Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| progress on chicken t cell immunity to viruses. | avian virus infection remains one of the most important threats to the poultry industry. pathogens such as avian influenza virus (aiv), avian infectious bronchitis virus (ibv), and infectious bursal disease virus (ibdv) are normally controlled by antibodies specific for surface proteins and cellular immune responses. however, standard vaccines aimed at inducing neutralizing antibodies must be administered annually and can be rendered ineffective because immune-selective pressure results in the c ... | 2019 | 31101935 |
| avian influenza h9n2 subtype in ghana: virus characterization and evidence of co-infection. | between november 2017 and february 2018, ghanaian poultry producers reported to animal health authorities a dramatic increase in mortality rate and a relevant drop in egg production in several layer hen farms. laboratory investigations revealed that the farms had been infected by the h9n2 influenza subtype. virological and molecular characterization of the viruses identified in ghana is described here for the first time. whole genome analysis showed that the viruses belong to the g1-lineage and ... | 2019 | 31142135 |
| in vitro antiviral activity of fifteen plant extracts against avian infectious bronchitis virus. | avian infectious bronchitis (ib) is a disease that can result in huge economic losses in the poultry industry. the high level of mutations of the ib virus (ibv) leads to the emergence of new serotypes and genotypes, and limits the efficacy of routine prevention. medicinal plants, or substances derived from them, are being tested as options in the prevention of infectious diseases such as ib in many countries. the objective of this study was to investigate extracts of 15 selected medicinal plants ... | 2019 | 31142304 |
| combination of tlr2 and tlr3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken. | live intermediate plus infectious bursal disease virus (ibdv) vaccines (hot vaccines) are used for protection against the virulent ibdv strains in young chickens. we evaluated the potential of toll-like receptor (tlr) agonists to alleviate hot vaccine-induced immunosuppression. the combination of pam3csk4 and poly i:c synergistically upregulated ifn-β, ifn-γ, il-12, il-4, and il-13 transcripts and cross-inhibited il-1β, il-10, and inos transcripts in the chicken peripheral blood mononuclear cell ... | 2019 | 31160675 |
| genotyping of avian infectious bronchitis virus in afghanistan (2016-2017): the first report. | avian infectious bronchitis (ib) is a highly contagious viral disease which affects the poultry industry. the virus exists in a wide variety of genotypes, and phylogenetic analysis has been used to classify infectious bronchitis virus (ibv) strains. | 2019 | 31191702 |
| cross-species transmission of poultry pathogens in backyard farms: ducks as carriers of chicken viruses. | in backyard farms of lao people's democratic republic, mixed-species rearing of poultry is a breeding-ground for cross-species transmission. here, the epidemiology of viruses circulating among backyard poultry in vientiane province was assessed to guide future control strategies. oral/tracheal and cloacal swabs, collected from 605 poultry (308 ducks, 297 chickens) between 2011 and 2015, were screened by pcr for newcastle disease virus (ndv), coronavirus (cov) and chicken anaemia virus (cav). chi ... | 2019 | 31199168 |
| combined adjuvant effect of ginseng stem-leaf saponins and selenium on immune responses to a live bivalent vaccine of newcastle disease virus and infectious bronchitis virus in chickens. | vaccination with a live bivalent vaccine of newcastle disease virus (ndv) and infectious bronchitis virus (ibv) is a routine practice in poultry industry in china. this study was designed to evaluate ginseng stem-leaf saponins (gsls) in combination with selenium (se) for their adjuvant effect on the immune response to vaccination against ndv and ibv in chickens. a live bivalent vaccine of ndv and ibv was diluted in saline solution containing gsls or se or both and used to immunize chickens via a ... | 2019 | 31220864 |
| molecular characteristics and pathogenicity analysis of qx-like avian infectious bronchitis virus isolated in china in 2017 and 2018. | proportions of qx-like genotype infectious bronchitis virus (ibv) isolates have increased over time. here, to better understand the epidemiology and pathogenicity of ibv in china and control the spread of infectious bronchitis (ib), we conducted sequence analyses and examined the pathogenicity of 5 field isolates from diseased flocks in 2017 and 2018. sequence analyses revealed that all the 5 strains, as well as many recent field isolates from other researchers, belonged to the qx-like ibv genot ... | 2019 | 31222258 |
| comparative analysis of gene expression in virulent and attenuated strains of infectious bronchitis virus at subcodon resolution. | like all coronaviruses, avian infectious bronchitis virus (ibv) possesses a long, single-stranded, positive-sense rna genome (∼27 kb) and has a complex replication strategy that includes the production of a nested set of subgenomic mrnas (sgmrnas). here, we used whole-transcriptome sequencing (rnaseq) and ribosome profiling (riboseq) to delineate gene expression in the ibv m41-ck and beau-r strains at subcodon resolution. rnaseq facilitated a comparative analysis of viral rna synthesis and revea ... | 2019 | 31243124 |
| early vaccination of chickens induces suboptimal immunity against infectious bronchitis virus. | infectious bronchitis virus (ibv) is highly prevalent in broiler chickens despite extensive vaccination commonly conducted early after hatch. the effects of early vaccination on immune responses were further investigated in chickens primed at increasing ages, followed by booster vaccination with an attenuated arkansas (ark) delmarva poultry industry-type vaccine. results show that vaccination on day 1 of age elicits significantly lower systemic and mucosal antibody responses compared with vaccin ... | 2019 | 31251518 |
| infectious bronchitis virus vaccination at day 1 of age further limits cross protection. | cross-protection and immune responses elicited by infectious bronchitis virus (ibv) vaccination on day 1 of age or at later time points were examined. specific-pathogen-free chickens were vaccinated with a massachusetts-type vaccine and heterologous challenge was performed with an arkansas (ark) -type virulent strain. in trial 1, chickens vaccinated on day 1 or day 10 of age were challenged 21 days after vaccination. analysis of tracheal histopathology and viral load demonstrated less cross prot ... | 2019 | 31251531 |
| effects of chicken mhc haplotype on resistance to distantly related infectious bronchitis viruses. | the major histocompatibility complex (mhc) b locus of chickens has been associated with resistance to different viral diseases. we previously provided evidence that chicken lines expressing mhc haplotypes b2 and b19 exhibit different resistance to a challenge with infectious bronchitis virus (ibv) massachusetts 41 (m41). in the current study, we attempted to determine if those differences were true for genetically diverse ib viruses, i.e., ibv m41 and arkansas-delmarva poultry industry (arkdpi). ... | 2019 | 31251532 |
| molecular and biological characteristics of the infectious bronchitis virus tc07-2/gvi-1 lineage isolated in china. | in the present study, a thorough comparison of the infectious bronchitis virus (ibv) tc07-2/gvi-1 linage was conducted by comparing the s1 gene sequences of gvi-1 viruses with those of viruses representing the established genotypes and lineages. ibv gvi-1 strains were found to be closely genetically related to each other, irrespective of where the viruses were isolated, and differed from other known ibv genotypes and lineages; thus, it was confirmed that gvi represents a novel genotype. however, ... | 2019 | 31255833 |
| molecular epidemiology of infectious bronchitis virus and avian metapneumovirus in greece. | respiratory diseases like infectious bronchitis virus (ibv) and avian metapneumovirus (ampv) have been held accountable for major losses for poultry production. nevertheless, scarce information was present dealing with the prevalence and molecular epidemiology of these infections in greece and the efficacy of currently applied control strategies. to fill this gap, a specific epidemiological study was designed. a total of 106 broiler and layer farms, including 10 backyard and 96 commercial flocks ... | 2019 | 31264704 |
| molecular characterization of a unique variant of avian infectious bronchitis virus in tunisia. | avian infectious bronchitis virus (ibv) is responsible of significant economic losses for poultry industry around the world, through evolution of its pathogenicity, inadequacy of vaccines, and virus evasion. such evasion is related to the unstable nature of its rna, in particular the s glycoprotein encoding gene, which raises great challenges with regard to the control of the disease, along with the lack of proof reading mechanisms of the rna polymerase. the emergence of new variants might be a ... | 2019 | 31265109 |
| simultaneous and visual detection of infectious bronchitis virus and newcastle disease virus by multiple lamp and lateral flow dipstick. | infectious bronchitis virus (ibv) and newcastle disease virus (ndv) are both important viruses seriously affecting poultry industry worldwide. in this study, reverse-transcription lamp (rt-lamp) was combined with lateral flow dipstick (lfd) forming a novel detection tool which could simultaneously detect ibv and ndv visually. primers targeted the 5'-untranslated region (5'-utr) of ibv genome and the conserved region of ndv large polymerase gene (lp). the specificity and sensitivity of this multi ... | 2019 | 31265112 |
| microarray analysis of infectious bronchitis virus infection of chicken primary dendritic cells. | avian infectious bronchitis virus (ibv) is a major respiratory disease-causing agent in birds that leads to significant losses. dendritic cells (dcs) are specialised cells responsible for sampling antigens and presenting them to t cells, which also play an essential role in recognising and neutralising viruses. recent studies have suggested that non-coding rnas may regulate the functional program of dcs. expression of host non-coding rnas changes markedly during infectious bronchitis virus infec ... | 2019 | 31286855 |
| genotyping of avian infectious bronchitis virus in iran: detection of d274 and changing in the genotypes rate. | the coronavirus avian infectious bronchitis virus (ibv) poses economic threats to poultry farms worldwide, affecting the performance of both meat-type and egg-laying birds. to define the evolution of recent ibvs in iran, a genetic analysis based on hypervariable nucleotide sequences of s1 gene was carried out. tracheal swab samples were collected from 170 broiler flocks during 2017. ten tracheal swabs from each flock pooled. from a total number of 170 flocks tested, 84.71% found to be positive. ... | 2019 | 31300098 |
| viral interference between h9n2-low pathogenic avian influenza virus and avian infectious bronchitis virus vaccine strain h120 in vivo. | the interaction between a low pathogenic avian influenza virus (a/ck/tun/145/2012), a h9n2 tunisian isolate, and a vaccine strain (h120) of avian infectious bronchitis, administered simultaneously or sequentially three days apart to chicks during 20 days, was evaluated using elisa antibody levels, quantitative reverse transcription-polymerase chain reaction (qrt-pcr) analyses and histopathology examination. first, the in vivo replication interference of avian influenza virus (aiv) and infectious ... | 2019 | 31300117 |
| development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases. | avian influenza virus (aiv), infectious bronchitis virus (ibv), and newcastle disease virus (ndv) are important avian pathogens that can cause enormous economic loss on the poultry industry. different respiratory etiological agents may induce similar clinical signs that make differential diagnosis difficult. importantly, aiv brings about severe threat to human public health. therefore, a novel method that can distinguish these viruses quickly and simultaneously is urgently needed. | 2019 | 31324180 |
| molecular detection and genetic characterization of mycoplasma gallisepticum, mycoplama synoviae, and infectious bronchitis virus in poultry in myanmar. | in southeast asian countries, including myanmar, poultry farming is a major industry. in order to manage and maintain stable productivity, it is important to establish policies for biosecurity. infectious respiratory diseases are a major threat to poultry farming. avian influenza and newcastle disease have been reported in myanmar, but no scientific information is available for other respiratory pathogens, such as mycoplasmas and infectious bronchitis virus (ibv). identifying the genotypes and s ... | 2019 | 31345206 |
| a seminested rt-pcr for molecular genotyping of the brazilian br-i infectious bronchitis virus strain (gi-11). | infectious bronchitis (ib) is one of the avian diseases with the greatest impact on poultry farming worldwide. in brazil, strain br-i (gi-11) is the most prevalent in poultry flocks. the present study aimed to develop a seminested rt-pcr assay specific for the diagnosis of br-i ibv in brazilian samples, targeting subunit 1 of the s gene. the detection limit of this assay was 10 copies of the ibv genome. in this study, 62.24% of 572 organ pools from the 5 regions of brazil tested positive in a 3' ... | 2019 | 31365883 |
| emergence and genetic analysis of variant pathogenic 4/91 (serotype 793/b) infectious bronchitis virus in egypt during 2019. | infectious bronchitis virus (ibv) affects both vaccinated and unvaccinated flocks worldwide, with a significant impact on the poultry industry. the aim of the present study is to characterize an emerging variant pathogenic ibv originating from field outbreaks in vaccinated egyptian layer flock. samples were collected from disease-suspected flock with a history of administration of live and inactivated ibv vaccines (ma5 type). virus propagation in embryonated chicken eggs (eces), after three succ ... | 2019 | 31372921 |
| the c-terminal domain of the mers coronavirus m protein contains a trans-golgi network localization signal. | coronavirus m proteins represent the major protein component of the viral envelope. they play an essential role during viral assembly by interacting with all of the other structural proteins. coronaviruses bud into the endoplasmic reticulum (er)-golgi intermediate compartment (ergic), but the mechanisms by which m proteins are transported from their site of synthesis, the er, to the budding site remain poorly understood. here, we investigated the intracellular trafficking of the middle east resp ... | 2019 | 31399512 |
| protective effects of hypericin against infectious bronchitis virus induced apoptosis and reactive oxygen species in chicken embryo kidney cells. | avian infectious bronchitis virus (ibv), a coronavirus, causes infectious bronchitis leading to enormous economic loss in the poultry industry worldwide. hypericin (hy) is an excellent compound that has been investigated in antiviral, antineoplastic, and antidepressant. to investigate the inhibition effect of hy on ibv infection in chicken embryo kidney (cek) cells, 3 different experimental designs: pre-treatment of cells prior to ibv infection, direct treatment of ibv-infected cells, and pre-tr ... | 2019 | 31399732 |
| vaccine or field strains: the jigsaw pattern of infectious bronchitis virus molecular epidemiology in poland. | infectious bronchitis (ib), caused by infectious bronchitis virus (ibv), account for severe economic losses in the poultry industry. the continuous emergence of a multitude of ibv variants poses many challenges for its diagnosis and control, and live attenuated vaccines, despite their routine use, still plays a significant role in driving ibv evolution, further complicating the epidemiological scenario. unfortunately, the impact of different vaccination strategies on ib control, epidemiology, an ... | 2019 | 31399745 |
| co-infections of chickens with avian influenza virus h9n2 and moroccan italy 02 infectious bronchitis virus: effect on pathogenesis and protection conferred by different vaccination programmes. | since the emergence of low pathogenic avian influenza (lpai) h9n2 viruses in morocco in 2016, severe respiratory problems have been encountered in the field. infectious bronchitis virus (ibv) is often detected together with h9n2, suggesting disease exacerbation in cases of co-infections. this hypothesis was therefore tested and confirmed in laboratory conditions using specific-pathogen-free chickens. most common field vaccine programmes were then tested to compare their efficacies against these ... | 2020 | 31412705 |
| successful establishment of a reverse genetic system for qx-type infectious bronchitis virus and technical improvement of the rescue procedure. | in this study, a pathogenic avian infectious bronchitis virus (ibv) qx-type strain yn was successfully rescued by vaccinia virus based reverse genetic technology. ten fragments contiguously spanning the complete ibv genome were amplified and cloned into the vaccinia virus genome by homologous recombination. the full-length genomic cdna was transcribed in vitro, and its transcript was transfected into bhk-21/n cells that could stably express ibv n protein. at 48 h post transfection, the culture m ... | 2019 | 31430502 |
| first complete genome sequence of currently circulating infectious bronchitis virus strain dmv/1639 of the gi-17 lineage. | avian infectious bronchitis virus is the causative agent of a highly contagious disease that results in severe economic losses to the poultry industry worldwide. here, we report the first coding-complete genome sequence of strain dmv/1639 of the gi-17 lineage, isolated from broiler chickens in georgia in 2019. | 2019 | 31439703 |
| prevalence and molecular characterization of infectious bronchitis virus isolated from chicken in bangladesh. | the present study was aimed to determine the prevalence of infectious bronchitis virus (ibv) as well as virus isolation, identification, and molecular characterization of various strains circulating in bangladesh. | 2019 | 31440013 |
| ambient ammonia does not appear to inhibit the immune response to infectious bronchitis virus vaccination and protection from homologous challenge in broiler chickens. | commercial broilers are commonly exposed to gaseous ammonia (nh3) originating from degradation of nitrogen-containing excreta in the litter during the grow-out period. ammonia concentrations in the air are higher in poorly ventilated houses and appear to coincide with the elevated incidence of respiratory disease occurring during the winter months. this study examined the effect of nh3 on the immune response to infectious bronchitis virus (ibv) vaccination and protection against homologous serot ... | 2019 | 31472338 |
| molecular and antigenic characterization of gi-13 and gi-16 avian infectious bronchitis virus isolated in chile from 2009 to 2017 regarding 4/91 vaccine introduction. | the introduction of the 4/91 vaccine against infectious bronchitis in chile, a lineage not described until that time in the country, led to looking for changes induced by this action. this study considers eight isolates obtained from 2009, 2015 and 2017 and uses a maximum likelihood approach to classify the field isolates. three isolates were selected to analyze antigenic relationships through a virus neutralization test and to perform protection tests measured trough an rt-qpcr. the isolates fr ... | 2019 | 31491868 |
| peptides with 16r in s2 protein showed broad reactions with sera against different types of infectious bronchitis viruses. | vaccination plays a vital role in controlling diseases caused by chicken infectious bronchitis virus (ibv). the continuously variant antigenicity of ibv limits the application of current vaccine strategies and serological diagnostic systems. s2 protein is an invariant that harbors broad neutralizing epitopes. however, little is known about the key amino acids that contribute to the broad-spectrum s2 epitopes. in this study, we aimed to elucidate the specific amino acids contributing to s2 epitop ... | 2019 | 31500728 |
| reverse transcription recombinase-aided amplification assay combined with a lateral flow dipstick for detection of avian infectious bronchitis virus. | the study was conducted to develop a specific, simple, and sensitive method for diagnosis of avian infectious bronchitis virus (ibv). in this experiment, the selected downstream primer was labeled with biotin and the 5' end of raa probe was labeled with fam by reverse transcription recombinase-aided amplification (rt-raa) combined with lateral flow dipstick (lfd). a rt-raa-lfd assay that could be used for detection of ibv was established after optimization of rt-raa reaction time, reaction tempe ... | 2019 | 31539067 |
| recombinase polymerase amplification-nucleic acid lateral flow immunoassays for newcastle disease virus and infectious bronchitis virus detection. | newcastle disease virus (ndv) and infectious bronchitis virus (ibv) are two poultry pathogens affecting the respiratory tract of chickens, and cause major economic losses in the industry. rapid detection of these viruses is crucial to inform implementation of appropriate control measures. the objective of our study is developing a simple, rapid and field applicable recombinase polymerase amplification (rpa)-nucleic acid lateral flow (nalf) immunoassay for detection of ndv and ibv. isothermal amp ... | 2019 | 31549369 |
| rapid detection of avian leukosis virus using a fluorescent microsphere immunochromatographic test strip assay. | we developed a rapid fluorescent microsphere immunochromatographic test strip (fm-icts) assay for the quantitative detection of avian leukosis virus (alv). a monoclonal antibody specific for the alv major capsid protein encoded by the gag gene was coupled to label fluorescent microspheres. alv antibodies were coated on a nitrocellulose membrane to prepare a test line for sample detection. the fluorescence signals of the test and control lines can be read either visually by exposure to uv light o ... | 2019 | 31553793 |
| genetic analysis of avian coronavirus infectious bronchitis virus in yellow chickens in southern china over the past decade: revealing the changes of genetic diversity, dominant genotypes, and selection pressure. | the high mutation rates of infectious bronchitis virus (ibv) pose economic threats to the poultry industry. in order to track the genetic evolutionary of ibv isolates circulating in yellow chickens, we continued to conduct the genetic analyses of the structural genes s1, e, m, and n from 64 ibv isolates in southern china during 2009-2017. the results showed that the dominant genotypes based on the four genes had changed when compared with those during 1985-2008. based on the s1 gene phylogenetic ... | 2019 | 31561498 |
| rapid and sensitive real-time recombinase polymerase amplification for detection of marek's disease virus. | marek's disease (md) is one of the most devastating diseases of poultry. it's caused by the highly infectious alphaherpesvirus md virus serotype 1 (mdv-1). in this study, a rapid and easy-to-use assay based on recombinase polymerase amplification (rpa) was developed for mdv detection. primer-probe sets targeting the highly conserved region of meq gene were designed and applied to the rpa assay. the assay was carried out on a real-time thermostatic fluorescence detector at 39 °c for 20 min. as re ... | 2019 | 31580913 |
| comparative protective immunity provided by live vaccines of newcastle disease virus or avian metapneumovirus when co-administered alongside classical and variant strains of infectious bronchitis virus in day-old broiler chicks. | this study reports on the simultaneous administration of live ndv or ampv subtype b vaccines alongside two live ibv (massachusetts-h120 and 793b-cr88) vaccines in day-old maternal-antibody positive commercial broiler chicks. in the first experiment, chicks were divided into four groups; one unvaccinated and three groups vaccinated with live ndv vg/ga-avinew, live h120 + cr88, or vg/ga-avinew + h120 + cr88. in the second experiment, live ampv subtype b vaccine was used in place of ndv. clinical s ... | 2019 | 31607602 |
| molecular epidemiology of respiratory viruses in commercial chicken flocks in pakistan from 2014 through to 2016. | viral diseases are a matter of great concern for poultry farmers in pakistan. multiple common viral respiratory diseases (cvrds) cause huge economic losses in the poultry industry. the prevalence of cvrds in many countries, including pakistan, is not clearly understood. | 2019 | 31638995 |
| protective efficacy of different live attenuated infectious bronchitis virus vaccination regimes against challenge with ibv variant-2 circulating in the middle east. | six vaccination regimes using classical (mass-type) and variant (ib-var2 and ib-793b) live vaccines were evaluated against middle eastern variant-2 infectious bronchitis virus challenge. six groups of spf chicks (30 birds/group) were vaccinated using prime-boost regimes at day-1 and day-14 using; ib-m41:ib-var2, ib-var2:ib-var2, ib-var2:ib-m41, ib-ma5:ib-793b, ib-793b:ib-793b, and ib-793b:ib-ma5, respectively. ciliostasis and lesion scores were evaluated at day-5 after each vaccination. birds we ... | 2019 | 31649942 |
| the s2 subunit of qx-type infectious bronchitis coronavirus spike protein is an essential determinant of neurotropism. | some coronaviruses (covs) have an extra furin cleavage site (rrkr/s, furin-s2' site) upstream of the fusion peptide in the spike protein, which plays roles in virion adsorption and fusion. mutation of the s2' site of qx genotype (qx-type) infectious bronchitis virus (ibv) spike protein (s) in a recombinant virus background results in higher pathogenicity, pronounced neural symptoms and neurotropism when compared with conditions in wild-type ibv (wt-ibv) infected chickens. in this study, we prese ... | 2019 | 31652591 |
| emerging lethal infectious bronchitis coronavirus variants with multiorgan tropism. | infectious bronchitis virus (ibv) causes respiratory diseases in chickens and poses an economic threat to the poultry industry worldwide. despite vaccine use, there have been field outbreaks of ibv in taiwan. this study aimed to characterize the emerging ibv variants circulating in taiwan. the analysis of the structural protein genes showed that these variants emerged through frequent recombination events among taiwan strains, china strains, japan strains and vaccine strains. cross-neutralizatio ... | 2020 | 31682070 |
| a recombinant la sota vaccine strain expressing multiple epitopes of infectious bronchitis virus (ibv) protects specific pathogen-free (spf) chickens against ibv and ndv challenges. | infectious bronchitis (ib) and newcastle disease (nd) are two major infectious diseases that are a threat to the domestic poultry industry. in this study, we successfully generated a recombinant lasota candidate vaccine strain, rndv-ibv-t/b, which expresses a short, synthetic, previously identified ibv s1 multi-epitope cassette using the reverse genetic system. the recombinant virus was propagated in nine-day-old embryonated chicken eggs for 20 passages and genetic stability was confirmed by who ... | 2019 | 31683905 |
| the porcine deltacoronavirus replication organelle comprises double-membrane vesicles and zippered endoplasmic reticulum with double-membrane spherules. | porcine deltacoronavirus (pdcov) was first identified in hong kong in 2012 from samples taken from pigs in 2009. pdcov was subsequently identified in the usa in 2014 in pigs with a history of severe diarrhea. the virus has now been detected in pigs in several countries around the world. following the development of tissue culture adapted strains of pdcov, it is now possible to address questions regarding virus-host cell interactions for this genera of coronavirus. here, we presented a detailed s ... | 2019 | 31694296 |
| three amino acid changes in avian coronavirus spike protein allow binding to kidney tissue. | infectious bronchitis virus (ibv) infects ciliated epithelial cells in the chicken respiratory tract. while some ibv strains replicate locally, others can disseminate to various organs, including the kidney. here, we elucidate the determinants for kidney tropism by studying interactions between the receptor-binding domain (rbd) of the viral attachment protein spike from two ibv strains with different tropisms. recombinantly produced rbds from the nephropathogenic ibv strain qx and from the nonne ... | 2020 | 31694947 |
| evolution of infectious bronchitis virus in the field after homologous vaccination introduction. | despite the fact that vaccine resistance has been typically considered a rare phenomenon, some episodes of vaccine failure have been reported with increasing frequency in intensively-raised livestock. infectious bronchitis virus (ibv) is a widespread avian coronavirus, whose control relies mainly on extensive vaccine administration. unfortunately, the continuous emergence of new vaccine-immunity escaping variants prompts the development of new vaccines. in the present work, a molecular epidemiol ... | 2019 | 31706335 |
| infectious bronchitis virus mass-type (gi-1) and qx-like (gi-19) genotyping and vaccine differentiation using sybr green rt-qpcr paired with melting curve analysis. | infectious bronchitis virus (ibv) is a highly contagious virus of chicken, causing huge economic losses in the poultry industry. many genotypes circulate in a given area, and optimal protection relies on vaccination with live attenuated vaccines of the same genotype. as these live vaccines are derived from field viruses and circulate, understanding the prevalence of different ibv genotypes in any area is complex. in a recent study, the genome comparison of an ibv qx vaccine and its progenitor fi ... | 2020 | 31712091 |
| validation of specific quantitative real-time rt-pcr assay panel for infectious bronchitis using synthetic dna standards and clinical specimens. | infectious bronchitis (ib) is a highly contagious upper respiratory tract disease of chickens caused by infectious bronchitis virus (ibv), which has various serotypes that do not cross-protect. vaccine control strategies for this virus are only effective when designed around the currently circulating serotypes. it is essential to not only rapidly detect ibv but also to identify the type of virus causing disease. six taqman™-based quantitative real-time rt-pcr assays (universal, ark, mass, de/ga9 ... | 2020 | 31712094 |
| reverse-transcription recombinase-aided amplification assay for h7 subtype avian influenza virus. | h7 subtype avian influenza virus infection is an emerging zoonosis in some asian countries and an important avian disease worldwide. a rapid and simple test is needed to confirm infection in suspected cases during disease outbreaks. in this study, we developed a reverse-transcription recombinase-aided amplification assay for the detection of h7 subtype avian influenza virus. assays were performed at a single temperature (39°c), and the results were obtained within 20 min. the assay showed no cro ... | 2020 | 31714018 |
| antiviral activity against infectious bronchitis virus and bioactive components of hypericum perforatum l. | hypericum perforatum l., also known as saint john's wort, has been well studied for its chemical composition and pharmacological activity. in this study, the antiviral activities of h. perforatum on infectious bronchitis virus (ibv) were evaluated in vitro and in vivo for the first time. the results of in vitro experiments confirmed that the antiviral component of h. perforatum was ethyl acetate extraction section (hpe), and results showed that treatment with hpe significantly reduced the relati ... | 2019 | 31736754 |
| a multi-omics study of chicken infected by nephropathogenic infectious bronchitis virus. | chicken gout resulting from nephropathogenic infectious bronchitis virus (nibv) has become a serious kidney disease problem in chicken worldwide with alterations of the metabolic phenotypes in multiple metabolic pathways. to investigate the mechanisms in chicken responding to nibv infection, we examined the global transcriptomic and metabolomic profiles of the chicken's kidney using rna-seq and gc-tof/ms, respectively. furthermore, we analyzed the alterations in cecal microorganism composition i ... | 2019 | 31744152 |
| delmarva (dmv/1639) infectious bronchitis virus (ibv) variants isolated in eastern canada show evidence of recombination. | infectious bronchitis virus (ibv) infection in chickens can lead to an economically important disease, namely, infectious bronchitis (ib). new ibv variants are continuously emerging, which complicates vaccination-based ib control. in this study, five ibvs were isolated from clinical samples submitted to a diagnostic laboratory in ontario, canada, and subjected to detailed molecular characterization. analysis of the spike (s)1 gene showed that these five ibvs were highly related to the delmarva ( ... | 2019 | 31766215 |
| pathogenicity of a qx-like strain of infectious bronchitis virus and effects of accessory proteins 3a and 3b in chickens. | qx-like genotype infectious bronchitis virus (ibv) has become prevalent in recent years. few studies have reported the effects of accessory proteins 3a and 3b on pathogenicity in vivo. we developed a reverse genetics system to manipulate the genome of a qx-like ibv strain ibyz. recombinant viruses ribyz-scaug3a, ribyz-scaug3b and ribyz-scaug3ab were generated. these viruses do not express the accessory proteins 3a, 3b, or 3ab due to a mutation in the aug start codons. in spf embryonated eggs, th ... | 2019 | 31767070 |
| retraction note to: mitochondrial bioenergy alterations in avian hd11 macrophages infected with infectious bronchitis virus. | the editor-in-chief has retracted this article [1]. figures 1a, 1d and 2b (bottom right) are identical with figures 1a, 1h and 1b respectively in another article [2] which reports a study in a different species. in addition, table 1 contains data presented in a third article [3], which also reports a study in a different species. the editor-in-chief therefore no longer has confidence in the validity of the data and the conclusions drawn. tereza c. cardoso disagrees with this retraction. helena l ... | 2020 | 31784908 |
| attenuation and amino acid changes in infectious bronchitis virus iranian 793/b serotype during serial passaging in embryonated chicken eggs. | the use of live attenuated vaccine (lav) is the main method for controlling infectious bronchitis (ib). it is advisable to develop a lav using a dominant serotype in the region in the case of vaccine failure. since 793/b serotype is one of the most predominant circulating ib viruses in iran, attenuation of three iranian 793/b isolates (ir/773/2001, ir/794/2002 and ir/520/2002) was done by serial passaging in specific pathogen free (spf) embryonated chicken eggs up to 90 passages to assess the de ... | 2019 | 31802683 |
| attenuated live infectious bronchitis virus qx vaccine disseminates slowly to target organs distant from the site of inoculation. | infectious bronchitis (ib) is a highly contagious respiratory disease of poultry, caused by the avian coronavirus infectious bronchitis virus (ibv). currently, one of the most relevant genotypes circulating worldwide is ibv-qx (gi-19), for which vaccines have been developed by passaging virulent qx strains in embryonated chicken eggs. here we explored the attenuated phenotype of a commercially available qx live vaccine, ib primo qx, in specific pathogens free broilers. at hatch, birds were inocu ... | 2020 | 31822427 |
| evaluation of bacteriophage efficacy in reducing the impact of single and mixed infections with escherichia coli and infectious bronchitis in chickens. | infectious bronchitis virus (ibv) represents a major threat to poultry production worldwide particularly when complicated with bacterial infection. in the present study samples were collected from forty broiler farms with respiratory manifestations to characterize ibv and e. coli. bacteriophages were isolated and enriched from sampled farms to study its efficacy to control single and mixed infections with e. coli and ibv in vivo. twelve out of forty farms were positive for ibv. phylogenetic anal ... | 2019 | 31839902 |
| antigenic and pathogenic characteristics of qx-type avian infectious bronchitis virus strains isolated in southwestern china. | the qx-type avian infectious bronchitis virus (ibv) is still a prevalent genotype in southwestern china. to analyze the antigenicity and pathogenicity characteristics of the dominant genotype strains (qx-type), s1 gene sequence analysis, virus cross-neutralization tests, and pathogenicity test of eight qx-type ibv isolates were conducted. sequence analysis showed that the nucleotide homology between the eight strains was high, but distantly related to h120 and 4/91 vaccine strains. cross-neutral ... | 2019 | 31847269 |
| a simple and rapid diagnostic method to detect new goose astrovirus using reverse-transcription loop-mediated isothermal amplification. | in this study, we develop a reverse-transcription loop-mediated isothermal amplification (rt-lamp) assay for rapid and easy detection of goose astroviruses (gastvs) in clinical samples. the specific lamp primer sets were designed targeting the orf2 gene of gastv. the conditions of lamp amplification were optimized in terms of reaction time and temperature. the optimal conditions are 60 min in a 60 °c water bath. no cross-reactivity was noted with fowl adenovirus serotype 4 (fadv-4), duck tembusu ... | 2020 | 31879584 |
| effect of ibv d1466 on egg production and egg quality and the effect of heterologous priming to increase the efficacy of an inactivated ibv vaccine. | to protect layers, breeders and grandparents against damage by infectious bronchitis virus infections during the laying period, vaccination using live priming followed by a boost with inactivated ib vaccine is commonly used. for many ib variants, homologous live vaccines are not available for priming. very little is known about the efficacy of priming with heterologous live ib vaccines (or combination of live ib vaccines) to induce broad ib protection in long-living chickens. in this study, the ... | 2020 | 31899942 |
| antiviral responses against chicken respiratory infections: focus on avian influenza virus and infectious bronchitis virus. | some of the respiratory viral infections in chickens pose a significant threat to the poultry industry and public health. in response to viral infections, host innate responses provide the first line of defense against viruses, which often act even before the establishment of the infection. host cells sense the presence of viral components through germinal encoded pattern recognition receptors (prrs). the engagement of prrs with pathogen-associated molecular patterns leads to the induction of pr ... | 2020 | 31901597 |
| genetic, antigenic and pathogenic characterization of avian coronaviruses isolated from pheasants (phasianus colchicus) in china. | two viruses were isolated in 2017 from commercial pheasants with severe clinical signs and mortality in shandong and anhui provinces, china, respectively. we examined the pathogenic effects of the viruses in chicken embryos and the size and morphology of the virus particles, performed phylogenetic analysis based on the s1 gene and complete genomic sequences, and examined the antibody responses against infectious bronchitis virus (ibv). the results suggested that the viruses i0623/17 and i0710/17 ... | 2020 | 31902509 |
| molecular epidemiology of infectious bronchitis virus in poland from 1980 to 2017. | the presence of infectious bronchitis virus (ibv) was identified for the first time in the poultry population in poland at the end of the 1960s. from this time a few waves of epidemics caused by different ibv variants spread across the country. in order to gain more insight into the molecular epidemiology of ibv in poland, in the present study the s1 coding region of 34 ibv isolates and nearly whole genome of 10 strains collected over a period of 38 years was characterized. phylogenetic analysis ... | 2020 | 31917362 |
| author correction: a recombinant newcastle disease virus (ndv) expressing s protein of infectious bronchitis virus (ibv) protects chickens against ibv and ndv. | an amendment to this paper has been published and can be accessed via a link at the top of the paper. | 2020 | 31937880 |
| molecular characterization of variant infectious bronchitis virus in china, 2019: implications for control programmes. | infectious bronchitis virus (ibv), an ongoing emergence enveloped virus with a single-stranded positive-sense rna genome, belongs to the gammacoronavirus genus in the coronaviridae family. ibv-associated tracheitis, nephritis, salpingitis, proventriculitis and egg drop have caused devastating economic losses to poultry industry worldwide. since the end of 2018, a remarkably increasing number of commercial broilers and layers, vaccinated or not, were infected with ibv in china. here, we described ... | 2020 | 31943814 |
| characterization of infectious bronchitis virus d181, a new serotype (gii-2). | this paper describes the characterization of a new infectious bronchitis virus (ibv) strain d181, that rapidly evolved from a low-level incidental finding in 2017 to become the second most isolated ibv strain in dutch layers and breeders in 2018, as well as being found in samples from germany and belgium. based on the sequence of the s gene and the results of cross-neutralization tests, d181 can be considered as a new serotype and the second lineage within genotype ii (gii-2). the experimental i ... | 2020 | 31951468 |
| qx-like infectious bronchitis virus reported in india. | 2020 | 31953290 | |
| characterization of an outbreak of infectious coryza (avibacterium paragallinarum) in commercial chickens in central california. | in 2017, the turlock branch of the california animal health & food safety laboratory system received a significant increase in infectious coryza (ic) necropsy cases, with a total of 54 submissions originating from commercial broilers (n = 40), commercial layers (n = 11), and backyard chickens (n = 3). layer flocks positive for ic were distributed within the adjacent counties of merced and stanislaus, while broiler flocks were concentrated within merced county. the backyard flocks were located in ... | 2019 | 31967433 |
| gga-mir-30d regulates infectious bronchitis virus infection by targeting usp47 in hd11 cells. | avian infectious bronchitis virus (ibv) is a coronavirus which infects chickens and causes severe economic losses to the poultry industry worldwide. micrornas (mirnas) are important intracellular regulators and play a pivotal role in viral infections. in previous studies, we have revealed that ibv infection caused a significant down-regulation of gga-mir-30d expression in chicken kidneys. in present study, we investigated the role of gga-mir-30d in the process of ibv infection of hd11 cell line ... | 2020 | 31982568 |
| a recombinant infectious bronchitis virus from a chicken with a spike gene closely related to that of a turkey coronavirus. | using viral metagenomics, the complete genome sequence of an infectious bronchitis virus (ibv) strain (named ahysx-1) from a fecal sample from a healthy chicken in anhui province, china, was determined. the genome sequence of ahysx-1 was found to be very similar to that of ibv strain ck/ch/lln/131040 (kx252787), except for the spike gene region, which is similar to that of a turkey coronavirus strain (eu022526), suggesting that ahysx-1 is a recombinant. recombination analysis and phylogenetic an ... | 2020 | 31982944 |
| regulation of immunity-related genes by infectious bronchitis virus challenge in spleen of laying chickens. | understanding of host pathogen interactions is important in planning strategies for effective control of the pathogen. the present study investigated the regulation of genes involved in the activation of splenic immune system in mature laying chickens challenged with t strain of infectious bronchitis virus (ibv). among all the genes studied, the relative expression levels of fas cell surface death receptor (fas), interleukin 7 (il7), il18, proteasome subunit alpha 3 (psma3), major histocompatibi ... | 2020 | 31985363 |
| isolation and characterization of avian coronavirus from healthy eclectus parrots (eclectus roratus) from indonesia. | avian coronavirus has a wide range of hosts, from chickens and turkeys to wild birds. this virus causes an economically and, possibly, environmentally, important loss in the poultry industry. therefore, research into the avian coronavirus in various species of birds is required. the eclectus parrot (eclectus roratus) is an endemic bird to indonesia and northern australia and often kept as pets. at present, there has been limited information about avian coronavirus infection among birds. this stu ... | 2019 | 32009759 |
| mirna repertoire and host immune factor regulation upon avian coronavirus infection in eggs. | avian infectious bronchitis virus (ibv) is a coronavirus with great economic impact on the poultry industry, causing an acute and highly contagious disease in chickens that primarily affects the respiratory and reproductive systems. the cellular regulation of ibv pathogenesis and the host immune responses involved remain to be fully elucidated. micrornas (mirnas) have emerged as a class of crucial regulators of numerous cellular processes, including responses to viral infections. here, we employ ... | 2020 | 32025807 |
| research note: first evidence of infectious bronchitis virus middle-east gi-23 lineage (var2-like) in germany. | the infectious bronchitis virus middle-east gi-23 lineage (var2-like) was observed on a german broiler farm, for the first time. the animals suffered from respiratory and nephropathogenic disease. gross lesions observed during necropsy included tracheitis, aerosacculitis, and nephritis. tracheal swabs were tested positive for infectious bronchitis virus middle-east gi-23 lineage (var2-like) by pcr. furthermore, sequence analysis of the s1 spike protein showed close relationship to the commercial ... | 2020 | 32036979 |
| change of pathotype and phylogenetic analysis of infectious bronchitis virus detected in kagoshima prefecture, japan. | infectious bronchitis (ib) is a highly contagious disease in chickens, induced by ib virus (ibv) infection. the pathotype and s1 genotype of ibv field strain that was detected from 2008 to 2018 were investigated in kagoshima prefecture, japan. the frequency of cases that the renal lesion characteristic of ibv infection was histopathologically confirmed was significantly higher from 2014 to 2018 than from 2008 to 2009, suggesting the altered pathotype of ibv. of 7 genotypes (jp-i, jp-ii, jp-iii, ... | 2020 | 32037366 |
| recombinant infectious bronchitis coronavirus h120 with the spike protein s1 gene of the nephropathogenic ibyz strain remains attenuated but induces protective immunity. | infectious bronchitis (ib) is a highly infectious viral disease responsible for major economic losses in the poultry industry. a reverse genetic vaccine is a safe, rapid, and effective method of achieving ib prevention and control. in this study, we constructed the recombinant strain, rh120-s1/yz, using a reverse genetic system, based on the backbone of the h120 vaccine strain, with the s1 gene replaced with that of the qx-like nephropathogenic strain, ck/ch/ibyz/2011, isolated in china. the res ... | 2020 | 32057575 |
| effect of tlr agonist on infections bronchitis virus replication and cytokine expression in embryonated chicken eggs. | avian infectious bronchitis (ib) is an acute, highly infectious and contagious viral disease of chickens caused by avian infectious bronchitis virus (ibv) belonging to the genus coronavirus and family coronaviridae. it can affect all age groups of birds. the toll-like receptors (tlrs) are a major class of innate immune pattern recognition receptors that have a key role in immune response and defense against various infections.the tlrs are essential for initiation of innate immune responses and i ... | 2020 | 32065987 |
| pathological and virological analysis of concurrent disease of chicken anemia virus infection and infectious bronchitis in japanese native chicks. | a concurrent infection of chicken anemia virus (cav) and infectious bronchitis virus (ibv) was detected in japanese native chicks in 2017, in which a high mortality rate (97.7%) was recorded in a small flock of 130 chicks exhibiting poor growth. histological examination revealed that the affected chicks exhibited two different pathological entities: one was severe hematopoietic and lymphocytic depletion with abnormally large cells containing intranuclear inclusion bodies of cav, whereas the othe ... | 2020 | 32074517 |
| expression and immunoreactivity of a recombinant multi-epitope antigen designed based on four major structural proteins of avian infectious bronchitis virus. | the development of rapid, simple, and sensitive diagnostic methods for identification of avian infectious bronchitis virus (ibv) is crucial for the effective control of avian infectious bronchitis. in the present study, a tandemly arranged multiepitope peptide (named semn) was designed with four antigenic regions derived from four major structural proteins of ibv. then, we performed codon optimization of semn gene by changing the codon-adaptation index from 0.45 to 0.94 and expressed the optimiz ... | 2020 | 32089981 |
| molecular survey and interaction of common respiratory pathogens in chicken flocks (field perspective). | the present study was designed for the detection of the most prevalent respiratory infections in chicken flocks and clarifying their interaction and impact on flock health. | 2019 | 32095050 |
| 16s rrna gene sequencing reveals an altered composition of the gut microbiota in chickens infected with a nephropathogenic infectious bronchitis virus. | infectious bronchitis virus (ibv), a member of the coronaviridae family, causes serious losses to the poultry industry. intestinal microbiota play an important role in chicken health and contribute to the defence against colonization by invading pathogens. the aim of this study was to investigate the link between the intestinal microbiome and nephropathogenic ibv (nibv) infection. initially, chickens were randomly distributed into 2 groups: the normal group (inc) and the infected group (iibv). t ... | 2020 | 32103130 |
| complete genome sequence of avian coronavirus strain ga08 (gi-27 lineage). | avian coronavirus, also known as infectious bronchitis virus, is a highly contagious respiratory pathogen of chickens that is responsible for major economic losses to the poultry industry around the globe. here, we report the complete genome sequence of strain ga08 of the gi-27 lineage, isolated from a fecal sample from a broiler chicken collected in georgia in 2015. | 2020 | 32107296 |
| a novel low virulent respiratory infectious bronchitis virus originating from the recombination of qx, tw and 4/91 genotype strains in china. | in china, variants of infectious bronchitis virus (ibv) evolve continually and diverse recombinant strains have been reported. here, an ibv strain, designated as ck/ch/ljx/2017/07 (referred as jx17) was isolated from chicken vaccinated with h120 and 4/91 in jiangxi, china, in 2017. sequence analysis reveals of the s1 gene of jx17 the highest nucleotide identity of 98.15% with that of gi-7 genotype tw2575/98 strain. furthermore, whole genome analysis among jx17 and other 18 ibv strains demonstrat ... | 2020 | 32122588 |
| real-time, minion-based, amplicon sequencing for lineage typing of infectious bronchitis virus from upper respiratory samples. | infectious bronchitis (ib) causes significant economic losses in the global poultry industry. control of ib is hindered by the genetic diversity of the causative agent, infectious bronchitis virus (ibv), which has led to the emergence of several serotypes that lack complete serologic cross-protection. although serotyping requires immunologic characterization, genotyping is an efficient means to identify ibvs detected in samples. sanger sequencing of the s1 subunit of the spike gene is currently ... | 2020 | 32133932 |
| pathogenicity and genome changes in qx-like infectious bronchitis virus during continuous passaging in embryonated chicken eggs. | infectious bronchitis (ib) remains a major problem in the global poultry industry despite the many available vaccines. live attenuated vaccines are the most effective means of preventing ib and are traditionally generated by serial passaging of a wild strain in embryonated chicken eggs. in this study, the sz isolate of the qx-like infectious bronchitis virus (ibv) was continuously passaged in chicken embryos for 250 passages. we compared the pathogenicity of different passages (sz50, sz100, sz15 ... | 2020 | 32142742 |
| n-glycosylation of infectious bronchitis virus m41 spike determines receptor specificity. | infection of chicken coronavirus infectious bronchitis virus (ibv) is initiated by binding of the viral heavily n-glycosylated attachment protein spike to the alpha-2,3-linked sialic acid receptor neu5ac. previously, we have shown that n-glycosylation of recombinantly expressed receptor binding domain (rbd) of the spike of ibv-m41 is of critical importance for binding to chicken trachea tissue. here we investigated the role of n-glycosylation of the rbd on receptor specificity and virus replicat ... | 2020 | 32213247 |
| optimization of transfection methods for huh-7 and vero cells: a comparative study. | availability of an efficient transfection protocol is the first determinant in success of gene transferring studies in mammalian cells which is accomplished experimentally for every single cell type. herein, we provide data of a comparative study on optimization of transfection condition by electroporation and chemical methods for huh-7 and vero cells. different cell confluencies, dna/reagent ratios and total transfection volumes were optimized for two chemical reagents including jetpei™ and lip ... | 2012 | 32214542 |
| genome sequencing and characterization analysis of a beijing isolate of chicken coronavirus infectious bronchitis virus. | avian infectious bronchitis virus (aibv) is classified as a member of the genus coronavirus in the family coronaviridae. the enveloped virus has a positive-sense, single-stranded rna genome of approximately 28 kilo-bases, which has a 5' cap structure and 3' polyadenylation tract. the complete genome sequence of infectious bronchitis virus (ibv), beijing isolate, was determined by cloning sequencing and primer walking. the whole genome is 27733 nucleotides in length, has ten open reading frames: ... | 2004 | 32214717 |
| diverse gammacoronaviruses detected in wild birds from madagascar. | to date, infectious bronchitis virus (ibv) is potentially found in wild birds of different species. this work reports the survey of coronaviruses in wild birds from madagascar based on the targeting of a conserved genome sequence among different groups of covs. phylogenetic analyses revealed the presence of gammacoronaviruses in different species of gruiformes, passeriformes, ciconiiformes, anseriformes, and charadriiformes. furthermore, some sequences were related to various ibv strains. aquati ... | 2015 | 32214942 |
| evaluation of sialic acid and acute-phase proteins (haptoglobin and serum amyloids a) in healthy and avian infection bronchitis virus-infected chicks. | forty-five 24-day-old cobb chicks infected with infectious bronchitis virus (ibv) and ten healthy 24-day-old cobb chicks without any clinical signs of ibv as control group were selected for the study. all of the diseased chicks showed some or all of the clinical signs of infectious bronchitis including gasping, coughing and nasal discharge, wet eyes, swollen sinuses, reduction of food consumption and weight gain. diagnosis of ibv was based on clinical signs and elisa test. blood samples were tak ... | 2011 | 32214968 |
| detection of 793/b serotype of infectious bronchitis virus in tissue sample by indirect immunoperoxidase assay. | the indirect immunoperoxidase (iip) assay was compared with the reverse transcription-polymerase chain reaction (rt-pcr) for detection of 793/b serotype of infectious bronchitis virus in tissues samples collected from experimentally infected chickens. this technique was optimized in specific pathogen-free (spf)-embryonated chicken eggs and broiler chickens inoculated with the iranian ir/773/2001 strain of 793/b serotype the trachea, lung, kidney, and cecal tonsil tissue samples from experimental ... | 2014 | 32214974 |
| rapid detection of avian infectious bronchitis virus by reverse transcriptase-loop mediated isothermal amplification. | a reverse-transcription loop mediated isothermal amplification (rt-lamp) was developed for rapid diagnosis of infectious bronchitis (ib) in poultry by targeting the spike protein 2 gene (s2). rt-lamp primers were designed for ibv-s2 targets and optimized to run at 60 °c for 45 min. as compared with rt-pcr, rt-lamp was 100 times more sensitive for ibv-s2 gene. rt-lamp showed specific amplification with ib viral genome but not with other avian respiratory pathogens due to their mismatching with ib ... | 2015 | 32226206 |
| genetic sequence changes related to the attenuation of avian infectious bronchitis virus strain tw2575/98. | the attenuated avian infectious bronchitis virus (ibv), derived from a wild strain (tw2575/98w) in chicken embryos after 75 passages, is designed as a commercial vaccine strain (tw2575/98vac) to control the disease in taiwan. the differences in viral infectivity, replication efficiency, and genome sequences between tw2575/98w and tw2575/98vac were determined and compared. tw2575/98vac caused earlier death of chicken embryos and had higher viral replication efficiency. thirty amino acid substitut ... | 2020 | 32232712 |
| genetic characterization of infectious bronchitis viruses in thailand, 2014-2016: identification of a novel recombinant variant. | infectious bronchitis (ib) causes severe economic losses to the poultry industry worldwide owing to frequent emergence of novel infectious bronchitis virus (ibv) variants, which potentially affect the effectiveness of the currently used ibv vaccine. therefore, continuous monitoring of ibv genotypes and lineages recently circulating in chickens worldwide is essential. in this study, we characterized the complete s1 gene from 120 ibvs circulating in chickens in thailand from 2014 to 2016. phylogen ... | 2020 | 32241468 |
| multiple recombination events between field and vaccine strains resulted in the emergence of a novel infectious bronchitis virus with decreased pathogenicity and altered replication capacity. | in this study, we isolated and identified 2 infectious bronchitis virus (ibv) strains from layer chickens soon after vaccination with the massachusetts-connecticut bivalent vaccine (conn) and h120 and 4/91 booster vaccines in china in 2011. the results of cross-virus-neutralization tests and phylogenetic analysis of the s1 subunit of spike gene of these vaccine strains and other reference strains showed that strain ljl/110302 was of gi-19 lineage, whereas lln/111169 was of the gi-1 lineage of th ... | 2020 | 32241473 |
| protection following simultaneous vaccination with three or four different attenuated live vaccine types against infectious bronchitis virus. | two or more different live attenuated infectious bronchitis virus (ibv) vaccine types are often given to broilers to induce homologous protection as well as to broaden protection against other ibv types in the field. however, the ability of broilers to respond to three or four different antigenic types of ibv vaccine has not been examined experimentally. in this study, we vaccinated one-day-old broiler chicks by eyedrop with three or four different ibv vaccine types simultaneously. the presence ... | 2020 | 32242456 |
| intestinal tropism of an infectious bronchitis virus isolate not explained by spike protein binding specificity. | an infectious bronchitis virus (ibv) with an unusual enteric tropism (calent) was isolated from a california broiler flock exhibiting runting-stunting syndrome. ibv was detected in the small intestine, but not in the respiratory tract or kidney. during virus isolation in embryos, it did not replicate in chorioallantoic membrane (cam) but could be recovered from intestines. its s1 protein showed 93% amino acid sequence identity to a california variant isolated in 1999 (cal99). intestinal lesions ... | 2020 | 32267122 |
| cytokine responses in tracheas from major histocompatibility complex congenic chicken lines with distinct susceptibilities to infectious bronchitis virus. | the chicken major histocompatibility complex (mhc) b locus has been linked to resistance to infectious diseases. we have previously provided evidence that the mhc congenic chicken lines 331/b2 and 335/b19 differ in susceptibility to infectious bronchitis virus (ibv) strains m41 and arkdpi in in vivo challenge experiments. innate immune responses can be difficult to measure in vivo because they are nonspecific and can be triggered by environmental factors. in an attempt to address this issue, we ... | 2020 | 32267123 |
| limited protection conferred by recombinant newcastle disease virus expressing infectious bronchitis spike protein. | recombinant newcastle disease virus (ndv) lasota (ls) expressing secreted trimeric spike (s)-ectodomain (se) of infectious bronchitis virus (ibv) (rls/ibv.se) was developed and evaluated for protection conferred against ibv challenge. the ibv s-ectodomain protein, which is s excluding the transmembrane anchor and short cytoplasmic domain of s2, expressed from recombinant ls corresponds to an arkansas (ark)-type ibv. in a first experiment, chickens were primed at 1 day of age or primed at 1 day o ... | 2020 | 32267125 |