth-regulated expression of the rfah gene under the control of the alternative sigma factor rpon (microbiology 148 (2002) 3789). in this study, we demonstrate that rpos also modulates rfah promoter activity as revealed by the absence of growth-dependent regulation of an rfah-lacz transcriptional f ... | 2004 | 14643636 |
| activation of innate immune responses by il-2-expressing salmonella typhimurium is independent of toll-like receptor 4. | we previously demonstrated that an attenuated strain of salmonella enterica serovar typhimurium, engineered to express il-2 (strain gidil2), is cleared more rapidly than its parental, non-cytokine-expressing, strain (designated brd509) from the reticuloendothelial system of susceptible balb/c mice. this early clearance correlated with the induction of a strong innate immune response within a few hours of administration of gidil2 organisms. in the present study, we wished to assess the contributi ... | 2004 | 14644093 |
| a new pathway for salvaging the coenzyme b12 precursor cobinamide in archaea requires cobinamide-phosphate synthase (cbib) enzyme activity. | the ability of archaea to salvage cobinamide has been under question because archaeal genomes lack orthologs to the bacterial nucleoside triphosphate:5'-deoxycobinamide kinase enzyme (cobu in salmonella enterica). the latter activity is required for cobinamide salvaging in bacteria. this paper reports evidence that archaea salvage cobinamide from the environment by using a pathway different from the one used by bacteria. these studies demanded the functional characterization of two genes whose p ... | 2003 | 14645280 |
| pathways leading from bara/sira to motility and virulence gene expression in salmonella. | the bara and sira genes of salmonella enterica serovar typhimurium encode a two-component sensor kinase and a response regulator, respectively. this system increases the expression of virulence genes and decreases the expression of motility genes. in this study, we examined the pathways by which sira affects these genes. we found that the master regulator of flagellar genes, flhdc, had a positive regulatory effect on the primary regulator of intestinal virulence determinants, hila, but that hila ... | 2003 | 14645287 |
| invb is a type iii secretion-associated chaperone for the salmonella enterica effector protein sope. | sope is a bacteriophage-encoded effector protein of salmonella enterica serovar typhimurium that is translocated into the cytosol of eukaryotic cells by a type iii secretion system (ttss) (w.-d. hardt, h. urlaub, and j. e. galán, proc. natl. acad. sci. usa 95:2574-2579, 1998; m. w. wood, r. rosqvist, p. b. mullan, m. h. edwards, and e. e. galyov, mol. microbiol. 22:327-338, 1996). in this study, we provide evidence that an unlinked gene carried within the salmonella pathogenicity island 1 (spi-1 ... | 2003 | 14645290 |
| [case report: porcine circovirus type 2 infection in an european wild boar (sus scrofa) in the state of brandenburg, germany]. | this case represents the first case of porcine circovirus type 2 (pcv-2)--infection in a free living european wild boar associated with morphological lesions, which are regarded as characteristic for postweaning multisystemic wasting syndrome (pmws) in domestic pigs. the animal, an approximately 10 month old male, was found dead in a rural area within the state of brandenburg, germany. the closest commercial pig farm is located in 3 km distance from the spot where the carcass was found. at necro ... | 2003 | 14650740 |
| lack of o-antigen is essential for plasminogen activation by yersinia pestis and salmonella enterica. | the o-antigen of lipopolysaccharide (lps) is a virulence factor in enterobacterial infections, and the advantage of its genetic loss in the lethal pathogen yersinia pestis has remained unresolved. y. pestis and salmonella enterica express beta-barrel surface proteases of the omptin family that activate human plasminogen. plasminogen activation is central in pathogenesis of plague but has not, however, been found to be important in diarrhoeal disease. we observed that the presence of o-antigen re ... | 2004 | 14651623 |
| genes responsible for anaerobic fumarate and arginine metabolism are involved in growth suppression in salmonella enterica serovar typhimurium in vitro, without influencing colonisation inhibition in the chicken in vivo. | from a collection of over 2800 salmonella enterica subspecies enterica serotype typhimurium f98 tn5-tc1 insertion mutants 14 were identified as expressing growth-non-suppressive phenotype under strict anaerobic conditions. sequence analysis of regions flanking the tn insertions revealed that most of the selected mutants were defective in genes contributing to the anaerobic fumarate uptake and generation (insertions in dcua, dcub and aspa), or to the anaerobic l-arginine utilisation pathway (inse ... | 2003 | 14654290 |
| recovery of salmonella enterica from seropositive finishing pig herds. | the aim of this study was to assess the probability of detecting salmonella from pen faecal samples in seropositive classified finishing pig herds. the study involved 77 herds from denmark (20), the netherlands (20), greece (17) and germany (20). the serological herd status was determined by the blood-sampling of 50 finishing pigs. bacteriological sampling was performed by 20 pen faecal samples per herd. over-all, 47% of the blood samples had an od% larger than 10 and 23% larger than 40. salmone ... | 2003 | 14654291 |
| the attenuated sopb mutant of salmonella enterica serovar typhimurium has the same tissue distribution and host chemokine response as the wild type in bovine peyer's patches. | salmonella enterica serovar typhimurium is an important cause of enteric infections in farm animals and it is one of the most frequent food borne infections worldwide. serovar typhimurium lacking the sopb gene is attenuated for induction of host inflammatory response and fluid accumulation into the intestinal lumen, which correlates with clinical diarrhea. sopb is an inositol phosphate phosphatase, but its exact role in the pathogenesis of salmonellosis is still unclear. we employed the bovine i ... | 2003 | 14654296 |
| [genetic fingerprinting (pfge) of bacterial isolates for their molecular epidemiology]. | the determination of fragment patterns of bacterial genomic dna after digestion with long-range-cutting restriction enzymes and their separation in pulsed-field-gelelectrophoresis is currently regarded as reliable fingerprinting tool for surveillance and identification of infectious sources and routes of bacterial pathogens. the pfge based molecular fingerprinting has been applied and used for a broad range of bacterial species. as a prerequisite for its application in national and international ... | 2003 | 14655625 |
| th1-th2 response in hyperprolactinemic mice infected with salmonella enterica serovar typhimurium. | prolactin (prl) is a pituitary hormone and a cytokine known to regulate several physiological functions. it plays a role in modulating the immune system of rodents and humans. a hormonal protection against listeria and salmonella infections has been previously ascribed to effects of prl on immunocompetent cells. here, the role of prl in the th1-th2 response was evaluated based on the pattern of cytokines release by splenocytes from hyperprolactinemic mice infected with salmonella enterica serova ... | 2003 | 14656695 |
| lightcycler gyra mutation assay (gama) identifies heterogeneity in gyra in salmonella enterica serotypes typhi and paratyphi a with decreased susceptibility to ciprofloxacin. | mutations in gyra in strains of salmonella enterica serotypes typhi and paratyphi a have been characterised by a lightcycler-based pcr-hybridisation gyra mutation assay (gama) and by dna sequencing. four mutations (ser-83 to phe, asp-87 to asn, ser-83 to tyr and asp-87 to gly) have been identified in 13 strains of typhi and three strains of paratyphi a resistant to nalidixic acid (=nal(r)) and with decreased susceptibility to ciprofloxacin (=cp(l)), with the mutation ser-83 to phe predominating. ... | 2003 | 14659662 |
| single-copy green fluorescent protein gene fusions allow accurate measurement of salmonella gene expression in vitro and during infection of mammalian cells. | we developed a reliable and flexible green fluorescent protein (gfp)-based system for measuring gene expression in individual bacterial cells. until now, most systems have relied upon plasmid-borne gfp gene fusions, risking problems associated with plasmid instability. we show that a recently developed gfp variant, gfp+, is suitable for assessing bacterial gene expression. various gfp+ transcriptional fusions were constructed and integrated as single copies into the chromosome of salmonella ente ... | 2003 | 14660401 |
| detection of a salmonella enterica serovar california strain spreading in spanish feed mills and genetic characterization with dna microarrays. | we performed an epidemiological study on salmonella isolated from raw plant-based feed in spanish mills. overall, 32 different salmonella serovars were detected. despite its rare occurrence in humans and animals, salmonella enterica serovar california was found to be the predominant serovar in spanish feed mills. different typing techniques showed that isolates of this serovar were genetically closely related, and comparative genomic hybridization using microarray technology revealed 23 s. enter ... | 2003 | 14660409 |
| a salmonella protein causes macrophage cell death by inducing autophagy. | salmonella enterica, the causative agent of food poisoning and typhoid fever, induces programmed cell death in macrophages, a process found to be dependent on a type iii protein secretion system, and sipb, a protein with membrane fusion activity that is delivered into host cells by this system. when expressed in cultured cells, sipb caused the formation of and localized to unusual multimembrane structures. these structures resembled autophagosomes and contained both mitochondrial and endoplasmic ... | 2003 | 14662750 |
| salmonella rapidly kill dendritic cells via a caspase-1-dependent mechanism. | dendritic cells provide a critical link between innate and acquired immunity. in this study, we demonstrate that the bacterial pathogen salmonella enterica serovar typhimurium can efficiently kill these professional phagocytes via a mechanism that is dependent on sipb and the salmonella pathogenicity island 1-encoded type iii protein secretion system. rapid phosphatidylserine redistribution, caspase activation, and loss of plasma membrane integrity were characteristic of dendritic cells infected ... | 2003 | 14662878 |
| characterization of salmonella enterica serotype newport isolated from humans and food animals. | salmonella enterica serotype newport isolates resistant to at least nine antimicrobials (including extended-spectrum cephalosporins), known as serotype newport mdr-ampc isolates, have been rapidly emerging as pathogens in both animals and humans throughout the united states. resistance to extended-spectrum cephalosporins is associated with clinical failures, including death, in patients with systemic infections. in this study, 87 salmonella serotype newport strains were characterized by pulsed-f ... | 2003 | 14662912 |
| salmonella enterica pneumonia in a patient with lung cancer. | a case of life-threatening salmonella enterica serotype enteritidis pneumonia in a febrile patient with lung cancer is described. the organism was isolated from the sputum, the protected specimen brush material of bronchial secretions, and the stool. despite the early administration of appropriate and adequate treatment, the patient died 7 days after the onset of the infection. | 2003 | 14662992 |
| fatal case of salmonella enterica subsp. arizonae gastroenteritis in an infant with microcephaly. | salmonella enterica subsp. arizonae is a common gut inhabitant of reptiles, with snakes as the most common reservoir. though human cases due to this organism are exceedingly rare, it may infect young infants and immunocompromised individuals with a history of intimate associations with reptiles. gastroenteritis is the most common presentation; others include peritonitis, pleuritis, osteomyelitis, meningitis, and bacteremia. we report a fatal case of s. enterica subsp. arizonae gastroenteritis in ... | 2003 | 14662995 |